pycom04g01230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005914
Physical Location & Seq
Reverse (-)
1106873 .. 1108255
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g01230.1

Sequence Viewer

Length: 1383 bp
ATGATGCCCTCGAGAAGAAAGAAGTGGACAGAGTCCGAGGAGAGAACTCCGATCGACAGATATGGAGAGATGGTCTCTGATGGGACCCTTGCTAAGATGAAAACCAGAGAGAAAAAGTTTAAACCCATTGCCTGCTATGTGAATTCTGTGCACCATGTTCGAGACCCGATTGCGTATCGTTGGCAGTGGTCTTGGAAGGATGTTTCCACCAAGGTCCAGAACATGAGGCACCAGTATTTGCTTGTCAAGCAGAAGATTAAGAAGCAGCAACCCGAGTGTAATTCGGGGGGCGGTGGGGGTGGTGGTGGACCTGGTGGTGGTGGTAGTAGTGGTGGTGGGAGTGGGGAGTGTAGTGAGGAGGAGTTTGATTGGATGGAGGGCCTCGCGCATTGGTCGAATTTTTTGAGGTATAAGGAGGTTTTTGGGGATGTGGAGATTGTGAGTAATAGTGGTAATGATTTGATGGTGTCTGTGAATGGGGATGGTGAGAATGGTGGGAGGTTCGTGGGGAGTGGGAGGGGAATGGGGCTTGTGGACTTTGGGCAATTGGGGCATTCAGATGGGGATTTTGGAGCGGGGATTGATGGGGTTGAGAATGCAGTGATGGGTTTGGGATTTGAGTTTGACCGGGAGGAGGGAGAGGTGAATTTTAATCGTAATGATCGGTTGAGGCAGGATGGAGAGGACAAGTTTGTGTACGAAGAAGTTGATCAAAATGGTCATAATTTAAAGAAGAGGAAGAAGAAGAGGGACGTGTCGACGGGGTTGGGAAAGAAGAAGGCATGGGCCTTTCTAGGTAACCAACTGGGCAAGTTGAAGAAAATGGAGGCACGGTTTGAGCATCGCGAGGCAGAGAGAGAACGGGACAGACGGAGGAGTGAGAATTTTAGAATGGAGTGTGAGCAAGAACGAGAGCGGAAATGGGAGGAATGGGCAAATAAAAAGGAGACGCAAGAGAAGACAAGAGAGAAGTTAAAGAAGCAGAGGATTCTAGAATGGGAAGCATTGGAGAAGGAGAGTGAAGAGAGAGAAAGAAGAAGAAAAGAGGACGAGTTGATTCATGAGAGGGAATGGGAGGAGAGGATGAATAGGAGGAGAACAGAATGGAAGACGAGGATTGACGAAATGTTGGCTCACCACCGGGCAGAGATGGGCCAGATGCAGACTCGTATTCTTCACGAGCAACAAAACCTTACAGGCCAGTTACTTGGGATTGTCTCTCAGTGGCCTGCTCAGCCAGCTGGACAGTCTGATCATACAAGTGCTAGTAACCATTATTTATCACAAATGATGCAGAATTTGCACCATGTGAACATTTTGGTTCATGATGATGCTAGGGTTGACGGAGAAAATCAAGATGATCAATACATTCTCGATGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

461

Amino Acids

53.39

Weight (kDa)

6.39

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 228
AccBSI CCGCTC 2 cut(s) 575, 916
AccI GTMKAC 1 cut(s) 758
AccII CGCG 2 cut(s) 386, 846
AciI CCGC 3 cut(s) 291, 575, 916
AcsI RAATTY 5 cut(s) 142, 397, 646, 883, 1297
AdeI CACNNNGTG 1 cut(s) 1309
AfaI GTAC 1 cut(s) 698
AfiI CCNNNNNNNGG 2 cut(s) 317, 634
AflIII ACRYGT 1 cut(s) 753
AgsI TTSAA 1 cut(s) 817
AjiI CACGTC 1 cut(s) 754
AjnI CCWGG 1 cut(s) 310
AluBI AGCT 1 cut(s) 1241
AluI AGCT 1 cut(s) 1241
Alw21I GWGCWC 1 cut(s) 153
Alw26I GTCTC 4 cut(s) 79, 156, 941, 1222
Alw44I GTGCAC 1 cut(s) 149
Ama87I CYCGRG 2 cut(s) 10, 272
AoxI GGCC 5 cut(s) 379, 786, 1153, 1198, 1226
ApaLI GTGCAC 1 cut(s) 149
ApeKI GCWGC 1 cut(s) 265
ApoI RAATTY 5 cut(s) 142, 397, 646, 883, 1297
AspLEI GCGC 1 cut(s) 388
