pycom03g19640

salt tolerance-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0015021
Physical Location & Seq
Forward (+)
20243856 .. 20245299
1444 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 873 bp
ATGAAGATACAGTGCAACGTCTGCGAGGCAGCGGAGGCGAACGTCCTCTGCTGCGCCGACGAGGCGGCTCTCTGCTGGGCGTGCGACGAGAAGGTTCACAAAGCGAACAAGCTCGCGAGTAAGCACCAGAGGGTTCGTCTTTCCTCCTCCCACTTGCCCAAATGCGATATCTGCCAGGAGGCAGTTGGGTACTTTTTCTGCTTAGAAGATCGAGCTTTGCTATGTAGAAAATGTGATGTTGCCGTTCACACGGCTAATAGCTTCGTGTCAGCTCACCGGAGGTTTTTGCTTACCGGAATCAAGGTTGGGCCTGAGCCGGCTCAGCCGGATTTCGGCGACGGTGATGGGGTTGGTGCGTCTTCTTCTACTATGAAGTCACGTCCTGGGTCTGGTTCTGGCTCAGTGTCTAGATGTGAAACGCACGACTCGTTGCCGGTGGAGTGCAAGGCGGCGCTGCCCGGAGTTGCCGGGGGGATGTCGTTTACTGGGGGCTCTTCAGCTGGGACGGTTCCACAATGTTATATAGATGAGTTTCTTGGACTCTCTGATCTTGATCAGAGCTTTGGCTACATTGATAATGGATCTTCAAAGGCTGACTGCGGTAAACTTGGGGAATATGGCTCACCAGTTTTAAGATCGTCTGAGGAGGAACTGGACGATTATGAGTGCATAGGTGAGGTGCCGGAGGCCTCATGGATGGTACCACAGATACCTTCCCCACCTACAGCATCGGGACTATACTGGCCAAAAAGTCTTCAGAATCCGTCGGATTCTTCTACAATGTTTGTTCCAGACATCTGCCACTCACAAATGGAAAACCCTTTTTGTTCCCAACATAATGGTACTGTCTCAAAACGTCGTAGGCAGTTCTAG

Protein Analysis

291

Amino Acids

31.12

Weight (kDa)

5.28

Isoelectric Point (pI)

