pycom03g15390

Nucleolar pre-ribosomal-associated protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002923
Physical Location & Seq
Forward (+)
16660267 .. 16666355
6089 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2427 bp
ATGCCTGGCATTCTAAGATCGCTTGTTGTCCCTTCCGCTAACCAAAGTGTAATTGATCACAAGTGCAATCTGGAAACAGTGGATCTGTATGCAAGACAGTTGGAGGTTGTCAAGTTACTACGGATGCTCTTTCCAATCAAGGAGCATCAACATAGTTTTGACTTTGGTAAAGATATATGTATAAATCTAAAAGAACTGCATTTACTGCTTCTGTCTTCTTATGGTGCAACACTTAGTGAAATTGATGTGGAGATATACAACTTGATACGCACAATTGAGTGTCTTGATGGTTCAAAGCCTGCCAATGTTGCTGGAATGGATCACTTGTGGGGCAGTGCCGTGTTAAAGATAGAAAAAGAACGGACTCTGGAAAGGAATATGTCTGATGATGTCTTGACTGATACTGAAGCGGTAAAAGAACGCCGTAGAAGTCAATTTAGAGAAAATCTTGTTGTTGACCCCAAAATCTGTGCGTCCACTGTGCTATATTTTCCTTATGATAGAGTGGCATCTGAAGAACTACTATGCTTGAACAAGTTTCAAACAAATAATTTTGATGATGGTTGTTTGTTGCATTCTCTTGATGCTGAATATATACAACGATATAATCCTATTTTCATCTTGCGCTTCTCTATTCATAGCCTCGCAGAGGGTTATTTTGAACCCTTGGAGTTTGCTGGTTTAGGCTTGCTAGCTATTGCATTTATGAGTATGTCATCACCTGACGACAGAATAAGAAGGTTGGGTTATGACACTCTTGGAAGATTTAAGAACACATTGGAGAGATGTCAAAAGAGGAAGGGTGTCAAGCACATTCAACTTCTGTTGACTTATGTGCAAAATGCTATAGAAGAACCATGGCAGAGAATCCCTTCTGTAAATTCTATTTTTGCTGCAGAGACATCCCTCATTTTATTAGATCCTTCACATGAGCATTATGCAGCCTTAACGACGCTTTTGATGGGATCGTCCAGGTTGAATGTCAAGAATATACTTTTCTTTAGCAATTTTTTTTGGAGTTCCTCTATCAATTTCAAAGCAGAAAGGTTGTGGATACTTCGCTTAGTATATGCAGGGCTGAATTTTGATGAGGATGCTCAAATTTATATGAACAACTCGGTTCTTGAGGCTCTGATGAGTTTCTATGTCTCTCCAATTTCGGAAGATGAATCCAAGGAACTAATACTTCAGGTAGTCAAGAAGGCAGTTAAATTACACAAGATGGCTCGTCATCTGGTTGAAAAATGTGGTCTTTTTTCGTGGTTATCATCTGTTCTCTCAGTATTGGGCGAATGGCATTTCAGAGATGGAAAAAGTTTGGTCTTGTTGCAGTTGGGTGTGGTATCAGAGGTCGTCAATGATGTTATTTCTTTGAGAAAAATCAATGAGTGGTTGCAACAATATGCCCTTGAGCAGCTTATGGAACTTGCATCTCATCTCTTCAAATTTCTTGTCAGTGATATGACACTGATAAAGGATAACGTTGCTGCTGTCAATCCAATTCTGGGAACAATAATATTAACACTCAAGATGTCCCAGAAGAGAAGGATATATCAGCCACATTTTGTTCTATCGGTTGAGGGTTCGTATAACATATATAAGGCTGTTAAGATATGTGATAATGCAAGATCTTGTGGTACAGTAGAGCTTGCTCTTAAAGCTATACTTATGAGTGCACCTCCAGCGGACATATTTTGTTTGAGCCGTGAAAAGATTTCAAGTTTTATTGTGTGGGCAGTTTCTGCTGCTTTGGAAGCAGACTCTGCAAAGATGCTTCAGCCTAAAGAATCTCACCTATGTTTAATTAGTATTCCAGAGGAAGAGCTGTACGAAAATTCTTTGATATCAAAGCTTCTACGTTGGCTCACTGCTTCTGTAATTCTTGGGAAGCTTGATTGGAAATCCAATGATTTGGACCCCGAGGTTTCAAAGAGGTTAAATTTAAAAACTCTTCAGACTTTAATGGAGCTTGTTGAAAGTGCATCTGGAAGAAGGAGCAAAAGCAGATATGGTTGTGAGGAGATTTTAGCTTCTGCAATTCTTTTCCTCCAACAGCTTGCAGGCACAAACTACCAAATGCTACCATCAGTTGCAGCTGCCCTTAGTCTTTTGCTTTACGATGGCTCAGTTTTCACAGGGTTCTTGTGTAAAAATGGAAGTGTTGTGAAATCATTGTGGTCGAAGATAGGTTGCCCTGCTGAAGCAAATCTCTCTTGGAGATGGGCATTTTACCAGCCGTGGAAGGATCTTACGCAGGGACAAACTGGTTCACAAAAGATGGAGGAACTCCATGCTTGTCACTCTCTTTTGGATATAGTTTCAAATGTGCTAGGCAAACGACCATCAGAATTGCTAGTCTCGTCAACATATGATGTAGATAGGTTTGGTGCATTTGAATGGGAAAGAAGTTTTATACAGACTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NopRA1 Npa1

Protein Analysis

809

Amino Acids

91.68

Weight (kDa)

6.19

Isoelectric Point (pI)

52.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NopRA1 PF16201 231 - 421 1.3e-49 Nucleolar pre-ribosomal-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 36, 410, 1685
AclI AACGTT 1 cut(s) 1482
AclWI GGATC 5 cut(s) 90, 327, 914, 973, 2253
AcsI RAATTY 6 cut(s) 880, 1081, 1101, 1445, 1834, 1939
AcuI CTGAAG 6 cut(s) 426, 534, 1172, 1760, 1937, 2220
AdeI CACNNNGTG 1 cut(s) 236
AfaI GTAC 2 cut(s) 1639, 1829
AfiI CCNNNNNNNGG 2 cut(s) 649, 1505
AjnI CCWGG 2 cut(s) 4, 971
Alw21I GWGCWC 1 cut(s) 1678
Alw26I GTCTC 3 cut(s) 893, 1153, 2362
Alw44I GTGCAC 1 cut(s) 1674
AlwI GGATC 5 cut(s) 90, 327, 914, 973, 2253
AlwNI CAGNNNCTG 2 cut(s) 1742, 1763
Ama87I CYCGRG 1 cut(s) 1919
ApaLI GTGCAC 1 cut(s) 1674
ApeKI GCWGC 7 cut(s) 893, 941, 1414, 1487, 1745, 2093, 2096
ApoI RAATTY 6 cut(s) 880, 1081, 1101, 1445, 1834, 1939
ArsI GACNNNNNNTTYG 2 cut(s) 1437, 1469
Asp700I GAANNNNTTC 1 cut(s) 871
AspLEI GCGC 1 cut(s) 627
AspS9I GGNCC 1 cut(s) 1915
AsuHPI GGTGA 2 cut(s) 711, 1784
AsuNHI GCTAGC 1 cut(s) 691
AvaI CYCGRG 1 cut(s) 1919
AvaII GGWCC 1 cut(s) 1915
BaeGI GKGCMC 1 cut(s) 1678
BbsI GAAGAC 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 1678
BbvI GCAGC 7 cut(s) 880, 953, 1426, 1474, 1732, 2083, 2105
BceAI ACGGC 4 cut(s) 323, 408, 1689, 2221
BciT130I CCWGG 2 cut(s) 6, 973
BciVI GTATCC 1 cut(s) 1047
BclI TGATCA 1 cut(s) 55
BcoDI GTCTC 3 cut(s) 893, 1153, 2362
