pycom02g22650

DNA gyrase subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0010284
Physical Location & Seq
Reverse (-)
20855962 .. 20871114
15153 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2841 bp
ATGAAACCCTCAGTCTCCCTCCACCCTCCTACCATGTCCCTAGCTTCCGGTCTCCGCTTGTCTTCCTCCATCCTCCGCTACCGCCTCGCCGCCCCGCTCAACCGGACCCGACTCTCCGGGCTCCGCCAGAACCTCTCGGAGCTCCGCTTCCTTTCAGCATCATCCTCCCGCACCGGGACCCGCGTCCGCCCAATTAAAGCCAGGCTCCTTGACGAACCCCAAAAGGAGGACCCCGGCGAGGGGCAAGATGGGAACGGGAGCGTGCTGGTTAAGGACACAAGCGAGAATAGCGAAAGAATTGTCCGCGTTGAGCTCCATAAGGAGGCCACTGAAGCCTACATGGCCTACGCTATGTCTGTCCTGCTCGGTCGCGCCCTGCCCGATGTTCGTGATGGTTTGAAGCCCGTGCACCGCCGGATTTTGTATGCAATGCACGAATTGGGGATTGTATCGCGGAAACCGTTCAAGAAATGTGCTAGGGTTGTTGGAGAGGTTCTAGGAAAATTCCATCCCCATGGGGACACTGCCGTGTATGATTCCTTGGTGCGAATGGCTCAGGATTTCTCCTTACGATTCCCACTAATCCAAGGGCATGGGAATTTTGGTTCTATTGATGCTGACCCTGCAGCTGCTATGCGTTACACGGAATGCAGATTGGAAGCACTGACAGAAGCAATGCTATTGGCAGATTTAGATCAAAATACGGTTGATTTTACTCCAAATTTTGATAATTCACAAAAAGAGCCATCAGTTTTACCTGCTCGACTTCCAACTTTATTACTGAATGGTGCTTCTGGGATTGCGGTTGGCATGGCAACCAACATTCCTCCGCATAATCTTGGGGAATTGGTAGATGTTCTTAGTGTATTAATTCACAATCCAGAAGCTACGTTGCAAGAATTACTAGAATACATGCCAGGACCCGATTTTCCAACTGGAGGACTGATTATGGGCAACCTTGGAATCTTGGACGCATATCGAACTGGAAAAGGACGTATTGTTGTAAGAGGGAAAACTGATGTTGAATTGCTTGATTCTAAAACAAAGAGAAATGCAATTATCATAAAAGAGATCCCTTACCAAACCAACAAAGCAGCTCTTGTGGAGAAGATAGCTGAACTTGTGGAAAATAAGATTTTGGAAGGCATAAGTGATATTAGGGACGAAAGTGATCGTTCTGGAATGCGTGTAGTCATTGAGCTTAAGCGAGGATCCGACCCATCAATTGTTTTAAATAATCTCTATCGCCTCACTTCTCTTCAATGCAGTTTTAGTTGTAACATGGTGGGTATTCATAATGGGCAACCTAAGCAGATGGGTCTAAAGGAATTGCTGCAGGCATTCTTCGACTTCAGATGCTCTGTTGTCGAGAGACGTGCTAAGTTCAAACTTTCACAAGCACAGGACAGAAGACATATTGTTGAGGGCATTGTTGTGGGCCTTGATAATTTGGATTCAGTAATTCATATACTAAGGGAAGCTTCGAGCAATGCAATTGCATCAGCTGGTTTAAGGACTGAGTTCAGTCTTTCTGAGAAACAAGCTGAAGCTATATTAGATATTAGTCTAAGAAGAATTACCCAGCTTGAGAGGAAAAAGTTTGTAAATGAAAGTGAATCGTTAAAGGAGCAAATTTCAAAATTAGAAGAACTATTGTCAAGCAAGAAATCCATGTTACAGTTGATTGAACAAGAAGCAAACGAGTTAAAAAGCAAGTTTTCCAGTCTGAGGCGTTCAGTGTTGGAGGACAGTGATGGTGGCCACGTTGATGACATAGATGTTATTCCTAATGAAGAAATGCTTTTGGCCTTCAGTGAAAAGGGCTATGTTAAGCGGATGAAACCTAACACTTTCAATCTTCAAAATAGAGGAACCATTGGTAAATCTGTTGGGAAATTGAGGGTAAACGATGCAATGTCAGACTTTATTGTTTGTCGTGCGCATGATCATGTTCTATACTTCAGTGATAAAGGAACAGTTTACTCTGGTCGTGCATATAAAATTCCTGAATGCACCCGCACAGCTGCTGGGACGCCATTGGTTCAGATCTTAGCACTCTCGGATGGCGAAAGGATTACTTCTGTTATTCCTGTGAGTGAATTTTCTGAAGATCAATTTCTACTTATGCTTACAGTAAATGGCTACATCAAGAAGGTCTCTTTGAGTTCTTTTTCGTCAATCAGGTCAACAGGAATCATAGCAATCCAATTGGTTCCTGGTGATGAACTGAAATGGGTTCGTTGCTGCACAAATGATGACCTTGTAGCAATGGCTTCTCAGAATGGAATGGTCATCTTAAGTTCCTCTGGTATTATTCGAGCACAAGGGAGAAACACTAGGGGTGCAGTGGCCATGAGACTTAGAGAGGGGGATAAGATGGCTAGCGTGGACATTATACCAGCAGCCATGTGGAAAGACTTGGAGAGGGTGTCTGAAGCTCCCCAAGACACTACTAGAAGTCTGAAGGGCCCGTGGTTGTTATTTGTGTCTGAGAGTGGATATGGAAAGCGTGTTCCCTTAAGCAGATTTCACTCCTCCCGGTTGAATAGGGTTGGTCTGATAGGGTACAAGTTTGCCTTAGAGGATCGCTTGGCAGCAGTTTTTGTGGTCGGGTTCTCTGTGGCAGAAGATGGAGAAAGCGATGAACAAGTGGTTTTAGTAAGCCAAAGTGGCACAGTCAACAGAATTAAGGTGCGGGATATTTCAATACAGTCCCGCTATGCAAGGGGAGTTATTTTGATGCGGCTTGATCATGCTGGTAAGATTCAGTCTGCTTCGTTAATCTCAGCAACGGACGAGGAGCCTGAGGAGGTAGAAGTTGAAGCTGCCGTACATGGCTAA

Protein Analysis

947

Amino Acids

104.43

Weight (kDa)

7.94

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_topoisoIV PF00521 124 - 565 8.3e-160 DNA gyrase/topoisomerase IV, subunit A
DNA_gyraseA_C PF03989 599 - 645 2.1e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 706 - 749 3.9e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 753 - 799 1.7e-06 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 883 - 928 3.5e-06 DNA gyrase C-terminal domain, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1941
Acc36I ACCTGC 1 cut(s) 766
AccBSI CCGCTC 1 cut(s) 97
AccII CGCG 4 cut(s) 183, 306, 372, 454
AclWI GGATC 4 cut(s) 1066, 1206, 1219, 2592
AcoI YGGCCR 2 cut(s) 1759, 2349
AcsI RAATTY 6 cut(s) 503, 598, 721, 1632, 2001, 2099
AcuI CTGAAG 8 cut(s) 351, 1336, 1566, 1795, 1945, 2127, 2454, 2483
AcyI GRCGYC 1 cut(s) 2033
AfaI GTAC 2 cut(s) 2567, 2832
AfiI CCNNNNNNNGG 8 cut(s) 174, 226, 238, 239, 240, 322, 938, 1728
AflII CTTAAG 3 cut(s) 1202, 2296, 2518
AjiI CACGTC 1 cut(s) 1376
AjnI CCWGG 3 cut(s) 200, 916, 2215
AleI CACNNNNGTG 1 cut(s) 527
AloI GAACNNNNNNTCC 4 cut(s) 589, 621, 2496, 2528
Alw21I GWGCWC 4 cut(s) 144, 315, 411, 2323
Alw26I GTCTC 5 cut(s) 19, 56, 1366, 2161, 2350
Alw44I GTGCAC 1 cut(s) 407
