pycom02g19460

Nucleolar GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005154
Physical Location & Seq
Reverse (-)
17314297 .. 17321178
6882 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1335 bp
ATGAGCAGGGCTTGCAGTCTCTTCCAATTGTGGCAGACCCCTAACAGTTTACCACTCACACTCTCCAAACTTTCAAAAGGTCCGCACTTGATACCGAGTGCCAGAAATTATCCGGTTCTGTGCTTCTGCAAGGAAATACAAACTAGCACATACGAAGTTGTCAAGGGAAGCTATGTACCGTCAGCCGTTGTAAAACCGGAAAGCAGGCCGAAGGAAAAATCACCCGAAAATCTTGAAGATGTTGGTGCATTCCAAAAGCTACCCATGGTGATGCCATCTGTTGACATTCTGTATTCGGCACTAAGGAAGGCTAAGAGGATTACACCAACAAAGGGTATTGCCAATATTGCAAAGCGAGAAAGAAATAGAGGTGCAAAGCAACTTGATGCATTGATGAAAGAACTGGCAATTCCATTGAGAACTTATTCGGAGAATTTTCCAAATAAAAAGTGTCTTCATCCTTATGAACGGTCTCTTATTGAATTAACTCTTGGGGATGGAAATTATGAACAGGTGTTGGGAAAGGTGGATGCTTTGAGGAAGAAGGTTGTGTCTATTGGAAAGGAACATGCATCTCTTTGTGCTAAGTCAACGACGAAGAAGGAAGCAGAGGCAAGACTAACTGAGGGTATAAAGAAAGTTGAAGAGACATTTAATCGTGAAGGAAAACATGTTGATGATTTGTTACACATAGCCAAGACTCTGCGAGCAATGCCAGTAGTTGATCTAGAAGCACCAACACTTTGTCTTGTTGGAGCTCCTAACGTTGGGAAGTCATCTTTGGTTCGTATACTCTCCACTGGGAAGCCTGAGGTCTGCAACTACCCGTTCACAACAAGGGGAATACTGATGGGTCATATTATATCAAACCGTCAAACTTTTCAGGTGACAGACACTCCTGGCCTGCTGAAGAGATGTGATGAGGACAGAAATAATTTGGAAAAATTGACACTTGCTGTCCTCTCAAATTTACCAACCGCAATTCTCTATGTCCATGACCTCTCTGGGGAATGCGGGACCTCACCTTCTGACCAGTTTGTCATATACAAAGAAATAAAGGAGAGGTTTGGTGAGCATTTGTGGCTTGATGTTGTGTCCAAATGTGATCTGTTGCAAAAGTCTCCTGTGCTATTTGCCACTGATAATGGTTATGATTCTGATCTTAAGCTGGAAAGGTACCGGAAATTGGGTCCTGATGGAGCTATTCATGTGTCAGTGACGAATGAAGTAGGGCTCACCGAGTTGAAGATTAGAGTGCATGAGATGCTAGATTCACAGATGACGAGGATCCAAGTTCAAGAAAGCAACCAAGAAAAGCTTGAAGTTGGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
MMR_HSR1 NOG1

Protein Analysis

445

Amino Acids

49.59

Weight (kDa)

8.89

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOG1_N PF17835 84 - 242 8.3e-50 NOG1 N-terminal helical domain
FeoB_N PF02421 247 - 304 7e-09 Ferrous iron transport protein B
MMR_HSR1 PF01926 248 - 344 4.8e-16 50S ribosome-binding GTPase
NOG1 PF06858 313 - 369 7.4e-17 Nucleolar GTP-binding protein 1 (NOG1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1037
Acc65I GGTACC 1 cut(s) 1176
AccB1I GGYRCC 1 cut(s) 1176
AccI GTMKAC 1 cut(s) 790
AciI CCGC 3 cut(s) 83, 978, 1014
AclI AACGTT 1 cut(s) 765
AclWI GGATC 2 cut(s) 1282, 1295
AcsI RAATTY 2 cut(s) 433, 967