AspS9I GGNCC 6 cut(s) 84, 214, 308, 379, 786, 1153
AsuC2I CCSGG 2 cut(s) 629, 1142
AsuHPI GGTGA 3 cut(s) 497, 655, 1127
AvaI CYCGRG 2 cut(s) 10, 272
AvaII GGWCC 3 cut(s) 84, 214, 308
BaeGI GKGCMC 1 cut(s) 153
BanI GGYRCC 1 cut(s) 228
BauI CACGAG 1 cut(s) 1178
BbsI GAAGAC 2 cut(s) 965, 1115
Bbv12I GWGCWC 1 cut(s) 153
BbvI GCAGC 1 cut(s) 277
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciT130I CCWGG 1 cut(s) 312
BclI TGATCA 3 cut(s) 709, 1252, 1360
BcnI CCSGG 2 cut(s) 629, 1142
BcoDI GTCTC 4 cut(s) 79, 156, 941, 1222
BfaI CTAG 4 cut(s) 794, 992, 1266, 1335
BisI GCNGC 1 cut(s) 266
BlpI GCTNAGC 1 cut(s) 1233
BlsI GCNGC 1 cut(s) 267
Bme1390I CCNGG 3 cut(s) 312, 629, 1142
Bme18I GGWCC 3 cut(s) 84, 214, 308
BmeT110I CYCGRG 2 cut(s) 10, 272
BmgBI CACGTC 1 cut(s) 754
BmgT120I GGNCC 6 cut(s) 84, 214, 308, 379, 786, 1153
BmiI GGNNCC 3 cut(s) 85, 86, 230
BmrFI CCNGG 3 cut(s) 312, 629, 1142
BmrI ACTGGG 1 cut(s) 815
BmsI GCATC 4 cut(s) 850, 1149, 1281, 1321
BmuI ACTGGG 1 cut(s) 815
BpiI GAAGAC 2 cut(s) 965, 1115
BplI GAGNNNNNCTC 2 cut(s) 59, 91
Bpu1102I GCTNAGC 1 cut(s) 1233
BpuMI CCSGG 2 cut(s) 629, 1142
BsaI GGTCTC 2 cut(s) 79, 156
BsaJI CCNNGG 2 cut(s) 36, 210
BsaXI ACNNNNNCTCC 4 cut(s) 57, 87, 818, 848
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 634
Bse1I ACTGG 3 cut(s) 232, 810, 1201
Bse3DI GCAATG 1 cut(s) 126
BseBI CCWGG 1 cut(s) 312
BseDI CCNNGG 2 cut(s) 36, 210
BseGI GGATG 6 cut(s) 205, 378, 433, 487, 682, 1089
BseLI CCNNNNNNNGG 2 cut(s) 317, 634
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 2 cut(s) 1235, 1247
BseNI ACTGG 3 cut(s) 232, 810, 1201
BseRI GAGGAG 7 cut(s) 53, 371, 374, 647, 889, 1091, 1108
BseSI GKGCMC 1 cut(s) 153
BseXI GCAGC 1 cut(s) 277
Bsh1236I CGCG 2 cut(s) 386, 846
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 5 cut(s) 381, 788, 1155, 1200, 1228
BshNI GGYRCC 1 cut(s) 228
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 153
BsiHKCI CYCGRG 2 cut(s) 10, 272
BsiSI CCGG 2 cut(s) 628, 1141
BslFI GGGAC 3 cut(s) 97, 764, 878
BslI CCNNNNNNNGG 2 cut(s) 317, 634
BsmAI GTCTC 4 cut(s) 79, 156, 941, 1222
BsmBI CGTCTC 1 cut(s) 941
BsmFI GGGAC 3 cut(s) 97, 764, 878
BsmI GAATGC 2 cut(s) 553, 601
BsnI GGCC 5 cut(s) 381, 788, 1155, 1200, 1228
Bso31I GGTCTC 2 cut(s) 79, 156
BsoBI CYCGRG 2 cut(s) 10, 272
Bsp1286I GDGCHC 1 cut(s) 153
Bsp143I GATC 5 cut(s) 51, 661, 709, 1252, 1360
Bsp1720I GCTNAGC 1 cut(s) 1233
Bsp68I TCGCGA 1 cut(s) 846
BspACI CCGC 3 cut(s) 291, 575, 916
