pycom10g26070

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002875
Physical Location & Seq
Reverse (-)
27493559 .. 27493900
342 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g26070.1

Sequence Viewer

Length: 342 bp
ATGCACACTCTGAATCAACCTCGTTGGTCCTGTTCCGATTCAGTCACTGCCAATGCATGTGCAGCTGCTGCAAGCAAAAAAAAACATTTAGCTTCAGACAGGATCTCGAAACATCCTCGTCTCCTCCAGCAGGTTTTCCTCCTCTTCATTGTCTGCAACTTGGAAAATTGTCAGTGCACCCATTTCCAACAACACCCGTTGAGCCAGACAAACATTCCTCACACTTCCTCCTACCTCAGGACCTTCTCATCACCTGTGAATCCCATAGCTCCACCTGCTGCAAAACCCCTGCAACACTTGAGACTAAACATCACTAAGAGAAGTGTTGCTTCGAGTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

114

Amino Acids

12.56

Weight (kDa)

9.98

Isoelectric Point (pI)

67.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 283
Acc36I ACCTGC 2 cut(s) 121, 283
AclWI GGATC 1 cut(s) 110
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 1 cut(s) 337
AfiI CCNNNNNNNGG 2 cut(s) 130, 237
AluBI AGCT 3 cut(s) 65, 92, 269
AluI AGCT 3 cut(s) 65, 92, 269
Alw21I GWGCWC 1 cut(s) 179
Alw26I GTCTC 2 cut(s) 125, 295
Alw44I GTGCAC 1 cut(s) 175
AlwI GGATC 1 cut(s) 110
AlwNI CAGNNNCTG 2 cut(s) 47, 68
ApaLI GTGCAC 1 cut(s) 175
ApeKI GCWGC 4 cut(s) 62, 65, 68, 278
AspS9I GGNCC 2 cut(s) 27, 240
AsuHPI GGTGA 1 cut(s) 243
AvaII GGWCC 2 cut(s) 27, 240
AxyI CCTNAGG 1 cut(s) 236
BaeGI GKGCMC 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 179
BbvI GCAGC 4 cut(s) 52, 55, 74, 265
BcoDI GTCTC 2 cut(s) 125, 295
BfaI CTAG 1 cut(s) 340
BfuAI ACCTGC 2 cut(s) 121, 283
BisI GCNGC 4 cut(s) 63, 66, 69, 279
BlsI GCNGC 4 cut(s) 64, 67, 70, 280
Bme18I GGWCC 2 cut(s) 27, 240
BmgT120I GGNCC 2 cut(s) 27, 240
BpmI CTGGAG 1 cut(s) 110
BpuEI CTTGAG 1 cut(s) 319
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 237
Bse21I CCTNAGG 1 cut(s) 236
BseGI GGATG 1 cut(s) 112
BseLI CCNNNNNNNGG 2 cut(s) 130, 237
BseMII CTCAG 1 cut(s) 250
BseRI GAGGAG 2 cut(s) 113, 131
BseSI GKGCMC 1 cut(s) 179
BseXI GCAGC 4 cut(s) 52, 55, 74, 265
BsgI GTGCAG 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 179
BslI CCNNNNNNNGG 2 cut(s) 130, 237
BsmAI GTCTC 2 cut(s) 125, 295
BsmBI CGTCTC 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 179
Bsp143I GATC 1 cut(s) 102
BspCNI CTCAG 1 cut(s) 249
BspMI ACCTGC 2 cut(s) 121, 283
BspPI GGATC 1 cut(s) 110
BssMI GATC 1 cut(s) 102
Bst6I CTCTTC 1 cut(s) 149
BstAPI GCANNNNNTGC 1 cut(s) 68
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 2 cut(s) 236, 315
BstENI CCTNNNNNAGG 2 cut(s) 128, 235
BstF5I GGATG 1 cut(s) 112
BstKTI GATC 1 cut(s) 105
BstMAI GTCTC 2 cut(s) 125, 295
BstMBI GATC 1 cut(s) 102
BstMWI GCNNNNNNNGC 3 cut(s) 62, 68, 275
BstNSI RCATGY 1 cut(s) 60
BstSLI GKGCMC 1 cut(s) 179
BstV1I GCAGC 4 cut(s) 52, 55, 74, 265
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
Bsu36I CCTNAGG 1 cut(s) 236
BtsCI GGATG 1 cut(s) 112
