Rh4DG001300

YT521-B-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005296
Physical Location & Seq
Forward (+)
283984 .. 290082
6099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG001300.1

Sequence Viewer

Length: 1695 bp
ATGTCTTATAGTGGTTATGGAAGTAGCACTGGTACTTGGGATGGATATCCTCAGTATATCAATACTGATGGAATGCATGTTGTATCACCGGTAATGTACAGTGATAATCCATCTCTTCTGTATCACTCTGGTTATGGTTTCAATCCCGAAATTGCATACGGACCATATTCACCGGTTGCTACACCTTTGGCTTCTTATTTGGTAGATGGCCAGCTGTATTCTCCACAACAGGTCCCCTTCTCTCCATCTTATTACCCTCAACCATCTCCTCCAAGTCTGTCTCATATTACTTCAGCTATTCCAGTGACACCAACTGAGCGGATGACCTCAGAAAGTAGTAGCACTGACAACATGGCCTTTGGGCCAGGATCAGGTTACTTTGTAAATTTTAGATCATTTAGTGGAGGAGATCTATCTGGAAATCTTGGTTCGAGCCCTTTAGCATCTCCAGGGATTTACCCTCAGCCAATGGGCATACTTGGATCTTATGAGCACAGTGTTGGGCAGATTTCTCATCCACAGAGATCAATGCATGGATTTGGACTGGTATCTAGTTCCTTTACAGGGCGCTACCCACATAATACTTCTCATCAGAGCTCCAACTTTGGTGGTCCTTCTTACTCGTTTGGTAATGACCGGAGCCGACTGACTGTTGACAAGGGTAGAAGAAGAGATAGGGAGTGGGATTCAATTTCCGTTTTCAGTAATTCACATGATATCTCCAATGACCGTAATCGAGGACCAAGGGCTTCAAAGCTGAAGGACAAGAACTTGTCTGACCAGGGCTCTTCATCTAGTGCTAGGAAAATGGATCTATCTGCTTCTGGAATTAACCTCGACTCAATTAACCGGAAGGATTTTGTCACAGATTATGAGGAGGCAAAGTTCTTTATCATTAAGTCTTTCAGTGAGGATAATGTTCATAAAAGTATTAAATACAGTGTTTGGGCCAGCACTCCCCATGGAAACAAGAAGCTTGATGCTGCTTATCATGAGGCGAAGAGGATAAAAAGCAGCTGTCCAGTCTTTCTCTTATTTTCGGTTAATGCCAGTGGACAGTTTTGCGGAGTAGCCGAAATGATTGGACCAGTAGACTTTGGAAAAGATGCTGATTACTGGCAGCAAGACAGGTGGAATGGCCAATTTAAAGTTCAGTGGCACATTATTAAGGATGTTCCTAACATTCGCTTCCGTCACATTCTACTTGAAAATAATGATAATAAGCCTGTCACTCACAGCCGAGATTGTCAGGAGGTGAATCTGAAACAAGGAATTGAGCTGTTGAAAATTTTCAATGATTATGATGCACGGACATCTATAGTTGATGATTTTGAATTTTATGATGACCGCGAAAGGTCCTTGAAGGAAAGGAAAGCCAGACAACAAGCTTGTGCAACTACAGATGCTTCTGAGTCAGTTGTTCTTGGTACTGTTAAGGAAGCTTCTAATATATCCGATCAAGCCTTGGAGATGAAAGGAAGTAGTAGCAAAGAATTTGCAGGAACTGAACATGACAGTAGCTCCAAAACAGATTCAGTTGTATATGAACATGATGCTGTACAGCAAATATCTGATAGTTTATCTCAAGTATTGCAGTTGGAAGAGAGTGACAAAGAGGTAGTACCACCATCTGAAAGAGGCGGCACACATGACAGCGAGTCCGTTGACAAGGAGGTAACAGTTGTATCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YTH

Protein Analysis

564

Amino Acids

62.39

Weight (kDa)

5.58

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YTH PF04146 294 - 432 3.7e-50 YT521-B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 319
AccI GTMKAC 1 cut(s) 1092
AccII CGCG 1 cut(s) 1350
AciI CCGC 4 cut(s) 319, 1065, 1348, 1641
