Rorug05G0177300

Nucleolar pre-ribosomal-associated protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002923
Physical Location & Seq
Forward (+)
16577210 .. 16580859
3650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0177300.1

Sequence Viewer

Length: 1446 bp
ATGGCCTCTGCCTCCGCACCCACAACTTTCTCCCTCCTCCAATCCACAACCGCCTCCTCCACCTCCCGCGCCGCCCGCTCCTCCCCCTCCCATGTCTCCCTCCCCTCCTCCCTCAAACCCACCATCCGCCGCCTGGGTTTCGCCGCCGCCGACCCCCTCTTCGCCTTCCAAGTCGCCGCCAAAGTCAGGTCCTTTGGCTCCGGCAAGGCCGTCAGGGGCGTCGTGGCCATGGCCAAGAAGAGCGTTGGTGACTTGACCGAGGCTGATTTGAAGGGGAAGAAGGTGTTTGTCAGAGCTGACTTGAATGTGCCTTTGGATGATAGCCAGAACATTACTGATGATACTAGAATAAGGGCGGCTATTCCTACTATTAAGTATTTGATTCAGAAGGGGGCTAAAGTCATTCTCTCAAGCCATTTGGGACGACCAAAAGGTGTGACCCCAAAGTTTAGCTTGGCACCTCTTGTACCTCGGCTATCTGAACTTCTTGGCATTCAGGTCGTGAAGGCTGAAGACTCTATTGGTCCAGAAGTAGAAAAGCTGGTGGCTTCACTTCCTGATGGTGGTGTCCTTCTTCTTGAAAATGTGAGGTTTTACAAAGAGGAGGAGAAGAACGATCCTGAGCATGCAAAGAAGCTCGCCTCTGTAGCTGATCTTTTTGTCAATGATGCATTCGGAACTGCACATAGAGCCCATGCTTCAACTGAGGGTGTAACAAAGTTCTTAAGGCCATCTGTAGCTGGTTTTCTTTTGCAGAAGGAACTCGACTATCTTGTTGGAGCAGTATCAAACCCAAAAAAGCCATTTGCAGCCATTGTTGGTGGTTCGAAGGTCTCATCCAAGATTGGAGTGATCGAGTCGCTGTTAGAGAAGGTTGATTACTTAATTCTTGGTGGAGGAATGATCTTCACATTTTACAAGGCACAGGGTATCTCAGTGGGTTCATCTCTGGTGGAAGAGGATAAGCTAGAACTTGCTACATCACTCATTGCAAAGGCCAAGGCGAAGGGAGTGTCTCTTTTGCTACCCACTGATGTCGTAATTGCAGACAAATTTGCTCCTGATGCAAACAGCAAGATTGTTCCAGCTTCAAGCATCCCTGACGGGTGGATGGGATTGGATATTGGACCAGACTCTATTAAGACATTCAATGATGCACTTGATACCACTCAAACCATCATTTGGAACGGACCAATGGGAGTGTTTGAGTTTGACAAGTTTGCAGTAGGAACAGAGGCTATTGCAAAGAAGCTTGCAGAGCTTAGTGGCAAGGGAGTGACAACCATCATCGGAGGTGGAGACTCAGTTGCGGCTGTGGAGAAAGTAGGAGTTGCTAGTGTGATGAGCCACATATCAACTGGCGGTGGTGCTAGTTTGGAGTTGTTGGAAGGCAAAGAACTTCCTGGTGTACTTGCTCTTGATGAAGCCATTCCAGTTCCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NopRA1 Npa1

Protein Analysis

481

Amino Acids

50.31

Weight (kDa)

8.38

Isoelectric Point (pI)

