pycom02g12560

regulation of parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0004207
Physical Location & Seq
Reverse (-)
9196752 .. 9211153
14402 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 7569 bp
ATGTTCCCGAACACCGCAGCGCGACGGAGACTGGCTTCTGTGCTGCGGCCATGGCTCCAGGAAGAGCCCGAACTCGATCTCAAATTAGGGGTTATCAATTCCCACGCGGTCGCCAGGAACCTCCGGTTCGACACTGGGGCTCTCAACGAGCTCTTCGACGAATCGGCCGAGTTTTCCTTCTCAGAGGTCACTGTCGAACACTTGAGCGTTCGGTTCTCGAACTGGCTCGTCCCTGCCTTCTCAATCGAATTTCATGGCGTCAGTGTGGAGCTAGCAGTTCGGGAGGAGAAAAATTTAAAGCGAAAACCGAGGGAGGAATATGCAGAAGATGTGAAGAAGAAGAAGCTTTCGGAGATAGATCCCGAGGGTAGTGCTTTACATGGTGTTTTTGAAAAGCTTGTGGCCACTTCTACTCCAACAAAAAGTTTTAAAGACACCCTTTGGAATCTCTTACTGAAACACTGTCAGCTTCGAGTGCAAGATATCAATGTACAAGTTAAGGTTCCCATCTTAAATGATTCACTTGTTTGTGTGTTCGACGTAAGAGATATCAATGCAGATCCTCAGTATCTTGATCACAGGTGTCTTCTTCGAGGTCTATTTGGTGCACTCTTTCTACCTTTGAAAGAGAGCTCTTTTGTTATAGCAGGCAGTAGTTTTGAGGTGGGGTTCAAGAGAGAAAATCAGCTCAAAAACGTGTTGTTATTGACTGATTTGTGCACTTGCATTAGATTAAATAATTTTCAGCTCGTGGATGTAAATCTTCAATTTCCAGAATTATGTTGTACATTTTCTCCGGATGACATTTATTGGTATTCAGCATTGGGTAAATTGTCATCCCAAGGATCTCGTTCTGCTAGAAATGGTGTCCAACTATGGAAACTAGCTGCAAGAAATATGAACAACTTGACTTCAGGTTCTAGATGGTCGTTGCAAAAATTAGTTGGTCTTGTTTGTTTATGGCTACGTTATGCTAATGCTTACGAGCAGTTATTGTTAGCAATTGGATTTTCTGATGATCACTCATTGAAGAGATCAGCTGCTAGAATTTCTCAGGACAAGATGTTTTTGGCTTCTGTAAAGAACCAATGCAAGGTGATTTTTGATATAGAGAAACAGTTGCCGGCTGAAGCCATTGTACAGGCACGGAGAATCGCCCGTCATAGAGCAGCATCAAATGCTCAATCTACCAAAGATAGTTTAAAAAAATCCTCCGTGATTACTCACTTCGACTTCCTTCTCAAGATCCTTCTGGTACTAGCCTGTACCTGGAGAGTTCTGTGCAAGATATTCCACTTTATCATACACTTGTTAACTTTTAGGAAAGTATTGGACGAAGAGCCAGACAATGCAAATTTGGATATTGTTTCTGGAGGTCCGTGTAAAGATTTTTGCTTCATCGTGATCTTTGGAAATGTTCTAATCACTATTTCTCATATAAATGAAGTTCAAATCTCAGTAAATAAACAATTAGAATCACATATTGGAATCTCTTGTTCAGATTTCCTTTCATTTCGTCTTTCAGTTGATTCGCTGTTCTTAAAATATGTAGAAGACATGTGTGAACAGTCTGTACTTGTATCATGTGGGCAGTTGAAGGTTAGATCCTCATCTCTTTCGAAAGCTTCTGTTAGAGAAATCATTTCAAATGGGGAAGAACATTGGAAGGAGAGTAATGATGATTTGAAGAATATTCTGTGGGGTGAGCCAGCACAAACTTTTCCCCTTTCAGAAACTTACGAAACTGCTTATGCTGATCATGCGGAAGGTGCTTGTGCCTCATTTTTGAAAACATTTCTTGGAGACATGTGGTTGAACTGGAAAAGATCCTGCAAAAAATTTGAAAAAAGTGAGATTCAGTACTTTGAAAACCCATTCCTTCTCTGTGAAATTAAAAACTGGACATATCCAGACCTCAAAAACTCAGAGTCTGGGCTTTCGAAGTTGTTTTTGACCATGGGAAAGTTGAATCTAGTTTTGGGATGTCCCTCCATACTATCGGTATCTCTGCTCTTAAAGCAAATACAACATGCTCTATGCTGGACGGAGGACAATGGTCAGTCAGGGGTTCTGCTACATTCTCCAAGAGCTAGTAATGATAGCATTTGCACGCACTATGCTAATAGATTGGAAATGGCATTGGTTAAGATGCTTCCAGAGAAACATGTTCAAATAGGGATATTTGCAGCTGGTCCTCACATCCGTATGTCATTGGGAAAGAGTTTTGGTCGTGGCAGCAAAGACAAAAATCATCAAGTGAGTCAGGAGGATTTTCACCTTGCATTTGATGTGCACAGCATAGAGGCTGCAGTATGGCCAACCTCACAATTTGATCTGGCATCATTTGTTGCACCCTCAGGAGCTGATGATGTAGAAACAAAGTGCCTCAGGTTGGAGAAGCCTCTCGGAGTTGATAAATTTAAATCAGATAATGGAAAGTATCAATCTCAGGGGTCAGTTTCTCTTGGTTTTTATTTACACGTTGATGGTTTAGATGCTTACCTGATCGACTCAGCAGGGAAAAAACAAAGTGACATTTTAGTTCTGAAGCCAATGACTGTGTGGATGTCATCCTTAAGGGTGTATGCTCATTCATTTAGCACAAATTTTGTTGCTCTCTCAGCAGCTTTCTATGGGACAGCTAAAGGATTCACTATTTTGTCATATACAGATGAGATTTATGTTTTCCTTCAGGTGCTTGCCAATTTATCTTCTGCAATGTCATATTCATTTAGCAGTTTTGCAGCTCTAAGTTACTCGCCTTTTAAGTTTGCAATACAAGAATTTGCTTTTCCTGAATCTGAGAATGTGGAGACAACTGTACATGGAGCTCCCTTCAATTATAGCAGTTTCCTAGTTTCATTTAATGGGTCCTTCAAACTCAAGTCTATTGATATCATTCTTCACAAGTCAAGGATCAGTGGTAATCTGAAGAGTTGTGTAGGAACCTCTGATGTTTCAAGTAGCAAAAAGTTAGCTGATCATGACTTGCCTGACTGTGGCATTTGGATATCAATTCACCAAACAACTGCTGATATATCTTCAACAGAAGGAAAAGGGAAAGTTATTTCTTATTTATCAGAAATCCAGTCTATAATTTTTAGATATAAGAATCAAAAAGGAGAAAGTATGGATCATTCTGCACATGGTGATCTACTGCAATCTCTTGATTGCTTATATGAACTCTCACTTTCTAGTTGTGTATTTCATTTATTGCTGCCTCTCTCTCAGAATAACCCATCTTCAGGTAGTGTTAGTAATGCACTTGGTACCTCAACTTCTGTTGGTGAAATAGTGCACGTGGAAAATCTTCCATTCACCACTGATTCTGAAAGCCCAAATGGTCAGAACTGTAGCTTTTTTCAGGAGACAGAATTTGCTTCAAATATTCCACCACCTGATGCAAGTCATTGGTTACTGATAAATGTTACATTGGGTTATATTTATATGGGAAGGTACTCAACAAAGAATGTTATGAACGGAGATCAATTAAACAAGTTTGTGTCTTCAGTTTATGTTGGTGGTGAATTTCAGACAATCTGTTGCGGAATTCAGGGTGGTTTTCTGTTCCTTGAAATAAAAGCTTTGGCTACCTTCATCAACTGTTTTGCTTCATATCGTCTTTGTTTTGCAAATCTTGTATCTTGTTTACAATCGTCTGATGAACACATTGAAGAAGCTGCAGTTTCTGTGCATATGACCAGACAAAGTAATCATTGTATCGACGAATATATGCAAGAGGCTCACTGTACATCTCCACATGCCAAGATCACACAGATGGAAGCTTTCATCTTAAATCTATCTCAGTTTTCTTGTGCTCTTTTGATTGAAGACGAAAATGGTGGTTTGCAGGAAATTGTTCTTGAGGTTGATGTCCAGTTTAAGTTTCAATTGGAAAACATGAGAAGAAAACTTATATTTGATCTTTCTCGCTTGTCAATACTTTCTAGGGTCTTTCAAGAAATTGTGGAAAATGAAATCCAAATTTCTCATTTTTCTGTTAATGGTGCGTGTTCTTCAAGAGGTCCTGGTTCAGAGGATGAGTATTCTGTACCTAATTCCCTGCCTGAGGCATTCCGTCTAAGTCATGTCGTGAAGCATGCAGGTGCTCTCATGTCAGTTGAGATGCCCTTAAGTGATCCTTTGTGCTTGAATGAGGTTTGGGTTGGAAGTGGATCTATTTCAGGTTTCGATATCACAATATCCCTATCTCAAATACAGGTGCTTTTGTCTATGATTTCATCCTTCTCTGAGGTAACCAAAAAAGAGATGGTCAGTGAATCGGATAGAAGGCACATGTCCAGTGGCGAGGAATTCAAAAACAGTATGGAGACGTTGGTTCCGAACGGTGCAATAGTGGCAATTCAAGATGTCCATCAGCACATGTATTTTTCCGTTGAGGGGGAGGAGAATAAGTACAGTGTGGCGGGTGCAGTTCATTATTCTCTTGTTGGGGAAAGGGCTCTTTTCAGGGTCAAGTATCAAAATCAAGGGAGATGGAAGTCATCAGTTTCATGGTTCTCCTTGATATCATTGTATGCTAAGAATGGCACAGGGGAGCCTTTGCGCTTGAATTACCGTCCAGGATCTGGTTTTGTTGACTTATCTAGCGCTAATGATAATAAGTGGGCACTTTGGAGAGCACTTTCTTGTGAGCCTGAAAATACCGACGGTGACATTGACTGGGAGCCCAACAATCAATTGGTTCAAAAGACATTTTACCTCTTGAACAAGAAGAATGACTCTGCTGTTGCTTTCGTAGATGGGATTCCAGAGTTTGTTCCCAAGCCTGGAAATCCATTCAAGTTGAAAGTGTTCCATAATGTTTCTGTAGCTCGTGATGTAAAGATGGATAGTTACCTTGGGGAGGCATCTGTAACTGGTCTGCAACATGATGCACTCAGGGATGATGGAAAAACTTCTGCGAGAAGTGGAAAGCTTCCTTGCATTGATATTACCTTTGACAATATTTCTTTGACCGTTTTCCATGAACTTGTAGATACAAAAAACATGTTTCCTCTCCTTCGTGGTTGCATTGACAGAACCAAACTTACTGTCCAAATTCTCCCTTCTAAAACCAGGGTGATAAGCACGTCAACTGCACTTCTAGATTACTTTGATGCACAAAAAAACTTATGGAGAGAGATTCTCCACCCAGTTGAAATATGCATCTTCTATCGTTCTAGCTTCCAGCTTCAGGGATCTGAAGCTGTTTCGCGAGTACCTGTTCATATTCACTGCAGAACTAAAGAGTTGAATGTATCCCTATCCGAGCTTTCAATGGACATACTTCTCTTTGTAATCGGGAAATTACATTTAGCTGGTCCTTATTCAGTGAAAAGTACTAAAATTTGGGCCAATTGCTGCAAGGTGGTGAACCAGTCAGGATTGAACCTTCTATGTCACTTCTTTGACAAGCAAAGTGTAACAGTTTCCAAAAGGCAGTCTGCGTCCATTATTTTAAGAAGGTGCTCTGATTTGGAAAACAAACCTTCAGAAATTGCGTCAGTTGCATCAATCCAACTCGCTGATCCTAATAGATCTTTTATGACTAAATCAATTGAGGTCTCTCTTTTAGATGCTCGAGTACTTGCTTCGAGAACACGCATAACATCACTTCAGGATTCAAGAACTTACCCTGGGCCATTTGTTGTTGTTGATGTTTCAAGGAAGTCCGAGGATGGGTTGTCAATTGTTATTTCTCCTCTGACAAGAATACATAATGAAACCGGACTGCCTGTAAAGCTACGATTTCGACGAGCTCAGCAGAAGGAAGATGAGTTTGCTTCTGAGGTGCTAAATGTGGGTGATACAGTTGATGACTCCATGGCCATGTTTGGTGCTATAAATCTGTCTGGTGGAGAAAAAAAGGCACTGATGTCTTTAGCTGTTGGTGACTTCTTGTTGTCTTTTAGACCTGAAATTTCAGATGGGTTAATGAATTCCAAGAATCCACTCATAGTGGAATGGTCAGATGATCTTAAAGGTGGAAAGGCAGTCCGTCTCTCTGGAATTTTTGATAGATTAAGTTACAGAGTGCGGAATGCTTTGTTTACTGAATCGGTGAAATGTTCCTTCAGCACTGCATATTGTATCCTTAAATCTGAAGGGTCCAGTATTTCCAACATGCATTTTCTAGTTCAGAGTATTGGGAGAAGTGTTCCTGTTGTAGAACCTAATAGGTCTACGGATGGTCTTGAAAATAGCAAATTACCAGTTGCACTGCAAGAGCAAAAGGATATTTATCTTCTTCCTACCGTACGTGTATCCAATTTGTTGCACACTGAGATACATGTTTTCTTAAGTGAATCAGATCGATGTTCCACTACCGGGTCTGACAACAACAGAAATCAATCAACTGTGTCATGTGGTTCGACGGTTGACTTTTATGCGAATCCATCCATAATTTACTTTACTGTTACTTTGACTGCTTATAATACAAGCTGCAAACCAGTGAACAGCAGTGATTGGGTAAAAAAGTTACTGAAGCAGAAGAGTGATGTCCCTTGTTTGGACATTGATCTGGAATTTGGTGGGGGAAAACATTTTGCTTCCTTAAGATTGTCTCGAGGGAACAGGGGCACACTAGAGGCTGCTATTTTCACGTCATATTCCCTGAGAAATGATACAGAGTTTACCCTTTATTTCTTTGCCCCTAATAAGAAGCCTCTTTCTAGGGATGAAGTTGAGAACCATGGTTCTAGCATTCCTCCAGAGGTGGGATTATATTTGCCTCCAAAGTCAATTGGATCGTGGTTTTTACGACCCAACAAAGTGTCTCTCAAATTTCTGAAAGATAATGCATCAGAGACACTGATCGACCTGGATGCTTTATCGGGCCTTGCGGAAGTAAGCTTGGAAGTGGAGGACGGATCTGGAGTCAAATATATTACAAAGCTTGGGGTTTCCACGGGACCACCTTTGAGTAAAGTGGTCGTTCCATCACAAATAGTAACGATGGTCCCAAGGCATGTTGTAGTTAATGAATCAGAAGAAAGTATCCAAGTTCGTCAATGTTATTTACAGGATGACTCAGTAGGCATGATCCTCATAAACAGTAAGCAGAGGGCAGCTCTCCAGCTTCGGGATGGATTGAGCAAGAAGAGAGAGTTCGGTTTATTTGAGCATATTATGAAGAAGCACAGAAAAGTTAATGAAGATTCACTAATATATCTCCAGTTTCGGTTGCATGAGTCTGAATTAGGTTGGTCAGGACCAATATGCATTGCTTCTCTGGGAAGATTTTTCCTCAAATTCAAAAAGCCTTGTATGGACCAAGTGACAGCAGTAGAAAGCAATGTAACAGAATTTGCTGCTGTTCATGTTGTGGAAGAAGGTTCTTCTCTTGTGTTACGCTTCCATAAACCTCCAAACGTCAGTCTACCATATCGAATAGAGAATCGCTTGTGTGATGTATCTGTAACTTACTACCAGAAGGATTCATTGGAGCCTGAGATTCTTGGATCTGAAAGTGGTACTGATTATGTCTGGGATGATTCGACTCTCCCTCATAAGTTGGTTGTCAGAATCAATGGTATCTTCTACGTGAAATTAACTTGGATAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SHR-BD VPS13_C

Protein Analysis

2523

Amino Acids

281.8

Weight (kDa)

6.31

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_VAB PF25036 2080 - 2498 2.6e-22 Vacuolar-sorting associated protein 13, adaptor binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 6435
