pycom04g00250

YT521-B-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005296
Physical Location & Seq
Forward (+)
187951 .. 190430
2480 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1185 bp
ATGCATGGATATGGATTGGTTTCAAGTTCCTTTGGTGGACGCTATCCTCCTGGTAGTTCTTATCAGAGCTCAAACTCTGGTGGTGCTTCATATTCATTTGGTAATGACCAGAACCGACCAAATGTTGACAAGGCCAGAAGAAGAGAGAGGGACTGGGATTCAGTTTCCACTTATAATAATTCACATGATATCTTCAATGACCGCAATCACGGACCAAGGGCTTCGAAGATGAAGGGAAAGAGTACTTCCGACCAGAGTTCTTCATCTAGTGTTAGGAAAATGGATCTATCTGCTTCTGGAATTAACCTTGATTCATTGAACCGGCTAGATTTTGTCACAGAATATGAGGATGCAAAATTCTTTATCATTAAGTCTTTCAGTGAGGACAATGTTCACAAAAGTATTAAATACAATGTTTGGGCTAGCACGCCTCATGGAAACAAGAAACTTGATGCTGCTTATCATGAGGCAAAGAAAAAAGGCAGCTGTGCAGTCTTCCTATTCTTTTCGGTTAATGCTAGCGGACAGTTTTGTGGAGTAGCGGAAATGGTGGGACCTGTAGATTTTGAGAAGGATGCTGATTACTGGCAGCAAGATAGGTGGAGTGGGCAATTTCCGGTTCAGTGGCATATTGTAAAGGATGTTCCTAACATTCGGTTCCGTCACATTCTACTTGAAAATAATGATAATAAGCCTGTCACGCACAGTCGAGATTGTCAGGAGGTGAATCTCAAACAAGGTATTGAGCTGTTGAAAATTTTCAATGATTTTGATGCACGAACATCTATTATAGATGATTTTGAATTTTATGATGAGCGGGAAAAATCCTTGAAGGAAAGGAAAGTTAAACAAGAAGCTAGTTCAACTACAGATGCTTCTGATTCAGTTGCCGTTGATTCTGTTAATGAAATTTCCAATAGTTTTGATCAAGCCCTGGAGTTGAAAGGAAGTAGTAGCAAAGTAGTAGCAGAAACTGAACATGACATTAGCTCAAAAACAGATGCTGCGTCAGTTATACTTGAACATGTTTCCTCCGTGGACCAAATATCTGATAACTTATCTCAAGTATTGCGGTTGGAAGAGGGTGACAAAGAATTAGCTCTGTCATCTGAAAGTAGTAAAGGTGTAGACGACAGCAAGTCAATTGACAGGAAGGCAACAGTAGTATCAACTACTAGTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YTH

Protein Analysis

395

Amino Acids

43.9

Weight (kDa)

5.46

Isoelectric Point (pI)

