Rh1CG039300

Prolyl oligopeptidase, N-terminal beta-propeller domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0014832
Physical Location & Seq
Reverse (-)
7519481 .. 7525077
5597 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG039300.1

Sequence Viewer

Length: 651 bp
ATGCTCTACCCTGTAAAACCCCTCCGCCCCCATCTCCGCCATCTACGGCTGGCCCACTTCAAGGCCCCAAAACCGCCGGAATGCCCTCCGAAGCCGCAAAACCCTCTCCAGAAGCCCGAAACCTTTACCCTCCATGACCACACGTGGCAGGACCCCTACAGATGGATGTTCAAACTCAACGACAAGGTCGCCATGCGACACATGGACATGTACATGGAGCAGGAAGACAAGTACACCAAAGCTGTCATGTACGGCTCCGACCGCCTCCAGAGCAGGCTCCAGTCCGAGATTGCCTCTCGCTTATCCTTCGACCTCTCCACTCCTCCCCTCAAGTTCGGACCATGTAAGCCTCAAGCGAAGCGTGTTTCGAATTTGTCTTGGGCCAAAGACGGGCAGGCATTGCTTTATGTTGTCACCAATCAGAACAAGCGGCCATATAGATGTTTTCTCGAGAAGGATGTTTTTGAAGAATCGGATAATAATGTGTTTGTCAACATTAGACACACGAAAGATTTCCGGTTTATGACTGTAAATACCTTCTCTACCGCGTCTTCCAAGATTTCAAGGACAAGGCGACTGTGGCAGATTGGACTGCAGTGCACTATGCAACTGAGGCAGCAGTGGAGGGAACCAAAGCAGCTGCAAGAGTAG

Protein Analysis

216

Amino Acids

25.8

Weight (kDa)

9.85

Isoelectric Point (pI)

