Rroxscaffold_1G00040450

Nucleolar pre-ribosomal-associated protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002923
Physical Location & Seq
Reverse (-)
58171884 .. 58179241
7358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00040450.1

Sequence Viewer

Length: 2859 bp
ATGGTGGAGGCAGACTATGACAGTGAAGTTATGGGAGAAGAATTGCCGAAAGTTGAGCTTAAGGAGAATCATGAAGCGAAACTGAAAGAACTATTGCATAAGATTACTTCTAATGAGATTAAATTATGCGCTGCCGGTGCGAAAGAGTTTATAAAGTTGCTGAAAGGTGATTCTGGAGGTGAATTGCTGTATTACTATGTGCAAAATTCAGCTCAGTGCTCGGAGCTTCTGGGTGCGTGGAATCTCCGGCGAGGGAAGCCGGGGATGTTTTATATACTGAAGTTGATTTCTGTTGTTTTGAGTCATCCTGATGGGGTTTATGAAGCAAATGATATGGGGAGGTTGGGGGTGAGTAGGGTGTTGGACAAGTTTGCGCGGTTGATTATTGAGCAACAGTTGGGAGATGTGCATAAGGAGTTGAAGAGTAGTGAAGCTAAGCGCCAGAAGGCGGCGCTCTTGCTTTTGGCTTCGATTGTGAGGCGTGGATCGGTGTTGGCGTCTGAGGTTGAAAAGAAGTTTGATTTCAAGCTGCAGGGGTTTTGCAAGTTGGGAGAGTATAAAATGAGGCCAAATGAGAAAAGGAGGAAGCTGTCATTGAGGAAGTTGTTTGTTGGGTTTGCAATGTCGTTTCTGGAGGTGGGAGCGCCGGGATTGTTGAGGTCGGTTATACGGCAGAAGGAAATGTATTCTTGTGTCCTCCGCTGGCTGGGGAATGATGATGAGGAGACAGTTATATATGTTCTGTCCACATTGCGGAACAGGATACTTGTTGATGGTTCATCGGTTCCCCCTGCCCTTCGGAGTGTTCTGTTTGGGAGTGGTACTTTAGAGCAATTGGCCAATATTTCTGGAAGGGAGAATGGTGGAGCTTCTGCAGAATTGGCATACAGGGTCTTAGTTATGGTTTGCACCGATCCATCTAATGGCCTAATGGCAGATCCAAAGAGACACCTGAAGGGCAATTTAAAGCGGCAAATAGACCTAATGAAGAAGCTTAAGGCGACCGAGATTGGCTATCACAGGGATCTGCTTTTGGCTGTTGTTAGTGGGAGACCGTCTCTTGGTGCAGCATACATGGAAGAGTTCCCTTACAATCTTGAAGATTATGCATCACCCAAATGGTTTTCTGTTGTTACTCTAGCAGCAAACTTGGTTTCCTCCGTGGGTAGCGGGCTCCCAGCCTTGTTTGACAATGTTGATGTGCAGGATGTCATGAAATGCTTGTATCCTCGCCAGTTCAACCGCTCCACGATCAATAAAGGGCTGCTTCACTCTGATTTTCTAGTGAAGCATGGAACTTTAAGGCATCTTTTAGAGGCACTCAAGTTGTTAGATTCCTTCCTTGTTGTTTTAAACTGTAGAGACCAACATGTTTTGGAATCTCTCAAGCAAGAATTTCAAAATGAAGTTCGAAGCTTGCTCCCAGATCCTCAACTTTTTAAGAGTCTACTCTCTTCTATGAGTAGCAACTCAAGAAAGAGGACAGCAGATCCGGAGAAGTTCCCAGAACATAGCCCGAAGAATGTGAAAAAATTGAAGACAGATTTTGGAAACAAGGACTCAGATATTGTTGTAGGCGGAATAAGTTTTGGTCCAGATACGTCCGAAAATGAAGATAGTCTTGTGAATGTTATGGTAGATCTTTGGGGTTTCGATCCTTGCTCCACACCTATTACTGCACTCAAGGATGCAGACTTATATTTCTACTGTAGGGTGCTCGATGCTTTCAAAATATATCTTCGGATAATGCCCACTGGCTTGGAGGGCTCATTTGAGTTCCTTATGAAGCTCCTAAGCACTCCTTTAGCATTACAAAATAATCTGCAGTGCTCTCTTTTGTCTTTGCTAATAGAATACATTGGATGGTCTCCAAGGAATAGAACACCAATTAAGACTCCACCCTTGATGTACAAACATCTACAGACATTGATGAAGTTGCTGATTTTTTCACCACTCAGTGACATAAAAGATCAAGCATACTGTCTCACACAAGCAGCTATGTTAAGTACTGGTGCTTTTGACAGAAACCAACATGAAATTGCATCATGGTTCTTGTTTATACCGGGTTTTGATAGAGGAAAATCTTCGGTTGAAGTCCTGGGGGTGGGAGTATTACAAAGCTTGGCTAATGCTGTCATCTCATTCTTATCTGATGCCGTTTCTACTACAGGGAAGAATTTCTTCAAGCATTGGGATCTTATTGTTAAGCGTGATAACTACCATTTGGAAACTGTTAAAGCTGCAGATGTGTCACCTCAGGCCCCTCTAATCTTACGGGTACTGCAGAATTGTCTGAGATTGCTTGATTCTGGATCTGGGACTTTTACGTTACCTGAGAAGACAATGATATCTACCTACGTGTGCAATACACTGAAATATATCTTGAAAACTCAGGTTGATGCACGGTTTCTATCTTCTATGATTTGCTCACTCTTGGTTGAGAGACTTGGTGACTGTTCTACCACTCATCAGTCTAGGGGCAACTGTGAATGGAGGCCGTTGAACAGTTTATTTTTGTTTTCCCAGCGTATATCTGACCGTGAAATTTGTTGCATTTCTTCTATTGACAATAAGGCTAAGCCTTCTGGTAGTTCTTTTGCACTAGCGCTTGAGGAAGTTGGAAGACTTGCAAGAGGTGGGAGTGATGGTGAAGTAGCTGGAATAACCAAAGCCTTCTTCTCCTCATTTATCTGTACAACGCCTGATGAAATATTAGCAAACTTTCCATCAGTTATGGCAATTTCACAATGCCTTCTTGGTCTTCCATTCACTCTCTTTTCATCAATTTTCTTTCTTGAACCCACCCTTCTCCCCAGAGTTTCTAAGTTTTGGCCTGAAGTTTTTTTTTCCTGGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NopRA1 Npa1

Protein Analysis

952

Amino Acids

106.39

Weight (kDa)

8.54

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Npa1 PF11707 73 - 376 2e-43 Ribosome 60S biogenesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
AccB7I CCANNNNNTGG 1 cut(s) 923
AccBSI CCGCTC 1 cut(s) 1245
AccI GTMKAC 1 cut(s) 1447
AccII CGCG 1 cut(s) 376
AccIII TCCGGA 1 cut(s) 1492
AciI CCGC 8 cut(s) 376, 449, 700, 754, 970, 1170, 1243, 1578
AclWI GGATC 9 cut(s) 493, 908, 932, 1032, 1421, 1484, 1649, 2202, 2320
AcoI YGGCCR 1 cut(s) 837
AcsI RAATTY 4 cut(s) 205, 1394, 2176, 2544
AcuI CTGAAG 3 cut(s) 299, 974, 2856
AcyI GRCGYC 1 cut(s) 497
AdeI CACNNNGTG 1 cut(s) 1958
AfaI GTAC 5 cut(s) 823, 1910, 2008, 2280, 2695
AfeI AGCGCT 1 cut(s) 2607
AfiI CCNNNNNNNGG 6 cut(s) 448, 706, 753, 923, 1061, 2104
AflII CTTAAG 2 cut(s) 59, 995
AflIII ACRYGT 2 cut(s) 1369, 2358
AjnI CCWGG 2 cut(s) 2097, 2849
AjuI GAANNNNNNNTTGG 2 cut(s) 2660, 2692