AcuI CTGAAG 1 cut(s) 929
AfaI GTAC 3 cut(s) 177, 1178, 1330
AfiI CCNNNNNNNGG 4 cut(s) 332, 767, 1006, 1186
AflII CTTAAG 1 cut(s) 1163
AflIII ACRYGT 1 cut(s) 670
AgsI TTSAA 7 cut(s) 75, 236, 482, 644, 1246, 1298, 1322
AjnI CCWGG 1 cut(s) 898
AjuI GAANNNNNNNTTGG 4 cut(s) 474, 506, 764, 796
AluBI AGCT 6 cut(s) 171, 259, 758, 1168, 1202, 1318
AluI AGCT 6 cut(s) 171, 259, 758, 1168, 1202, 1318
Alw21I GWGCWC 1 cut(s) 760
Alw26I GTCTC 4 cut(s) 23, 477, 641, 1125
AlwI GGATC 2 cut(s) 1282, 1295
AoxI GGCC 2 cut(s) 206, 901
ApoI RAATTY 2 cut(s) 433, 967
Asp700I GAANNNNTTC 1 cut(s) 424
Asp718I GGTACC 1 cut(s) 1176
AspS9I GGNCC 3 cut(s) 80, 1017, 1190
AsuHPI GGTGA 6 cut(s) 213, 280, 898, 1014, 1082, 1228
AvaII GGWCC 3 cut(s) 80, 1017, 1190
AxyI CCTNAGG 1 cut(s) 810
BamHI GGATCC 1 cut(s) 1287
BanI GGYRCC 1 cut(s) 1176
BanII GRGCYC 2 cut(s) 760, 1236
BbsI GAAGAC 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 760
BccI CCATC 4 cut(s) 283, 491, 844, 1190
BceAI ACGGC 1 cut(s) 170
BciT130I CCWGG 1 cut(s) 900
BcoDI GTCTC 4 cut(s) 23, 477, 641, 1125
BfaI CTAG 3 cut(s) 144, 728, 1268
BfrI CTTAAG 1 cut(s) 1163
Bme1390I CCNGG 1 cut(s) 900
Bme18I GGWCC 3 cut(s) 80, 1017, 1190
BmgT120I GGNCC 3 cut(s) 80, 1017, 1190
BmiI GGNNCC 4 cut(s) 1018, 1178, 1191, 1289
BmrFI CCNGG 1 cut(s) 900
BmrI ACTGGG 1 cut(s) 810
BmsI GCATC 5 cut(s) 261, 376, 520, 581, 1254
BmuI ACTGGG 1 cut(s) 810
BpiI GAAGAC 1 cut(s) 446
BsaBI GATNNNNATC 1 cut(s) 1158
BsaI GGTCTC 1 cut(s) 477
BsaJI CCNNGG 1 cut(s) 264
BsaWI WCCGGW 3 cut(s) 112, 196, 1179
BsaXI ACNNNNNCTCC 2 cut(s) 880, 910
Bsc4I CCNNNNNNNGG 4 cut(s) 332, 767, 1006, 1186
Bse1I ACTGG 4 cut(s) 408, 716, 805, 1033
Bse21I CCTNAGG 1 cut(s) 810
Bse3DI GCAATG 1 cut(s) 717
Bse8I GATNNNNATC 1 cut(s) 1158
BseBI CCWGG 1 cut(s) 900
BseDI CCNNGG 1 cut(s) 264
BseGI GGATG 3 cut(s) 457, 502, 535
BseJI GATNNNNATC 1 cut(s) 1158
BseLI CCNNNNNNNGG 4 cut(s) 332, 767, 1006, 1186
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 2 cut(s) 615, 801
BseNI ACTGG 4 cut(s) 408, 716, 805, 1033
BshFI GGCC 2 cut(s) 208, 903
BshNI GGYRCC 1 cut(s) 1176
BsiHKAI GWGCWC 1 cut(s) 760
BsiSI CCGG 3 cut(s) 113, 197, 1180
BslFI GGGAC 1 cut(s) 1030
BslI CCNNNNNNNGG 4 cut(s) 332, 767, 1006, 1186
BsmAI GTCTC 4 cut(s) 23, 477, 641, 1125