BfaI CTAG 3 cut(s) 692, 2330, 2354
BfmI CTRYAG 2 cut(s) 846, 894
BfuI GTATCC 1 cut(s) 1047
BglII AGATCT 1 cut(s) 1628
BisI GCNGC 7 cut(s) 894, 942, 1415, 1488, 1746, 2094, 2097
BlsI GCNGC 7 cut(s) 895, 943, 1416, 1489, 1747, 2095, 2098
Bme1390I CCNGG 2 cut(s) 6, 973
Bme18I GGWCC 1 cut(s) 1915
BmeT110I CYCGRG 1 cut(s) 1919
BmgT120I GGNCC 1 cut(s) 1915
BmiI GGNNCC 1 cut(s) 1917
BmrFI CCNGG 2 cut(s) 6, 973
BmsI GCATC 8 cut(s) 114, 154, 518, 574, 1084, 1439, 1761, 1991
BmtI GCTAGC 1 cut(s) 695
BpiI GAAGAC 1 cut(s) 207
BplI GAGNNNNNCTC 2 cut(s) 1663, 1695
BpmI CTGGAG 1 cut(s) 1665
BpuEI CTTGAG 3 cut(s) 1145, 1430, 1511
BsaJI CCNNGG 5 cut(s) 666, 857, 1173, 1920, 2237
Bsc4I CCNNNNNNNGG 2 cut(s) 649, 1505
Bse1I ACTGG 1 cut(s) 2269
BseBI CCWGG 2 cut(s) 6, 973
BseDI CCNNGG 5 cut(s) 666, 857, 1173, 1920, 2237
BseGI GGATG 3 cut(s) 129, 902, 1099
BseLI CCNNNNNNNGG 2 cut(s) 649, 1505
BseMII CTCAG 3 cut(s) 1293, 2139, 2412
BseNI ACTGG 1 cut(s) 2269
BseRI GAGGAG 1 cut(s) 2033
BseSI GKGCMC 1 cut(s) 1678
BseXI GCAGC 7 cut(s) 880, 953, 1426, 1474, 1732, 2083, 2105
BsiHKAI GWGCWC 1 cut(s) 1678
BsiHKCI CYCGRG 1 cut(s) 1919
BslFI GGGAC 3 cut(s) 14, 1519, 2271
BslI CCNNNNNNNGG 2 cut(s) 649, 1505
BsmAI GTCTC 3 cut(s) 893, 1153, 2362
BsmFI GGGAC 3 cut(s) 14, 1519, 2271
BsmI GAATGC 2 cut(s) 9, 574
BsoBI CYCGRG 1 cut(s) 1919
Bsp1286I GDGCHC 1 cut(s) 1678
Bsp143I GATC 8 cut(s) 17, 55, 82, 319, 919, 965, 1628, 2245
Bsp19I CCATGG 1 cut(s) 857
BspACI CCGC 3 cut(s) 36, 410, 1685
BspCNI CTCAG 3 cut(s) 1292, 2138, 2413
BspLI GGNNCC 1 cut(s) 1917
BspMAI CTGCAG 1 cut(s) 898
BspOI GCTAGC 1 cut(s) 695
BspPI GGATC 5 cut(s) 90, 327, 914, 973, 2253
BspQI GCTCTTC 1 cut(s) 1815
BsrI ACTGG 1 cut(s) 2269
BssECI CCNNGG 5 cut(s) 666, 857, 1173, 1920, 2237
BssMI GATC 8 cut(s) 17, 55, 82, 319, 919, 965, 1628, 2245
BssT1I CCWWGG 3 cut(s) 666, 857, 1173
Bst2UI CCWGG 2 cut(s) 6, 973
Bst4CI ACNGT 4 cut(s) 79, 99, 481, 1642
Bst6I CTCTTC 4 cut(s) 1445, 1535, 1815, 1956
BstAPI GCANNNNNTGC 3 cut(s) 205, 1742, 1763
BstC8I GCNNGC 6 cut(s) 300, 689, 693, 1650, 2058, 2062
BstDEI CTNAG 7 cut(s) 14, 233, 1063, 1279, 2102, 2125, 2421
