Rw5G024750

Nucleolar pre-ribosomal-associated protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002923
Physical Location & Seq
Reverse (-)
35124177 .. 35137618
13442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G024750.1

Sequence Viewer

Length: 6774 bp
ATGGTTTGCACCGATCCATCTAATGGCCTAATGGCAGATTCAAAGAGACATCTGAAGGGCAATTTAAAGCGGCAAATAGACCTAATGAAGAAGCTTAAGGCGACCGAGATTGGCTACCACAGGGATCTGCTTTTGGCTGTTGTTAGCGGGAGACCGTCTCTTGGTGCAGCATACATGGAAGAGTTCCCTTACAATCTTGAAGATTATGCATCACCCAAATGGTTTTCTGTTGTTACTCTAGCAGCAAACTTGGTTTCCTCTGTGGGCAGTGGGATCCCAGCCTTGTTTGACAATGTTCATGTGCAGGATGTCATGAAATGCTTGTATCCTCGTCAGTTCAACCGCTCCATGATCAATAAAGGGCTGCTTCACTCTGATTTTCTAGTGAAGCATGGAACTTTAAGGCATCTTTTAGAGGCACTCAAGTTGTTAGATTCCTTCCTTGGTGTTTTAAACTGTAGAGACCAACATGTTTCGGAATCTCTCAAGCAAGAATTTCAAAATGAAGTTCGAAGCTTGCTCCCAGATCCTCAACTTTTTAAGACTCTACTCTCTTCTATGAGTAGCAACTCAAGAAAGAGGACTGCGGATCTGGAGAAGTTCCCAGAACATAGCCCGAAGAAAGTGAAAAAATTGAAAACAGATTTTGGAAACAAGGACTCAGATATTGTTGTAGGCGGAATAAGTTTTGGTCCAGATACGTCCGAAAATGAAGATAGTCTTGTGAATGTTATGGCAGATCTTTGGGGTTTCGATCCTTGCTCCACACCTATTACTGCACTCAAGGATGCAGACTTATATTTCTACTGTAGGGTGCTCGATGCTTTCAAAATATATCTTCGGATAATGCCCACTGGCTTGGAGGGCTCATTTGAGTTCCTTATGAAGCTCCTAAGCAGTCCTTTAGCATTACAAAATAATCTGCAGTGCTCTCTTTTGTCTTTGCTAATAGAATACATTGGATGGTCTCCAAGGAATAGAACACCAATTAAGACTCCACCCTTGATGTACAAACATCTACAGACATTGATGAAGTTGCTGATTTTTTCACCACTCAGTGACATAAAAGATCAAGCATACTGTCTCACACAAGCAGCTATGTTAAGTACTGGTGCTTTTGACAAAAACCAACATGAAATTGCATCATGGTTCTTGTTTATACCGGGTTTTGATAGAGGAAAATCTTCGGTTGAAGTCCTGGGGGTGGGAGTATTACAAAGCTTGGCTAATGCTGTCATCTCATTCTTATCTGATGCCGTTTCTACCACAGGGAAGAATTTCTTCAAGCATTGGGATCTTATTGTTAAGCGTGATAACTACCATTTGGAAACTGTTAAAGCTGCAGATGTGTCACCTCAGGCCCCTCTAATCTTACGGGTACTGCAGAATTGTCTGAGATTGCTTGATTCTGGATCTGGGACTTTTACATTACCTGAGAAGACAATGATATCTACCTACGTGTGCAATACACTGAAATATATCTTGAAAACTCAGGTTGATGCACAGTTTCTATCTTCTATGATTTGCTCAGTCTTGGTTGAGAGACTTGGTGACTGTTCTGCCACTCATCAATCTAGGGGCAACTGTGAATGGAGGCCGTTGAACAGTTTATTTTTGTTTTCCCAGAGTATATCTGACCGTGAAATTTGTTGCATTTCTTCTATTGACAATAAGGCTAAGCCTTCTGGTAGTTCTTTTGCACTAGCGCTTGAGGAAGTTGGAAGACTTGCAAGAGGTGGGAGTGATGGTGAAGTAGCTGGAATAACCAAAGCCTTCTTCTCCTCATTTATCTGTACAACGCCTGATGAAATATTAGCAAACTTTCCATCAGTTATGGCAATTTCACAATGCCTTGGTCTTCCATTCACTCTCTTTTCATCAATTTTCTTTCTTGAACCCACCCTTCTCCCCAGAGTTTCTAAGTTTTGGCCTGAAGTTTTTTTTCCTGGGCTTAGTATGGCTCTAACCAATATCTGTTGCAAAGGAGGAAAAGAAATTGCCTGTGGATCTCCTGATCATGCTTCTTATACAGGAGAAGCAATATGTAACCAAAATTGTGGTGCAAATGAAGCTGCTGCTACTGCCTTCAGTTTATTTTTGATGAAGGCACCTTTCCACGTTTTGTTCCCTGCCATTATGTGCATTGACGGTCCTTATTCGTTAGAGCCCTCAAAGATACAAGACCTGCTTTTGGCTAAACTATCAGACTCTGCAACTGATTACCACCTTATTTCCTATTTGCGCCTTGTGTTATTTTGGCTTTATCAGATACGGTTGTCTTGTACAGTTGAACAATTAGTCGGTTTCCAACAACTTTCTGAGATTTGCTCAGTTCTGGTGAAGAACTTATTATCTCAACTATTGGTGGCTGATTCTGATTCCCTCAGAACCTCCAGTGTAAATCTATCAAGTCATGATATTCAAAAGGTGGCTGAAACTATCTTTTATCATCCTGCAATAACTACATCGTTATCCTGCCCTTTGGAGTGTAGCGAAGACTTACCAGAGGGTAACCTACCAGACAACGTGGATGCTCTCATCAATTTATCGAGGGGGAAAGTTCATAGATTAGACCATCATGCCTTGGATATATTGGCAACAACTTGCAAGTACTTGTTTTCTCTGTGCAATGACAACCAATTTACACTTGAAGTAGAAGATGGGGATGCTACAAAGTTTGTGAAATCATTCAACGTCTTGGTTCATAAGCTCTTTCAGGAAGTCAAGGTCAAATTTGATCACAGCATTAGAAGTAACAATTGTATGGCTTTCCTCCCAACATTTTATGCTTTGCATGCTTTGAGTGGTTTTATATCTCCATTTGAGCTTCTTGAATTAGTGCGTTGGATGCTTAGTAGAGTTGATTTGGATGGCAACCAGAAATCTGCTATTTGTTTTGGATCTTGTATTGCTGGTGGTGCATTTAGAAATCTATCTAATTATTTGCAGCAGCCAAATACAAAGAGAAAGGCATTTGATTTGTTTTGGACAATGGAAGAAAACAATATTAGTAGCGACATTGTTGAAAAAATCTATATTAAGATATCAAAGCTTGCCTTTCTTTTGGAATCAGAAGTTGTAGATCATTGCTTGCTGGAAGCTATGAATGTGGTATACAGGCAGAAATGTATGCAAAAGTGTAATTTTCATCCATTAATAATAGTGATGTCGAGAGTTATAGCAACCACGCCAATAGAAATGCTTTCCCATTGCATTTACAGAACAAGCAAGATGAAAGCTAGATTGTTGTCTCTTCTTATTGATCTGAGCTCTATGCATTTGTCTACCTTTGGGCACTTACTTTTTGGTACTGTAGATACTAGTTGTCTACATAATGGCAATGTGACATCTGACCTAGCTCTTTCAGATGACGACTATATGATGCTTCTGCCTTCTGCTGTGTCCTACATGAATTCAGTTTTAATGAAATTTGGAGAGCCATGCTACAGGCATTTTAGAAATATTCCTTCCTTTTATTCAAAGATTATTTTGGATGGTCTCCTTCATTGGAAGAATTTTGTTTCTCGTGATGTGTTTCATGAAGATTTTGGAGAGGTTTTGCCGTCATCTGCTGAAGAAGTTCTTAACCTCATTAATGATAGTCTTCTTGGCAAAGCGATTTGTATGCTGCGCTATCACTATGCTTTGAATGCAGATTCAGTAGAAAGGAAAGACCGATTAAAGCATTTCAGTTCCATTTTCCCAGCTTCTGTGGGACACAAGGAGCTGATAGACTGGGATGTTGGTGGATTGGGTTCTCACTCTTTCAATGAAACATTAAACCTTGTAAATAAAATTCATGCAAAGGTCTCGTTTTGTAGGATGCTGTTATTCCCCAAAGATACTCAGGTTCACTCGCTTTCAATTGAAGCCAGTGGGAACCTGAAAGACGTTCCTCTGGACATGGAAGATAACAAAGGTAACTCCTCACAAATGCAGTTTTTGAACATCTTGGTGGATATGTGGCAATGCATTGTTAAAACAGTTCCTTCAGTCTTTCATAGTTCTCAAAAGGGACACAGCACAGATAGTTCTTCATTGTGGAGATACCTGGAAGTTCTGATATTGAAAAGCATTTTTGAAGTGTCTAGAGAGATGCATGACAGTCTAATCAGAGTACAATCTATACCCTTCCTAGAGAAATTGATGAAACCAGCTCTTCTGCATAGGTTTGACGATCCAACAACTTTGCAAATGCTGCGTGATCTTTTGACTTTTCTATCTGGGGGGAAATTTTCGCGTGTTCCCTATCTTCAGCTTTTACTTGCACACTCTCAATTTGTGCCTACTATTCGTTCACTATCCAAACCATCACATTCTTCCCATGTGGGTGCCTTTTCTAGGCCTATGTCTAGCATTCTTAGATCACCTGTTTCCCTTACCTCAAAACAAAATGTAGATGATGGGGAATGCGATCTAGAAACATCTGAGCTGTACGTTAAACAGTTGGAGGTTATCAAATTACTGCGGACACTCCTTCCTTTGAGGTTTCAACAGGATGGTTTTGACTTTGGAAAAGATTTAGGCGTTGATCTTAGGGAAGTTCATTTACTACTTTTGTCTTCTTATGGTGCAACACTTTCTGAAATTGATGTGGAGATATGCAATCTGATGCGCACAATTGAGTGTATTGATGGTTTGGAGCATGTCCGATTTGCTGGAATGGATTACCCTTGGGGCAGGGCTGCGTTGAAGATAGAAAAGGAACGGAATCTGCAGAGTTTGTCTTATGATACAATCAATGATGCTGAAGCAGTAAAAGAACACCGTAGAAATCAAGTTAGAGAAAATCTTGCGATTGACCCCAAAATATGCGCGTCCACTTTGCTATATTTTCCCTACCATATAGTGGCATCTGATGAGCTATTATCCTTGAACAAGTTTCAAACAGATCCTGTTGATCATATGCATGTGTTATGCTCCCCCGATGTTGACGCTAAAGAAAGATATAATCCTATTTTCATCTTGCGCTTCTCTATGCATTGTCTTTCAGAAGGTTACATTGAACCCTTGGAGTTTGCTGGTTTAGGCTTACTTGCTATTGCCTTTATGAGTATTTCATCACCTAATGACAAGATAAGAAGTTTGGGTTATGAAACTCTTGAAACATTGCAGGACAAGTTGAAGACATGTCAAAAGAAGAAGGGTGTCACGCAAATTAAGCTTCTGTTGCTTTATGTGGAAAATGGTATACAACAAATAGGGCAGAGAATCTCTTCTGTAAATGCTATTTTTGCTGCAGAGACATCCCTCATATTATTAGATACTTCACATGAACATTATGCAACTTTACTAACGCTATTGAAGAGATCGTCAGCATTGAACACGAAGAATGTACCTTTCTTTAGCAATTTTTTCTGGAGTTCCTCTATTAACTTCAGATCAGAAAGGTTGTGGATACTGCGGTTATTATATGTGGGGCTGAACTTTGACGATGATGCACATGTTTATATCAAGAATTCCATTCTTGAGACTCTGTTGAGTTTCTATGTTTCTCCCCTTTCAGATAAGGAGTCCAAGGAACTAATTCTTCAGGTAGTGAAGAAGTCAGTTAAATTGCATAAGTTGGCTCGTCATCTAGTTGAAAAATGTGGTCTGTTTCCGTGGTTATCATCTCTTCTCTCGATATCCAGTGAAAGTCGTCTCGAAGATGAAACTTTGTGCTTGTTGCAGTTGAGTGTGGTATCAGAGGTGGTCAATGATGTTGTTTCTTCAAGAAACATTACTGAATGGTTGCAGAACATTGCCCTCGAGCAGCTCATGGAGTTCACATCTCATCTCTACAAATTTCTTGCGACTGATGTGACTTTGATAACAGATAATGTTGCAGTGGTCAATCAAATTCTTAAAACAATAATATCTACATTCAAGGTATCCCAAAAGAGGATGATATATCAGCCACATTTTGTTGTATCATTCGACGGTTTATATAGAATATATAAGGCGGTCAAGGTCTACAATGATGCAAGATCATGTGCTACTGTAGAGTTTGGCCTTAAAGCCATTCTTATGAGTGCCCCTCCAGCTTCCATCTTTTATGTGAGTGGTGAAAAGCTGTCAAGTTTTATCATGTGGGCAATTTCCAGTGCTGTAGAAGCAGACTCTGCAACTATGCTTCATTCCAATGAATCTCATCAATGTTTCACTACTATTCCAGAGGAAAAAGAGGTGTATAAGAATTCTTTGATTTCAAAACTATTGCGTTGGCTAACTGCAACTGTAATTCTTGGGAAGCTTGACTGGACATTCAGTGACGTGGATCCTGAGTTCTCGAAAAGTTTGAATTTAAAAAGTTTGCACTCTTTAATAACTCATGCTGACAAGGCATGTGGAGAAAGGGGCCGAAGCAGATATGGTTGTGAAGAGATCTTGGCATCAGCAATTCTCTACCTTCAACAGCTCTCTGGAACAAATTACGAAATGCTACCATCTGTCAATGCCGCCCTTAGTCTTCTCCTTTCCAATGCCTCCATTTGTGCAGGTTTCTTGCATGATAATGAAACTGTCCAATCACTTTGGCAGAACATACGTTGCCCTAATGAAGCTAATCCTGCCTGGAGATGGTCATTTGACCAGCCATGGAAGGATCCGATGTTGGAAGTCAGTGATTCCCAGAAGATGGAGGAACTCCATGCTTGTGAATCACTTTTAGTTATATTTTCAAATCTGCTTGGAAAACAATCATCAGAATTTCAGGTTTCATCAACACAGGATATAGATAGATATGGTGTATTTGAGTGGGAAAAAAGTATCATAACATCTCAGAAATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NopRA1 Npa1

Protein Analysis

2257

Amino Acids

253.92

Weight (kDa)

6.19

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NopRA1 PF16201 1679 - 1869 3.3e-44 Nucleolar pre-ribosomal-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 4616
Acc36I ACCTGC 2 cut(s) 2193, 6470
AccB1I GGYRCC 2 cut(s) 2107, 4331
AccB7I CCANNNNNTGG 3 cut(s) 23, 4849, 6619
AccBSI CCGCTC 1 cut(s) 345
AccI GTMKAC 5 cut(s) 3114, 3284, 3328, 5191, 5955
AccII CGCG 2 cut(s) 4240, 4817
AciI CCGC 9 cut(s) 70, 147, 343, 587, 678, 4468, 5404, 5945, 6441
AcuI CTGAAG 9 cut(s) 74, 1953, 2071, 3594, 3975, 4238, 4770, 5362, 5516
AdeI CACNNNGTG 1 cut(s) 1058
AfeI AGCGCT 1 cut(s) 1707
