pycom04g11060

Glycine-rich RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0008734
Physical Location & Seq
Reverse (-)
13866848 .. 13868115
1268 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 426 bp
ATGGCGTTCATCTCCGCCTTCCGCCGCGTCCTCGGAGGATCTTCCTCCGCTCTCCATTCCCAATTCGCAGCCATTCGTCACAATTCAACTCTCACATCCCCCAGGCTGTTCATCAGCGGTCTCTCAAGATTAACAACAGACGAAAAGCTTAGAGAGGCATTTTCCCCATTTGGGCAGATTGTAGATGCGAAAGTGGTTATCGATAGGGTGTCTCAAAGATCGAAGGGTTTTGGTTTCATTACATACGCATCAGTTGAAGAAGCCGAGAAAGCTAGAGAAGGGATGAATGCCAAGTTCTTGGATGGGTGGGTTATTTTTGTCGACCCTGCCAAACCTAGGGAGCCTAGATTTCCTCCTCCGCAAGCACAGCAGGACCCTTCTGAAACCGGCTTCAGATCAAACAAGACCATCGGATGGTGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

142

Amino Acids

15.61

Weight (kDa)

9.79

Isoelectric Point (pI)

52.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 36 - 102 2.9e-20 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 414
AccBSI CCGCTC 1 cut(s) 50
AccI GTMKAC 1 cut(s) 321
AccII CGCG 1 cut(s) 27
AciI CCGC 6 cut(s) 15, 22, 25, 48, 117, 359
AclWI GGATC 1 cut(s) 46
AcuI CTGAAG 1 cut(s) 376
AfiI CCNNNNNNNGG 3 cut(s) 171, 336, 414
AgsI TTSAA 2 cut(s) 87, 257
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 2 cut(s) 148, 272
AluI AGCT 2 cut(s) 148, 272
Alw26I GTCTC 2 cut(s) 125, 216
AlwI GGATC 1 cut(s) 46
ApeKI GCWGC 1 cut(s) 68
AspA2I CCTAGG 1 cut(s) 335
AspS9I GGNCC 1 cut(s) 373
AvaII GGWCC 1 cut(s) 373
AvrII CCTAGG 1 cut(s) 335
BbvI GCAGC 1 cut(s) 80
BccI CCATC 3 cut(s) 296, 408, 416
BciT130I CCWGG 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 125, 216
BfaI CTAG 3 cut(s) 273, 336, 345
BisI GCNGC 2 cut(s) 25, 69
BlnI CCTAGG 1 cut(s) 335
BlsI GCNGC 2 cut(s) 26, 70
Bme1390I CCNGG 1 cut(s) 103
Bme18I GGWCC 1 cut(s) 373
BmgT120I GGNCC 1 cut(s) 373
BmiI GGNNCC 2 cut(s) 342, 375
BmrFI CCNGG 1 cut(s) 103
BmsI GCATC 2 cut(s) 175, 257
BpuEI CTTGAG 1 cut(s) 109
Bsa29I ATCGAT 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 3 cut(s) 31, 101, 335
Bsc4I CCNNNNNNNGG 3 cut(s) 171, 336, 414
Bse118I RCCGGY 1 cut(s) 386
BseBI CCWGG 1 cut(s) 103
BseCI ATCGAT 1 cut(s) 201
BseDI CCNNGG 3 cut(s) 31, 101, 335
BseGI GGATG 4 cut(s) 95, 288, 307, 419
BseLI CCNNNNNNNGG 3 cut(s) 171, 336, 414
BseRI GAGGAG 1 cut(s) 345
BseXI GCAGC 1 cut(s) 80
Bsh1236I CGCG 1 cut(s) 27
BshVI ATCGAT 1 cut(s) 201
BsiSI CCGG 1 cut(s) 387
BslI CCNNNNNNNGG 3 cut(s) 171, 336, 414
BsmAI GTCTC 2 cut(s) 125, 216
BsmI GAATGC 1 cut(s) 292
Bso31I GGTCTC 1 cut(s) 125
Bsp143I GATC 3 cut(s) 38, 218, 395
BspACI CCGC 6 cut(s) 15, 22, 25, 48, 117, 359
BspDI ATCGAT 1 cut(s) 201
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 2 cut(s) 342, 375
BspPI GGATC 1 cut(s) 46
BspTNI GGTCTC 1 cut(s) 125
BsrBI CCGCTC 1 cut(s) 50
BsrFI RCCGGY 1 cut(s) 386
BssAI RCCGGY 1 cut(s) 386
BssECI CCNNGG 3 cut(s) 31, 101, 335
BssMI GATC 3 cut(s) 38, 218, 395
BssT1I CCWWGG 1 cut(s) 335
Bst2UI CCWGG 1 cut(s) 103
BstC8I GCNNGC 1 cut(s) 363
BstDEI CTNAG 1 cut(s) 149
BstF5I GGATG 4 cut(s) 95, 288, 307, 419
BstFNI CGCG 1 cut(s) 27
BstKTI GATC 3 cut(s) 41, 221, 398
BstMAI GTCTC 2 cut(s) 125, 216
BstMBI GATC 3 cut(s) 38, 218, 395
BstMWI GCNNNNNNNGC 2 cut(s) 269, 367
BstNI CCWGG 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 101
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 80
BstX2I RGATCY 1 cut(s) 38
