pycom04g00640

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005611
Physical Location & Seq
Forward (+)
615381 .. 617326
1946 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1143 bp
ATGGCATGCCAAATGATACATTCGTGGAAATTTAACGAGCTAGTCGGTGCATTTCTTGATCTTGCAATAGCCTACCTTCTACTATGTGCCGCGGCAGTTGCCTACTTTACCTCGAAATTTGTTGGTCTGTTTGGGTTTTGTCTGCCGTGCCCTTGTGATGGGTTTTTTGGGACCCCAAGGAAAAGTAACTGCTTTCAGAGGCAATTTGCGGATGCCCCGTGTGAGAAAATCTCTTCAGTTCTATGGTCCGTTAGGAGTAAGTTCCCTTTCGATGTTATATGGGATGAAGACCCAAATTTCCATTCGAAGTTGAAGTCCCTAAATGAGGCAAATGGGAGTTTTGCGTTTGAAGGTGAAGCGTCATGTAGCTCATTTTCGGTGGCTGAGAATGAACAGAGTGTTGAGTATGGTGTAGGCAACTTGGCAGCTGGGAAGGAACGGAGTTTTGATCTTAAGGCAAAGAAGGTTGCAGGTCGAAGGCCGAGACTTCGGTGCCGCCGCAGAGGGGGCTCTGTTGATTATGGAAATTCATCTTCAGTTTCATCATATAATATGTTCCAGTCCGCTGCAGAGGACAATCCCACATCTCCTTCCAGCATTAGTAAAATGGGAAATGAGGTTACTGAAGTTTTTGTAAATTCAGATGGCATTGAAGCTCGTACAGATATGAGTTCGCCTGAAAGCGTTTCACGTGTTGGGTTTAATGAACATGCTGATGAAACTAAATCAGTGGAGAAGGATGGATCACTTGTTGAAGATTCTGGATCCAATGCAGGAGAGAAACTAGGTTTTGACAGTAATGAGACAACTACATTTAGAGTGCTGGAACAGGCACTCGAAGAAGAGCATGCTGCTCGTGCTGCTCTGTATCTTGAACTGGAGAAGGAGAGAAGTGCTGCTGCCACCGCTGCAGATGAGGCCATGGCCATGATAATGCGCCTGCAAGAGGAGAAGGCATCAATAGAAATGGAGGCAATGCAGTACCAAAGGATGATAGAGGAAAAATTTGCTTACGATGCTGAGGAAATGAACATTCTTACAGAGATCTTAATTAGGAGGGAGAGGGAAAAGCATTTCTTGGAGAACGAAGTTGAGCAATACAGGCAAATGTTTTTTGGAGATCGATCAAGTGGATTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

381

Amino Acids

42.47

Weight (kDa)

4.91

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 275 - 364 1.2e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 461
AccB1I GGYRCC 1 cut(s) 492
AccII CGCG 1 cut(s) 92
AciI CCGC 7 cut(s) 90, 92, 209, 496, 499, 564, 906
AclWI GGATC 3 cut(s) 751, 759, 772
AcoI YGGCCR 1 cut(s) 924
AcsI RAATTY 6 cut(s) 29, 116, 295, 526, 637, 1004
AcuI CTGAAG 3 cut(s) 219, 519, 645
AcvI CACGTG 1 cut(s) 692
AfaI GTAC 2 cut(s) 661, 983
AfiI CCNNNNNNNGG 4 cut(s) 158, 325, 505, 946
AflII CTTAAG 1 cut(s) 452
AflIII ACRYGT 1 cut(s) 691
AgsI TTSAA 6 cut(s) 313, 350, 653, 755, 875, 1138
AjuI GAANNNNNNNTTGG 2 cut(s) 286, 318
AluBI AGCT 4 cut(s) 40, 369, 428, 656
AluI AGCT 4 cut(s) 40, 369, 428, 656
Alw26I GTCTC 2 cut(s) 478, 797
AlwI GGATC 3 cut(s) 751, 759, 772
AoxI GGCC 3 cut(s) 479, 918, 924
ApeKI GCWGC 7 cut(s) 425, 566, 851, 860, 896, 899, 908