32.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGK PF00162 86 - 465 1.4e-163 Phosphoglycerate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 457
AccB7I CCANNNNNTGG 1 cut(s) 1182
AccBSI CCGCTC 1 cut(s) 78
AccII CGCG 1 cut(s) 69
AclWI GGATC 1 cut(s) 611
AcoI YGGCCR 2 cut(s) 225, 231
AcsI RAATTY 1 cut(s) 1052
AcuI CTGAAG 1 cut(s) 531
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 2 cut(s) 468, 1410
AfiI CCNNNNNNNGG 4 cut(s) 133, 186, 563, 1182
AflII CTTAAG 1 cut(s) 724
AgsI TTSAA 6 cut(s) 271, 304, 581, 702, 1092, 1150
AjnI CCWGG 2 cut(s) 132, 1402
AjuI GAANNNNNNNTTGG 4 cut(s) 296, 328, 504, 536
Alw26I GTCTC 4 cut(s) 100, 838, 1020, 1293
AlwI GGATC 1 cut(s) 611
AlwNI CAGNNNCTG 1 cut(s) 1439
AoxI GGCC 6 cut(s) 3, 207, 225, 231, 728, 996
ApeKI GCWGC 1 cut(s) 809
ApoI RAATTY 1 cut(s) 1052
ArsI GACNNNNNNTTYG 4 cut(s) 143, 175, 998, 1030
Asp700I GAANNNNTTC 1 cut(s) 1428
AspLEI GCGC 1 cut(s) 71
AspS9I GGNCC 4 cut(s) 189, 524, 1127, 1190
AsuHPI GGTGA 1 cut(s) 260
AsuII TTCGAA 1 cut(s) 827
AvaII GGWCC 4 cut(s) 189, 524, 1127, 1190
BalI TGGCCA 2 cut(s) 227, 233
BanI GGYRCC 1 cut(s) 457
BanII GRGCYC 1 cut(s) 694
BarI GAAGNNNNNNTAC 2 cut(s) 863, 895
BbsI GAAGAC 1 cut(s) 519
BbvI GCAGC 1 cut(s) 821
BccI CCATC 6 cut(s) 131, 554, 739, 1105, 1184, 1292
BceAI ACGGC 1 cut(s) 194
BcgI CGANNNNNNTGC 2 cut(s) 481, 515
BciT130I CCWGG 2 cut(s) 134, 1404
BcoDI GTCTC 4 cut(s) 100, 838, 1020, 1293
BfaI CTAG 4 cut(s) 345, 968, 1335, 1371
BfmI CTRYAG 2 cut(s) 645, 735
BfrI CTTAAG 1 cut(s) 724
BisI GCNGC 8 cut(s) 72, 130, 144, 147, 177, 357, 810, 1311
BlsI GCNGC 8 cut(s) 73, 131, 145, 148, 178, 358, 811, 1312
Bme1390I CCNGG 2 cut(s) 134, 1404
Bme18I GGWCC 4 cut(s) 189, 524, 1127, 1190
BmgT120I GGNCC 4 cut(s) 189, 524, 1127, 1190
BmiI GGNNCC 2 cut(s) 199, 459
BmrFI CCNGG 2 cut(s) 134, 1404
BmsI GCATC 4 cut(s) 658, 1054, 1104, 1144
BpiI GAAGAC 1 cut(s) 519
Bpu10I CCTNAGC 1 cut(s) 621
Bpu14I TTCGAA 1 cut(s) 827
BpuEI CTTGAG 1 cut(s) 394
BsaHI GRCGYC 1 cut(s) 219
BsaI GGTCTC 1 cut(s) 838
BsaJI CCNNGG 5 cut(s) 133, 228, 258, 470, 999
BsaXI ACNNNNNCTCC 2 cut(s) 1290, 1320
Bsc4I CCNNNNNNNGG 4 cut(s) 133, 186, 563, 1182
Bse1I ACTGG 2 cut(s) 1363, 1433
Bse3DI GCAATG 1 cut(s) 987
BseBI CCWGG 2 cut(s) 134, 1404
BseDI CCNNGG 5 cut(s) 133, 228, 258, 470, 999
BseGI GGATG 5 cut(s) 123, 322, 836, 1095, 1116
BseLI CCNNNNNNNGG 4 cut(s) 133, 186, 563, 1182
BseMI GCAATG 1 cut(s) 987
BseMII CTCAG 4 cut(s) 612, 696, 948, 1317
BseNI ACTGG 2 cut(s) 1363, 1433
BseRI GAGGAG 6 cut(s) 26, 46, 70, 97, 617, 620
BseXI GCAGC 1 cut(s) 821
