pycom04g14340

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002875
Physical Location & Seq
Forward (+)
17216987 .. 17217406
420 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g14340.1

Sequence Viewer

Length: 420 bp
ATGGCACTAATTTGGCCACTATCCAACAATCTCTACCTTGGCCAAATTGTATCAAAGTCCATTGGCCTTACGTACCAGCATGCACACTCTGAATCAACCTCGTTGGTCCTGTTCCGATTCAGTCACTGCCAATGCATGTGCAGCTGCTGCAAGCAGAAAAAACTTTTAGCTTCAGACAGGATCTCGACACATCCTCGTCTCCTCCAGCAGGTTTTCCTCCTCTTCATTGTCTGCAACTTGGAAACTTGTCAGTGCACCCATTTCCAGCAACACCCGTCGAGCCAGACAAACATTCCTCACACTTCCTCCTACCTCAGGACCTTCTCATCACATGTGAATCCCATAGCTCCACCTGCTGCAAAACCCCTGCAACACTTGAGACTAAACATCACCAGGAGAAGTGTTGCTTCGAGTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

140

Amino Acids

15.69

Weight (kDa)

9.5

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 361
Acc36I ACCTGC 2 cut(s) 199, 361
AclWI GGATC 1 cut(s) 188
AcoI YGGCCR 2 cut(s) 14, 40
AcuI CTGAAG 1 cut(s) 156
AfaI GTAC 2 cut(s) 74, 415
AfiI CCNNNNNNNGG 2 cut(s) 208, 315
AflIII ACRYGT 1 cut(s) 331
AjnI CCWGG 1 cut(s) 392
AluBI AGCT 3 cut(s) 144, 170, 347
AluI AGCT 3 cut(s) 144, 170, 347
Alw21I GWGCWC 1 cut(s) 257
Alw26I GTCTC 2 cut(s) 203, 373
Alw44I GTGCAC 1 cut(s) 253
AlwI GGATC 1 cut(s) 188
AlwNI CAGNNNCTG 2 cut(s) 126, 147
AoxI GGCC 3 cut(s) 14, 40, 64
ApaLI GTGCAC 1 cut(s) 253
ApeKI GCWGC 4 cut(s) 141, 144, 147, 356
AspS9I GGNCC 2 cut(s) 106, 318
AsuHPI GGTGA 1 cut(s) 382
AvaII GGWCC 2 cut(s) 106, 318
AxyI CCTNAGG 1 cut(s) 314
BaeGI GKGCMC 1 cut(s) 257
BalI TGGCCA 2 cut(s) 16, 42
Bbv12I GWGCWC 1 cut(s) 257
BbvI GCAGC 4 cut(s) 131, 134, 153, 343
BciT130I CCWGG 1 cut(s) 394
BcoDI GTCTC 2 cut(s) 203, 373
BfaI CTAG 1 cut(s) 418
BfuAI ACCTGC 2 cut(s) 199, 361
BisI GCNGC 4 cut(s) 142, 145, 148, 357
BlsI GCNGC 4 cut(s) 143, 146, 149, 358
Bme1390I CCNGG 1 cut(s) 394
Bme18I GGWCC 2 cut(s) 106, 318
BmgT120I GGNCC 2 cut(s) 106, 318
BmrFI CCNGG 1 cut(s) 394
BpmI CTGGAG 1 cut(s) 188
BpuEI CTTGAG 1 cut(s) 397
BsaAI YACGTR 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 37
BsaXI ACNNNNNCTCC 2 cut(s) 290, 320
Bsc4I CCNNNNNNNGG 2 cut(s) 208, 315
Bse21I CCTNAGG 1 cut(s) 314
BseBI CCWGG 1 cut(s) 394
BseDI CCNNGG 1 cut(s) 37
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 2 cut(s) 208, 315
BseMII CTCAG 1 cut(s) 328
BseRI GAGGAG 2 cut(s) 191, 209
BseSI GKGCMC 1 cut(s) 257
BseXI GCAGC 4 cut(s) 131, 134, 153, 343
BsgI GTGCAG 1 cut(s) 160
BshFI GGCC 3 cut(s) 16, 42, 66
BsiHKAI GWGCWC 1 cut(s) 257
BslI CCNNNNNNNGG 2 cut(s) 208, 315
BsmAI GTCTC 2 cut(s) 203, 373
BsmBI CGTCTC 1 cut(s) 203