Alw21I GWGCWC 3 cut(s) 221, 1719, 1832
Alw26I GTCTC 8 cut(s) 719, 940, 1045, 1062, 1356, 1872, 1988, 2437
AlwI GGATC 9 cut(s) 493, 908, 932, 1032, 1421, 1484, 1649, 2202, 2320
Aor13HI TCCGGA 1 cut(s) 1492
Aor51HI AGCGCT 1 cut(s) 2607
AoxI GGCC 6 cut(s) 566, 837, 925, 2259, 2495, 2831
ApeKI GCWGC 7 cut(s) 131, 529, 1067, 1142, 1264, 1994, 2240
ApoI RAATTY 4 cut(s) 205, 1394, 2176, 2544
Asp700I GAANNNNTTC 3 cut(s) 2083, 2177, 2180
AspLEI GCGC 6 cut(s) 131, 376, 441, 454, 646, 2608
AspS9I GGNCC 2 cut(s) 1592, 2260
AsuC2I CCSGG 3 cut(s) 261, 648, 2064
AsuHPI GGTGA 8 cut(s) 179, 191, 361, 1104, 1941, 2244, 2462, 2660
AsuII TTCGAA 1 cut(s) 1411
AvaII GGWCC 1 cut(s) 1592
AxyI CCTNAGG 1 cut(s) 2256
BalI TGGCCA 1 cut(s) 839
BanII GRGCYC 2 cut(s) 1176, 1769
BbsI GAAGAC 4 cut(s) 1544, 2345, 2629, 2753
Bbv12I GWGCWC 3 cut(s) 221, 1719, 1832
BbvI GCAGC 7 cut(s) 118, 516, 1079, 1154, 1251, 2006, 2227
BccI CCATC 6 cut(s) 305, 767, 925, 1857, 2639, 2734
BceAI ACGGC 3 cut(s) 686, 2141, 2482
BciT130I CCWGG 2 cut(s) 2099, 2851
BciVI GTATCC 2 cut(s) 756, 1236
BcnI CCSGG 3 cut(s) 261, 648, 2064
BcoDI GTCTC 8 cut(s) 719, 940, 1045, 1062, 1356, 1872, 1988, 2437
BfaI CTAG 4 cut(s) 1139, 1283, 2475, 2603
BfmI CTRYAG 9 cut(s) 530, 873, 1357, 1708, 1823, 1919, 2166, 2241, 2282
BfoI RGCGCY 4 cut(s) 442, 455, 647, 2609
BfrI CTTAAG 2 cut(s) 59, 995
BfuI GTATCC 2 cut(s) 756, 1236
BglII AGATCT 1 cut(s) 1639
BisI GCNGC 9 cut(s) 132, 450, 530, 971, 1068, 1143, 1265, 1995, 2241
BlpI GCTNAGC 2 cut(s) 435, 2577
BlsI GCNGC 9 cut(s) 133, 451, 531, 972, 1069, 1144, 1266, 1996, 2242
BmcAI AGTACT 1 cut(s) 2008
Bme1390I CCNGG 5 cut(s) 261, 648, 2064, 2099, 2851
Bme18I GGWCC 1 cut(s) 1592
BmgT120I GGNCC 2 cut(s) 1592, 2260
BmiI GGNNCC 3 cut(s) 786, 1175, 2262
BmrFI CCNGG 5 cut(s) 261, 648, 2064, 2099, 2851
BmsI GCATC 7 cut(s) 1118, 1315, 1678, 1711, 2051, 2143, 2389
BpiI GAAGAC 4 cut(s) 1544, 2345, 2629, 2753
BplI GAGNNNNNCTC 2 cut(s) 1042, 1074
BpmI CTGGAG 2 cut(s) 195, 653
Bpu10I CCTNAGC 1 cut(s) 1793
Bpu1102I GCTNAGC 2 cut(s) 435, 2577
Bpu14I TTCGAA 1 cut(s) 1411