AclWI GGATC 3 cut(s) 376, 490, 819
AcoI YGGCCR 2 cut(s) 208, 1138
AcsI RAATTY 4 cut(s) 385, 1287, 1334, 1493
AcuI CTGAAG 2 cut(s) 276, 779
AfaI GTAC 5 cut(s) 34, 98, 1429, 1560, 1623
AfiI CCNNNNNNNGG 2 cut(s) 371, 564
AgeI ACCGGT 2 cut(s) 88, 172
AgsI TTSAA 8 cut(s) 142, 690, 753, 1208, 1285, 1294, 1334, 1363
AhdI GACNNNNNGTC 1 cut(s) 1657
AjnI CCWGG 3 cut(s) 364, 448, 780
AjuI GAANNNNNNNTTGG 2 cut(s) 1134, 1166
AloI GAACNNNNNNTCC 2 cut(s) 869, 901
Alw21I GWGCWC 2 cut(s) 495, 599
Alw26I GTCTC 1 cut(s) 285
AlwI GGATC 3 cut(s) 376, 490, 819
AlwNI CAGNNNCTG 1 cut(s) 1505
AoxI GGCC 5 cut(s) 208, 354, 362, 948, 1138
ApeKI GCWGC 3 cut(s) 983, 1014, 1120
ApoI RAATTY 4 cut(s) 385, 1287, 1334, 1493
AsiGI ACCGGT 2 cut(s) 88, 172
Asp700I GAANNNNTTC 1 cut(s) 1289
AspLEI GCGC 1 cut(s) 570
AspS9I GGNCC 8 cut(s) 161, 232, 362, 611, 740, 948, 1085, 1356
AsuHPI GGTGA 3 cut(s) 78, 162, 1267
AvaII GGWCC 6 cut(s) 161, 232, 611, 740, 1085, 1356
BaeI ACNNNNGTAYC 1 cut(s) 1668
BalI TGGCCA 2 cut(s) 210, 1140
BanII GRGCYC 3 cut(s) 437, 599, 788
Bbv12I GWGCWC 2 cut(s) 495, 599
BbvCI CCTCAGC 1 cut(s) 462
BbvI GCAGC 3 cut(s) 970, 1026, 1132
BccI CCATC 7 cut(s) 35, 62, 118, 200, 253, 271, 1636
BciT130I CCWGG 3 cut(s) 366, 450, 782
BcoDI GTCTC 1 cut(s) 285
BfaI CTAG 3 cut(s) 552, 795, 801
BfmI CTRYAG 2 cut(s) 1317, 1398
BfoI RGCGCY 1 cut(s) 571
BglII AGATCT 1 cut(s) 409
BisI GCNGC 4 cut(s) 984, 1015, 1121, 1642
BlsI GCNGC 4 cut(s) 985, 1016, 1122, 1643
Bme1390I CCNGG 3 cut(s) 366, 450, 782
Bme18I GGWCC 6 cut(s) 161, 232, 611, 740, 1085, 1356
BmeRI GACNNNNNGTC 1 cut(s) 1657
BmgT120I GGNCC 8 cut(s) 161, 232, 362, 611, 740, 948, 1085, 1356
BmiI GGNNCC 2 cut(s) 234, 641
BmrFI CCNGG 3 cut(s) 366, 450, 782
BmsI GCATC 6 cut(s) 452, 970, 1096, 1294, 1393, 1543
BpmI CTGGAG 1 cut(s) 432
Bpu10I CCTNAGC 1 cut(s) 462
BpuEI CTTGAG 1 cut(s) 1569
BsaBI GATNNNNATC 1 cut(s) 45
BsaJI CCNNGG 5 cut(s) 449, 743, 781, 961, 1464
BsaWI WCCGGW 4 cut(s) 88, 172, 636, 849
BsaXI ACNNNNNCTCC 4 cut(s) 869, 899, 1505, 1535
Bsc4I CCNNNNNNNGG 2 cut(s) 371, 564
Bse118I RCCGGY 2 cut(s) 88, 172
Bse1I ACTGG 7 cut(s) 34, 302, 549, 1022, 1050, 1088, 1121
Bse8I GATNNNNATC 1 cut(s) 45
BseBI CCWGG 3 cut(s) 366, 450, 782
BseDI CCNNGG 5 cut(s) 449, 743, 781, 961, 1464
BseGI GGATG 4 cut(s) 46, 327, 514, 1177