AlwI GGATC 4 cut(s) 1066, 1206, 1219, 2592
AlwNI CAGNNNCTG 1 cut(s) 2027
AoxI GGCC 7 cut(s) 324, 342, 1438, 1759, 1806, 2349, 2467
ApaI GGGCCC 1 cut(s) 2471
ApaLI GTGCAC 1 cut(s) 407
ApeKI GCWGC 9 cut(s) 626, 629, 1094, 1333, 2024, 2244, 2402, 2594, 2825
ApoI RAATTY 6 cut(s) 503, 598, 721, 1632, 2001, 2099
ArsI GACNNNNNNTTYG 2 cut(s) 285, 317
AseI ATTAAT 1 cut(s) 869
Asp700I GAANNNNTTC 1 cut(s) 461
AspLEI GCGC 2 cut(s) 374, 1942
AspS9I GGNCC 7 cut(s) 105, 177, 229, 920, 1438, 2467, 2468
AsuC2I CCSGG 4 cut(s) 118, 175, 234, 2539
AsuHPI GGTGA 1 cut(s) 2231
AsuNHI GCTAGC 1 cut(s) 2381
AvaII GGWCC 4 cut(s) 105, 177, 229, 920
AxyI CCTNAGG 1 cut(s) 2805
BaeGI GKGCMC 2 cut(s) 411, 2471
BalI TGGCCA 2 cut(s) 1761, 2351
BamHI GGATCC 1 cut(s) 1211
BanII GRGCYC 4 cut(s) 123, 144, 315, 2471
BarI GAAGNNNNNNTAC 1 cut(s) 2814
BbsI GAAGAC 2 cut(s) 54, 1417
Bbv12I GWGCWC 4 cut(s) 144, 315, 411, 2323
BbvI GCAGC 9 cut(s) 616, 638, 1106, 1320, 2011, 2231, 2414, 2606, 2812
BceAI ACGGC 2 cut(s) 512, 2813
BcgI CGANNNNNNTGC 2 cut(s) 1358, 1392
BciT130I CCWGG 3 cut(s) 202, 918, 2217
BclI TGATCA 2 cut(s) 1945, 2749
BcnI CCSGG 4 cut(s) 118, 175, 234, 2539
BcoDI GTCTC 5 cut(s) 19, 56, 1366, 2161, 2350
BfaI CTAG 7 cut(s) 41, 477, 497, 905, 2337, 2382, 2454
BfmI CTRYAG 2 cut(s) 624, 1334
BfrI CTTAAG 3 cut(s) 1202, 2296, 2518
BfuAI ACCTGC 1 cut(s) 766
BglI GCCNNNNNGGC 2 cut(s) 341, 2670
BglII AGATCT 1 cut(s) 2046
Bme1390I CCNGG 7 cut(s) 118, 175, 202, 234, 918, 2217, 2539
Bme18I GGWCC 4 cut(s) 105, 177, 229, 920
BmgBI CACGTC 1 cut(s) 1376
BmgT120I GGNCC 7 cut(s) 105, 177, 229, 920, 1438, 2467, 2468
BmrFI CCNGG 7 cut(s) 118, 175, 202, 234, 918, 2217, 2539
BmsI GCATC 6 cut(s) 167, 604, 1346, 1508, 1900, 2730
BmtI GCTAGC 1 cut(s) 2385
BoxI GACNNNNGTC 1 cut(s) 182
BpiI GAAGAC 2 cut(s) 54, 1417
BpmI CTGGAG 1 cut(s) 957
Bpu10I CCTNAGC 2 cut(s) 555, 1308
BpuEI CTTGAG 1 cut(s) 1607
BpuMI CCSGG 4 cut(s) 118, 175, 234, 2539
BsaBI GATNNNNATC 1 cut(s) 693
BsaHI GRCGYC 1 cut(s) 2033
BsaI GGTCTC 2 cut(s) 56, 2161
BsaJI CCNNGG 6 cut(s) 232, 514, 540, 586, 958, 2471
BsaWI WCCGGW 2 cut(s) 47, 102
BsaXI ACNNNNNCTCC 2 cut(s) 2414, 2444