BstDSI CCRYGG 2 cut(s) 857, 2237
BstENI CCTNNNNNAGG 1 cut(s) 647
BstF5I GGATG 3 cut(s) 129, 902, 1099
BstHHI GCGC 1 cut(s) 627
BstKTI GATC 8 cut(s) 20, 58, 85, 322, 922, 968, 1631, 2248
BstMAI GTCTC 3 cut(s) 893, 1153, 2362
BstMBI GATC 8 cut(s) 17, 55, 82, 319, 919, 965, 1628, 2245
BstMWI GCNNNNNNNGC 7 cut(s) 205, 308, 1658, 1682, 1742, 1754, 1763
BstNI CCWGG 2 cut(s) 6, 973
BstSCI CCNGG 2 cut(s) 4, 971
BstSFI CTRYAG 2 cut(s) 846, 894
BstSLI GKGCMC 1 cut(s) 1678
BstV1I GCAGC 7 cut(s) 880, 953, 1426, 1474, 1732, 2083, 2105
BstV2I GAAGAC 1 cut(s) 207
BstX2I RGATCY 4 cut(s) 82, 919, 1628, 2245
BstXI CCANNNNNNTGG 1 cut(s) 1912
BstYI RGATCY 4 cut(s) 82, 919, 1628, 2245
BsuI GTATCC 1 cut(s) 1047
BtgI CCRYGG 2 cut(s) 857, 2237
BtsCI GGATG 3 cut(s) 129, 902, 1099
BtsI GCAGTG 2 cut(s) 340, 1866
BtsIMutI CAGTG 6 cut(s) 84, 340, 477, 1462, 1466, 1866
Cac8I GCNNGC 6 cut(s) 300, 689, 693, 1650, 2058, 2062
CaiI CAGNNNCTG 2 cut(s) 1742, 1763
CfoI GCGC 1 cut(s) 627
Cfr13I GGNCC 1 cut(s) 1915
CseI GACGC 2 cut(s) 462, 961
Csp6I GTAC 2 cut(s) 1638, 1828
CviAII CATG 3 cut(s) 858, 929, 2291
CviQI GTAC 2 cut(s) 1638, 1828
DdeI CTNAG 7 cut(s) 14, 233, 1063, 1279, 2102, 2125, 2421
DpnI GATC 8 cut(s) 19, 57, 84, 321, 921, 967, 1630, 2247
DpnII GATC 8 cut(s) 17, 55, 82, 319, 919, 965, 1628, 2245
DraI TTTAAA 1 cut(s) 1944
DraIII CACNNNGTG 1 cut(s) 236
Eam1104I CTCTTC 4 cut(s) 1445, 1535, 1815, 1956
EarI CTCTTC 4 cut(s) 1445, 1535, 1815, 1956
Eco130I CCWWGG 3 cut(s) 666, 857, 1173
Eco32I GATATC 1 cut(s) 1845
Eco47I GGWCC 1 cut(s) 1915
Eco57I CTGAAG 6 cut(s) 426, 534, 1172, 1760, 1937, 2220
Eco88I CYCGRG 1 cut(s) 1919
EcoNI CCTNNNNNAGG 1 cut(s) 647
EcoRII CCWGG 2 cut(s) 4, 971
EcoRV GATATC 1 cut(s) 1845
EcoT14I CCWWGG 3 cut(s) 666, 857, 1173
ErhI CCWWGG 3 cut(s) 666, 857, 1173
FaeI CATG 3 cut(s) 861, 932, 2294
FalI AAGNNNNNCTT 2 cut(s) 1650, 1682
FaqI GGGAC 3 cut(s) 14, 1519, 2271
FatI CATG 3 cut(s) 857, 928, 2290
FauNDI CATATG 1 cut(s) 2368
FbaI TGATCA 1 cut(s) 55
Fnu4HI GCNGC 7 cut(s) 894, 942, 1415, 1488, 1746, 2094, 2097
FokI GGATG 3 cut(s) 136, 889, 1106
