pycom03g23360

Bromodomain and WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003305
Physical Location & Seq
Forward (+)
23207695 .. 23213859
6165 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2061 bp
ATGGATCCTCGGAAGTGTACTTTGTCTGGTGGTGCAACTTCTCTTGCTATGGCACCTCCAAGCATTTTGAATATGTTAAATGAGAAGGCTCAGTTTGAGAAAGAAGAGAGGGATACAGGTCATGTTGTCGAGGCTGGAGTCGATATAGACATTAGAGAGGTTTATTTTTTGATTATGCATTTTCTGTCTCTTGGGCCATGCCAGAGGACTTTCGAACAGTTTGGGAATGATCTTCTGGAACATCAGCTTCTACCTAGAAGGTTTCATGCTTGGTTTTCAAGGAGCAGAATAGGAAGTGACAATAGTAATGAGGATGCTACCTCTTTCCCATTGAGTTACAACAACTTGGTTGAAAGGTATCCTCATATAGAGAGAGATCACTTGGTAAAGCTTCTAAGGCAACTGCTACTGAGTATAGCCACTCCTTTACATGGCAAGGTTGGAAGAAGTACTCTGAATGCAGCTGATGTCCCTACATTACTGGGAACTGGTTCCTTCTCGCTTCTGGATAGTGATAGGAATAAGGAAAATAAGAGAGTTAAGCCCCTGCCAGCTCACCTGCGTTGGCCTTACATGCAGGCTGATCAAATTCATGGGCTTAGTTTAAGGGAAATTGGAGGAGGTTTCACAAAGCATCATCGAGCTCCATCCATTCGCTCTGCGTGTTATGCGATTGCTAAACCATCAACTATGGTGCAGAAGATGAACAACAAAAAGAAGCTACGGGGGCACCGTAATGCTGTCTACTGTGCCATTTTTGATCGGTCCGGGAGATATGTTATCACGGGTGCAGATGATCGGCTTGTGAAGATATGGTCAATGGAAACTGCACTTTGCTTGGCTAGCTGTCGTGGACATGAAGGAGACATTACTGACTTGGCTGTAAGTTCGAACAATGCTTTGGTGGCATCTGCTTCAAATGACTTTTCCATTCGAGTTTGGCGTTTGCCAGATGGATTTCCAATTTCAGTTCTTCAGGGGCATACTGGGGCTGTGACTGCTATTGCTTTTAGTCCCAGGCTTAGTGCTGTTTACCAACTTCTATCGTCATCAGATGATGGGACTTGTCGAATCTGGGATGCTAGGTCCTCCCAATGCCCGCTGCGAATATACATGCCAAAACCTTCAGAGACTTCAACAGGGAAGGGCAATGCTTTTGCAATCACTGGATCGTCCTCGAGCAATGGTCCACAGAGCCATCAAATACTTTGTTGTGCTTACAATGCCAATGGGACTGTTTTTGTCACTGGTAGCTCTGACACTTTTGCAAGGGTCTGGAATGCTTTAAAATCTAATACAGATAACTCAGAACAACCAATACATGAGATGGATGTACTGGCGGGCCACGAGAATGACGTGAATTATGTCCAATTCAGTGGTTGTGTCATTCCTTCAAAGTCTTCATTTTCTGACTCGGTCAAGGAGGAGAATAATGGGAAGTTCAAAAATTCCTGGTTTTGTCACAACAACATAGTTACCTGCTCTCGTGATGGCAGTGCCATTATATGGGTTCCAAGATCACACAAATTCCATGGGAAAGTTGGACGTTGGACACGTGCATATCATCTAAAAGTTCCACCTCCCCCACTACCTCCTCAGCCTCCTCGAGGAGGCCCACGTCAAAGATTTCTTCCCACCCCTCGTGGTGTTAACATGATTGTGTGGAGCCTGGATAACCGCTTTGTGCTTGCAGCTATCATGGATTGCAGAATTTGTGTTTGGAATGCTGTTGATGGTAGCTTAGTACATTCTTTAACCGGTCACACTGCATCGTCTTATGTTTTGGATGTTCATCCCTTCAATCCTCGAATTGCTATGAGTGCTGGGTATGATGGGCAAACAATAGTTTGGGATATATGGGAGGGCGTGCCTATTAAGATATACGAACTTGGACATGTAAAGTTGGTTGATGGGAAGTTTTCGGCGGACGGTACATCAATAGTACTTTCAGATGACGTTGGCCAAATATATTTAATAAACACAGGTGAAGGCGAGTCTCAAAAAGATGCTGAATATCGATCAGTTCTTCCTTGGGGATTATCGCCCCCTTGCACGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007010 GO:0007088 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007346 