AfiI CCNNNNNNNGG 9 cut(s) 23, 161, 1204, 2191, 4341, 4849, 5883, 6561, 6619
AflII CTTAAG 1 cut(s) 95
AflIII ACRYGT 4 cut(s) 469, 1458, 5129, 5443
AhlI ACTAGT 1 cut(s) 3320
AjiI CACGTC 1 cut(s) 6256
AjnI CCWGG 4 cut(s) 1197, 1945, 4050, 6554
AjuI GAANNNNNNNTTGG 2 cut(s) 1760, 1792
AloI GAACNNNNNNTCC 2 cut(s) 6251, 6283
Alw21I GWGCWC 3 cut(s) 819, 932, 3272
AlwNI CAGNNNCTG 4 cut(s) 2210, 3710, 4895, 6104
Ama87I CYCGRG 1 cut(s) 5750
Aor51HI AGCGCT 1 cut(s) 1707
AoxI GGCC 7 cut(s) 25, 1359, 1595, 1928, 4343, 5992, 6340
AseI ATTAAT 2 cut(s) 3155, 3594
Asp700I GAANNNNTTC 7 cut(s) 1183, 1277, 1280, 2687, 3579, 5215, 5526
AspLEI GCGC 6 cut(s) 1708, 2244, 3633, 4617, 4817, 4971
AspS9I GGNCC 4 cut(s) 692, 1360, 2150, 6340
AsuC2I CCSGG 1 cut(s) 1164
AsuHPI GGTGA 9 cut(s) 204, 1041, 1344, 1562, 1760, 2350, 4359, 5055, 6059
AsuII TTCGAA 1 cut(s) 511
AvaI CYCGRG 1 cut(s) 5750
AvaII GGWCC 2 cut(s) 692, 2150
AxyI CCTNAGG 1 cut(s) 1356
BaeGI GKGCMC 2 cut(s) 3297, 6019
BaeI ACNNNNGTAYC 6 cut(s) 2261, 2294, 3834, 3867, 5390, 5423
BamHI GGATCC 3 cut(s) 273, 6259, 6586
BanI GGYRCC 2 cut(s) 2107, 4331
BanII GRGCYC 3 cut(s) 869, 2169, 3272
BarI GAAGNNNNNNTAC 2 cut(s) 4533, 4565
BauI CACGAG 1 cut(s) 3525
BbsI GAAGAC 8 cut(s) 1445, 1729, 1850, 2502, 3596, 4554, 5132, 6443
Bbv12I GWGCWC 3 cut(s) 819, 932, 3272
BceAI ACGGC 3 cut(s) 1241, 1582, 3547
BcgI CGANNNNNNTGC 4 cut(s) 3607, 3641, 3792, 3826
BciT130I CCWGG 4 cut(s) 1199, 1947, 4052, 6556
BciVI GTATCC 3 cut(s) 336, 5391, 5884
BclI TGATCA 4 cut(s) 351, 2014, 2737, 4900
BcnI CCSGG 1 cut(s) 1164
BcuI ACTAGT 1 cut(s) 3320
BfoI RGCGCY 1 cut(s) 1709
BfrI CTTAAG 1 cut(s) 95
BfuAI ACCTGC 2 cut(s) 2193, 6470
BfuI GTATCC 3 cut(s) 336, 5391, 5884
BglII AGATCT 2 cut(s) 739, 6366
BlpI GCTNAGC 1 cut(s) 1677
BmcAI AGTACT 2 cut(s) 1108, 2612
Bme1390I CCNGG 5 cut(s) 1164, 1199, 1947, 4052, 6556
Bme18I GGWCC 2 cut(s) 692, 2150
BmeT110I CYCGRG 1 cut(s) 5750
BmgBI CACGTC 1 cut(s) 6256
BmgT120I GGNCC 4 cut(s) 692, 1360, 2150, 6340
BmiI GGNNCC 8 cut(s) 275, 1362, 2109, 3881, 4333, 6261, 6341, 6588
BmrFI CCNGG 5 cut(s) 1164, 1199, 1947, 4052, 6556
BmrI ACTGGG 1 cut(s) 3745
BmuI ACTGGG 1 cut(s) 3745
BpiI GAAGAC 8 cut(s) 1445, 1729, 1850, 2502, 3596, 4554, 5132, 6443
BplI GAGNNNNNCTC 6 cut(s) 142, 174, 2312, 2344, 6004, 6036
BpmI CTGGAG 5 cut(s) 614, 2377, 5380, 6006, 6577
Bpu10I CCTNAGC 1 cut(s) 893
Bpu1102I GCTNAGC 1 cut(s) 1677
Bpu14I TTCGAA 1 cut(s) 511
BpuEI CTTGAG 6 cut(s) 407, 470, 556, 767, 1730, 5489
BpuMI CCSGG 1 cut(s) 1164
BsaAI YACGTR 1 cut(s) 1459
BsaI GGTCTC 5 cut(s) 145, 456, 972, 3503, 3814
BsaXI ACNNNNNCTCC 2 cut(s) 1732, 1762