Fsp4HI GCNGC 7 cut(s) 894, 942, 1415, 1488, 1746, 2094, 2097
FspBI CTAG 3 cut(s) 692, 2330, 2354
GlaI GCGC 1 cut(s) 626
GluI GCNGC 7 cut(s) 894, 942, 1415, 1488, 1746, 2094, 2097
GsuI CTGGAG 1 cut(s) 1665
HgaI GACGC 2 cut(s) 462, 961
HhaI GCGC 1 cut(s) 627
Hin1II CATG 3 cut(s) 861, 932, 2294
Hin6I GCGC 1 cut(s) 625
HinP1I GCGC 1 cut(s) 625
HincII GTYRAC 3 cut(s) 457, 828, 2364
HindII GTYRAC 3 cut(s) 457, 828, 2364
HindIII AAGCTT 2 cut(s) 1850, 1889
HinfI GANTC 5 cut(s) 364, 867, 1169, 1760, 1787
HphI GGTGA 2 cut(s) 711, 1784
Hpy166II GTNNAC 6 cut(s) 457, 477, 828, 1676, 2270, 2364
Hpy188I TCNGA 8 cut(s) 385, 514, 1134, 1162, 1304, 1348, 1956, 2347
Hpy8I GTNNAC 6 cut(s) 457, 477, 828, 1676, 2270, 2364
Hpy99I CGWCG 1 cut(s) 955
HpyAV CCTTC 9 cut(s) 42, 732, 793, 882, 933, 1195, 1539, 1986, 2236
HpyCH4III ACNGT 4 cut(s) 79, 99, 481, 1642
HpyCH4IV ACGT 2 cut(s) 1482, 1858
HpyF10VI GCNNNNNNNGC 7 cut(s) 205, 308, 1658, 1682, 1742, 1754, 1763
HpyF3I CTNAG 7 cut(s) 14, 233, 1063, 1279, 2102, 2125, 2421
HpySE526I ACGT 2 cut(s) 1482, 1858
Hsp92II CATG 3 cut(s) 861, 932, 2294
HspAI GCGC 1 cut(s) 625
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 8 cut(s) 17, 55, 82, 319, 919, 965, 1628, 2245
LguI GCTCTTC 1 cut(s) 1815
LmnI GCTCC 3 cut(s) 142, 1966, 1995
Lsp1109I GCAGC 7 cut(s) 880, 953, 1426, 1474, 1732, 2083, 2105
LweI GCATC 8 cut(s) 114, 154, 518, 574, 1084, 1439, 1761, 1991
MaeI CTAG 3 cut(s) 692, 2330, 2354
MaeII ACGT 2 cut(s) 1482, 1858
MaeIII GTNAC 2 cut(s) 114, 2297
MalI GATC 8 cut(s) 19, 57, 84, 321, 921, 967, 1630, 2247
MboI GATC 8 cut(s) 17, 55, 82, 319, 919, 965, 1628, 2245
MfeI CAATTG 1 cut(s) 273
MflI RGATCY 4 cut(s) 82, 919, 1628, 2245
MhlI GDGCHC 1 cut(s) 1678
MlyI GAGTC 2 cut(s) 358, 1754
MmeI TCCRAC 2 cut(s) 81, 2074
MroXI GAANNNNTTC 1 cut(s) 871
MslI CAYNNNNRTG 1 cut(s) 2395
MspA1I CMGCKG 2 cut(s) 1685, 2096
MspR9I CCNGG 2 cut(s) 6, 973
MunI CAATTG 1 cut(s) 273
Mva1269I GAATGC 2 cut(s) 9, 574
MvaI CCWGG 2 cut(s) 6, 973
MwoI GCNNNNNNNGC 7 cut(s) 205, 308, 1658, 1682, 1742, 1754, 1763
NcoI CCATGG 1 cut(s) 857
NdeI CATATG 1 cut(s) 2368
NdeII GATC 8 cut(s) 17, 55, 82, 319, 919, 965, 1628, 2245
