pycom03g18780

presynapse organization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005139
Physical Location & Seq
Forward (+)
19649455 .. 19654057
4603 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3195 bp
ATGTTTCTCAGCAAAGAAATTGACGATTTGCATGATGATGGGTTTGAAGGATCGAAAAATGAGCACTGTATATTCACAGAGGCTTTCTTTGGTCAAGACATTGGTCGTACTAGTAATAGATGTCTTGTTACTGGAATGATTAACTTTGAATGTGAGTCAAGTAAGAACACTGATGGAGCACCGTCTCCAAACAGTGAAAACTCAGTTGTAACGAGTCACTCCTCAAAAAATATCTGTTTAGAGGAGTTTTATAAAGCAGCCGAAGATTTTAGAGAAACCTCTGCACCAGGGTTTTCCTTGGACAGATCTGCTTTGTTAGATAGGAATGGTGATGATGCGACTGTTAATAGAATGACGTTTTCAGTTGATGAGCTTCCTAATACCAAACCTGGTTTAGGAAGGGTCACATCTGGTCTAGCTTCAAACTCTGTCTCTGAAACAGTGACATTTCGTTTGGTTGAATCTTCTAGCCAGGGAGTCACGTCTAGTTGTTATCTGTTTAAGCAACATGCAGAACTTAACAAACTGGGCGTAGCAGGTGACCCAGATGTTTCAAAGTGCAGATTGCGAAACTCAGATGGGGACGATAGAAAGGAAGTCTGCCTAAGTAAAGCTATTGCTTCACATGAGAACTTCTTGACCAGGCTTCTGGTTGCAAGTCCATTAGCCACTGTTATGGATAAATCCAGAACTCCTCTACATTCTGAGGGAAAGCCCAACGGATTTGAATCTTCTGTTTTGGATGTCTCCAATGTTGCCTTGAAGATGGAGAATAGTAGGGACCCTCGTCCGCTTCTCCGATATCATGTTGTTCGCTTGCTTGAAGCTGCTGGATGGCATATTGAGAGGCGAAAAAGACCTAGTAGAAATTATATGGAGACTGTTTATATAACACCTAAGGGAAGGTTAATTTGGGAACTCCCCAAAGCTTGGCGGTTATGTGGAGAAATTCTGTTAGCAGATGGGTACAGTATGCTGCAGGAAGATACAAAGGAGTGGGCCAATATCAGTCAGTTCTGGTCAGATCTGTCTGGTGTTTTAGTAAATATTGAGAAAGAGATTCATCATCCACAACCTGCTACTGCATTGGCTTACTGGTGGAGGCTTTTAGATCCTTTTGTGGTTGTCGTGTTTATTGAGAGAAAGATTGGTAGTCTAAGAAAGGGAGAGATAGTTAAAGCAACTGAAAGTATAGCTGATTCGAACCGTAAGATTGATTCTGCTTTGGCCTTGACTAGTGCCAATAACATAGAAAGTCATTTTACTAAGGAAGATGTGTCAGCTCCTCTCTGTGATTCCACACTGGCTAGGAGGGTTGGATTGGCAGTTTCTAAGCGAGATTACAATGCCTGTGGGAAATCTGGTAATGAAAGTTCCTCAAAACATTGCGGACAGACAAGTAATGAGGAAGTGAAATTTCTTATGGGACTATCTATTTATTCAGCTATTGCAGAAAGTCATTGCTTGGTGAATGCTGGTAATAGAGTTGAAAATGGGTTCTCTGGGAAAATAACTTGTCCGGATTCCTTACCAGTTTGTGGGTCTGGTGGCATTTGTATTCAGTCAGCTACTCATCGAGATGATCCCATTACTTCTAGAAATTGTAACAATGTGCATGGAGGTTCTGAAGCTGTCTCGCCTCACCAAGATGGCAATGCAAATTCCCCTGGCTCTAATAAACAGAGTTCTGGACTTGATTTAGAAACGCCTAAGGAAGTTATGGAAGACACCTCAGTGGATTCTTCTGAGGAAAAAGATGAATTGGAGGGAGGCAAGGTTGATGATAAGCTGGAAAGTGCTCAGAAGGGATCATTAGATAACCAAGGAAACTACACAATTGATGTTCTGAAAAGAAAAACACGTAGGAAGTCTAAAAAGATATCTGAAATTGAACCAAGCTCGTTATACCGAAGTGGCCTTTTAGATTTTACTTCAAGTGAGAATGCTGATTCCCTGTGTATTGATGCTAATGGTACCCAGTCAAAGTCGACAGAGGTCCAAGATGAATTTACAGGCAATAAAAGATGCAAAGGAAGTCGCAAGATGTACTTACCTACCGACTCTTGCCAGCAACAGATTGGGAGAAAAAGTTCAAAGTTGATGAAGATTTCACGTGAATGTGATGATGTCCAGACTGGAAGAAGTAAATCATTTAAATGCCAAATTGAAGATGATGACTTGTTGGTTTCAGCTATTATAAAAAATAAAGATTTTAGGACAACGCATGCTCAATATTCTTCTAGAAAGAAAGCCTACAAATCAAGAGCTCATAAGAAAGGTAAAACTAAAAGCCAAAAGAGTCATTGCAAGTTGCTTCCAAGAAGCCTGGGCAGTGGGGGAAAGCACTTTAAGGATGGAAAATGGCATTCTGTAGGAGTGAAAACAGTTTTGTCTTGGTTAATTGATGCTGGTGTCTTATCTCCAGATGATGTGATTCAGTATCGGAATCCTAAGGATGGTGGTGTCCTTATAGATGGTCTGGTTACTAGGGATGGCATTTTTTGCAAGTGTTGCAGTAAAGTAGTTTCAGTTTCTGAGTTCAAGAGTCATGGTGGGTTCAAACAGAATCGTCCATGTTTGAATCTTTTCATGGAATCTGGTCAGTCCTTCACCTTATGCCAGCTTCAGGCTTGGTCAGCTGAATACAAGACCAGGAAAAAGAGCACCCACGTCGTGCGAGATGATGCAAATGATCAAAATGATGATTCATGTGGACTTTGTGGTGATGGTGGCGAGCTGATATGCTGTGATAATTGCCCCTCTACTTTTCATCAGGCTTGTTTATCTCTGCAGGAGCTCCCTGAAGGCAACTGGTATTGCTCGAATTGCACCTGTTGGATATGTGGTGATTTCGTCAATGATAAGGGGGCTTCGAGTCCTGATGGATTTAAGTGTTCACAGTGTGAGCATAAATATCATGCGGCATGCCTGAAAGATAAATGTACCTACGGAACCATACCGGATACTTATTTCTGTAATAGAAGCTGTCAGGAGGCTTATTCTGGTCTTCAATCTCGCGTTGGCTGCATTAATCATTTTGCTGATGGATTTTCATGGACACTACTTAGATGCATTCATGATGACCAAAAGGGTCATTCTGCTCAACGGTTTGCTCTTAAAGCAGAATGCAATACAAAACTAGCTGTTTCTCTTACAATAATGGAGGAATGCTTTTTGTCAATGGTTGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0004402 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0005911 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0008080 GO:0008134 GO:0008150 GO:0008152 GO:0009506 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010385 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016070 GO:0016407 GO:0016410 GO:0016458 GO:0016569 GO:0016570 GO:0016573 GO:0016581 GO:0016740 GO:0016746 GO:0016747 GO:0017053 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0030054 GO:0031047 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032991 GO:0032993 GO:0034212 GO:0034641 GO:0036211 GO:0042393 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043543 GO:0043966 GO:0043971 GO:0043972 GO:0044030 GO:0044154 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044728 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050808 GO:0051052 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0055044 GO:0060255 GO:0061733 GO:0065007 GO:0070013 GO:0070491 GO:0070603 GO:0071704 GO:0071840 GO:0072553 GO:0080090 GO:0080188 GO:0090304 GO:0090545 GO:0090568 GO:0097159 GO:0099172 GO:1901360 GO:1901363 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1904949 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD

Protein Analysis

1065

Amino Acids

117.51

Weight (kDa)

6.66

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7028 PF22970 244 - 314 4e-06 Domain of unknown function (DUF7028)
TDBD PF16135 826 - 876 1.1e-17 Tify domain binding domain
PHD-1st_NSD PF23011 901 - 943 5.6e-06 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger 1
PHD PF00628 904 - 943 2.6e-07 PHD-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 252, 2198
AarI CACCTGC 1 cut(s) 527
Acc36I ACCTGC 2 cut(s) 527, 1084
Acc65I GGTACC 1 cut(s) 1973
AccB1I GGYRCC 1 cut(s) 1973
AccB7I CCANNNNNTGG 2 cut(s) 930, 1538
AccI GTMKAC 1 cut(s) 1988
AccII CGCG 1 cut(s) 3018
AccIII TCCGGA 1 cut(s) 1519
AciI CCGC 4 cut(s) 791, 934, 1389, 2921
AclWI GGATC 4 cut(s) 58, 1106, 1577, 1816
AcsI RAATTY 4 cut(s) 948, 1415, 1660, 2006
AcuI CTGAAG 3 cut(s) 1647, 2609, 2823
AcvI CACGTG 1 cut(s) 2114
AdeI CACNNNGTG 2 cut(s) 2674, 2903
AfaI GTAC 5 cut(s) 109, 968, 1975, 2048, 2944
AfiI CCNNNNNNNGG 5 cut(s) 395, 930, 1538, 2456, 2626
AflIII ACRYGT 1 cut(s) 1859
AhlI ACTAGT 2 cut(s) 110, 1235
AjiI CACGTC 2 cut(s) 483, 2671
AjnI CCWGG 7 cut(s) 286, 388, 471, 641, 1666, 2325, 2651
AjuI GAANNNNNNNTTGG 2 cut(s) 895, 927
AleI CACNNNNGTG 1 cut(s) 1733
AloI GAACNNNNNNTCC 2 cut(s) 2074, 2106
Alw21I GWGCWC 6 cut(s) 66, 181, 1801, 2269, 2666, 2799
Alw26I GTCTC 5 cut(s) 189, 436, 751, 872, 1639
AlwI GGATC 4 cut(s) 58, 1106, 1577, 1816
AlwNI CAGNNNCTG 1 cut(s) 2534
Aor13HI TCCGGA 1 cut(s) 1519
AoxI GGCC 3 cut(s) 999, 1227, 1915
ApeKI GCWGC 4 cut(s) 257, 827, 976, 3024
ApoI RAATTY 4 cut(s) 948, 1415, 1660, 2006
ArsI GACNNNNNNTTYG 2 cut(s) 436, 468
AseI ATTAAT 1 cut(s) 3030
Asp700I GAANNNNTTC 2 cut(s) 2089, 2585
Asp718I GGTACC 1 cut(s) 1973
AspS9I GGNCC 3 cut(s) 781, 999, 1996
AsuHPI GGTGA 7 cut(s) 341, 551, 1480, 1634, 2602, 2735, 2858
AsuII TTCGAA 1 cut(s) 1202
AvaII GGWCC 2 cut(s) 781, 1996
AxyI CCTNAGG 3 cut(s) 897, 1710, 2451
BanI GGYRCC 1 cut(s) 1973
BanII GRGCYC 2 cut(s) 2269, 2799
BbrPI CACGTG 1 cut(s) 2114
BbsI GAAGAC 2 cut(s) 1731, 2999
Bbv12I GWGCWC 6 cut(s) 66, 181, 1801, 2269, 2666, 2799
BbvI GCAGC 4 cut(s) 269, 814, 963, 3011
BcgI CGANNNNNNTGC 2 cut(s) 2653, 2687
BciT130I CCWGG 7 cut(s) 288, 390, 473, 643, 1668, 2327, 2653
BciVI GTATCC 1 cut(s) 2956