Bsc4I CCNNNNNNNGG 8 cut(s) 174, 226, 238, 239, 240, 322, 938, 1728
Bse1I ACTGG 3 cut(s) 940, 988, 1722
Bse21I CCTNAGG 1 cut(s) 2805
Bse3DI GCAATG 5 cut(s) 435, 681, 1495, 1920, 2274
Bse8I GATNNNNATC 1 cut(s) 693
BseBI CCWGG 3 cut(s) 202, 918, 2217
BseDI CCNNGG 6 cut(s) 232, 514, 540, 586, 958, 2471
BseGI GGATG 5 cut(s) 69, 161, 508, 1842, 2068
BseJI GATNNNNATC 1 cut(s) 693
BseLI CCNNNNNNNGG 8 cut(s) 174, 226, 238, 239, 240, 322, 938, 1728
BseMI GCAATG 5 cut(s) 435, 681, 1495, 1920, 2274
BseMII CTCAG 9 cut(s) 24, 569, 1509, 1524, 1718, 2291, 2481, 2796, 2799
BseNI ACTGG 3 cut(s) 940, 988, 1722
BseRI GAGGAG 3 cut(s) 2524, 2813, 2822
BseSI GKGCMC 2 cut(s) 411, 2471
BseXI GCAGC 9 cut(s) 616, 638, 1106, 1320, 2011, 2231, 2414, 2606, 2812
BseYI CCCAGC 2 cut(s) 1581, 2027
BsgI GTGCAG 2 cut(s) 2230, 2364
Bsh1236I CGCG 4 cut(s) 183, 306, 372, 454
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 7 cut(s) 326, 344, 1440, 1761, 1808, 2351, 2469
BsiEI CGRYCG 1 cut(s) 370
BsiHKAI GWGCWC 4 cut(s) 144, 315, 411, 2323
BsiSI CCGG 7 cut(s) 48, 103, 117, 174, 234, 415, 2539
BslFI GGGAC 6 cut(s) 22, 190, 533, 1175, 2044, 2698
BslI CCNNNNNNNGG 8 cut(s) 174, 226, 238, 239, 240, 322, 938, 1728
BsmAI GTCTC 5 cut(s) 19, 56, 1366, 2161, 2350
BsmBI CGTCTC 1 cut(s) 1366
BsmFI GGGAC 6 cut(s) 22, 190, 533, 1175, 2044, 2698
BsmI GAATGC 4 cut(s) 653, 1188, 1340, 2015
BsnI GGCC 7 cut(s) 326, 344, 1440, 1761, 1808, 2351, 2469
Bso31I GGTCTC 2 cut(s) 56, 2161
Bsp120I GGGCCC 1 cut(s) 2467
Bsp1286I GDGCHC 6 cut(s) 123, 144, 315, 411, 2323, 2471
Bsp143I GATC 9 cut(s) 694, 1071, 1171, 1211, 1945, 2046, 2110, 2584, 2749
Bsp19I CCATGG 1 cut(s) 514
BspANI GGCC 7 cut(s) 326, 344, 1440, 1761, 1808, 2351, 2469
BspCNI CTCAG 9 cut(s) 23, 568, 1510, 1525, 1719, 2290, 2482, 2797, 2798
BspFNI CGCG 4 cut(s) 183, 306, 372, 454
BspMAI CTGCAG 2 cut(s) 628, 1338
BspMI ACCTGC 1 cut(s) 766
BspOI GCTAGC 1 cut(s) 2385
BspPI GGATC 4 cut(s) 1066, 1206, 1219, 2592
BspTI CTTAAG 3 cut(s) 1202, 2296, 2518
BspTNI GGTCTC 2 cut(s) 56, 2161
BsrBI CCGCTC 1 cut(s) 97
BsrDI GCAATG 5 cut(s) 435, 681, 1495, 1920, 2274
BsrI ACTGG 3 cut(s) 940, 988, 1722
BssECI CCNNGG 6 cut(s) 232, 514, 540, 586, 958, 2471