BspANI GGCC 5 cut(s) 381, 788, 1155, 1200, 1228
BspCNI CTCAG 2 cut(s) 1234, 1246
BspFNI CGCG 2 cut(s) 386, 846
BspHI TCATGA 2 cut(s) 1060, 1324
BspLI GGNNCC 3 cut(s) 85, 86, 230
BspT107I GGYRCC 1 cut(s) 228
BspTNI GGTCTC 2 cut(s) 79, 156
BsrBI CCGCTC 2 cut(s) 575, 916
BsrDI GCAATG 1 cut(s) 126
BsrI ACTGG 3 cut(s) 232, 810, 1201
BssECI CCNNGG 2 cut(s) 36, 210
BssMI GATC 5 cut(s) 51, 661, 709, 1252, 1360
BssSI CACGAG 1 cut(s) 1178
BssT1I CCWWGG 1 cut(s) 210
Bst2BI CACGAG 1 cut(s) 1178
Bst2UI CCWGG 1 cut(s) 312
Bst4CI ACNGT 2 cut(s) 834, 1248
Bst6I CTCTTC 3 cut(s) 728, 740, 1017
BstAPI GCANNNNNTGC 1 cut(s) 1300
BstC8I GCNNGC 3 cut(s) 133, 1230, 1239
BstDEI CTNAG 3 cut(s) 93, 1221, 1233
BstEII GGTNACC 1 cut(s) 797
BstF5I GGATG 6 cut(s) 205, 378, 433, 487, 682, 1089
BstFNI CGCG 2 cut(s) 386, 846
BstHHI GCGC 1 cut(s) 388
BstKTI GATC 5 cut(s) 54, 664, 712, 1255, 1363
BstMAI GTCTC 4 cut(s) 79, 156, 941, 1222
BstMBI GATC 5 cut(s) 51, 661, 709, 1252, 1360
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 5 cut(s) 247, 550, 1234, 1238, 1300
BstNI CCWGG 1 cut(s) 312
BstPI GGTNACC 1 cut(s) 797
BstSCI CCNGG 3 cut(s) 310, 627, 1140
BstSLI GKGCMC 1 cut(s) 153
BstUI CGCG 2 cut(s) 386, 846
BstV1I GCAGC 1 cut(s) 277
BstV2I GAAGAC 2 cut(s) 965, 1115
BstXI CCANNNNNNTGG 1 cut(s) 1208
BsuRI GGCC 5 cut(s) 381, 788, 1155, 1200, 1228
BtgZI GCGATG 1 cut(s) 827
BtrI CACGTC 1 cut(s) 754
BtsCI GGATG 6 cut(s) 205, 378, 433, 487, 682, 1089
BtsI GCAGTG 2 cut(s) 191, 606
BtsIMutI CAGTG 3 cut(s) 191, 606, 1229
BtuMI TCGCGA 1 cut(s) 846
Cac8I GCNNGC 3 cut(s) 133, 1230, 1239
CciI TCATGA 2 cut(s) 1060, 1324
CfoI GCGC 1 cut(s) 388
Cfr13I GGNCC 6 cut(s) 84, 214, 308, 379, 786, 1153
CseI GACGC 1 cut(s) 958
CsiI ACCWGGT 1 cut(s) 310
Csp6I GTAC 1 cut(s) 697
CviAII CATG 6 cut(s) 155, 223, 783, 1061, 1307, 1325
CviJI RGCY 9 cut(s) 381, 529, 788, 1133, 1155, 1200, 1228, 1237, 1241
CviKI_1 RGCY 9 cut(s) 381, 529, 788, 1133, 1155, 1200, 1228, 1237, 1241
CviQI GTAC 1 cut(s) 697
DdeI CTNAG 3 cut(s) 93, 1221, 1233
DpnI GATC 5 cut(s) 53, 663, 711, 1254, 1362
DpnII GATC 5 cut(s) 51, 661, 709, 1252, 1360
DraI TTTAAA 2 cut(s) 121, 729
DraIII CACNNNGTG 1 cut(s) 1309
Eam1104I CTCTTC 3 cut(s) 728, 740, 1017
EarI CTCTTC 3 cut(s) 728, 740, 1017
Eco130I CCWWGG 1 cut(s) 210
Eco31I GGTCTC 2 cut(s) 79, 156
Eco47I GGWCC 3 cut(s) 84, 214, 308
Eco88I CYCGRG 2 cut(s) 10, 272
Eco91I GGTNACC 1 cut(s) 797
EcoO109I RGGNCCY 2 cut(s) 84, 379
EcoO65I GGTNACC 1 cut(s) 797
