Rroxscaffold_3G00218900

Bromodomain and WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003305
Physical Location & Seq
Reverse (-)
1103815 .. 1116675
12861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00218900.1

Sequence Viewer

Length: 4977 bp
ATGGATCCTTGGAAGTGCACTCCGTTTGGTGGTGCAAATTCTCTTAATATGGCACCTTCAAATATATTAAGTAAGGTGAATGAGAGGGCTAAATTTGAAAAAGAAGAGAGGGATACAGGTCACACTGTTGAGGCTGGAGTAGATATAGATATTAGAGAAGTATACTTTCTGATTATGCACTTTCTATCTGTTGGGCCATGTCGGAGGACTTTTGAACAATTTGCAAATGATCTTGTGGAACATGAGCTCCTGCCTAGAAGGTATCATGCTTGGTTTTCAAGAAGCAGTATAAGCAGTAGCAATGATAATGATAATAACACCTCTTTCCCACTGAGTTACACCAAATTGGTTGAAAGGTATCCTCATATAGAGAAGGATCACTTGGTAAAGCTTCTAAAGCAACTGATGTTGAGTATAGCTACTCCTTTGCATGGCAAGGTTGGAAGAAATACTCTGAATGCAGCTGATGTCCCTACATTATTGGGAACTGGTTCTTTCTCGCTTCTGGATTGTGATAGGAATGTGGTGAATAGGCGAGTTAAGCCGCTTCCTGCTTACCTGCGTTGGCCTTACATGCAGGCTGGTCAAGTTCATGGACTTGGTTTAAGGGAAATTGGAGGAGGTTTTGCTAAGCATCATCGTGCACCATCTATTCGCTCTGCATGCTATGCCATTGCCAAACCATCAACTATGGTGCAAAAGATGAACAACAAAAAGAAGCTAAGGGGGCACCGTAATGCTGTCTATTGTGGTTTTTGGTTGCCTTACAACATGCATCCTCCTGGTGCTTCAATGGCTACTCGGTATATTAACCCTCGAGCTTCTATGCTTTATCTTGTAGCCATTTTTGATCGGTCGGGGAGATATGTTATCACTGGATCGGATGATCGTCTTGTCAAGATTTGGTCAATGGAAACTGCACTTTGCTTGGCTAGCTGTCGTGGACATGAAGGTGACATTACTGACTTGGCTGTGAGTTCAAACAACACTTTGGTGGCATCTGCATCAAATGATTTTGTTATTCGAGTTTGGCGTTTGCCAGATGGATATCCCATTTCCGTTCTGCAGGGCCATACTGGAGCTGTGACTGCTATAGCATTTAGTCCAAGGCTTAGTGCAATTTACCAACTTTTATCGTCATCAGATGACGGGACTTGTCGAATTTGGGATGCTAGGTCCTCTCAGTGCCCTCCACGAATATTCATGCCAAAACCTTCGGACCCTTTAACAGGGAAGAGCAATGGCATTTCAAGTCTTGGACCCTCATCAAGCAGTGGCCCACAGGGCCATCAAATACTTTGTTGTGCTTACAATGCTAGTGGAACTGTTTTTGTCACTGGTAGCTCTGACACTTTTGCCCGGGTATGGAGTGCTGTGAAATCTAATGCAGATGACTCAGAACAACCAATACATGAGATGGATGTATTGTCTGGCCATGAGAATGATGTCAATTATGTGCAATTCAGTGGTTGTGCCATTCCATCAAAATCTTCATTTTCTGACTCTTTGAAGGAGGAGAATATTATGAAGTCCAAAAATTCCTGGTTTTGTCACAATAACATCGTTACCTGCTCTCGCGATGGCAGTGCCATTATATGGGTTCCAAAATCACACAGATCACATGGGAAATTTGGACGATGGATTCGTGCTTACCATCTCAAAGTTCCACCTCCCCCACTGCCTCCTCAACCTCCTCGAGGTGGCCCACGTCAGAGATTTCTTCCTACCCCTCGTGGTGTTAACATGATTGTATGGAGCCTGGATAACCGCTTTGTGCTTGCAGCTATCATGGATTGCAGAATCTGCGTTTGGAATGCTGTTGATGGTAGCTTAGTACATTCTTTGACCGGTCATAATGCATCTGTAAGTGGGTATCTGATATTCATAACAGATCAGTTTTCATTTGATTCTGAATATCTTAATCTATTGCTCTTGTGGCTCACTTGTCTGCGGTCAAATTCTTGGCAGTCTTATGTTTTGGATGTTCATCCCTTTAATCCTCGGATTGCTATGAGTGCTGGGTATGATGGACAAACAATCATATGGGATATCTGGGAAGGAACACCCATCAAGATTTATGAAGTTGGACACGTTAAGTTGGTTGATGGGAAGTTTTCTGCGGATGGGACATCGATTGTACTTTCAGATGATGTGGGCCAAATATATTTAATAAACACAGGTGAAGGAGAGTCCCAGAAAGATGCTAAATATGATCAGTTCTTTCTAGGAGATTATCGCCCCCTTGTGCGGGATACTGCTGGACATCTTCTTGATCAGGAGACACAACTTCCTGCTTATCGAAGGAATCTTCAAGATCCTCTCTGCGATTCAAGTATGATTCCATATCCAGAGCCATACCAGAGTCAATACCAGCGGCGGCGACTCGGTGCATTGGGAATGGAGTGGAAACCTTCATCAGTAAATTTTGCAGTTGGCCTGGATATTGGTGCAGGCATGGACTATCATTTGCCTCCGTTGCCCGATTGGGAGAGAGTGATAGAGCCACTTCCAGACTTCATCGATGCCTTGCTATGGGAGCCAGAAAATGAATTCATAAGTGAAGATACTGATTCAGATTTTAATGTCACTGAGGAAAATTCTAGTGAAGATGAGAAAGGAAATATTAGCACCAGCTCTTCTAGTGCTCCAGAATGTAGTGCAGAAGACAGTGAAGTTGGATGCAGCAATCAGGATGGCCTTCGCAGATCAAGAAGAAAAACACAAAAGGTTACCTCTTCTGAGAGACGTGGGAAGAAAAGGAATTTGGGTGAGGAGGATGGCATTATATCTGGGAGTAATAGAATTAAAAATCCAAAAGGTGGCCGAAAAGTTTCAAAGAGGAACTGTAGAGCAAAAACGTCAAGACCCCAGAGAGTTGCAGCACGGAATGCTCGAAATGTGCTTTCTCAGAATCCGGGAACATCTACAGATGGAGAAGAAGATGACTGGGAAGCTGATTCATCAGATAGTGAGCCACAGCAGAAAAAGTTCCGTAGTCAAAGCAATGAGTGTGATGGAAGCTTCCAGAATATGCAAGAGTTACAGAATAATAAAAAACCATCTCTTCGTGAGATTCATGATATAGCCAATCCTCTTGCTGTTCCTGAGTCTCAAACAAATGTTACAAGAAAAAAATTGGTTTTGAAGCTTCCGCTCCGTCGTGATTTGAAGAAGCAAGAAGCTCTGCAGGATTCTTCATCTTCTAGATATCGCGAAGTCGGTCAAGATAAGAAGATTAATAATAGCTCTGTGGATGGAGCTTCATCTTTGGCAGATGCACTTGATTTGCCGTTCTCTGGAAATTTCATGAGCAATGTATTTACAGATCCTGGGCAAACCGTAGAGGCTGGGTTTCCTTTGGAGGCATCTTCATGTAACATGGAGAACAAAATTAGGTGGGGAGAGGTCAAGACACGGACATCAAAGCATGCAAGATCAGGAGATCCCATACCAATTGATCCAGCCGCTGGGTCGCTTGTCAGTTCTGAAGTCCATATGGCAGAGAGAAATGTCGTTCGGGATAAGGAACATATTAGAGAGTTTTCTCATGAATCAACGTCAAAAGGTTCTGGAATAAAGCACTCTGATGAATCAATGGATAATGCTTCCCTTAAGTTTCTTGCACAAGAAACAGGTTCTGGGAACTTAATGCCTGAAGATCCTTCATGCATGGACCGGAATTTAAATCTGGAAGTACCAGAAATGGGTGGAGGTTCTGGCACATTGAGTACGTGGAGATTTTTCTGCAAGGATAGGACAAAAACAGAAGGGTTTGATGGTAAATTGGAAGAAAATACTTCTCGTATCAATGACCATTATGATGCAGGAATGAATCTCCCTGAAGCTGCAACTGATTCAGCACGCAGGCTAAGAACCTTAAAGATCAAGGCGACTTCACGGGAACCAGACTCTGTGAGCCGTATCCCTAAGTCGAGAGGGGGCCAGCAAAGGACATCTATGGATGCAGAAGATCCCCCTGCCAAGGTCCGTGATACAAGACTTCAGAGATCAAGGTCCACTAGAAATCAGTCTGACCAAAGTTTGTCAACCCAAAGGATGTTGGATAACCCTGTTGGAAAACTTTCATGGCTGATGTTGTCTAAACACGAGGAAGGTTACCGTTATATTCCTCAGCTAGGTGATGAAGTTGTATACTTGAGACAGGGTCATCAAGAGTATACGGAATTGGCTTTGAAGTCAGATCAAGGCCCTTGGGGTCCGTGGGGATCAATGAAGGAAAAAATCAGAGCTGTGGAAATCTGCAAGGTTGTAGGCCTCGAGTATGACTCCATGCCTGGTTCAGGGGAAAGCTGTAGCAAGATCTTATTCAGATTTGTGGAGTCTTCTTCTGCCGTGTGTGGTAAAACCTTCAAATTAACATTGCCTGAAATTGACTTTAATGACTTCATAGTTGAGAAAAGCTGGTACGATGCCGCCATTGGAAGAAACTGGGCAGCGGGAGAACACTGTGAGGTCTGGTGGAGGGACTCAGATGGAGGCGGAGCGTGGTGGCAAGGCCGAATTGTGCACTGTCAGGCAAAATCTCATGAGTTTCCTGATAGCCCGTGGTTAAGATACGCGGTTAGGTATGAGAATGAAGATGATATACCTCATAGGCATTGTCCTTGGGAACTGCATGATGTATCCATGTCGTGGGAAGCACCGCATATTGATCATGAAAGCAGAGATAAGCTGCTTCACTATTTTTCTAAATCGGAGAAGAAGGACTTGGAGGCCATCCAACAAATGAATCAAGCTGTTCTGAAGGTGGATTTCTGCAACAGCTTTCCTGTTCAATTGTACCCTGAACTAATCAAAGCAAGGTTAAAGAATGACTATTATAGGAGCTTGGAAGCAGTGAAGCATGACATAACCCAGATGCTAGAAAACGCCCGGCATTACTTCAAGAGAAATAACTTGCAAGCCAGGATGAAGCACCTTTCAAAATGGTTTAAGAAAAAAATTTCTAGGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007010 GO:0007088 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007346 