pycom04g03860

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0006360
Physical Location & Seq
Reverse (-)
3459290 .. 3465350
6061 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1338 bp
ATGGGAGAGATGGATTTGGAGGCAGTGGGTGATCTTGCGCTGCACATAATCCTCAGCAAGTTAGGAGCGGAAGACTCAGCGAGAGCAGCATGCGTCAGCACCAAACTCAGAGCTTCCGCCTCCGATGAATCCCTTTGGTCCCTTTTCTGTGCTCGAGACCTTCACCTCTTGCAACCCCTCGACCCCCTTGGCAACCTCGCCCCTTCTTTCAAGGAAAGTTACCAACTATGGCGTTTATCTTTTAAAATGTATCCTTGGTCGCTTGTAAAGCGAGTCAAAAGATGCTGGGACAAACTTAGAAACTGGTTGACTGTTAATTTTCCAGAGGCTGTTTCTACACTGAAAAAGGGTGCATCAGAAGCTGATATACAAGAGGTGGAGAAAATCTTGAAAGTGAAACTTCCTCTTCCAACAAGGATCCTCTATCGTTTTCATAATGGTCAAGCTTTTGAGGAAAAATATTTTCAAAATAGCCTGGTTGGCTGTCCATTGGGCCTCATAGGTGGTTATGCATTTTATGACCACTTGGTAACTGTGTATTTGATGCCTTTACGTCAGGTAGTCTTAGAAACAATGAAGATCAGACGTAAACTGGGTTTTACTGGTAGATCAGAATATGTTGTTGTGGCGGATTCTTGTACATATAGCAGGAAGTTATTTTTCCTGAACTGTACAAGTGGTCAACTCTATGTTGGTACCAGAAATCTTCCAACTGATGGGCAAATGCTTCCTTGCGTACCAGATGCGTTGATAAGTTCAATTCATGATTGCAATGTTGATCAACAGCAGGATGGCATGCTGCTTTGGTTAGAAGAGCATGGCCGTCGTTTAGAAAATGGCATTATTAAACTTCGTCAAGAAAATTTCAGAACCATCGGTCAGTTTCCAGAAGAATCCCCTCTATGTTCCACTGCTATAACGAATGGCATAAAGGTTCGTGCTTCTGCTGTGTTTGTCCCAGAGGCTGCTGATCTTGAAAATACTTCTGAAAAGTATACATTTTCTTATTCGATCCGCATGTCCCTTCTTCCTGAAGGATGTGTGATTAATGGAATGCCATTCAGCTCTTGTCAGTTGATTTGGAGGCACTGGATCATTCGTTCTAATGATGTTGTGGTAGCTGATGTTGGAGGAGAAGCTGTTATTGGACAGTACCCACTTTTGCGTCCAGGTGAAGAGGAATTCGTTTATGAGAGTTGCTCTTCTCTACCATCTTCTTCAGGTTCTATTGAAGGTTCTTTTACATTTGTCCCTGGCAGTTTGAGGGTGCCAAAGGGTGGCCCGTTTGAAGTTGCAGTGGCACGGTTTCCCCTCCAGCTGCCCAACTACATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF525 F-box SMI1_KNR4

Protein Analysis

446

Amino Acids

49.93

Weight (kDa)

6.42

Isoelectric Point (pI)