ApoI RAATTY 6 cut(s) 29, 116, 295, 526, 637, 1004
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
AspLEI GCGC 1 cut(s) 939
AspS9I GGNCC 2 cut(s) 171, 246
AsuHPI GGTGA 1 cut(s) 365
AsuII TTCGAA 1 cut(s) 305
AvaII GGWCC 2 cut(s) 171, 246
BaeGI GKGCMC 1 cut(s) 152
BaeI ACNNNNGTAYC 2 cut(s) 8, 41
BalI TGGCCA 1 cut(s) 926
BamHI GGATCC 1 cut(s) 764
BanI GGYRCC 1 cut(s) 492
BanII GRGCYC 1 cut(s) 512
BauI CACGAG 1 cut(s) 855
BbrPI CACGTG 1 cut(s) 692
BbsI GAAGAC 1 cut(s) 294
BbvCI CCTCAGC 1 cut(s) 1020
BbvI GCAGC 7 cut(s) 437, 553, 838, 847, 883, 886, 895
BccI CCATC 3 cut(s) 152, 638, 734
BceAI ACGGC 1 cut(s) 130
BcgI CGANNNNNNTGC 2 cut(s) 836, 870
BcoDI GTCTC 2 cut(s) 478, 797
BfaI CTAG 2 cut(s) 41, 785
BfmI CTRYAG 2 cut(s) 567, 909
BfrI CTTAAG 1 cut(s) 452
BfuAI ACCTGC 1 cut(s) 461
BglII AGATCT 1 cut(s) 1044
Bme18I GGWCC 2 cut(s) 171, 246
BmgT120I GGNCC 2 cut(s) 171, 246
BmiI GGNNCC 4 cut(s) 172, 173, 494, 766
BmsI GCATC 3 cut(s) 202, 965, 1006
BpiI GAAGAC 1 cut(s) 294
BplI GAGNNNNNCTC 2 cut(s) 215, 247
BpmI CTGGAG 1 cut(s) 899
Bpu10I CCTNAGC 1 cut(s) 1020
Bpu14I TTCGAA 1 cut(s) 305
Bsa29I ATCGAT 1 cut(s) 1123
BsaAI YACGTR 1 cut(s) 692
BsaJI CCNNGG 3 cut(s) 90, 176, 921
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 325, 505, 946
Bse1I ACTGG 2 cut(s) 559, 882
Bse3DI GCAATG 1 cut(s) 981
BseCI ATCGAT 1 cut(s) 1123
BseDI CCNNGG 3 cut(s) 90, 176, 921
BseGI GGATG 4 cut(s) 217, 289, 745, 996
BseLI CCNNNNNNNGG 4 cut(s) 158, 325, 505, 946
BseMI GCAATG 1 cut(s) 981
BseMII CTCAG 2 cut(s) 375, 1011
BseNI ACTGG 2 cut(s) 559, 882
BseRI GAGGAG 1 cut(s) 962
BseSI GKGCMC 1 cut(s) 152
BseXI GCAGC 7 cut(s) 437, 553, 838, 847, 883, 886, 895
BseYI CCCAGC 1 cut(s) 428
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 3 cut(s) 481, 920, 926
BshNI GGYRCC 1 cut(s) 492
BshVI ATCGAT 1 cut(s) 1123
BslFI GGGAC 2 cut(s) 184, 301
BslI CCNNNNNNNGG 4 cut(s) 158, 325, 505, 946
BsmAI GTCTC 2 cut(s) 478, 797
BsmFI GGGAC 2 cut(s) 184, 301
BsnI GGCC 3 cut(s) 481, 920, 926
Bsp119I TTCGAA 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 152, 512
Bsp143I GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
Bsp19I CCATGG 1 cut(s) 921
BspACI CCGC 7 cut(s) 90, 92, 209, 496, 499, 564, 906
BspANI GGCC 3 cut(s) 481, 920, 926
