pycom02g06510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002922
Physical Location & Seq
Reverse (-)
4522544 .. 4525144
2601 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g06510.2

Sequence Viewer

Length: 543 bp
ATGGCGGAATCCAAACCTGGACCAGCTCAAGCTCCACCTGATAAGGAAGTAAAGGCGCCTAATTTGATTGAAAGAGCAAAGGAAGAGACGGGGGCAGTGTTGCACACTGACAAAATATATCATGATCACAAGGAAACTCATGGATTGCGTGATGATATCAATGAGAAAACCCCACTGGACGATGTCAAAGCTCCGAATATGTTCGAGAGGGCGAAGGAGGAGTTTGAGGCCATAGTTGAAGCAATCCATCCCAAGAAAGAGTCTCCCACTTATGGAAAAATGTTGGTTTTAGTTTGCCCCTTCGAACAAATTGGAAATCTGAACTTGATGTTTGCAGGAAATCATGCTAAGACATCCAATTTGATTGAGAAAGCCAAGCAAAAGATTGAGACATTGATGCACCATGAGAAGTCGCCAAAGAATCATGATAACGAAACTCCTGAGAAGTCACCGAAGTATCATACTAAAGAAACTCATGGAAGGAGTGATGACATTGACGAGAGTATCCCAATCGATCGATGTCAAGGCTCCGAATGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.37

Weight (kDa)