BsmFI GGGAC 1 cut(s) 1030
BsmI GAATGC 2 cut(s) 248, 1016
BsnI GGCC 2 cut(s) 208, 903
Bso31I GGTCTC 1 cut(s) 477
Bsp1286I GDGCHC 2 cut(s) 760, 1236
Bsp143I GATC 4 cut(s) 724, 1105, 1159, 1287
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 3 cut(s) 83, 978, 1014
BspANI GGCC 2 cut(s) 208, 903
BspCNI CTCAG 2 cut(s) 616, 802
BspLI GGNNCC 4 cut(s) 1018, 1178, 1191, 1289
BspPI GGATC 2 cut(s) 1282, 1295
BspT107I GGYRCC 1 cut(s) 1176
BspTI CTTAAG 1 cut(s) 1163
BspTNI GGTCTC 1 cut(s) 477
BsrDI GCAATG 1 cut(s) 717
BsrI ACTGG 4 cut(s) 408, 716, 805, 1033
BssECI CCNNGG 1 cut(s) 264
BssMI GATC 4 cut(s) 724, 1105, 1159, 1287
BssNAI GTATAC 1 cut(s) 791
BssT1I CCWWGG 1 cut(s) 264
Bst1107I GTATAC 1 cut(s) 791
Bst2UI CCWGG 1 cut(s) 900
Bst4CI ACNGT 4 cut(s) 47, 180, 471, 872
Bst6I CTCTTC 3 cut(s) 26, 639, 905
BstAFI CTTAAG 1 cut(s) 1163
BstAPI GCANNNNNTGC 2 cut(s) 12, 1264
BstC8I GCNNGC 4 cut(s) 13, 206, 708, 905
BstDEI CTNAG 5 cut(s) 302, 312, 585, 624, 810
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 3 cut(s) 457, 502, 535
BstKTI GATC 4 cut(s) 727, 1108, 1162, 1290
BstMAI GTCTC 4 cut(s) 23, 477, 641, 1125
BstMBI GATC 4 cut(s) 724, 1105, 1159, 1287
BstMWI GCNNNNNNNGC 5 cut(s) 12, 347, 712, 1081, 1264
BstNI CCWGG 1 cut(s) 900
BstNSI RCATGY 2 cut(s) 572, 674
BstSCI CCNGG 1 cut(s) 898
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 1 cut(s) 1287
BstYI RGATCY 1 cut(s) 1287
BstZ17I GTATAC 1 cut(s) 791
Bsu36I CCTNAGG 1 cut(s) 810
BsuRI GGCC 2 cut(s) 208, 903
BtgI CCRYGG 1 cut(s) 264
BtsCI GGATG 3 cut(s) 457, 502, 535
BtsIMutI CAGTG 3 cut(s) 798, 1137, 1221
Cac8I GCNNGC 4 cut(s) 13, 206, 708, 905
Cfr13I GGNCC 3 cut(s) 80, 1017, 1190
Csp6I GTAC 3 cut(s) 176, 1177, 1329
CviAII CATG 6 cut(s) 265, 569, 671, 995, 1208, 1259
CviQI GTAC 3 cut(s) 176, 1177, 1329
DdeI CTNAG 5 cut(s) 302, 312, 585, 624, 810
DpnI GATC 4 cut(s) 726, 1107, 1161, 1289
DpnII GATC 4 cut(s) 724, 1105, 1159, 1287
DrdI GACNNNNNNGTC 1 cut(s) 1037
DseDI GACNNNNNNGTC 1 cut(s) 1037
Eam1104I CTCTTC 3 cut(s) 26, 639, 905
EarI CTCTTC 3 cut(s) 26, 639, 905
Ecl136II GAGCTC 1 cut(s) 758
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 2 cut(s) 760, 1236
Eco31I GGTCTC 1 cut(s) 477
Eco47I GGWCC 3 cut(s) 80, 1017, 1190
Eco53kI GAGCTC 1 cut(s) 758