BspCNI CTCAG 2 cut(s) 376, 1012
BspDI ATCGAT 1 cut(s) 1123
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 4 cut(s) 172, 173, 494, 766
BspMAI CTGCAG 2 cut(s) 571, 913
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 3 cut(s) 751, 759, 772
BspQI GCTCTTC 1 cut(s) 837
BspT104I TTCGAA 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 492
BspTI CTTAAG 1 cut(s) 452
BsrDI GCAATG 1 cut(s) 981
BsrI ACTGG 2 cut(s) 559, 882
BssECI CCNNGG 3 cut(s) 90, 176, 921
BssMI GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
BssSI CACGAG 1 cut(s) 855
BssT1I CCWWGG 2 cut(s) 176, 921
Bst2BI CACGAG 1 cut(s) 855
Bst4CI ACNGT 1 cut(s) 797
Bst6I CTCTTC 2 cut(s) 238, 837
BstAFI CTTAAG 1 cut(s) 452
BstBAI YACGTR 1 cut(s) 692
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 3 cut(s) 7, 849, 941
BstDEI CTNAG 2 cut(s) 384, 1020
BstDSI CCRYGG 2 cut(s) 90, 921
BstENI CCTNNNNNAGG 2 cut(s) 323, 944
BstF5I GGATG 4 cut(s) 217, 289, 745, 996
BstFNI CGCG 1 cut(s) 92
BstHHI GCGC 1 cut(s) 939
BstKTI GATC 7 cut(s) 61, 451, 746, 767, 1047, 1123, 1127
BstMAI GTCTC 2 cut(s) 478, 797
BstMBI GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
BstMWI GCNNNNNNNGC 9 cut(s) 98, 507, 857, 860, 905, 908, 917, 1016, 1102
BstNSI RCATGY 3 cut(s) 9, 713, 851
BstSFI CTRYAG 2 cut(s) 567, 909
BstSLI GKGCMC 1 cut(s) 152
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 7 cut(s) 437, 553, 838, 847, 883, 886, 895
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 2 cut(s) 764, 1044
BstYI RGATCY 2 cut(s) 764, 1044
Bsu15I ATCGAT 1 cut(s) 1123
BsuRI GGCC 3 cut(s) 481, 920, 926
BsuTUI ATCGAT 1 cut(s) 1123
BtgI CCRYGG 2 cut(s) 90, 921
BtsCI GGATG 4 cut(s) 217, 289, 745, 996
BtsIMutI CAGTG 1 cut(s) 735
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 3 cut(s) 7, 849, 941
CfoI GCGC 1 cut(s) 939
Cfr13I GGNCC 2 cut(s) 171, 246
Cfr42I CCGCGG 1 cut(s) 93
ClaI ATCGAT 1 cut(s) 1123
CseI GACGC 1 cut(s) 348
Csp6I GTAC 2 cut(s) 660, 982
CviAII CATG 6 cut(s) 6, 363, 710, 848, 922, 928
CviQI GTAC 2 cut(s) 660, 982
DdeI CTNAG 2 cut(s) 384, 1020
DpnI GATC 7 cut(s) 60, 450, 745, 766, 1046, 1122, 1126
DpnII GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
EaeI YGGCCR 1 cut(s) 924
Eam1104I CTCTTC 2 cut(s) 238, 837
EarI CTCTTC 2 cut(s) 238, 837
Eco130I CCWWGG 2 cut(s) 176, 921