GO:0008104 GO:0008150 GO:0008284 GO:0008286 GO:0008360 GO:0009719 GO:0009725 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010564 GO:0010604 GO:0010628 GO:0010638 GO:0010646 GO:0010647 GO:0010648 GO:0010799 GO:0010800 GO:0010941 GO:0015031 GO:0015833 GO:0016043 GO:0017038 GO:0019219 GO:0019220 GO:0019222 GO:0022603 GO:0022604 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033043 GO:0033135 GO:0033138 GO:0033365 GO:0034504 GO:0034613 GO:0040008 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042393 GO:0042886 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043434 GO:0043567 GO:0043568 GO:0044424 GO:0044464 GO:0045184 GO:0045787 GO:0045840 GO:0045893 GO:0045931 GO:0045935 GO:0045937 GO:0045944 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051716 GO:0051726 GO:0051781 GO:0051783 GO:0051785 GO:0060255 GO:0060548 GO:0065007 GO:0065008 GO:0070577 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0090068 GO:0140030 GO:0140033 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141 GO:2001233 GO:2001234 GO:2001236 GO:2001237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_resp Bromodomain WD40

Protein Analysis

1658

Amino Acids

186.52

Weight (kDa)

7.28

Isoelectric Point (pI)

47.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRWD1_N PF25437 41 - 138 1.2e-31 BRWD1-like, N-terminal domain
WD40_Prp19 PF24814 281 - 396 5.6e-16 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 282 - 370 1.6e-16 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 283 - 399 3.4e-25 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 283 - 394 3.5e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 283 - 396 5.7e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 284 - 397 1e-20 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 284 - 395 3.4e-10 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 284 - 389 9.1e-07 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 286 - 407 8.9e-16 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 287 - 397 3.2e-10 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 287 - 391 6.4e-11 WDR3 second beta-propeller domain
Beta-prop_SCAP PF24017 287 - 393 8.8e-10 SCAP Beta-propeller
WD40_Gbeta PF25391 288 - 395 1.6e-12 G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1 PF24105 305 - 400 1e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 309 - 344 3.7e-07 WD domain, G-beta repeat
EIF3I PF24805 314 - 396 2.9e-06 EIF3I
Beta-prop_THOC3 PF25174 316 - 398 1.2e-12 THOC3 beta-propeller domain
WD40 PF00400 350 - 390 4.1e-08 WD domain, G-beta repeat
BRWD_AD PF25313 1373 - 1549 8.9e-57 BRWD/PHIP ancillary domain-like domain