AarI CACCTGC 1 cut(s) 4094
Acc36I ACCTGC 1 cut(s) 4094
Acc65I GGTACC 1 cut(s) 3269
AccB1I GGYRCC 1 cut(s) 3269
AccB7I CCANNNNNTGG 1 cut(s) 4589
AccI GTMKAC 2 cut(s) 6185, 7381
AccII CGCG 3 cut(s) 22, 107, 5218
AccIII TCCGGA 1 cut(s) 796
AciI CCGC 8 cut(s) 15, 46, 107, 1763, 3546, 4427, 6040, 6845
AcoI YGGCCR 5 cut(s) 47, 165, 402, 2315, 5829
AcvI CACGTG 1 cut(s) 3301
AcyI GRCGYC 1 cut(s) 258
AdeI CACNNNGTG 1 cut(s) 3149
AfeI AGCGCT 1 cut(s) 4612
AflII CTTAAG 4 cut(s) 2575, 4132, 6301, 6556
AjiI CACGTC 2 cut(s) 5094, 6606
AjnI CCWGG 9 cut(s) 57, 113, 1268, 4027, 4582, 4789, 5078, 5638, 6822
AjuI GAANNNNNNNTTGG 6 cut(s) 1467, 1499, 1961, 1993, 7427, 7459
Alw44I GTGCAC 4 cut(s) 606, 718, 2291, 3296
AlwNI CAGNNNCTG 4 cut(s) 1931, 2363, 3689, 4589
Ama87I CYCGRG 3 cut(s) 362, 5583, 6567
Aor13HI TCCGGA 1 cut(s) 796
Aor51HI AGCGCT 1 cut(s) 4612
AoxI GGCC 8 cut(s) 47, 165, 402, 2315, 5355, 5642, 5829, 6838
ApaLI GTGCAC 4 cut(s) 606, 718, 2291, 3296
ArsI GACNNNNNNTTYG 2 cut(s) 5040, 5072
Asp700I GAANNNNTTC 4 cut(s) 3309, 3858, 4814, 4918
Asp718I GGTACC 1 cut(s) 3269
AspLEI GCGC 3 cut(s) 22, 4569, 4613
AsuC2I CCSGG 1 cut(s) 6331
AsuII TTCGAA 2 cut(s) 1619, 1940
AsuNHI GCTAGC 1 cut(s) 271
AvaI CYCGRG 3 cut(s) 362, 5583, 6567
AxyI CCTNAGG 3 cut(s) 2356, 2387, 4068
BaeGI GKGCMC 6 cut(s) 610, 722, 2295, 3300, 4633, 6584
BalI TGGCCA 3 cut(s) 404, 2317, 5831
BanI GGYRCC 1 cut(s) 3269
BanII GRGCYC 8 cut(s) 69, 142, 153, 635, 2833, 4465, 4692, 5764
BarI GAAGNNNNNNTAC 4 cut(s) 1122, 1154, 3262, 3294
BauI CACGAG 2 cut(s) 749, 4836
BbrPI CACGTG 1 cut(s) 3301
BbsI GAAGAC 4 cut(s) 578, 1560, 3498, 3837
BcgI CGANNNNNNTGC 4 cut(s) 353, 387, 6809, 6843
BciT130I CCWGG 9 cut(s) 59, 115, 1270, 4029, 4584, 4791, 5080, 5640, 6824
BciVI GTATCC 4 cut(s) 5272, 6104, 6277, 7010
BclI TGATCA 4 cut(s) 574, 1018, 1756, 2980
BcnI CCSGG 1 cut(s) 6331
BfmI CTRYAG 5 cut(s) 2307, 3352, 3681, 4830, 5239
BfoI RGCGCY 1 cut(s) 4614
BfrI CTTAAG 4 cut(s) 2575, 4132, 6301, 6556
BfuAI ACCTGC 1 cut(s) 4094
BfuI GTATCC 4 cut(s) 5272, 6104, 6277, 7010
BglII AGATCT 1 cut(s) 5540
BlpI GCTNAGC 1 cut(s) 5763
BmcAI AGTACT 3 cut(s) 1862, 5344, 5589
BmeT110I CYCGRG 3 cut(s) 362, 5583, 6567
BmgBI CACGTC 2 cut(s) 5094, 6606
BmrI ACTGGG 3 cut(s) 144, 4693, 5150
BmtI GCTAGC 1 cut(s) 275
BmuI ACTGGG 3 cut(s) 144, 4693, 5150
BoxI GACNNNNGTC 1 cut(s) 2055
BpiI GAAGAC 4 cut(s) 578, 1560, 3498, 3837
BplI GAGNNNNNCTC 4 cut(s) 5133, 5165, 7057, 7089
BpmI CTGGAG 7 cut(s) 41, 1291, 1392, 6696, 6897, 7061, 7160
Bpu1102I GCTNAGC 1 cut(s) 5763
Bpu14I TTCGAA 2 cut(s) 1619, 1940
BpuEI CTTGAG 4 cut(s) 223, 1226, 2867, 3884
BpuMI CCSGG 1 cut(s) 6331
Bsa29I ATCGAT 1 cut(s) 6316
BsaAI YACGTR 3 cut(s) 3301, 6263, 7546