Eco57I CTGAAG 1 cut(s) 929
Eco81I CCTNAGG 1 cut(s) 810
EcoICRI GAGCTC 1 cut(s) 758
EcoO109I RGGNCCY 2 cut(s) 1017, 1190
EcoRII CCWGG 1 cut(s) 898
EcoT14I CCWWGG 1 cut(s) 264
EcoT22I ATGCAT 2 cut(s) 391, 574
EcoT38I GRGCYC 2 cut(s) 760, 1236
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 6 cut(s) 268, 572, 674, 998, 1211, 1262
FalI AAGNNNNNCTT 2 cut(s) 1302, 1334
FaqI GGGAC 1 cut(s) 1030
FatI CATG 6 cut(s) 264, 568, 670, 994, 1207, 1258
FauI CCCGC 1 cut(s) 1007
FblI GTMKAC 1 cut(s) 790
FokI GGATG 3 cut(s) 444, 509, 542
FriOI GRGCYC 2 cut(s) 760, 1236
FspBI CTAG 3 cut(s) 144, 728, 1268
HaeIII GGCC 2 cut(s) 208, 903
HapII CCGG 3 cut(s) 113, 197, 1180
Hin1II CATG 6 cut(s) 268, 572, 674, 998, 1211, 1262
HincII GTYRAC 2 cut(s) 283, 591
HindII GTYRAC 2 cut(s) 283, 591
HindIII AAGCTT 1 cut(s) 1316
HinfI GANTC 3 cut(s) 700, 1154, 1271
HpaII CCGG 3 cut(s) 113, 197, 1180
HphI GGTGA 6 cut(s) 213, 280, 898, 1014, 1082, 1228
Hpy166II GTNNAC 5 cut(s) 50, 283, 591, 791, 831
Hpy188I TCNGA 3 cut(s) 430, 1030, 1159
Hpy188III TCNNGA 5 cut(s) 233, 659, 728, 1193, 1298
Hpy8I GTNNAC 5 cut(s) 50, 283, 591, 791, 831
Hpy99I CGWCG 1 cut(s) 598
HpyAV CCTTC 6 cut(s) 205, 301, 538, 595, 656, 1035
HpyCH4III ACNGT 4 cut(s) 47, 180, 471, 872
HpyCH4IV ACGT 1 cut(s) 765
HpyF10VI GCNNNNNNNGC 5 cut(s) 12, 347, 712, 1081, 1264
HpyF3I CTNAG 5 cut(s) 302, 312, 585, 624, 810
HpySE526I ACGT 1 cut(s) 765
Hsp92II CATG 6 cut(s) 268, 572, 674, 998, 1211, 1262
KpnI GGTACC 1 cut(s) 1180
Kzo9I GATC 4 cut(s) 724, 1105, 1159, 1287
LmnI GCTCC 3 cut(s) 755, 763, 1199
LweI GCATC 5 cut(s) 261, 376, 520, 581, 1254
MaeI CTAG 3 cut(s) 144, 728, 1268
MaeII ACGT 1 cut(s) 765
MaeIII GTNAC 3 cut(s) 684, 886, 1216
MalI GATC 4 cut(s) 726, 1107, 1161, 1289
MboI GATC 4 cut(s) 724, 1105, 1159, 1287
MboII GAAGA 8 cut(s) 13, 248, 446, 553, 610, 656, 922, 1258
MfeI CAATTG 1 cut(s) 26
MflI RGATCY 1 cut(s) 1287
MhlI GDGCHC 2 cut(s) 760, 1236
MlyI GAGTC 1 cut(s) 694
MmeI TCCRAC 1 cut(s) 733
Mph1103I ATGCAT 2 cut(s) 391, 574
MroXI GAANNNNTTC 1 cut(s) 424
MseI TTAA 3 cut(s) 485, 654, 1164
MslI CAYNNNNRTG 3 cut(s) 269, 462, 675
MspCI CTTAAG 1 cut(s) 1163
MspI CCGG 3 cut(s) 113, 197, 1180
MspR9I CCNGG 1 cut(s) 900
MunI CAATTG 1 cut(s) 26
Mva1269I GAATGC 2 cut(s) 248, 1016