Bromodomain PF00439 1603 - 1639 9.4e-06 Bromodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1695
Acc36I ACCTGC 2 cut(s) 567, 1577
AccB1I GGYRCC 2 cut(s) 52, 729
AccB7I CCANNNNNTGG 4 cut(s) 1596, 2819, 3467, 4653
AccBSI CCGCTC 1 cut(s) 3154
AccI GTMKAC 3 cut(s) 162, 4152, 4178
AccII CGCG 3 cut(s) 1578, 3213, 4580
AcoI YGGCCR 2 cut(s) 1432, 2821
AcuI CTGAAG 5 cut(s) 3507, 3675, 3861, 3986, 4784
AfaI GTAC 6 cut(s) 1836, 2139, 3696, 3730, 4427, 4802
AflII CTTAAG 1 cut(s) 3611
AflIII ACRYGT 1 cut(s) 2089
AgeI ACCGGT 1 cut(s) 1847
AjiI CACGTC 2 cut(s) 1709, 2747
AjnI CCWGG 7 cut(s) 781, 1541, 1758, 2436, 3328, 4294, 4925
AjuI GAANNNNNNNTTGG 8 cut(s) 365, 397, 1792, 1824, 2747, 2779, 4712, 4744
AleI CACNNNNGTG 1 cut(s) 992
AloI GAACNNNNNNTCC 2 cut(s) 231, 263
Alw21I GWGCWC 5 cut(s) 20, 249, 646, 2647, 4530
Alw26I GTCTC 4 cut(s) 2273, 2737, 3114, 4153
Alw44I GTGCAC 3 cut(s) 16, 642, 4526
AlwNI CAGNNNCTG 5 cut(s) 1803, 3329, 3467, 3638, 3911
Ama87I CYCGRG 4 cut(s) 816, 1359, 1695, 4277
ApaLI GTGCAC 3 cut(s) 16, 642, 4526
ApeKI GCWGC 7 cut(s) 461, 1781, 2682, 2879, 3845, 4454, 4693
ArsI GACNNNNNNTTYG 2 cut(s) 1626, 1658
AseI ATTAAT 1 cut(s) 3237
AsiGI ACCGGT 1 cut(s) 1847
Asp700I GAANNNNTTC 4 cut(s) 490, 2830, 2987, 4081
AsuC2I CCSGG 4 cut(s) 1360, 1361, 2916, 4894
AsuHPI GGTGA 6 cut(s) 88, 538, 965, 2192, 2780, 4151
AsuNHI GCTAGC 1 cut(s) 932
AvaI CYCGRG 4 cut(s) 816, 1359, 1695, 4277
AvaII GGWCC 7 cut(s) 1176, 1219, 1259, 3673, 3985, 4014, 4217
BaeGI GKGCMC 5 cut(s) 20, 646, 732, 1190, 4530
BalI TGGCCA 1 cut(s) 1434
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 52, 729
BanII GRGCYC 1 cut(s) 249
BarI GAAGNNNNNNTAC 2 cut(s) 4590, 4622
BauI CACGAG 2 cut(s) 1731, 4106
BbsI GAAGAC 2 cut(s) 2670, 4335
Bbv12I GWGCWC 5 cut(s) 20, 249, 646, 2647, 4530
BbvCI CCTCAGC 1 cut(s) 4131
BbvI GCAGC 7 cut(s) 473, 1793, 2694, 2891, 3832, 4466, 4680
BceAI ACGGC 3 cut(s) 3274, 3903, 4337
BcgI CGANNNNNNTGC 2 cut(s) 1568, 1602
BciT130I CCWGG 7 cut(s) 783, 1543, 1760, 2438, 3330, 4296, 4927
BciVI GTATCC 5 cut(s) 106, 369, 2245, 3932, 4654
BclI TGATCA 3 cut(s) 2212, 2272, 4672
BcnI CCSGG 4 cut(s) 1360, 1361, 2916, 4894
BcoDI GTCTC 4 cut(s) 2273, 2737, 3114, 4153
BfmI CTRYAG 6 cut(s) 1064, 1092, 2845, 2925, 3185, 4312
BfrI CTTAAG 1 cut(s) 3611
BfuAI ACCTGC 2 cut(s) 567, 1577
BfuI GTATCC 5 cut(s) 106, 369, 2245, 3932, 4654
BglI GCCNNNNNGGC 1 cut(s) 1284
BglII AGATCT 1 cut(s) 4320
BlpI GCTNAGC 1 cut(s) 630
Bme18I GGWCC 7 cut(s) 1176, 1219, 1259, 3673, 3985, 4014, 4217
BmeT110I CYCGRG 4 cut(s) 816, 1359, 1695, 4277
BmgBI CACGTC 2 cut(s) 1709, 2747
BmrI ACTGGG 2 cut(s) 2956, 4459
BmtI GCTAGC 1 cut(s) 936
BmuI ACTGGG 2 cut(s) 2956, 4459
BpiI GAAGAC 2 cut(s) 2670, 4335
BplI GAGNNNNNCTC 2 cut(s) 4271, 4303
