pycom04g03180

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001712
Physical Location & Seq
Reverse (-)
2925200 .. 2926744
1545 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 849 bp
ATGCTTCTCTATCAATCACATGCAAACAAATCTCCACCCAACAACATGATGGACCCATACACCCCACAGTTCCACTTTGGGCCCCACCCTCTCTCCTATTGTCCTATCCTACATTTCGTGTCCAATCGTCCACCACGTGTCCACTTCTCATTTTTGTCATCATCAACCCCTTTCAACCTCTCGCTACAGCTGTCCCGTCCAGCTTTAAAGGCACGTCTCTCTGCGCCTCTCTCAAACACTCACGAGAAAAGAAAAGTCCTAGAGAGAGAAAGCTCGAGAAAGTTTGAGAGAGAGAGAGAGAGAGAAATGGAGGCCTTCGTTTGCGAAACAGAGTCATGGGTTTCTTCAATCAATGAAATCCCAGATGAGCAAACCAATCAATTGCTTCGAACATCGTCATCTTCTTCCGGATTCACCCAAGAAACCGACGAGGAAGAATGGCCGAAGCCTAACAGGTTGAAGAGCAAGAAGAAGAAGAAGAAGCAGGTTTTGCTTGAAGGGTATGTGGAGGCGGCGGACGACGATGATCTGTCGAGGACCAAGAGCTTGACGGACGAGGATCTCGACGAGCTTAAAGGGTGTTTGGATCTGGGATTCGGGTTCAGCTACGAAGAGATTCCGGAGCTCTGTAACACCTTGCCGGCTCTCGAGCTTTGTTACTCTATGAGCCAGAAGTTCATGGACGAGCACCAGAAGTCGCCGGAGAACTCTCCGGCCGCGGTCGATTCGGTTTCGAGTCAAATTGCCAGTTGGAAGATCTCTAGTCCTGGTGACCATCCCGAGGATGTGAAAGAAAGGCTCAAATACTGGGCACAGGCCGTGGCGTGCACTGTCAAATTATGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

283

Amino Acids

32.21

Weight (kDa)

5.59

Isoelectric Point (pI)

47.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 183 - 234 4.5e-25 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 475
AccII CGCG 1 cut(s) 719
AccIII TCCGGA 2 cut(s) 407, 619
AciI CCGC 4 cut(s) 512, 515, 717, 719
AclWI GGATC 2 cut(s) 567, 594
AcoI YGGCCR 2 cut(s) 440, 714
AcvI CACGTG 1 cut(s) 137
AdeI CACNNNGTG 1 cut(s) 137
AfiI CCNNNNNNNGG 1 cut(s) 781
AflIII ACRYGT 1 cut(s) 136
AgsI TTSAA 4 cut(s) 175, 348, 460, 497
AjiI CACGTC 1 cut(s) 215
AjnI CCWGG 1 cut(s) 766
AluBI AGCT 8 cut(s) 190, 203, 273, 546, 571, 606, 625, 652
AluI AGCT 8 cut(s) 190, 203, 273, 546, 571, 606, 625, 652
Alw21I GWGCWC 3 cut(s) 627, 690, 830
Alw26I GTCTC 1 cut(s) 221
Alw44I GTGCAC 1 cut(s) 826
AlwI GGATC 2 cut(s) 567, 594
Ama87I CYCGRG 3 cut(s) 274, 647, 779
Aor13HI TCCGGA 2 cut(s) 407, 619
AoxI GGCC 5 cut(s) 80, 312, 440, 714, 816
ApaI GGGCCC 1 cut(s) 84
ApaLI GTGCAC 1 cut(s) 826
Asp700I GAANNNNTTC 1 cut(s) 615
AspLEI GCGC 1 cut(s) 226
AspS9I GGNCC 4 cut(s) 52, 80, 81, 537
AsuHPI GGTGA 2 cut(s) 406, 782
AsuII TTCGAA 1 cut(s) 388