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 2 cut(s) 371, 564
BseMII CTCAG 5 cut(s) 65, 306, 342, 476, 1401
BseNI ACTGG 7 cut(s) 34, 302, 549, 1022, 1050, 1088, 1121
BseRI GAGGAG 3 cut(s) 258, 420, 890
BseXI GCAGC 3 cut(s) 970, 1026, 1132
Bsh1236I CGCG 1 cut(s) 1350
BshFI GGCC 5 cut(s) 210, 356, 364, 950, 1140
BshTI ACCGGT 2 cut(s) 88, 172
BsiHKAI GWGCWC 2 cut(s) 495, 599
BsiSI CCGG 4 cut(s) 89, 173, 637, 850
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 2 cut(s) 371, 564
BsmAI GTCTC 1 cut(s) 285
BsmFI GGGAC 1 cut(s) 218
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 5 cut(s) 210, 356, 364, 950, 1140
Bsp1286I GDGCHC 4 cut(s) 437, 495, 599, 788
Bsp1407I TGTACA 2 cut(s) 96, 1558
Bsp143I GATC 7 cut(s) 368, 392, 409, 482, 524, 811, 1456
Bsp19I CCATGG 1 cut(s) 961
BspACI CCGC 4 cut(s) 319, 1065, 1348, 1641
BspANI GGCC 5 cut(s) 210, 356, 364, 950, 1140
BspCNI CTCAG 5 cut(s) 64, 307, 341, 475, 1402
BspFNI CGCG 1 cut(s) 1350
BspHI TCATGA 1 cut(s) 991
BspLI GGNNCC 2 cut(s) 234, 641
BspPI GGATC 3 cut(s) 376, 490, 819
BspQI GCTCTTC 1 cut(s) 793
BsrBI CCGCTC 1 cut(s) 319
BsrFI RCCGGY 2 cut(s) 88, 172
BsrGI TGTACA 2 cut(s) 96, 1558
BsrI ACTGG 7 cut(s) 34, 302, 549, 1022, 1050, 1088, 1121
BssAI RCCGGY 2 cut(s) 88, 172
BssECI CCNNGG 5 cut(s) 449, 743, 781, 961, 1464
BssMI GATC 7 cut(s) 368, 392, 409, 482, 524, 811, 1456
BssT1I CCWWGG 3 cut(s) 743, 961, 1464
Bst2UI CCWGG 3 cut(s) 366, 450, 782
Bst4CI ACNGT 9 cut(s) 101, 497, 652, 731, 941, 1059, 1432, 1517, 1681
Bst6I CTCTTC 5 cut(s) 120, 664, 793, 995, 1596
BstAUI TGTACA 2 cut(s) 96, 1558
BstC8I GCNNGC 2 cut(s) 212, 952
BstDEI CTNAG 5 cut(s) 51, 315, 328, 462, 1410
BstDSI CCRYGG 1 cut(s) 961
BstF5I GGATG 4 cut(s) 46, 327, 514, 1177
BstFNI CGCG 1 cut(s) 1350
BstH2I RGCGCY 1 cut(s) 571
BstHHI GCGC 1 cut(s) 570
BstKTI GATC 7 cut(s) 371, 395, 412, 485, 527, 814, 1459
BstMAI GTCTC 1 cut(s) 285
BstMBI GATC 7 cut(s) 368, 392, 409, 482, 524, 811, 1456
BstNI CCWGG 3 cut(s) 366, 450, 782
BstNSI RCATGY 1 cut(s) 80
BstSCI CCNGG 3 cut(s) 364, 448, 780
BstSFI CTRYAG 2 cut(s) 1317, 1398
BstUI CGCG 1 cut(s) 1350
BstV1I GCAGC 3 cut(s) 970, 1026, 1132
BstX2I RGATCY 3 cut(s) 409, 482, 811
BstYI RGATCY 3 cut(s) 409, 482, 811
BsuRI GGCC 5 cut(s) 210, 356, 364, 950, 1140
BtgI CCRYGG 1 cut(s) 961