BsnI GGCC 3 cut(s) 16, 42, 66
Bsp1286I GDGCHC 1 cut(s) 257
Bsp143I GATC 1 cut(s) 180
BspANI GGCC 3 cut(s) 16, 42, 66
BspCNI CTCAG 1 cut(s) 327
BspMI ACCTGC 2 cut(s) 199, 361
BspPI GGATC 1 cut(s) 188
BssECI CCNNGG 1 cut(s) 37
BssMI GATC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 37
Bst2UI CCWGG 1 cut(s) 394
Bst6I CTCTTC 1 cut(s) 227
BstAPI GCANNNNNTGC 1 cut(s) 147
BstBAI YACGTR 1 cut(s) 72
BstC8I GCNNGC 2 cut(s) 81, 152
BstDEI CTNAG 1 cut(s) 314
BstENI CCTNNNNNAGG 2 cut(s) 206, 313
BstF5I GGATG 1 cut(s) 190
BstKTI GATC 1 cut(s) 183
BstMAI GTCTC 2 cut(s) 203, 373
BstMBI GATC 1 cut(s) 180
BstMWI GCNNNNNNNGC 3 cut(s) 141, 147, 353
BstNI CCWGG 1 cut(s) 394
BstNSI RCATGY 3 cut(s) 83, 139, 335
BstSCI CCNGG 1 cut(s) 392
BstSLI GKGCMC 1 cut(s) 257
BstSNI TACGTA 1 cut(s) 72
BstV1I GCAGC 4 cut(s) 131, 134, 153, 343
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
Bsu36I CCTNAGG 1 cut(s) 314
BsuRI GGCC 3 cut(s) 16, 42, 66
BtsCI GGATG 1 cut(s) 190
BtsI GCAGTG 1 cut(s) 124
BtsIMutI CAGTG 2 cut(s) 124, 257
BveI ACCTGC 2 cut(s) 199, 361
Cac8I GCNNGC 2 cut(s) 81, 152
CaiI CAGNNNCTG 2 cut(s) 126, 147
Cfr13I GGNCC 2 cut(s) 106, 318
Csp6I GTAC 2 cut(s) 73, 414
CviAII CATG 3 cut(s) 80, 136, 332
CviJI RGCY 7 cut(s) 16, 42, 66, 144, 170, 282, 347
CviKI_1 RGCY 7 cut(s) 16, 42, 66, 144, 170, 282, 347
CviQI GTAC 2 cut(s) 73, 414
DdeI CTNAG 1 cut(s) 314
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
EaeI YGGCCR 2 cut(s) 14, 40
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Eco105I TACGTA 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 37
Eco47I GGWCC 2 cut(s) 106, 318
Eco57I CTGAAG 1 cut(s) 156
Eco81I CCTNAGG 1 cut(s) 314
EcoNI CCTNNNNNAGG 2 cut(s) 206, 313
EcoO109I RGGNCCY 1 cut(s) 318
EcoRII CCWGG 1 cut(s) 392
EcoT14I CCWWGG 1 cut(s) 37
EcoT22I ATGCAT 1 cut(s) 137
ErhI CCWWGG 1 cut(s) 37
Esp3I CGTCTC 1 cut(s) 203
FaeI CATG 3 cut(s) 83, 139, 335
FaiI YATR 4 cut(s) 81, 137, 333, 344
FalI AAGNNNNNCTT 1 cut(s) 391
FatI CATG 3 cut(s) 79, 135, 331
Fnu4HI GCNGC 4 cut(s) 142, 145, 148, 357
FokI GGATG 1 cut(s) 177
Fsp4HI GCNGC 4 cut(s) 142, 145, 148, 357
FspBI CTAG 1 cut(s) 418
GluI GCNGC 4 cut(s) 142, 145, 148, 357
GsuI CTGGAG 1 cut(s) 188
HaeIII GGCC 3 cut(s) 16, 42, 66
Hin1II CATG 3 cut(s) 83, 139, 335
HinfI GANTC 3 cut(s) 92, 117, 337
HphI GGTGA 1 cut(s) 382
Hpy166II GTNNAC 1 cut(s) 255
Hpy188I TCNGA 3 cut(s) 91, 116, 175
Hpy188III TCNNGA 2 cut(s) 184, 316
Hpy8I GTNNAC 1 cut(s) 255
Hpy99I CGWCG 1 cut(s) 280
HpyAV CCTTC 1 cut(s) 331