BsaBI GATNNNNATC 4 cut(s) 759, 1609, 4374, 6243
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 5572
BsaWI WCCGGW 3 cut(s) 123, 796, 5729
BsaXI ACNNNNNCTCC 6 cut(s) 397, 427, 778, 808, 4400, 4430
Bse118I RCCGGY 1 cut(s) 1123
Bse21I CCTNAGG 3 cut(s) 2356, 2387, 4068
Bse3DI GCAATG 3 cut(s) 2724, 7224, 7303
Bse8I GATNNNNATC 4 cut(s) 759, 1609, 4374, 6243
BseAI TCCGGA 1 cut(s) 796
BseBI CCWGG 9 cut(s) 59, 115, 1270, 4029, 4584, 4791, 5080, 5640, 6824
BseCI ATCGAT 1 cut(s) 6316
BseJI GATNNNNATC 4 cut(s) 759, 1609, 4374, 6243
BseMI GCAATG 3 cut(s) 2724, 7224, 7303
BseRI GAGGAG 3 cut(s) 299, 4421, 5694
BseSI GKGCMC 6 cut(s) 610, 722, 2295, 3300, 4633, 6584
BseX3I CGGCCG 1 cut(s) 165
BsgI GTGCAG 3 cut(s) 3126, 4452, 5085
Bsh1236I CGCG 3 cut(s) 22, 107, 5218
Bsh1285I CGRYCG 2 cut(s) 111, 168
BshFI GGCC 8 cut(s) 49, 167, 404, 2317, 5357, 5644, 5831, 6840
BshNI GGYRCC 1 cut(s) 3269
BshVI ATCGAT 1 cut(s) 6316
BsiEI CGRYCG 2 cut(s) 111, 168
BsiHKCI CYCGRG 3 cut(s) 362, 5583, 6567
BsiSI CCGG 5 cut(s) 124, 797, 1124, 5730, 6330
BsiWI CGTACG 1 cut(s) 6259
BslFI GGGAC 6 cut(s) 215, 1971, 2650, 6488, 6927, 6947
BsmBI CGTCTC 2 cut(s) 4325, 6008
BsmFI GGGAC 6 cut(s) 215, 1971, 2650, 6488, 6927, 6947
BsmI GAATGC 3 cut(s) 4073, 6049, 6705
BsnI GGCC 8 cut(s) 49, 167, 404, 2317, 5357, 5644, 5831, 6840
Bso31I GGTCTC 1 cut(s) 5572
BsoBI CYCGRG 3 cut(s) 362, 5583, 6567
Bsp119I TTCGAA 2 cut(s) 1619, 1940
Bsp13I TCCGGA 1 cut(s) 796
Bsp1407I TGTACA 5 cut(s) 490, 785, 1138, 2821, 3749
Bsp1720I GCTNAGC 1 cut(s) 5763
Bsp19I CCATGG 4 cut(s) 50, 1956, 5826, 6694
Bsp68I TCGCGA 1 cut(s) 5218
BspACI CCGC 8 cut(s) 15, 46, 107, 1763, 3546, 4427, 6040, 6845
BspANI GGCC 8 cut(s) 49, 167, 404, 2317, 5357, 5644, 5831, 6840
BspDI ATCGAT 1 cut(s) 6316
BspEI TCCGGA 1 cut(s) 796
BspFNI CGCG 3 cut(s) 22, 107, 5218
BspHI TCATGA 1 cut(s) 2983
BspMAI CTGCAG 3 cut(s) 2311, 3685, 5243
BspMI ACCTGC 1 cut(s) 4094
BspOI GCTAGC 1 cut(s) 275
BspQI GCTCTTC 3 cut(s) 57, 158, 1332
BspT104I TTCGAA 2 cut(s) 1619, 1940
BspT107I GGYRCC 1 cut(s) 3269
BspTI CTTAAG 4 cut(s) 2575, 4132, 6301, 6556
BspTNI GGTCTC 1 cut(s) 5572
BsrDI GCAATG 3 cut(s) 2724, 7224, 7303
BsrFI RCCGGY 1 cut(s) 1123
BsrGI TGTACA 5 cut(s) 490, 785, 1138, 2821, 3749
BssAI RCCGGY 1 cut(s) 1123
BssNI GRCGYC 1 cut(s) 258
BssSI CACGAG 2 cut(s) 749, 4836
BssT1I CCWWGG 7 cut(s) 50, 841, 1956, 4861, 5826, 6694, 6965
Bst2BI CACGAG 2 cut(s) 749, 4836
Bst2UI CCWGG 9 cut(s) 59, 115, 1270, 4029, 4584, 4791, 5080, 5640, 6824
Bst6I CTCTTC 7 cut(s) 57, 158, 1025, 1332, 2927, 6488, 7097
BstACI GRCGYC 1 cut(s) 258
BstAFI CTTAAG 4 cut(s) 2575, 4132, 6301, 6556
BstAPI GCANNNNNTGC 1 cut(s) 1178
BstAUI TGTACA 5 cut(s) 490, 785, 1138, 2821, 3749
BstBAI YACGTR 3 cut(s) 3301, 6263, 7546