BclI TGATCA 1 cut(s) 2692
BcoDI GTCTC 5 cut(s) 189, 436, 751, 872, 1639
BcuI ACTAGT 2 cut(s) 110, 1235
BfmI CTRYAG 3 cut(s) 977, 2370, 2789
BfuAI ACCTGC 2 cut(s) 527, 1084
BfuI GTATCC 1 cut(s) 2956
BglII AGATCT 2 cut(s) 305, 1024
BisI GCNGC 5 cut(s) 258, 828, 977, 2922, 3025
BlsI GCNGC 5 cut(s) 259, 829, 978, 2923, 3026
Bme1390I CCNGG 7 cut(s) 288, 390, 473, 643, 1668, 2327, 2653
Bme18I GGWCC 2 cut(s) 781, 1996
BmgBI CACGTC 2 cut(s) 483, 2671
BmgT120I GGNCC 3 cut(s) 781, 999, 1996
BmiI GGNNCC 4 cut(s) 782, 783, 1975, 2953
BmrFI CCNGG 7 cut(s) 288, 390, 473, 643, 1668, 2327, 2653
BmrI ACTGGG 2 cut(s) 536, 1972
BmsI GCATC 6 cut(s) 325, 1954, 2015, 2395, 2674, 3059
BmuI ACTGGG 2 cut(s) 536, 1972
BoxI GACNNNNGTC 3 cut(s) 102, 786, 1994
BpiI GAAGAC 2 cut(s) 1731, 2999
BpmI CTGGAG 1 cut(s) 2406
Bpu14I TTCGAA 1 cut(s) 1202
Bsa29I ATCGAT 1 cut(s) 3190
BsaAI YACGTR 2 cut(s) 1862, 2114
BsaBI GATNNNNATC 1 cut(s) 727
BsaJI CCNNGG 6 cut(s) 287, 297, 472, 1666, 1822, 2326
BsaWI WCCGGW 2 cut(s) 1519, 2959
BsaXI ACNNNNNCTCC 6 cut(s) 1158, 1188, 1761, 1791, 2074, 2104
Bsc4I CCNNNNNNNGG 5 cut(s) 395, 930, 1538, 2456, 2626
Bse1I ACTGG 8 cut(s) 136, 531, 1100, 1308, 1532, 1978, 2140, 2816
Bse21I CCTNAGG 3 cut(s) 897, 1710, 2451
Bse3DI GCAATG 4 cut(s) 1384, 1459, 1660, 2302
Bse8I GATNNNNATC 1 cut(s) 727
BseAI TCCGGA 1 cut(s) 1519
BseBI CCWGG 7 cut(s) 288, 390, 473, 643, 1668, 2327, 2653
BseCI ATCGAT 1 cut(s) 3190
BseDI CCNNGG 6 cut(s) 287, 297, 472, 1666, 1822, 2326
BseGI GGATG 6 cut(s) 748, 839, 1066, 2359, 2461, 2497
BseJI GATNNNNATC 1 cut(s) 727
BseLI CCNNNNNNNGG 5 cut(s) 395, 930, 1538, 2456, 2626
BseMI GCAATG 4 cut(s) 1384, 1459, 1660, 2302
BseMII CTCAG 8 cut(s) 22, 216, 588, 696, 1737, 1746, 1814, 2526
BseNI ACTGG 8 cut(s) 136, 531, 1100, 1308, 1532, 1978, 2140, 2816
BseRI GAGGAG 4 cut(s) 211, 257, 684, 1275
BseXI GCAGC 4 cut(s) 269, 814, 963, 3011
BsgI GTGCAG 2 cut(s) 267, 580
Bsh1236I CGCG 1 cut(s) 3018
BshFI GGCC 3 cut(s) 1001, 1229, 1917
BshNI GGYRCC 1 cut(s) 1973
BshVI ATCGAT 1 cut(s) 3190
BsiHKAI GWGCWC 6 cut(s) 66, 181, 1801, 2269, 2666, 2799
BsiSI CCGG 2 cut(s) 1520, 2960
BslFI GGGAC 3 cut(s) 596, 794, 1440
BslI CCNNNNNNNGG 5 cut(s) 395, 930, 1538, 2456, 2626
BsmAI GTCTC 5 cut(s) 189, 436, 751, 872, 1639
BsmBI CGTCTC 1 cut(s) 189
BsmFI GGGAC 3 cut(s) 596, 794, 1440
