pycom03g20230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005344
Physical Location & Seq
Reverse (-)
20752387 .. 20752737
351 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g20230.1

Sequence Viewer

Length: 351 bp
ATGGAATTTTCATGGATAATCTTCATGAATTTGGTAGCATTAACCAAGAGGATCGATTTTCAGCTGTTCGATGTTGGATCTGTGGACGGTAGGGATTTCCAGGAAACAAACGCACCTGGGTTCGGTTGGATGGATATGGAAATATGGTTTTCTTCTTCACAGGGTACTGATGAATCAGTTGATTGGTACATGGATGATTCTTGTTTTGGTGACATCGATCGCATGTCTAGTTCTGGAGGGTCTCAGTACAATGGCTGCATTACTACAACTAATACTCCTTCTGAGGAAGATGGACACCAAAATCTGTGGCAGGAAACGTATGTTAATACAAGGATGCATATGTATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

117

Amino Acids

13.4

Weight (kDa)

4.05

Isoelectric Point (pI)

53.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 59, 85
AcsI RAATTY 2 cut(s) 5, 28
AfaI GTAC 3 cut(s) 166, 188, 248
AfiI CCNNNNNNNGG 1 cut(s) 122
AjnI CCWGG 2 cut(s) 99, 115
AluBI AGCT 1 cut(s) 64
AluI AGCT 1 cut(s) 64
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 2 cut(s) 59, 85
ApeKI GCWGC 1 cut(s) 255
ApoI RAATTY 2 cut(s) 5, 28
AsuHPI GGTGA 1 cut(s) 221
BbvI GCAGC 1 cut(s) 242
BccI CCATC 2 cut(s) 124, 284
BciT130I CCWGG 2 cut(s) 101, 117
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 1 cut(s) 228
BisI GCNGC 1 cut(s) 256
BlsI GCNGC 1 cut(s) 257
Bme1390I CCNGG 2 cut(s) 101, 117
BmrFI CCNGG 2 cut(s) 101, 117
BmsI GCATC 1 cut(s) 324
BpmI CTGGAG 1 cut(s) 255
Bsa29I ATCGAT 2 cut(s) 54, 216
BsaI GGTCTC 1 cut(s) 246
BsaJI CCNNGG 1 cut(s) 116
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bsc4I CCNNNNNNNGG 1 cut(s) 122
BseBI CCWGG 2 cut(s) 101, 117
BseCI ATCGAT 2 cut(s) 54, 216
BseDI CCNNGG 1 cut(s) 116
BseGI GGATG 3 cut(s) 135, 199, 339
BseLI CCNNNNNNNGG 1 cut(s) 122
BseMII CTCAG 2 cut(s) 257, 273
BseXI GCAGC 1 cut(s) 242
Bsh1285I CGRYCG 1 cut(s) 220
BshVI ATCGAT 2 cut(s) 54, 216
BsiEI CGRYCG 1 cut(s) 220
BslI CCNNNNNNNGG 1 cut(s) 122
BsmAI GTCTC 1 cut(s) 246
Bso31I GGTCTC 1 cut(s) 246
Bsp143I GATC 3 cut(s) 51, 77, 217
BspCNI CTCAG 2 cut(s) 256, 274
BspDI ATCGAT 2 cut(s) 54, 216
BspHI TCATGA 1 cut(s) 24
BspPI GGATC 2 cut(s) 59, 85
BspTNI GGTCTC 1 cut(s) 246
BssECI CCNNGG 1 cut(s) 116
BssMI GATC 3 cut(s) 51, 77, 217
Bst2UI CCWGG 2 cut(s) 101, 117
Bst4CI ACNGT 1 cut(s) 89
BstDEI CTNAG 2 cut(s) 243, 282
BstF5I GGATG 3 cut(s) 135, 199, 339
BstKTI GATC 3 cut(s) 54, 80, 220
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 3 cut(s) 51, 77, 217
BstMCI CGRYCG 1 cut(s) 220
BstNI CCWGG 2 cut(s) 101, 117
BstNSI RCATGY 1 cut(s) 226
BstSCI CCNGG 2 cut(s) 99, 115
BstV1I GCAGC 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 77
BstYI RGATCY 1 cut(s) 77
Bsu15I ATCGAT 2 cut(s) 54, 216
BsuTUI ATCGAT 2 cut(s) 54, 216
BtsCI GGATG 3 cut(s) 135, 199, 339
CciI TCATGA 1 cut(s) 24
ClaI ATCGAT 2 cut(s) 54, 216
Csp6I GTAC 3 cut(s) 165, 187, 247