Eco24I GRGCYC 1 cut(s) 512
Eco47I GGWCC 2 cut(s) 171, 246
Eco57I CTGAAG 3 cut(s) 219, 519, 645
Eco72I CACGTG 1 cut(s) 692
EcoNI CCTNNNNNAGG 2 cut(s) 323, 944
EcoO109I RGGNCCY 1 cut(s) 171
EcoT14I CCWWGG 2 cut(s) 176, 921
EcoT38I GRGCYC 1 cut(s) 512
ErhI CCWWGG 2 cut(s) 176, 921
FaeI CATG 6 cut(s) 9, 366, 713, 851, 925, 931
FalI AAGNNNNNCTT 2 cut(s) 1061, 1093
FaqI GGGAC 2 cut(s) 184, 301
FatI CATG 6 cut(s) 5, 362, 709, 847, 921, 927
FokI GGATG 4 cut(s) 224, 296, 752, 1003
FriOI GRGCYC 1 cut(s) 512
FspBI CTAG 2 cut(s) 41, 785
GlaI GCGC 1 cut(s) 938
GsaI CCCAGC 1 cut(s) 432
GsuI CTGGAG 1 cut(s) 899
HaeIII GGCC 3 cut(s) 481, 920, 926
HgaI GACGC 1 cut(s) 348
HhaI GCGC 1 cut(s) 939
Hin1II CATG 6 cut(s) 9, 366, 713, 851, 925, 931
Hin6I GCGC 1 cut(s) 937
HinP1I GCGC 1 cut(s) 937
HinfI GANTC 2 cut(s) 758, 1134
HphI GGTGA 1 cut(s) 365
Hpy188I TCNGA 2 cut(s) 198, 643
Hpy188III TCNNGA 3 cut(s) 56, 762, 872
HpyAV CCTTC 9 cut(s) 86, 344, 427, 457, 471, 600, 730, 877, 946
HpyCH4III ACNGT 1 cut(s) 797
HpyCH4IV ACGT 1 cut(s) 691
HpyCH4V TGCA 8 cut(s) 50, 65, 470, 569, 773, 911, 943, 979
HpyF10VI GCNNNNNNNGC 9 cut(s) 98, 507, 857, 860, 905, 908, 917, 1016, 1102
HpyF3I CTNAG 2 cut(s) 384, 1020
HpySE526I ACGT 1 cut(s) 691
Hsp92II CATG 6 cut(s) 9, 366, 713, 851, 925, 931
HspAI GCGC 1 cut(s) 937
KflI GGGWCCC 1 cut(s) 171
KspI CCGCGG 1 cut(s) 93
Kzo9I GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
LguI GCTCTTC 1 cut(s) 837
Lsp1109I GCAGC 7 cut(s) 437, 553, 838, 847, 883, 886, 895
LweI GCATC 3 cut(s) 202, 965, 1006
MaeI CTAG 2 cut(s) 41, 785
MaeII ACGT 1 cut(s) 691
MaeIII GTNAC 2 cut(s) 185, 619
MalI GATC 7 cut(s) 60, 450, 745, 766, 1046, 1122, 1126
MboI GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
MboII GAAGA 6 cut(s) 225, 299, 525, 767, 851, 854
MflI RGATCY 2 cut(s) 764, 1044
MhlI GDGCHC 2 cut(s) 152, 512
MlsI TGGCCA 1 cut(s) 926
MluCI AATT 8 cut(s) 29, 116, 203, 295, 526, 637, 1004, 1050
MluNI TGGCCA 1 cut(s) 926
Mox20I TGGCCA 1 cut(s) 926
MscI TGGCCA 1 cut(s) 926
MseI TTAA 4 cut(s) 33, 453, 702, 1049
MslI CAYNNNNRTG 3 cut(s) 714, 926, 932
Msp20I TGGCCA 1 cut(s) 926
MspA1I CMGCKG 4 cut(s) 92, 428, 566, 908
MspCI CTTAAG 1 cut(s) 452
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 9 cut(s) 98, 507, 857, 860, 905, 908, 917, 1016, 1102