AvaI CYCGRG 3 cut(s) 274, 647, 779
AvaII GGWCC 2 cut(s) 52, 537
BaeGI GKGCMC 3 cut(s) 84, 814, 830
BanII GRGCYC 2 cut(s) 84, 627
BauI CACGAG 1 cut(s) 242
BbrPI CACGTG 1 cut(s) 137
Bbv12I GWGCWC 3 cut(s) 627, 690, 830
BccI CCATC 2 cut(s) 43, 783
BceAI ACGGC 1 cut(s) 803
BciT130I CCWGG 1 cut(s) 768
BcoDI GTCTC 1 cut(s) 221
BfaI CTAG 2 cut(s) 260, 762
BfmI CTRYAG 1 cut(s) 185
BfuAI ACCTGC 1 cut(s) 475
BglII AGATCT 1 cut(s) 756
BisI GCNGC 2 cut(s) 513, 717
BlsI GCNGC 2 cut(s) 514, 718
Bme1390I CCNGG 1 cut(s) 768
Bme18I GGWCC 2 cut(s) 52, 537
BmeT110I CYCGRG 3 cut(s) 274, 647, 779
BmgBI CACGTC 1 cut(s) 215
BmgT120I GGNCC 4 cut(s) 52, 80, 81, 537
BmiI GGNNCC 3 cut(s) 54, 82, 83
BmrFI CCNGG 1 cut(s) 768
BmrI ACTGGG 1 cut(s) 817
BmuI ACTGGG 1 cut(s) 817
Bpu14I TTCGAA 1 cut(s) 388
BsaAI YACGTR 1 cut(s) 137
BsaJI CCNNGG 3 cut(s) 717, 780, 819
BsaWI WCCGGW 2 cut(s) 407, 619
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 1 cut(s) 781
Bse118I RCCGGY 1 cut(s) 640
Bse1I ACTGG 2 cut(s) 747, 812
BseAI TCCGGA 2 cut(s) 407, 619
BseBI CCWGG 1 cut(s) 768
BseDI CCNNGG 3 cut(s) 717, 780, 819
BseGI GGATG 2 cut(s) 775, 790
BseLI CCNNNNNNNGG 1 cut(s) 781
BseNI ACTGG 2 cut(s) 747, 812
BseSI GKGCMC 3 cut(s) 84, 814, 830
BseX3I CGGCCG 1 cut(s) 714
Bsh1236I CGCG 1 cut(s) 719
Bsh1285I CGRYCG 2 cut(s) 717, 723
BshFI GGCC 5 cut(s) 82, 314, 442, 716, 818
BsiEI CGRYCG 2 cut(s) 717, 723
BsiHKAI GWGCWC 3 cut(s) 627, 690, 830
BsiHKCI CYCGRG 3 cut(s) 274, 647, 779
BsiSI CCGG 5 cut(s) 408, 620, 641, 701, 713
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 1 cut(s) 781
BsmAI GTCTC 1 cut(s) 221
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 178
BsnI GGCC 5 cut(s) 82, 314, 442, 716, 818
BsoBI CYCGRG 3 cut(s) 274, 647, 779
Bsp119I TTCGAA 1 cut(s) 388
Bsp120I GGGCCC 1 cut(s) 80
Bsp1286I GDGCHC 5 cut(s) 84, 627, 690, 814, 830
Bsp13I TCCGGA 2 cut(s) 407, 619
Bsp143I GATC 4 cut(s) 526, 559, 586, 756
BspACI CCGC 4 cut(s) 512, 515, 717, 719
BspANI GGCC 5 cut(s) 82, 314, 442, 716, 818
BspEI TCCGGA 2 cut(s) 407, 619
BspFNI CGCG 1 cut(s) 719
BspLI GGNNCC 3 cut(s) 54, 82, 83
BspMI ACCTGC 1 cut(s) 475
BspPI GGATC 2 cut(s) 567, 594
BspQI GCTCTTC 1 cut(s) 455
BspT104I TTCGAA 1 cut(s) 388
BsrFI RCCGGY 1 cut(s) 640