CspCI CAANNNNNGTGG 2 cut(s) 287, 322
CviAII CATG 4 cut(s) 12, 25, 190, 223
CviJI RGCY 2 cut(s) 64, 255
CviKI_1 RGCY 2 cut(s) 64, 255
CviQI GTAC 3 cut(s) 165, 187, 247
DdeI CTNAG 2 cut(s) 243, 282
DpnI GATC 3 cut(s) 53, 79, 219
DpnII GATC 3 cut(s) 51, 77, 217
Eco31I GGTCTC 1 cut(s) 246
EcoRII CCWGG 2 cut(s) 99, 115
EcoT22I ATGCAT 1 cut(s) 339
FaeI CATG 4 cut(s) 15, 28, 193, 226
FatI CATG 4 cut(s) 11, 24, 189, 222
FauNDI CATATG 1 cut(s) 339
Fnu4HI GCNGC 1 cut(s) 256
FokI GGATG 3 cut(s) 142, 206, 346
Fsp4HI GCNGC 1 cut(s) 256
FspBI CTAG 1 cut(s) 228
GluI GCNGC 1 cut(s) 256
GsuI CTGGAG 1 cut(s) 255
Hin1II CATG 4 cut(s) 15, 28, 193, 226
HinfI GANTC 2 cut(s) 173, 197
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 1 cut(s) 283
Hpy188III TCNNGA 2 cut(s) 25, 234
Hpy8I GTNNAC 1 cut(s) 85
HpyAV CCTTC 1 cut(s) 288
HpyCH4III ACNGT 1 cut(s) 89
HpyCH4IV ACGT 1 cut(s) 317
HpyCH4V TGCA 2 cut(s) 258, 337
HpyF3I CTNAG 2 cut(s) 243, 282
HpySE526I ACGT 1 cut(s) 317
Hsp92II CATG 4 cut(s) 15, 28, 193, 226
Kzo9I GATC 3 cut(s) 51, 77, 217
LpnPI CCDG 7 cut(s) 86, 102, 113, 129, 146, 219, 296
Lsp1109I GCAGC 1 cut(s) 242
LweI GCATC 1 cut(s) 324
MaeI CTAG 1 cut(s) 228
MaeII ACGT 1 cut(s) 317
MaeIII GTNAC 1 cut(s) 209
MalI GATC 3 cut(s) 53, 79, 219
MboI GATC 3 cut(s) 51, 77, 217
MboII GAAGA 4 cut(s) 13, 144, 147, 299
MflI RGATCY 1 cut(s) 77
MluCI AATT 3 cut(s) 5, 28, 346
MmeI TCCRAC 2 cut(s) 55, 107
MnlI CCTC 3 cut(s) 42, 230, 277
Mph1103I ATGCAT 1 cut(s) 339
MseI TTAA 2 cut(s) 41, 324
MspA1I CMGCKG 1 cut(s) 64
MspR9I CCNGG 2 cut(s) 101, 117
MvaI CCWGG 2 cut(s) 101, 117
NdeI CATATG 1 cut(s) 339
NdeII GATC 3 cut(s) 51, 77, 217
NlaIII CATG 4 cut(s) 15, 28, 193, 226
NmuCI GTSAC 1 cut(s) 209
NsiI ATGCAT 1 cut(s) 339
NspI RCATGY 1 cut(s) 226
PagI TCATGA 1 cut(s) 24
PfeI GAWTC 2 cut(s) 173, 197
PfoI TCCNGGA 1 cut(s) 99
PkrI GCNGC 1 cut(s) 257
Ple19I CGATCG 1 cut(s) 220
Psp6I CCWGG 2 cut(s) 99, 115
PspGI CCWGG 2 cut(s) 99, 115
PsuI RGATCY 1 cut(s) 77
PvuI CGATCG 1 cut(s) 220
PvuII CAGCTG 1 cut(s) 64
RsaI GTAC 3 cut(s) 166, 188, 248
RsaNI GTAC 3 cut(s) 165, 187, 247
SaqAI TTAA 2 cut(s) 41, 324
SatI GCNGC 1 cut(s) 256
Sau3AI GATC 3 cut(s) 51, 77, 217
ScrFI CCNGG 2 cut(s) 101, 117
SetI ASST 3 cut(s) 66, 118, 320
SfaNI GCATC 1 cut(s) 324
Sse9I AATT 3 cut(s) 5, 28, 346
SspMI CTAG 1 cut(s) 228
StyD4I CCNGG 2 cut(s) 99, 115
TaaI ACNGT 1 cut(s) 89
TaiI ACGT 1 cut(s) 320
TaqI TCGA 3 cut(s) 54, 69, 216
TasI AATT 3 cut(s) 5, 28, 346
TatI WGTACW 1 cut(s) 246
TfiI GAWTC 2 cut(s) 173, 197
Tru1I TTAA 2 cut(s) 41, 324
Tru9I TTAA 2 cut(s) 41, 324
TseFI GTSAC 1 cut(s) 209
TseI GCWGC 1 cut(s) 255
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 3 cut(s) 13, 41, 186
XapI RAATTY 2 cut(s) 5, 28
XceI RCATGY 1 cut(s) 226
XspI CTAG 1 cut(s) 228
Zsp2I ATGCAT 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.