62.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9_N PF02897 40 - 111 2.6e-06 Prolyl oligopeptidase, N-terminal beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 548
AciI CCGC 7 cut(s) 25, 37, 74, 95, 262, 430, 546
AcoI YGGCCR 1 cut(s) 431
AcsI RAATTY 1 cut(s) 370
AcvI CACGTG 1 cut(s) 144
AfaI GTAC 3 cut(s) 212, 233, 251
AfiI CCNNNNNNNGG 3 cut(s) 61, 162, 390
AflIII ACRYGT 2 cut(s) 141, 207
AgsI TTSAA 4 cut(s) 61, 172, 467, 564
AluBI AGCT 2 cut(s) 242, 640
AluI AGCT 2 cut(s) 242, 640
Alw21I GWGCWC 1 cut(s) 602
Alw44I GTGCAC 1 cut(s) 598
Ama87I CYCGRG 1 cut(s) 449
AoxI GGCC 4 cut(s) 51, 63, 381, 431
ApaLI GTGCAC 1 cut(s) 598
ApeKI GCWGC 3 cut(s) 616, 637, 640
ApoI RAATTY 1 cut(s) 370
Asp700I GAANNNNTTC 1 cut(s) 512
AspS9I GGNCC 5 cut(s) 52, 64, 151, 338, 381
AsuHPI GGTGA 1 cut(s) 406
AsuII TTCGAA 1 cut(s) 368
AvaI CYCGRG 1 cut(s) 449
AvaII GGWCC 2 cut(s) 151, 338
BaeGI GKGCMC 1 cut(s) 602
BbrPI CACGTG 1 cut(s) 144
BbsI GAAGAC 2 cut(s) 231, 543
Bbv12I GWGCWC 1 cut(s) 602
BbvI GCAGC 2 cut(s) 627, 628
BccI CCATC 3 cut(s) 39, 48, 156
BceAI ACGGC 2 cut(s) 62, 268
BfmI CTRYAG 2 cut(s) 157, 593
BisI GCNGC 5 cut(s) 95, 431, 617, 638, 641
BlsI GCNGC 5 cut(s) 96, 432, 618, 639, 642
Bme18I GGWCC 2 cut(s) 151, 338
BmeT110I CYCGRG 1 cut(s) 449
BmgT120I GGNCC 5 cut(s) 52, 64, 151, 338, 381
BmiI GGNNCC 5 cut(s) 66, 153, 256, 278, 630
BpiI GAAGAC 2 cut(s) 231, 543
BplI GAGNNNNNCTC 2 cut(s) 278, 310
BpmI CTGGAG 3 cut(s) 92, 251, 263
Bpu14I TTCGAA 1 cut(s) 368
BpuEI CTTGAG 2 cut(s) 314, 336
BsaAI YACGTR 1 cut(s) 144
BsaWI WCCGGW 1 cut(s) 516
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 162, 390
Bse1I ACTGG 1 cut(s) 280
Bse3DI GCAATG 1 cut(s) 398
BseGI GGATG 2 cut(s) 171, 463
BseLI CCNNNNNNNGG 3 cut(s) 61, 162, 390
BseMI GCAATG 1 cut(s) 398
BseMII CTCAG 1 cut(s) 602
BseNI ACTGG 1 cut(s) 280
BseRI GAGGAG 1 cut(s) 312
BseSI GKGCMC 1 cut(s) 602
BseXI GCAGC 2 cut(s) 627, 628
Bsh1236I CGCG 1 cut(s) 548
Bsh1285I CGRYCG 1 cut(s) 262
BshFI GGCC 4 cut(s) 53, 65, 383, 433
BsiEI CGRYCG 1 cut(s) 262
BsiHKAI GWGCWC 1 cut(s) 602
BsiHKCI CYCGRG 1 cut(s) 449
BsiSI CCGG 2 cut(s) 77, 517
BslI CCNNNNNNNGG 3 cut(s) 61, 162, 390
BsmI GAATGC 1 cut(s) 86
BsnI GGCC 4 cut(s) 53, 65, 383, 433
BsoBI CYCGRG 1 cut(s) 449
Bsp119I TTCGAA 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 602
Bsp1407I TGTACA 1 cut(s) 210
BspACI CCGC 7 cut(s) 25, 37, 74, 95, 262, 430, 546
BspANI GGCC 4 cut(s) 53, 65, 383, 433
BspCNI CTCAG 1 cut(s) 603
BspFNI CGCG 1 cut(s) 548
BspLI GGNNCC 5 cut(s) 66, 153, 256, 278, 630
BspMAI CTGCAG 1 cut(s) 597
BspT104I TTCGAA 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 398
BsrGI TGTACA 1 cut(s) 210
BsrI ACTGG 1 cut(s) 280
Bst4CI ACNGT 2 cut(s) 529, 579
BstAPI GCANNNNNTGC 1 cut(s) 400
BstAUI TGTACA 1 cut(s) 210
BstBAI YACGTR 1 cut(s) 144
BstBI TTCGAA 1 cut(s) 368
BstC8I GCNNGC 3 cut(s) 51, 275, 396
BstDEI CTNAG 1 cut(s) 611
BstF5I GGATG 2 cut(s) 171, 463
BstFNI CGCG 1 cut(s) 548
BstMCI CGRYCG 1 cut(s) 262
BstMWI GCNNNNNNNGC 5 cut(s) 261, 270, 400, 580, 613
BstNSI RCATGY 1 cut(s) 211
BstSFI CTRYAG 2 cut(s) 157, 593
BstSLI GKGCMC 1 cut(s) 602
BstUI CGCG 1 cut(s) 548
BstV1I GCAGC 2 cut(s) 627, 628
BstV2I GAAGAC 2 cut(s) 231, 543
BsuRI GGCC 4 cut(s) 53, 65, 383, 433
BtsCI GGATG 2 cut(s) 171, 463
BtsI GCAGTG 2 cut(s) 602, 626
BtsIMutI CAGTG 2 cut(s) 602, 626
Cac8I GCNNGC 3 cut(s) 51, 275, 396
Cfr13I GGNCC 5 cut(s) 52, 64, 151, 338, 381
CseI GACGC 1 cut(s) 537
Csp6I GTAC 3 cut(s) 211, 232, 250
CviAII CATG 7 cut(s) 134, 193, 202, 208, 214, 247, 342
CviQI GTAC 3 cut(s) 211, 232, 250
DdeI CTNAG 1 cut(s) 611
EaeI YGGCCR 1 cut(s) 431
EciI GGCGGA 2 cut(s) 14, 26
Eco47I GGWCC 2 cut(s) 151, 338