51.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF525 PF04379 326 - 417 1.4e-22 ApaG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 695
AccB1I GGYRCC 2 cut(s) 695, 1267
AccB7I CCANNNNNTGG 2 cut(s) 716, 1277
AccBSI CCGCTC 1 cut(s) 68
AccI GTMKAC 1 cut(s) 995
AciI CCGC 4 cut(s) 68, 117, 629, 1015
AclWI GGATC 4 cut(s) 412, 425, 1006, 1100
AcoI YGGCCR 1 cut(s) 820
AcsI RAATTY 2 cut(s) 862, 1181
AcuI CTGAAG 2 cut(s) 1053, 1203
AfaI GTAC 5 cut(s) 640, 673, 697, 738, 1154
AfiI CCNNNNNNNGG 2 cut(s) 716, 1277
AgsI TTSAA 7 cut(s) 211, 391, 467, 759, 977, 1232, 1289
AjnI CCWGG 3 cut(s) 474, 1168, 1252
AjuI GAANNNNNNNTTGG 3 cut(s) 1128, 1160, 1316
AloI GAACNNNNNNTCC 2 cut(s) 1084, 1116
AluBI AGCT 7 cut(s) 113, 362, 446, 1065, 1121, 1139, 1318
AluI AGCT 7 cut(s) 113, 362, 446, 1065, 1121, 1139, 1318
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 1 cut(s) 150
AlwI GGATC 4 cut(s) 412, 425, 1006, 1100
AlwNI CAGNNNCTG 3 cut(s) 329, 362, 965
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 3 cut(s) 493, 820, 1279
ApeKI GCWGC 5 cut(s) 40, 86, 799, 965, 1318
ApoI RAATTY 2 cut(s) 862, 1181
AseI ATTAAT 1 cut(s) 1047
Asp718I GGTACC 1 cut(s) 695
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 3 cut(s) 138, 493, 1280
AsuHPI GGTGA 3 cut(s) 41, 155, 1184
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 1 cut(s) 138
BamHI GGATCC 1 cut(s) 417
BanI GGYRCC 2 cut(s) 695, 1267
BarI GAAGNNNNNNTAC 2 cut(s) 351, 383
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 154
BbvCI CCTCAGC 1 cut(s) 53
BbvI GCAGC 5 cut(s) 27, 98, 786, 952, 1305
BccI CCATC 5 cut(s) 4, 710, 785, 881, 1219
BceAI ACGGC 1 cut(s) 807
BcgI CGANNNNNNTGC 2 cut(s) 806, 840
BciT130I CCWGG 3 cut(s) 476, 1170, 1254
BciVI GTATCC 1 cut(s) 261
BclI TGATCA 1 cut(s) 778
BcoDI GTCTC 1 cut(s) 150
BfuI GTATCC 1 cut(s) 261
BglI GCCNNNNNGGC 1 cut(s) 480
BisI GCNGC 5 cut(s) 41, 87, 800, 966, 1319
BlsI GCNGC 5 cut(s) 42, 88, 801, 967, 1320
Bme1390I CCNGG 3 cut(s) 476, 1170, 1254
Bme18I GGWCC 1 cut(s) 138
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 3 cut(s) 138, 493, 1280
BmiI GGNNCC 4 cut(s) 140, 419, 697, 1269
BmrFI CCNGG 3 cut(s) 476, 1170, 1254
BmrI ACTGGG 1 cut(s) 602
BmsI GCATC 4 cut(s) 272, 362, 534, 733
BmuI ACTGGG 1 cut(s) 602
BpiI GAAGAC 1 cut(s) 78
BplI GAGNNNNNCTC 2 cut(s) 1184, 1216
BpmI CTGGAG 1 cut(s) 1298
Bpu10I CCTNAGC 1 cut(s) 53
BsaI GGTCTC 1 cut(s) 150
BsaJI CCNNGG 3 cut(s) 187, 254, 1252
BsaXI ACNNNNNCTCC 2 cut(s) 1125, 1155
Bsc4I CCNNNNNNNGG 2 cut(s) 716, 1277
Bse1I ACTGG 4 cut(s) 308, 597, 607, 1094
Bse3DI GCAATG 1 cut(s) 778
BseBI CCWGG 3 cut(s) 476, 1170, 1254
BseDI CCNNGG 3 cut(s) 187, 254, 1252
BseGI GGATG 2 cut(s) 796, 1043