BpuEI CTTGAG 5 cut(s) 1307, 1370, 1456, 1667, 2630
BpuMI CCSGG 3 cut(s) 261, 648, 2064
BsaAI YACGTR 1 cut(s) 2359
BsaHI GRCGYC 1 cut(s) 497
BsaI GGTCTC 3 cut(s) 1045, 1356, 1872
BsaJI CCNNGG 5 cut(s) 260, 1161, 1871, 2098, 2850
BsaWI WCCGGW 1 cut(s) 1492
BsaXI ACNNNNNCTCC 2 cut(s) 2632, 2662
Bsc4I CCNNNNNNNGG 6 cut(s) 448, 706, 753, 923, 1061, 2104
Bse118I RCCGGY 1 cut(s) 134
Bse1I ACTGG 3 cut(s) 1234, 1759, 2014
Bse21I CCTNAGG 1 cut(s) 2256
Bse3DI GCAATG 2 cut(s) 627, 749
BseAI TCCGGA 1 cut(s) 1492
BseBI CCWGG 2 cut(s) 2099, 2851
BseDI CCNNGG 5 cut(s) 260, 1161, 1871, 2098, 2850
BseGI GGATG 5 cut(s) 270, 304, 1213, 1693, 1868
BseLI CCNNNNNNNGG 6 cut(s) 448, 706, 753, 923, 1061, 2104
BseMI GCAATG 2 cut(s) 627, 749
BseMII CTCAG 8 cut(s) 227, 492, 1575, 1969, 2270, 2285, 2325, 2405
BseNI ACTGG 3 cut(s) 1234, 1759, 2014
BseRI GAGGAG 2 cut(s) 737, 2671
BseXI GCAGC 7 cut(s) 118, 516, 1079, 1154, 1251, 2006, 2227
BseYI CCCAGC 3 cut(s) 706, 1177, 2523
BsgI GTGCAG 3 cut(s) 1086, 1223, 1662
Bsh1236I CGCG 1 cut(s) 376
Bsh1285I CGRYCG 1 cut(s) 1005
BshFI GGCC 6 cut(s) 568, 839, 927, 2261, 2497, 2833
BsiEI CGRYCG 1 cut(s) 1005
BsiHKAI GWGCWC 3 cut(s) 221, 1719, 1832
BsiSI CCGG 6 cut(s) 135, 247, 260, 647, 1493, 2063
BslFI GGGAC 1 cut(s) 2332
BslI CCNNNNNNNGG 6 cut(s) 448, 706, 753, 923, 1061, 2104
BsmAI GTCTC 8 cut(s) 719, 940, 1045, 1062, 1356, 1872, 1988, 2437
BsmBI CGTCTC 1 cut(s) 1062
BsmFI GGGAC 1 cut(s) 2332
BsnI GGCC 6 cut(s) 568, 839, 927, 2261, 2497, 2833
Bso31I GGTCTC 3 cut(s) 1045, 1356, 1872
Bsp119I TTCGAA 1 cut(s) 1411
Bsp1286I GDGCHC 5 cut(s) 221, 1176, 1719, 1769, 1832
Bsp13I TCCGGA 1 cut(s) 1492
Bsp1407I TGTACA 2 cut(s) 1908, 2693
Bsp1720I GCTNAGC 2 cut(s) 435, 2577
BspACI CCGC 8 cut(s) 376, 449, 700, 754, 970, 1170, 1243, 1578
BspANI GGCC 6 cut(s) 568, 839, 927, 2261, 2497, 2833
BspCNI CTCAG 8 cut(s) 226, 493, 1574, 1968, 2269, 2286, 2326, 2404
BspEI TCCGGA 1 cut(s) 1492
BspFNI CGCG 1 cut(s) 376
BspHI TCATGA 2 cut(s) 70, 1212
BspLI GGNNCC 3 cut(s) 786, 1175, 2262
BspMAI CTGCAG 5 cut(s) 534, 877, 1827, 2245, 2286
BspPI GGATC 9 cut(s) 493, 908, 932, 1032, 1421, 1484, 1649, 2202, 2320