55.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 53 - 91 1.4e-06 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015021)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10240
fragaria_vesca FvH4_3g17750
malus_domestica MD03G1247700.v1.1 MD11G1269800.v1.1
prunus_persica Prupe.4G156600_v2.0.a1
pyrus_communis pycom03g19640 pycom11g23830
rosa_chinensis RchiOBHm_Chr5g0029541
rosa_laevigata RLG00000033177
rosa_multiflora Rmu_sc0000210.1_g000014
rosa_roxburghii Rroxscaffold_1G00050400
rosa_samantha Rh5AG206900 Rh5BG205600 Rh5CG227700 Rh5DG209300
rosa_wichuraiana Rw5G018820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 700
AccB1I GGYRCC 2 cut(s) 679, 700
AccII CGCG 1 cut(s) 116
AciI CCGC 4 cut(s) 32, 65, 449, 600
AclWI GGATC 1 cut(s) 589
AcoI YGGCCR 1 cut(s) 743
AcuI CTGAAG 2 cut(s) 480, 740
AfaI GTAC 3 cut(s) 191, 702, 844
AfiI CCNNNNNNNGG 2 cut(s) 332, 389
AgsI TTSAA 1 cut(s) 588
AjiI CACGTC 1 cut(s) 380
AjnI CCWGG 2 cut(s) 174, 382
AluBI AGCT 6 cut(s) 112, 215, 261, 272, 500, 561
AluI AGCT 6 cut(s) 112, 215, 261, 272, 500, 561
Alw26I GTCTC 1 cut(s) 853
AlwI GGATC 1 cut(s) 589
AoxI GGCC 3 cut(s) 308, 687, 743
ApeKI GCWGC 3 cut(s) 29, 51, 454
Asp718I GGTACC 1 cut(s) 700
AspLEI GCGC 2 cut(s) 56, 454
AspS9I GGNCC 1 cut(s) 308
AsuC2I CCSGG 2 cut(s) 459, 469
AsuHPI GGTGA 4 cut(s) 266, 353, 615, 686
BalI TGGCCA 1 cut(s) 745
BanI GGYRCC 2 cut(s) 679, 700
BanII GRGCYC 1 cut(s) 494
BbsI GAAGAC 2 cut(s) 351, 746
BbvI GCAGC 3 cut(s) 38, 41, 441
BccI CCATC 2 cut(s) 338, 691
BceAI ACGGC 2 cut(s) 227, 267
BciT130I CCWGG 2 cut(s) 176, 384
BclI TGATCA 1 cut(s) 553
BcnI CCSGG 2 cut(s) 459, 469
BcoDI GTCTC 1 cut(s) 853
BfaI CTAG 2 cut(s) 408, 871
BfmI CTRYAG 1 cut(s) 723
BfoI RGCGCY 1 cut(s) 455
BglI GCCNNNNNGGC 1 cut(s) 62
BisI GCNGC 5 cut(s) 30, 52, 66, 450, 455
BlpI GCTNAGC 1 cut(s) 321
BlsI GCNGC 5 cut(s) 31, 53, 67, 451, 456
Bme1390I CCNGG 4 cut(s) 176, 384, 459, 469
BmgBI CACGTC 1 cut(s) 380
BmgT120I GGNCC 1 cut(s) 308
BmiI GGNNCC 3 cut(s) 510, 681, 702
BmrFI CCNGG 4 cut(s) 176, 384, 459, 469
BmrI ACTGGG 1 cut(s) 495
BmsI GCATC 1 cut(s) 737
BmuI ACTGGG 1 cut(s) 495
BpiI GAAGAC 2 cut(s) 351, 746
Bpu10I CCTNAGC 1 cut(s) 312
Bpu1102I GCTNAGC 1 cut(s) 321
BpuMI CCSGG 2 cut(s) 459, 469
BsaBI GATNNNNATC 1 cut(s) 552
BsaJI CCNNGG 2 cut(s) 383, 468
BsaWI WCCGGW 2 cut(s) 276, 293
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 389
Bse118I RCCGGY 2 cut(s) 316, 433
Bse1I ACTGG 4 cut(s) 490, 626, 657, 746
Bse8I GATNNNNATC 1 cut(s) 552
BseBI CCWGG 2 cut(s) 176, 384
BseDI CCNNGG 2 cut(s) 383, 468
BseGI GGATG 2 cut(s) 480, 702
BseJI GATNNNNATC 1 cut(s) 552
BseLI CCNNNNNNNGG 2 cut(s) 332, 389
BseMII CTCAG 4 cut(s) 303, 335, 414, 633
BseNI ACTGG 4 cut(s) 490, 626, 657, 746
BseRI GAGGAG 2 cut(s) 136, 659
BseXI GCAGC 3 cut(s) 38, 41, 441
BseYI CCCAGC 2 cut(s) 75, 500
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 3 cut(s) 310, 689, 745
BshNI GGYRCC 2 cut(s) 679, 700
BsiSI CCGG 8 cut(s) 277, 294, 317, 326, 434, 459, 468, 683
BslFI GGGAC 2 cut(s) 517, 747
BslI CCNNNNNNNGG 2 cut(s) 332, 389
BsmAI GTCTC 1 cut(s) 853
BsmFI GGGAC 2 cut(s) 517, 747
BsnI GGCC 3 cut(s) 310, 689, 745
Bsp1286I GDGCHC 1 cut(s) 494