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 8 cut(s) 83, 135, 141, 150, 234, 255, 359, 370
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 147, 353
HpyF3I CTNAG 1 cut(s) 314
HpySE526I ACGT 1 cut(s) 71
Hsp92II CATG 3 cut(s) 83, 139, 335
Kzo9I GATC 1 cut(s) 180
LmnI GCTCC 1 cut(s) 352
Lsp1109I GCAGC 4 cut(s) 131, 134, 153, 343
MaeI CTAG 1 cut(s) 418
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 1 cut(s) 122
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 1 cut(s) 214
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 257
MlsI TGGCCA 2 cut(s) 16, 42
MluCI AATT 2 cut(s) 9, 45
MluNI TGGCCA 2 cut(s) 16, 42
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 8 cut(s) 109, 204, 212, 227, 230, 306, 316, 323
Mox20I TGGCCA 2 cut(s) 16, 42
Mph1103I ATGCAT 1 cut(s) 137
MscI TGGCCA 2 cut(s) 16, 42
Msp20I TGGCCA 2 cut(s) 16, 42
MspA1I CMGCKG 1 cut(s) 144
MspR9I CCNGG 1 cut(s) 394
MvaI CCWGG 1 cut(s) 394
MwoI GCNNNNNNNGC 3 cut(s) 141, 147, 353
NdeII GATC 1 cut(s) 180
NlaIII CATG 3 cut(s) 83, 139, 335
NmuCI GTSAC 1 cut(s) 122
NsiI ATGCAT 1 cut(s) 137
NspI RCATGY 3 cut(s) 83, 139, 335
PaeI GCATGC 1 cut(s) 83
PaqCI CACCTGC 1 cut(s) 361
PciI ACATGT 1 cut(s) 331
PfeI GAWTC 3 cut(s) 92, 117, 337
PkrI GCNGC 4 cut(s) 143, 146, 149, 358
Ppu21I YACGTR 1 cut(s) 72
PpuMI RGGWCCY 1 cut(s) 318
PscI ACATGT 1 cut(s) 331
Psp5II RGGWCCY 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 392
PspGI CCWGG 1 cut(s) 392
PspPI GGNCC 2 cut(s) 106, 318
PspPPI RGGWCCY 1 cut(s) 318
PstNI CAGNNNCTG 2 cut(s) 126, 147
PsuI RGATCY 1 cut(s) 180
PvuII CAGCTG 1 cut(s) 144
RsaI GTAC 2 cut(s) 74, 415
RsaNI GTAC 2 cut(s) 73, 414
SatI GCNGC 4 cut(s) 142, 145, 148, 357
Sau3AI GATC 1 cut(s) 180
Sau96I GGNCC 2 cut(s) 106, 318
ScrFI CCNGG 1 cut(s) 394
SduI GDGCHC 1 cut(s) 257
SinI GGWCC 2 cut(s) 106, 318
SmlI CTYRAG 1 cut(s) 376
SmoI CTYRAG 1 cut(s) 376
SnaBI TACGTA 1 cut(s) 72
SphI GCATGC 1 cut(s) 83
Sse9I AATT 2 cut(s) 9, 45
SspMI CTAG 1 cut(s) 418
StyD4I CCNGG 1 cut(s) 392
StyI CCWWGG 1 cut(s) 37
TaiI ACGT 1 cut(s) 74
TaqI TCGA 3 cut(s) 185, 278, 410
TasI AATT 2 cut(s) 9, 45
TfiI GAWTC 3 cut(s) 92, 117, 337
TscAI CASTG 2 cut(s) 131, 257
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 4 cut(s) 141, 144, 147, 356
Tsp45I GTSAC 1 cut(s) 122
TspDTI ATGAA 1 cut(s) 214
TspRI CASTG 2 cut(s) 131, 257
VneI GTGCAC 1 cut(s) 253
VpaK11BI GGWCC 2 cut(s) 106, 318
XagI CCTNNNNNAGG 2 cut(s) 206, 313
XceI RCATGY 3 cut(s) 83, 139, 335
XspI CTAG 1 cut(s) 418
Zsp2I ATGCAT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.