BstBI TTCGAA 2 cut(s) 1619, 1940
BstC8I GCNNGC 7 cut(s) 273, 649, 1125, 1710, 2111, 2700, 4101
BstDSI CCRYGG 5 cut(s) 50, 1956, 5826, 6694, 6909
BstEII GGTNACC 1 cut(s) 4255
BstENI CCTNNNNNAGG 2 cut(s) 4067, 4865
BstFNI CGCG 3 cut(s) 22, 107, 5218
BstH2I RGCGCY 1 cut(s) 4614
BstHHI GCGC 3 cut(s) 22, 4569, 4613
BstMCI CGRYCG 2 cut(s) 111, 168
BstNI CCWGG 9 cut(s) 59, 115, 1270, 4029, 4584, 4791, 5080, 5640, 6824
BstPAI GACNNNNGTC 1 cut(s) 2055
BstPI GGTNACC 1 cut(s) 4255
BstSFI CTRYAG 5 cut(s) 2307, 3352, 3681, 4830, 5239
BstSLI GKGCMC 6 cut(s) 610, 722, 2295, 3300, 4633, 6584
BstUI CGCG 3 cut(s) 22, 107, 5218
BstV2I GAAGAC 4 cut(s) 578, 1560, 3498, 3837
BstZI CGGCCG 1 cut(s) 165
Bsu15I ATCGAT 1 cut(s) 6316
Bsu36I CCTNAGG 3 cut(s) 2356, 2387, 4068
BsuI GTATCC 4 cut(s) 5272, 6104, 6277, 7010
BsuRI GGCC 8 cut(s) 49, 167, 404, 2317, 5357, 5644, 5831, 6840
BsuTUI ATCGAT 1 cut(s) 6316
BtgI CCRYGG 5 cut(s) 50, 1956, 5826, 6694, 6909
BtrI CACGTC 2 cut(s) 5094, 6606
BtsI GCAGTG 4 cut(s) 5236, 6081, 6221, 6469
BtuMI TCGCGA 1 cut(s) 5218
BveI ACCTGC 1 cut(s) 4094
Cac8I GCNNGC 7 cut(s) 273, 649, 1125, 1710, 2111, 2700, 4101
CaiI CAGNNNCTG 4 cut(s) 1931, 2363, 3689, 4589
CciI TCATGA 1 cut(s) 2983
CfoI GCGC 3 cut(s) 22, 4569, 4613
Cfr10I RCCGGY 1 cut(s) 1123
ClaI ATCGAT 1 cut(s) 6316
CseI GACGC 3 cut(s) 247, 5439, 5493
DraI TTTAAA 4 cut(s) 297, 430, 1203, 2422
DraIII CACNNNGTG 1 cut(s) 3149
EaeI YGGCCR 5 cut(s) 47, 165, 402, 2315, 5829
EagI CGGCCG 1 cut(s) 165
Eam1104I CTCTTC 7 cut(s) 57, 158, 1025, 1332, 2927, 6488, 7097
EarI CTCTTC 7 cut(s) 57, 158, 1025, 1332, 2927, 6488, 7097
Ecl136II GAGCTC 4 cut(s) 151, 633, 2831, 5762
EclXI CGGCCG 1 cut(s) 165
Eco130I CCWWGG 7 cut(s) 50, 841, 1956, 4861, 5826, 6694, 6965
Eco24I GRGCYC 8 cut(s) 69, 142, 153, 635, 2833, 4465, 4692, 5764
Eco31I GGTCTC 1 cut(s) 5572
Eco32I GATATC 6 cut(s) 484, 550, 2896, 3012, 4195, 4530
Eco47III AGCGCT 1 cut(s) 4612
Eco52I CGGCCG 1 cut(s) 165
Eco53kI GAGCTC 4 cut(s) 151, 633, 2831, 5762
Eco72I CACGTG 1 cut(s) 3301
Eco81I CCTNAGG 3 cut(s) 2356, 2387, 4068
Eco88I CYCGRG 3 cut(s) 362, 5583, 6567
Eco91I GGTNACC 1 cut(s) 4255
EcoICRI GAGCTC 4 cut(s) 151, 633, 2831, 5762
EcoNI CCTNNNNNAGG 2 cut(s) 4067, 4865
EcoO109I RGGNCCY 2 cut(s) 2871, 4025
EcoO65I GGTNACC 1 cut(s) 4255
EcoRI GAATTC 3 cut(s) 3549, 4313, 5941
EcoRII CCWGG 9 cut(s) 57, 113, 1268, 4027, 4582, 4789, 5078, 5638, 6822
EcoRV GATATC 6 cut(s) 484, 550, 2896, 3012, 4195, 4530
EcoT14I CCWWGG 7 cut(s) 50, 841, 1956, 4861, 5826, 6694, 6965
EcoT22I ATGCAT 4 cut(s) 5171, 6132, 6805, 7226
EcoT38I GRGCYC 8 cut(s) 69, 142, 153, 635, 2833, 4465, 4692, 5764
ErhI CCWWGG 7 cut(s) 50, 841, 1956, 4861, 5826, 6694, 6965
Esp3I CGTCTC 2 cut(s) 4325, 6008
