Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0002992

Physical Location & Seq

Genomic Coordinates Forward (+)

6187772 .. 6197623

Gene Structure 9852 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4305 bp

ATGGACAGTGAATTGTATGAAGAAGAGCCAGTTCGATCTGGTATCATAACTGCCATACGTCTAAATGTTACAACTGGGGAAGATATTCTAAGGATATCTGAAGAAACACCAATCAGTTCAGCCAGCGAGGTCACTGATCCTAGGTTTGGACTTCCAAATCCATCTGGTCAGTGCTCTACTTGTGGTGCCGAAAACAAGTTACAGAACTGTGAAGGTCATTATGGGCGTATCCAGTTTCCATGCTCAATAGTCCATCCATATTATCTTCCAGAAGTTGTTCAAATCTTAAACAAGATTTGTGTAGGATGTAAATCTGTTAAGCAGGATCTAAAGCCCAAGGGTGCTGATTCTCTAAGTGGTAAGAGAAAGCGCCAGGAGCACAAGGAGCGCAAGGGCTGCAAGTATTGTAAGGGAAGTTCGTTAAGCTACCCAAGAATGAAGTTCAGAGTTTCTACGAGAGAACTCTTTAAAAGAAGTTGTATTATCGTGGAAGCAAGCGGAATATTGCCTTCTGACTTTTGGGATTTTATTCCCAAAGATGAACAGCAAGACGAGAGTTGCGTAAAACCAAACAAAAGAATTCTATCTCATGCGCAGGTTCATCATTTGTTGAAGGATGTTGATCCAAATTTCATTGCGAAGTTTGTCCCAATAACAGATTCACTTTCTATGGATTCTTTCCTTGTAACTCCAAATTGTCATCGCGTGACAGAATTTATGCATGCTTTTGCTAATGGGCAGCGGTTGATGTTTTTGAACTCAGAGAAACCAGGCAATGATTTGATTGCTGCTCAGCTAAAAAATGTCAAAGACGATCCTTCAAAATCATCTGGTTTGAGATGGATCAAAGATGTTTTTCTTGGGAAGCGAACTGATCACTGTTTTCGCACGGTGGTGGTTGGTGATCCAAACATAAAGCTCAGTGAAATTGGTATACCCTCAAATATTGCAGAGAGGATGCATATAGCGGAGAATCTGAACAGATGGAACTTAGAAAGGTTGGGTGCCACCTGCAATCTACTCCTAATGTTCAAACAAGTGATGCATGTTCGTAGAAAAGGTAGCATCGTTCTGGTACACCAGACAGACGAACTGCAGATGGGAGATGCTATTTACAGGCCTCTGAGTGATGGAGACATTGTATTGATAAATAGGCCTCCATCGATACATCAACACTCTCTCATTGCTTTATTTGTCAAGATACTTCCAGCAGCGCCTGTTGTATCTATAAACCCACTCTGTTGCTCTCCATTTCGTGGTGATTTTGATGGTGATTGCCTTCATGGGTATGTTCCTCAATCAGTTGATGCCAAAGTTGAGCTTAGGGAGCTTGTTGCTTTAGATAAACAATTGATTAATGGGCAGAGCGGTACCAACCTACTTTCACTTAGTCAGGATAGCTTAACGGCTGCCCATCTGATCCTGGAGGATGGTGTTCTCTTGAATCGTTCTCAAATGCAACAGTTGCAAAGGTTCTGTTGCAATGAACTGCCATCACCGGCAATCATTAAAGCTCCTTCACTTGATGGCCCTGCTTGGACTGGAAAGCAAGTTTTCAGCATGCTCCTACCTTTGGGTTTTGATTATGATTTTGCTCCAGATGGAGTTTGCGTTAGGAATGGGGAGCTGCTATCTTCTGAAGGTTCTTCATGGTTGCGAGATACGAGTGGAAATCTTTATCAGAGTCTCGTTAAACATTGCCAGGGTCCGGTACTTGACATCCTGCATGCTGCTCAGGAAGTTTTTTGTGAGTGGTTGTCAATGAGGGGCTTGACTGTCTCTCTCTCGGACTTGTACCTCGCTTCTGACTCATGCTCAAGGAATAACTTGATGGAGGAAATTTTCTGTGGGTTGCGAGAAGCAGAGCAGGCATGTATATTTAGACAGCTGTTGGTGGATAGTAGTCGGGAACTTCTTATGGGATGTGCTGAAGAGAATCAAAGTCCCGTGACTTCGGATGTCGACTACTTCTGTCACGAGAAGCAGAAATCTGCTGCCCTCAGTCAGGTTGTTCATGATGCTTTCAAGCAATTTTTTCGGGATATTCAAAATTTAGCTTACAAATATGCGGGTACGGACAACTCTTTGATGAATATGCTCAAGGCTGGAAGCAAGGGTAATATGCAGAAATTAGTGCAGCACAGTATGTGCCTTGGTTTGCAAAACTCACTAGTTCCATTATCATTCAGAATTCCCAACCAGCTCTCATGTGCTGCGTGGAATAATCAAAAGGCAATCAGTTCAGATCAGAAGGTTGAGGGAAGTTCTGAATGTGTCGAGTCCTACATCCCCTCTGCCGTGGTTGAAAACTCATTCTTGACAGGCTTAAATCCATTAGAATGTTTTATTCATTCAGTAACAAGTCGAGATAGTTCTTTTAGTGGAAATGCTGATCTTCCTGGGACTTTGACACGAAAGCTTATGTTTTTCATGCGGGATTTGTGCACTGCTTATGATGGAACGGTGAGAAATGCATATGGGAACAAGGTTGTTCAGTTCTCCTATGACATATCCTCTCCAGATAGCGCTAGTACCAGAGATGACGAGAGTATTACTGCTTGTGATGGTGTAGGTGGTCAACCTGTTGGCTCATTGTCTGCCTGTGCCATATCTGAAGCTGCATACAGTGCTCTAGATCAGCCAGTTAGTCTTCTCGAAACTTCTCCACTTCTAAATCTGAAGAATATCTTGGAATGTGGTTCAAAGAAAAGCAGTGCCAAGCAGACCATGTCTTTATATTTATCTGAGAAGCTTGGAAGACATAGGCACGGTTTCGAGTATGCAGCCTTGGAAGTTAAGAATTATTTGGAAGGGCTGAAATTTTCCGATATTGTTTCTACGGTCCTGATAATCTTCTCCTCCCAAAACGGGAGTAAAAAACGTTTCAGCCCATGGGTTACCCACTTTCACATAGGCAAGGAAATTGTGAGGAGGAGGAGACTGAAGATGCATTCCATTGTCAATTCCCTTTATGCTAGGTGCAACTCTGCCAGACCAGAACTAAAAGCTATTCTTCGCAGTTTGCTGATTACGAGCAAGGACTGTTCTGAAGCTCACACATGCAAGGGAGATAACGACACGTTTTGCATCACTGTTGCTATGGCTGAAAACCACAAAAGTTCTTCTGAACAGCTGGATAAAGCTCGAGACTTGGTGATACCTTTCCTGCTTGAAACAGTAGTTAAAGGGTTATTGGAGGTGAAAAAGGTGGATATTTTGTGGAATGACCAACCCAAACGATCAAAAGTAGCTGATGGTTCGTCCGGTGAACTTTACTTGAAGGTTTCTATGGTAGGAAAGAGCGGCAAGAAAAGACTTTGGAGTATGCTTTTGGATAGTTGTATCCAGATTATGAATGTGATTGATTGGTCACGAAGTCATCCCGATAATGTTCATGAGTATTGCCTTGCCTATGGAATTGATGTTGGGTGGAACTATTTCCTCAATAGTTTAGAGAGCACATGCAATGACATTGGAAAGAACATACTCCCCGAACATTTGATCCTTGTAGCAGATTGCCTCACATGCACAGGAGAATTTGTGGGCTTAAATGCCAAGGGCATTGCCCAACAACAAGAACACGCATCTGTATCATCGCCATTCATGCAAGCATGTTTCTCTACTCCCGGGGCTTGCTTTGTAAAAGCAGCCAAGGCTGGAGCCGTGGACGAACTTCCGGGGAGTTTGGAAGCCTTGGCATGGGGAAAGCCTCCTCCTTCTGGGACGGGCAGGCAATTTGAGGTTTTATTTTCTCAAAAGGGGCTCAACTTGTCAAAGCCACTTGATGTTTATGACATGCTTCGACGCCAAGTTGGTTCCATTTGTATTAAGGAGAATGAAGTTAAAATGCCTGAAGTTTTGAACAATTCACTCGACAAATGTGGTTCTCCATATCTGCTCCAAAATGCAAAATACGTTGGGCTTGAGAAGTTTGAGAGTATATCCAAATCAGTCATTAAAAAGTATCTCAAGTTCAATGATATCCTCAAGTGGTCTCAGACGTGGAGGCACATACTGCGCAAGTATCCAATCAATGAGACGTTAAATGATGAGGACAAGTCAAAATTGATGACGGCTTTATATTTTCATCCTCATAGAATGGATAAGATTGGACCTGGAGTTCAAGACATAAAGATTGGCGCTCACCCTAAACATGAGGGGGCACAATGCTTTATGGTGGTACGAACAGATGGAACTGTTGAAGATTTTTCGTACCACAAGTGCGTGATGGGTGCTCTTGAGATTGTAGCACCTCAGAGGGTCAAGAGCTACCAGGCAAAATGGTTTGGCCGCGGACCTGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000418 GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010495 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901698 GO:1901699 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1435

Amino Acids

159.61

Weight (kDa)

7.44

Isoelectric Point (pI)

46.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	39 - 208	9e-10	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	288 - 445	3.4e-32	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	450 - 598	1.2e-18	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	663 - 723	7.2e-09	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	775 - 1193	1e-08	RNA polymerase Rpb1, domain 5