BpmI CTGGAG 3 cut(s) 156, 1098, 2631
Bpu10I CCTNAGC 2 cut(s) 722, 4131
Bpu1102I GCTNAGC 1 cut(s) 630
BpuEI CTTGAG 1 cut(s) 4177
BpuMI CCSGG 4 cut(s) 1360, 1361, 2916, 4894
Bsa29I ATCGAT 2 cut(s) 2132, 2520
BsaAI YACGTR 1 cut(s) 3732
BsaBI GATNNNNATC 2 cut(s) 1047, 1986
BsaWI WCCGGW 2 cut(s) 1847, 3675
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bse118I RCCGGY 1 cut(s) 1847
Bse1I ACTGG 6 cut(s) 493, 880, 1081, 1342, 2951, 4454
Bse3DI GCAATG 6 cut(s) 307, 672, 1246, 3010, 3319, 4379
Bse8I GATNNNNATC 2 cut(s) 1047, 1986
BseBI CCWGG 7 cut(s) 783, 1543, 1760, 2438, 3330, 4296, 4927
BseCI ATCGAT 2 cut(s) 2132, 2520
BseJI GATNNNNATC 2 cut(s) 1047, 1986
BseMI GCAATG 6 cut(s) 307, 672, 1246, 3010, 3319, 4379
BseMII CTCAG 9 cut(s) 323, 1196, 1410, 2580, 2730, 2921, 3096, 4145, 4503
BseNI ACTGG 6 cut(s) 493, 880, 1081, 1342, 2951, 4454
BseRI GAGGAG 5 cut(s) 633, 1529, 1674, 1683, 2786
BseSI GKGCMC 5 cut(s) 20, 646, 732, 1190, 4530
BseXI GCAGC 7 cut(s) 473, 1793, 2694, 2891, 3832, 4466, 4680
BseYI CCCAGC 3 cut(s) 2018, 3347, 3467
BsgI GTGCAG 3 cut(s) 903, 2469, 2679
Bsh1236I CGCG 3 cut(s) 1578, 3213, 4580
Bsh1285I CGRYCG 1 cut(s) 857
BshNI GGYRCC 2 cut(s) 52, 729
BshTI ACCGGT 1 cut(s) 1847
BshVI ATCGAT 2 cut(s) 2132, 2520
BsiEI CGRYCG 1 cut(s) 857
BsiHKAI GWGCWC 5 cut(s) 20, 249, 646, 2647, 4530
BsiHKCI CYCGRG 4 cut(s) 816, 1359, 1695, 4277
BsiSI CCGG 5 cut(s) 1360, 1848, 2915, 3676, 4894
BslFI GGGAC 5 cut(s) 455, 1165, 2140, 2176, 4499
BsmAI GTCTC 4 cut(s) 2273, 2737, 3114, 4153
BsmBI CGTCTC 1 cut(s) 2737
BsmFI GGGAC 5 cut(s) 455, 1165, 2140, 2176, 4499
BsmI GAATGC 3 cut(s) 463, 1819, 2893
BsoBI CYCGRG 4 cut(s) 816, 1359, 1695, 4277
Bsp1286I GDGCHC 7 cut(s) 20, 249, 646, 732, 1190, 2647, 4530
Bsp1720I GCTNAGC 1 cut(s) 630
Bsp68I TCGCGA 2 cut(s) 1578, 3213
BspCNI CTCAG 9 cut(s) 324, 1195, 1409, 2581, 2731, 2920, 3097, 4144, 4502
BspDI ATCGAT 2 cut(s) 2132, 2520
BspFNI CGCG 3 cut(s) 1578, 3213, 4580
BspHI TCATGA 5 cut(s) 3076, 3306, 3547, 4546, 4675
BspMAI CTGCAG 2 cut(s) 1068, 3189
BspMI ACCTGC 2 cut(s) 567, 1577
BspOI GCTAGC 1 cut(s) 936
BspQI GCTCTTC 2 cut(s) 1229, 2641
BspT107I GGYRCC 2 cut(s) 52, 729
BspTI CTTAAG 1 cut(s) 3611
BsrBI CCGCTC 1 cut(s) 3154
BsrDI GCAATG 6 cut(s) 307, 672, 1246, 3010, 3319, 4379
BsrFI RCCGGY 1 cut(s) 1847
BsrI ACTGG 6 cut(s) 493, 880, 1081, 1342, 2951, 4454
BssAI RCCGGY 1 cut(s) 1847
BssNAI GTATAC 3 cut(s) 163, 4153, 4179
BssSI CACGAG 2 cut(s) 1731, 4106
BssT1I CCWWGG 5 cut(s) 8, 1106, 3981, 4211, 4625
Bst1107I GTATAC 3 cut(s) 163, 4153, 4179
Bst2BI CACGAG 2 cut(s) 1731, 4106
Bst2UI CCWGG 7 cut(s) 783, 1543, 1760, 2438, 3330, 4296, 4927
Bst4CI ACNGT 9 cut(s) 127, 734, 1327, 2669, 2846, 3340, 4121, 4469, 4532