GO:0008104 GO:0008150 GO:0008284 GO:0008286 GO:0008360 GO:0009719 GO:0009725 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010564 GO:0010604 GO:0010628 GO:0010638 GO:0010646 GO:0010647 GO:0010648 GO:0010799 GO:0010800 GO:0010941 GO:0015031 GO:0015833 GO:0016043 GO:0017038 GO:0019219 GO:0019220 GO:0019222 GO:0022603 GO:0022604 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033043 GO:0033135 GO:0033138 GO:0033365 GO:0034504 GO:0034613 GO:0040008 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042393 GO:0042886 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043434 GO:0043567 GO:0043568 GO:0044424 GO:0044464 GO:0045184 GO:0045787 GO:0045840 GO:0045893 GO:0045931 GO:0045935 GO:0045937 GO:0045944 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051716 GO:0051726 GO:0051781 GO:0051783 GO:0051785 GO:0060255 GO:0060548 GO:0065007 GO:0065008 GO:0070577 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0090068 GO:0140030 GO:0140033 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141 GO:2001233 GO:2001234 GO:2001236 GO:2001237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_resp Bromodomain WD40

Protein Analysis

687

Amino Acids

75.81

Weight (kDa)

8.58

Isoelectric Point (pI)

38.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRWD1_N PF25437 42 - 138 1.1e-31 BRWD1-like, N-terminal domain
Beta-prop_THOC3 PF25174 232 - 319 7.5e-18 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 232 - 368 3e-28 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 232 - 366 1.4e-15 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 233 - 319 2.4e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 233 - 367 1.7e-24 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 235 - 372 1.9e-10 CAF1B/HIR1 beta-propeller domain
WD40_Gbeta PF25391 236 - 366 6.2e-15 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 236 - 316 4e-11 Prp19 WD40 domain
WD40 PF00400 238 - 273 3.6e-07 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 238 - 315 1.9e-11 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 241 - 365 5.8e-12 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 244 - 359 6.9e-08 MABP1/WDR62 second WD40 domain
Beta-prop_SCAP PF24017 257 - 364 6e-10 SCAP Beta-propeller
Beta-prop_TEP1_2nd PF25047 261 - 370 5e-11 TEP-1 second beta-propeller
WD40 PF00400 279 - 314 8.7e-08 WD domain, G-beta repeat