42.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YTH PF04146 118 - 255 2.2e-51 YT521-B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 174
AccBSI CCGCTC 1 cut(s) 817
AccI GTMKAC 1 cut(s) 1128
AciI CCGC 5 cut(s) 202, 522, 542, 817, 1072
AclWI GGATC 1 cut(s) 291
AcsI RAATTY 4 cut(s) 356, 756, 803, 909
AfaI GTAC 1 cut(s) 244
AflIII ACRYGT 1 cut(s) 1024
AhdI GACNNNNNGTC 1 cut(s) 1138
AhlI ACTAGT 1 cut(s) 1175
AjnI CCWGG 2 cut(s) 49, 933
AluBI AGCT 6 cut(s) 69, 486, 748, 857, 990, 1100
AluI AGCT 6 cut(s) 69, 486, 748, 857, 990, 1100
Alw21I GWGCWC 1 cut(s) 71
AlwI GGATC 1 cut(s) 291
AlwNI CAGNNNCTG 2 cut(s) 974, 1004
AoxI GGCC 1 cut(s) 132
ApeKI GCWGC 4 cut(s) 455, 483, 589, 1004
ApoI RAATTY 4 cut(s) 356, 756, 803, 909
Asp700I GAANNNNTTC 1 cut(s) 758
AspS9I GGNCC 3 cut(s) 212, 554, 1039
AsuHPI GGTGA 2 cut(s) 736, 1097
AsuII TTCGAA 1 cut(s) 224
AsuNHI GCTAGC 2 cut(s) 422, 518
AvaII GGWCC 3 cut(s) 212, 554, 1039
BaeI ACNNNNGTAYC 2 cut(s) 1149, 1182
BanII GRGCYC 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 487
Bbv12I GWGCWC 1 cut(s) 71
BbvI GCAGC 4 cut(s) 442, 495, 601, 991
BceAI ACGGC 1 cut(s) 875
BciT130I CCWGG 2 cut(s) 51, 935
BclI TGATCA 1 cut(s) 925
BcuI ACTAGT 1 cut(s) 1175
BfaI CTAG 6 cut(s) 267, 326, 423, 519, 858, 1176
BfmI CTRYAG 2 cut(s) 558, 867
BisI GCNGC 4 cut(s) 456, 484, 590, 1005
BlsI GCNGC 4 cut(s) 457, 485, 591, 1006
BmcAI AGTACT 1 cut(s) 244
Bme1390I CCNGG 2 cut(s) 51, 935
Bme18I GGWCC 3 cut(s) 212, 554, 1039
BmeRI GACNNNNNGTC 1 cut(s) 1138
BmgT120I GGNCC 3 cut(s) 212, 554, 1039
BmiI GGNNCC 2 cut(s) 555, 659
BmrFI CCNGG 2 cut(s) 51, 935
BmrI ACTGGG 1 cut(s) 163
BmsI GCATC 6 cut(s) 340, 442, 565, 763, 862, 991
BmtI GCTAGC 2 cut(s) 426, 522
BmuI ACTGGG 1 cut(s) 163
BpiI GAAGAC 1 cut(s) 487
BpmI CTGGAG 1 cut(s) 956
Bpu14I TTCGAA 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 1047
BsaJI CCNNGG 3 cut(s) 215, 933, 1035
BsaWI WCCGGW 1 cut(s) 616
Bse118I RCCGGY 1 cut(s) 321
Bse1I ACTGG 2 cut(s) 158, 590
BseBI CCWGG 2 cut(s) 51, 935
BseDI CCNNGG 3 cut(s) 215, 933, 1035
BseGI GGATG 3 cut(s) 355, 580, 646
BseNI ACTGG 2 cut(s) 158, 590
BseXI GCAGC 4 cut(s) 442, 495, 601, 991
BsgI GTGCAG 1 cut(s) 510
BshFI GGCC 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 71
BsiSI CCGG 2 cut(s) 322, 617
BslFI GGGAC 2 cut(s) 164, 567
BsmFI GGGAC 2 cut(s) 164, 567
BsnI GGCC 1 cut(s) 134
Bsp119I TTCGAA 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 71
Bsp143I GATC 2 cut(s) 283, 925
BspACI CCGC 5 cut(s) 202, 522, 542, 817, 1072
BspANI GGCC 1 cut(s) 134
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 2 cut(s) 555, 659
BspOI GCTAGC 2 cut(s) 426, 522
BspPI GGATC 1 cut(s) 291
BspT104I TTCGAA 1 cut(s) 224
BsrBI CCGCTC 1 cut(s) 817
BsrFI RCCGGY 1 cut(s) 321
BsrI ACTGG 2 cut(s) 158, 590
BssAI RCCGGY 1 cut(s) 321
BssECI CCNNGG 3 cut(s) 215, 933, 1035
BssMI GATC 2 cut(s) 283, 925
BssT1I CCWWGG 1 cut(s) 215
Bst2UI CCWGG 2 cut(s) 51, 935
Bst4CI ACNGT 3 cut(s) 528, 707, 1162
Bst6I CTCTTC 2 cut(s) 136, 1074
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 3 cut(s) 424, 428, 520