Bst6I CTCTTC 5 cut(s) 99, 1229, 2641, 2740, 3069
BstAFI CTTAAG 1 cut(s) 3611
BstAPI GCANNNNNTGC 3 cut(s) 668, 1803, 2888
BstBAI YACGTR 1 cut(s) 3732
BstDSI CCRYGG 2 cut(s) 4220, 4565
BstEII GGTNACC 2 cut(s) 2728, 4115
BstENI CCTNNNNNAGG 4 cut(s) 1227, 1695, 4134, 4299
BstFNI CGCG 3 cut(s) 1578, 3213, 4580
BstMAI GTCTC 4 cut(s) 2273, 2737, 3114, 4153
BstMCI CGRYCG 1 cut(s) 857
BstNI CCWGG 7 cut(s) 783, 1543, 1760, 2438, 3330, 4296, 4927
BstNSI RCATGY 4 cut(s) 577, 666, 775, 3431
BstPI GGTNACC 2 cut(s) 2728, 4115
BstSFI CTRYAG 6 cut(s) 1064, 1092, 2845, 2925, 3185, 4312
BstSLI GKGCMC 5 cut(s) 20, 646, 732, 1190, 4530
BstUI CGCG 3 cut(s) 1578, 3213, 4580
BstV1I GCAGC 7 cut(s) 473, 1793, 2694, 2891, 3832, 4466, 4680
BstV2I GAAGAC 2 cut(s) 2670, 4335
BstX2I RGATCY 7 cut(s) 4, 2314, 3325, 3442, 3658, 3970, 4320
BstYI RGATCY 7 cut(s) 4, 2314, 3325, 3442, 3658, 3970, 4320
BstZ17I GTATAC 3 cut(s) 163, 4153, 4179
Bsu15I ATCGAT 2 cut(s) 2132, 2520
BsuI GTATCC 5 cut(s) 106, 369, 2245, 3932, 4654
BsuTUI ATCGAT 2 cut(s) 2132, 2520
BtgI CCRYGG 2 cut(s) 4220, 4565
BtgZI GCGATG 1 cut(s) 1593
BtrI CACGTC 2 cut(s) 1709, 2747
BtsI GCAGTG 4 cut(s) 1279, 1591, 1676, 4863
BtuMI TCGCGA 2 cut(s) 1578, 3213
BveI ACCTGC 2 cut(s) 567, 1577
CaiI CAGNNNCTG 5 cut(s) 1803, 3329, 3467, 3638, 3911
CciI TCATGA 5 cut(s) 3076, 3306, 3547, 4546, 4675
Cfr10I RCCGGY 1 cut(s) 1847
Cfr9I CCCGGG 1 cut(s) 1359
ClaI ATCGAT 2 cut(s) 2132, 2520
Csp6I GTAC 6 cut(s) 1835, 2138, 3695, 3729, 4426, 4801
CspAI ACCGGT 1 cut(s) 1847
CspCI CAANNNNNGTGG 2 cut(s) 1300, 1335
CviQI GTAC 6 cut(s) 1835, 2138, 3695, 3729, 4426, 4801
DraI TTTAAA 1 cut(s) 3684
EaeI YGGCCR 2 cut(s) 1432, 2821
Eam1104I CTCTTC 5 cut(s) 99, 1229, 2641, 2740, 3069
EarI CTCTTC 5 cut(s) 99, 1229, 2641, 2740, 3069
EciI GGCGGA 1 cut(s) 4515
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 5 cut(s) 8, 1106, 3981, 4211, 4625
Eco147I AGGCCT 1 cut(s) 4275
Eco24I GRGCYC 1 cut(s) 249
Eco32I GATATC 3 cut(s) 1049, 2050, 3209
Eco47I GGWCC 7 cut(s) 1176, 1219, 1259, 3673, 3985, 4014, 4217
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 5 cut(s) 3507, 3675, 3861, 3986, 4784
Eco88I CYCGRG 4 cut(s) 816, 1359, 1695, 4277
Eco91I GGTNACC 2 cut(s) 2728, 4115
EcoICRI GAGCTC 1 cut(s) 247
EcoNI CCTNNNNNAGG 4 cut(s) 1227, 1695, 4134, 4299
EcoO109I RGGNCCY 2 cut(s) 1176, 4208
EcoO65I GGTNACC 2 cut(s) 2728, 4115
EcoRI GAATTC 1 cut(s) 2549
EcoRII CCWGG 7 cut(s) 781, 1541, 1758, 2436, 3328, 4294, 4925
EcoRV GATATC 3 cut(s) 1049, 2050, 3209
EcoT14I CCWWGG 5 cut(s) 8, 1106, 3981, 4211, 4625
EcoT22I ATGCAT 3 cut(s) 777, 1861, 3671
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 5 cut(s) 8, 1106, 3981, 4211, 4625
Esp3I CGTCTC 1 cut(s) 2737