BsgI GTGCAG 1 cut(s) 666
Bsh1236I CGCG 1 cut(s) 69
BshFI GGCC 6 cut(s) 5, 209, 227, 233, 730, 998
BshNI GGYRCC 1 cut(s) 457
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 435
BslI CCNNNNNNNGG 4 cut(s) 133, 186, 563, 1182
BsmAI GTCTC 4 cut(s) 100, 838, 1020, 1293
BsmFI GGGAC 1 cut(s) 435
BsmI GAATGC 2 cut(s) 492, 671
BsnI GGCC 6 cut(s) 5, 209, 227, 233, 730, 998
Bso31I GGTCTC 1 cut(s) 838
Bsp119I TTCGAA 1 cut(s) 827
Bsp1286I GDGCHC 1 cut(s) 694
Bsp143I GATC 4 cut(s) 616, 652, 852, 903
Bsp19I CCATGG 1 cut(s) 228
BspANI GGCC 6 cut(s) 5, 209, 227, 233, 730, 998
BspCNI CTCAG 4 cut(s) 613, 697, 947, 1316
BspFNI CGCG 1 cut(s) 69
BspLI GGNNCC 2 cut(s) 199, 459
BspPI GGATC 1 cut(s) 611
BspQI GCTCTTC 1 cut(s) 233
BspT104I TTCGAA 1 cut(s) 827
BspT107I GGYRCC 1 cut(s) 457
BspTI CTTAAG 1 cut(s) 724
BspTNI GGTCTC 1 cut(s) 838
BsrBI CCGCTC 1 cut(s) 78
BsrDI GCAATG 1 cut(s) 987
BsrI ACTGG 2 cut(s) 1363, 1433
BssECI CCNNGG 5 cut(s) 133, 228, 258, 470, 999
BssMI GATC 4 cut(s) 616, 652, 852, 903
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 2 cut(s) 228, 999
Bst2UI CCWGG 2 cut(s) 134, 1404
Bst6I CTCTTC 3 cut(s) 164, 233, 951
BstACI GRCGYC 1 cut(s) 219
BstAFI CTTAAG 1 cut(s) 724
BstBI TTCGAA 1 cut(s) 827
BstC8I GCNNGC 4 cut(s) 76, 627, 639, 1254
BstDEI CTNAG 5 cut(s) 621, 705, 934, 1262, 1303
BstDSI CCRYGG 1 cut(s) 228
BstF5I GGATG 5 cut(s) 123, 322, 836, 1095, 1116
BstFNI CGCG 1 cut(s) 69
BstHHI GCGC 1 cut(s) 71
BstKTI GATC 4 cut(s) 619, 655, 855, 906
BstMAI GTCTC 4 cut(s) 100, 838, 1020, 1293
BstMBI GATC 4 cut(s) 616, 652, 852, 903
BstMWI GCNNNNNNNGC 5 cut(s) 75, 647, 689, 1064, 1258
BstNI CCWGG 2 cut(s) 134, 1404
BstNSI RCATGY 1 cut(s) 629
BstSCI CCNGG 2 cut(s) 132, 1402
BstSFI CTRYAG 2 cut(s) 645, 735
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 1 cut(s) 821
BstV2I GAAGAC 1 cut(s) 519
BsuRI GGCC 6 cut(s) 5, 209, 227, 233, 730, 998
BtgI CCRYGG 1 cut(s) 228
BtsCI GGATG 5 cut(s) 123, 322, 836, 1095, 1116
BtsIMutI CAGTG 2 cut(s) 942, 1029
Cac8I GCNNGC 4 cut(s) 76, 627, 639, 1254
CaiI CAGNNNCTG 1 cut(s) 1439
CfoI GCGC 1 cut(s) 71
Cfr13I GGNCC 4 cut(s) 189, 524, 1127, 1190
CseI GACGC 1 cut(s) 208
Csp6I GTAC 2 cut(s) 467, 1409
CviAII CATG 4 cut(s) 92, 229, 626, 695
CviQI GTAC 2 cut(s) 467, 1409
DdeI CTNAG 5 cut(s) 621, 705, 934, 1262, 1303
DpnI GATC 4 cut(s) 618, 654, 854, 905
DpnII GATC 4 cut(s) 616, 652, 852, 903
EaeI YGGCCR 2 cut(s) 225, 231
Eam1104I CTCTTC 3 cut(s) 164, 233, 951
EarI CTCTTC 3 cut(s) 164, 233, 951
EciI GGCGGA 1 cut(s) 116
Eco130I CCWWGG 2 cut(s) 228, 999
Eco24I GRGCYC 1 cut(s) 694
Eco31I GGTCTC 1 cut(s) 838