BspT104I TTCGAA 1 cut(s) 1411
BspTI CTTAAG 2 cut(s) 59, 995
BspTNI GGTCTC 3 cut(s) 1045, 1356, 1872
BsrBI CCGCTC 1 cut(s) 1245
BsrDI GCAATG 2 cut(s) 627, 749
BsrFI RCCGGY 1 cut(s) 134
BsrGI TGTACA 2 cut(s) 1908, 2693
BsrI ACTGG 3 cut(s) 1234, 1759, 2014
BssAI RCCGGY 1 cut(s) 134
BssECI CCNNGG 5 cut(s) 260, 1161, 1871, 2098, 2850
BssNI GRCGYC 1 cut(s) 497
BssT1I CCWWGG 1 cut(s) 1871
Bst2UI CCWGG 2 cut(s) 2099, 2851
Bst6I CTCTTC 3 cut(s) 416, 1074, 1459
BstACI GRCGYC 1 cut(s) 497
BstAFI CTTAAG 2 cut(s) 59, 995
BstAUI TGTACA 2 cut(s) 1908, 2693
BstBAI YACGTR 1 cut(s) 2359
BstBI TTCGAA 1 cut(s) 1411
BstC8I GCNNGC 3 cut(s) 704, 1172, 1418
BstDSI CCRYGG 1 cut(s) 1161
BstF5I GGATG 5 cut(s) 270, 304, 1213, 1693, 1868
BstFNI CGCG 1 cut(s) 376
BstH2I RGCGCY 4 cut(s) 442, 455, 647, 2609
BstHHI GCGC 6 cut(s) 131, 376, 441, 454, 646, 2608
BstMAI GTCTC 8 cut(s) 719, 940, 1045, 1062, 1356, 1872, 1988, 2437
BstMCI CGRYCG 1 cut(s) 1005
BstMWI GCNNNNNNNGC 4 cut(s) 137, 256, 881, 1764
BstNI CCWGG 2 cut(s) 2099, 2851
BstNSI RCATGY 1 cut(s) 1373
BstSCI CCNGG 5 cut(s) 259, 646, 2062, 2097, 2849
BstSFI CTRYAG 9 cut(s) 530, 873, 1357, 1708, 1823, 1919, 2166, 2241, 2282
BstUI CGCG 1 cut(s) 376
BstV1I GCAGC 7 cut(s) 118, 516, 1079, 1154, 1251, 2006, 2227
BstV2I GAAGAC 4 cut(s) 1544, 2345, 2629, 2753
BstX2I RGATCY 7 cut(s) 937, 1024, 1426, 1489, 1639, 2194, 2312
BstXI CCANNNNNNTGG 1 cut(s) 1759
BstYI RGATCY 7 cut(s) 937, 1024, 1426, 1489, 1639, 2194, 2312
Bsu36I CCTNAGG 1 cut(s) 2256
BsuI GTATCC 2 cut(s) 756, 1236
BsuRI GGCC 6 cut(s) 568, 839, 927, 2261, 2497, 2833
BtgI CCRYGG 1 cut(s) 1161
BtsCI GGATG 5 cut(s) 270, 304, 1213, 1693, 1868
BtsI GCAGTG 1 cut(s) 1832
BtsIMutI CAGTG 6 cut(s) 28, 221, 1752, 1832, 1963, 2369
Cac8I GCNNGC 3 cut(s) 704, 1172, 1418
CciI TCATGA 2 cut(s) 70, 1212
CfoI GCGC 6 cut(s) 131, 376, 441, 454, 646, 2608
Cfr10I RCCGGY 1 cut(s) 134
Cfr13I GGNCC 2 cut(s) 1592, 2260
CseI GACGC 1 cut(s) 486
Csp6I GTAC 5 cut(s) 822, 1909, 2007, 2279, 2694
CviAII CATG 7 cut(s) 71, 1075, 1213, 1292, 1370, 2033, 2046