BtsCI GGATG 4 cut(s) 46, 327, 514, 1177
BtsIMutI CAGTG 9 cut(s) 27, 106, 309, 342, 502, 913, 946, 1057, 1160
Cac8I GCNNGC 2 cut(s) 212, 952
CaiI CAGNNNCTG 1 cut(s) 1505
CciI TCATGA 1 cut(s) 991
CfoI GCGC 1 cut(s) 570
Cfr10I RCCGGY 2 cut(s) 88, 172
Cfr13I GGNCC 8 cut(s) 161, 232, 362, 611, 740, 948, 1085, 1356
Csp6I GTAC 5 cut(s) 33, 97, 1428, 1559, 1622
CspAI ACCGGT 2 cut(s) 88, 172
CspCI CAANNNNNGTGG 4 cut(s) 589, 624, 1112, 1147
CviAII CATG 9 cut(s) 77, 352, 533, 713, 962, 992, 1511, 1550, 1649
CviQI GTAC 5 cut(s) 33, 97, 1428, 1559, 1622
DdeI CTNAG 5 cut(s) 51, 315, 328, 462, 1410
DpnI GATC 7 cut(s) 370, 394, 411, 484, 526, 813, 1458
DpnII GATC 7 cut(s) 368, 392, 409, 482, 524, 811, 1456
DraI TTTAAA 1 cut(s) 1147
DriI GACNNNNNGTC 1 cut(s) 1657
EaeI YGGCCR 2 cut(s) 208, 1138
Eam1104I CTCTTC 5 cut(s) 120, 664, 793, 995, 1596
Eam1105I GACNNNNNGTC 1 cut(s) 1657
EarI CTCTTC 5 cut(s) 120, 664, 793, 995, 1596
Ecl136II GAGCTC 1 cut(s) 597
Eco130I CCWWGG 3 cut(s) 743, 961, 1464
Eco24I GRGCYC 3 cut(s) 437, 599, 788
Eco32I GATATC 2 cut(s) 47, 718
Eco47I GGWCC 6 cut(s) 161, 232, 611, 740, 1085, 1356
Eco53kI GAGCTC 1 cut(s) 597
Eco57I CTGAAG 2 cut(s) 276, 779
EcoICRI GAGCTC 1 cut(s) 597
EcoO109I RGGNCCY 2 cut(s) 232, 1356
EcoRII CCWGG 3 cut(s) 364, 448, 780
EcoRV GATATC 2 cut(s) 47, 718
EcoT14I CCWWGG 3 cut(s) 743, 961, 1464
EcoT22I ATGCAT 2 cut(s) 78, 534
EcoT38I GRGCYC 3 cut(s) 437, 599, 788
ErhI CCWWGG 3 cut(s) 743, 961, 1464
FaeI CATG 9 cut(s) 80, 355, 536, 716, 965, 995, 1514, 1553, 1652
FaqI GGGAC 1 cut(s) 218
FatI CATG 9 cut(s) 76, 351, 532, 712, 961, 991, 1510, 1549, 1648
FblI GTMKAC 1 cut(s) 1092
Fnu4HI GCNGC 4 cut(s) 984, 1015, 1121, 1642
FokI GGATG 4 cut(s) 53, 334, 501, 1184
FriOI GRGCYC 3 cut(s) 437, 599, 788
Fsp4HI GCNGC 4 cut(s) 984, 1015, 1121, 1642
FspBI CTAG 3 cut(s) 552, 795, 801
GlaI GCGC 1 cut(s) 569
GluI GCNGC 4 cut(s) 984, 1015, 1121, 1642
GsuI CTGGAG 1 cut(s) 432
HaeII RGCGCY 1 cut(s) 571
HaeIII GGCC 5 cut(s) 210, 356, 364, 950, 1140
HapII CCGG 4 cut(s) 89, 173, 637, 850
HhaI GCGC 1 cut(s) 570
Hin1II CATG 9 cut(s) 80, 355, 536, 716, 965, 995, 1514, 1553, 1652
Hin6I GCGC 1 cut(s) 568
HinP1I GCGC 1 cut(s) 568
HincII GTYRAC 2 cut(s) 655, 1666
HindII GTYRAC 2 cut(s) 655, 1666
HindIII AAGCTT 3 cut(s) 974, 1386, 1440