Eco47I GGWCC 4 cut(s) 189, 524, 1127, 1190
Eco57I CTGAAG 1 cut(s) 531
EcoO109I RGGNCCY 1 cut(s) 189
EcoRII CCWGG 2 cut(s) 132, 1402
EcoT14I CCWWGG 2 cut(s) 228, 999
EcoT22I ATGCAT 1 cut(s) 673
EcoT38I GRGCYC 1 cut(s) 694
ErhI CCWWGG 2 cut(s) 228, 999
FaeI CATG 4 cut(s) 95, 232, 629, 698
FaiI YATR 6 cut(s) 93, 230, 627, 687, 696, 1352
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FaqI GGGAC 1 cut(s) 435
FatI CATG 4 cut(s) 91, 228, 625, 694
FauI CCCGC 2 cut(s) 74, 83
Fnu4HI GCNGC 8 cut(s) 72, 130, 144, 147, 177, 357, 810, 1311
FokI GGATG 5 cut(s) 110, 329, 823, 1082, 1123
FriOI GRGCYC 1 cut(s) 694
Fsp4HI GCNGC 8 cut(s) 72, 130, 144, 147, 177, 357, 810, 1311
FspBI CTAG 4 cut(s) 345, 968, 1335, 1371
GlaI GCGC 1 cut(s) 70
GluI GCNGC 8 cut(s) 72, 130, 144, 147, 177, 357, 810, 1311
HaeIII GGCC 6 cut(s) 5, 209, 227, 233, 730, 998
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 208
HhaI GCGC 1 cut(s) 71
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 4 cut(s) 95, 232, 629, 698
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HindIII AAGCTT 1 cut(s) 1250
HinfI GANTC 5 cut(s) 382, 515, 857, 1133, 1301
HpaII CCGG 1 cut(s) 201
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 1 cut(s) 1409
Hpy188I TCNGA 5 cut(s) 293, 387, 481, 677, 1292
Hpy188III TCNNGA 7 cut(s) 502, 527, 557, 578, 620, 1061, 1418
Hpy8I GTNNAC 1 cut(s) 1409
Hpy99I CGWCG 1 cut(s) 224
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 647, 689, 1064, 1258
HpyF3I CTNAG 5 cut(s) 621, 705, 934, 1262, 1303
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 4 cut(s) 95, 232, 629, 698
HspAI GCGC 1 cut(s) 69
Kzo9I GATC 4 cut(s) 616, 652, 852, 903
LguI GCTCTTC 1 cut(s) 233
LmnI GCTCC 4 cut(s) 83, 203, 779, 1063
Lsp1109I GCAGC 1 cut(s) 821
LweI GCATC 4 cut(s) 658, 1054, 1104, 1144
MaeI CTAG 4 cut(s) 345, 968, 1335, 1371
MaeIII GTNAC 4 cut(s) 248, 436, 712, 1276
MalI GATC 4 cut(s) 618, 654, 854, 905
MbiI CCGCTC 1 cut(s) 78
MboI GATC 4 cut(s) 616, 652, 852, 903
MboII GAAGA 8 cut(s) 151, 250, 289, 524, 566, 622, 898, 968
MhlI GDGCHC 1 cut(s) 694
MlsI TGGCCA 2 cut(s) 227, 233
MluCI AATT 3 cut(s) 885, 1041, 1052
MluNI TGGCCA 2 cut(s) 227, 233
MlyI GAGTC 4 cut(s) 509, 866, 1127, 1295
MmeI TCCRAC 2 cut(s) 757, 1365
Mox20I TGGCCA 2 cut(s) 227, 233
Mph1103I ATGCAT 1 cut(s) 673
MroXI GAANNNNTTC 1 cut(s) 1428
MscI TGGCCA 2 cut(s) 227, 233
MseI TTAA 4 cut(s) 372, 725, 884, 1140
Msp20I TGGCCA 2 cut(s) 227, 233
MspCI CTTAAG 1 cut(s) 724
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 2 cut(s) 134, 1404
Mva1269I GAATGC 2 cut(s) 492, 671