CviQI GTAC 5 cut(s) 822, 1909, 2007, 2279, 2694
DraI TTTAAA 2 cut(s) 966, 1353
DraIII CACNNNGTG 1 cut(s) 1958
EaeI YGGCCR 1 cut(s) 837
Eam1104I CTCTTC 3 cut(s) 416, 1074, 1459
EarI CTCTTC 3 cut(s) 416, 1074, 1459
EciI GGCGGA 1 cut(s) 1593
Eco130I CCWWGG 1 cut(s) 1871
Eco24I GRGCYC 2 cut(s) 1176, 1769
Eco31I GGTCTC 3 cut(s) 1045, 1356, 1872
Eco32I GATATC 1 cut(s) 2349
Eco47I GGWCC 1 cut(s) 1592
Eco47III AGCGCT 1 cut(s) 2607
Eco57I CTGAAG 3 cut(s) 299, 974, 2856
Eco81I CCTNAGG 1 cut(s) 2256
EcoO109I RGGNCCY 1 cut(s) 2260
EcoRII CCWGG 2 cut(s) 2097, 2849
EcoRV GATATC 1 cut(s) 2349
EcoT14I CCWWGG 1 cut(s) 1871
EcoT22I ATGCAT 1 cut(s) 1111
EcoT38I GRGCYC 2 cut(s) 1176, 1769
ErhI CCWWGG 1 cut(s) 1871
Esp3I CGTCTC 1 cut(s) 1062
FaeI CATG 7 cut(s) 74, 1078, 1216, 1295, 1373, 2036, 2049
FaqI GGGAC 1 cut(s) 2332
FatI CATG 7 cut(s) 70, 1074, 1212, 1291, 1369, 2032, 2045
FauI CCCGC 1 cut(s) 1163
FblI GTMKAC 1 cut(s) 1447
Fnu4HI GCNGC 9 cut(s) 132, 450, 530, 971, 1068, 1143, 1265, 1995, 2241
FokI GGATG 5 cut(s) 277, 291, 1220, 1700, 1875
FriOI GRGCYC 2 cut(s) 1176, 1769
Fsp4HI GCNGC 9 cut(s) 132, 450, 530, 971, 1068, 1143, 1265, 1995, 2241
FspBI CTAG 4 cut(s) 1139, 1283, 2475, 2603
GlaI GCGC 6 cut(s) 130, 375, 440, 453, 645, 2607
GluI GCNGC 9 cut(s) 132, 450, 530, 971, 1068, 1143, 1265, 1995, 2241
GsaI CCCAGC 3 cut(s) 710, 1181, 2527
GsuI CTGGAG 2 cut(s) 195, 653
HaeII RGCGCY 4 cut(s) 442, 455, 647, 2609
HaeIII GGCC 6 cut(s) 568, 839, 927, 2261, 2497, 2833
HapII CCGG 6 cut(s) 135, 247, 260, 647, 1493, 2063
HgaI GACGC 1 cut(s) 486
HhaI GCGC 6 cut(s) 131, 376, 441, 454, 646, 2608
Hin1I GRCGYC 1 cut(s) 497
Hin1II CATG 7 cut(s) 74, 1078, 1216, 1295, 1373, 2036, 2049
Hin6I GCGC 6 cut(s) 129, 374, 439, 452, 644, 2606
HinP1I GCGC 6 cut(s) 129, 374, 439, 452, 644, 2606
HindIII AAGCTT 3 cut(s) 992, 1414, 2119
HpaII CCGG 6 cut(s) 135, 247, 260, 647, 1493, 2063
HphI GGTGA 8 cut(s) 179, 191, 361, 1104, 1941, 2244, 2462, 2660
Hpy166II GTNNAC 2 cut(s) 747, 1448
Hpy8I GTNNAC 2 cut(s) 747, 1448
HpyCH4IV ACGT 3 cut(s) 1601, 2327, 2358