Bsc4I CCNNNNNNNGG 9 cut(s) 23, 161, 1204, 2191, 4341, 4849, 5883, 6561, 6619
Bse1I ACTGG 8 cut(s) 859, 1114, 2394, 3740, 3873, 5631, 6084, 6245
Bse21I CCTNAGG 1 cut(s) 1356
Bse3DI GCAATG 7 cut(s) 2635, 3085, 3208, 3346, 3974, 5108, 5742
BseBI CCWGG 4 cut(s) 1199, 1947, 4052, 6556
BseLI CCNNNNNNNGG 9 cut(s) 23, 161, 1204, 2191, 4341, 4849, 5883, 6561, 6619
BseMI GCAATG 7 cut(s) 2635, 3085, 3208, 3346, 3974, 5108, 5742
BseNI ACTGG 8 cut(s) 859, 1114, 2394, 3740, 3873, 5631, 6084, 6245
BseRI GAGGAG 2 cut(s) 1771, 3916
BseSI GKGCMC 2 cut(s) 3297, 6019
BseYI CCCAGC 2 cut(s) 277, 3703
BsgI GTGCAG 4 cut(s) 186, 323, 762, 6498
Bsh1236I CGCG 2 cut(s) 4240, 4817
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 7 cut(s) 27, 1361, 1597, 1930, 4345, 5994, 6342
BshNI GGYRCC 2 cut(s) 2107, 4331
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 3 cut(s) 819, 932, 3272
BsiHKCI CYCGRG 1 cut(s) 5750
BsiSI CCGG 1 cut(s) 1163
BslFI GGGAC 3 cut(s) 1432, 3729, 4029
BslI CCNNNNNNNGG 9 cut(s) 23, 161, 1204, 2191, 4341, 4849, 5883, 6561, 6619
BsmBI CGTCTC 2 cut(s) 162, 5648
BsmFI GGGAC 3 cut(s) 1432, 3729, 4029
BsmI GAATGC 3 cut(s) 3655, 4356, 4415
BsnI GGCC 7 cut(s) 27, 1361, 1597, 1930, 4345, 5994, 6342
Bso31I GGTCTC 5 cut(s) 145, 456, 972, 3503, 3814
BsoBI CYCGRG 1 cut(s) 5750
Bsp119I TTCGAA 1 cut(s) 511
Bsp1286I GDGCHC 7 cut(s) 819, 869, 932, 2169, 3272, 3297, 6019
Bsp1407I TGTACA 3 cut(s) 1008, 1793, 2282
Bsp1720I GCTNAGC 1 cut(s) 1677
Bsp19I CCATGG 1 cut(s) 6578
BspACI CCGC 9 cut(s) 70, 147, 343, 587, 678, 4468, 5404, 5945, 6441
BspANI GGCC 7 cut(s) 27, 1361, 1597, 1930, 4345, 5994, 6342
BspFNI CGCG 2 cut(s) 4240, 4817
BspHI TCATGA 3 cut(s) 312, 2413, 3538
BspLI GGNNCC 8 cut(s) 275, 1362, 2109, 3881, 4333, 6261, 6341, 6588
BspMAI CTGCAG 5 cut(s) 927, 1345, 1386, 4719, 5242
BspMI ACCTGC 2 cut(s) 2193, 6470
BspQI GCTCTTC 1 cut(s) 4164
BspT104I TTCGAA 1 cut(s) 511
BspT107I GGYRCC 2 cut(s) 2107, 4331
BspTI CTTAAG 1 cut(s) 95
BspTNI GGTCTC 5 cut(s) 145, 456, 972, 3503, 3814
BsrBI CCGCTC 1 cut(s) 345
BsrDI GCAATG 7 cut(s) 2635, 3085, 3208, 3346, 3974, 5108, 5742
BsrGI TGTACA 3 cut(s) 1008, 1793, 2282
BsrI ACTGG 8 cut(s) 859, 1114, 2394, 3740, 3873, 5631, 6084, 6245
BssNAI GTATAC 2 cut(s) 3115, 5192
BssSI CACGAG 1 cut(s) 3525
BssT1I CCWWGG 8 cut(s) 442, 971, 1852, 2583, 4673, 5010, 5517, 6578
Bst1107I GTATAC 2 cut(s) 3115, 5192
Bst2BI CACGAG 1 cut(s) 3525
Bst2UI CCWGG 4 cut(s) 1199, 1947, 4052, 6556
Bst6I CTCTTC 8 cut(s) 174, 559, 3258, 4164, 5221, 5300, 5622, 6357
BstAFI CTTAAG 1 cut(s) 95
BstAPI GCANNNNNTGC 2 cut(s) 4198, 6104
BstAUI TGTACA 3 cut(s) 1008, 1793, 2282
BstBAI YACGTR 1 cut(s) 1459