BseLI CCNNNNNNNGG 2 cut(s) 716, 1277
BseMI GCAATG 1 cut(s) 778
BseMII CTCAG 3 cut(s) 67, 90, 121
BseNI ACTGG 4 cut(s) 308, 597, 607, 1094
BseRI GAGGAG 1 cut(s) 1146
BseXI GCAGC 5 cut(s) 27, 98, 786, 952, 1305
BseYI CCCAGC 1 cut(s) 285
BsgI GTGCAG 1 cut(s) 26
BshFI GGCC 3 cut(s) 495, 822, 1281
BshNI GGYRCC 2 cut(s) 695, 1267
BsiHKAI GWGCWC 1 cut(s) 154
BsiHKCI CYCGRG 1 cut(s) 153
BslFI GGGAC 5 cut(s) 124, 302, 941, 1006, 1235
BslI CCNNNNNNNGG 2 cut(s) 716, 1277
BsmAI GTCTC 1 cut(s) 150
BsmFI GGGAC 5 cut(s) 124, 302, 941, 1006, 1235
BsmI GAATGC 1 cut(s) 1059
BsnI GGCC 3 cut(s) 495, 822, 1281
Bso31I GGTCTC 1 cut(s) 150
BsoBI CYCGRG 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 154
Bsp1407I TGTACA 2 cut(s) 638, 671
Bsp143I GATC 8 cut(s) 31, 417, 579, 608, 778, 970, 1011, 1092
BspACI CCGC 4 cut(s) 68, 117, 629, 1015
BspANI GGCC 3 cut(s) 495, 822, 1281
BspCNI CTCAG 3 cut(s) 66, 89, 120
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 4 cut(s) 140, 419, 697, 1269
BspPI GGATC 4 cut(s) 412, 425, 1006, 1100
BspQI GCTCTTC 2 cut(s) 807, 1207
BspT107I GGYRCC 2 cut(s) 695, 1267
BspTNI GGTCTC 1 cut(s) 150
BsrBI CCGCTC 1 cut(s) 68
BsrDI GCAATG 1 cut(s) 778
BsrGI TGTACA 2 cut(s) 638, 671
BsrI ACTGG 4 cut(s) 308, 597, 607, 1094
BssECI CCNNGG 3 cut(s) 187, 254, 1252
BssMI GATC 8 cut(s) 31, 417, 579, 608, 778, 970, 1011, 1092
BssNAI GTATAC 1 cut(s) 996
BssT1I CCWWGG 2 cut(s) 187, 254
Bst1107I GTATAC 1 cut(s) 996
Bst2UI CCWGG 3 cut(s) 476, 1170, 1254
Bst4CI ACNGT 5 cut(s) 313, 535, 671, 1152, 1305
Bst6I CTCTTC 4 cut(s) 411, 807, 1170, 1207
BstAUI TGTACA 2 cut(s) 638, 671
BstC8I GCNNGC 2 cut(s) 91, 797
BstDEI CTNAG 5 cut(s) 53, 76, 107, 296, 565
BstF5I GGATG 2 cut(s) 796, 1043
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 8 cut(s) 34, 420, 582, 611, 781, 973, 1014, 1095
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 8 cut(s) 31, 417, 579, 608, 778, 970, 1011, 1092
BstMWI GCNNNNNNNGC 4 cut(s) 86, 268, 359, 480
BstNI CCWGG 3 cut(s) 476, 1170, 1254
BstNSI RCATGY 3 cut(s) 93, 799, 1021
BstSCI CCNGG 3 cut(s) 474, 1168, 1252
BstV1I GCAGC 5 cut(s) 27, 98, 786, 952, 1305
BstV2I GAAGAC 1 cut(s) 78
BstX2I RGATCY 1 cut(s) 417
BstYI RGATCY 1 cut(s) 417
BstZ17I GTATAC 1 cut(s) 996
BsuI GTATCC 1 cut(s) 261
BsuRI GGCC 3 cut(s) 495, 822, 1281
BtsCI GGATG 2 cut(s) 796, 1043
BtsI GCAGTG 3 cut(s) 30, 909, 1302
BtsIMutI CAGTG 5 cut(s) 30, 338, 909, 1087, 1302
Cac8I GCNNGC 2 cut(s) 91, 797
CaiI CAGNNNCTG 3 cut(s) 329, 362, 965
CciI TCATGA 1 cut(s) 763
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 3 cut(s) 138, 493, 1280
CseI GACGC 2 cut(s) 82, 1154
Csp6I GTAC 5 cut(s) 639, 672, 696, 737, 1153
CviAII CATG 5 cut(s) 90, 764, 796, 818, 1018