FalI AAGNNNNNCTT 4 cut(s) 365, 397, 4712, 4744
FaqI GGGAC 5 cut(s) 455, 1165, 2140, 2176, 4499
FauI CCCGC 2 cut(s) 2241, 4450
FauNDI CATATG 2 cut(s) 2042, 3495
FbaI TGATCA 3 cut(s) 2212, 2272, 4672
FblI GTMKAC 3 cut(s) 162, 4152, 4178
FriOI GRGCYC 1 cut(s) 249
GsaI CCCAGC 3 cut(s) 2022, 3351, 3471
GsuI CTGGAG 3 cut(s) 156, 1098, 2631
HapII CCGG 5 cut(s) 1360, 1848, 2915, 3676, 4894
HincII GTYRAC 2 cut(s) 1741, 4047
HindII GTYRAC 2 cut(s) 1741, 4047
HindIII AAGCTT 3 cut(s) 389, 3019, 3146
HpaI GTTAAC 1 cut(s) 1741
HpaII CCGG 5 cut(s) 1360, 1848, 2915, 3676, 4894
HphI GGTGA 6 cut(s) 88, 538, 965, 2192, 2780, 4151
Hpy99I CGWCG 1 cut(s) 3162
HpyCH4III ACNGT 9 cut(s) 127, 734, 1327, 2669, 2846, 3340, 4121, 4469, 4532
HpyCH4IV ACGT 6 cut(s) 1708, 2091, 2746, 2858, 3557, 3731
HpySE526I ACGT 6 cut(s) 1708, 2091, 2746, 2858, 3557, 3731
Ksp22I TGATCA 3 cut(s) 2212, 2272, 4672
KspAI GTTAAC 1 cut(s) 1741
LguI GCTCTTC 2 cut(s) 1229, 2641
LmnI GCTCC 9 cut(s) 252, 1079, 1755, 2536, 2650, 3159, 3257, 4502, 4845
Lsp1109I GCAGC 7 cut(s) 473, 1793, 2694, 2891, 3832, 4466, 4680
MaeII ACGT 6 cut(s) 1708, 2091, 2746, 2858, 3557, 3731
MbiI CCGCTC 1 cut(s) 3154
MfeI CAATTG 2 cut(s) 3453, 4796
MflI RGATCY 7 cut(s) 4, 2314, 3325, 3442, 3658, 3970, 4320
MhlI GDGCHC 7 cut(s) 20, 249, 646, 732, 1190, 2647, 4530
MlsI TGGCCA 1 cut(s) 1434
MluNI TGGCCA 1 cut(s) 1434
MmeI TCCRAC 7 cut(s) 182, 421, 2065, 2656, 4041, 4054, 4765
Mox20I TGGCCA 1 cut(s) 1434
Mph1103I ATGCAT 3 cut(s) 777, 1861, 3671
MroXI GAANNNNTTC 4 cut(s) 490, 2830, 2987, 4081
MscI TGGCCA 1 cut(s) 1434
MslI CAYNNNNRTG 8 cut(s) 639, 735, 951, 992, 1440, 3370, 3585, 3819
Msp20I TGGCCA 1 cut(s) 1434
MspA1I CMGCKG 4 cut(s) 464, 2374, 3467, 4457
MspCI CTTAAG 1 cut(s) 3611
MspI CCGG 5 cut(s) 1360, 1848, 2915, 3676, 4894
MunI CAATTG 2 cut(s) 3453, 4796
Mva1269I GAATGC 3 cut(s) 463, 1819, 2893
MvaI CCWGG 7 cut(s) 783, 1543, 1760, 2438, 3330, 4296, 4927
MvnI CGCG 3 cut(s) 1578, 3213, 4580
NciI CCSGG 4 cut(s) 1360, 1361, 2916, 4894
NdeI CATATG 2 cut(s) 2042, 3495
NheI GCTAGC 1 cut(s) 932
NmuCI GTSAC 6 cut(s) 119, 953, 1084, 1333, 1550, 2584
NruI TCGCGA 2 cut(s) 1578, 3213
NsiI ATGCAT 3 cut(s) 777, 1861, 3671
NspI RCATGY 4 cut(s) 577, 666, 775, 3431
OliI CACNNNNGTG 1 cut(s) 992
PaeI GCATGC 2 cut(s) 666, 3431
PaeR7I CTCGAG 3 cut(s) 816, 1695, 4277
PagI TCATGA 5 cut(s) 3076, 3306, 3547, 4546, 4675
PceI AGGCCT 1 cut(s) 4275
PciSI GCTCTTC 2 cut(s) 1229, 2641
PcsI WCGNNNNNNNCGW 3 cut(s) 1030, 1642, 2890
PctI GAATGC 3 cut(s) 463, 1819, 2893
PdmI GAANNNNTTC 4 cut(s) 490, 2830, 2987, 4081
PflFI GACNNNGTC 1 cut(s) 4164
PflMI CCANNNNNTGG 4 cut(s) 1596, 2819, 3467, 4653
PfoI TCCNGGA 1 cut(s) 2914
PinAI ACCGGT 1 cut(s) 1847