BstBI TTCGAA 1 cut(s) 511
BstC8I GCNNGC 4 cut(s) 518, 2796, 3054, 3092
BstDSI CCRYGG 2 cut(s) 5603, 6578
BstEII GGTNACC 1 cut(s) 2510
BstENI CCTNNNNNAGG 1 cut(s) 4339
BstFNI CGCG 2 cut(s) 4240, 4817
BstH2I RGCGCY 1 cut(s) 1709
BstHHI GCGC 6 cut(s) 1708, 2244, 3633, 4617, 4817, 4971
BstMCI CGRYCG 1 cut(s) 105
BstNI CCWGG 4 cut(s) 1199, 1947, 4052, 6556
BstNSI RCATGY 7 cut(s) 473, 2798, 4649, 4913, 5133, 5447, 6330
BstPI GGTNACC 1 cut(s) 2510
BstSCI CCNGG 5 cut(s) 1162, 1197, 1945, 4050, 6554
BstSLI GKGCMC 2 cut(s) 3297, 6019
BstUI CGCG 2 cut(s) 4240, 4817
BstV2I GAAGAC 8 cut(s) 1445, 1729, 1850, 2502, 3596, 4554, 5132, 6443
BstXI CCANNNNNNTGG 2 cut(s) 859, 2057
BstZ17I GTATAC 2 cut(s) 3115, 5192
Bsu36I CCTNAGG 1 cut(s) 1356
BsuI GTATCC 3 cut(s) 336, 5391, 5884
BsuRI GGCC 7 cut(s) 27, 1361, 1597, 1930, 4345, 5994, 6342
BtgI CCRYGG 2 cut(s) 5603, 6578
BtrI CACGTC 1 cut(s) 6256
BtsI GCAGTG 3 cut(s) 274, 932, 5835
BveI ACCTGC 2 cut(s) 2193, 6470
Cac8I GCNNGC 4 cut(s) 518, 2796, 3054, 3092
CaiI CAGNNNCTG 4 cut(s) 2210, 3710, 4895, 6104
CciI TCATGA 3 cut(s) 312, 2413, 3538
CfoI GCGC 6 cut(s) 1708, 2244, 3633, 4617, 4817, 4971
Cfr13I GGNCC 4 cut(s) 692, 1360, 2150, 6340
CseI GACGC 2 cut(s) 4806, 4943
CspCI CAANNNNNGTGG 2 cut(s) 6310, 6345
DraI TTTAAA 3 cut(s) 66, 453, 6288
DraIII CACNNNGTG 1 cut(s) 1058
Eam1104I CTCTTC 8 cut(s) 174, 559, 3258, 4164, 5221, 5300, 5622, 6357
EarI CTCTTC 8 cut(s) 174, 559, 3258, 4164, 5221, 5300, 5622, 6357
EciI GGCGGA 1 cut(s) 693
Ecl136II GAGCTC 1 cut(s) 3270
Eco130I CCWWGG 8 cut(s) 442, 971, 1852, 2583, 4673, 5010, 5517, 6578
Eco147I AGGCCT 1 cut(s) 4345
Eco24I GRGCYC 3 cut(s) 869, 2169, 3272
Eco31I GGTCTC 5 cut(s) 145, 456, 972, 3503, 3814
Eco32I GATATC 3 cut(s) 1449, 3045, 5628
Eco47I GGWCC 2 cut(s) 692, 2150
Eco47III AGCGCT 1 cut(s) 1707
Eco53kI GAGCTC 1 cut(s) 3270
Eco57I CTGAAG 9 cut(s) 74, 1953, 2071, 3594, 3975, 4238, 4770, 5362, 5516
Eco81I CCTNAGG 1 cut(s) 1356
Eco88I CYCGRG 1 cut(s) 5750
Eco91I GGTNACC 1 cut(s) 2510
EcoICRI GAGCTC 1 cut(s) 3270
EcoNI CCTNNNNNAGG 1 cut(s) 4339
EcoO109I RGGNCCY 1 cut(s) 1360
EcoO65I GGTNACC 1 cut(s) 2510
EcoRI GAATTC 3 cut(s) 3412, 5458, 6178
EcoRII CCWGG 4 cut(s) 1197, 1945, 4050, 6554
EcoRV GATATC 3 cut(s) 1449, 3045, 5628
EcoT14I CCWWGG 8 cut(s) 442, 971, 1852, 2583, 4673, 5010, 5517, 6578
EcoT22I ATGCAT 6 cut(s) 211, 3279, 3974, 4101, 4911, 4983
EcoT38I GRGCYC 3 cut(s) 869, 2169, 3272
ErhI CCWWGG 8 cut(s) 442, 971, 1852, 2583, 4673, 5010, 5517, 6578
Esp3I CGTCTC 2 cut(s) 162, 5648
FaqI GGGAC 3 cut(s) 1432, 3729, 4029
FauI CCCGC 1 cut(s) 140
FauNDI CATATG 1 cut(s) 4905