DUF3223	PF11523	1336 - 1409	1.5e-26	Protein of unknown function (DUF3223)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002992)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63020 AT1G63020 AT1G63020 AT1G63020 AT1G63020
fragaria_vesca	FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400
malus_domestica	MD04G1068800.v1.1 MD06G1069300.v1.1 MD06G1069400.v1.1 MD06G1069500.v1.1 MD06G1069800.v1.1
prunus_persica	Prupe.5G089300_v2.0.a1
pyrus_communis	pycom04g06250
rosa_chinensis	RchiOBHm_Chr7g0199481
rosa_laevigata	RLG00000003790
rosa_multiflora	Rmu_co8411067.1_g000001 Rmu_sc0003890.1_g000001
rosa_roxburghii	Rroxscaffold_3G00256700
rosa_rugosa	Rorug07G0054300 Rorug07G0054400 Rorug07G0054500 Rorug07G0054500
rosa_samantha	Rh7AG179300 Rh7BG182800 Rh7CG188100 Rh7DG182200
rosa_wichuraiana	Rw7G015760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1019
Acc16I	TGCGCA	2 cut(s)	594, 4019
Acc36I	ACCTGC	2 cut(s)	586, 1019
Acc65I	GGTACC	1 cut(s)	1370
AccB1I	GGYRCC	3 cut(s)	185, 1004, 1370
AccB7I	CCANNNNNTGG	1 cut(s)	1256
AccBSI	CCGCTC	2 cut(s)	1368, 3303
AccI	GTMKAC	2 cut(s)	934, 1962
AccII	CGCG	2 cut(s)	705, 4293
AciI	CCGC	9 cut(s)	498, 742, 968, 1368, 2069, 2434, 3303, 4291, 4293
AclI	AACGTT	1 cut(s)	2881
AclWI	GGATC	8 cut(s)	131, 333, 617, 809, 851, 899, 1414, 3496
AcoI	YGGCCR	1 cut(s)	4288
AcsI	RAATTY	8 cut(s)	579, 628, 713, 1839, 2050, 2190, 2819, 3536
AcuI	CTGAAG	8 cut(s)	120, 1659, 1950, 2634, 2699, 2963, 3069, 3873
AcyI	GRCGYC	1 cut(s)	3805
AfaI	GTAC	8 cut(s)	1077, 1372, 1713, 1796, 2074, 2533, 4182, 4214
AfeI	AGCGCT	1 cut(s)	2527
AfiI	CCNNNNNNNGG	8 cut(s)	146, 1256, 1573, 1708, 2005, 2868, 3699, 3719
AflIII	ACRYGT	1 cut(s)	3078
AhlI	ACTAGT	1 cut(s)	2170
AjiI	CACGTC	1 cut(s)	4002
AjnI	CCWGG	7 cut(s)	372, 769, 1422, 1701, 2398, 4114, 4272
AjuI	GAANNNNNNNTTGG	4 cut(s)	643, 675, 2672, 2704
AleI	CACNNNNGTG	1 cut(s)	893
AloI	GAACNNNNNNTCC	4 cut(s)	3486, 3518, 4104, 4136
Alw21I	GWGCWC	6 cut(s)	176, 381, 2447, 2632, 3461, 4237
Alw26I	GTCTC	7 cut(s)	1128, 1691, 1783, 2932, 3141, 3999, 4031
Alw44I	GTGCAC	1 cut(s)	2443
AlwI	GGATC	8 cut(s)	131, 333, 617, 809, 851, 899, 1414, 3496
AlwNI	CAGNNNCTG	1 cut(s)	1217
Ama87I	CYCGRG	2 cut(s)	3144, 3626
Aor51HI	AGCGCT	1 cut(s)	2527
AoxI	GGCC	4 cut(s)	1118, 1154, 1528, 4288
ApaLI	GTGCAC	1 cut(s)	2443
ApoI	RAATTY	8 cut(s)	579, 628, 713, 1839, 2050, 2190, 2819, 3536
AseI	ATTAAT	1 cut(s)	1356
Asp700I	GAANNNNTTC	3 cut(s)	84, 276, 3437
Asp718I	GGTACC	1 cut(s)	1370