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 256, 881, 1764
HpySE526I ACGT 3 cut(s) 1601, 2327, 2358
Hsp92I GRCGYC 1 cut(s) 497
Hsp92II CATG 7 cut(s) 74, 1078, 1216, 1295, 1373, 2036, 2049
HspAI GCGC 6 cut(s) 129, 374, 439, 452, 644, 2606
Kpn2I TCCGGA 1 cut(s) 1492
LmnI GCTCC 8 cut(s) 223, 641, 866, 1179, 1250, 1425, 1667, 1794
Lsp1109I GCAGC 7 cut(s) 118, 516, 1079, 1154, 1251, 2006, 2227
LweI GCATC 7 cut(s) 1118, 1315, 1678, 1711, 2051, 2143, 2389
MaeI CTAG 4 cut(s) 1139, 1283, 2475, 2603
MaeII ACGT 3 cut(s) 1601, 2327, 2358
MaeIII GTNAC 5 cut(s) 1132, 1958, 2250, 2328, 2450
MbiI CCGCTC 1 cut(s) 1245
MfeI CAATTG 1 cut(s) 833
MflI RGATCY 7 cut(s) 937, 1024, 1426, 1489, 1639, 2194, 2312
MhlI GDGCHC 5 cut(s) 221, 1176, 1719, 1769, 1832
MlsI TGGCCA 1 cut(s) 839
MluNI TGGCCA 1 cut(s) 839
MlyI GAGTC 4 cut(s) 310, 1453, 1553, 1888
MmeI TCCRAC 2 cut(s) 342, 2599
Mox20I TGGCCA 1 cut(s) 839
Mph1103I ATGCAT 1 cut(s) 1111
MroI TCCGGA 1 cut(s) 1492
MroXI GAANNNNTTC 3 cut(s) 2083, 2177, 2180
MscI TGGCCA 1 cut(s) 839
MslI CAYNNNNRTG 2 cut(s) 309, 1117
Msp20I TGGCCA 1 cut(s) 839
MspA1I CMGCKG 1 cut(s) 702
MspCI CTTAAG 2 cut(s) 59, 995
MspI CCGG 6 cut(s) 135, 247, 260, 647, 1493, 2063
MspR9I CCNGG 5 cut(s) 261, 648, 2064, 2099, 2851
MunI CAATTG 1 cut(s) 833
MvaI CCWGG 2 cut(s) 2099, 2851
MvnI CGCG 1 cut(s) 376
MwoI GCNNNNNNNGC 4 cut(s) 137, 256, 881, 1764
NciI CCSGG 3 cut(s) 261, 648, 2064
NlaIII CATG 7 cut(s) 74, 1078, 1216, 1295, 1373, 2036, 2049
NlaIV GGNNCC 3 cut(s) 786, 1175, 2262
NmuCI GTSAC 3 cut(s) 1958, 2250, 2450
NsiI ATGCAT 1 cut(s) 1111
NspI RCATGY 1 cut(s) 1373
NspV TTCGAA 1 cut(s) 1411
PagI TCATGA 2 cut(s) 70, 1212
PciI ACATGT 1 cut(s) 1369
PcsI WCGNNNNNNNCGW 1 cut(s) 494
PdmI GAANNNNTTC 3 cut(s) 2083, 2177, 2180
PfeI GAWTC 6 cut(s) 67, 170, 241, 1334, 1379, 2306
PflMI CCANNNNNTGG 1 cut(s) 923
PkrI GCNGC 9 cut(s) 133, 451, 531, 972, 1069, 1144, 1266, 1996, 2242
PleI GAGTC 4 cut(s) 309, 1452, 1553, 1888
PpsI GAGTC 4 cut(s) 309, 1452, 1553, 1888
Ppu21I YACGTR 1 cut(s) 2359
PscI ACATGT 1 cut(s) 1369
PsiI TTATAA 1 cut(s) 152
Psp6I CCWGG 2 cut(s) 2097, 2849
