pycom02g12660

Prolyl oligopeptidase, N-terminal beta-propeller domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014832
Physical Location & Seq
Forward (+)
9251340 .. 9252605
1266 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 957 bp
ATGTGCTCGGAGCATGGTATACACGAAATCTTCGACCGTTCGATCATTGACTATTACATTTTTGGCGGCACAATCAATCCAATATATGTCGGCCACCACCAATGTAACCCCCTACCATGCGCCACCTACTTAAACCCCTCCGTCAACATTGCCGCCGTCTGCGTTGGGCCCATAAGCCGTCGTCGACGGCGTCACCAAGGCCGCCACGGCCGCCGCAGAAGCCCGAGCTTCAACTTCTACGACCACCCGTGGGAGGACTCCTACAGCTGGATGTCGAAGCTCAACGACAAAGTGGCGTTGCGCCACCTGGACATGTACATGGAGCAAGAGGAGAAGTACACGGAGGCCGTCATGTCCGGCACCGACCGCCTTCAGAGCAAGCTCCAGGCCGAGATGGCCTCTCGCATCGCCTCCGACCTCTCCACCCCTCCCCTGCGCTGGGGCCCCTGGTTCTATTACCGGCGCGTGGAGGAGGGGAAGCAACTTCCGGTGCTCTGCCGGAGACTCGCGAGTTTAAACGACGAGTTCATTTCGCACAAATCGCCTTCCTCCGGATTCGATTTCACATCGGGAAAGGGGATCGATCAGAAGCTCATTGATTACAATCAGGAGTCCCAAAGATGTGGGAGTAAATTGATATGCTTACGAAGATCGGAAGTGTCGCCGGATCGTGGGTTTATAGCTTACACAATTTATGATAAGGAGGCTGACTTGTTTAGATTATCTGTTAGGAATTTGAACTCTGGTGCATTGTGTAGTAAGCCGAAAGTAGAGTGTGTTTCGAATTTGGCTTGGGCCAAAGACGGGCAGGCGTTGCTTTATGTTGTCACTGATGAAAACAGGCGGCCATATAGGCTAGTATTATGCGAAATTGTGCTGTTTATGCATGCTATTCTGTTATACTTGAGTGAAATGTTGGTAATTCTAGCCTATTTGGTCAATCAGGATATATTGTAG

Protein Analysis

319

Amino Acids

36.72

Weight (kDa)

8.46

Isoelectric Point (pI)