AspA2I	CCTAGG	1 cut(s)	140
AspLEI	GCGC	7 cut(s)	372, 390, 595, 1216, 2528, 4020, 4142
AspS9I	GGNCC	5 cut(s)	1529, 1706, 2842, 4112, 4295
AsuC2I	CCSGG	3 cut(s)	3627, 3628, 3678
AsuHPI	GGTGA	9 cut(s)	914, 1271, 1283, 1488, 2476, 3166, 3211, 3278, 4136
AvaI	CYCGRG	2 cut(s)	3144, 3626
AvaII	GGWCC	4 cut(s)	1706, 2842, 4112, 4295
AvrII	CCTAGG	1 cut(s)	140
BaeGI	GKGCMC	2 cut(s)	2447, 4165
BanI	GGYRCC	3 cut(s)	185, 1004, 1370
BanII	GRGCYC	1 cut(s)	3765
BauI	CACGAG	1 cut(s)	1976
BbsI	GAAGAC	2 cut(s)	2642, 2764
Bbv12I	GWGCWC	6 cut(s)	176, 381, 2447, 2632, 3461, 4237
BceAI	ACGGC	4 cut(s)	1422, 2282, 3647, 4089
BcgI	CGANNNNNNTGC	2 cut(s)	2018, 2052
BciT130I	CCWGG	7 cut(s)	374, 771, 1424, 1703, 2400, 4116, 4274
BciVI	GTATCC	3 cut(s)	239, 3353, 4035
BclI	TGATCA	1 cut(s)	874
BcnI	CCSGG	3 cut(s)	3627, 3628, 3678
BcoDI	GTCTC	7 cut(s)	1128, 1691, 1783, 2932, 3141, 3999, 4031
BcuI	ACTAGT	1 cut(s)	2170
BfaI	CTAG	5 cut(s)	141, 2171, 2529, 2633, 2976
BfmI	CTRYAG	1 cut(s)	1094
BfoI	RGCGCY	4 cut(s)	373, 1217, 2529, 4143
BfuAI	ACCTGC	2 cut(s)	586, 1019
BfuI	GTATCC	3 cut(s)	239, 3353, 4035
BlnI	CCTAGG	1 cut(s)	140
BlpI	GCTNAGC	1 cut(s)	792
Bme18I	GGWCC	4 cut(s)	1706, 2842, 4112, 4295
BmeT110I	CYCGRG	2 cut(s)	3144, 3626
BmgBI	CACGTC	1 cut(s)	4002
BmgT120I	GGNCC	5 cut(s)	1529, 1706, 2842, 4112, 4295
BmiI	GGNNCC	6 cut(s)	187, 1006, 1372, 1707, 3661, 3817
BmrI	ACTGGG	1 cut(s)	84
BmsI	GCATC	9 cut(s)	948, 1032, 1074, 1096, 1297, 2008, 2937, 3096, 3593
BmuI	ACTGGG	1 cut(s)	84
BpiI	GAAGAC	2 cut(s)	2642, 2764
BpmI	CTGGAG	5 cut(s)	1445, 1581, 2502, 3678, 4137
Bpu10I	CCTNAGC	2 cut(s)	1322, 1734
Bpu1102I	GCTNAGC	1 cut(s)	792
BpuEI	CTTGAG	6 cut(s)	1801, 2084, 3944, 3953, 3971, 4259
BpuMI	CCSGG	3 cut(s)	3627, 3628, 3678
Bsa29I	ATCGAT	1 cut(s)	1163
BsaBI	GATNNNNATC	2 cut(s)	310, 621
BsaHI	GRCGYC	1 cut(s)	3805
BsaI	GGTCTC	1 cut(s)	3999
BsaWI	WCCGGW	2 cut(s)	1708, 3263
BsaXI	ACNNNNNCTCC	6 cut(s)	1125, 1155, 1596, 1626, 3525, 3555
Bsc4I	CCNNNNNNNGG	8 cut(s)	146, 1256, 1573, 1708, 2005, 2868, 3699, 3719
Bse118I	RCCGGY	1 cut(s)	1498
Bse1I	ACTGG	5 cut(s)	29, 79, 232, 1546, 2642
Bse3DI	GCAATG	7 cut(s)	633, 781, 1182, 1489, 1696, 3472, 3561
Bse8I	GATNNNNATC	2 cut(s)	310, 621
BseBI	CCWGG	7 cut(s)	374, 771, 1424, 1703, 2400, 4116, 4274
BseCI	ATCGAT	1 cut(s)	1163
BseJI	GATNNNNATC	2 cut(s)	310, 621
BseLI	CCNNNNNNNGG	8 cut(s)	146, 1256, 1573, 1708, 2005, 2868, 3699, 3719
BseMI	GCAATG	7 cut(s)	633, 781, 1182, 1489, 1696, 3472, 3561
BseNI	ACTGG	5 cut(s)	29, 79, 232, 1546, 2642
BseRI	GAGGAG	5 cut(s)	2848, 2944, 2947, 2950, 3702
BseSI	GKGCMC	2 cut(s)	2447, 4165
