Rh1DG030500

Prolyl oligopeptidase, N-terminal beta-propeller domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0014832
Physical Location & Seq
Forward (+)
4550836 .. 4555086
4251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG030500.1

Sequence Viewer

Length: 621 bp
ATGCTCTACCCTGTAAAACCCCTCCGCCCCCATCTCCGCCATCTACGGCTGGCCCACTTCAAGGCCCCAAAACCGCCGGAATGCCCTCCGAAGCCGCAAAACCCTCTCCAGAAGCCCGAAACCTTTACCCTCCATGACCACACGTGGCAGGACCCCTACAGATGGATGTTCAAACTCAACGACAAGGTCGCCATGCGACACATGGACATGTACATGGAGCAGGAAGACAAGTACACCAAAGCTGTCATGTACGGCTCCGACCGCCTCCAGAGCAGGCTCCAGTCCGAGATTGCCTCTCGCTTATCCTTCGACCTCTCCACTCCTCCCCTCAAGTTCGGACCATGGTACGGAACCGTTGTACTACTGGCCGGCGCGTGGAGGAAAGTAAGCGGTTTCCGGTGCTGTGTCGTCGACTCGCGAGTTTACACGACGAGTTCATTTCTCATAAGTCGCCTTCTGCTGGGTTTGATTTTACTTCCGGGAAGAGAATTGAGCAGAAGTAAGCCTCAAGCGAAGCGTGTTTCGAATTTGTCTTGGGCCAAAGACGGGCAGGCATTGCTTTATGTTGTCACCAATCAGAACAAGCGGCCATATAGTTCCAAACAAAAGGAATGGGCTTGA

Protein Analysis

206

Amino Acids

24.0

Weight (kDa)

10.01

Isoelectric Point (pI)