HinfI GANTC 6 cut(s) 686, 839, 1258, 1412, 1532, 1658
HpaII CCGG 4 cut(s) 89, 173, 637, 850
HphI GGTGA 3 cut(s) 78, 162, 1267
Hpy166II GTNNAC 4 cut(s) 655, 1055, 1093, 1666
Hpy188I TCNGA 8 cut(s) 331, 594, 778, 1263, 1411, 1456, 1573, 1633
Hpy188III TCNNGA 5 cut(s) 146, 417, 825, 992, 1250
Hpy8I GTNNAC 4 cut(s) 655, 1055, 1093, 1666
HpyAV CCTTC 5 cut(s) 247, 624, 754, 847, 1357
HpyCH4III ACNGT 9 cut(s) 101, 497, 652, 731, 941, 1059, 1432, 1517, 1681
HpyCH4V TGCA 7 cut(s) 76, 155, 532, 1307, 1394, 1499, 1594
HpyF3I CTNAG 5 cut(s) 51, 315, 328, 462, 1410
Hsp92II CATG 9 cut(s) 80, 355, 536, 716, 965, 995, 1514, 1553, 1652
HspAI GCGC 1 cut(s) 568
Kzo9I GATC 7 cut(s) 368, 392, 409, 482, 524, 811, 1456
LguI GCTCTTC 1 cut(s) 793
LmnI GCTCC 3 cut(s) 602, 639, 1526
Lsp1109I GCAGC 3 cut(s) 970, 1026, 1132
LweI GCATC 6 cut(s) 452, 970, 1096, 1294, 1393, 1543
MaeI CTAG 3 cut(s) 552, 795, 801
MaeIII GTNAC 7 cut(s) 304, 374, 862, 1193, 1228, 1607, 1675
MalI GATC 7 cut(s) 370, 394, 411, 484, 526, 813, 1458
MbiI CCGCTC 1 cut(s) 319
MboI GATC 7 cut(s) 368, 392, 409, 482, 524, 811, 1456
MboII GAAGA 6 cut(s) 107, 678, 681, 780, 1012, 1613
MflI RGATCY 3 cut(s) 409, 482, 811
MhlI GDGCHC 4 cut(s) 437, 495, 599, 788
MlsI TGGCCA 2 cut(s) 210, 1140
MluNI TGGCCA 2 cut(s) 210, 1140
MlyI GAGTC 3 cut(s) 833, 1421, 1667
MmeI TCCRAC 2 cut(s) 624, 1578
Mox20I TGGCCA 2 cut(s) 210, 1140
Mph1103I ATGCAT 2 cut(s) 78, 534
MroXI GAANNNNTTC 1 cut(s) 1289
MscI TGGCCA 2 cut(s) 210, 1140
MseI TTAA 9 cut(s) 831, 846, 897, 933, 1044, 1146, 1167, 1434, 1693
Msp20I TGGCCA 2 cut(s) 210, 1140
MspA1I CMGCKG 2 cut(s) 214, 1017
MspI CCGG 4 cut(s) 89, 173, 637, 850
MspR9I CCNGG 3 cut(s) 366, 450, 782
Mva1269I GAATGC 1 cut(s) 78
MvaI CCWGG 3 cut(s) 366, 450, 782
MvnI CGCG 1 cut(s) 1350
NcoI CCATGG 1 cut(s) 961
NdeII GATC 7 cut(s) 368, 392, 409, 482, 524, 811, 1456
NlaIII CATG 9 cut(s) 80, 355, 536, 716, 965, 995, 1514, 1553, 1652
NlaIV GGNNCC 2 cut(s) 234, 641
NmeAIII GCCGAG 1 cut(s) 1265
NmuCI GTSAC 5 cut(s) 304, 862, 1193, 1228, 1607
NsiI ATGCAT 2 cut(s) 78, 534
NspI RCATGY 1 cut(s) 80
PagI TCATGA 1 cut(s) 991
PciSI GCTCTTC 1 cut(s) 793
PctI GAATGC 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 1289
PfeI GAWTC 3 cut(s) 686, 1258, 1532
PinAI ACCGGT 2 cut(s) 88, 172
PkrI GCNGC 4 cut(s) 985, 1016, 1122, 1643