CviQI GTAC 5 cut(s) 639, 672, 696, 737, 1153
DdeI CTNAG 5 cut(s) 53, 76, 107, 296, 565
DpnI GATC 8 cut(s) 33, 419, 581, 610, 780, 972, 1013, 1094
DpnII GATC 8 cut(s) 31, 417, 579, 608, 778, 970, 1011, 1092
DraI TTTAAA 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 820
Eam1104I CTCTTC 4 cut(s) 411, 807, 1170, 1207
EarI CTCTTC 4 cut(s) 411, 807, 1170, 1207
EciI GGCGGA 2 cut(s) 106, 644
Eco130I CCWWGG 2 cut(s) 187, 254
Eco31I GGTCTC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 138
Eco57I CTGAAG 2 cut(s) 1053, 1203
Eco88I CYCGRG 1 cut(s) 153
EcoRI GAATTC 1 cut(s) 1181
EcoRII CCWGG 3 cut(s) 474, 1168, 1252
EcoT14I CCWWGG 2 cut(s) 187, 254
EcoT22I ATGCAT 1 cut(s) 514
ErhI CCWWGG 2 cut(s) 187, 254
FaeI CATG 5 cut(s) 93, 767, 799, 821, 1021
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 5 cut(s) 124, 302, 941, 1006, 1235
FatI CATG 5 cut(s) 89, 763, 795, 817, 1017
FbaI TGATCA 1 cut(s) 778
FblI GTMKAC 1 cut(s) 995
Fnu4HI GCNGC 5 cut(s) 41, 87, 800, 966, 1319
FokI GGATG 2 cut(s) 803, 1050
Fsp4HI GCNGC 5 cut(s) 41, 87, 800, 966, 1319
GlaI GCGC 1 cut(s) 39
GluI GCNGC 5 cut(s) 41, 87, 800, 966, 1319
GsaI CCCAGC 1 cut(s) 289
GsuI CTGGAG 1 cut(s) 1298
HaeIII GGCC 3 cut(s) 495, 822, 1281
HgaI GACGC 2 cut(s) 82, 1154
HhaI GCGC 1 cut(s) 40
Hin1II CATG 5 cut(s) 93, 767, 799, 821, 1021
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 2 cut(s) 309, 683
HindII GTYRAC 2 cut(s) 309, 683
HindIII AAGCTT 1 cut(s) 444
HinfI GANTC 5 cut(s) 74, 128, 273, 632, 893
HphI GGTGA 3 cut(s) 41, 155, 1184
Hpy166II GTNNAC 4 cut(s) 309, 590, 683, 996
Hpy188I TCNGA 8 cut(s) 110, 124, 358, 584, 613, 869, 988, 1337
Hpy188III TCNNGA 9 cut(s) 155, 323, 388, 664, 764, 857, 887, 974, 1031
Hpy8I GTNNAC 4 cut(s) 309, 590, 683, 996
Hpy99I CGWCG 1 cut(s) 828
HpyAV CCTTC 5 cut(s) 170, 213, 1028, 1034, 1226
HpyCH4III ACNGT 5 cut(s) 313, 535, 671, 1152, 1305
HpyCH4IV ACGT 2 cut(s) 553, 586
HpyCH4V TGCA 6 cut(s) 43, 172, 353, 512, 771, 1295
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 268, 359, 480
HpyF3I CTNAG 5 cut(s) 53, 76, 107, 296, 565
HpySE526I ACGT 2 cut(s) 553, 586
Hsp92II CATG 5 cut(s) 93, 767, 799, 821, 1021
HspAI GCGC 1 cut(s) 38
KpnI GGTACC 1 cut(s) 699
Ksp22I TGATCA 1 cut(s) 778
Kzo9I GATC 8 cut(s) 31, 417, 579, 608, 778, 970, 1011, 1092
LguI GCTCTTC 2 cut(s) 807, 1207
LmnI GCTCC 1 cut(s) 65
Lsp1109I GCAGC 5 cut(s) 27, 98, 786, 952, 1305
LweI GCATC 4 cut(s) 272, 362, 534, 733
MaeII ACGT 2 cut(s) 553, 586
MaeIII GTNAC 2 cut(s) 218, 529
MalI GATC 8 cut(s) 33, 419, 581, 610, 780, 972, 1013, 1094
MbiI CCGCTC 1 cut(s) 68
MboI GATC 8 cut(s) 31, 417, 579, 608, 778, 970, 1011, 1092
MflI RGATCY 1 cut(s) 417
MhlI GDGCHC 1 cut(s) 154
