pycom03g11550

MIP18 family protein At1g68310-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009043
Physical Location & Seq
Forward (+)
11363338 .. 11365417
2080 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 438 bp
ATGCTGTACCTTAATCTGATCGAAAGTAGTTTTATGTTTTTCCTTGTATGCTGGGAAGAATTTAGTATTCCTTTACAATTCTTAAACCATATTAGAGATATAAAGGACCCGGAACACCCTTATTCGTTGGAGGAGCTCAAAGTGATAACAGAAGATGCAATTGAAGTTGATGATGGTCGTGGCTACGTCAGGGTCACATTTACTCCTACGGTGGAACATTGTAGTATGGCAACGGTTATTGGTCTTTGCTTACGTGTTAAACTCTTAAGGAGCCTACCTTCTCGTTACAAGGTGGATATTAGGGTGGCACCCGGATCTCATGCAACCGAAGCTGCAGTTAATAAGCAACTGAATGATAAGGAACGGGTGGCTGCGGCGTTGGAGAACCCAGGCCTCGTTGATATGGTTGATGAATGCCTTTCTCCTTCGTATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FeS_assembly_P

Protein Analysis

146

Amino Acids

16.47

Weight (kDa)

4.89

Isoelectric Point (pI)

48.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeS_assembly_P PF01883 29 - 98 3.2e-07 Iron-sulfur cluster assembly protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 307
AciI CCGC 1 cut(s) 374
AclWI GGATC 1 cut(s) 322
AcsI RAATTY 1 cut(s) 59
AfaI GTAC 1 cut(s) 8
AflII CTTAAG 1 cut(s) 265
AflIII ACRYGT 1 cut(s) 253
AgsI TTSAA 1 cut(s) 164
AjnI CCWGG 1 cut(s) 388
AluBI AGCT 2 cut(s) 136, 332
AluI AGCT 2 cut(s) 136, 332
Alw21I GWGCWC 1 cut(s) 138
AlwI GGATC 1 cut(s) 322
AoxI GGCC 1 cut(s) 391
ApeKI GCWGC 2 cut(s) 332, 371
ApoI RAATTY 1 cut(s) 59
AspS9I GGNCC 1 cut(s) 106
AsuC2I CCSGG 2 cut(s) 110, 312
AvaII GGWCC 1 cut(s) 106
BanI GGYRCC 1 cut(s) 307
BanII GRGCYC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 319, 358
BccI CCATC 1 cut(s) 167
BciT130I CCWGG 1 cut(s) 390
BcnI CCSGG 2 cut(s) 110, 312
BfmI CTRYAG 1 cut(s) 333
BfrI CTTAAG 1 cut(s) 265
BisI GCNGC 3 cut(s) 333, 372, 375
BlsI GCNGC 3 cut(s) 334, 373, 376
Bme1390I CCNGG 3 cut(s) 110, 312, 390
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 3 cut(s) 108, 272, 309
BmrFI CCNGG 3 cut(s) 110, 312, 390
BmsI GCATC 1 cut(s) 145
BpuMI CCSGG 2 cut(s) 110, 312
BsaAI YACGTR 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 388
BsaXI ACNNNNNCTCC 2 cut(s) 187, 217
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 1 cut(s) 388
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 2 cut(s) 319, 358
BseYI CCCAGC 1 cut(s) 51
BshFI GGCC 1 cut(s) 393
BshNI GGYRCC 1 cut(s) 307
BsiHKAI GWGCWC 1 cut(s) 138
BsiSI CCGG 2 cut(s) 110, 312
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 1 cut(s) 393
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 2 cut(s) 18, 314
BspACI CCGC 1 cut(s) 374
BspANI GGCC 1 cut(s) 393
BspLI GGNNCC 3 cut(s) 108, 272, 309
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 1 cut(s) 322
BspT107I GGYRCC 1 cut(s) 307
BspTI CTTAAG 1 cut(s) 265
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 2 cut(s) 18, 314
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 2 cut(s) 211, 235
BstAFI CTTAAG 1 cut(s) 265
BstBAI YACGTR 1 cut(s) 254
BstKTI GATC 2 cut(s) 21, 317
BstMBI GATC 2 cut(s) 18, 314
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 1 cut(s) 390
BstSCI CCNGG 3 cut(s) 108, 310, 388
BstSFI CTRYAG 1 cut(s) 333
BstV1I GCAGC 2 cut(s) 319, 358
BstX2I RGATCY 1 cut(s) 314
BstYI RGATCY 1 cut(s) 314
BsuRI GGCC 1 cut(s) 393
Cfr13I GGNCC 1 cut(s) 106
Csp6I GTAC 1 cut(s) 7
CviAII CATG 1 cut(s) 320
CviJI RGCY 6 cut(s) 136, 183, 273, 332, 371, 393
CviKI_1 RGCY 6 cut(s) 136, 183, 273, 332, 371, 393