Eco72I CACGTG 1 cut(s) 144
Eco88I CYCGRG 1 cut(s) 449
EcoO109I RGGNCCY 2 cut(s) 64, 151
FaeI CATG 7 cut(s) 137, 196, 205, 211, 217, 250, 345
FatI CATG 7 cut(s) 133, 192, 201, 207, 213, 246, 341
Fnu4HI GCNGC 5 cut(s) 95, 431, 617, 638, 641
FokI GGATG 2 cut(s) 178, 470
Fsp4HI GCNGC 5 cut(s) 95, 431, 617, 638, 641
GluI GCNGC 5 cut(s) 95, 431, 617, 638, 641
GsuI CTGGAG 3 cut(s) 92, 251, 263
HaeIII GGCC 4 cut(s) 53, 65, 383, 433
HapII CCGG 2 cut(s) 77, 517
HgaI GACGC 1 cut(s) 537
Hin1II CATG 7 cut(s) 137, 196, 205, 211, 217, 250, 345
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HinfI GANTC 1 cut(s) 470
HpaII CCGG 2 cut(s) 77, 517
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 3 cut(s) 234, 493, 600
Hpy188I TCNGA 6 cut(s) 90, 259, 286, 338, 423, 475
Hpy188III TCNNGA 4 cut(s) 109, 268, 449, 451
Hpy8I GTNNAC 3 cut(s) 234, 493, 600
HpyAV CCTTC 3 cut(s) 316, 448, 547
HpyCH4III ACNGT 2 cut(s) 529, 579
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 4 cut(s) 595, 600, 607, 643
HpyF10VI GCNNNNNNNGC 5 cut(s) 261, 270, 400, 580, 613
HpyF3I CTNAG 1 cut(s) 611
HpySE526I ACGT 1 cut(s) 143
Hsp92II CATG 7 cut(s) 137, 196, 205, 211, 217, 250, 345
LmnI GCTCC 3 cut(s) 217, 260, 282
Lsp1109I GCAGC 2 cut(s) 627, 628
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 1 cut(s) 412
MboII GAAGA 3 cut(s) 236, 479, 543
MhlI GDGCHC 1 cut(s) 602
MluCI AATT 1 cut(s) 370
MmeI TCCRAC 1 cut(s) 282
MroXI GAANNNNTTC 1 cut(s) 512
MslI CAYNNNNRTG 3 cut(s) 206, 212, 439
MspA1I CMGCKG 1 cut(s) 640
MspI CCGG 2 cut(s) 77, 517
Mva1269I GAATGC 1 cut(s) 86
MvnI CGCG 1 cut(s) 548
MwoI GCNNNNNNNGC 5 cut(s) 261, 270, 400, 580, 613
NlaIII CATG 7 cut(s) 137, 196, 205, 211, 217, 250, 345
NlaIV GGNNCC 5 cut(s) 66, 153, 256, 278, 630
NmuCI GTSAC 1 cut(s) 412
NspI RCATGY 1 cut(s) 211
NspV TTCGAA 1 cut(s) 368
PaeR7I CTCGAG 1 cut(s) 449
PciI ACATGT 1 cut(s) 207
PctI GAATGC 1 cut(s) 86
PdmI GAANNNNTTC 1 cut(s) 512
PfeI GAWTC 1 cut(s) 470
PflFI GACNNNGTC 1 cut(s) 185
PkrI GCNGC 5 cut(s) 96, 432, 618, 639, 642
PmaCI CACGTG 1 cut(s) 144
PmlI CACGTG 1 cut(s) 144
Ppu21I YACGTR 1 cut(s) 144
PpuMI RGGWCCY 1 cut(s) 151
PscI ACATGT 1 cut(s) 207
Psp5II RGGWCCY 1 cut(s) 151
PspCI CACGTG 1 cut(s) 144
PspN4I GGNNCC 5 cut(s) 66, 153, 256, 278, 630
PspPI GGNCC 5 cut(s) 52, 64, 151, 338, 381
PspPPI RGGWCCY 1 cut(s) 151
PstI CTGCAG 1 cut(s) 597
PsyI GACNNNGTC 1 cut(s) 185
PvuII CAGCTG 1 cut(s) 640
RsaI GTAC 3 cut(s) 212, 233, 251
RsaNI GTAC 3 cut(s) 211, 232, 250
RseI CAYNNNNRTG 3 cut(s) 206, 212, 439
SatI GCNGC 5 cut(s) 95, 431, 617, 638, 641
Sau96I GGNCC 5 cut(s) 52, 64, 151, 338, 381
SduI GDGCHC 1 cut(s) 602
SetI ASST 7 cut(s) 125, 146, 189, 244, 315, 539, 642
SfcI CTRYAG 2 cut(s) 157, 593
Sfr274I CTCGAG 1 cut(s) 449
SfuI TTCGAA 1 cut(s) 368
SinI GGWCC 2 cut(s) 151, 338
SlaI CTCGAG 1 cut(s) 449
SmiMI CAYNNNNRTG 3 cut(s) 206, 212, 439
SmlI CTYRAG 3 cut(s) 329, 351, 449
SmoI CTYRAG 3 cut(s) 329, 351, 449
Sse9I AATT 1 cut(s) 370
SsiI CCGC 7 cut(s) 25, 37, 74, 95, 262, 430, 546
TaaI ACNGT 2 cut(s) 529, 579
TaiI ACGT 1 cut(s) 146
TaqI TCGA 3 cut(s) 309, 368, 450
TasI AATT 1 cut(s) 370
TatI WGTACW 2 cut(s) 210, 231
TauI GCSGC 2 cut(s) 97, 433
TfiI GAWTC 1 cut(s) 470
TscAI CASTG 2 cut(s) 602, 626
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 3 cut(s) 616, 637, 640
Tsp45I GTSAC 1 cut(s) 412
TspRI CASTG 2 cut(s) 602, 626
Tth111I GACNNNGTC 1 cut(s) 185
VneI GTGCAC 1 cut(s) 598
VpaK11BI GGWCC 2 cut(s) 151, 338
XapI RAATTY 1 cut(s) 370
XceI RCATGY 1 cut(s) 211
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XhoI CTCGAG 1 cut(s) 449
XmnI GAANNNNTTC 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.