BstDEI CTNAG 1 cut(s) 1182
BstDSI CCRYGG 1 cut(s) 1035
BstF5I GGATG 3 cut(s) 355, 580, 646
BstKTI GATC 2 cut(s) 286, 928
BstMBI GATC 2 cut(s) 283, 925
BstMWI GCNNNNNNNGC 1 cut(s) 700
BstNI CCWGG 2 cut(s) 51, 935
BstNSI RCATGY 1 cut(s) 1028
BstSCI CCNGG 2 cut(s) 49, 933
BstSFI CTRYAG 2 cut(s) 558, 867
BstV1I GCAGC 4 cut(s) 442, 495, 601, 991
BstV2I GAAGAC 1 cut(s) 487
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuRI GGCC 1 cut(s) 134
BtgI CCRYGG 1 cut(s) 1035
BtsCI GGATG 3 cut(s) 355, 580, 646
BtsIMutI CAGTG 2 cut(s) 385, 629
Cac8I GCNNGC 3 cut(s) 424, 428, 520
CaiI CAGNNNCTG 2 cut(s) 974, 1004
CciI TCATGA 1 cut(s) 463
Cfr10I RCCGGY 1 cut(s) 321
Cfr13I GGNCC 3 cut(s) 212, 554, 1039
CseI GACGC 2 cut(s) 48, 996
Csp6I GTAC 1 cut(s) 243
CspCI CAANNNNNGTGG 2 cut(s) 581, 616
CviAII CATG 6 cut(s) 5, 185, 434, 464, 980, 1025
CviQI GTAC 1 cut(s) 243
DdeI CTNAG 1 cut(s) 1182
DpnI GATC 2 cut(s) 285, 927
DpnII GATC 2 cut(s) 283, 925
DriI GACNNNNNGTC 1 cut(s) 1138
Eam1104I CTCTTC 2 cut(s) 136, 1074
Eam1105I GACNNNNNGTC 1 cut(s) 1138
EarI CTCTTC 2 cut(s) 136, 1074
Ecl136II GAGCTC 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 215
Eco24I GRGCYC 1 cut(s) 71
Eco32I GATATC 1 cut(s) 190
Eco47I GGWCC 3 cut(s) 212, 554, 1039
Eco53kI GAGCTC 1 cut(s) 69
EcoICRI GAGCTC 1 cut(s) 69
EcoO109I RGGNCCY 1 cut(s) 554
EcoRII CCWGG 2 cut(s) 49, 933
EcoRV GATATC 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 215
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 215
FaeI CATG 6 cut(s) 8, 188, 437, 467, 983, 1028
FaqI GGGAC 2 cut(s) 164, 567
FatI CATG 6 cut(s) 4, 184, 433, 463, 979, 1024
FauI CCCGC 1 cut(s) 810
FbaI TGATCA 1 cut(s) 925
FblI GTMKAC 1 cut(s) 1128
Fnu4HI GCNGC 4 cut(s) 456, 484, 590, 1005
FokI GGATG 3 cut(s) 362, 587, 653
FriOI GRGCYC 1 cut(s) 71
Fsp4HI GCNGC 4 cut(s) 456, 484, 590, 1005
FspBI CTAG 6 cut(s) 267, 326, 423, 519, 858, 1176
GluI GCNGC 4 cut(s) 456, 484, 590, 1005
GsuI CTGGAG 1 cut(s) 956
HaeIII GGCC 1 cut(s) 134
HapII CCGG 2 cut(s) 322, 617
HgaI GACGC 2 cut(s) 48, 996
Hin1II CATG 6 cut(s) 8, 188, 437, 467, 983, 1028
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 5 cut(s) 158, 311, 727, 881, 896
HpaII CCGG 2 cut(s) 322, 617
HphI GGTGA 2 cut(s) 736, 1097
Hpy166II GTNNAC 5 cut(s) 38, 127, 394, 1039, 1129
Hpy188I TCNGA 5 cut(s) 66, 250, 880, 1051, 1111
Hpy188III TCNNGA 4 cut(s) 297, 464, 710, 719
Hpy8I GTNNAC 5 cut(s) 38, 127, 394, 1039, 1129
HpyAV CCTTC 4 cut(s) 226, 565, 826, 1147
HpyCH4III ACNGT 3 cut(s) 528, 707, 1162
HpyCH4V TGCA 4 cut(s) 4, 353, 491, 776
HpyF10VI GCNNNNNNNGC 1 cut(s) 700
HpyF3I CTNAG 1 cut(s) 1182
Hsp92II CATG 6 cut(s) 8, 188, 437, 467, 983, 1028
Ksp22I TGATCA 1 cut(s) 925
Kzo9I GATC 2 cut(s) 283, 925
Lsp1109I GCAGC 4 cut(s) 442, 495, 601, 991
LweI GCATC 6 cut(s) 340, 442, 565, 763, 862, 991
MaeI CTAG 6 cut(s) 267, 326, 423, 519, 858, 1176
MaeIII GTNAC 4 cut(s) 334, 662, 697, 1085
MalI GATC 2 cut(s) 285, 927
MbiI CCGCTC 1 cut(s) 817
MboI GATC 2 cut(s) 283, 925
MboII GAAGA 7 cut(s) 150, 153, 184, 238, 252, 487, 1091
MfeI CAATTG 1 cut(s) 1143