5.77

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 55
AciI CCGC 1 cut(s) 5
AcyI GRCGYC 1 cut(s) 56
AfiI CCNNNNNNNGG 1 cut(s) 272
AgsI TTSAA 2 cut(s) 71, 239
AjnI CCWGG 1 cut(s) 16
AluBI AGCT 3 cut(s) 26, 32, 191
AluI AGCT 3 cut(s) 26, 32, 191
Alw26I GTCTC 3 cut(s) 80, 267, 383
AoxI GGCC 1 cut(s) 228
ArsI GACNNNNNNTTYG 2 cut(s) 343, 375
Asp700I GAANNNNTTC 1 cut(s) 200
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 1 cut(s) 441
AsuII TTCGAA 1 cut(s) 303
AvaII GGWCC 1 cut(s) 20
BaeI ACNNNNGTAYC 4 cut(s) 440, 473, 487, 520
BanI GGYRCC 1 cut(s) 55
BccI CCATC 1 cut(s) 255
BciT130I CCWGG 1 cut(s) 18
BciVI GTATCC 1 cut(s) 515
BclI TGATCA 1 cut(s) 124
BcoDI GTCTC 3 cut(s) 80, 267, 383
BfoI RGCGCY 1 cut(s) 59
BfuI GTATCC 1 cut(s) 515
Bme1390I CCNGG 1 cut(s) 18
Bme18I GGWCC 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 2 cut(s) 57, 529
BmrFI CCNGG 1 cut(s) 18
BmsI GCATC 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 303
BpuEI CTTGAG 1 cut(s) 12
Bsa29I ATCGAT 2 cut(s) 513, 517
BsaHI GRCGYC 1 cut(s) 56
Bsc4I CCNNNNNNNGG 1 cut(s) 272
Bse1I ACTGG 1 cut(s) 180
BseBI CCWGG 1 cut(s) 18
BseCI ATCGAT 2 cut(s) 513, 517
BseGI GGATG 2 cut(s) 247, 353
BseLI CCNNNNNNNGG 1 cut(s) 272
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 1 cut(s) 180
BseRI GAGGAG 1 cut(s) 233
Bsh1285I CGRYCG 1 cut(s) 517
BshFI GGCC 1 cut(s) 230
BshNI GGYRCC 1 cut(s) 55
BshVI ATCGAT 2 cut(s) 513, 517
BsiEI CGRYCG 1 cut(s) 517
BslI CCNNNNNNNGG 1 cut(s) 272
BsmAI GTCTC 3 cut(s) 80, 267, 383
BsmBI CGTCTC 1 cut(s) 80
BsnI GGCC 1 cut(s) 230
Bsp119I TTCGAA 1 cut(s) 303
Bsp143I GATC 2 cut(s) 124, 514
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 1 cut(s) 433
BspDI ATCGAT 2 cut(s) 513, 517
BspHI TCATGA 2 cut(s) 121, 424
BspLI GGNNCC 2 cut(s) 57, 529
BspT104I TTCGAA 1 cut(s) 303
BspT107I GGYRCC 1 cut(s) 55
BsrI ACTGG 1 cut(s) 180
BssMI GATC 2 cut(s) 124, 514
BssNI GRCGYC 1 cut(s) 56
Bst2UI CCWGG 1 cut(s) 18
Bst6I CTCTTC 1 cut(s) 78
BstACI GRCGYC 1 cut(s) 56
BstBI TTCGAA 1 cut(s) 303
BstDEI CTNAG 2 cut(s) 348, 441
BstF5I GGATG 2 cut(s) 247, 353
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 2 cut(s) 127, 517
BstMAI GTCTC 3 cut(s) 80, 267, 383
BstMBI GATC 2 cut(s) 124, 514
BstMCI CGRYCG 1 cut(s) 517
BstNI CCWGG 1 cut(s) 18
BstSCI CCNGG 1 cut(s) 16
Bsu15I ATCGAT 2 cut(s) 513, 517
BsuI GTATCC 1 cut(s) 515
BsuRI GGCC 1 cut(s) 230
BsuTUI ATCGAT 2 cut(s) 513, 517
BtsCI GGATG 2 cut(s) 247, 353
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 3 cut(s) 102, 105, 173
CciI TCATGA 2 cut(s) 121, 424
CfoI GCGC 1 cut(s) 58
Cfr13I GGNCC 1 cut(s) 20
ClaI ATCGAT 2 cut(s) 513, 517
CviAII CATG 6 cut(s) 122, 140, 344, 404, 425, 476
CviJI RGCY 6 cut(s) 26, 32, 191, 230, 374, 528
CviKI_1 RGCY 6 cut(s) 26, 32, 191, 230, 374, 528
DdeI CTNAG 2 cut(s) 348, 441
DinI GGCGCC 1 cut(s) 57
DpnI GATC 2 cut(s) 126, 516
DpnII GATC 2 cut(s) 124, 514
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
EciI GGCGGA 1 cut(s) 20
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 20
EcoRII CCWGG 1 cut(s) 16
EcoRV GATATC 1 cut(s) 157
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
Esp3I CGTCTC 1 cut(s) 80
FaeI CATG 6 cut(s) 125, 143, 347, 407, 428, 479
FatI CATG 6 cut(s) 121, 139, 343, 403, 424, 475
FbaI TGATCA 1 cut(s) 124
FokI GGATG 2 cut(s) 234, 340
GlaI GCGC 1 cut(s) 57
HaeII RGCGCY 1 cut(s) 59
HaeIII GGCC 1 cut(s) 230
HhaI GCGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 6 cut(s) 125, 143, 347, 407, 428, 479
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HinfI GANTC 3 cut(s) 8, 260, 421
HphI GGTGA 1 cut(s) 441
Hpy188I TCNGA 3 cut(s) 195, 321, 532
Hpy188III TCNNGA 4 cut(s) 122, 205, 425, 440
HpyAV CCTTC 3 cut(s) 208, 310, 474
HpyCH4V TGCA 3 cut(s) 103, 335, 400
HpyF3I CTNAG 2 cut(s) 348, 441
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 6 cut(s) 125, 143, 347, 407, 428, 479
HspAI GCGC 1 cut(s) 56
KasI GGCGCC 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 2 cut(s) 124, 514
LmnI GCTCC 3 cut(s) 37, 196, 533
LpnPI CCDG 7 cut(s) 3, 30, 36, 51, 161, 321, 453
LweI GCATC 1 cut(s) 387
MaeIII GTNAC 1 cut(s) 447
MalI GATC 2 cut(s) 126, 516
MboI GATC 2 cut(s) 124, 514
MboII GAAGA 1 cut(s) 95
MluCI AATT 3 cut(s) 61, 309, 358
Mly113I GGCGCC 1 cut(s) 56
MlyI GAGTC 1 cut(s) 269
MnlI CCTC 3 cut(s) 201, 211, 220
MroXI GAANNNNTTC 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 18
MvaI CCWGG 1 cut(s) 18
NarI GGCGCC 1 cut(s) 56
NdeII GATC 2 cut(s) 124, 514
NlaIII CATG 6 cut(s) 125, 143, 347, 407, 428, 479
NlaIV GGNNCC 2 cut(s) 57, 529
NmuCI GTSAC 1 cut(s) 447
NspV TTCGAA 1 cut(s) 303
PagI TCATGA 2 cut(s) 121, 424
PdmI GAANNNNTTC 1 cut(s) 200
PfeI GAWTC 2 cut(s) 8, 421
PflFI GACNNNGTC 1 cut(s) 182
Ple19I CGATCG 1 cut(s) 517
PleI GAGTC 1 cut(s) 268
PluTI GGCGCC 1 cut(s) 59
PpsI GAGTC 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 16
PspGI CCWGG 1 cut(s) 16
PspN4I GGNNCC 2 cut(s) 57, 529
PspPI GGNCC 1 cut(s) 20
PsyI GACNNNGTC 1 cut(s) 182
PvuI CGATCG 1 cut(s) 517
Sau3AI GATC 2 cut(s) 124, 514
Sau96I GGNCC 1 cut(s) 20
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 1 cut(s) 18
SetI ASST 5 cut(s) 19, 28, 34, 40, 193
SfaNI GCATC 1 cut(s) 387
SfoI GGCGCC 1 cut(s) 57
SfuI TTCGAA 1 cut(s) 303
SinI GGWCC 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
Sse9I AATT 3 cut(s) 61, 309, 358
SsiI CCGC 1 cut(s) 5
SspDI GGCGCC 1 cut(s) 55
StyD4I CCNGG 1 cut(s) 16
TaqI TCGA 4 cut(s) 204, 303, 513, 517
TasI AATT 3 cut(s) 61, 309, 358
TfiI GAWTC 2 cut(s) 8, 421
TscAI CASTG 3 cut(s) 102, 112, 180
TseFI GTSAC 1 cut(s) 447
Tsp45I GTSAC 1 cut(s) 447
TspRI CASTG 3 cut(s) 102, 112, 180
Tth111I GACNNNGTC 1 cut(s) 182
VpaK11BI GGWCC 1 cut(s) 20
XmnI GAANNNNTTC 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.