pycom02g06700
ERF Family

Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008043
Physical Location & Seq
Reverse (-)
4631209 .. 4632738
1530 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 612 bp
ATGTGCAGCTGCTCTTCCTTGGCGTCCCTTCTCATCCCACCACCCACTCTTCCTCTCCAACTCAAGGAGGATGGGGTTAGAGTCAGCACTGTGCTTACGAACCCATCGTCTAAAATTGACCGGACCGATCTGAGAAGTTGCAGACGTCACGGACGGTGTTTTGACACGAAAGCTAAGGTGACCGTTGAAGAAGATGTACTGGAGAAACAATCAGTTGGTGCTCAGGACAAAATTGATTTTGGAGTTCTTAGCGTCCACCATGTCGGATTGTTGTGTGAAAACCTTGAAAGATCTCTCAGCTTTTACCAGAACATTTTAGGTCTTGAAATAAATGAGGCTAGGCCACATGACAAGCTCCCCTTCAGAGGTGCCTGGTTATGGGTTGGTTCAGAGATGATTCATCTCATGGAACTTCCAAATCCCGACCCTTTACCCGGAAGACCCGCCCATGGAGGTCGCGACCGCCATGCTTGTATTGCAATCAAGGACGTATCGAAGCTCAAAGCAATCCTCGACGACGCTGGTATTACCTACACGCTTAGCAAGTCCGGGAGGCCCGCGATTTTTACACGAGACCCGGACGCAAATGCTCTGGAGTTTACTCAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

204

Amino Acids

22.43

Weight (kDa)