BssMI GATC 9 cut(s) 694, 1071, 1171, 1211, 1945, 2046, 2110, 2584, 2749
BssNI GRCGYC 1 cut(s) 2033
BssT1I CCWWGG 4 cut(s) 514, 540, 586, 958
Bst2UI CCWGG 3 cut(s) 202, 918, 2217
Bst4CI ACNGT 8 cut(s) 462, 706, 1680, 1751, 1978, 2134, 2677, 2712
Bst6I CTCTTC 1 cut(s) 1263
BstACI GRCGYC 1 cut(s) 2033
BstAFI CTTAAG 3 cut(s) 1202, 2296, 2518
BstC8I GCNNGC 3 cut(s) 263, 1338, 2383
BstDSI CCRYGG 2 cut(s) 514, 2471
BstF5I GGATG 5 cut(s) 69, 161, 508, 1842, 2068
BstFNI CGCG 4 cut(s) 183, 306, 372, 454
BstHHI GCGC 2 cut(s) 374, 1942
BstKTI GATC 9 cut(s) 697, 1074, 1174, 1214, 1948, 2049, 2113, 2587, 2752
BstMAI GTCTC 5 cut(s) 19, 56, 1366, 2161, 2350
BstMBI GATC 9 cut(s) 694, 1071, 1171, 1211, 1945, 2046, 2110, 2584, 2749
BstMCI CGRYCG 1 cut(s) 370
BstMWI GCNNNNNNNGC 6 cut(s) 288, 332, 341, 623, 1309, 2670
BstNI CCWGG 3 cut(s) 202, 918, 2217
BstNSI RCATGY 1 cut(s) 916
BstPAI GACNNNNGTC 1 cut(s) 182
BstSCI CCNGG 7 cut(s) 116, 173, 200, 232, 916, 2215, 2537
BstSFI CTRYAG 2 cut(s) 624, 1334
BstSLI GKGCMC 2 cut(s) 411, 2471
BstUI CGCG 4 cut(s) 183, 306, 372, 454
BstV1I GCAGC 9 cut(s) 616, 638, 1106, 1320, 2011, 2231, 2414, 2606, 2812
BstV2I GAAGAC 2 cut(s) 54, 1417
BstX2I RGATCY 3 cut(s) 1071, 1211, 2046
BstXI CCANNNNNNTGG 2 cut(s) 515, 593
BstYI RGATCY 3 cut(s) 1071, 1211, 2046
Bsu36I CCTNAGG 1 cut(s) 2805
BsuRI GGCC 7 cut(s) 326, 344, 1440, 1761, 1808, 2351, 2469
BtgI CCRYGG 2 cut(s) 514, 2471
BtgZI GCGATG 1 cut(s) 2655
BtrI CACGTC 1 cut(s) 1376
BtsCI GGATG 5 cut(s) 69, 161, 508, 1842, 2068
BtsI GCAGTG 2 cut(s) 522, 2352
BtsIMutI CAGTG 8 cut(s) 327, 522, 662, 1743, 1756, 1819, 1969, 2352
BveI ACCTGC 1 cut(s) 766
Cac8I GCNNGC 3 cut(s) 263, 1338, 2383
CaiI CAGNNNCTG 1 cut(s) 2027
CfoI GCGC 2 cut(s) 374, 1942
Cfr13I GGNCC 7 cut(s) 105, 177, 229, 920, 1438, 2467, 2468
CseI GACGC 3 cut(s) 172, 980, 2041
Csp6I GTAC 2 cut(s) 2566, 2831
CviQI GTAC 2 cut(s) 2566, 2831
DpnI GATC 9 cut(s) 696, 1073, 1173, 1213, 1947, 2048, 2112, 2586, 2751
DpnII GATC 9 cut(s) 694, 1071, 1171, 1211, 1945, 2046, 2110, 2584, 2749
DraI TTTAAA 1 cut(s) 1233
EaeI YGGCCR 2 cut(s) 1759, 2349
Eam1104I CTCTTC 1 cut(s) 1263