BsrI ACTGG 2 cut(s) 747, 812
BssAI RCCGGY 1 cut(s) 640
BssECI CCNNGG 3 cut(s) 717, 780, 819
BssMI GATC 4 cut(s) 526, 559, 586, 756
BssSI CACGAG 1 cut(s) 242
Bst2BI CACGAG 1 cut(s) 242
Bst2UI CCWGG 1 cut(s) 768
Bst4CI ACNGT 2 cut(s) 69, 832
Bst6I CTCTTC 2 cut(s) 455, 606
BstAPI GCANNNNNTGC 1 cut(s) 490
BstBAI YACGTR 1 cut(s) 137
BstBI TTCGAA 1 cut(s) 388
BstC8I GCNNGC 2 cut(s) 642, 826
BstDSI CCRYGG 2 cut(s) 717, 819
BstEII GGTNACC 1 cut(s) 770
BstF5I GGATG 2 cut(s) 775, 790
BstFNI CGCG 1 cut(s) 719
BstHHI GCGC 1 cut(s) 226
BstKTI GATC 4 cut(s) 529, 562, 589, 759
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 4 cut(s) 526, 559, 586, 756
BstMCI CGRYCG 2 cut(s) 717, 723
BstMWI GCNNNNNNNGC 2 cut(s) 209, 490
BstNI CCWGG 1 cut(s) 768
BstNSI RCATGY 1 cut(s) 23
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 1 cut(s) 766
BstSFI CTRYAG 1 cut(s) 185
BstSLI GKGCMC 3 cut(s) 84, 814, 830
BstUI CGCG 1 cut(s) 719
BstX2I RGATCY 3 cut(s) 559, 586, 756
BstYI RGATCY 3 cut(s) 559, 586, 756
BstZI CGGCCG 1 cut(s) 714
BsuRI GGCC 5 cut(s) 82, 314, 442, 716, 818
BtgI CCRYGG 2 cut(s) 717, 819
BtrI CACGTC 1 cut(s) 215
BtsCI GGATG 2 cut(s) 775, 790
BtsIMutI CAGTG 1 cut(s) 828
BveI ACCTGC 1 cut(s) 475
Cac8I GCNNGC 2 cut(s) 642, 826
CfoI GCGC 1 cut(s) 226
Cfr10I RCCGGY 1 cut(s) 640
Cfr13I GGNCC 4 cut(s) 52, 80, 81, 537
Cfr42I CCGCGG 1 cut(s) 720
CviAII CATG 4 cut(s) 20, 46, 336, 679
DpnI GATC 4 cut(s) 528, 561, 588, 758
DpnII GATC 4 cut(s) 526, 559, 586, 756
DraI TTTAAA 1 cut(s) 207
DraIII CACNNNGTG 1 cut(s) 137
EaeI YGGCCR 2 cut(s) 440, 714
EagI CGGCCG 1 cut(s) 714
Eam1104I CTCTTC 2 cut(s) 455, 606
EarI CTCTTC 2 cut(s) 455, 606
EciI GGCGGA 1 cut(s) 530
Ecl136II GAGCTC 1 cut(s) 625
EclXI CGGCCG 1 cut(s) 714
Eco147I AGGCCT 1 cut(s) 314
Eco24I GRGCYC 2 cut(s) 84, 627
Eco47I GGWCC 2 cut(s) 52, 537
Eco52I CGGCCG 1 cut(s) 714
Eco53kI GAGCTC 1 cut(s) 625
Eco72I CACGTG 1 cut(s) 137
Eco88I CYCGRG 3 cut(s) 274, 647, 779
Eco91I GGTNACC 1 cut(s) 770
EcoICRI GAGCTC 1 cut(s) 625
EcoO109I RGGNCCY 1 cut(s) 81
EcoO65I GGTNACC 1 cut(s) 770
EcoRII CCWGG 1 cut(s) 766
EcoT38I GRGCYC 2 cut(s) 84, 627
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 4 cut(s) 23, 49, 339, 682