6.65

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 85 - 200 9.8e-11 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 148
AccB1I GGYRCC 1 cut(s) 368
AccII CGCG 2 cut(s) 459, 560
AciI CCGC 3 cut(s) 444, 463, 558
AcuI CTGAAG 1 cut(s) 346
AcyI GRCGYC 2 cut(s) 23, 145
AfaI GTAC 1 cut(s) 198
AfiI CCNNNNNNNGG 5 cut(s) 64, 365, 378, 434, 449
AgsI TTSAA 3 cut(s) 188, 287, 326
AjnI CCWGG 1 cut(s) 371
AluBI AGCT 5 cut(s) 9, 173, 300, 355, 499
AluI AGCT 5 cut(s) 9, 173, 300, 355, 499
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 567
AoxI GGCC 2 cut(s) 341, 554
ApeKI GCWGC 2 cut(s) 6, 9
ArsI GACNNNNNNTTYG 2 cut(s) 92, 124
AspS9I GGNCC 2 cut(s) 123, 555
AsuC2I CCSGG 3 cut(s) 435, 550, 578
AsuHPI GGTGA 1 cut(s) 190
AvaII GGWCC 1 cut(s) 123
BanI GGYRCC 1 cut(s) 368
BarI GAAGNNNNNNTAC 2 cut(s) 180, 212
BauI CACGAG 1 cut(s) 570
BbsI GAAGAC 1 cut(s) 445
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 1 cut(s) 18
BccI CCATC 2 cut(s) 65, 112
BcgI CGANNNNNNTGC 2 cut(s) 449, 483
BciT130I CCWGG 1 cut(s) 373
BcnI CCSGG 3 cut(s) 435, 550, 578
BcoDI GTCTC 1 cut(s) 567
BfaI CTAG 1 cut(s) 339
BglII AGATCT 1 cut(s) 290
BisI GCNGC 2 cut(s) 7, 10
BlpI GCTNAGC 1 cut(s) 539
BlsI GCNGC 2 cut(s) 8, 11
Bme1390I CCNGG 4 cut(s) 373, 435, 550, 578
Bme18I GGWCC 1 cut(s) 123
BmgT120I GGNCC 2 cut(s) 123, 555
BmiI GGNNCC 1 cut(s) 370
BmrFI CCNGG 4 cut(s) 373, 435, 550, 578
BpiI GAAGAC 1 cut(s) 445
BpmI CTGGAG 1 cut(s) 221
Bpu10I CCTNAGC 2 cut(s) 174, 222
Bpu1102I GCTNAGC 1 cut(s) 539
BpuEI CTTGAG 2 cut(s) 47, 588
BpuMI CCSGG 3 cut(s) 435, 550, 578
BsaHI GRCGYC 2 cut(s) 23, 145
BsaI GGTCTC 1 cut(s) 567
BsaJI CCNNGG 2 cut(s) 18, 448
BsaWI WCCGGW 1 cut(s) 120
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 365, 378, 434, 449
Bse1I ACTGG 1 cut(s) 204
BseBI CCWGG 1 cut(s) 373
BseDI CCNNGG 2 cut(s) 18, 448
BseGI GGATG 2 cut(s) 33, 76
BseLI CCNNNNNNNGG 5 cut(s) 64, 365, 378, 434, 449
BseMII CTCAG 3 cut(s) 122, 236, 310
BseNI ACTGG 1 cut(s) 204
BseXI GCAGC 1 cut(s) 18
BsgI GTGCAG 1 cut(s) 25
Bsh1236I CGCG 2 cut(s) 459, 560
Bsh1285I CGRYCG 1 cut(s) 463
BshFI GGCC 2 cut(s) 343, 556
BshNI GGYRCC 1 cut(s) 368
BsiEI CGRYCG 1 cut(s) 463
BsiHKAI GWGCWC 1 cut(s) 223
BsiSI CCGG 4 cut(s) 121, 435, 549, 578
BslFI GGGAC 1 cut(s) 10
BslI CCNNNNNNNGG 5 cut(s) 64, 365, 378, 434, 449
BsmAI GTCTC 1 cut(s) 567
BsmFI GGGAC 1 cut(s) 10
BsnI GGCC 2 cut(s) 343, 556
Bso31I GGTCTC 1 cut(s) 567
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 2 cut(s) 127, 290
Bsp1720I GCTNAGC 1 cut(s) 539
Bsp19I CCATGG 1 cut(s) 448
Bsp68I TCGCGA 1 cut(s) 459
BspACI CCGC 3 cut(s) 444, 463, 558
BspANI GGCC 2 cut(s) 343, 556
BspCNI CTCAG 3 cut(s) 123, 235, 309
BspFNI CGCG 2 cut(s) 459, 560
BspLI GGNNCC 1 cut(s) 370