BsmI GAATGC 6 cut(s) 1477, 1948, 2365, 3072, 3131, 3173
BsnI GGCC 3 cut(s) 1001, 1229, 1917
Bsp119I TTCGAA 1 cut(s) 1202
Bsp1286I GDGCHC 6 cut(s) 66, 181, 1801, 2269, 2666, 2799
Bsp13I TCCGGA 1 cut(s) 1519
Bsp143I GATC 8 cut(s) 50, 305, 1024, 1111, 1582, 1808, 2692, 3187
BspACI CCGC 4 cut(s) 791, 934, 1389, 2921
BspANI GGCC 3 cut(s) 1001, 1229, 1917
BspCNI CTCAG 8 cut(s) 21, 215, 587, 697, 1738, 1745, 1813, 2527
BspDI ATCGAT 1 cut(s) 3190
BspEI TCCGGA 1 cut(s) 1519
BspFNI CGCG 1 cut(s) 3018
BspHI TCATGA 1 cut(s) 3076
BspLI GGNNCC 4 cut(s) 782, 783, 1975, 2953
BspMAI CTGCAG 2 cut(s) 981, 2793
BspMI ACCTGC 2 cut(s) 527, 1084
BspPI GGATC 4 cut(s) 58, 1106, 1577, 1816
BspT104I TTCGAA 1 cut(s) 1202
BspT107I GGYRCC 1 cut(s) 1973
BsrDI GCAATG 4 cut(s) 1384, 1459, 1660, 2302
BsrI ACTGG 8 cut(s) 136, 531, 1100, 1308, 1532, 1978, 2140, 2816
BssECI CCNNGG 6 cut(s) 287, 297, 472, 1666, 1822, 2326
BssMI GATC 8 cut(s) 50, 305, 1024, 1111, 1582, 1808, 2692, 3187
BssT1I CCWWGG 2 cut(s) 297, 1822
Bst2UI CCWGG 7 cut(s) 288, 390, 473, 643, 1668, 2327, 2653
BstAPI GCANNNNNTGC 2 cut(s) 2502, 2511
BstBAI YACGTR 2 cut(s) 1862, 2114
BstBI TTCGAA 1 cut(s) 1202
BstC8I GCNNGC 6 cut(s) 818, 2069, 2226, 2621, 2735, 2926
BstEII GGTNACC 1 cut(s) 539
BstENI CCTNNNNNAGG 1 cut(s) 393
BstF5I GGATG 6 cut(s) 748, 839, 1066, 2359, 2461, 2497
BstFNI CGCG 1 cut(s) 3018
BstKTI GATC 8 cut(s) 53, 308, 1027, 1114, 1585, 1811, 2695, 3190
BstMAI GTCTC 5 cut(s) 189, 436, 751, 872, 1639
BstMBI GATC 8 cut(s) 50, 305, 1024, 1111, 1582, 1808, 2692, 3187
BstMWI GCNNNNNNNGC 6 cut(s) 2502, 2511, 2636, 2826, 3024, 3119
BstNI CCWGG 7 cut(s) 288, 390, 473, 643, 1668, 2327, 2653
BstNSI RCATGY 3 cut(s) 512, 2228, 2928
BstPAI GACNNNNGTC 3 cut(s) 102, 786, 1994
BstPI GGTNACC 1 cut(s) 539
BstSCI CCNGG 7 cut(s) 286, 388, 471, 641, 1666, 2325, 2651
BstSFI CTRYAG 3 cut(s) 977, 2370, 2789
BstUI CGCG 1 cut(s) 3018
BstV1I GCAGC 4 cut(s) 269, 814, 963, 3011
BstV2I GAAGAC 2 cut(s) 1731, 2999
BstX2I RGATCY 3 cut(s) 305, 1024, 1111
BstXI CCANNNNNNTGG 2 cut(s) 649, 676
BstYI RGATCY 3 cut(s) 305, 1024, 1111
Bsu15I ATCGAT 1 cut(s) 3190
Bsu36I CCTNAGG 3 cut(s) 897, 1710, 2451
BsuI GTATCC 1 cut(s) 2956
BsuRI GGCC 3 cut(s) 1001, 1229, 1917
BsuTUI ATCGAT 1 cut(s) 3190
BtrI CACGTC 2 cut(s) 483, 2671
BtsCI GGATG 6 cut(s) 748, 839, 1066, 2359, 2461, 2497