BsgI	GTGCAG	1 cut(s)	2156
Bsh1236I	CGCG	2 cut(s)	705, 4293
BshFI	GGCC	4 cut(s)	1120, 1156, 1530, 4290
BshNI	GGYRCC	3 cut(s)	185, 1004, 1370
BshVI	ATCGAT	1 cut(s)	1163
BsiHKAI	GWGCWC	6 cut(s)	176, 381, 2447, 2632, 3461, 4237
BsiHKCI	CYCGRG	2 cut(s)	3144, 3626
BsiSI	CCGG	5 cut(s)	1499, 1709, 3264, 3627, 3677
BslFI	GGGAC	4 cut(s)	632, 1929, 2416, 3736
BslI	CCNNNNNNNGG	8 cut(s)	146, 1256, 1573, 1708, 2005, 2868, 3699, 3719
BsmAI	GTCTC	7 cut(s)	1128, 1691, 1783, 2932, 3141, 3999, 4031
BsmBI	CGTCTC	1 cut(s)	4031
BsmFI	GGGAC	4 cut(s)	632, 1929, 2416, 3736
BsmI	GAATGC	1 cut(s)	2950
BsnI	GGCC	4 cut(s)	1120, 1156, 1530, 4290
Bso31I	GGTCTC	1 cut(s)	3999
BsoBI	CYCGRG	2 cut(s)	3144, 3626
Bsp1286I	GDGCHC	8 cut(s)	176, 381, 2447, 2632, 3461, 3765, 4165, 4237
Bsp1720I	GCTNAGC	1 cut(s)	792
Bsp19I	CCATGG	1 cut(s)	2891
BspACI	CCGC	9 cut(s)	498, 742, 968, 1368, 2069, 2434, 3303, 4291, 4293
BspANI	GGCC	4 cut(s)	1120, 1156, 1530, 4290
BspDI	ATCGAT	1 cut(s)	1163
BspFNI	CGCG	2 cut(s)	705, 4293
BspHI	TCATGA	2 cut(s)	2014, 3394
BspLI	GGNNCC	6 cut(s)	187, 1006, 1372, 1707, 3661, 3817
BspMAI	CTGCAG	1 cut(s)	1098
BspMI	ACCTGC	2 cut(s)	586, 1019
BspPI	GGATC	8 cut(s)	131, 333, 617, 809, 851, 899, 1414, 3496
BspQI	GCTCTTC	1 cut(s)	18
BspT107I	GGYRCC	3 cut(s)	185, 1004, 1370
BspTNI	GGTCTC	1 cut(s)	3999
BsrBI	CCGCTC	2 cut(s)	1368, 3303
BsrDI	GCAATG	7 cut(s)	633, 781, 1182, 1489, 1696, 3472, 3561
BsrFI	RCCGGY	1 cut(s)	1498
BsrI	ACTGG	5 cut(s)	29, 79, 232, 1546, 2642
BssAI	RCCGGY	1 cut(s)	1498
BssNAI	GTATAC	1 cut(s)	935
BssNI	GRCGYC	1 cut(s)	3805
BssSI	CACGAG	1 cut(s)	1976
BssT1I	CCWWGG	8 cut(s)	140, 336, 2152, 2787, 2891, 3555, 3651, 3693
Bst1107I	GTATAC	1 cut(s)	935
Bst2BI	CACGAG	1 cut(s)	1976
Bst2UI	CCWGG	7 cut(s)	374, 771, 1424, 1703, 2400, 4116, 4274
Bst6I	CTCTTC	2 cut(s)	18, 1926
BstACI	GRCGYC	1 cut(s)	3805
BstAPI	GCANNNNNTGC	4 cut(s)	396, 1465, 2627, 4015
BstC8I	GCNNGC	9 cut(s)	124, 496, 723, 1562, 1728, 1869, 3609, 3634, 3731
BstDSI	CCRYGG	4 cut(s)	2298, 2891, 3663, 4291
BstEII	GGTNACC	1 cut(s)	2896
BstENI	CCTNNNNNAGG	1 cut(s)	2003
BstFNI	CGCG	2 cut(s)	705, 4293
BstH2I	RGCGCY	4 cut(s)	373, 1217, 2529, 4143
BstHHI	GCGC	7 cut(s)	372, 390, 595, 1216, 2528, 4020, 4142
BstMAI	GTCTC	7 cut(s)	1128, 1691, 1783, 2932, 3141, 3999, 4031
BstNI	CCWGG	7 cut(s)	374, 771, 1424, 1703, 2400, 4116, 4274
BstPI	GGTNACC	1 cut(s)	2896
BstSFI	CTRYAG	1 cut(s)	1094
BstSLI	GKGCMC	2 cut(s)	2447, 4165
BstUI	CGCG	2 cut(s)	705, 4293
BstV2I	GAAGAC	2 cut(s)	2642, 2764
BstX2I	RGATCY	1 cut(s)	325
BstYI	RGATCY	1 cut(s)	325
BstZ17I	GTATAC	1 cut(s)	935