Ppu21I YACGTR 1 cut(s) 3732
PpuMI RGGWCCY 1 cut(s) 1176
PshBI ATTAAT 1 cut(s) 3237
Psp124BI GAGCTC 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 1176
Psp6I CCWGG 7 cut(s) 781, 1541, 1758, 2436, 3328, 4294, 4925
PspEI GGTNACC 2 cut(s) 2728, 4115
PspFI CCCAGC 3 cut(s) 2018, 3347, 3467
PspGI CCWGG 7 cut(s) 781, 1541, 1758, 2436, 3328, 4294, 4925
PspPPI RGGWCCY 1 cut(s) 1176
PspXI VCTCGAGB 3 cut(s) 816, 1695, 4277
PsrI GAACNNNNNNTAC 2 cut(s) 1392, 1424
PstI CTGCAG 2 cut(s) 1068, 3189
PstNI CAGNNNCTG 5 cut(s) 1803, 3329, 3467, 3638, 3911
PsuI RGATCY 7 cut(s) 4, 2314, 3325, 3442, 3658, 3970, 4320
PsyI GACNNNGTC 1 cut(s) 4164
PvuII CAGCTG 1 cut(s) 464
RruI TCGCGA 2 cut(s) 1578, 3213
RsaI GTAC 6 cut(s) 1836, 2139, 3696, 3730, 4427, 4802
RsaNI GTAC 6 cut(s) 1835, 2138, 3695, 3729, 4426, 4801
RseI CAYNNNNRTG 8 cut(s) 639, 735, 951, 992, 1440, 3370, 3585, 3819
SacI GAGCTC 1 cut(s) 249
SapI GCTCTTC 2 cut(s) 1229, 2641
SduI GDGCHC 7 cut(s) 20, 249, 646, 732, 1190, 2647, 4530
SfcI CTRYAG 6 cut(s) 1064, 1092, 2845, 2925, 3185, 4312
SfiI GGCCNNNNNGGCC 1 cut(s) 1284
Sfr274I CTCGAG 3 cut(s) 816, 1695, 4277
SinI GGWCC 7 cut(s) 1176, 1219, 1259, 3673, 3985, 4014, 4217
SlaI CTCGAG 3 cut(s) 816, 1695, 4277
SmaI CCCGGG 1 cut(s) 1361
SmiI ATTTAAAT 1 cut(s) 3684
SmiMI CAYNNNNRTG 8 cut(s) 639, 735, 951, 992, 1440, 3370, 3585, 3819
SmlI CTYRAG 5 cut(s) 816, 1695, 3611, 4156, 4277
SmoI CTYRAG 5 cut(s) 816, 1695, 3611, 4156, 4277
SphI GCATGC 2 cut(s) 666, 3431
SseBI AGGCCT 1 cut(s) 4275
SspI AATATT 3 cut(s) 1200, 1522, 2623
SstI GAGCTC 1 cut(s) 249
StuI AGGCCT 1 cut(s) 4275
StyI CCWWGG 5 cut(s) 8, 1106, 3981, 4211, 4625
SwaI ATTTAAAT 1 cut(s) 3684
TaaI ACNGT 9 cut(s) 127, 734, 1327, 2669, 2846, 3340, 4121, 4469, 4532
TaiI ACGT 6 cut(s) 1711, 2094, 2749, 2861, 3560, 3734
TaqII GACCGA 2 cut(s) 843, 3209
TatI WGTACW 2 cut(s) 1834, 2137
TauI GCSGC 5 cut(s) 547, 2377, 2380, 3467, 4436
TseFI GTSAC 6 cut(s) 119, 953, 1084, 1333, 1550, 2584
TseI GCWGC 7 cut(s) 461, 1781, 2682, 2879, 3845, 4454, 4693
Tsp45I GTSAC 6 cut(s) 119, 953, 1084, 1333, 1550, 2584
TspMI CCCGGG 1 cut(s) 1359
Tth111I GACNNNGTC 1 cut(s) 4164
Van91I CCANNNNNTGG 4 cut(s) 1596, 2819, 3467, 4653
Vha464I CTTAAG 1 cut(s) 3611
VneI GTGCAC 3 cut(s) 16, 642, 4526
VpaK11BI GGWCC 7 cut(s) 1176, 1219, 1259, 3673, 3985, 4014, 4217
VspI ATTAAT 1 cut(s) 3237
XagI CCTNNNNNAGG 4 cut(s) 1227, 1695, 4134, 4299
XbaI TCTAGA 1 cut(s) 3203
XceI RCATGY 4 cut(s) 577, 666, 775, 3431
XhoI CTCGAG 3 cut(s) 816, 1695, 4277
XmaI CCCGGG 1 cut(s) 1359
XmiI GTMKAC 3 cut(s) 162, 4152, 4178
XmnI GAANNNNTTC 4 cut(s) 490, 2830, 2987, 4081
Zsp2I ATGCAT 3 cut(s) 777, 1861, 3671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.