EarI CTCTTC 1 cut(s) 1263
EciI GGCGGA 2 cut(s) 113, 176
Ecl136II GAGCTC 2 cut(s) 142, 313
Eco130I CCWWGG 4 cut(s) 514, 540, 586, 958
Eco24I GRGCYC 4 cut(s) 123, 144, 315, 2471
Eco31I GGTCTC 2 cut(s) 56, 2161
Eco47I GGWCC 4 cut(s) 105, 177, 229, 920
Eco53kI GAGCTC 2 cut(s) 142, 313
Eco57I CTGAAG 8 cut(s) 351, 1336, 1566, 1795, 1945, 2127, 2454, 2483
Eco81I CCTNAGG 1 cut(s) 2805
EcoICRI GAGCTC 2 cut(s) 142, 313
EcoO109I RGGNCCY 4 cut(s) 177, 229, 920, 2467
EcoRII CCWGG 3 cut(s) 200, 916, 2215
EcoT14I CCWWGG 4 cut(s) 514, 540, 586, 958
EcoT38I GRGCYC 4 cut(s) 123, 144, 315, 2471
ErhI CCWWGG 4 cut(s) 514, 540, 586, 958
Esp3I CGTCTC 1 cut(s) 1366
FaqI GGGAC 6 cut(s) 22, 190, 533, 1175, 2044, 2698
FauI CCCGC 6 cut(s) 102, 176, 188, 2024, 2688, 2723
FbaI TGATCA 2 cut(s) 1945, 2749
FokI GGATG 5 cut(s) 56, 148, 495, 1849, 2075
FriOI GRGCYC 4 cut(s) 123, 144, 315, 2471
FspAI RTGCGCAY 1 cut(s) 1941
FspBI CTAG 7 cut(s) 41, 477, 497, 905, 2337, 2382, 2454
FspI TGCGCA 1 cut(s) 1941
GlaI GCGC 2 cut(s) 373, 1941
GsaI CCCAGC 2 cut(s) 1585, 2031
GsuI CTGGAG 1 cut(s) 957
HaeIII GGCC 7 cut(s) 326, 344, 1440, 1761, 1808, 2351, 2469
HapII CCGG 7 cut(s) 48, 103, 117, 174, 234, 415, 2539
HgaI GACGC 3 cut(s) 172, 980, 2041
HhaI GCGC 2 cut(s) 374, 1942
Hin1I GRCGYC 1 cut(s) 2033
Hin6I GCGC 2 cut(s) 372, 1940
HinP1I GCGC 2 cut(s) 372, 1940
HincII GTYRAC 2 cut(s) 2187, 2680
HindII GTYRAC 2 cut(s) 2187, 2680
HindIII AAGCTT 1 cut(s) 1479
HinfI GANTC 9 cut(s) 111, 536, 573, 963, 1034, 1454, 1616, 2193, 2764
HpaII CCGG 7 cut(s) 48, 103, 117, 174, 234, 415, 2539
HphI GGTGA 1 cut(s) 2231
Hpy166II GTNNAC 6 cut(s) 409, 1906, 1981, 2187, 2389, 2680
Hpy188III TCNNGA 8 cut(s) 389, 466, 557, 881, 1179, 1369, 2006, 2149
Hpy8I GTNNAC 6 cut(s) 409, 1906, 1981, 2187, 2389, 2680
HpyAV CCTTC 4 cut(s) 1136, 1819, 2146, 2458
HpyCH4III ACNGT 8 cut(s) 462, 706, 1680, 1751, 1978, 2134, 2677, 2712
HpyCH4IV ACGT 4 cut(s) 890, 994, 1375, 1764
HpyF10VI GCNNNNNNNGC 6 cut(s) 288, 332, 341, 623, 1309, 2670
HpySE526I ACGT 4 cut(s) 890, 994, 1375, 1764
Hsp92I GRCGYC 1 cut(s) 2033
HspAI GCGC 2 cut(s) 372, 1940
KflI GGGWCCC 1 cut(s) 177
Ksp22I TGATCA 2 cut(s) 1945, 2749