Bsu15I	ATCGAT	1 cut(s)	1163
BsuI	GTATCC	3 cut(s)	239, 3353, 4035
BsuRI	GGCC	4 cut(s)	1120, 1156, 1530, 4290
BsuTUI	ATCGAT	1 cut(s)	1163
BtgI	CCRYGG	4 cut(s)	2298, 2891, 3663, 4291
BtgZI	GCGATG	2 cut(s)	686, 3579
BtrI	CACGTC	1 cut(s)	4002
BtsI	GCAGTG	2 cut(s)	2445, 2719
BtsIMutI	CAGTG	9 cut(s)	13, 132, 176, 877, 928, 2445, 2632, 2719, 3090
BveI	ACCTGC	2 cut(s)	586, 1019
Cac8I	GCNNGC	9 cut(s)	124, 496, 723, 1562, 1728, 1869, 3609, 3634, 3731
CaiI	CAGNNNCTG	1 cut(s)	1217
CciI	TCATGA	2 cut(s)	2014, 3394
CfoI	GCGC	7 cut(s)	372, 390, 595, 1216, 2528, 4020, 4142
Cfr10I	RCCGGY	1 cut(s)	1498
Cfr13I	GGNCC	5 cut(s)	1529, 1706, 2842, 4112, 4295
Cfr42I	CCGCGG	1 cut(s)	4294
Cfr9I	CCCGGG	1 cut(s)	3626
ClaI	ATCGAT	1 cut(s)	1163
CseI	GACGC	1 cut(s)	3813
Csp6I	GTAC	8 cut(s)	1076, 1371, 1712, 1795, 2073, 2532, 4181, 4213
CviQI	GTAC	8 cut(s)	1076, 1371, 1712, 1795, 2073, 2532, 4181, 4213
DraI	TTTAAA	1 cut(s)	469
EaeI	YGGCCR	1 cut(s)	4288
Eam1104I	CTCTTC	2 cut(s)	18, 1926
EarI	CTCTTC	2 cut(s)	18, 1926
Eco130I	CCWWGG	8 cut(s)	140, 336, 2152, 2787, 2891, 3555, 3651, 3693
Eco147I	AGGCCT	2 cut(s)	1120, 1156
Eco24I	GRGCYC	1 cut(s)	3765
Eco31I	GGTCTC	1 cut(s)	3999
Eco32I	GATATC	2 cut(s)	96, 3982
Eco47I	GGWCC	4 cut(s)	1706, 2842, 4112, 4295
Eco47III	AGCGCT	1 cut(s)	2527
Eco57I	CTGAAG	8 cut(s)	120, 1659, 1950, 2634, 2699, 2963, 3069, 3873
Eco88I	CYCGRG	2 cut(s)	3144, 3626
Eco91I	GGTNACC	1 cut(s)	2896
EcoNI	CCTNNNNNAGG	1 cut(s)	2003
EcoO65I	GGTNACC	1 cut(s)	2896
EcoRI	GAATTC	2 cut(s)	579, 2190
EcoRII	CCWGG	7 cut(s)	372, 769, 1422, 1701, 2398, 4114, 4272
EcoRV	GATATC	2 cut(s)	96, 3982
EcoT14I	CCWWGG	8 cut(s)	140, 336, 2152, 2787, 2891, 3555, 3651, 3693
EcoT22I	ATGCAT	5 cut(s)	723, 963, 1047, 2476, 2952
EcoT38I	GRGCYC	1 cut(s)	3765
ErhI	CCWWGG	8 cut(s)	140, 336, 2152, 2787, 2891, 3555, 3651, 3693
Esp3I	CGTCTC	1 cut(s)	4031
FalI	AAGNNNNNCTT	6 cut(s)	1305, 1337, 2672, 2704, 2997, 3029
FaqI	GGGAC	4 cut(s)	632, 1929, 2416, 3736
FauI	CCCGC	2 cut(s)	2062, 2427
FauNDI	CATATG	1 cut(s)	2476
FbaI	TGATCA	1 cut(s)	874
FblI	GTMKAC	2 cut(s)	934, 1962
FriOI	GRGCYC	1 cut(s)	3765
FspBI	CTAG	5 cut(s)	141, 2171, 2529, 2633, 2976
FspI	TGCGCA	2 cut(s)	594, 4019
GlaI	GCGC	7 cut(s)	371, 389, 594, 1215, 2527, 4019, 4141
GsuI	CTGGAG	5 cut(s)	1445, 1581, 2502, 3678, 4137
HaeII	RGCGCY	4 cut(s)	373, 1217, 2529, 4143
HaeIII	GGCC	4 cut(s)	1120, 1156, 1530, 4290
HapII	CCGG	5 cut(s)	1499, 1709, 3264, 3627, 3677
HgaI	GACGC	1 cut(s)	3813
HhaI	GCGC	7 cut(s)	372, 390, 595, 1216, 2528, 4020, 4142
Hin1I	GRCGYC	1 cut(s)	3805
Hin6I	GCGC	7 cut(s)	370, 388, 593, 1214, 2526, 4018, 4140
