pycom03g23240

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005917
Physical Location & Seq
Forward (+)
23139231 .. 23141721
2491 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2241 bp
ATGAGGATTAAATGGTTTTTATTTGGACAAGTTTTTGGCATTTCATGGCTGCTGGCTTTGTTGACAATATCGGAATTATGTCACTATCGTGGTTGCATTCCTCGCCTTAAACTGTGCCTTATTACTCATGACTGTGATCGTTGTTATCAACCAAACATACAAACTGATATAGATACATTCATCGATATGAATCCGCATATTCCTACTGTCTCACTTGAGGTTCACTTGAGCTCTGAGAATATATTTGGTGAATTTGTTTGTTCATATATAATCCGGCATGTCCTCAGCGGCGGCGGTGGTTTGACTCCCCAGCTTGAAGATTATGAGAAGGAAGATCAAGAGCTCAAAGGGAAGCCAGACCATTTATTTGGGATAGAAGAATTAGGGCTGGGACAGATGGAAAAGTTATCTCATTTGTCCAAAGATCAACAACAATCCAAGGCAAGTTATTTGATGTTGGATAATTTCAGTTTCCATACAGGTTGTTCCGAACATGATCTCCAACGTTCCAGCTTCAAGTCCGAGAAAGCACGAGCTCATGTTTCCAAAGATCACGACCACCGCCACTACCAACAACAAAATCAACATTACCAACAACCATCCAAGTCAAATTACTCGGATTGGGATAATTTCACCTTTGACTCTGCCTTCCCATCCACGCAACCAATCCAGGACGCGACGAATGAGCTCACGTACAGCCAAATAGGAGAATCAGACATTGCGGAAACCAACAAGCAAATGCCCCATCAGCCTTCCTTAGCTGTGTTGGAGCTCCTAAACAACTACGGCAGTGCGTTCAAGAATCTGAGGGGAGACAGATTAAGCAACAGATGCAATGAAGCTGAAGCCTCCTTGTTTTCGATCCAACAAAAATTGTCAACCGAGGAGATTATGAGGGTTGCCGGAGCAAGGTATGTGCAATTCTCTAATCAAGGATATGGCGATTTTTTCATGCCTTTGCACCCGTTTGGGTACGCGCTTTCGGGTCTATCTGAAGAAGAAACGAAAGATGTGGAGCTTGCTCACATCCTTTTAGCTGCAGCAGAGAAGGTAGGCTACCAACAATTCGAGCGCGCAAACCGCCTGCTTCTTCGTTGTGAATTTGCTGCCTCCTTCAAAGTCAACCCTGTCCAGAGGATTGTTTTCTATTTCACCGAGGCCATGTATAAGGGGAATGAAATATCTACTTATAGTAATGGACTAAGCACAAACCTTACACTTCTTGCATTCCACCAAGATACCCCTTTTCATTCAGTAATGCAGCACCCAGGAATCCAAGTAATACTCGAAAACGTAGCCTTGGAAAGCAAGGTTCATTTGATTGATCGAAATCAGAAGCGGAGTGAGTGCCCCATTGAGCTCCTTACAATAACTGCAGTGGGAGTGACAGGCAAGCAGAAGATCGAGGAGACAGGAAGGAGGCTGGCAAGCGTCGCAAAGGCCTTGAACTTACCCTTTCAATTCAAGGCACTGATTGTGGCAGACATGGAAGATGTCAAGGACCAATTGTTTGATGTTGAGGATTACGAAGCTGTGGTGGTTTACGCGCCTCTAATACTAAGGACGATGATTTCGAGGCCAAGTTGCTTGGAAAATCTGATTAGGGTGATGAGAAATCTCAGCCCTCGTGTAATGGTGGTGATTGAGGTGGAGGCCAACCACAATTCACCATCATTTGTGAACCGATTCATCGACGCACTGTTTTATTACAGCGCATTTTTCGACTGCCTTGAGGCTTGCATGAAGCAGGAGAAGGACAGAGTGCTGATGGAGGGGTTGTTACATGAGGGAATACGAAACATTGTGGTGGCGGAGGGGAGTGAAAGGGTTGTGAGAAGCGTGACGATGGAGGTGTGGAGAGCCTTCTTTGCAAGGTTTAGAATGGTGGAAATCAACTTAAGTAATGCAGCTTTGTACCAAGCTAGTTTGGTGGCTAAAATGTTTGGAAGCCCTCCTTGCACACTTGATTGGAATGGGAAATGTCTTATTGTTGGGTGGAAAGGAACCCCAATCCATTCACTTTCGGCTTGGAACAAGTCATCCATACGACACTTCTCACAAAAGTGTAGAAGTCTCTCTATATTTAGTCTCACTCCTTATGAGAGGTACCCTTGTAGCGTAGAAATATCAAACTTGCGTCTCTTCGGTGAGAGAGGGAGGAGAAAGACTTGTATTTTCTATCCAAAGCGTCGTCGTTTTAATCAAACGATATTCGAATTTAAATTAATTTATTTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

747

Amino Acids

85.56

Weight (kDa)

6.51

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 341 - 677 9.7e-59 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2239
Acc65I GGTACC 1 cut(s) 2106
AccB1I GGYRCC 1 cut(s) 2106
AccII CGCG 4 cut(s) 677, 977, 1074, 1547
AciI CCGC 9 cut(s) 194, 288, 291, 294, 562, 722, 1081, 1339, 1811
AclI AACGTT 1 cut(s) 505
AclWI GGATC 1 cut(s) 856
AcsI RAATTY 3 cut(s) 251, 1100, 2216
AcuI CTGAAG 2 cut(s) 864, 1014
AfaI GTAC 4 cut(s) 695, 974, 1916, 2108
AfiI CCNNNNNNNGG 1 cut(s) 909
AflII CTTAAG 1 cut(s) 1897
AgsI TTSAA 7 cut(s) 317, 517, 799, 1117, 1447, 1460, 1465
AjnI CCWGG 2 cut(s) 669, 1267
AleI CACNNNNGTG 2 cut(s) 87, 2062
AloI GAACNNNNNNTCC 4 cut(s) 483, 515, 2024, 2056
Alw21I GWGCWC 6 cut(s) 233, 345, 538, 690, 774, 1362
Alw26I GTCTC 6 cut(s) 214, 807, 1403, 2078, 2093, 2144
AlwI GGATC 1 cut(s) 856
AoxI GGCC 4 cut(s) 1158, 1440, 1577, 1653
ApeKI GCWGC 6 cut(s) 49, 1037, 1040, 1106, 1261, 1907
ApoI RAATTY 3 cut(s) 251, 1100, 2216
ArsI GACNNNNNNTTYG 6 cut(s) 350, 382, 1493, 1525, 1555, 1587
AseI ATTAAT 1 cut(s) 2225
Asp700I GAANNNNTTC 1 cut(s) 1685
Asp718I GGTACC 1 cut(s) 2106
AspLEI GCGC 5 cut(s) 979, 1074, 1076, 1549, 1715
AspS9I GGNCC 1 cut(s) 1501
AsuHPI GGTGA 7 cut(s) 260, 625, 1144, 1618, 1651, 1659, 2159
AsuII TTCGAA 1 cut(s) 2214
AvaII GGWCC 1 cut(s) 1501
BaeGI GKGCMC 1 cut(s) 1352
BaeI ACNNNNGTAYC 2 cut(s) 2098, 2131
BanI GGYRCC 1 cut(s) 2106
BanII GRGCYC 6 cut(s) 233, 345, 538, 690, 774, 1362
BarI GAAGNNNNNNTAC 2 cut(s) 1040, 1072
BauI CACGAG 2 cut(s) 531, 1626
Bbv12I GWGCWC 6 cut(s) 233, 345, 538, 690, 774, 1362
BbvCI CCTCAGC 1 cut(s) 284
BbvI GCAGC 6 cut(s) 36, 1024, 1052, 1093, 1273, 1919