FalI AAGNNNNNCTT 4 cut(s) 423, 455, 4126, 4158
FaqI GGGAC 6 cut(s) 215, 1971, 2650, 6488, 6927, 6947
FauI CCCGC 1 cut(s) 4420
FauNDI CATATG 1 cut(s) 3696
FbaI TGATCA 4 cut(s) 574, 1018, 1756, 2980
FblI GTMKAC 2 cut(s) 6185, 7381
FriOI GRGCYC 8 cut(s) 69, 142, 153, 635, 2833, 4465, 4692, 5764
GlaI GCGC 3 cut(s) 21, 4568, 4612
GsuI CTGGAG 7 cut(s) 41, 1291, 1392, 6696, 6897, 7061, 7160
HaeII RGCGCY 1 cut(s) 4614
HaeIII GGCC 8 cut(s) 49, 167, 404, 2317, 5357, 5644, 5831, 6840
HapII CCGG 5 cut(s) 124, 797, 1124, 5730, 6330
HgaI GACGC 3 cut(s) 247, 5439, 5493
HhaI GCGC 3 cut(s) 22, 4569, 4613
Hin1I GRCGYC 1 cut(s) 258
Hin6I GCGC 3 cut(s) 20, 4567, 4611
HinP1I GCGC 3 cut(s) 20, 4567, 4611
HincII GTYRAC 4 cut(s) 1314, 4600, 5097, 6382
HindII GTYRAC 4 cut(s) 1314, 4600, 5097, 6382
HindIII AAGCTT 8 cut(s) 344, 395, 1623, 3582, 3783, 4937, 6853, 6896
HpaI GTTAAC 1 cut(s) 1314
HpaII CCGG 5 cut(s) 124, 797, 1124, 5730, 6330
Hpy99I CGWCG 7 cut(s) 27, 161, 542, 3728, 4673, 5760, 6379
Hsp92I GRCGYC 1 cut(s) 258
HspAI GCGC 3 cut(s) 20, 4567, 4611
Kpn2I TCCGGA 1 cut(s) 796
KpnI GGTACC 1 cut(s) 3273
KroI GCCGGC 1 cut(s) 1123
KroNI GCCGGC 1 cut(s) 1125
Ksp22I TGATCA 4 cut(s) 574, 1018, 1756, 2980
KspAI GTTAAC 1 cut(s) 1314
LguI GCTCTTC 3 cut(s) 57, 158, 1332
LmnI GCTCC 8 cut(s) 60, 268, 2360, 2828, 2836, 4558, 4687, 7447
MfeI CAATTG 7 cut(s) 1002, 3890, 4700, 5359, 5559, 5691, 6744
MlsI TGGCCA 3 cut(s) 404, 2317, 5831
MluNI TGGCCA 3 cut(s) 404, 2317, 5831
MlyI GAGTC 9 cut(s) 1937, 2269, 2504, 4736, 5816, 6888, 7025, 7202, 7495
MmeI TCCRAC 6 cut(s) 440, 895, 2373, 4147, 5545, 6147
Mox20I TGGCCA 3 cut(s) 404, 2317, 5831
Mph1103I ATGCAT 4 cut(s) 5171, 6132, 6805, 7226
MroI TCCGGA 1 cut(s) 796
MroNI GCCGGC 1 cut(s) 1123
MroXI GAANNNNTTC 4 cut(s) 3309, 3858, 4814, 4918
MscI TGGCCA 3 cut(s) 404, 2317, 5831
MslI CAYNNNNRTG 6 cut(s) 1440, 2204, 2484, 3776, 4104, 5831
Msp20I TGGCCA 3 cut(s) 404, 2317, 5831
MspA1I CMGCKG 2 cut(s) 1040, 2189
MspCI CTTAAG 4 cut(s) 2575, 4132, 6301, 6556
MspI CCGG 5 cut(s) 124, 797, 1124, 5730, 6330
MunI CAATTG 7 cut(s) 1002, 3890, 4700, 5359, 5559, 5691, 6744
Mva1269I GAATGC 3 cut(s) 4073, 6049, 6705
MvaI CCWGG 9 cut(s) 59, 115, 1270, 4029, 4584, 4791, 5080, 5640, 6824
MvnI CGCG 3 cut(s) 22, 107, 5218
NaeI GCCGGC 1 cut(s) 1125
NciI CCSGG 1 cut(s) 6331
NcoI CCATGG 4 cut(s) 50, 1956, 5826, 6694
NdeI CATATG 1 cut(s) 3696
NgoMIV GCCGGC 1 cut(s) 1123
NheI GCTAGC 1 cut(s) 271
NmeAIII GCCGAG 1 cut(s) 193
NmuCI GTSAC 6 cut(s) 187, 2531, 4673, 5402, 5894, 7279
NruI TCGCGA 1 cut(s) 5218
NsiI ATGCAT 4 cut(s) 5171, 6132, 6805, 7226
NspV TTCGAA 2 cut(s) 1619, 1940
PaeI GCATGC 1 cut(s) 4103
PaeR7I CTCGAG 2 cut(s) 5583, 6567
PagI TCATGA 1 cut(s) 2983
PaqCI CACCTGC 1 cut(s) 4094