HinP1I	GCGC	7 cut(s)	370, 388, 593, 1214, 2526, 4018, 4140
HincII	GTYRAC	2 cut(s)	1963, 2579
HindII	GTYRAC	2 cut(s)	1963, 2579
HindIII	AAGCTT	2 cut(s)	2417, 2750
HinfI	GANTC	9 cut(s)	347, 659, 674, 973, 1444, 1684, 1808, 1936, 2279
HpaII	CCGG	5 cut(s)	1499, 1709, 3264, 3627, 3677
HphI	GGTGA	9 cut(s)	914, 1271, 1283, 1488, 2476, 3166, 3211, 3278, 4136
Hpy166II	GTNNAC	7 cut(s)	935, 1078, 1963, 2445, 2579, 3269, 3667
Hpy8I	GTNNAC	7 cut(s)	935, 1078, 1963, 2445, 2579, 3269, 3667
Hpy99I	CGWCG	1 cut(s)	3807
HpyCH4IV	ACGT	6 cut(s)	58, 2881, 3080, 3915, 4001, 4040
HpySE526I	ACGT	6 cut(s)	58, 2881, 3080, 3915, 4001, 4040
Hsp92I	GRCGYC	1 cut(s)	3805
HspAI	GCGC	7 cut(s)	370, 388, 593, 1214, 2526, 4018, 4140
KpnI	GGTACC	1 cut(s)	1374
Ksp22I	TGATCA	1 cut(s)	874
KspI	CCGCGG	1 cut(s)	4294
LguI	GCTCTTC	1 cut(s)	18
LmnI	GCTCC	9 cut(s)	376, 385, 1327, 1519, 1569, 1600, 1624, 3659, 3903
LweI	GCATC	9 cut(s)	948, 1032, 1074, 1096, 1297, 2008, 2937, 3096, 3593
MaeI	CTAG	5 cut(s)	141, 2171, 2529, 2633, 2976
MaeII	ACGT	6 cut(s)	58, 2881, 3080, 3915, 4001, 4040
MbiI	CCGCTC	2 cut(s)	1368, 3303
MfeI	CAATTG	1 cut(s)	1349
MflI	RGATCY	1 cut(s)	325
MhlI	GDGCHC	8 cut(s)	176, 381, 2447, 2632, 3461, 3765, 4165, 4237
MlyI	GAGTC	3 cut(s)	1693, 1802, 2288
Mph1103I	ATGCAT	5 cut(s)	723, 963, 1047, 2476, 2952
MroXI	GAANNNNTTC	3 cut(s)	84, 276, 3437
MslI	CAYNNNNRTG	3 cut(s)	893, 1287, 2338
MspA1I	CMGCKG	4 cut(s)	742, 1888, 3133, 4293
MspI	CCGG	5 cut(s)	1499, 1709, 3264, 3627, 3677
MunI	CAATTG	1 cut(s)	1349
Mva1269I	GAATGC	1 cut(s)	2950
MvaI	CCWGG	7 cut(s)	374, 771, 1424, 1703, 2400, 4116, 4274
MvnI	CGCG	2 cut(s)	705, 4293
NciI	CCSGG	3 cut(s)	3627, 3628, 3678
NcoI	CCATGG	1 cut(s)	2891
NdeI	CATATG	1 cut(s)	2476
NlaIV	GGNNCC	6 cut(s)	187, 1006, 1372, 1707, 3661, 3817
NmuCI	GTSAC	5 cut(s)	130, 706, 1948, 1973, 3369
NsbI	TGCGCA	2 cut(s)	594, 4019
NsiI	ATGCAT	5 cut(s)	723, 963, 1047, 2476, 2952
OliI	CACNNNNGTG	1 cut(s)	893
PaeI	GCATGC	3 cut(s)	725, 1564, 1730
PaeR7I	CTCGAG	1 cut(s)	3144
PagI	TCATGA	2 cut(s)	2014, 3394
PaqCI	CACCTGC	1 cut(s)	1019
PceI	AGGCCT	2 cut(s)	1120, 1156
PciSI	GCTCTTC	1 cut(s)	18
PcsI	WCGNNNNNNNCGW	1 cut(s)	558
PctI	GAATGC	1 cut(s)	2950
PdmI	GAANNNNTTC	3 cut(s)	84, 276, 3437
PfeI	GAWTC	6 cut(s)	347, 659, 674, 973, 1444, 1936
PflFI	GACNNNGTC	1 cut(s)	2728
PflMI	CCANNNNNTGG	1 cut(s)	1256
PfoI	TCCNGGA	1 cut(s)	1422
PleI	GAGTC	3 cut(s)	1692, 1802, 2287
PpsI	GAGTC	3 cut(s)	1692, 1802, 2287
PshBI	ATTAAT	1 cut(s)	1356
Psp1406I	AACGTT	1 cut(s)	2881
Psp6I	CCWGG	7 cut(s)	372, 769, 1422, 1701, 2398, 4114, 4272
PspEI	GGTNACC	1 cut(s)	2896