MluCI AATT 4 cut(s) 316, 759, 862, 1181
MlyI GAGTC 2 cut(s) 68, 282
MmeI TCCRAC 3 cut(s) 434, 734, 1108
Mph1103I ATGCAT 1 cut(s) 514
MseI TTAA 4 cut(s) 243, 315, 846, 1047
MspA1I CMGCKG 1 cut(s) 1318
MspR9I CCNGG 3 cut(s) 476, 1170, 1254
Mva1269I GAATGC 1 cut(s) 1059
MvaI CCWGG 3 cut(s) 476, 1170, 1254
MwoI GCNNNNNNNGC 4 cut(s) 86, 268, 359, 480
NdeII GATC 8 cut(s) 31, 417, 579, 608, 778, 970, 1011, 1092
NlaIII CATG 5 cut(s) 93, 767, 799, 821, 1021
NlaIV GGNNCC 4 cut(s) 140, 419, 697, 1269
NsiI ATGCAT 1 cut(s) 514
NspI RCATGY 3 cut(s) 93, 799, 1021
PaeI GCATGC 2 cut(s) 93, 799
PaeR7I CTCGAG 1 cut(s) 153
PagI TCATGA 1 cut(s) 763
PciSI GCTCTTC 2 cut(s) 807, 1207
PctI GAATGC 1 cut(s) 1059
PfeI GAWTC 3 cut(s) 128, 632, 893
PflMI CCANNNNNTGG 2 cut(s) 716, 1277
PkrI GCNGC 5 cut(s) 42, 88, 801, 967, 1320
PleI GAGTC 2 cut(s) 68, 281
PpsI GAGTC 2 cut(s) 68, 281
PshBI ATTAAT 1 cut(s) 1047
Psp6I CCWGG 3 cut(s) 474, 1168, 1252
PspFI CCCAGC 1 cut(s) 285
PspGI CCWGG 3 cut(s) 474, 1168, 1252
PspN4I GGNNCC 4 cut(s) 140, 419, 697, 1269
PspPI GGNCC 3 cut(s) 138, 493, 1280
PstNI CAGNNNCTG 3 cut(s) 329, 362, 965
PsuI RGATCY 1 cut(s) 417
PvuII CAGCTG 1 cut(s) 1318
RsaI GTAC 5 cut(s) 640, 673, 697, 738, 1154
RsaNI GTAC 5 cut(s) 639, 672, 696, 737, 1153
SapI GCTCTTC 2 cut(s) 807, 1207
SaqAI TTAA 4 cut(s) 243, 315, 846, 1047
SatI GCNGC 5 cut(s) 41, 87, 800, 966, 1319
Sau3AI GATC 8 cut(s) 31, 417, 579, 608, 778, 970, 1011, 1092
Sau96I GGNCC 3 cut(s) 138, 493, 1280
SchI GAGTC 2 cut(s) 68, 282
ScrFI CCNGG 3 cut(s) 476, 1170, 1254
SduI GDGCHC 1 cut(s) 154
SfaNI GCATC 4 cut(s) 272, 362, 534, 733
Sfr274I CTCGAG 1 cut(s) 153
SinI GGWCC 1 cut(s) 138
SlaI CTCGAG 1 cut(s) 153
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
SphI GCATGC 2 cut(s) 93, 799
Sse9I AATT 4 cut(s) 316, 759, 862, 1181
SsiI CCGC 4 cut(s) 68, 117, 629, 1015
SspI AATATT 1 cut(s) 461
StyD4I CCNGG 3 cut(s) 474, 1168, 1252
StyI CCWWGG 2 cut(s) 187, 254
TaaI ACNGT 5 cut(s) 313, 535, 671, 1152, 1305
TaiI ACGT 2 cut(s) 556, 589
TaqI TCGA 3 cut(s) 154, 180, 1010
TaqII GACCGA 1 cut(s) 866
TasI AATT 4 cut(s) 316, 759, 862, 1181
TatI WGTACW 2 cut(s) 638, 671
TfiI GAWTC 3 cut(s) 128, 632, 893
Tru1I TTAA 4 cut(s) 243, 315, 846, 1047
Tru9I TTAA 4 cut(s) 243, 315, 846, 1047
TscAI CASTG 5 cut(s) 30, 345, 916, 1094, 1302
TseI GCWGC 5 cut(s) 40, 86, 799, 965, 1318
TspDTI ATGAA 4 cut(s) 141, 422, 590, 752
TspRI CASTG 5 cut(s) 30, 345, 916, 1094, 1302
Van91I CCANNNNNTGG 2 cut(s) 716, 1277
VpaK11BI GGWCC 1 cut(s) 138
VspI ATTAAT 1 cut(s) 1047
XapI RAATTY 2 cut(s) 862, 1181
XceI RCATGY 3 cut(s) 93, 799, 1021
XhoI CTCGAG 1 cut(s) 153
XmiI GTMKAC 1 cut(s) 995
Zsp2I ATGCAT 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.