70.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9_N PF02897 78 - 285 5.5e-23 Prolyl oligopeptidase, N-terminal beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 359
AccI GTMKAC 2 cut(s) 19, 184
AccII CGCG 2 cut(s) 465, 509
AccIII TCCGGA 1 cut(s) 551
AciI CCGC 7 cut(s) 66, 153, 202, 211, 214, 367, 844
AclWI GGATC 2 cut(s) 587, 675
AcoI YGGCCR 3 cut(s) 91, 208, 845
AcsI RAATTY 2 cut(s) 733, 784
AcuI CTGAAG 1 cut(s) 356
AcyI GRCGYC 1 cut(s) 190
AfaI GTAC 2 cut(s) 317, 338
AfiI CCNNNNNNNGG 9 cut(s) 250, 253, 267, 438, 439, 466, 551, 671, 804
AflIII ACRYGT 1 cut(s) 312
AgsI TTSAA 2 cut(s) 232, 739
AjnI CCWGG 3 cut(s) 306, 384, 446
AluBI AGCT 6 cut(s) 228, 267, 280, 382, 592, 683
AluI AGCT 6 cut(s) 228, 267, 280, 382, 592, 683
Alw21I GWGCWC 2 cut(s) 8, 495
Alw26I GTCTC 1 cut(s) 496
AlwI GGATC 2 cut(s) 587, 675
Ama87I CYCGRG 1 cut(s) 223
Aor13HI TCCGGA 1 cut(s) 551
ApaI GGGCCC 2 cut(s) 171, 446
ApoI RAATTY 2 cut(s) 733, 784
AspLEI GCGC 4 cut(s) 122, 303, 438, 465
AspS9I GGNCC 5 cut(s) 167, 168, 442, 443, 795
AsuHPI GGTGA 1 cut(s) 185
AsuII TTCGAA 1 cut(s) 782
AvaI CYCGRG 1 cut(s) 223
BaeGI GKGCMC 2 cut(s) 171, 446
BanI GGYRCC 1 cut(s) 359
BanII GRGCYC 2 cut(s) 171, 446
Bbv12I GWGCWC 2 cut(s) 8, 495
BccI CCATC 1 cut(s) 388
BceAI ACGGC 5 cut(s) 140, 162, 203, 223, 332
BciT130I CCWGG 3 cut(s) 308, 386, 448
BcoDI GTCTC 1 cut(s) 496
BfaI CTAG 2 cut(s) 857, 926
BfmI CTRYAG 1 cut(s) 262
BglI GCCNNNNNGGC 3 cut(s) 207, 395, 853
BisI GCNGC 6 cut(s) 67, 153, 202, 211, 214, 845
BlsI GCNGC 6 cut(s) 68, 154, 203, 212, 215, 846
Bme1390I CCNGG 3 cut(s) 308, 386, 448
BmeT110I CYCGRG 1 cut(s) 223
BmgT120I GGNCC 5 cut(s) 167, 168, 442, 443, 795
BmiI GGNNCC 5 cut(s) 169, 361, 443, 444, 445
BmrFI CCNGG 3 cut(s) 308, 386, 448
BmsI GCATC 1 cut(s) 414
BplI GAGNNNNNCTC 2 cut(s) 383, 415
BpmI CTGGAG 1 cut(s) 368
Bpu14I TTCGAA 1 cut(s) 782
BpuEI CTTGAG 1 cut(s) 925
Bsa29I ATCGAT 1 cut(s) 582
BsaBI GATNNNNATC 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 190
BsaJI CCNNGG 4 cut(s) 196, 205, 248, 446
BsaWI WCCGGW 2 cut(s) 487, 551
Bsc4I CCNNNNNNNGG 9 cut(s) 250, 253, 267, 438, 439, 466, 551, 671, 804
Bse118I RCCGGY 1 cut(s) 459
Bse3DI GCAATG 1 cut(s) 147
Bse8I GATNNNNATC 1 cut(s) 603
BseAI TCCGGA 1 cut(s) 551
BseBI CCWGG 3 cut(s) 308, 386, 448
BseCI ATCGAT 1 cut(s) 582
BseDI CCNNGG 4 cut(s) 196, 205, 248, 446
BseGI GGATG 1 cut(s) 276
BseJI GATNNNNATC 1 cut(s) 603
BseLI CCNNNNNNNGG 9 cut(s) 250, 253, 267, 438, 439, 466, 551, 671, 804
BseMI GCAATG 1 cut(s) 147
BseRI GAGGAG 2 cut(s) 344, 485
BseSI GKGCMC 2 cut(s) 171, 446
BseX3I CGGCCG 1 cut(s) 208