Kzo9I GATC 9 cut(s) 694, 1071, 1171, 1211, 1945, 2046, 2110, 2584, 2749
LmnI GCTCC 9 cut(s) 126, 139, 147, 210, 258, 318, 1627, 2443, 2800
Lsp1109I GCAGC 9 cut(s) 616, 638, 1106, 1320, 2011, 2231, 2414, 2606, 2812
LweI GCATC 6 cut(s) 167, 604, 1346, 1508, 1900, 2730
MaeI CTAG 7 cut(s) 41, 477, 497, 905, 2337, 2382, 2454
MaeII ACGT 4 cut(s) 890, 994, 1375, 1764
MaeIII GTNAC 3 cut(s) 638, 1277, 1674
MalI GATC 9 cut(s) 696, 1073, 1173, 1213, 1947, 2048, 2112, 2586, 2751
MbiI CCGCTC 1 cut(s) 97
MboI GATC 9 cut(s) 694, 1071, 1171, 1211, 1945, 2046, 2110, 2584, 2749
MfeI CAATTG 3 cut(s) 1224, 1494, 2207
MflI RGATCY 3 cut(s) 1071, 1211, 2046
MhlI GDGCHC 6 cut(s) 123, 144, 315, 411, 2323, 2471
MlsI TGGCCA 2 cut(s) 1761, 2351
MluNI TGGCCA 2 cut(s) 1761, 2351
MlyI GAGTC 1 cut(s) 105
MmeI TCCRAC 5 cut(s) 466, 794, 956, 1239, 1722
Mox20I TGGCCA 2 cut(s) 1761, 2351
MroXI GAANNNNTTC 1 cut(s) 461
MscI TGGCCA 2 cut(s) 1761, 2351
MslI CAYNNNNRTG 5 cut(s) 513, 527, 1433, 1767, 1947
Msp20I TGGCCA 2 cut(s) 1761, 2351
MspA1I CMGCKG 3 cut(s) 629, 1505, 2024
MspCI CTTAAG 3 cut(s) 1202, 2296, 2518
MspI CCGG 7 cut(s) 48, 103, 117, 174, 234, 415, 2539
MspR9I CCNGG 7 cut(s) 118, 175, 202, 234, 918, 2217, 2539
MunI CAATTG 3 cut(s) 1224, 1494, 2207
Mva1269I GAATGC 4 cut(s) 653, 1188, 1340, 2015
MvaI CCWGG 3 cut(s) 202, 918, 2217
MvnI CGCG 4 cut(s) 183, 306, 372, 454
MwoI GCNNNNNNNGC 6 cut(s) 288, 332, 341, 623, 1309, 2670
NciI CCSGG 4 cut(s) 118, 175, 234, 2539
NcoI CCATGG 1 cut(s) 514
NdeII GATC 9 cut(s) 694, 1071, 1171, 1211, 1945, 2046, 2110, 2584, 2749
NheI GCTAGC 1 cut(s) 2381
NsbI TGCGCA 1 cut(s) 1941
NspI RCATGY 1 cut(s) 916
OliI CACNNNNGTG 1 cut(s) 527
PcsI WCGNNNNNNNCGW 1 cut(s) 458
PctI GAATGC 4 cut(s) 653, 1188, 1340, 2015
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 8 cut(s) 536, 573, 963, 1034, 1454, 1616, 2193, 2764
PleI GAGTC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 105
PpuMI RGGWCCY 3 cut(s) 177, 229, 920
PshAI GACNNNNGTC 1 cut(s) 182
PshBI ATTAAT 1 cut(s) 869
Psp124BI GAGCTC 2 cut(s) 144, 315
Psp5II RGGWCCY 3 cut(s) 177, 229, 920
Psp6I CCWGG 3 cut(s) 200, 916, 2215
PspFI CCCAGC 2 cut(s) 1581, 2027