MvaI CCWGG 2 cut(s) 134, 1404
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 5 cut(s) 75, 647, 689, 1064, 1258
NcoI CCATGG 1 cut(s) 228
NdeII GATC 4 cut(s) 616, 652, 852, 903
NlaIII CATG 4 cut(s) 95, 232, 629, 698
NlaIV GGNNCC 2 cut(s) 199, 459
NmeAIII GCCGAG 1 cut(s) 451
NmuCI GTSAC 3 cut(s) 248, 436, 1276
NsiI ATGCAT 1 cut(s) 673
NspI RCATGY 1 cut(s) 629
NspV TTCGAA 1 cut(s) 827
PaeI GCATGC 1 cut(s) 629
PciSI GCTCTTC 1 cut(s) 233
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PctI GAATGC 2 cut(s) 492, 671
PdmI GAANNNNTTC 1 cut(s) 1428
PfeI GAWTC 1 cut(s) 382
PflMI CCANNNNNTGG 1 cut(s) 1182
PkrI GCNGC 8 cut(s) 73, 131, 145, 148, 178, 358, 811, 1312
PleI GAGTC 4 cut(s) 509, 865, 1127, 1295
PpsI GAGTC 4 cut(s) 509, 865, 1127, 1295
PpuMI RGGWCCY 1 cut(s) 189
Psp5II RGGWCCY 1 cut(s) 189
Psp6I CCWGG 2 cut(s) 132, 1402
PspGI CCWGG 2 cut(s) 132, 1402
PspN4I GGNNCC 2 cut(s) 199, 459
PspPI GGNCC 4 cut(s) 189, 524, 1127, 1190
PspPPI RGGWCCY 1 cut(s) 189
PstNI CAGNNNCTG 1 cut(s) 1439
RsaI GTAC 2 cut(s) 468, 1410
RsaNI GTAC 2 cut(s) 467, 1409
SapI GCTCTTC 1 cut(s) 233
SaqAI TTAA 4 cut(s) 372, 725, 884, 1140
SatI GCNGC 8 cut(s) 72, 130, 144, 147, 177, 357, 810, 1311
Sau3AI GATC 4 cut(s) 616, 652, 852, 903
Sau96I GGNCC 4 cut(s) 189, 524, 1127, 1190
SchI GAGTC 4 cut(s) 509, 866, 1127, 1295
ScrFI CCNGG 2 cut(s) 134, 1404
SduI GDGCHC 1 cut(s) 694
SfaNI GCATC 4 cut(s) 658, 1054, 1104, 1144
SfcI CTRYAG 2 cut(s) 645, 735
SfuI TTCGAA 1 cut(s) 827
SinI GGWCC 4 cut(s) 189, 524, 1127, 1190
SmlI CTYRAG 2 cut(s) 409, 724
SmoI CTYRAG 2 cut(s) 409, 724
SphI GCATGC 1 cut(s) 629
Sse9I AATT 3 cut(s) 885, 1041, 1052
SspMI CTAG 4 cut(s) 345, 968, 1335, 1371
StyD4I CCNGG 2 cut(s) 132, 1402
StyI CCWWGG 2 cut(s) 228, 999
TaqI TCGA 3 cut(s) 765, 827, 855
TaqII GACCGA 1 cut(s) 272
TasI AATT 3 cut(s) 885, 1041, 1052
TatI WGTACW 1 cut(s) 1408
TauI GCSGC 7 cut(s) 74, 132, 146, 149, 179, 359, 1313
TfiI GAWTC 1 cut(s) 382
Tru1I TTAA 4 cut(s) 372, 725, 884, 1140
Tru9I TTAA 4 cut(s) 372, 725, 884, 1140
TscAI CASTG 2 cut(s) 942, 1036
TseFI GTSAC 3 cut(s) 248, 436, 1276
TseI GCWGC 1 cut(s) 809
Tsp45I GTSAC 3 cut(s) 248, 436, 1276
TspDTI ATGAA 2 cut(s) 933, 1437
TspGWI ACGGA 1 cut(s) 1203
TspRI CASTG 2 cut(s) 942, 1036
Van91I CCANNNNNTGG 1 cut(s) 1182
Vha464I CTTAAG 1 cut(s) 724
VpaK11BI GGWCC 4 cut(s) 189, 524, 1127, 1190
XapI RAATTY 1 cut(s) 1052
XceI RCATGY 1 cut(s) 629
XcmI CCANNNNNNNNNTGG 2 cut(s) 130, 1355
XmnI GAANNNNTTC 1 cut(s) 1428
XspI CTAG 4 cut(s) 345, 968, 1335, 1371
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.