Bsp143I GATC 5 cut(s) 208, 547, 553, 581, 635
Bsp1720I GCTNAGC 1 cut(s) 321
Bsp68I TCGCGA 1 cut(s) 116
BspACI CCGC 4 cut(s) 32, 65, 449, 600
BspANI GGCC 3 cut(s) 310, 689, 745
BspCNI CTCAG 4 cut(s) 304, 334, 413, 634
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 3 cut(s) 510, 681, 702
BspPI GGATC 1 cut(s) 589
BspQI GCTCTTC 1 cut(s) 499
BspT107I GGYRCC 2 cut(s) 679, 700
BsrFI RCCGGY 2 cut(s) 316, 433
BsrI ACTGG 4 cut(s) 490, 626, 657, 746
BssAI RCCGGY 2 cut(s) 316, 433
BssECI CCNNGG 2 cut(s) 383, 468
BssMI GATC 5 cut(s) 208, 547, 553, 581, 635
Bst2UI CCWGG 2 cut(s) 176, 384
Bst4CI ACNGT 4 cut(s) 12, 341, 508, 847
Bst6I CTCTTC 1 cut(s) 499
BstAPI GCANNNNNTGC 1 cut(s) 21
BstC8I GCNNGC 3 cut(s) 82, 114, 318
BstDEI CTNAG 5 cut(s) 202, 312, 321, 400, 642
BstF5I GGATG 2 cut(s) 480, 702
BstFNI CGCG 1 cut(s) 116
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 2 cut(s) 56, 454
BstKTI GATC 5 cut(s) 211, 550, 556, 584, 638
BstMAI GTCTC 1 cut(s) 853
BstMBI GATC 5 cut(s) 208, 547, 553, 581, 635
BstMWI GCNNNNNNNGC 6 cut(s) 21, 35, 62, 81, 171, 322
BstNI CCWGG 2 cut(s) 176, 384
BstSCI CCNGG 4 cut(s) 174, 382, 457, 467
BstSFI CTRYAG 1 cut(s) 723
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 3 cut(s) 38, 41, 441
BstV2I GAAGAC 2 cut(s) 351, 746
BstX2I RGATCY 1 cut(s) 581
BstXI CCANNNNNNTGG 1 cut(s) 839
BstYI RGATCY 1 cut(s) 581
BsuRI GGCC 3 cut(s) 310, 689, 745
BtrI CACGTC 1 cut(s) 380
BtsCI GGATG 2 cut(s) 480, 702
BtsIMutI CAGTG 2 cut(s) 17, 408
BtuMI TCGCGA 1 cut(s) 116
Cac8I GCNNGC 3 cut(s) 82, 114, 318
CfoI GCGC 2 cut(s) 56, 454
Cfr10I RCCGGY 2 cut(s) 316, 433
Cfr13I GGNCC 1 cut(s) 308
CseI GACGC 1 cut(s) 345
Csp6I GTAC 3 cut(s) 190, 701, 843
CviAII CATG 1 cut(s) 693
CviQI GTAC 3 cut(s) 190, 701, 843
DdeI CTNAG 5 cut(s) 202, 312, 321, 400, 642
DpnI GATC 5 cut(s) 210, 549, 555, 583, 637
DpnII GATC 5 cut(s) 208, 547, 553, 581, 635
EaeI YGGCCR 1 cut(s) 743
Eam1104I CTCTTC 1 cut(s) 499
EarI CTCTTC 1 cut(s) 499
Eco147I AGGCCT 1 cut(s) 689
Eco24I GRGCYC 1 cut(s) 494
Eco32I GATATC 1 cut(s) 169
Eco57I CTGAAG 2 cut(s) 480, 740
EcoRII CCWGG 2 cut(s) 174, 382
EcoRV GATATC 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 494
FaeI CATG 1 cut(s) 696
FaqI GGGAC 2 cut(s) 517, 747
FatI CATG 1 cut(s) 692
FbaI TGATCA 1 cut(s) 553
Fnu4HI GCNGC 5 cut(s) 30, 52, 66, 450, 455
FokI GGATG 2 cut(s) 487, 709
FriOI GRGCYC 1 cut(s) 494
Fsp4HI GCNGC 5 cut(s) 30, 52, 66, 450, 455
FspBI CTAG 2 cut(s) 408, 871
GlaI GCGC 2 cut(s) 55, 453
GluI GCNGC 5 cut(s) 30, 52, 66, 450, 455
GsaI CCCAGC 2 cut(s) 79, 504
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 3 cut(s) 310, 689, 745
HapII CCGG 8 cut(s) 277, 294, 317, 326, 434, 459, 468, 683
HgaI GACGC 1 cut(s) 345
HhaI GCGC 2 cut(s) 56, 454
Hin1II CATG 1 cut(s) 696
Hin6I GCGC 2 cut(s) 54, 452
HinP1I GCGC 2 cut(s) 54, 452
HinfI GANTC 5 cut(s) 297, 425, 540, 760, 770
HpaII CCGG 8 cut(s) 277, 294, 317, 326, 434, 459, 468, 683
HphI GGTGA 4 cut(s) 266, 353, 615, 686
Hpy166II GTNNAC 4 cut(s) 97, 247, 483, 605
Hpy188I TCNGA 5 cut(s) 547, 558, 643, 759, 769
Hpy188III TCNNGA 5 cut(s) 115, 408, 551, 732, 791
Hpy8I GTNNAC 4 cut(s) 97, 247, 483, 605
Hpy99I CGWCG 5 cut(s) 62, 89, 341, 769, 861