BspQI GCTCTTC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 368
BspTNI GGTCTC 1 cut(s) 567
BsrI ACTGG 1 cut(s) 204
BssECI CCNNGG 2 cut(s) 18, 448
BssMI GATC 2 cut(s) 127, 290
BssNI GRCGYC 2 cut(s) 23, 145
BssSI CACGAG 1 cut(s) 570
BssT1I CCWWGG 2 cut(s) 18, 448
Bst2BI CACGAG 1 cut(s) 570
Bst2UI CCWGG 1 cut(s) 373
Bst4CI ACNGT 3 cut(s) 91, 156, 184
Bst6I CTCTTC 2 cut(s) 19, 54
BstACI GRCGYC 2 cut(s) 23, 145
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 6 cut(s) 131, 174, 222, 248, 296, 539
BstDSI CCRYGG 1 cut(s) 448
BstEII GGTNACC 1 cut(s) 178
BstF5I GGATG 2 cut(s) 33, 76
BstFNI CGCG 2 cut(s) 459, 560
BstKTI GATC 2 cut(s) 130, 293
BstMAI GTCTC 1 cut(s) 567
BstMBI GATC 2 cut(s) 127, 290
BstMCI CGRYCG 1 cut(s) 463
BstMWI GCNNNNNNNGC 1 cut(s) 476
BstNI CCWGG 1 cut(s) 373
BstPI GGTNACC 1 cut(s) 178
BstSCI CCNGG 4 cut(s) 371, 433, 548, 576
BstUI CGCG 2 cut(s) 459, 560
BstV1I GCAGC 1 cut(s) 18
BstV2I GAAGAC 1 cut(s) 445
BstX2I RGATCY 1 cut(s) 290
BstYI RGATCY 1 cut(s) 290
BsuRI GGCC 2 cut(s) 343, 556
BtgI CCRYGG 1 cut(s) 448
BtsCI GGATG 2 cut(s) 33, 76
BtsIMutI CAGTG 1 cut(s) 87
BtuMI TCGCGA 1 cut(s) 459
Cac8I GCNNGC 1 cut(s) 558
Cfr13I GGNCC 2 cut(s) 123, 555
CpoI CGGWCCG 1 cut(s) 123
CseI GACGC 4 cut(s) 12, 241, 527, 590
Csp6I GTAC 1 cut(s) 197
CspI CGGWCCG 1 cut(s) 123
CviAII CATG 5 cut(s) 260, 347, 406, 449, 467
CviJI RGCY 8 cut(s) 9, 173, 300, 338, 343, 355, 499, 556
CviKI_1 RGCY 8 cut(s) 9, 173, 300, 338, 343, 355, 499, 556
CviQI GTAC 1 cut(s) 197
DdeI CTNAG 6 cut(s) 131, 174, 222, 248, 296, 539
DpnI GATC 2 cut(s) 129, 292
DpnII GATC 2 cut(s) 127, 290
Eam1104I CTCTTC 2 cut(s) 19, 54
EarI CTCTTC 2 cut(s) 19, 54
Eco130I CCWWGG 2 cut(s) 18, 448
Eco31I GGTCTC 1 cut(s) 567
Eco47I GGWCC 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 346
Eco91I GGTNACC 1 cut(s) 178
EcoO65I GGTNACC 1 cut(s) 178
EcoRII CCWGG 1 cut(s) 371
EcoT14I CCWWGG 2 cut(s) 18, 448
ErhI CCWWGG 2 cut(s) 18, 448
FaeI CATG 5 cut(s) 263, 350, 409, 452, 470
FaiI YATR 6 cut(s) 261, 348, 379, 407, 450, 468
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FaqI GGGAC 1 cut(s) 10
FatI CATG 5 cut(s) 259, 346, 405, 448, 466
FauI CCCGC 2 cut(s) 451, 565
Fnu4HI GCNGC 2 cut(s) 7, 10
FokI GGATG 2 cut(s) 20, 83
Fsp4HI GCNGC 2 cut(s) 7, 10
FspBI CTAG 1 cut(s) 339
GluI GCNGC 2 cut(s) 7, 10
GsuI CTGGAG 1 cut(s) 221
HaeIII GGCC 2 cut(s) 343, 556
HapII CCGG 4 cut(s) 121, 435, 549, 578
HgaI GACGC 4 cut(s) 12, 241, 527, 590
Hin1I GRCGYC 2 cut(s) 23, 145
Hin1II CATG 5 cut(s) 263, 350, 409, 452, 470
HinfI GANTC 2 cut(s) 81, 397
HpaII CCGG 4 cut(s) 121, 435, 549, 578
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 2 cut(s) 256, 600
Hpy188I TCNGA 4 cut(s) 132, 266, 365, 391
Hpy188III TCNNGA 5 cut(s) 224, 323, 422, 458, 593