BseYI CCCAGC 1 cut(s) 438
Bsh1236I CGCG 2 cut(s) 465, 509
Bsh1285I CGRYCG 3 cut(s) 37, 211, 367
BshNI GGYRCC 1 cut(s) 359
BshVI ATCGAT 1 cut(s) 582
BsiEI CGRYCG 3 cut(s) 37, 211, 367
BsiHKAI GWGCWC 2 cut(s) 8, 495
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 6 cut(s) 357, 460, 488, 499, 552, 665
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 9 cut(s) 250, 253, 267, 438, 439, 466, 551, 671, 804
BsmAI GTCTC 1 cut(s) 496
BsmFI GGGAC 1 cut(s) 598
BsoBI CYCGRG 1 cut(s) 223
Bsp119I TTCGAA 1 cut(s) 782
Bsp120I GGGCCC 2 cut(s) 167, 442
Bsp1286I GDGCHC 4 cut(s) 8, 171, 446, 495
Bsp13I TCCGGA 1 cut(s) 551
Bsp1407I TGTACA 1 cut(s) 315
Bsp143I GATC 5 cut(s) 42, 579, 583, 650, 667
Bsp68I TCGCGA 1 cut(s) 509
BspACI CCGC 7 cut(s) 66, 153, 202, 211, 214, 367, 844
BspDI ATCGAT 1 cut(s) 582
BspEI TCCGGA 1 cut(s) 551
BspFNI CGCG 2 cut(s) 465, 509
BspLI GGNNCC 5 cut(s) 169, 361, 443, 444, 445
BspPI GGATC 2 cut(s) 587, 675
BspT104I TTCGAA 1 cut(s) 782
BspT107I GGYRCC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 147
BsrFI RCCGGY 1 cut(s) 459
BsrGI TGTACA 1 cut(s) 315
BssAI RCCGGY 1 cut(s) 459
BssECI CCNNGG 4 cut(s) 196, 205, 248, 446
BssMI GATC 5 cut(s) 42, 579, 583, 650, 667
BssNAI GTATAC 1 cut(s) 20
BssNI GRCGYC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 196
Bst1107I GTATAC 1 cut(s) 20
Bst2UI CCWGG 3 cut(s) 308, 386, 448
Bst4CI ACNGT 1 cut(s) 38
BstACI GRCGYC 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 814
BstAUI TGTACA 1 cut(s) 315
BstBI TTCGAA 1 cut(s) 782
BstC8I GCNNGC 3 cut(s) 380, 810, 888
BstDSI CCRYGG 2 cut(s) 205, 248
BstF5I GGATG 1 cut(s) 276
BstFNI CGCG 2 cut(s) 465, 509
BstHHI GCGC 4 cut(s) 122, 303, 438, 465
BstKTI GATC 5 cut(s) 45, 582, 586, 653, 670
BstMAI GTCTC 1 cut(s) 496
BstMBI GATC 5 cut(s) 42, 579, 583, 650, 667
BstMCI CGRYCG 3 cut(s) 37, 211, 367
BstNI CCWGG 3 cut(s) 308, 386, 448
BstNSI RCATGY 2 cut(s) 316, 890
BstSCI CCNGG 3 cut(s) 306, 384, 446
BstSFI CTRYAG 1 cut(s) 262
BstSLI GKGCMC 2 cut(s) 171, 446
BstUI CGCG 2 cut(s) 465, 509
BstXI CCANNNNNNTGG 1 cut(s) 623
BstZ17I GTATAC 1 cut(s) 20
BstZI CGGCCG 1 cut(s) 208
Bsu15I ATCGAT 1 cut(s) 582
BsuTUI ATCGAT 1 cut(s) 582
BtgI CCRYGG 2 cut(s) 205, 248
BtgZI GCGATG 1 cut(s) 391
BtsCI GGATG 1 cut(s) 276
BtsIMutI CAGTG 1 cut(s) 828
BtuMI TCGCGA 1 cut(s) 509
Cac8I GCNNGC 3 cut(s) 380, 810, 888
CfoI GCGC 4 cut(s) 122, 303, 438, 465
Cfr10I RCCGGY 1 cut(s) 459
Cfr13I GGNCC 5 cut(s) 167, 168, 442, 443, 795
ClaI ATCGAT 1 cut(s) 582
CseI GACGC 1 cut(s) 179
Csp6I GTAC 2 cut(s) 316, 337
CviAII CATG 6 cut(s) 14, 117, 313, 319, 352, 887
CviQI GTAC 2 cut(s) 316, 337