EIF3I PF24805 284 - 366 7.2e-07 EIF3I
Beta-prop_THOC3 PF25174 286 - 369 1.1e-12 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 289 - 369 3.3e-13 WDHD1 first WD40 domain
WD40_Prp19 PF24814 309 - 374 2.1e-06 Prp19 WD40 domain
WD40 PF00400 321 - 360 2.1e-08 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 547 - 665 2.4e-11 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 549 - 626 6.6e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 550 - 672 7.8e-13 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 567
AbsI CCTCGAGG 1 cut(s) 1605
Acc36I ACCTGC 2 cut(s) 567, 1487
AccB1I GGYRCC 2 cut(s) 52, 729
AccB7I CCANNNNNTGG 1 cut(s) 1506
AccI GTMKAC 1 cut(s) 744
AciI CCGC 4 cut(s) 1100, 1340, 1678, 1925
AclWI GGATC 2 cut(s) 12, 1177
AcoI YGGCCR 1 cut(s) 1960
AcsI RAATTY 4 cut(s) 588, 1447, 1526, 1710
AcuI CTGAAG 2 cut(s) 959, 1110
AcvI CACGTG 1 cut(s) 1556
AfaI GTAC 6 cut(s) 19, 451, 1335, 1746, 1933, 1944
AfiI CCNNNNNNNGG 6 cut(s) 431, 1506, 1607, 1610, 2053, 2054
AflIII ACRYGT 2 cut(s) 1553, 1894
AgeI ACCGGT 1 cut(s) 1757
AgsI TTSAA 8 cut(s) 70, 279, 353, 918, 1137, 1395, 1444, 1801
AjiI CACGTC 2 cut(s) 1357, 1619
AjnI CCWGG 3 cut(s) 1016, 1451, 1668
AjuI GAANNNNNNNTTGG 4 cut(s) 884, 916, 1702, 1734
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 4 cut(s) 192, 858, 1124, 2001
AlwI GGATC 2 cut(s) 12, 1177
Ama87I CYCGRG 2 cut(s) 1177, 1605
AoxI GGCC 5 cut(s) 194, 566, 1342, 1612, 1960
ApeKI GCWGC 3 cut(s) 461, 1102, 1691
ApoI RAATTY 4 cut(s) 588, 1447, 1526, 1710
AsiGI ACCGGT 1 cut(s) 1757
Asp700I GAANNNNTTC 1 cut(s) 490
AspS9I GGNCC 6 cut(s) 194, 765, 1086, 1187, 1342, 1613
AsuC2I CCSGG 1 cut(s) 769
AsuHPI GGTGA 2 cut(s) 548, 1997
AsuII TTCGAA 2 cut(s) 213, 890
AsuNHI GCTAGC 1 cut(s) 842
AvaI CYCGRG 2 cut(s) 1177, 1605
AvaII GGWCC 3 cut(s) 765, 1086, 1187
BaeGI GKGCMC 1 cut(s) 732
BalI TGGCCA 1 cut(s) 1962
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 52, 729
BanII GRGCYC 1 cut(s) 646
BauI CACGAG 3 cut(s) 1346, 1485, 1641
BbrPI CACGTG 1 cut(s) 1556
BbsI GAAGAC 1 cut(s) 1392
Bbv12I GWGCWC 1 cut(s) 646
BbvCI CCTCAGC 1 cut(s) 1596
BbvI GCAGC 3 cut(s) 473, 1089, 1703
BciT130I CCWGG 3 cut(s) 1018, 1453, 1670
BciVI GTATCC 2 cut(s) 106, 369
BclI TGATCA 1 cut(s) 583
BcnI CCSGG 1 cut(s) 769
BcoDI GTCTC 4 cut(s) 192, 858, 1124, 2001
BfaI CTAG 3 cut(s) 255, 843, 1083
BfuAI ACCTGC 2 cut(s) 567, 1487