BstXI CCANNNNNNTGG 1 cut(s) 298
BstYI RGATCY 1 cut(s) 38
Bsu15I ATCGAT 1 cut(s) 201
BsuTUI ATCGAT 1 cut(s) 201
BtsCI GGATG 4 cut(s) 95, 288, 307, 419
Cac8I GCNNGC 1 cut(s) 363
Cfr10I RCCGGY 1 cut(s) 386
Cfr13I GGNCC 1 cut(s) 373
ClaI ATCGAT 1 cut(s) 201
CseI GACGC 1 cut(s) 16
CviJI RGCY 7 cut(s) 71, 106, 148, 263, 272, 343, 390
CviKI_1 RGCY 7 cut(s) 71, 106, 148, 263, 272, 343, 390
DdeI CTNAG 1 cut(s) 149
DpnI GATC 3 cut(s) 40, 220, 397
DpnII GATC 3 cut(s) 38, 218, 395
EciI GGCGGA 2 cut(s) 4, 11
Eco130I CCWWGG 1 cut(s) 335
Eco31I GGTCTC 1 cut(s) 125
Eco47I GGWCC 1 cut(s) 373
Eco57I CTGAAG 1 cut(s) 376
EcoO109I RGGNCCY 1 cut(s) 373
EcoRII CCWGG 1 cut(s) 101
EcoT14I CCWWGG 1 cut(s) 335
ErhI CCWWGG 1 cut(s) 335
FaiI YATR 1 cut(s) 244
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 2 cut(s) 25, 69
FokI GGATG 3 cut(s) 82, 295, 314
Fsp4HI GCNGC 2 cut(s) 25, 69
FspBI CTAG 3 cut(s) 273, 336, 345
GluI GCNGC 2 cut(s) 25, 69
HapII CCGG 1 cut(s) 387
HgaI GACGC 1 cut(s) 16
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 146
HpaII CCGG 1 cut(s) 387
Hpy166II GTNNAC 1 cut(s) 322
Hpy188I TCNGA 4 cut(s) 35, 382, 395, 413
Hpy188III TCNNGA 1 cut(s) 126
Hpy8I GTNNAC 1 cut(s) 322
HpyAV CCTTC 4 cut(s) 28, 217, 272, 387
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 367
HpyF3I CTNAG 1 cut(s) 149
Kzo9I GATC 3 cut(s) 38, 218, 395
LmnI GCTCC 1 cut(s) 340
LpnPI CCDG 5 cut(s) 88, 115, 339, 356, 400
Lsp1109I GCAGC 1 cut(s) 80
LweI GCATC 2 cut(s) 175, 257
MaeI CTAG 3 cut(s) 273, 336, 345
MaeIII GTNAC 1 cut(s) 77
MalI GATC 3 cut(s) 40, 220, 397
MbiI CCGCTC 1 cut(s) 50
MboI GATC 3 cut(s) 38, 218, 395
MboII GAAGA 2 cut(s) 33, 269
MflI RGATCY 1 cut(s) 38
MluCI AATT 2 cut(s) 62, 82
MnlI CCTC 6 cut(s) 29, 41, 55, 148, 363, 366
MseI TTAA 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 1 cut(s) 387
MspR9I CCNGG 1 cut(s) 103
Mva1269I GAATGC 1 cut(s) 292
MvaI CCWGG 1 cut(s) 103
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 2 cut(s) 269, 367
NdeII GATC 3 cut(s) 38, 218, 395
NlaIV GGNNCC 2 cut(s) 342, 375
NmeAIII GCCGAG 1 cut(s) 289
NmuCI GTSAC 1 cut(s) 77
PctI GAATGC 1 cut(s) 292
PflMI CCANNNNNTGG 1 cut(s) 414
PkrI GCNGC 2 cut(s) 26, 70
PpuMI RGGWCCY 1 cut(s) 373
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 2 cut(s) 342, 375
PspPI GGNCC 1 cut(s) 373
PspPPI RGGWCCY 1 cut(s) 373
PsuI RGATCY 1 cut(s) 38
SalI GTCGAC 1 cut(s) 320
SaqAI TTAA 1 cut(s) 131
SatI GCNGC 2 cut(s) 25, 69
Sau3AI GATC 3 cut(s) 38, 218, 395
Sau96I GGNCC 1 cut(s) 373
ScrFI CCNGG 1 cut(s) 103
SetI ASST 3 cut(s) 150, 274, 337
SfaNI GCATC 2 cut(s) 175, 257
SinI GGWCC 1 cut(s) 373
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
Sse9I AATT 2 cut(s) 62, 82
SsiI CCGC 6 cut(s) 15, 22, 25, 48, 117, 359
SspMI CTAG 3 cut(s) 273, 336, 345
StyD4I CCNGG 1 cut(s) 101
StyI CCWWGG 1 cut(s) 335
TaqI TCGA 3 cut(s) 201, 221, 321
TasI AATT 2 cut(s) 62, 82
TauI GCSGC 1 cut(s) 27
Tru1I TTAA 1 cut(s) 131
Tru9I TTAA 1 cut(s) 131
TseFI GTSAC 1 cut(s) 77
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 3 cut(s) 100, 226, 299
Van91I CCANNNNNTGG 1 cut(s) 414
VpaK11BI GGWCC 1 cut(s) 373
XmaJI CCTAGG 1 cut(s) 335
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 3 cut(s) 273, 336, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.