PasI CCCWGGG 1 cut(s) 5639
PciI ACATGT 7 cut(s) 1557, 1806, 2164, 4296, 4383, 5010, 6292
PciSI GCTCTTC 3 cut(s) 57, 158, 1332
PcsI WCGNNNNNNNCGW 4 cut(s) 153, 165, 4340, 5755
PctI GAATGC 3 cut(s) 4073, 6049, 6705
PdiI GCCGGC 1 cut(s) 1125
PdmI GAANNNNTTC 4 cut(s) 3309, 3858, 4814, 4918
Pfl23II CGTACG 1 cut(s) 6259
PflMI CCANNNNNTGG 1 cut(s) 4589
PfoI TCCNGGA 2 cut(s) 57, 4582
PleI GAGTC 9 cut(s) 1936, 2268, 2504, 4736, 5816, 6887, 7025, 7201, 7495
PmaCI CACGTG 1 cut(s) 3301
PmlI CACGTG 1 cut(s) 3301
PpsI GAGTC 9 cut(s) 1936, 2268, 2504, 4736, 5816, 6887, 7025, 7201, 7495
Ppu21I YACGTR 3 cut(s) 3301, 6263, 7546
PpuMI RGGWCCY 2 cut(s) 2871, 4025
PscI ACATGT 7 cut(s) 1557, 1806, 2164, 4296, 4383, 5010, 6292
PshAI GACNNNNGTC 1 cut(s) 2055
PsiI TTATAA 1 cut(s) 6435
Psp124BI GAGCTC 4 cut(s) 153, 635, 2833, 5764
Psp5II RGGWCCY 2 cut(s) 2871, 4025
Psp6I CCWGG 9 cut(s) 57, 113, 1268, 4027, 4582, 4789, 5078, 5638, 6822
PspCI CACGTG 1 cut(s) 3301
PspEI GGTNACC 1 cut(s) 4255
PspGI CCWGG 9 cut(s) 57, 113, 1268, 4027, 4582, 4789, 5078, 5638, 6822
PspLI CGTACG 1 cut(s) 6259
PspPPI RGGWCCY 2 cut(s) 2871, 4025
PspXI VCTCGAGB 1 cut(s) 5583
PsrI GAACNNNNNNTAC 4 cut(s) 1557, 1589, 5035, 5067
PstI CTGCAG 3 cut(s) 2311, 3685, 5243
PstNI CAGNNNCTG 4 cut(s) 1931, 2363, 3689, 4589
PvuII CAGCTG 2 cut(s) 1040, 2189
RruI TCGCGA 1 cut(s) 5218
RseI CAYNNNNRTG 6 cut(s) 1440, 2204, 2484, 3776, 4104, 5831
SacI GAGCTC 4 cut(s) 153, 635, 2833, 5764
SapI GCTCTTC 3 cut(s) 57, 158, 1332
ScaI AGTACT 3 cut(s) 1862, 5344, 5589
SchI GAGTC 9 cut(s) 1937, 2269, 2504, 4736, 5816, 6888, 7025, 7202, 7495
SfcI CTRYAG 5 cut(s) 2307, 3352, 3681, 4830, 5239
Sfr274I CTCGAG 2 cut(s) 5583, 6567
SfuI TTCGAA 2 cut(s) 1619, 1940
SlaI CTCGAG 2 cut(s) 5583, 6567
SmiI ATTTAAAT 1 cut(s) 2422
SmiMI CAYNNNNRTG 6 cut(s) 1440, 2204, 2484, 3776, 4104, 5831
SphI GCATGC 1 cut(s) 4103
SsiI CCGC 8 cut(s) 15, 46, 107, 1763, 3546, 4427, 6040, 6845
SspI AATATT 3 cut(s) 1693, 3388, 4969
SstI GAGCTC 4 cut(s) 153, 635, 2833, 5764
StyI CCWWGG 7 cut(s) 50, 841, 1956, 4861, 5826, 6694, 6965
SwaI ATTTAAAT 1 cut(s) 2422
TauI GCSGC 1 cut(s) 49
TseFI GTSAC 6 cut(s) 187, 2531, 4673, 5402, 5894, 7279
Tsp45I GTSAC 6 cut(s) 187, 2531, 4673, 5402, 5894, 7279
Van91I CCANNNNNTGG 1 cut(s) 4589
Vha464I CTTAAG 4 cut(s) 2575, 4132, 6301, 6556
VneI GTGCAC 4 cut(s) 606, 718, 2291, 3296
XagI CCTNNNNNAGG 2 cut(s) 4067, 4865
XbaI TCTAGA 2 cut(s) 920, 5107
XcmI CCANNNNNNNNNTGG 4 cut(s) 2559, 4267, 4699, 7085
XhoI CTCGAG 2 cut(s) 5583, 6567
XmiI GTMKAC 2 cut(s) 6185, 7381
XmnI GAANNNNTTC 4 cut(s) 3309, 3858, 4814, 4918
ZrmI AGTACT 3 cut(s) 1862, 5344, 5589
Zsp2I ATGCAT 4 cut(s) 5171, 6132, 6805, 7226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.