BfuI GTATCC 2 cut(s) 106, 369
BisI GCNGC 3 cut(s) 462, 1103, 1692
BlsI GCNGC 3 cut(s) 463, 1104, 1693
BmcAI AGTACT 2 cut(s) 451, 1944
Bme1390I CCNGG 4 cut(s) 769, 1018, 1453, 1670
Bme18I GGWCC 3 cut(s) 765, 1086, 1187
BmeT110I CYCGRG 2 cut(s) 1177, 1605
BmgBI CACGTC 2 cut(s) 1357, 1619
BmgT120I GGNCC 6 cut(s) 194, 765, 1086, 1187, 1342, 1613
BmiI GGNNCC 6 cut(s) 6, 54, 493, 731, 1512, 1667
BmrFI CCNGG 4 cut(s) 769, 1018, 1453, 1670
BmrI ACTGGG 2 cut(s) 491, 996
BmsI GCATC 6 cut(s) 304, 643, 917, 1069, 1778, 1996
BmtI GCTAGC 1 cut(s) 846
BmuI ACTGGG 2 cut(s) 491, 996
BpiI GAAGAC 1 cut(s) 1392
BpmI CTGGAG 1 cut(s) 156
Bpu10I CCTNAGC 1 cut(s) 1596
Bpu14I TTCGAA 2 cut(s) 213, 890
BpuMI CCSGG 1 cut(s) 769
Bsa29I ATCGAT 1 cut(s) 2017
BsaAI YACGTR 1 cut(s) 1556
BsaBI GATNNNNATC 1 cut(s) 1791
BsaJI CCNNGG 4 cut(s) 8, 1016, 1531, 2030
BsaWI WCCGGW 1 cut(s) 1757
Bsc4I CCNNNNNNNGG 6 cut(s) 431, 1506, 1607, 1610, 2053, 2054
Bse118I RCCGGY 1 cut(s) 1757
Bse1I ACTGG 6 cut(s) 486, 493, 991, 1171, 1252, 1341
Bse3DI GCAATG 2 cut(s) 1156, 1189
Bse8I GATNNNNATC 1 cut(s) 1791
BseBI CCWGG 3 cut(s) 1018, 1453, 1670
BseCI ATCGAT 1 cut(s) 2017
BseDI CCNNGG 4 cut(s) 8, 1016, 1531, 2030
BseGI GGATG 6 cut(s) 319, 647, 1084, 1336, 1792, 1792
BseJI GATNNNNATC 1 cut(s) 1791
BseLI CCNNNNNNNGG 6 cut(s) 431, 1506, 1607, 1610, 2053, 2054
BseMI GCAATG 2 cut(s) 1156, 1189
BseMII CTCAG 4 cut(s) 104, 401, 1320, 1610
BseNI ACTGG 6 cut(s) 486, 493, 991, 1171, 1252, 1341
BseRI GAGGAG 5 cut(s) 633, 1439, 1584, 1593, 1623
BseSI GKGCMC 1 cut(s) 732
BseXI GCAGC 3 cut(s) 473, 1089, 1703
BseYI CCCAGC 1 cut(s) 1823
BsgI GTGCAG 3 cut(s) 716, 810, 813
BshFI GGCC 5 cut(s) 196, 568, 1344, 1614, 1962
BshNI GGYRCC 2 cut(s) 52, 729
BshTI ACCGGT 1 cut(s) 1757
BshVI ATCGAT 1 cut(s) 2017
BsiHKAI GWGCWC 1 cut(s) 646
BsiHKCI CYCGRG 2 cut(s) 1177, 1605
BsiSI CCGG 2 cut(s) 768, 1758
BslFI GGGAC 4 cut(s) 455, 999, 1075, 1246
BslI CCNNNNNNNGG 6 cut(s) 431, 1506, 1607, 1610, 2053, 2054
BsmAI GTCTC 4 cut(s) 192, 858, 1124, 2001
BsmFI GGGAC 4 cut(s) 455, 999, 1075, 1246
BsmI GAATGC 3 cut(s) 463, 1285, 1729
BsnI GGCC 5 cut(s) 196, 568, 1344, 1614, 1962
BsoBI CYCGRG 2 cut(s) 1177, 1605
Bsp119I TTCGAA 2 cut(s) 213, 890
Bsp1286I GDGCHC 2 cut(s) 646, 732
Bsp143I GATC 9 cut(s) 4, 229, 376, 583, 760, 796, 1169, 1517, 2018
Bsp19I CCATGG 1 cut(s) 1531
BspACI CCGC 4 cut(s) 1100, 1340, 1678, 1925
BspANI GGCC 5 cut(s) 196, 568, 1344, 1614, 1962