EcoRI GAATTC 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 310
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
Esp3I CGTCTC 1 cut(s) 941
FaeI CATG 6 cut(s) 158, 226, 786, 1064, 1310, 1328
FaqI GGGAC 3 cut(s) 97, 764, 878
FatI CATG 6 cut(s) 154, 222, 782, 1060, 1306, 1324
FauI CCCGC 1 cut(s) 568
FbaI TGATCA 3 cut(s) 709, 1252, 1360
FblI GTMKAC 1 cut(s) 758
Fnu4HI GCNGC 1 cut(s) 266
FokI GGATG 6 cut(s) 212, 385, 440, 494, 689, 1096
Fsp4HI GCNGC 1 cut(s) 266
FspBI CTAG 4 cut(s) 794, 992, 1266, 1335
GlaI GCGC 1 cut(s) 387
GluI GCNGC 1 cut(s) 266
HaeIII GGCC 5 cut(s) 381, 788, 1155, 1200, 1228
HapII CCGG 2 cut(s) 628, 1141
HgaI GACGC 1 cut(s) 958
HhaI GCGC 1 cut(s) 388
Hin1II CATG 6 cut(s) 158, 226, 786, 1064, 1310, 1328
Hin6I GCGC 1 cut(s) 386
HinP1I GCGC 1 cut(s) 386
HincII GTYRAC 2 cut(s) 759, 1342
HindII GTYRAC 2 cut(s) 759, 1342
HinfI GANTC 4 cut(s) 32, 988, 1057, 1165
HpaII CCGG 2 cut(s) 628, 1141
HphI GGTGA 3 cut(s) 497, 655, 1127
Hpy166II GTNNAC 8 cut(s) 27, 151, 308, 535, 697, 759, 1312, 1342
Hpy188I TCNGA 5 cut(s) 37, 51, 79, 559, 1252
Hpy8I GTNNAC 8 cut(s) 27, 151, 308, 535, 697, 759, 1312, 1342
Hpy99I CGWCG 1 cut(s) 763
HpyAV CCTTC 3 cut(s) 190, 772, 1006
HpyCH4III ACNGT 2 cut(s) 834, 1248
HpyCH4IV ACGT 1 cut(s) 753
HpyCH4V TGCA 5 cut(s) 151, 599, 1162, 1294, 1303
HpyF10VI GCNNNNNNNGC 5 cut(s) 247, 550, 1234, 1238, 1300
HpyF3I CTNAG 3 cut(s) 93, 1221, 1233
HpySE526I ACGT 1 cut(s) 753
Hsp92II CATG 6 cut(s) 158, 226, 786, 1064, 1310, 1328
HspAI GCGC 1 cut(s) 386
KflI GGGWCCC 1 cut(s) 84
Ksp22I TGATCA 3 cut(s) 709, 1252, 1360
Kzo9I GATC 5 cut(s) 51, 661, 709, 1252, 1360
LmnI GCTCC 1 cut(s) 572
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 4 cut(s) 850, 1149, 1281, 1321
MabI ACCWGGT 1 cut(s) 310
MaeI CTAG 4 cut(s) 794, 992, 1266, 1335
MaeII ACGT 1 cut(s) 753
MaeIII GTNAC 3 cut(s) 797, 1203, 1268
MalI GATC 5 cut(s) 53, 663, 711, 1254, 1362
MbiI CCGCTC 2 cut(s) 575, 916
MboI GATC 5 cut(s) 51, 661, 709, 1252, 1360
MfeI CAATTG 1 cut(s) 545
MhlI GDGCHC 1 cut(s) 153
MluCI AATT 8 cut(s) 142, 280, 397, 545, 646, 724, 883, 1297
MlyI GAGTC 2 cut(s) 41, 1159
MseI TTAA 5 cut(s) 120, 258, 651, 728, 974
MslI CAYNNNNRTG 3 cut(s) 558, 1260, 1329
MspA1I CMGCKG 1 cut(s) 1241
MspI CCGG 2 cut(s) 628, 1141
MspR9I CCNGG 3 cut(s) 312, 629, 1142
MssI GTTTAAAC 1 cut(s) 121
MunI CAATTG 1 cut(s) 545
Mva1269I GAATGC 2 cut(s) 553, 601
MvaI CCWGG 1 cut(s) 312
MvnI CGCG 2 cut(s) 386, 846