NheI GCTAGC 1 cut(s) 691
NlaIII CATG 3 cut(s) 861, 932, 2294
NlaIV GGNNCC 1 cut(s) 1917
NmuCI GTSAC 1 cut(s) 2297
PciSI GCTCTTC 1 cut(s) 1815
PctI GAATGC 2 cut(s) 9, 574
PdmI GAANNNNTTC 1 cut(s) 871
PfeI GAWTC 3 cut(s) 867, 1169, 1787
PkrI GCNGC 7 cut(s) 895, 943, 1416, 1489, 1747, 2095, 2098
PleI GAGTC 2 cut(s) 358, 1754
PpsI GAGTC 2 cut(s) 358, 1754
Psp1406I AACGTT 1 cut(s) 1482
Psp6I CCWGG 2 cut(s) 4, 971
PspGI CCWGG 2 cut(s) 4, 971
PspN4I GGNNCC 1 cut(s) 1917
PspPI GGNCC 1 cut(s) 1915
PsrI GAACNNNNNNTAC 2 cut(s) 186, 218
PstI CTGCAG 1 cut(s) 898
PstNI CAGNNNCTG 2 cut(s) 1742, 1763
PsuI RGATCY 4 cut(s) 82, 919, 1628, 2245
PvuII CAGCTG 1 cut(s) 2096
RsaI GTAC 2 cut(s) 1639, 1829
RsaNI GTAC 2 cut(s) 1638, 1828
RseI CAYNNNNRTG 1 cut(s) 2395
SapI GCTCTTC 1 cut(s) 1815
SatI GCNGC 7 cut(s) 894, 942, 1415, 1488, 1746, 2094, 2097
Sau3AI GATC 8 cut(s) 17, 55, 82, 319, 919, 965, 1628, 2245
Sau96I GGNCC 1 cut(s) 1915
SchI GAGTC 2 cut(s) 358, 1754
ScrFI CCNGG 2 cut(s) 6, 973
SduI GDGCHC 1 cut(s) 1678
SfaNI GCATC 8 cut(s) 114, 154, 518, 574, 1084, 1439, 1761, 1991
SfcI CTRYAG 2 cut(s) 846, 894
SinI GGWCC 1 cut(s) 1915
SmiMI CAYNNNNRTG 1 cut(s) 2395
SmlI CTYRAG 3 cut(s) 1124, 1409, 1526
SmoI CTYRAG 3 cut(s) 1124, 1409, 1526
SsiI CCGC 3 cut(s) 36, 410, 1685
SspI AATATT 1 cut(s) 1518
SspMI CTAG 3 cut(s) 692, 2330, 2354
StyD4I CCNGG 2 cut(s) 4, 971
StyI CCWWGG 3 cut(s) 666, 857, 1173
TaaI ACNGT 4 cut(s) 79, 99, 481, 1642
TaiI ACGT 2 cut(s) 1485, 1861
TaqI TCGA 1 cut(s) 2180
TfiI GAWTC 3 cut(s) 867, 1169, 1787
TscAI CASTG 6 cut(s) 84, 340, 484, 1462, 1473, 1873
TseFI GTSAC 1 cut(s) 2297
TseI GCWGC 7 cut(s) 893, 941, 1414, 1487, 1745, 2093, 2096
Tsp45I GTSAC 1 cut(s) 2297
TspDTI ATGAA 4 cut(s) 607, 626, 1124, 1182
TspGWI ACGGA 2 cut(s) 136, 376
TspRI CASTG 6 cut(s) 84, 340, 484, 1462, 1473, 1873
VneI GTGCAC 1 cut(s) 1674
VpaK11BI GGWCC 1 cut(s) 1915
XagI CCTNNNNNAGG 1 cut(s) 647
XapI RAATTY 6 cut(s) 880, 1081, 1101, 1445, 1834, 1939
XmnI GAANNNNTTC 1 cut(s) 871
XspI CTAG 3 cut(s) 692, 2330, 2354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.