BccI CCATC 7 cut(s) 391, 607, 661, 753, 1678, 1762, 1840
BceAI ACGGC 1 cut(s) 802
BciT130I CCWGG 2 cut(s) 671, 1269
BcoDI GTCTC 6 cut(s) 214, 807, 1403, 2078, 2093, 2144
BfaI CTAG 1 cut(s) 1923
BfmI CTRYAG 2 cut(s) 1038, 1374
BfrI CTTAAG 1 cut(s) 1897
BisI GCNGC 8 cut(s) 50, 289, 292, 1038, 1041, 1107, 1262, 1908
BlsI GCNGC 8 cut(s) 51, 290, 293, 1039, 1042, 1108, 1263, 1909
Bme1390I CCNGG 2 cut(s) 671, 1269
Bme18I GGWCC 1 cut(s) 1501
BmgT120I GGNCC 1 cut(s) 1501
BmiI GGNNCC 2 cut(s) 2005, 2108
BmrFI CCNGG 2 cut(s) 671, 1269
BmsI GCATC 1 cut(s) 821
Bpu10I CCTNAGC 2 cut(s) 284, 757
Bpu14I TTCGAA 1 cut(s) 2214
BpuEI CTTGAG 3 cut(s) 236, 247, 1751
Bsa29I ATCGAT 1 cut(s) 183
BsaAI YACGTR 1 cut(s) 693
BsaBI GATNNNNATC 2 cut(s) 189, 1329
BsaJI CCNNGG 5 cut(s) 438, 882, 1155, 1267, 1299
BsaXI ACNNNNNCTCC 4 cut(s) 483, 513, 1763, 1793
Bsc4I CCNNNNNNNGG 1 cut(s) 909
Bse3DI GCAATG 2 cut(s) 717, 841
Bse8I GATNNNNATC 2 cut(s) 189, 1329
BseBI CCWGG 2 cut(s) 671, 1269
BseCI ATCGAT 1 cut(s) 183
BseDI CCNNGG 5 cut(s) 438, 882, 1155, 1267, 1299
BseGI GGATG 4 cut(s) 599, 653, 1026, 2039
BseJI GATNNNNATC 2 cut(s) 189, 1329
BseLI CCNNNNNNNGG 1 cut(s) 909
BseMI GCAATG 2 cut(s) 717, 841
BseMII CTCAG 4 cut(s) 225, 298, 797, 1633
BsePI GCGCGC 1 cut(s) 1072
BseRI GAGGAG 3 cut(s) 899, 1421, 2173
BseSI GKGCMC 1 cut(s) 1352
BseXI GCAGC 6 cut(s) 36, 1024, 1052, 1093, 1273, 1919
BseYI CCCAGC 2 cut(s) 309, 388
Bsh1236I CGCG 4 cut(s) 677, 977, 1074, 1547
BshFI GGCC 4 cut(s) 1160, 1442, 1579, 1655
BshNI GGYRCC 1 cut(s) 2106
BshVI ATCGAT 1 cut(s) 183
BsiHKAI GWGCWC 6 cut(s) 233, 345, 538, 690, 774, 1362
BsiSI CCGG 2 cut(s) 274, 903
BslFI GGGAC 1 cut(s) 405
BslI CCNNNNNNNGG 1 cut(s) 909
BsmAI GTCTC 6 cut(s) 214, 807, 1403, 2078, 2093, 2144
BsmBI CGTCTC 1 cut(s) 2144
BsmFI GGGAC 1 cut(s) 405
BsmI GAATGC 2 cut(s) 96, 1226
BsnI GGCC 4 cut(s) 1160, 1442, 1579, 1655
Bsp119I TTCGAA 1 cut(s) 2214
Bsp1286I GDGCHC 7 cut(s) 233, 345, 538, 690, 774, 1352, 1362
Bsp143I GATC 8 cut(s) 136, 334, 424, 496, 550, 861, 1324, 1401
BspACI CCGC 9 cut(s) 194, 288, 291, 294, 562, 722, 1081, 1339, 1811