BtsI GCAGTG 1 cut(s) 2338
BtsIMutI CAGTG 9 cut(s) 64, 168, 199, 447, 669, 1301, 1740, 2338, 2906
BveI ACCTGC 2 cut(s) 527, 1084
Cac8I GCNNGC 6 cut(s) 818, 2069, 2226, 2621, 2735, 2926
CaiI CAGNNNCTG 1 cut(s) 2534
CciI TCATGA 1 cut(s) 3076
Cfr13I GGNCC 3 cut(s) 781, 999, 1996
ClaI ATCGAT 1 cut(s) 3190
CsiI ACCWGGT 1 cut(s) 388
Csp6I GTAC 5 cut(s) 108, 967, 1974, 2047, 2943
CspCI CAANNNNNGTGG 2 cut(s) 1333, 1368
CviQI GTAC 5 cut(s) 108, 967, 1974, 2047, 2943
DpnI GATC 8 cut(s) 52, 307, 1026, 1113, 1584, 1810, 2694, 3189
DpnII GATC 8 cut(s) 50, 305, 1024, 1111, 1582, 1808, 2692, 3187
DraI TTTAAA 1 cut(s) 2155
DraIII CACNNNGTG 2 cut(s) 2674, 2903
Ecl136II GAGCTC 2 cut(s) 2267, 2797
Eco130I CCWWGG 2 cut(s) 297, 1822
Eco24I GRGCYC 2 cut(s) 2269, 2799
Eco32I GATATC 2 cut(s) 803, 1881
Eco47I GGWCC 2 cut(s) 781, 1996
Eco53kI GAGCTC 2 cut(s) 2267, 2797
Eco57I CTGAAG 3 cut(s) 1647, 2609, 2823
Eco72I CACGTG 1 cut(s) 2114
Eco81I CCTNAGG 3 cut(s) 897, 1710, 2451
Eco91I GGTNACC 1 cut(s) 539
EcoICRI GAGCTC 2 cut(s) 2267, 2797
EcoNI CCTNNNNNAGG 1 cut(s) 393
EcoO109I RGGNCCY 1 cut(s) 781
EcoO65I GGTNACC 1 cut(s) 539
EcoRII CCWGG 7 cut(s) 286, 388, 471, 641, 1666, 2325, 2651
EcoRV GATATC 2 cut(s) 803, 1881
EcoT14I CCWWGG 2 cut(s) 297, 1822
EcoT22I ATGCAT 1 cut(s) 3074
EcoT38I GRGCYC 2 cut(s) 2269, 2799
ErhI CCWWGG 2 cut(s) 297, 1822
Esp3I CGTCTC 1 cut(s) 189
FalI AAGNNNNNCTT 2 cut(s) 2033, 2065
FaqI GGGAC 3 cut(s) 596, 794, 1440
FbaI TGATCA 1 cut(s) 2692
FblI GTMKAC 1 cut(s) 1988
Fnu4HI GCNGC 5 cut(s) 258, 828, 977, 2922, 3025
FokI GGATG 6 cut(s) 755, 846, 1053, 2366, 2468, 2504
FriOI GRGCYC 2 cut(s) 2269, 2799
Fsp4HI GCNGC 5 cut(s) 258, 828, 977, 2922, 3025
GluI GCNGC 5 cut(s) 258, 828, 977, 2922, 3025
GsuI CTGGAG 1 cut(s) 2406
HaeIII GGCC 3 cut(s) 1001, 1229, 1917
HapII CCGG 2 cut(s) 1520, 2960
HincII GTYRAC 1 cut(s) 1989
HindII GTYRAC 1 cut(s) 1989
HindIII AAGCTT 1 cut(s) 927
HpaII CCGG 2 cut(s) 1520, 2960
HphI GGTGA 7 cut(s) 341, 551, 1480, 1634, 2602, 2735, 2858
Hpy166II GTNNAC 3 cut(s) 1989, 2714, 2897
Hpy8I GTNNAC 3 cut(s) 1989, 2714, 2897
Hpy99I CGWCG 1 cut(s) 2675
HpyAV CCTTC 6 cut(s) 41, 393, 897, 1798, 2617, 2798
HpyCH4IV ACGT 5 cut(s) 356, 482, 1861, 2113, 2670
HpyF10VI GCNNNNNNNGC 6 cut(s) 2502, 2511, 2636, 2826, 3024, 3119
HpySE526I ACGT 5 cut(s) 356, 482, 1861, 2113, 2670