FaiI YATR 8 cut(s) 21, 47, 58, 337, 504, 665, 680, 841
FaqI GGGAC 1 cut(s) 178
FatI CATG 4 cut(s) 19, 45, 335, 678
Fnu4HI GCNGC 2 cut(s) 513, 717
FokI GGATG 2 cut(s) 762, 797
FriOI GRGCYC 2 cut(s) 84, 627
Fsp4HI GCNGC 2 cut(s) 513, 717
FspBI CTAG 2 cut(s) 260, 762
GlaI GCGC 1 cut(s) 225
GluI GCNGC 2 cut(s) 513, 717
HaeIII GGCC 5 cut(s) 82, 314, 442, 716, 818
HapII CCGG 5 cut(s) 408, 620, 641, 701, 713
HhaI GCGC 1 cut(s) 226
Hin1II CATG 4 cut(s) 23, 49, 339, 682
Hin6I GCGC 1 cut(s) 224
HinP1I GCGC 1 cut(s) 224
HinfI GANTC 6 cut(s) 332, 411, 594, 616, 725, 736
HpaII CCGG 5 cut(s) 408, 620, 641, 701, 713
HphI GGTGA 2 cut(s) 406, 782
Hpy166II GTNNAC 3 cut(s) 131, 142, 828
Hpy188III TCNNGA 7 cut(s) 242, 276, 408, 563, 620, 647, 779
Hpy8I GTNNAC 3 cut(s) 131, 142, 828
Hpy99I CGWCG 3 cut(s) 431, 524, 569
HpyAV CCTTC 2 cut(s) 325, 491
HpyCH4III ACNGT 2 cut(s) 69, 832
HpyCH4IV ACGT 2 cut(s) 136, 214
HpyCH4V TGCA 3 cut(s) 23, 828, 843
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 490
HpySE526I ACGT 2 cut(s) 136, 214
Hsp92II CATG 4 cut(s) 23, 49, 339, 682
HspAI GCGC 1 cut(s) 224
Kpn2I TCCGGA 2 cut(s) 407, 619
KroI GCCGGC 1 cut(s) 640
KroNI GCCGGC 1 cut(s) 642
KspI CCGCGG 1 cut(s) 720
Kzo9I GATC 4 cut(s) 526, 559, 586, 756
LguI GCTCTTC 1 cut(s) 455
LmnI GCTCC 1 cut(s) 622
MaeI CTAG 2 cut(s) 260, 762
MaeII ACGT 2 cut(s) 136, 214
MaeIII GTNAC 3 cut(s) 629, 656, 770
MalI GATC 4 cut(s) 528, 561, 588, 758
MboI GATC 4 cut(s) 526, 559, 586, 756
MfeI CAATTG 1 cut(s) 380
MflI RGATCY 3 cut(s) 559, 586, 756
MhlI GDGCHC 5 cut(s) 84, 627, 690, 814, 830
MluCI AATT 3 cut(s) 380, 741, 836
MlyI GAGTC 2 cut(s) 341, 745
MmeI TCCRAC 1 cut(s) 731
MnlI CCTC 9 cut(s) 99, 188, 237, 304, 424, 502, 528, 550, 775
MroI TCCGGA 2 cut(s) 407, 619
MroNI GCCGGC 1 cut(s) 640
MroXI GAANNNNTTC 1 cut(s) 615
MseI TTAA 2 cut(s) 206, 573
MspA1I CMGCKG 2 cut(s) 190, 719
MspI CCGG 5 cut(s) 408, 620, 641, 701, 713
MspR9I CCNGG 1 cut(s) 768
MunI CAATTG 1 cut(s) 380
MvaI CCWGG 1 cut(s) 768
MvnI CGCG 1 cut(s) 719
MwoI GCNNNNNNNGC 2 cut(s) 209, 490
NaeI GCCGGC 1 cut(s) 642
NdeII GATC 4 cut(s) 526, 559, 586, 756
NgoMIV GCCGGC 1 cut(s) 640
NlaIII CATG 4 cut(s) 23, 49, 339, 682
NlaIV GGNNCC 3 cut(s) 54, 82, 83
NmuCI GTSAC 1 cut(s) 770