BspANI GGCC 4 cut(s) 1160, 1442, 1579, 1655
BspCNI CTCAG 4 cut(s) 226, 297, 798, 1632
BspDI ATCGAT 1 cut(s) 183
BspFNI CGCG 4 cut(s) 677, 977, 1074, 1547
BspHI TCATGA 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 2005, 2108
BspMAI CTGCAG 2 cut(s) 1042, 1378
BspPI GGATC 1 cut(s) 856
BspT104I TTCGAA 1 cut(s) 2214
BspT107I GGYRCC 1 cut(s) 2106
BspTI CTTAAG 1 cut(s) 1897
BsrDI GCAATG 2 cut(s) 717, 841
BssECI CCNNGG 5 cut(s) 438, 882, 1155, 1267, 1299
BssHII GCGCGC 1 cut(s) 1072
BssMI GATC 8 cut(s) 136, 334, 424, 496, 550, 861, 1324, 1401
BssSI CACGAG 2 cut(s) 531, 1626
BssT1I CCWWGG 2 cut(s) 438, 1299
Bst2BI CACGAG 2 cut(s) 531, 1626
Bst2UI CCWGG 2 cut(s) 671, 1269
Bst4CI ACNGT 4 cut(s) 114, 134, 208, 1701
Bst6I CTCTTC 1 cut(s) 2147
BstAFI CTTAAG 1 cut(s) 1897
BstAPI GCANNNNNTGC 1 cut(s) 831
BstBAI YACGTR 1 cut(s) 693
BstBI TTCGAA 1 cut(s) 2214
BstC8I GCNNGC 8 cut(s) 54, 1020, 1074, 1085, 1394, 1425, 1429, 1738
BstDEI CTNAG 7 cut(s) 234, 284, 757, 806, 1202, 1559, 1619
BstF5I GGATG 4 cut(s) 599, 653, 1026, 2039
BstFNI CGCG 4 cut(s) 677, 977, 1074, 1547
BstHHI GCGC 5 cut(s) 979, 1074, 1076, 1549, 1715
BstKTI GATC 8 cut(s) 139, 337, 427, 499, 553, 864, 1327, 1404
BstMAI GTCTC 6 cut(s) 214, 807, 1403, 2078, 2093, 2144
BstMBI GATC 8 cut(s) 136, 334, 424, 496, 550, 861, 1324, 1401
BstMWI GCNNNNNNNGC 7 cut(s) 102, 748, 831, 1080, 1433, 1868, 1956
BstNI CCWGG 2 cut(s) 671, 1269
BstNSI RCATGY 1 cut(s) 281
BstSCI CCNGG 2 cut(s) 669, 1267
BstSFI CTRYAG 2 cut(s) 1038, 1374
BstSLI GKGCMC 1 cut(s) 1352
BstUI CGCG 4 cut(s) 677, 977, 1074, 1547
BstV1I GCAGC 6 cut(s) 36, 1024, 1052, 1093, 1273, 1919
BstXI CCANNNNNNTGG 1 cut(s) 368
Bsu15I ATCGAT 1 cut(s) 183
BsuRI GGCC 4 cut(s) 1160, 1442, 1579, 1655
BsuTUI ATCGAT 1 cut(s) 183
BtsCI GGATG 4 cut(s) 599, 653, 1026, 2039
BtsI GCAGTG 2 cut(s) 796, 1383
BtsIMutI CAGTG 4 cut(s) 796, 1383, 1469, 1697
Cac8I GCNNGC 8 cut(s) 54, 1020, 1074, 1085, 1394, 1425, 1429, 1738
CciI TCATGA 1 cut(s) 127
CfoI GCGC 5 cut(s) 979, 1074, 1076, 1549, 1715
Cfr13I GGNCC 1 cut(s) 1501
ClaI ATCGAT 1 cut(s) 183
CseI GACGC 5 cut(s) 683, 1420, 1703, 2126, 2177