MflI RGATCY 1 cut(s) 283
MhlI GDGCHC 1 cut(s) 71
MluCI AATT 9 cut(s) 178, 300, 356, 611, 756, 803, 909, 1094, 1143
MmeI TCCRAC 2 cut(s) 273, 1056
MnlI CCTC 9 cut(s) 57, 141, 340, 376, 441, 460, 715, 1042, 1075
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 758
MseI TTAA 6 cut(s) 303, 369, 405, 513, 846, 903
MslI CAYNNNNRTG 1 cut(s) 9
MspA1I CMGCKG 1 cut(s) 486
MspI CCGG 2 cut(s) 322, 617
MspR9I CCNGG 2 cut(s) 51, 935
MunI CAATTG 1 cut(s) 1143
MvaI CCWGG 2 cut(s) 51, 935
MwoI GCNNNNNNNGC 1 cut(s) 700
NdeII GATC 2 cut(s) 283, 925
NheI GCTAGC 2 cut(s) 422, 518
NlaIII CATG 6 cut(s) 8, 188, 437, 467, 983, 1028
NlaIV GGNNCC 2 cut(s) 555, 659
NmuCI GTSAC 4 cut(s) 334, 662, 697, 1085
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 1028
NspV TTCGAA 1 cut(s) 224
PagI TCATGA 1 cut(s) 463
PciI ACATGT 1 cut(s) 1024
PdmI GAANNNNTTC 1 cut(s) 758
PfeI GAWTC 5 cut(s) 158, 311, 727, 881, 896
PkrI GCNGC 4 cut(s) 457, 485, 591, 1006
PpuMI RGGWCCY 1 cut(s) 554
PscI ACATGT 1 cut(s) 1024
PsiI TTATAA 1 cut(s) 174
Psp124BI GAGCTC 1 cut(s) 71
Psp5II RGGWCCY 1 cut(s) 554
Psp6I CCWGG 2 cut(s) 49, 933
PspGI CCWGG 2 cut(s) 49, 933
PspN4I GGNNCC 2 cut(s) 555, 659
PspPI GGNCC 3 cut(s) 212, 554, 1039
PspPPI RGGWCCY 1 cut(s) 554
PsrI GAACNNNNNNTAC 2 cut(s) 627, 659
PstNI CAGNNNCTG 2 cut(s) 974, 1004
PsuI RGATCY 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 486
RsaI GTAC 1 cut(s) 244
RsaNI GTAC 1 cut(s) 243
RseI CAYNNNNRTG 1 cut(s) 9
SacI GAGCTC 1 cut(s) 71
SaqAI TTAA 6 cut(s) 303, 369, 405, 513, 846, 903
SatI GCNGC 4 cut(s) 456, 484, 590, 1005
Sau3AI GATC 2 cut(s) 283, 925
Sau96I GGNCC 3 cut(s) 212, 554, 1039
ScaI AGTACT 1 cut(s) 244
ScrFI CCNGG 2 cut(s) 51, 935
SduI GDGCHC 1 cut(s) 71
SfaNI GCATC 6 cut(s) 340, 442, 565, 763, 862, 991
SfcI CTRYAG 2 cut(s) 558, 867
SfuI TTCGAA 1 cut(s) 224
SinI GGWCC 3 cut(s) 212, 554, 1039
SmiMI CAYNNNNRTG 1 cut(s) 9
SmlI CTYRAG 1 cut(s) 1062
SmoI CTYRAG 1 cut(s) 1062
SpeI ACTAGT 1 cut(s) 1175
Sse9I AATT 9 cut(s) 178, 300, 356, 611, 756, 803, 909, 1094, 1143
SsiI CCGC 5 cut(s) 202, 522, 542, 817, 1072
SspMI CTAG 6 cut(s) 267, 326, 423, 519, 858, 1176
SstI GAGCTC 1 cut(s) 71
StyD4I CCNGG 2 cut(s) 49, 933
StyI CCWWGG 1 cut(s) 215
TaaI ACNGT 3 cut(s) 528, 707, 1162
TaqI TCGA 2 cut(s) 224, 709
TasI AATT 9 cut(s) 178, 300, 356, 611, 756, 803, 909, 1094, 1143
TatI WGTACW 1 cut(s) 242
TfiI GAWTC 5 cut(s) 158, 311, 727, 881, 896
Tru1I TTAA 6 cut(s) 303, 369, 405, 513, 846, 903
Tru9I TTAA 6 cut(s) 303, 369, 405, 513, 846, 903
TscAI CASTG 2 cut(s) 385, 629
TseFI GTSAC 4 cut(s) 334, 662, 697, 1085
TseI GCWGC 4 cut(s) 455, 483, 589, 1004
Tsp45I GTSAC 4 cut(s) 334, 662, 697, 1085
TspDTI ATGAA 6 cut(s) 78, 84, 245, 252, 303, 921
TspGWI ACGGA 3 cut(s) 225, 650, 1024
TspRI CASTG 2 cut(s) 385, 629
VpaK11BI GGWCC 3 cut(s) 212, 554, 1039
XapI RAATTY 4 cut(s) 356, 756, 803, 909
XceI RCATGY 1 cut(s) 1028
XmiI GTMKAC 1 cut(s) 1128
XmnI GAANNNNTTC 1 cut(s) 758
XspI CTAG 6 cut(s) 267, 326, 423, 519, 858, 1176
ZrmI AGTACT 1 cut(s) 244
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.