Hpy8I GTNNAC 2 cut(s) 256, 600
Hpy99I CGWCG 2 cut(s) 518, 521
HpyAV CCTTC 2 cut(s) 38, 370
HpyCH4III ACNGT 3 cut(s) 91, 156, 184
HpyCH4IV ACGT 2 cut(s) 145, 489
HpyCH4V TGCA 3 cut(s) 6, 141, 479
HpyF10VI GCNNNNNNNGC 1 cut(s) 476
HpyF3I CTNAG 6 cut(s) 131, 174, 222, 248, 296, 539
HpySE526I ACGT 2 cut(s) 145, 489
Hsp92I GRCGYC 2 cut(s) 23, 145
Hsp92II CATG 5 cut(s) 263, 350, 409, 452, 470
Kzo9I GATC 2 cut(s) 127, 290
LguI GCTCTTC 1 cut(s) 19
LmnI GCTCC 1 cut(s) 360
Lsp1109I GCAGC 1 cut(s) 18
MaeI CTAG 1 cut(s) 339
MaeII ACGT 2 cut(s) 145, 489
MaeIII GTNAC 2 cut(s) 146, 178
MalI GATC 2 cut(s) 129, 292
MboI GATC 2 cut(s) 127, 290
MboII GAAGA 5 cut(s) 6, 41, 200, 203, 450
MflI RGATCY 1 cut(s) 290
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 2 cut(s) 114, 231
MlyI GAGTC 1 cut(s) 90
MmeI TCCRAC 2 cut(s) 82, 244
MnlI CCTC 7 cut(s) 61, 63, 328, 359, 446, 521, 546
MspA1I CMGCKG 1 cut(s) 9
MspI CCGG 4 cut(s) 121, 435, 549, 578
MspR9I CCNGG 4 cut(s) 373, 435, 550, 578
MvaI CCWGG 1 cut(s) 373
MvnI CGCG 2 cut(s) 459, 560
MwoI GCNNNNNNNGC 1 cut(s) 476
NciI CCSGG 3 cut(s) 435, 550, 578
NcoI CCATGG 1 cut(s) 448
NdeII GATC 2 cut(s) 127, 290
NlaIII CATG 5 cut(s) 263, 350, 409, 452, 470
NlaIV GGNNCC 1 cut(s) 370
NmuCI GTSAC 2 cut(s) 146, 178
NruI TCGCGA 1 cut(s) 459
PciSI GCTCTTC 1 cut(s) 19
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PfeI GAWTC 1 cut(s) 397
PfoI TCCNGGA 1 cut(s) 548
PkrI GCNGC 2 cut(s) 8, 11
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 371
PspEI GGTNACC 1 cut(s) 178
PspGI CCWGG 1 cut(s) 371
PspN4I GGNNCC 1 cut(s) 370
PspPI GGNCC 2 cut(s) 123, 555
PsuI RGATCY 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 9
RruI TCGCGA 1 cut(s) 459
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
Rsr2I CGGWCCG 1 cut(s) 123
RsrII CGGWCCG 1 cut(s) 123
SapI GCTCTTC 1 cut(s) 19
SatI GCNGC 2 cut(s) 7, 10
Sau3AI GATC 2 cut(s) 127, 290
Sau96I GGNCC 2 cut(s) 123, 555
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 4 cut(s) 373, 435, 550, 578
SduI GDGCHC 1 cut(s) 223
SinI GGWCC 1 cut(s) 123
SmlI CTYRAG 2 cut(s) 62, 603
SmoI CTYRAG 2 cut(s) 62, 603
Sse9I AATT 2 cut(s) 114, 231
SsiI CCGC 3 cut(s) 444, 463, 558
SspMI CTAG 1 cut(s) 339
StyD4I CCNGG 4 cut(s) 371, 433, 548, 576
StyI CCWWGG 2 cut(s) 18, 448
TaaI ACNGT 3 cut(s) 91, 156, 184
TaiI ACGT 2 cut(s) 148, 492
TaqI TCGA 2 cut(s) 494, 513
TaqII GACCGA 1 cut(s) 140
TasI AATT 2 cut(s) 114, 231
TatI WGTACW 1 cut(s) 196
TfiI GAWTC 1 cut(s) 397
TscAI CASTG 1 cut(s) 94
TseFI GTSAC 2 cut(s) 146, 178
TseI GCWGC 2 cut(s) 6, 9
Tsp45I GTSAC 2 cut(s) 146, 178
TspDTI ATGAA 1 cut(s) 389
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 123
XspI CTAG 1 cut(s) 339
ZraI GACGTC 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.