Csp6I GTAC 4 cut(s) 694, 973, 1915, 2107
CviQI GTAC 4 cut(s) 694, 973, 1915, 2107
DdeI CTNAG 7 cut(s) 234, 284, 757, 806, 1202, 1559, 1619
DpnI GATC 8 cut(s) 138, 336, 426, 498, 552, 863, 1326, 1403
DpnII GATC 8 cut(s) 136, 334, 424, 496, 550, 861, 1324, 1401
DraI TTTAAA 1 cut(s) 2221
Eam1104I CTCTTC 1 cut(s) 2147
EarI CTCTTC 1 cut(s) 2147
EciI GGCGGA 1 cut(s) 1826
Ecl136II GAGCTC 6 cut(s) 231, 343, 536, 688, 772, 1360
Eco130I CCWWGG 2 cut(s) 438, 1299
Eco147I AGGCCT 1 cut(s) 1442
Eco24I GRGCYC 6 cut(s) 233, 345, 538, 690, 774, 1362
Eco47I GGWCC 1 cut(s) 1501
Eco53kI GAGCTC 6 cut(s) 231, 343, 536, 688, 772, 1360
Eco57I CTGAAG 2 cut(s) 864, 1014
EcoICRI GAGCTC 6 cut(s) 231, 343, 536, 688, 772, 1360
EcoRII CCWGG 2 cut(s) 669, 1267
EcoT14I CCWWGG 2 cut(s) 438, 1299
EcoT38I GRGCYC 6 cut(s) 233, 345, 538, 690, 774, 1362
ErhI CCWWGG 2 cut(s) 438, 1299
Esp3I CGTCTC 1 cut(s) 2144
FalI AAGNNNNNCTT 2 cut(s) 1939, 1971
FaqI GGGAC 1 cut(s) 405
Fnu4HI GCNGC 8 cut(s) 50, 289, 292, 1038, 1041, 1107, 1262, 1908
FokI GGATG 4 cut(s) 586, 640, 1013, 2026
FriOI GRGCYC 6 cut(s) 233, 345, 538, 690, 774, 1362
Fsp4HI GCNGC 8 cut(s) 50, 289, 292, 1038, 1041, 1107, 1262, 1908
FspBI CTAG 1 cut(s) 1923
GlaI GCGC 5 cut(s) 978, 1073, 1075, 1548, 1714
GluI GCNGC 8 cut(s) 50, 289, 292, 1038, 1041, 1107, 1262, 1908
GsaI CCCAGC 2 cut(s) 313, 392
HaeIII GGCC 4 cut(s) 1160, 1442, 1579, 1655
HapII CCGG 2 cut(s) 274, 903
HgaI GACGC 5 cut(s) 683, 1420, 1703, 2126, 2177
HhaI GCGC 5 cut(s) 979, 1074, 1076, 1549, 1715
Hin6I GCGC 5 cut(s) 977, 1072, 1074, 1547, 1713
HinP1I GCGC 5 cut(s) 977, 1072, 1074, 1547, 1713
HincII GTYRAC 3 cut(s) 63, 879, 1123
HindII GTYRAC 3 cut(s) 63, 879, 1123
HinfI GANTC 7 cut(s) 190, 304, 641, 710, 802, 1272, 1686
HpaII CCGG 2 cut(s) 274, 903
HphI GGTGA 7 cut(s) 260, 625, 1144, 1618, 1651, 1659, 2159
Hpy166II GTNNAC 6 cut(s) 63, 223, 879, 1123, 1543, 1681
Hpy188III TCNNGA 5 cut(s) 128, 338, 554, 799, 1132
Hpy8I GTNNAC 6 cut(s) 63, 223, 879, 1123, 1543, 1681
Hpy99I CGWCG 5 cut(s) 682, 1436, 1697, 2193, 2196
HpyAV CCTTC 8 cut(s) 322, 658, 762, 1042, 1123, 1410, 1747, 1873