MwoI GCNNNNNNNGC 5 cut(s) 247, 550, 1234, 1238, 1300
NciI CCSGG 2 cut(s) 629, 1142
NdeII GATC 5 cut(s) 51, 661, 709, 1252, 1360
NlaIII CATG 6 cut(s) 158, 226, 786, 1064, 1310, 1328
NlaIV GGNNCC 3 cut(s) 85, 86, 230
NruI TCGCGA 1 cut(s) 846
PaeR7I CTCGAG 1 cut(s) 10
PagI TCATGA 2 cut(s) 1060, 1324
PctI GAATGC 2 cut(s) 553, 601
PfeI GAWTC 2 cut(s) 988, 1057
PflFI GACNNNGTC 1 cut(s) 31
PkrI GCNGC 1 cut(s) 267
Ple19I CGATCG 1 cut(s) 54
PleI GAGTC 2 cut(s) 40, 1159
PmeI GTTTAAAC 1 cut(s) 121
PpsI GAGTC 2 cut(s) 40, 1159
PpuMI RGGWCCY 1 cut(s) 84
Psp5II RGGWCCY 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 310
PspEI GGTNACC 1 cut(s) 797
PspGI CCWGG 1 cut(s) 310
PspN4I GGNNCC 3 cut(s) 85, 86, 230
PspPI GGNCC 6 cut(s) 84, 214, 308, 379, 786, 1153
PspPPI RGGWCCY 1 cut(s) 84
PsyI GACNNNGTC 1 cut(s) 31
PvuI CGATCG 1 cut(s) 54
PvuII CAGCTG 1 cut(s) 1241
RruI TCGCGA 1 cut(s) 846
RsaI GTAC 1 cut(s) 698
RsaNI GTAC 1 cut(s) 697
RseI CAYNNNNRTG 3 cut(s) 558, 1260, 1329
SalI GTCGAC 1 cut(s) 757
SaqAI TTAA 5 cut(s) 120, 258, 651, 728, 974
SatI GCNGC 1 cut(s) 266
Sau3AI GATC 5 cut(s) 51, 661, 709, 1252, 1360
Sau96I GGNCC 6 cut(s) 84, 214, 308, 379, 786, 1153
SchI GAGTC 2 cut(s) 41, 1159
ScrFI CCNGG 3 cut(s) 312, 629, 1142
SduI GDGCHC 1 cut(s) 153
SexAI ACCWGGT 1 cut(s) 310
SfaNI GCATC 4 cut(s) 850, 1149, 1281, 1321
Sfr274I CTCGAG 1 cut(s) 10
SinI GGWCC 3 cut(s) 84, 214, 308
SlaI CTCGAG 1 cut(s) 10
SmiMI CAYNNNNRTG 3 cut(s) 558, 1260, 1329
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 8 cut(s) 142, 280, 397, 545, 646, 724, 883, 1297
SsiI CCGC 3 cut(s) 291, 575, 916
SspMI CTAG 4 cut(s) 794, 992, 1266, 1335
StyD4I CCNGG 3 cut(s) 310, 627, 1140
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 2 cut(s) 834, 1248
TaiI ACGT 1 cut(s) 756
TaqI TCGA 6 cut(s) 11, 54, 160, 395, 758, 1374
TasI AATT 8 cut(s) 142, 280, 397, 545, 646, 724, 883, 1297
TfiI GAWTC 2 cut(s) 988, 1057
Tru1I TTAA 5 cut(s) 120, 258, 651, 728, 974
Tru9I TTAA 5 cut(s) 120, 258, 651, 728, 974
TscAI CASTG 3 cut(s) 191, 606, 1229
TseI GCWGC 1 cut(s) 265
TspDTI ATGAA 4 cut(s) 113, 1049, 1100, 1313
TspGWI ACGGA 2 cut(s) 886, 1359
TspRI CASTG 3 cut(s) 191, 606, 1229
Tth111I GACNNNGTC 1 cut(s) 31
VneI GTGCAC 1 cut(s) 149
VpaK11BI GGWCC 3 cut(s) 84, 214, 308
XapI RAATTY 5 cut(s) 142, 397, 646, 883, 1297
XbaI TCTAGA 1 cut(s) 991
XhoI CTCGAG 1 cut(s) 10
XmiI GTMKAC 1 cut(s) 758
XspI CTAG 4 cut(s) 794, 992, 1266, 1335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.