KflI GGGWCCC 1 cut(s) 781
Kpn2I TCCGGA 1 cut(s) 1519
KpnI GGTACC 1 cut(s) 1977
Ksp22I TGATCA 1 cut(s) 2692
Kzo9I GATC 8 cut(s) 50, 305, 1024, 1111, 1582, 1808, 2692, 3187
LmnI GCTCC 4 cut(s) 176, 1288, 2794, 2802
Lsp1109I GCAGC 4 cut(s) 269, 814, 963, 3011
LweI GCATC 6 cut(s) 325, 1954, 2015, 2395, 2674, 3059
MabI ACCWGGT 1 cut(s) 388
MaeII ACGT 5 cut(s) 356, 482, 1861, 2113, 2670
MaeIII GTNAC 9 cut(s) 127, 208, 215, 403, 442, 478, 539, 1604, 2482
MalI GATC 8 cut(s) 52, 307, 1026, 1113, 1584, 1810, 2694, 3189
MboI GATC 8 cut(s) 50, 305, 1024, 1111, 1582, 1808, 2692, 3187
MfeI CAATTG 1 cut(s) 1836
MflI RGATCY 3 cut(s) 305, 1024, 1111
MhlI GDGCHC 6 cut(s) 66, 181, 1801, 2269, 2666, 2799
MlyI GAGTC 7 cut(s) 164, 223, 486, 2054, 2308, 2554, 2884
MmeI TCCRAC 2 cut(s) 1297, 2816
Mph1103I ATGCAT 1 cut(s) 3074
MroI TCCGGA 1 cut(s) 1519
MroXI GAANNNNTTC 2 cut(s) 2089, 2585
MslI CAYNNNNRTG 7 cut(s) 36, 674, 1578, 1647, 1733, 2116, 2155
MspA1I CMGCKG 1 cut(s) 2639
MspI CCGG 2 cut(s) 1520, 2960
MspR9I CCNGG 7 cut(s) 288, 390, 473, 643, 1668, 2327, 2653
MunI CAATTG 1 cut(s) 1836
Mva1269I GAATGC 6 cut(s) 1477, 1948, 2365, 3072, 3131, 3173
MvaI CCWGG 7 cut(s) 288, 390, 473, 643, 1668, 2327, 2653
MvnI CGCG 1 cut(s) 3018
MwoI GCNNNNNNNGC 6 cut(s) 2502, 2511, 2636, 2826, 3024, 3119
NdeII GATC 8 cut(s) 50, 305, 1024, 1111, 1582, 1808, 2692, 3187
NlaIV GGNNCC 4 cut(s) 782, 783, 1975, 2953
NmuCI GTSAC 5 cut(s) 215, 403, 442, 478, 539
NsiI ATGCAT 1 cut(s) 3074
NspI RCATGY 3 cut(s) 512, 2228, 2928
NspV TTCGAA 1 cut(s) 1202
OliI CACNNNNGTG 1 cut(s) 1733
PaeI GCATGC 2 cut(s) 2228, 2928
PagI TCATGA 1 cut(s) 3076
PaqCI CACCTGC 1 cut(s) 527
PctI GAATGC 6 cut(s) 1477, 1948, 2365, 3072, 3131, 3173
PdmI GAANNNNTTC 2 cut(s) 2089, 2585
PflMI CCANNNNNTGG 2 cut(s) 930, 1538
PkrI GCNGC 5 cut(s) 259, 829, 978, 2923, 3026
PleI GAGTC 7 cut(s) 163, 222, 485, 2054, 2307, 2553, 2883
PmaCI CACGTG 1 cut(s) 2114
PmlI CACGTG 1 cut(s) 2114
PpsI GAGTC 7 cut(s) 163, 222, 485, 2054, 2307, 2553, 2883
Ppu21I YACGTR 2 cut(s) 1862, 2114
PpuMI RGGWCCY 1 cut(s) 781
PshAI GACNNNNGTC 3 cut(s) 102, 786, 1994
PshBI ATTAAT 1 cut(s) 3030
PsiI TTATAA 2 cut(s) 252, 2198
Psp124BI GAGCTC 2 cut(s) 2269, 2799
Psp5II RGGWCCY 1 cut(s) 781
Psp6I CCWGG 7 cut(s) 286, 388, 471, 641, 1666, 2325, 2651
PspCI CACGTG 1 cut(s) 2114