PspFI CCCAGC 3 cut(s) 706, 1177, 2523
PspGI CCWGG 2 cut(s) 2097, 2849
PspN4I GGNNCC 3 cut(s) 786, 1175, 2262
PspPI GGNCC 2 cut(s) 1592, 2260
PstI CTGCAG 5 cut(s) 534, 877, 1827, 2245, 2286
PsuI RGATCY 7 cut(s) 937, 1024, 1426, 1489, 1639, 2194, 2312
RsaI GTAC 5 cut(s) 823, 1910, 2008, 2280, 2695
RsaNI GTAC 5 cut(s) 822, 1909, 2007, 2279, 2694
RseI CAYNNNNRTG 2 cut(s) 309, 1117
SatI GCNGC 9 cut(s) 132, 450, 530, 971, 1068, 1143, 1265, 1995, 2241
Sau96I GGNCC 2 cut(s) 1592, 2260
ScaI AGTACT 1 cut(s) 2008
SchI GAGTC 4 cut(s) 310, 1453, 1553, 1888
ScrFI CCNGG 5 cut(s) 261, 648, 2064, 2099, 2851
SduI GDGCHC 5 cut(s) 221, 1176, 1719, 1769, 1832
SfaNI GCATC 7 cut(s) 1118, 1315, 1678, 1711, 2051, 2143, 2389
SfcI CTRYAG 9 cut(s) 530, 873, 1357, 1708, 1823, 1919, 2166, 2241, 2282
SfuI TTCGAA 1 cut(s) 1411
SinI GGWCC 1 cut(s) 1592
SmiMI CAYNNNNRTG 2 cut(s) 309, 1117
SmlI CTYRAG 7 cut(s) 59, 995, 1322, 1385, 1471, 1682, 2609
SmoI CTYRAG 7 cut(s) 59, 995, 1322, 1385, 1471, 1682, 2609
SsiI CCGC 8 cut(s) 376, 449, 700, 754, 970, 1170, 1243, 1578
SspI AATATT 2 cut(s) 844, 2712
SspMI CTAG 4 cut(s) 1139, 1283, 2475, 2603
StyD4I CCNGG 5 cut(s) 259, 646, 2062, 2097, 2849
StyI CCWWGG 1 cut(s) 1871
TaiI ACGT 3 cut(s) 1604, 2330, 2361
TaqI TCGA 4 cut(s) 470, 1411, 1653, 1719
TaqII GACCGA 1 cut(s) 1019
TatI WGTACW 3 cut(s) 1908, 2006, 2693
TauI GCSGC 2 cut(s) 452, 973
TfiI GAWTC 6 cut(s) 67, 170, 241, 1334, 1379, 2306
TscAI CASTG 6 cut(s) 28, 221, 1759, 1832, 1963, 2376
TseFI GTSAC 3 cut(s) 1958, 2250, 2450
TseI GCWGC 7 cut(s) 131, 529, 1067, 1142, 1264, 1994, 2240
Tsp45I GTSAC 3 cut(s) 1958, 2250, 2450
TspGWI ACGGA 1 cut(s) 1150
TspRI CASTG 6 cut(s) 28, 221, 1759, 1832, 1963, 2376
Van91I CCANNNNNTGG 1 cut(s) 923
Vha464I CTTAAG 2 cut(s) 59, 995
VpaK11BI GGWCC 1 cut(s) 1592
XapI RAATTY 4 cut(s) 205, 1394, 2176, 2544
XceI RCATGY 1 cut(s) 1373
XmiI GTMKAC 1 cut(s) 1447
XmnI GAANNNNTTC 3 cut(s) 2083, 2177, 2180
XspI CTAG 4 cut(s) 1139, 1283, 2475, 2603
ZrmI AGTACT 1 cut(s) 2008
Zsp2I ATGCAT 1 cut(s) 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.