BspCNI CTCAG 4 cut(s) 103, 402, 1319, 1609
BspDI ATCGAT 1 cut(s) 2017
BspLI GGNNCC 6 cut(s) 6, 54, 493, 731, 1512, 1667
BspMI ACCTGC 2 cut(s) 567, 1487
BspOI GCTAGC 1 cut(s) 846
BspPI GGATC 2 cut(s) 12, 1177
BspT104I TTCGAA 2 cut(s) 213, 890
BspT107I GGYRCC 2 cut(s) 52, 729
BsrDI GCAATG 2 cut(s) 1156, 1189
BsrFI RCCGGY 1 cut(s) 1757
BsrI ACTGG 6 cut(s) 486, 493, 991, 1171, 1252, 1341
BssAI RCCGGY 1 cut(s) 1757
BssECI CCNNGG 4 cut(s) 8, 1016, 1531, 2030
BssMI GATC 9 cut(s) 4, 229, 376, 583, 760, 796, 1169, 1517, 2018
BssSI CACGAG 3 cut(s) 1346, 1485, 1641
BssT1I CCWWGG 2 cut(s) 1531, 2030
Bst2BI CACGAG 3 cut(s) 1346, 1485, 1641
Bst2UI CCWGG 3 cut(s) 1018, 1453, 1670
Bst4CI ACNGT 5 cut(s) 219, 734, 749, 1237, 1931
Bst6I CTCTTC 1 cut(s) 99
BstBAI YACGTR 1 cut(s) 1556
BstBI TTCGAA 2 cut(s) 213, 890
BstC8I GCNNGC 7 cut(s) 552, 579, 844, 1100, 1342, 1689, 1868
BstDEI CTNAG 8 cut(s) 90, 395, 410, 599, 1022, 1306, 1596, 1741
BstDSI CCRYGG 1 cut(s) 1531
BstENI CCTNNNNNAGG 2 cut(s) 1605, 1608
BstF5I GGATG 6 cut(s) 319, 647, 1084, 1336, 1792, 1792
BstKTI GATC 9 cut(s) 7, 232, 379, 586, 763, 799, 1172, 1520, 2021
BstMAI GTCTC 4 cut(s) 192, 858, 1124, 2001
BstMBI GATC 9 cut(s) 4, 229, 376, 583, 760, 796, 1169, 1517, 2018
BstMWI GCNNNNNNNGC 9 cut(s) 397, 574, 668, 727, 843, 905, 998, 1223, 1820
BstNI CCWGG 3 cut(s) 1018, 1453, 1670
BstNSI RCATGY 3 cut(s) 577, 1117, 1898
BstSCI CCNGG 4 cut(s) 767, 1016, 1451, 1668
BstSLI GKGCMC 1 cut(s) 732
BstV1I GCAGC 3 cut(s) 473, 1089, 1703
BstV2I GAAGAC 1 cut(s) 1392
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 1376
BstYI RGATCY 1 cut(s) 4
Bsu15I ATCGAT 1 cut(s) 2017
BsuI GTATCC 2 cut(s) 106, 369
BsuRI GGCC 5 cut(s) 196, 568, 1344, 1614, 1962
BsuTUI ATCGAT 1 cut(s) 2017
BtgI CCRYGG 1 cut(s) 1531
BtrI CACGTC 2 cut(s) 1357, 1619
BtsCI GGATG 6 cut(s) 319, 647, 1084, 1336, 1792, 1792
BtsI GCAGTG 2 cut(s) 1501, 1764
BtsIMutI CAGTG 5 cut(s) 1164, 1245, 1381, 1501, 1764
BveI ACCTGC 2 cut(s) 567, 1487
Cac8I GCNNGC 7 cut(s) 552, 579, 844, 1100, 1342, 1689, 1868
Cfr10I RCCGGY 1 cut(s) 1757
Cfr13I GGNCC 6 cut(s) 194, 765, 1086, 1187, 1342, 1613
ClaI ATCGAT 1 cut(s) 2017
CpoI CGGWCCG 1 cut(s) 765
Csp6I GTAC 6 cut(s) 18, 450, 1334, 1745, 1932, 1943
CspAI ACCGGT 1 cut(s) 1757
CspI CGGWCCG 1 cut(s) 765
CviQI GTAC 6 cut(s) 18, 450, 1334, 1745, 1932, 1943
DdeI CTNAG 8 cut(s) 90, 395, 410, 599, 1022, 1306, 1596, 1741