FbaI TGATCA 4 cut(s) 351, 2014, 2737, 4900
FblI GTMKAC 5 cut(s) 3114, 3284, 3328, 5191, 5955
FriOI GRGCYC 3 cut(s) 869, 2169, 3272
FspAI RTGCGCAY 1 cut(s) 4616
FspI TGCGCA 1 cut(s) 4616
GlaI GCGC 6 cut(s) 1707, 2243, 3632, 4616, 4816, 4970
GsaI CCCAGC 2 cut(s) 281, 3707
GsuI CTGGAG 5 cut(s) 614, 2377, 5380, 6006, 6577
HaeII RGCGCY 1 cut(s) 1709
HaeIII GGCC 7 cut(s) 27, 1361, 1597, 1930, 4345, 5994, 6342
HapII CCGG 1 cut(s) 1163
HgaI GACGC 2 cut(s) 4806, 4943
HhaI GCGC 6 cut(s) 1708, 2244, 3633, 4617, 4817, 4971
Hin6I GCGC 6 cut(s) 1706, 2242, 3631, 4615, 4815, 4969
HinP1I GCGC 6 cut(s) 1706, 2242, 3631, 4615, 4815, 4969
HincII GTYRAC 1 cut(s) 4933
HindII GTYRAC 1 cut(s) 4933
HindIII AAGCTT 6 cut(s) 92, 514, 1219, 3050, 5162, 6233
HpaII CCGG 1 cut(s) 1163
HphI GGTGA 9 cut(s) 204, 1041, 1344, 1562, 1760, 2350, 4359, 5055, 6059
Hpy99I CGWCG 1 cut(s) 5924
HpyCH4IV ACGT 9 cut(s) 701, 1458, 2118, 2526, 2694, 3891, 4437, 6255, 6529
HpySE526I ACGT 9 cut(s) 701, 1458, 2118, 2526, 2694, 3891, 4437, 6255, 6529
HspAI GCGC 6 cut(s) 1706, 2242, 3631, 4615, 4815, 4969
Ksp22I TGATCA 4 cut(s) 351, 2014, 2737, 4900
LguI GCTCTTC 1 cut(s) 4164
LmnI GCTCC 7 cut(s) 350, 525, 767, 894, 3724, 4642, 4925
MaeII ACGT 9 cut(s) 701, 1458, 2118, 2526, 2694, 3891, 4437, 6255, 6529
MbiI CCGCTC 1 cut(s) 345
MfeI CAATTG 3 cut(s) 2758, 3864, 4620
MhlI GDGCHC 7 cut(s) 819, 869, 932, 2169, 3272, 3297, 6019
MlyI GAGTC 7 cut(s) 538, 653, 988, 2201, 5467, 5522, 6095
MmeI TCCRAC 6 cut(s) 1699, 2332, 2825, 4205, 4428, 6576
Mph1103I ATGCAT 6 cut(s) 211, 3279, 3974, 4101, 4911, 4983
MroXI GAANNNNTTC 7 cut(s) 1183, 1277, 1280, 2687, 3579, 5215, 5526
MslI CAYNNNNRTG 8 cut(s) 217, 3953, 4329, 4908, 6008, 6123, 6495, 6636
MspCI CTTAAG 1 cut(s) 95
MspI CCGG 1 cut(s) 1163
MspR9I CCNGG 5 cut(s) 1164, 1199, 1947, 4052, 6556
MunI CAATTG 3 cut(s) 2758, 3864, 4620
Mva1269I GAATGC 3 cut(s) 3655, 4356, 4415
MvaI CCWGG 4 cut(s) 1199, 1947, 4052, 6556
MvnI CGCG 2 cut(s) 4240, 4817
NciI CCSGG 1 cut(s) 1164
NcoI CCATGG 1 cut(s) 6578
NdeI CATATG 1 cut(s) 4905
NlaIV GGNNCC 8 cut(s) 275, 1362, 2109, 3881, 4333, 6261, 6341, 6588
NmuCI GTSAC 7 cut(s) 1058, 1350, 1550, 3343, 5149, 5803, 6251
NsbI TGCGCA 1 cut(s) 4616
NsiI ATGCAT 6 cut(s) 211, 3279, 3974, 4101, 4911, 4983
NspI RCATGY 7 cut(s) 473, 2798, 4649, 4913, 5133, 5447, 6330
NspV TTCGAA 1 cut(s) 511
PaeI GCATGC 1 cut(s) 2798
PaeR7I CTCGAG 1 cut(s) 5750
PagI TCATGA 3 cut(s) 312, 2413, 3538
PceI AGGCCT 1 cut(s) 4345
PciI ACATGT 3 cut(s) 469, 5129, 5443
PciSI GCTCTTC 1 cut(s) 4164
PctI GAATGC 3 cut(s) 3655, 4356, 4415
PdmI GAANNNNTTC 7 cut(s) 1183, 1277, 1280, 2687, 3579, 5215, 5526