MvaI CCWGG 1 cut(s) 900
MwoI GCNNNNNNNGC 5 cut(s) 12, 347, 712, 1081, 1264
NcoI CCATGG 1 cut(s) 264
NdeII GATC 4 cut(s) 724, 1105, 1159, 1287
NlaIII CATG 6 cut(s) 268, 572, 674, 998, 1211, 1262
NlaIV GGNNCC 4 cut(s) 1018, 1178, 1191, 1289
NmuCI GTSAC 2 cut(s) 886, 1216
NsiI ATGCAT 2 cut(s) 391, 574
NspI RCATGY 2 cut(s) 572, 674
PciI ACATGT 1 cut(s) 670
PctI GAATGC 2 cut(s) 248, 1016
PdmI GAANNNNTTC 1 cut(s) 424
PfeI GAWTC 2 cut(s) 1154, 1271
PleI GAGTC 1 cut(s) 694
PpsI GAGTC 1 cut(s) 694
PpuMI RGGWCCY 2 cut(s) 1017, 1190
PscI ACATGT 1 cut(s) 670
Psp124BI GAGCTC 1 cut(s) 760
Psp1406I AACGTT 1 cut(s) 765
Psp5II RGGWCCY 2 cut(s) 1017, 1190
Psp6I CCWGG 1 cut(s) 898
PspGI CCWGG 1 cut(s) 898
PspN4I GGNNCC 4 cut(s) 1018, 1178, 1191, 1289
PspPI GGNCC 3 cut(s) 80, 1017, 1190
PspPPI RGGWCCY 2 cut(s) 1017, 1190
PsuI RGATCY 1 cut(s) 1287
RsaI GTAC 3 cut(s) 177, 1178, 1330
RsaNI GTAC 3 cut(s) 176, 1177, 1329
RseI CAYNNNNRTG 3 cut(s) 269, 462, 675
SacI GAGCTC 1 cut(s) 760
SaqAI TTAA 3 cut(s) 485, 654, 1164
Sau3AI GATC 4 cut(s) 724, 1105, 1159, 1287
Sau96I GGNCC 3 cut(s) 80, 1017, 1190
SchI GAGTC 1 cut(s) 694
ScrFI CCNGG 1 cut(s) 900
SduI GDGCHC 2 cut(s) 760, 1236
SfaNI GCATC 5 cut(s) 261, 376, 520, 581, 1254
SinI GGWCC 3 cut(s) 80, 1017, 1190
SmiMI CAYNNNNRTG 3 cut(s) 269, 462, 675
SmlI CTYRAG 1 cut(s) 1163
SmoI CTYRAG 1 cut(s) 1163
SsiI CCGC 3 cut(s) 83, 978, 1014
SspI AATATT 1 cut(s) 346
SspMI CTAG 3 cut(s) 144, 728, 1268
SstI GAGCTC 1 cut(s) 760
StyD4I CCNGG 1 cut(s) 898
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 4 cut(s) 47, 180, 471, 872
TaiI ACGT 1 cut(s) 768
TfiI GAWTC 2 cut(s) 1154, 1271
Tru1I TTAA 3 cut(s) 485, 654, 1164
Tru9I TTAA 3 cut(s) 485, 654, 1164
TscAI CASTG 3 cut(s) 805, 1144, 1221
TseFI GTSAC 2 cut(s) 886, 1216
Tsp45I GTSAC 2 cut(s) 886, 1216
TspDTI ATGAA 6 cut(s) 410, 446, 480, 522, 1196, 1239
TspRI CASTG 3 cut(s) 805, 1144, 1221
Vha464I CTTAAG 1 cut(s) 1163
VpaK11BI GGWCC 3 cut(s) 80, 1017, 1190
XapI RAATTY 2 cut(s) 433, 967
XbaI TCTAGA 1 cut(s) 727
XceI RCATGY 2 cut(s) 572, 674
XcmI CCANNNNNNNNNTGG 1 cut(s) 1001
XmiI GTMKAC 1 cut(s) 790
XmnI GAANNNNTTC 1 cut(s) 424
XspI CTAG 3 cut(s) 144, 728, 1268
Zsp2I ATGCAT 2 cut(s) 391, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.