HpyAV CCTTC 2 cut(s) 85, 723
HpyCH4III ACNGT 4 cut(s) 12, 341, 508, 847
HpyCH4IV ACGT 4 cut(s) 18, 42, 379, 856
HpyCH4V TGCA 3 cut(s) 15, 444, 669
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 35, 62, 81, 171, 322
HpyF3I CTNAG 5 cut(s) 202, 312, 321, 400, 642
HpySE526I ACGT 4 cut(s) 18, 42, 379, 856
Hsp92II CATG 1 cut(s) 696
HspAI GCGC 2 cut(s) 54, 452
KpnI GGTACC 1 cut(s) 704
KroI GCCGGC 1 cut(s) 316
KroNI GCCGGC 1 cut(s) 318
Ksp22I TGATCA 1 cut(s) 553
Kzo9I GATC 5 cut(s) 208, 547, 553, 581, 635
LguI GCTCTTC 1 cut(s) 499
Lsp1109I GCAGC 3 cut(s) 38, 41, 441
LweI GCATC 1 cut(s) 737
MaeI CTAG 2 cut(s) 408, 871
MaeII ACGT 4 cut(s) 18, 42, 379, 856
MaeIII GTNAC 1 cut(s) 375
MalI GATC 5 cut(s) 210, 549, 555, 583, 637
MboI GATC 5 cut(s) 208, 547, 553, 581, 635
MboII GAAGA 8 cut(s) 16, 218, 351, 354, 486, 576, 746, 765
MflI RGATCY 1 cut(s) 581
MhlI GDGCHC 1 cut(s) 494
MlsI TGGCCA 1 cut(s) 745
MluNI TGGCCA 1 cut(s) 745
MlyI GAGTC 2 cut(s) 419, 534
MmeI TCCRAC 1 cut(s) 747
Mox20I TGGCCA 1 cut(s) 745
MroNI GCCGGC 1 cut(s) 316
MscI TGGCCA 1 cut(s) 745
MseI TTAA 1 cut(s) 632
Msp20I TGGCCA 1 cut(s) 745
MspA1I CMGCKG 2 cut(s) 32, 500
MspI CCGG 8 cut(s) 277, 294, 317, 326, 434, 459, 468, 683
MspR9I CCNGG 4 cut(s) 176, 384, 459, 469
MvaI CCWGG 2 cut(s) 176, 384
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 6 cut(s) 21, 35, 62, 81, 171, 322
NaeI GCCGGC 1 cut(s) 318
NciI CCSGG 2 cut(s) 459, 469
NdeII GATC 5 cut(s) 208, 547, 553, 581, 635
NgoMIV GCCGGC 1 cut(s) 316
NlaIII CATG 1 cut(s) 696
NlaIV GGNNCC 3 cut(s) 510, 681, 702
NmuCI GTSAC 1 cut(s) 375
NruI TCGCGA 1 cut(s) 116
PceI AGGCCT 1 cut(s) 689
PciSI GCTCTTC 1 cut(s) 499
PcsI WCGNNNNNNNCGW 1 cut(s) 425
PdiI GCCGGC 1 cut(s) 318
PfeI GAWTC 3 cut(s) 297, 760, 770
PkrI GCNGC 5 cut(s) 31, 53, 67, 451, 456
PleI GAGTC 2 cut(s) 419, 534
PpsI GAGTC 2 cut(s) 419, 534
Psp6I CCWGG 2 cut(s) 174, 382
PspFI CCCAGC 2 cut(s) 75, 500
PspGI CCWGG 2 cut(s) 174, 382
PspN4I GGNNCC 3 cut(s) 510, 681, 702
PspPI GGNCC 1 cut(s) 308
PsuI RGATCY 1 cut(s) 581
PvuII CAGCTG 1 cut(s) 500
RruI TCGCGA 1 cut(s) 116
RsaI GTAC 3 cut(s) 191, 702, 844
RsaNI GTAC 3 cut(s) 190, 701, 843
SapI GCTCTTC 1 cut(s) 499
SaqAI TTAA 1 cut(s) 632
SatI GCNGC 5 cut(s) 30, 52, 66, 450, 455
Sau3AI GATC 5 cut(s) 208, 547, 553, 581, 635
Sau96I GGNCC 1 cut(s) 308
SchI GAGTC 2 cut(s) 419, 534
ScrFI CCNGG 4 cut(s) 176, 384, 459, 469
SduI GDGCHC 1 cut(s) 494
SfaNI GCATC 1 cut(s) 737
SfcI CTRYAG 1 cut(s) 723
SseBI AGGCCT 1 cut(s) 689
SsiI CCGC 4 cut(s) 32, 65, 449, 600
SspMI CTAG 2 cut(s) 408, 871
StuI AGGCCT 1 cut(s) 689
StyD4I CCNGG 4 cut(s) 174, 382, 457, 467
TaaI ACNGT 4 cut(s) 12, 341, 508, 847
TaiI ACGT 4 cut(s) 21, 45, 382, 859
TaqI TCGA 1 cut(s) 211
TauI GCSGC 2 cut(s) 68, 452
TfiI GAWTC 3 cut(s) 297, 760, 770
Tru1I TTAA 1 cut(s) 632
Tru9I TTAA 1 cut(s) 632
TscAI CASTG 2 cut(s) 17, 408
TseFI GTSAC 1 cut(s) 375
TseI GCWGC 3 cut(s) 29, 51, 454
Tsp45I GTSAC 1 cut(s) 375
TspDTI ATGAA 2 cut(s) 17, 386
TspGWI ACGGA 1 cut(s) 753
TspRI CASTG 2 cut(s) 17, 408
XbaI TCTAGA 1 cut(s) 407
XcmI CCANNNNNNNNNTGG 1 cut(s) 182
XspI CTAG 2 cut(s) 408, 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.