PflMI CCANNNNNTGG 3 cut(s) 23, 4849, 6619
PleI GAGTC 7 cut(s) 538, 653, 988, 2201, 5467, 5521, 6095
PpsI GAGTC 7 cut(s) 538, 653, 988, 2201, 5467, 5521, 6095
Ppu21I YACGTR 1 cut(s) 1459
PscI ACATGT 3 cut(s) 469, 5129, 5443
PshBI ATTAAT 2 cut(s) 3155, 3594
Psp124BI GAGCTC 1 cut(s) 3272
Psp6I CCWGG 4 cut(s) 1197, 1945, 4050, 6554
PspEI GGTNACC 1 cut(s) 2510
PspFI CCCAGC 2 cut(s) 277, 3703
PspGI CCWGG 4 cut(s) 1197, 1945, 4050, 6554
PspN4I GGNNCC 8 cut(s) 275, 1362, 2109, 3881, 4333, 6261, 6341, 6588
PspPI GGNCC 4 cut(s) 692, 1360, 2150, 6340
PspXI VCTCGAGB 1 cut(s) 5750
PstI CTGCAG 5 cut(s) 927, 1345, 1386, 4719, 5242
PstNI CAGNNNCTG 4 cut(s) 2210, 3710, 4895, 6104
RseI CAYNNNNRTG 8 cut(s) 217, 3953, 4329, 4908, 6008, 6123, 6495, 6636
SacI GAGCTC 1 cut(s) 3272
SapI GCTCTTC 1 cut(s) 4164
Sau96I GGNCC 4 cut(s) 692, 1360, 2150, 6340
ScaI AGTACT 2 cut(s) 1108, 2612
SchI GAGTC 7 cut(s) 538, 653, 988, 2201, 5467, 5522, 6095
ScrFI CCNGG 5 cut(s) 1164, 1199, 1947, 4052, 6556
SduI GDGCHC 7 cut(s) 819, 869, 932, 2169, 3272, 3297, 6019
Sfr274I CTCGAG 1 cut(s) 5750
SfuI TTCGAA 1 cut(s) 511
SinI GGWCC 2 cut(s) 692, 2150
SlaI CTCGAG 1 cut(s) 5750
SmiMI CAYNNNNRTG 8 cut(s) 217, 3953, 4329, 4908, 6008, 6123, 6495, 6636
SmlI CTYRAG 8 cut(s) 95, 422, 485, 571, 782, 1709, 5468, 5750
SmoI CTYRAG 8 cut(s) 95, 422, 485, 571, 782, 1709, 5468, 5750
SpeI ACTAGT 1 cut(s) 3320
SphI GCATGC 1 cut(s) 2798
SseBI AGGCCT 1 cut(s) 4345
SsiI CCGC 9 cut(s) 70, 147, 343, 587, 678, 4468, 5404, 5945, 6441
SspI AATATT 3 cut(s) 1812, 3007, 3463
SstI GAGCTC 1 cut(s) 3272
StuI AGGCCT 1 cut(s) 4345
StyD4I CCNGG 5 cut(s) 1162, 1197, 1945, 4050, 6554
StyI CCWWGG 8 cut(s) 442, 971, 1852, 2583, 4673, 5010, 5517, 6578
TaiI ACGT 9 cut(s) 704, 1461, 2121, 2529, 2697, 3894, 4440, 6258, 6532
TaqII GACCGA 2 cut(s) 119, 3690
TatI WGTACW 6 cut(s) 1008, 1106, 1793, 2282, 2610, 4117
TauI GCSGC 2 cut(s) 73, 6443
TseFI GTSAC 7 cut(s) 1058, 1350, 1550, 3343, 5149, 5803, 6251
Tsp45I GTSAC 7 cut(s) 1058, 1350, 1550, 3343, 5149, 5803, 6251
TspGWI ACGGA 2 cut(s) 4723, 5592
Van91I CCANNNNNTGG 3 cut(s) 23, 4849, 6619
Vha464I CTTAAG 1 cut(s) 95
VpaK11BI GGWCC 2 cut(s) 692, 2150
VspI ATTAAT 2 cut(s) 3155, 3594
XagI CCTNNNNNAGG 1 cut(s) 4339
XbaI TCTAGA 2 cut(s) 4088, 4417
XceI RCATGY 7 cut(s) 473, 2798, 4649, 4913, 5133, 5447, 6330
XhoI CTCGAG 1 cut(s) 5750
XmiI GTMKAC 5 cut(s) 3114, 3284, 3328, 5191, 5955
XmnI GAANNNNTTC 7 cut(s) 1183, 1277, 1280, 2687, 3579, 5215, 5526
ZrmI AGTACT 2 cut(s) 1108, 2612
Zsp2I ATGCAT 6 cut(s) 211, 3279, 3974, 4101, 4911, 4983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.