52.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9_N PF02897 40 - 116 6.1e-07 Prolyl oligopeptidase, N-terminal beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 411
AccII CGCG 2 cut(s) 374, 418
AciI CCGC 7 cut(s) 25, 37, 74, 95, 262, 390, 586
AcoI YGGCCR 2 cut(s) 366, 587
AcsI RAATTY 1 cut(s) 526
AcvI CACGTG 1 cut(s) 144
AfaI GTAC 5 cut(s) 212, 233, 251, 347, 360
AfiI CCNNNNNNNGG 6 cut(s) 61, 162, 347, 375, 460, 546
AflIII ACRYGT 2 cut(s) 141, 207
AgsI TTSAA 2 cut(s) 61, 172
AluBI AGCT 1 cut(s) 242
AluI AGCT 1 cut(s) 242
AoxI GGCC 5 cut(s) 51, 63, 366, 537, 587
ApoI RAATTY 1 cut(s) 526
AspLEI GCGC 1 cut(s) 374
AspS9I GGNCC 5 cut(s) 52, 64, 151, 338, 537
AsuC2I CCSGG 1 cut(s) 480
AsuHPI GGTGA 1 cut(s) 562
AsuII TTCGAA 1 cut(s) 524
AvaII GGWCC 2 cut(s) 151, 338
BbrPI CACGTG 1 cut(s) 144
BbsI GAAGAC 1 cut(s) 231
BccI CCATC 3 cut(s) 39, 48, 156
BceAI ACGGC 2 cut(s) 62, 268
BcnI CCSGG 1 cut(s) 480
BfmI CTRYAG 1 cut(s) 157
BisI GCNGC 2 cut(s) 95, 587
BlsI GCNGC 2 cut(s) 96, 588
Bme1390I CCNGG 1 cut(s) 480
Bme18I GGWCC 2 cut(s) 151, 338
BmgT120I GGNCC 5 cut(s) 52, 64, 151, 338, 537
BmiI GGNNCC 5 cut(s) 66, 153, 256, 278, 352
BmrFI CCNGG 1 cut(s) 480
BpiI GAAGAC 1 cut(s) 231
BplI GAGNNNNNCTC 2 cut(s) 278, 310
BpmI CTGGAG 3 cut(s) 92, 251, 263
Bpu14I TTCGAA 1 cut(s) 524
BpuEI CTTGAG 2 cut(s) 314, 492
BpuMI CCSGG 1 cut(s) 480
BsaAI YACGTR 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 341
BsaWI WCCGGW 1 cut(s) 396
Bsc4I CCNNNNNNNGG 6 cut(s) 61, 162, 347, 375, 460, 546
Bse118I RCCGGY 1 cut(s) 368
Bse1I ACTGG 2 cut(s) 280, 369
Bse3DI GCAATG 1 cut(s) 554
BseDI CCNNGG 1 cut(s) 341
BseGI GGATG 1 cut(s) 171
BseLI CCNNNNNNNGG 6 cut(s) 61, 162, 347, 375, 460, 546
BseMI GCAATG 1 cut(s) 554
BseNI ACTGG 2 cut(s) 280, 369
BseRI GAGGAG 1 cut(s) 312
BseYI CCCAGC 1 cut(s) 460
Bsh1236I CGCG 2 cut(s) 374, 418
Bsh1285I CGRYCG 1 cut(s) 262
BshFI GGCC 5 cut(s) 53, 65, 368, 539, 589
BsiEI CGRYCG 1 cut(s) 262
BsiSI CCGG 4 cut(s) 77, 369, 397, 479
BslI CCNNNNNNNGG 6 cut(s) 61, 162, 347, 375, 460, 546
BsmI GAATGC 1 cut(s) 86
BsnI GGCC 5 cut(s) 53, 65, 368, 539, 589
Bsp119I TTCGAA 1 cut(s) 524
Bsp1407I TGTACA 1 cut(s) 210
Bsp19I CCATGG 1 cut(s) 341
Bsp68I TCGCGA 1 cut(s) 418
BspACI CCGC 7 cut(s) 25, 37, 74, 95, 262, 390, 586
BspANI GGCC 5 cut(s) 53, 65, 368, 539, 589
BspFNI CGCG 2 cut(s) 374, 418
BspLI GGNNCC 5 cut(s) 66, 153, 256, 278, 352
BspT104I TTCGAA 1 cut(s) 524
BsrDI GCAATG 1 cut(s) 554
BsrFI RCCGGY 1 cut(s) 368
BsrGI TGTACA 1 cut(s) 210
BsrI ACTGG 2 cut(s) 280, 369
BssAI RCCGGY 1 cut(s) 368
BssECI CCNNGG 1 cut(s) 341
BssT1I CCWWGG 1 cut(s) 341
Bst4CI ACNGT 1 cut(s) 355
Bst6I CTCTTC 1 cut(s) 478
BstAPI GCANNNNNTGC 1 cut(s) 556
BstAUI TGTACA 1 cut(s) 210
BstBAI YACGTR 1 cut(s) 144
BstBI TTCGAA 1 cut(s) 524
BstC8I GCNNGC 4 cut(s) 51, 275, 370, 552
BstDSI CCRYGG 1 cut(s) 341
BstF5I GGATG 1 cut(s) 171
BstFNI CGCG 2 cut(s) 374, 418
BstHHI GCGC 1 cut(s) 374
BstMCI CGRYCG 1 cut(s) 262
BstMWI GCNNNNNNNGC 3 cut(s) 261, 270, 556
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 1 cut(s) 478
BstSFI CTRYAG 1 cut(s) 157
BstUI CGCG 2 cut(s) 374, 418
BstV2I GAAGAC 1 cut(s) 231
BsuRI GGCC 5 cut(s) 53, 65, 368, 539, 589
BtgI CCRYGG 1 cut(s) 341
BtsCI GGATG 1 cut(s) 171
BtuMI TCGCGA 1 cut(s) 418
Cac8I GCNNGC 4 cut(s) 51, 275, 370, 552
CfoI GCGC 1 cut(s) 374
Cfr10I RCCGGY 1 cut(s) 368
Cfr13I GGNCC 5 cut(s) 52, 64, 151, 338, 537
Csp6I GTAC 5 cut(s) 211, 232, 250, 346, 359
CviAII CATG 7 cut(s) 134, 193, 202, 208, 214, 247, 342
CviQI GTAC 5 cut(s) 211, 232, 250, 346, 359
EaeI YGGCCR 2 cut(s) 366, 587
Eam1104I CTCTTC 1 cut(s) 478
EarI CTCTTC 1 cut(s) 478
EciI GGCGGA 2 cut(s) 14, 26
Eco130I CCWWGG 1 cut(s) 341
Eco47I GGWCC 2 cut(s) 151, 338