HpyCH4III ACNGT 4 cut(s) 114, 134, 208, 1701
HpyCH4IV ACGT 3 cut(s) 505, 692, 1293
HpyF10VI GCNNNNNNNGC 7 cut(s) 102, 748, 831, 1080, 1433, 1868, 1956
HpyF3I CTNAG 7 cut(s) 234, 284, 757, 806, 1202, 1559, 1619
HpySE526I ACGT 3 cut(s) 505, 692, 1293
HspAI GCGC 5 cut(s) 977, 1072, 1074, 1547, 1713
KpnI GGTACC 1 cut(s) 2110
Kzo9I GATC 8 cut(s) 136, 334, 424, 496, 550, 861, 1324, 1401
LmnI GCTCC 5 cut(s) 769, 777, 905, 1015, 1365
Lsp1109I GCAGC 6 cut(s) 36, 1024, 1052, 1093, 1273, 1919
LweI GCATC 1 cut(s) 821
MaeI CTAG 1 cut(s) 1923
MaeII ACGT 3 cut(s) 505, 692, 1293
MaeIII GTNAC 4 cut(s) 80, 1384, 1779, 1840
MalI GATC 8 cut(s) 138, 336, 426, 498, 552, 863, 1326, 1403
MboI GATC 8 cut(s) 136, 334, 424, 496, 550, 861, 1324, 1401
MboII GAAGA 9 cut(s) 329, 344, 389, 1007, 1010, 1082, 1411, 1502, 2134
MfeI CAATTG 1 cut(s) 1505
MhlI GDGCHC 7 cut(s) 233, 345, 538, 690, 774, 1352, 1362
MlyI GAGTC 2 cut(s) 298, 635
MmeI TCCRAC 4 cut(s) 438, 526, 747, 889
MroXI GAANNNNTTC 1 cut(s) 1685
MseI TTAA 7 cut(s) 9, 108, 821, 1898, 2199, 2220, 2225
MslI CAYNNNNRTG 5 cut(s) 87, 132, 185, 1805, 2062
MspA1I CMGCKG 1 cut(s) 288
MspCI CTTAAG 1 cut(s) 1897
MspI CCGG 2 cut(s) 274, 903
MspR9I CCNGG 2 cut(s) 671, 1269
MunI CAATTG 1 cut(s) 1505
Mva1269I GAATGC 2 cut(s) 96, 1226
MvaI CCWGG 2 cut(s) 671, 1269
MvnI CGCG 4 cut(s) 677, 977, 1074, 1547
MwoI GCNNNNNNNGC 7 cut(s) 102, 748, 831, 1080, 1433, 1868, 1956
NdeII GATC 8 cut(s) 136, 334, 424, 496, 550, 861, 1324, 1401
NlaIV GGNNCC 2 cut(s) 2005, 2108
NmuCI GTSAC 3 cut(s) 80, 1384, 1840
NspI RCATGY 1 cut(s) 281
NspV TTCGAA 1 cut(s) 2214
OliI CACNNNNGTG 2 cut(s) 87, 2062
PagI TCATGA 1 cut(s) 127
PauI GCGCGC 1 cut(s) 1072
PceI AGGCCT 1 cut(s) 1442
PcsI WCGNNNNNNNCGW 1 cut(s) 1571
PctI GAATGC 2 cut(s) 96, 1226
PdmI GAANNNNTTC 1 cut(s) 1685
PfeI GAWTC 5 cut(s) 190, 710, 802, 1272, 1686
PfoI TCCNGGA 1 cut(s) 669
PkrI GCNGC 8 cut(s) 51, 290, 293, 1039, 1042, 1108, 1263, 1909
PleI GAGTC 2 cut(s) 298, 635
PpsI GAGTC 2 cut(s) 298, 635
Ppu21I YACGTR 1 cut(s) 693
PshBI ATTAAT 1 cut(s) 2225
PsiI TTATAA 1 cut(s) 2239