DpnI GATC 9 cut(s) 6, 231, 378, 585, 762, 798, 1171, 1519, 2020
DpnII GATC 9 cut(s) 4, 229, 376, 583, 760, 796, 1169, 1517, 2018
DraI TTTAAA 1 cut(s) 1287
EaeI YGGCCR 1 cut(s) 1960
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
EciI GGCGGA 1 cut(s) 1940
Ecl136II GAGCTC 1 cut(s) 644
Eco130I CCWWGG 2 cut(s) 1531, 2030
Eco24I GRGCYC 1 cut(s) 646
Eco47I GGWCC 3 cut(s) 765, 1086, 1187
Eco53kI GAGCTC 1 cut(s) 644
Eco57I CTGAAG 2 cut(s) 959, 1110
Eco72I CACGTG 1 cut(s) 1556
Eco88I CYCGRG 2 cut(s) 1177, 1605
EcoICRI GAGCTC 1 cut(s) 644
EcoNI CCTNNNNNAGG 2 cut(s) 1605, 1608
EcoO109I RGGNCCY 1 cut(s) 1086
EcoRII CCWGG 3 cut(s) 1016, 1451, 1668
EcoT14I CCWWGG 2 cut(s) 1531, 2030
EcoT22I ATGCAT 1 cut(s) 180
EcoT38I GRGCYC 1 cut(s) 646
ErhI CCWWGG 2 cut(s) 1531, 2030
FaqI GGGAC 4 cut(s) 455, 999, 1075, 1246
FauI CCCGC 2 cut(s) 1107, 1333
FbaI TGATCA 1 cut(s) 583
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 3 cut(s) 462, 1103, 1692
FokI GGATG 6 cut(s) 326, 634, 1091, 1343, 1779, 1799
FriOI GRGCYC 1 cut(s) 646
Fsp4HI GCNGC 3 cut(s) 462, 1103, 1692
FspBI CTAG 3 cut(s) 255, 843, 1083
GluI GCNGC 3 cut(s) 462, 1103, 1692
GsaI CCCAGC 1 cut(s) 1827
GsuI CTGGAG 1 cut(s) 156
HaeIII GGCC 5 cut(s) 196, 568, 1344, 1614, 1962
HapII CCGG 2 cut(s) 768, 1758
HincII GTYRAC 1 cut(s) 1651
HindII GTYRAC 1 cut(s) 1651
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 4 cut(s) 138, 1071, 1412, 1994
HpaI GTTAAC 1 cut(s) 1651
HpaII CCGG 2 cut(s) 768, 1758
HphI GGTGA 2 cut(s) 548, 1997
Hpy166II GTNNAC 6 cut(s) 18, 745, 854, 1033, 1190, 1651
Hpy188I TCNGA 8 cut(s) 12, 456, 1054, 1129, 1258, 1309, 1411, 1951
Hpy188III TCNNGA 4 cut(s) 236, 506, 1276, 1487
Hpy8I GTNNAC 6 cut(s) 18, 745, 854, 1033, 1190, 1651
HpyAV CCTTC 9 cut(s) 79, 252, 505, 854, 1134, 1138, 1401, 1807, 1982
HpyCH4III ACNGT 5 cut(s) 219, 734, 749, 1237, 1931
HpyCH4IV ACGT 5 cut(s) 1356, 1546, 1555, 1618, 1956
HpyF10VI GCNNNNNNNGC 9 cut(s) 397, 574, 668, 727, 843, 905, 998, 1223, 1820
HpyF3I CTNAG 8 cut(s) 90, 395, 410, 599, 1022, 1306, 1596, 1741
HpySE526I ACGT 5 cut(s) 1356, 1546, 1555, 1618, 1956
Ksp22I TGATCA 1 cut(s) 583
KspAI GTTAAC 1 cut(s) 1651
Kzo9I GATC 9 cut(s) 4, 229, 376, 583, 760, 796, 1169, 1517, 2018
LmnI GCTCC 3 cut(s) 282, 649, 1665
Lsp1109I GCAGC 3 cut(s) 473, 1089, 1703
LweI GCATC 6 cut(s) 304, 643, 917, 1069, 1778, 1996
MaeI CTAG 3 cut(s) 255, 843, 1083