Eco72I CACGTG 1 cut(s) 144
EcoO109I RGGNCCY 2 cut(s) 64, 151
EcoT14I CCWWGG 1 cut(s) 341
ErhI CCWWGG 1 cut(s) 341
FaeI CATG 7 cut(s) 137, 196, 205, 211, 217, 250, 345
FatI CATG 7 cut(s) 133, 192, 201, 207, 213, 246, 341
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 2 cut(s) 95, 587
FokI GGATG 1 cut(s) 178
Fsp4HI GCNGC 2 cut(s) 95, 587
GlaI GCGC 1 cut(s) 373
GluI GCNGC 2 cut(s) 95, 587
GsaI CCCAGC 1 cut(s) 464
GsuI CTGGAG 3 cut(s) 92, 251, 263
HaeIII GGCC 5 cut(s) 53, 65, 368, 539, 589
HapII CCGG 4 cut(s) 77, 369, 397, 479
HhaI GCGC 1 cut(s) 374
Hin1II CATG 7 cut(s) 137, 196, 205, 211, 217, 250, 345
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 1 cut(s) 413
HpaII CCGG 4 cut(s) 77, 369, 397, 479
HphI GGTGA 1 cut(s) 562
Hpy166II GTNNAC 3 cut(s) 234, 412, 424
Hpy188I TCNGA 5 cut(s) 90, 259, 286, 338, 579
Hpy188III TCNNGA 3 cut(s) 109, 268, 417
Hpy8I GTNNAC 3 cut(s) 234, 412, 424
Hpy99I CGWCG 2 cut(s) 413, 433
HpyAV CCTTC 2 cut(s) 316, 464
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 1 cut(s) 143
HpyF10VI GCNNNNNNNGC 3 cut(s) 261, 270, 556
HpySE526I ACGT 1 cut(s) 143
Hsp92II CATG 7 cut(s) 137, 196, 205, 211, 217, 250, 345
HspAI GCGC 1 cut(s) 372
KroI GCCGGC 1 cut(s) 368
KroNI GCCGGC 1 cut(s) 370
LmnI GCTCC 3 cut(s) 217, 260, 282
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 1 cut(s) 568
MboII GAAGA 2 cut(s) 236, 495
MluCI AATT 2 cut(s) 488, 526
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 1 cut(s) 282
MroNI GCCGGC 1 cut(s) 368
MslI CAYNNNNRTG 2 cut(s) 206, 212
MspI CCGG 4 cut(s) 77, 369, 397, 479
MspR9I CCNGG 1 cut(s) 480
Mva1269I GAATGC 1 cut(s) 86
MvnI CGCG 2 cut(s) 374, 418
MwoI GCNNNNNNNGC 3 cut(s) 261, 270, 556
NaeI GCCGGC 1 cut(s) 370
NciI CCSGG 1 cut(s) 480
NcoI CCATGG 1 cut(s) 341
NgoMIV GCCGGC 1 cut(s) 368
NlaIII CATG 7 cut(s) 137, 196, 205, 211, 217, 250, 345
NlaIV GGNNCC 5 cut(s) 66, 153, 256, 278, 352
NmuCI GTSAC 1 cut(s) 568
NruI TCGCGA 1 cut(s) 418
NspI RCATGY 1 cut(s) 211
NspV TTCGAA 1 cut(s) 524
PciI ACATGT 1 cut(s) 207
PctI GAATGC 1 cut(s) 86
PdiI GCCGGC 1 cut(s) 370
PflFI GACNNNGTC 1 cut(s) 185
PfoI TCCNGGA 1 cut(s) 478
PkrI GCNGC 2 cut(s) 96, 588
PleI GAGTC 1 cut(s) 407
PmaCI CACGTG 1 cut(s) 144
PmlI CACGTG 1 cut(s) 144
PpsI GAGTC 1 cut(s) 407
Ppu21I YACGTR 1 cut(s) 144
PpuMI RGGWCCY 1 cut(s) 151
PscI ACATGT 1 cut(s) 207
Psp5II RGGWCCY 1 cut(s) 151
PspCI CACGTG 1 cut(s) 144
PspFI CCCAGC 1 cut(s) 460
PspN4I GGNNCC 5 cut(s) 66, 153, 256, 278, 352
PspPI GGNCC 5 cut(s) 52, 64, 151, 338, 537
PspPPI RGGWCCY 1 cut(s) 151
PsyI GACNNNGTC 1 cut(s) 185
RruI TCGCGA 1 cut(s) 418
RsaI GTAC 5 cut(s) 212, 233, 251, 347, 360
RsaNI GTAC 5 cut(s) 211, 232, 250, 346, 359
RseI CAYNNNNRTG 2 cut(s) 206, 212
SalI GTCGAC 1 cut(s) 410
SatI GCNGC 2 cut(s) 95, 587
Sau96I GGNCC 5 cut(s) 52, 64, 151, 338, 537
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 1 cut(s) 480
SetI ASST 5 cut(s) 125, 146, 189, 244, 315
SfcI CTRYAG 1 cut(s) 157
SfuI TTCGAA 1 cut(s) 524
SinI GGWCC 2 cut(s) 151, 338
SmiMI CAYNNNNRTG 2 cut(s) 206, 212
SmlI CTYRAG 2 cut(s) 329, 507
SmoI CTYRAG 2 cut(s) 329, 507
Sse9I AATT 2 cut(s) 488, 526
SsiI CCGC 7 cut(s) 25, 37, 74, 95, 262, 390, 586
StyD4I CCNGG 1 cut(s) 478
StyI CCWWGG 1 cut(s) 341
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 1 cut(s) 146
TaqI TCGA 3 cut(s) 309, 411, 524
TasI AATT 2 cut(s) 488, 526
TatI WGTACW 3 cut(s) 210, 231, 358
TauI GCSGC 2 cut(s) 97, 589
TseFI GTSAC 1 cut(s) 568
Tsp45I GTSAC 1 cut(s) 568
TspDTI ATGAA 1 cut(s) 426
TspGWI ACGGA 1 cut(s) 363
Tth111I GACNNNGTC 1 cut(s) 185
VpaK11BI GGWCC 2 cut(s) 151, 338
XapI RAATTY 1 cut(s) 526
XceI RCATGY 1 cut(s) 211
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.