NspI RCATGY 1 cut(s) 23
NspV TTCGAA 1 cut(s) 388
PaeR7I CTCGAG 2 cut(s) 274, 647
PceI AGGCCT 1 cut(s) 314
PciSI GCTCTTC 1 cut(s) 455
PcsI WCGNNNNNNNCGW 2 cut(s) 133, 561
PdiI GCCGGC 1 cut(s) 642
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 4 cut(s) 411, 594, 616, 725
PkrI GCNGC 2 cut(s) 514, 718
PleI GAGTC 2 cut(s) 340, 744
PmaCI CACGTG 1 cut(s) 137
PmlI CACGTG 1 cut(s) 137
PpsI GAGTC 2 cut(s) 340, 744
Ppu21I YACGTR 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 627
Psp6I CCWGG 1 cut(s) 766
PspCI CACGTG 1 cut(s) 137
PspEI GGTNACC 1 cut(s) 770
PspGI CCWGG 1 cut(s) 766
PspN4I GGNNCC 3 cut(s) 54, 82, 83
PspOMI GGGCCC 1 cut(s) 80
PspPI GGNCC 4 cut(s) 52, 80, 81, 537
PsuI RGATCY 3 cut(s) 559, 586, 756
PvuII CAGCTG 1 cut(s) 190
SacI GAGCTC 1 cut(s) 627
SacII CCGCGG 1 cut(s) 720
SapI GCTCTTC 1 cut(s) 455
SaqAI TTAA 2 cut(s) 206, 573
SatI GCNGC 2 cut(s) 513, 717
Sau3AI GATC 4 cut(s) 526, 559, 586, 756
Sau96I GGNCC 4 cut(s) 52, 80, 81, 537
SchI GAGTC 2 cut(s) 341, 745
ScrFI CCNGG 1 cut(s) 768
SduI GDGCHC 5 cut(s) 84, 627, 690, 814, 830
SfcI CTRYAG 1 cut(s) 185
Sfr274I CTCGAG 2 cut(s) 274, 647
Sfr303I CCGCGG 1 cut(s) 720
SfuI TTCGAA 1 cut(s) 388
SgrBI CCGCGG 1 cut(s) 720
SinI GGWCC 2 cut(s) 52, 537
SlaI CTCGAG 2 cut(s) 274, 647
SmlI CTYRAG 2 cut(s) 274, 647
SmoI CTYRAG 2 cut(s) 274, 647
Sse9I AATT 3 cut(s) 380, 741, 836
SseBI AGGCCT 1 cut(s) 314
SsiI CCGC 4 cut(s) 512, 515, 717, 719
SspMI CTAG 2 cut(s) 260, 762
SstI GAGCTC 1 cut(s) 627
StuI AGGCCT 1 cut(s) 314
StyD4I CCNGG 1 cut(s) 766
TaaI ACNGT 2 cut(s) 69, 832
TaiI ACGT 2 cut(s) 139, 217
TaqI TCGA 7 cut(s) 275, 388, 533, 564, 648, 723, 734
TasI AATT 3 cut(s) 380, 741, 836
TauI GCSGC 2 cut(s) 515, 719
TfiI GAWTC 4 cut(s) 411, 594, 616, 725
Tru1I TTAA 2 cut(s) 206, 573
Tru9I TTAA 2 cut(s) 206, 573
TscAI CASTG 1 cut(s) 835
TseFI GTSAC 1 cut(s) 770
Tsp45I GTSAC 1 cut(s) 770
TspDTI ATGAA 2 cut(s) 369, 667
TspGWI ACGGA 1 cut(s) 566
TspRI CASTG 1 cut(s) 835
VneI GTGCAC 1 cut(s) 826
VpaK11BI GGWCC 2 cut(s) 52, 537
XceI RCATGY 1 cut(s) 23
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XhoI CTCGAG 2 cut(s) 274, 647
XmnI GAANNNNTTC 1 cut(s) 615
XspI CTAG 2 cut(s) 260, 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.