Psp124BI GAGCTC 6 cut(s) 233, 345, 538, 690, 774, 1362
Psp1406I AACGTT 1 cut(s) 505
Psp6I CCWGG 2 cut(s) 669, 1267
PspFI CCCAGC 2 cut(s) 309, 388
PspGI CCWGG 2 cut(s) 669, 1267
PspN4I GGNNCC 2 cut(s) 2005, 2108
PspPI GGNCC 1 cut(s) 1501
PstI CTGCAG 2 cut(s) 1042, 1378
PteI GCGCGC 1 cut(s) 1072
RsaI GTAC 4 cut(s) 695, 974, 1916, 2108
RsaNI GTAC 4 cut(s) 694, 973, 1915, 2107
RseI CAYNNNNRTG 5 cut(s) 87, 132, 185, 1805, 2062
SacI GAGCTC 6 cut(s) 233, 345, 538, 690, 774, 1362
SaqAI TTAA 7 cut(s) 9, 108, 821, 1898, 2199, 2220, 2225
SatI GCNGC 8 cut(s) 50, 289, 292, 1038, 1041, 1107, 1262, 1908
Sau3AI GATC 8 cut(s) 136, 334, 424, 496, 550, 861, 1324, 1401
Sau96I GGNCC 1 cut(s) 1501
SchI GAGTC 2 cut(s) 298, 635
ScrFI CCNGG 2 cut(s) 671, 1269
SduI GDGCHC 7 cut(s) 233, 345, 538, 690, 774, 1352, 1362
SfaNI GCATC 1 cut(s) 821
SfcI CTRYAG 2 cut(s) 1038, 1374
SfuI TTCGAA 1 cut(s) 2214
SinI GGWCC 1 cut(s) 1501
SmiI ATTTAAAT 1 cut(s) 2221
SmiMI CAYNNNNRTG 5 cut(s) 87, 132, 185, 1805, 2062
SmlI CTYRAG 4 cut(s) 215, 226, 1730, 1897
SmoI CTYRAG 4 cut(s) 215, 226, 1730, 1897
SseBI AGGCCT 1 cut(s) 1442
SsiI CCGC 9 cut(s) 194, 288, 291, 294, 562, 722, 1081, 1339, 1811
SspMI CTAG 1 cut(s) 1923
SstI GAGCTC 6 cut(s) 233, 345, 538, 690, 774, 1362
StuI AGGCCT 1 cut(s) 1442
StyD4I CCNGG 2 cut(s) 669, 1267
StyI CCWWGG 2 cut(s) 438, 1299
SwaI ATTTAAAT 1 cut(s) 2221
TaaI ACNGT 4 cut(s) 114, 134, 208, 1701
TaiI ACGT 3 cut(s) 508, 695, 1296
TauI GCSGC 2 cut(s) 291, 294
TfiI GAWTC 5 cut(s) 190, 710, 802, 1272, 1686
Tru1I TTAA 7 cut(s) 9, 108, 821, 1898, 2199, 2220, 2225
Tru9I TTAA 7 cut(s) 9, 108, 821, 1898, 2199, 2220, 2225
TscAI CASTG 4 cut(s) 796, 1383, 1476, 1704
TseFI GTSAC 3 cut(s) 80, 1384, 1840
TseI GCWGC 6 cut(s) 49, 1037, 1040, 1106, 1261, 1907
Tsp45I GTSAC 3 cut(s) 80, 1384, 1840
TspRI CASTG 4 cut(s) 796, 1383, 1476, 1704
Vha464I CTTAAG 1 cut(s) 1897
VpaK11BI GGWCC 1 cut(s) 1501
VspI ATTAAT 1 cut(s) 2225
XapI RAATTY 3 cut(s) 251, 1100, 2216
XceI RCATGY 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 1685
XspI CTAG 1 cut(s) 1923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.