MaeII ACGT 5 cut(s) 1356, 1546, 1555, 1618, 1956
MaeIII GTNAC 7 cut(s) 296, 335, 994, 1243, 1460, 1474, 1760
MalI GATC 9 cut(s) 6, 231, 378, 585, 762, 798, 1171, 1519, 2020
MboI GATC 9 cut(s) 4, 229, 376, 583, 760, 796, 1169, 1517, 2018
MboII GAAGA 9 cut(s) 116, 224, 456, 712, 820, 965, 1392, 1622, 2018
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 646, 732
MlsI TGGCCA 1 cut(s) 1962
MluCI AATT 9 cut(s) 588, 612, 963, 1360, 1370, 1447, 1526, 1710, 1809
MluNI TGGCCA 1 cut(s) 1962
MlyI GAGTC 3 cut(s) 147, 1406, 2003
MmeI TCCRAC 3 cut(s) 421, 1522, 1529
Mox20I TGGCCA 1 cut(s) 1962
Mph1103I ATGCAT 1 cut(s) 180
MroXI GAANNNNTTC 1 cut(s) 490
MscI TGGCCA 1 cut(s) 1962
MseI TTAA 8 cut(s) 77, 540, 605, 1286, 1650, 1754, 1875, 1973
MslI CAYNNNNRTG 3 cut(s) 735, 1350, 1658
Msp20I TGGCCA 1 cut(s) 1962
MspA1I CMGCKG 2 cut(s) 464, 1102
MspI CCGG 2 cut(s) 768, 1758
MspR9I CCNGG 4 cut(s) 769, 1018, 1453, 1670
Mva1269I GAATGC 3 cut(s) 463, 1285, 1729
MvaI CCWGG 3 cut(s) 1018, 1453, 1670
MwoI GCNNNNNNNGC 9 cut(s) 397, 574, 668, 727, 843, 905, 998, 1223, 1820
NciI CCSGG 1 cut(s) 769
NcoI CCATGG 1 cut(s) 1531
NdeII GATC 9 cut(s) 4, 229, 376, 583, 760, 796, 1169, 1517, 2018
NheI GCTAGC 1 cut(s) 842
NlaIV GGNNCC 6 cut(s) 6, 54, 493, 731, 1512, 1667
NmuCI GTSAC 5 cut(s) 296, 994, 1243, 1460, 1760
NsiI ATGCAT 1 cut(s) 180
NspI RCATGY 3 cut(s) 577, 1117, 1898
NspV TTCGAA 2 cut(s) 213, 890
PaeR7I CTCGAG 2 cut(s) 1177, 1605
PaqCI CACCTGC 1 cut(s) 567
PciI ACATGT 1 cut(s) 1894
PcsI WCGNNNNNNNCGW 4 cut(s) 730, 940, 1353, 1552
PctI GAATGC 3 cut(s) 463, 1285, 1729
PdmI GAANNNNTTC 1 cut(s) 490
PfeI GAWTC 1 cut(s) 1071
PflFI GACNNNGTC 1 cut(s) 1415
PflMI CCANNNNNTGG 1 cut(s) 1506
PfoI TCCNGGA 1 cut(s) 767
PinAI ACCGGT 1 cut(s) 1757
PkrI GCNGC 3 cut(s) 463, 1104, 1693
PleI GAGTC 3 cut(s) 146, 1406, 2002
PmaCI CACGTG 1 cut(s) 1556
PmlI CACGTG 1 cut(s) 1556
PpsI GAGTC 3 cut(s) 146, 1406, 2002
Ppu21I YACGTR 1 cut(s) 1556
PpuMI RGGWCCY 1 cut(s) 1086
PscI ACATGT 1 cut(s) 1894
Psp124BI GAGCTC 1 cut(s) 646
Psp5II RGGWCCY 1 cut(s) 1086
Psp6I CCWGG 3 cut(s) 1016, 1451, 1668
PspCI CACGTG 1 cut(s) 1556
PspFI CCCAGC 1 cut(s) 1823
PspGI CCWGG 3 cut(s) 1016, 1451, 1668
PspN4I GGNNCC 6 cut(s) 6, 54, 493, 731, 1512, 1667
PspPI GGNCC 6 cut(s) 194, 765, 1086, 1187, 1342, 1613
PspPPI RGGWCCY 1 cut(s) 1086
PspXI VCTCGAGB 2 cut(s) 1177, 1605
PsrI GAACNNNNNNTAC 2 cut(s) 1302, 1334