DpnI GATC 5 cut(s) 44, 581, 585, 652, 669
DpnII GATC 5 cut(s) 42, 579, 583, 650, 667
DraI TTTAAA 1 cut(s) 516
EaeI YGGCCR 3 cut(s) 91, 208, 845
EagI CGGCCG 1 cut(s) 208
EclXI CGGCCG 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 196
Eco24I GRGCYC 2 cut(s) 171, 446
Eco52I CGGCCG 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 356
Eco88I CYCGRG 1 cut(s) 223
EcoO109I RGGNCCY 2 cut(s) 442, 443
EcoRII CCWGG 3 cut(s) 306, 384, 446
EcoT14I CCWWGG 1 cut(s) 196
EcoT22I ATGCAT 1 cut(s) 888
EcoT38I GRGCYC 2 cut(s) 171, 446
ErhI CCWWGG 1 cut(s) 196
FaeI CATG 6 cut(s) 17, 120, 316, 322, 355, 890
FaqI GGGAC 1 cut(s) 598
FatI CATG 6 cut(s) 13, 116, 312, 318, 351, 886
FblI GTMKAC 2 cut(s) 19, 184
Fnu4HI GCNGC 6 cut(s) 67, 153, 202, 211, 214, 845
FokI GGATG 1 cut(s) 283
FriOI GRGCYC 2 cut(s) 171, 446
Fsp4HI GCNGC 6 cut(s) 67, 153, 202, 211, 214, 845
FspBI CTAG 2 cut(s) 857, 926
GlaI GCGC 4 cut(s) 121, 302, 437, 464
GluI GCNGC 6 cut(s) 67, 153, 202, 211, 214, 845
GsaI CCCAGC 1 cut(s) 442
GsuI CTGGAG 1 cut(s) 368
HapII CCGG 6 cut(s) 357, 460, 488, 499, 552, 665
HgaI GACGC 1 cut(s) 179
HhaI GCGC 4 cut(s) 122, 303, 438, 465
Hin1I GRCGYC 1 cut(s) 190
Hin1II CATG 6 cut(s) 17, 120, 316, 322, 355, 890
Hin6I GCGC 4 cut(s) 120, 301, 436, 463
HinP1I GCGC 4 cut(s) 120, 301, 436, 463
HincII GTYRAC 2 cut(s) 145, 185
HindII GTYRAC 2 cut(s) 145, 185
HinfI GANTC 4 cut(s) 257, 504, 555, 611
HpaII CCGG 6 cut(s) 357, 460, 488, 499, 552, 665
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 4 cut(s) 20, 145, 185, 339
Hpy188I TCNGA 5 cut(s) 10, 375, 415, 588, 655
Hpy188III TCNNGA 5 cut(s) 508, 552, 570, 608, 944
Hpy8I GTNNAC 4 cut(s) 20, 145, 185, 339
Hpy99I CGWCG 4 cut(s) 183, 186, 189, 524
HpyAV CCTTC 2 cut(s) 380, 555
HpyCH4III ACNGT 1 cut(s) 38
HpyCH4V TGCA 2 cut(s) 749, 886
Hsp92I GRCGYC 1 cut(s) 190
Hsp92II CATG 6 cut(s) 17, 120, 316, 322, 355, 890
HspAI GCGC 4 cut(s) 120, 301, 436, 463
Kpn2I TCCGGA 1 cut(s) 551
Kzo9I GATC 5 cut(s) 42, 579, 583, 650, 667
LmnI GCTCC 3 cut(s) 10, 322, 387
LweI GCATC 1 cut(s) 414
MaeI CTAG 2 cut(s) 857, 926
MaeIII GTNAC 3 cut(s) 104, 191, 826
MalI GATC 5 cut(s) 44, 581, 585, 652, 669
MboI GATC 5 cut(s) 42, 579, 583, 650, 667
MboII GAAGA 2 cut(s) 22, 660
MhlI GDGCHC 4 cut(s) 8, 171, 446, 495
MluCI AATT 6 cut(s) 632, 690, 733, 784, 870, 921
MlyI GAGTC 3 cut(s) 251, 498, 620
MmeI TCCRAC 1 cut(s) 438
Mph1103I ATGCAT 1 cut(s) 888
MroI TCCGGA 1 cut(s) 551
MseI TTAA 2 cut(s) 131, 515
MslI CAYNNNNRTG 1 cut(s) 317
MspA1I CMGCKG 1 cut(s) 267
MspI CCGG 6 cut(s) 357, 460, 488, 499, 552, 665
MspR9I CCNGG 3 cut(s) 308, 386, 448