PspEI GGTNACC 1 cut(s) 539
PspGI CCWGG 7 cut(s) 286, 388, 471, 641, 1666, 2325, 2651
PspN4I GGNNCC 4 cut(s) 782, 783, 1975, 2953
PspPI GGNCC 3 cut(s) 781, 999, 1996
PspPPI RGGWCCY 1 cut(s) 781
PsrI GAACNNNNNNTAC 2 cut(s) 1357, 1389
PstI CTGCAG 2 cut(s) 981, 2793
PstNI CAGNNNCTG 1 cut(s) 2534
PsuI RGATCY 3 cut(s) 305, 1024, 1111
PvuII CAGCTG 1 cut(s) 2639
RsaI GTAC 5 cut(s) 109, 968, 1975, 2048, 2944
RsaNI GTAC 5 cut(s) 108, 967, 1974, 2047, 2943
RseI CAYNNNNRTG 7 cut(s) 36, 674, 1578, 1647, 1733, 2116, 2155
SacI GAGCTC 2 cut(s) 2269, 2799
SalI GTCGAC 1 cut(s) 1987
SatI GCNGC 5 cut(s) 258, 828, 977, 2922, 3025
Sau3AI GATC 8 cut(s) 50, 305, 1024, 1111, 1582, 1808, 2692, 3187
Sau96I GGNCC 3 cut(s) 781, 999, 1996
SchI GAGTC 7 cut(s) 164, 223, 486, 2054, 2308, 2554, 2884
ScrFI CCNGG 7 cut(s) 288, 390, 473, 643, 1668, 2327, 2653
SduI GDGCHC 6 cut(s) 66, 181, 1801, 2269, 2666, 2799
SexAI ACCWGGT 1 cut(s) 388
SfaNI GCATC 6 cut(s) 325, 1954, 2015, 2395, 2674, 3059
SfcI CTRYAG 3 cut(s) 977, 2370, 2789
SfuI TTCGAA 1 cut(s) 1202
SinI GGWCC 2 cut(s) 781, 1996
SmiI ATTTAAAT 1 cut(s) 2155
SmiMI CAYNNNNRTG 7 cut(s) 36, 674, 1578, 1647, 1733, 2116, 2155
SpeI ACTAGT 2 cut(s) 110, 1235
SphI GCATGC 2 cut(s) 2228, 2928
SsiI CCGC 4 cut(s) 791, 934, 1389, 2921
SspI AATATT 2 cut(s) 1048, 2234
SstI GAGCTC 2 cut(s) 2269, 2799
StyD4I CCNGG 7 cut(s) 286, 388, 471, 641, 1666, 2325, 2651
StyI CCWWGG 2 cut(s) 297, 1822
SwaI ATTTAAAT 1 cut(s) 2155
TaiI ACGT 5 cut(s) 359, 485, 1864, 2116, 2673
TaqI TCGA 7 cut(s) 53, 1202, 1576, 1988, 2822, 2873, 3190
TatI WGTACW 1 cut(s) 2046
TauI GCSGC 1 cut(s) 2924
TscAI CASTG 9 cut(s) 71, 175, 199, 447, 676, 1308, 1740, 2338, 2906
TseFI GTSAC 5 cut(s) 215, 403, 442, 478, 539
TseI GCWGC 4 cut(s) 257, 827, 976, 3024
Tsp45I GTSAC 5 cut(s) 215, 403, 442, 478, 539
TspGWI ACGGA 2 cut(s) 735, 2964
TspRI CASTG 9 cut(s) 71, 175, 199, 447, 676, 1308, 1740, 2338, 2906
Van91I CCANNNNNTGG 2 cut(s) 930, 1538
VpaK11BI GGWCC 2 cut(s) 781, 1996
VspI ATTAAT 1 cut(s) 3030
XagI CCTNNNNNAGG 1 cut(s) 393
XapI RAATTY 4 cut(s) 948, 1415, 1660, 2006
XbaI TCTAGA 2 cut(s) 1595, 2240
XceI RCATGY 3 cut(s) 512, 2228, 2928
XcmI CCANNNNNNNNNTGG 1 cut(s) 2075
XmiI GTMKAC 1 cut(s) 1988
XmnI GAANNNNTTC 2 cut(s) 2089, 2585
Zsp2I ATGCAT 1 cut(s) 3074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.