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 1415
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 6 cut(s) 19, 451, 1335, 1746, 1933, 1944
RsaNI GTAC 6 cut(s) 18, 450, 1334, 1745, 1932, 1943
RseI CAYNNNNRTG 3 cut(s) 735, 1350, 1658
Rsr2I CGGWCCG 1 cut(s) 765
RsrII CGGWCCG 1 cut(s) 765
SacI GAGCTC 1 cut(s) 646
SaqAI TTAA 8 cut(s) 77, 540, 605, 1286, 1650, 1754, 1875, 1973
SatI GCNGC 3 cut(s) 462, 1103, 1692
Sau3AI GATC 9 cut(s) 4, 229, 376, 583, 760, 796, 1169, 1517, 2018
Sau96I GGNCC 6 cut(s) 194, 765, 1086, 1187, 1342, 1613
ScaI AGTACT 2 cut(s) 451, 1944
SchI GAGTC 3 cut(s) 147, 1406, 2003
ScrFI CCNGG 4 cut(s) 769, 1018, 1453, 1670
SduI GDGCHC 2 cut(s) 646, 732
SfaNI GCATC 6 cut(s) 304, 643, 917, 1069, 1778, 1996
Sfr274I CTCGAG 2 cut(s) 1177, 1605
SfuI TTCGAA 2 cut(s) 213, 890
SinI GGWCC 3 cut(s) 765, 1086, 1187
SlaI CTCGAG 2 cut(s) 1177, 1605
SmiMI CAYNNNNRTG 3 cut(s) 735, 1350, 1658
SmlI CTYRAG 2 cut(s) 1177, 1605
SmoI CTYRAG 2 cut(s) 1177, 1605
Sse9I AATT 9 cut(s) 588, 612, 963, 1360, 1370, 1447, 1526, 1710, 1809
SsiI CCGC 4 cut(s) 1100, 1340, 1678, 1925
SspMI CTAG 3 cut(s) 255, 843, 1083
SstI GAGCTC 1 cut(s) 646
StyD4I CCNGG 4 cut(s) 767, 1016, 1451, 1668
StyI CCWWGG 2 cut(s) 1531, 2030
TaaI ACNGT 5 cut(s) 219, 734, 749, 1237, 1931
TaiI ACGT 5 cut(s) 1359, 1549, 1558, 1621, 1959
TaqII GACCGA 2 cut(s) 753, 1405
TasI AATT 9 cut(s) 588, 612, 963, 1360, 1370, 1447, 1526, 1710, 1809
TatI WGTACW 5 cut(s) 17, 449, 1333, 1744, 1942
TfiI GAWTC 1 cut(s) 1071
Tru1I TTAA 8 cut(s) 77, 540, 605, 1286, 1650, 1754, 1875, 1973
Tru9I TTAA 8 cut(s) 77, 540, 605, 1286, 1650, 1754, 1875, 1973
TscAI CASTG 5 cut(s) 1171, 1252, 1381, 1501, 1771
TseFI GTSAC 5 cut(s) 296, 994, 1243, 1460, 1760
TseI GCWGC 3 cut(s) 461, 1102, 1691
Tsp45I GTSAC 5 cut(s) 296, 994, 1243, 1460, 1760
TspDTI ATGAA 6 cut(s) 254, 581, 719, 873, 1392, 1781
TspRI CASTG 5 cut(s) 1171, 1252, 1381, 1501, 1771
Tth111I GACNNNGTC 1 cut(s) 1415
Van91I CCANNNNNTGG 1 cut(s) 1506
VpaK11BI GGWCC 3 cut(s) 765, 1086, 1187
XagI CCTNNNNNAGG 2 cut(s) 1605, 1608
XapI RAATTY 4 cut(s) 588, 1447, 1526, 1710
XceI RCATGY 3 cut(s) 577, 1117, 1898
XcmI CCANNNNNNNNNTGG 1 cut(s) 1538
XhoI CTCGAG 2 cut(s) 1177, 1605
XmiI GTMKAC 1 cut(s) 744
XmnI GAANNNNTTC 1 cut(s) 490
XspI CTAG 3 cut(s) 255, 843, 1083
ZrmI AGTACT 2 cut(s) 451, 1944
Zsp2I ATGCAT 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.