PleI GAGTC 3 cut(s) 833, 1420, 1666
PpsI GAGTC 3 cut(s) 833, 1420, 1666
PpuMI RGGWCCY 2 cut(s) 232, 1356
Psp124BI GAGCTC 1 cut(s) 599
Psp5II RGGWCCY 2 cut(s) 232, 1356
Psp6I CCWGG 3 cut(s) 364, 448, 780
PspGI CCWGG 3 cut(s) 364, 448, 780
PspN4I GGNNCC 2 cut(s) 234, 641
PspPI GGNCC 8 cut(s) 161, 232, 362, 611, 740, 948, 1085, 1356
PspPPI RGGWCCY 2 cut(s) 232, 1356
PstNI CAGNNNCTG 1 cut(s) 1505
PsuI RGATCY 3 cut(s) 409, 482, 811
PvuII CAGCTG 2 cut(s) 214, 1017
RsaI GTAC 5 cut(s) 34, 98, 1429, 1560, 1623
RsaNI GTAC 5 cut(s) 33, 97, 1428, 1559, 1622
SacI GAGCTC 1 cut(s) 599
SapI GCTCTTC 1 cut(s) 793
SaqAI TTAA 9 cut(s) 831, 846, 897, 933, 1044, 1146, 1167, 1434, 1693
SatI GCNGC 4 cut(s) 984, 1015, 1121, 1642
Sau3AI GATC 7 cut(s) 368, 392, 409, 482, 524, 811, 1456
Sau96I GGNCC 8 cut(s) 161, 232, 362, 611, 740, 948, 1085, 1356
SchI GAGTC 3 cut(s) 833, 1421, 1667
ScrFI CCNGG 3 cut(s) 366, 450, 782
SduI GDGCHC 4 cut(s) 437, 495, 599, 788
SfaNI GCATC 6 cut(s) 452, 970, 1096, 1294, 1393, 1543
SfcI CTRYAG 2 cut(s) 1317, 1398
SinI GGWCC 6 cut(s) 161, 232, 611, 740, 1085, 1356
SmlI CTYRAG 1 cut(s) 1584
SmoI CTYRAG 1 cut(s) 1584
SsiI CCGC 4 cut(s) 319, 1065, 1348, 1641
SspMI CTAG 3 cut(s) 552, 795, 801
SstI GAGCTC 1 cut(s) 599
StyD4I CCNGG 3 cut(s) 364, 448, 780
StyI CCWWGG 3 cut(s) 743, 961, 1464
TaaI ACNGT 9 cut(s) 101, 497, 652, 731, 941, 1059, 1432, 1517, 1681
TaqI TCGA 3 cut(s) 431, 736, 837
TatI WGTACW 2 cut(s) 96, 1558
TauI GCSGC 1 cut(s) 1644
TfiI GAWTC 3 cut(s) 686, 1258, 1532
Tru1I TTAA 9 cut(s) 831, 846, 897, 933, 1044, 1146, 1167, 1434, 1693
Tru9I TTAA 9 cut(s) 831, 846, 897, 933, 1044, 1146, 1167, 1434, 1693
TscAI CASTG 9 cut(s) 34, 106, 309, 349, 502, 913, 946, 1057, 1160
TseFI GTSAC 5 cut(s) 304, 862, 1193, 1228, 1607
TseI GCWGC 3 cut(s) 983, 1014, 1120
Tsp45I GTSAC 5 cut(s) 304, 862, 1193, 1228, 1607
TspDTI ATGAA 4 cut(s) 780, 911, 1487, 1560
TspGWI ACGGA 5 cut(s) 174, 685, 1181, 1324, 1651
TspRI CASTG 9 cut(s) 34, 106, 309, 349, 502, 913, 946, 1057, 1160
VpaK11BI GGWCC 6 cut(s) 161, 232, 611, 740, 1085, 1356
XapI RAATTY 4 cut(s) 385, 1287, 1334, 1493
XceI RCATGY 1 cut(s) 80
XmiI GTMKAC 1 cut(s) 1092
XmnI GAANNNNTTC 1 cut(s) 1289
XspI CTAG 3 cut(s) 552, 795, 801
Zsp2I ATGCAT 2 cut(s) 78, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.