NcoI CCATGG 1 cut(s) 921
NdeII GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
NlaIII CATG 6 cut(s) 9, 366, 713, 851, 925, 931
NlaIV GGNNCC 4 cut(s) 172, 173, 494, 766
NmeAIII GCCGAG 1 cut(s) 507
NspI RCATGY 3 cut(s) 9, 713, 851
NspV TTCGAA 1 cut(s) 305
PaeI GCATGC 2 cut(s) 9, 851
PciSI GCTCTTC 1 cut(s) 837
PfeI GAWTC 2 cut(s) 758, 1134
PmaCI CACGTG 1 cut(s) 692
PmlI CACGTG 1 cut(s) 692
Ppu21I YACGTR 1 cut(s) 692
PpuMI RGGWCCY 1 cut(s) 171
Psp5II RGGWCCY 1 cut(s) 171
PspCI CACGTG 1 cut(s) 692
PspFI CCCAGC 1 cut(s) 428
PspN4I GGNNCC 4 cut(s) 172, 173, 494, 766
PspPI GGNCC 2 cut(s) 171, 246
PspPPI RGGWCCY 1 cut(s) 171
PstI CTGCAG 2 cut(s) 571, 913
PsuI RGATCY 2 cut(s) 764, 1044
PvuII CAGCTG 1 cut(s) 428
RsaI GTAC 2 cut(s) 661, 983
RsaNI GTAC 2 cut(s) 660, 982
RseI CAYNNNNRTG 3 cut(s) 714, 926, 932
SacII CCGCGG 1 cut(s) 93
SapI GCTCTTC 1 cut(s) 837
SaqAI TTAA 4 cut(s) 33, 453, 702, 1049
Sau3AI GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
Sau96I GGNCC 2 cut(s) 171, 246
SduI GDGCHC 2 cut(s) 152, 512
SfaNI GCATC 3 cut(s) 202, 965, 1006
SfcI CTRYAG 2 cut(s) 567, 909
Sfr303I CCGCGG 1 cut(s) 93
SfuI TTCGAA 1 cut(s) 305
SgrBI CCGCGG 1 cut(s) 93
SinI GGWCC 2 cut(s) 171, 246
SmiMI CAYNNNNRTG 3 cut(s) 714, 926, 932
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
SphI GCATGC 2 cut(s) 9, 851
Sse9I AATT 8 cut(s) 29, 116, 203, 295, 526, 637, 1004, 1050
SsiI CCGC 7 cut(s) 90, 92, 209, 496, 499, 564, 906
SspMI CTAG 2 cut(s) 41, 785
StyI CCWWGG 2 cut(s) 176, 921
TaaI ACNGT 1 cut(s) 797
TaiI ACGT 1 cut(s) 694
TaqI TCGA 6 cut(s) 113, 270, 305, 475, 837, 1123
TasI AATT 8 cut(s) 29, 116, 203, 295, 526, 637, 1004, 1050
TauI GCSGC 4 cut(s) 92, 95, 498, 501
TfiI GAWTC 2 cut(s) 758, 1134
Tru1I TTAA 4 cut(s) 33, 453, 702, 1049
Tru9I TTAA 4 cut(s) 33, 453, 702, 1049
TscAI CASTG 1 cut(s) 735
TseI GCWGC 7 cut(s) 425, 566, 851, 860, 896, 899, 908
TspDTI ATGAA 7 cut(s) 300, 405, 519, 531, 720, 732, 1043
TspGWI ACGGA 2 cut(s) 238, 454
TspRI CASTG 1 cut(s) 735
Vha464I CTTAAG 1 cut(s) 452
VpaK11BI GGWCC 2 cut(s) 171, 246
XagI CCTNNNNNAGG 2 cut(s) 323, 944
XapI RAATTY 6 cut(s) 29, 116, 295, 526, 637, 1004
XceI RCATGY 3 cut(s) 9, 713, 851
XspI CTAG 2 cut(s) 41, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.