CviQI GTAC 1 cut(s) 7
DpnI GATC 2 cut(s) 20, 316
DpnII GATC 2 cut(s) 18, 314
Ecl136II GAGCTC 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 393
Eco24I GRGCYC 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 106
Eco53kI GAGCTC 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 388
EcoT38I GRGCYC 1 cut(s) 138
FaeI CATG 1 cut(s) 323
FaiI YATR 8 cut(s) 35, 49, 90, 101, 227, 321, 404, 432
FatI CATG 1 cut(s) 319
Fnu4HI GCNGC 3 cut(s) 333, 372, 375
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 3 cut(s) 333, 372, 375
GluI GCNGC 3 cut(s) 333, 372, 375
GsaI CCCAGC 1 cut(s) 55
HaeIII GGCC 1 cut(s) 393
HapII CCGG 2 cut(s) 110, 312
Hin1II CATG 1 cut(s) 323
HpaII CCGG 2 cut(s) 110, 312
Hpy188I TCNGA 1 cut(s) 18
HpyAV CCTTC 2 cut(s) 288, 435
HpyCH4III ACNGT 2 cut(s) 211, 235
HpyCH4IV ACGT 2 cut(s) 186, 253
HpyCH4V TGCA 3 cut(s) 158, 323, 335
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpySE526I ACGT 2 cut(s) 186, 253
Hsp92II CATG 1 cut(s) 323
Kzo9I GATC 2 cut(s) 18, 314
LmnI GCTCC 2 cut(s) 133, 270
LpnPI CCDG 6 cut(s) 37, 123, 175, 325, 375, 402
Lsp1109I GCAGC 2 cut(s) 319, 358
LweI GCATC 1 cut(s) 145
MaeII ACGT 2 cut(s) 186, 253
MaeIII GTNAC 2 cut(s) 193, 284
MalI GATC 2 cut(s) 20, 316
MboI GATC 2 cut(s) 18, 314
MboII GAAGA 2 cut(s) 68, 164
MfeI CAATTG 1 cut(s) 159
MflI RGATCY 1 cut(s) 314
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 3 cut(s) 59, 77, 159
MmeI TCCRAC 2 cut(s) 108, 360
MnlI CCTC 2 cut(s) 124, 404
MseI TTAA 5 cut(s) 12, 83, 258, 266, 339
MspCI CTTAAG 1 cut(s) 265
MspI CCGG 2 cut(s) 110, 312
MspR9I CCNGG 3 cut(s) 110, 312, 390
MunI CAATTG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 419
MvaI CCWGG 1 cut(s) 390
MwoI GCNNNNNNNGC 1 cut(s) 329
NciI CCSGG 2 cut(s) 110, 312
NdeII GATC 2 cut(s) 18, 314
NlaIII CATG 1 cut(s) 323
NlaIV GGNNCC 3 cut(s) 108, 272, 309
NmuCI GTSAC 1 cut(s) 193
PceI AGGCCT 1 cut(s) 393
PctI GAATGC 1 cut(s) 419
PkrI GCNGC 3 cut(s) 334, 373, 376
Ppu21I YACGTR 1 cut(s) 254
PpuMI RGGWCCY 1 cut(s) 106
Psp124BI GAGCTC 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 106
Psp6I CCWGG 1 cut(s) 388
PspFI CCCAGC 1 cut(s) 51
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 3 cut(s) 108, 272, 309
PspPI GGNCC 1 cut(s) 106
PspPPI RGGWCCY 1 cut(s) 106
PstI CTGCAG 1 cut(s) 337
PsuI RGATCY 1 cut(s) 314
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 5 cut(s) 12, 83, 258, 266, 339
SatI GCNGC 3 cut(s) 333, 372, 375
Sau3AI GATC 2 cut(s) 18, 314
Sau96I GGNCC 1 cut(s) 106
ScrFI CCNGG 3 cut(s) 110, 312, 390
SduI GDGCHC 1 cut(s) 138
SetI ASST 7 cut(s) 12, 138, 189, 256, 280, 294, 334
SfaNI GCATC 1 cut(s) 145
SfcI CTRYAG 1 cut(s) 333
SinI GGWCC 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 265
SmoI CTYRAG 1 cut(s) 265
Sse9I AATT 3 cut(s) 59, 77, 159
SseBI AGGCCT 1 cut(s) 393
SsiI CCGC 1 cut(s) 374
SstI GAGCTC 1 cut(s) 138
StuI AGGCCT 1 cut(s) 393
StyD4I CCNGG 3 cut(s) 108, 310, 388
TaaI ACNGT 2 cut(s) 211, 235
TaiI ACGT 2 cut(s) 189, 256
TaqI TCGA 1 cut(s) 21
TasI AATT 3 cut(s) 59, 77, 159
TauI GCSGC 1 cut(s) 377
Tru1I TTAA 5 cut(s) 12, 83, 258, 266, 339
Tru9I TTAA 5 cut(s) 12, 83, 258, 266, 339
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 2 cut(s) 332, 371
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 1 cut(s) 426
Vha464I CTTAAG 1 cut(s) 265
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.