PspGI	CCWGG	7 cut(s)	372, 769, 1422, 1701, 2398, 4114, 4272
PspN4I	GGNNCC	6 cut(s)	187, 1006, 1372, 1707, 3661, 3817
PspPI	GGNCC	5 cut(s)	1529, 1706, 2842, 4112, 4295
PsrI	GAACNNNNNNTAC	4 cut(s)	400, 432, 3956, 3988
PstI	CTGCAG	1 cut(s)	1098
PstNI	CAGNNNCTG	1 cut(s)	1217
PsuI	RGATCY	1 cut(s)	325
PsyI	GACNNNGTC	1 cut(s)	2728
PvuII	CAGCTG	2 cut(s)	1888, 3133
RsaI	GTAC	8 cut(s)	1077, 1372, 1713, 1796, 2074, 2533, 4182, 4214
RsaNI	GTAC	8 cut(s)	1076, 1371, 1712, 1795, 2073, 2532, 4181, 4213
RseI	CAYNNNNRTG	3 cut(s)	893, 1287, 2338
SacII	CCGCGG	1 cut(s)	4294
SalI	GTCGAC	1 cut(s)	1961
SapI	GCTCTTC	1 cut(s)	18
Sau96I	GGNCC	5 cut(s)	1529, 1706, 2842, 4112, 4295
SchI	GAGTC	3 cut(s)	1693, 1802, 2288
SduI	GDGCHC	8 cut(s)	176, 381, 2447, 2632, 3461, 3765, 4165, 4237
SfaNI	GCATC	9 cut(s)	948, 1032, 1074, 1096, 1297, 2008, 2937, 3096, 3593
SfcI	CTRYAG	1 cut(s)	1094
Sfr274I	CTCGAG	1 cut(s)	3144
Sfr303I	CCGCGG	1 cut(s)	4294
SgrBI	CCGCGG	1 cut(s)	4294
SinI	GGWCC	4 cut(s)	1706, 2842, 4112, 4295
SlaI	CTCGAG	1 cut(s)	3144
SmaI	CCCGGG	1 cut(s)	3628
SmiMI	CAYNNNNRTG	3 cut(s)	893, 1287, 2338
SmlI	CTYRAG	7 cut(s)	1816, 2099, 3144, 3923, 3968, 3986, 4238
SmoI	CTYRAG	7 cut(s)	1816, 2099, 3144, 3923, 3968, 3986, 4238
SpeI	ACTAGT	1 cut(s)	2170
SphI	GCATGC	3 cut(s)	725, 1564, 1730
SseBI	AGGCCT	2 cut(s)	1120, 1156
SsiI	CCGC	9 cut(s)	498, 742, 968, 1368, 2069, 2434, 3303, 4291, 4293
SspI	AATATT	2 cut(s)	504, 946
SspMI	CTAG	5 cut(s)	141, 2171, 2529, 2633, 2976
StuI	AGGCCT	2 cut(s)	1120, 1156
StyI	CCWWGG	8 cut(s)	140, 336, 2152, 2787, 2891, 3555, 3651, 3693
TaiI	ACGT	6 cut(s)	61, 2884, 3083, 3918, 4004, 4043
TauI	GCSGC	2 cut(s)	3306, 4293
TfiI	GAWTC	6 cut(s)	347, 659, 674, 973, 1444, 1936
TscAI	CASTG	9 cut(s)	13, 139, 176, 884, 928, 2452, 2632, 2719, 3097
TseFI	GTSAC	5 cut(s)	130, 706, 1948, 1973, 3369
Tsp45I	GTSAC	5 cut(s)	130, 706, 1948, 1973, 3369
TspGWI	ACGGA	1 cut(s)	2090
TspMI	CCCGGG	1 cut(s)	3626
TspRI	CASTG	9 cut(s)	13, 139, 176, 884, 928, 2452, 2632, 2719, 3097
Tth111I	GACNNNGTC	1 cut(s)	2728
Van91I	CCANNNNNTGG	1 cut(s)	1256
VneI	GTGCAC	1 cut(s)	2443
VpaK11BI	GGWCC	4 cut(s)	1706, 2842, 4112, 4295
VspI	ATTAAT	1 cut(s)	1356
XagI	CCTNNNNNAGG	1 cut(s)	2003
XapI	RAATTY	8 cut(s)	579, 628, 713, 1839, 2050, 2190, 2819, 3536
XbaI	TCTAGA	1 cut(s)	2632
XhoI	CTCGAG	1 cut(s)	3144
XmaI	CCCGGG	1 cut(s)	3626
XmaJI	CCTAGG	1 cut(s)	140
XmiI	GTMKAC	2 cut(s)	934, 1962
XmnI	GAANNNNTTC	3 cut(s)	84, 276, 3437
XspI	CTAG	5 cut(s)	141, 2171, 2529, 2633, 2976
Zsp2I	ATGCAT	5 cut(s)	723, 963, 1047, 2476, 2952

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.