MssI GTTTAAAC 1 cut(s) 516
MvaI CCWGG 3 cut(s) 308, 386, 448
MvnI CGCG 2 cut(s) 465, 509
NdeII GATC 5 cut(s) 42, 579, 583, 650, 667
NlaIII CATG 6 cut(s) 17, 120, 316, 322, 355, 890
NlaIV GGNNCC 5 cut(s) 169, 361, 443, 444, 445
NmeAIII GCCGAG 1 cut(s) 415
NmuCI GTSAC 2 cut(s) 191, 826
NruI TCGCGA 1 cut(s) 509
NsiI ATGCAT 1 cut(s) 888
NspI RCATGY 2 cut(s) 316, 890
NspV TTCGAA 1 cut(s) 782
PaeI GCATGC 1 cut(s) 890
PciI ACATGT 1 cut(s) 312
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 187
PfeI GAWTC 1 cut(s) 555
PflFI GACNNNGTC 1 cut(s) 189
PkrI GCNGC 6 cut(s) 68, 154, 203, 212, 215, 846
PleI GAGTC 3 cut(s) 251, 498, 619
PmeI GTTTAAAC 1 cut(s) 516
PpsI GAGTC 3 cut(s) 251, 498, 619
PscI ACATGT 1 cut(s) 312
Psp6I CCWGG 3 cut(s) 306, 384, 446
PspFI CCCAGC 1 cut(s) 438
PspGI CCWGG 3 cut(s) 306, 384, 446
PspN4I GGNNCC 5 cut(s) 169, 361, 443, 444, 445
PspOMI GGGCCC 2 cut(s) 167, 442
PspPI GGNCC 5 cut(s) 167, 168, 442, 443, 795
PsyI GACNNNGTC 1 cut(s) 189
PvuII CAGCTG 1 cut(s) 267
RruI TCGCGA 1 cut(s) 509
RsaI GTAC 2 cut(s) 317, 338
RsaNI GTAC 2 cut(s) 316, 337
RseI CAYNNNNRTG 1 cut(s) 317
SalI GTCGAC 1 cut(s) 183
SaqAI TTAA 2 cut(s) 131, 515
SatI GCNGC 6 cut(s) 67, 153, 202, 211, 214, 845
Sau3AI GATC 5 cut(s) 42, 579, 583, 650, 667
Sau96I GGNCC 5 cut(s) 167, 168, 442, 443, 795
SchI GAGTC 3 cut(s) 251, 498, 620
ScrFI CCNGG 3 cut(s) 308, 386, 448
SduI GDGCHC 4 cut(s) 8, 171, 446, 495
SetI ASST 9 cut(s) 128, 230, 269, 282, 309, 384, 420, 594, 685
SfaNI GCATC 1 cut(s) 414
SfcI CTRYAG 1 cut(s) 262
SfiI GGCCNNNNNGGCC 2 cut(s) 207, 395
SfuI TTCGAA 1 cut(s) 782
SgrDI CGTCGACG 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 317
SmlI CTYRAG 1 cut(s) 904
SmoI CTYRAG 1 cut(s) 904
SphI GCATGC 1 cut(s) 890
Sse9I AATT 6 cut(s) 632, 690, 733, 784, 870, 921
SsiI CCGC 7 cut(s) 66, 153, 202, 211, 214, 367, 844
SspMI CTAG 2 cut(s) 857, 926
StyD4I CCNGG 3 cut(s) 306, 384, 446
StyI CCWWGG 1 cut(s) 196
TaaI ACNGT 1 cut(s) 38
TaqI TCGA 7 cut(s) 33, 41, 184, 275, 558, 582, 782
TasI AATT 6 cut(s) 632, 690, 733, 784, 870, 921
TatI WGTACW 2 cut(s) 315, 336
TauI GCSGC 6 cut(s) 69, 155, 204, 213, 216, 847
TfiI GAWTC 1 cut(s) 555
Tru1I TTAA 2 cut(s) 131, 515
Tru9I TTAA 2 cut(s) 131, 515
TscAI CASTG 1 cut(s) 835
TseFI GTSAC 2 cut(s) 191, 826
Tsp45I GTSAC 2 cut(s) 191, 826
TspDTI ATGAA 2 cut(s) 517, 849
TspGWI ACGGA 2 cut(s) 130, 356
TspRI CASTG 1 cut(s) 835
Tth111I GACNNNGTC 1 cut(s) 189
XapI RAATTY 2 cut(s) 733, 784
XceI RCATGY 2 cut(s) 316, 890
XmiI GTMKAC 2 cut(s) 19, 184
XspI CTAG 2 cut(s) 857, 926
Zsp2I ATGCAT 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.