PspGI CCWGG 3 cut(s) 200, 916, 2215
PspOMI GGGCCC 1 cut(s) 2467
PspPI GGNCC 7 cut(s) 105, 177, 229, 920, 1438, 2467, 2468
PspPPI RGGWCCY 3 cut(s) 177, 229, 920
PstI CTGCAG 2 cut(s) 628, 1338
PstNI CAGNNNCTG 1 cut(s) 2027
PsuI RGATCY 3 cut(s) 1071, 1211, 2046
PvuII CAGCTG 3 cut(s) 629, 1505, 2024
RsaI GTAC 2 cut(s) 2567, 2832
RsaNI GTAC 2 cut(s) 2566, 2831
RseI CAYNNNNRTG 5 cut(s) 513, 527, 1433, 1767, 1947
SacI GAGCTC 2 cut(s) 144, 315
Sau3AI GATC 9 cut(s) 694, 1071, 1171, 1211, 1945, 2046, 2110, 2584, 2749
Sau96I GGNCC 7 cut(s) 105, 177, 229, 920, 1438, 2467, 2468
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 7 cut(s) 118, 175, 202, 234, 918, 2217, 2539
SduI GDGCHC 6 cut(s) 123, 144, 315, 411, 2323, 2471
SfaNI GCATC 6 cut(s) 167, 604, 1346, 1508, 1900, 2730
SfcI CTRYAG 2 cut(s) 624, 1334
SinI GGWCC 4 cut(s) 105, 177, 229, 920
SmiMI CAYNNNNRTG 5 cut(s) 513, 527, 1433, 1767, 1947
SmlI CTYRAG 4 cut(s) 1202, 1586, 2296, 2518
SmoI CTYRAG 4 cut(s) 1202, 1586, 2296, 2518
SspMI CTAG 7 cut(s) 41, 477, 497, 905, 2337, 2382, 2454
SstI GAGCTC 2 cut(s) 144, 315
StyD4I CCNGG 7 cut(s) 116, 173, 200, 232, 916, 2215, 2537
StyI CCWWGG 4 cut(s) 514, 540, 586, 958
TaaI ACNGT 8 cut(s) 462, 706, 1680, 1751, 1978, 2134, 2677, 2712
TaiI ACGT 4 cut(s) 893, 997, 1378, 1767
TaqI TCGA 6 cut(s) 763, 979, 1347, 1368, 1484, 2317
TaqII GACCGA 1 cut(s) 356
TauI GCSGC 2 cut(s) 92, 2746
TfiI GAWTC 8 cut(s) 536, 573, 963, 1034, 1454, 1616, 2193, 2764
TscAI CASTG 8 cut(s) 334, 529, 669, 1743, 1756, 1819, 1969, 2352
TseI GCWGC 9 cut(s) 626, 629, 1094, 1333, 2024, 2244, 2402, 2594, 2825
TspDTI ATGAA 8 cut(s) 17, 1283, 1454, 1623, 1806, 1853, 2238, 2658
TspGWI ACGGA 2 cut(s) 659, 2807
TspRI CASTG 8 cut(s) 334, 529, 669, 1743, 1756, 1819, 1969, 2352
Vha464I CTTAAG 3 cut(s) 1202, 2296, 2518
VneI GTGCAC 1 cut(s) 407
VpaK11BI GGWCC 4 cut(s) 105, 177, 229, 920
VspI ATTAAT 1 cut(s) 869
XapI RAATTY 6 cut(s) 503, 598, 721, 1632, 2001, 2099
XceI RCATGY 1 cut(s) 916
XcmI CCANNNNNNNNNTGG 2 cut(s) 2213, 2406
XmnI GAANNNNTTC 1 cut(s) 461
XspI CTAG 7 cut(s) 41, 477, 497, 905, 2337, 2382, 2454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.