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 2 cut(s) 45, 179
BveI ACCTGC 2 cut(s) 121, 283
Cac8I GCNNGC 1 cut(s) 73
CaiI CAGNNNCTG 2 cut(s) 47, 68
Cfr13I GGNCC 2 cut(s) 27, 240
Csp6I GTAC 1 cut(s) 336
CviAII CATG 1 cut(s) 57
CviJI RGCY 4 cut(s) 65, 92, 204, 269
CviKI_1 RGCY 4 cut(s) 65, 92, 204, 269
CviQI GTAC 1 cut(s) 336
DdeI CTNAG 2 cut(s) 236, 315
DpnI GATC 1 cut(s) 104
DpnII GATC 1 cut(s) 102
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Eco47I GGWCC 2 cut(s) 27, 240
Eco57I CTGAAG 1 cut(s) 78
Eco81I CCTNAGG 1 cut(s) 236
EcoNI CCTNNNNNAGG 2 cut(s) 128, 235
EcoO109I RGGNCCY 1 cut(s) 240
EcoT22I ATGCAT 1 cut(s) 58
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 1 cut(s) 60
FaiI YATR 2 cut(s) 58, 266
FalI AAGNNNNNCTT 1 cut(s) 313
FatI CATG 1 cut(s) 56
Fnu4HI GCNGC 4 cut(s) 63, 66, 69, 279
FokI GGATG 1 cut(s) 99
Fsp4HI GCNGC 4 cut(s) 63, 66, 69, 279
FspBI CTAG 1 cut(s) 340
GluI GCNGC 4 cut(s) 63, 66, 69, 279
GsuI CTGGAG 1 cut(s) 110
Hin1II CATG 1 cut(s) 60
HinfI GANTC 3 cut(s) 13, 38, 259
HphI GGTGA 1 cut(s) 243
Hpy166II GTNNAC 1 cut(s) 177
Hpy188I TCNGA 3 cut(s) 12, 37, 97
Hpy188III TCNNGA 2 cut(s) 106, 238
Hpy8I GTNNAC 1 cut(s) 177
HpyAV CCTTC 1 cut(s) 253
HpyCH4V TGCA 8 cut(s) 4, 56, 62, 71, 156, 177, 281, 292
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 68, 275
HpyF3I CTNAG 2 cut(s) 236, 315
Hsp92II CATG 1 cut(s) 60
Kzo9I GATC 1 cut(s) 102
LmnI GCTCC 1 cut(s) 274
LpnPI CCDG 9 cut(s) 43, 85, 116, 140, 218, 223, 267, 288, 302
Lsp1109I GCAGC 4 cut(s) 52, 55, 74, 265
MaeI CTAG 1 cut(s) 340
MaeIII GTNAC 1 cut(s) 43
MalI GATC 1 cut(s) 104
MboI GATC 1 cut(s) 102
MboII GAAGA 1 cut(s) 136
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 1 cut(s) 179
MluCI AATT 1 cut(s) 166
MmeI TCCRAC 1 cut(s) 211
MnlI CCTC 8 cut(s) 30, 126, 134, 149, 152, 228, 238, 245
Mph1103I ATGCAT 1 cut(s) 58
MspA1I CMGCKG 1 cut(s) 65
MwoI GCNNNNNNNGC 3 cut(s) 62, 68, 275
NdeII GATC 1 cut(s) 102
NlaIII CATG 1 cut(s) 60
NmuCI GTSAC 1 cut(s) 43
NsiI ATGCAT 1 cut(s) 58
NspI RCATGY 1 cut(s) 60
PaqCI CACCTGC 1 cut(s) 283
PfeI GAWTC 3 cut(s) 13, 38, 259
PkrI GCNGC 4 cut(s) 64, 67, 70, 280
PpuMI RGGWCCY 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 240
PspPI GGNCC 2 cut(s) 27, 240
PspPPI RGGWCCY 1 cut(s) 240
PstNI CAGNNNCTG 2 cut(s) 47, 68
PsuI RGATCY 1 cut(s) 102
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
SatI GCNGC 4 cut(s) 63, 66, 69, 279
Sau3AI GATC 1 cut(s) 102
Sau96I GGNCC 2 cut(s) 27, 240
SduI GDGCHC 1 cut(s) 179
SinI GGWCC 2 cut(s) 27, 240
SmlI CTYRAG 1 cut(s) 298
SmoI CTYRAG 1 cut(s) 298
Sse9I AATT 1 cut(s) 166
SspMI CTAG 1 cut(s) 340
TaqI TCGA 2 cut(s) 107, 332
TasI AATT 1 cut(s) 166
TfiI GAWTC 3 cut(s) 13, 38, 259
TscAI CASTG 2 cut(s) 52, 179
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 4 cut(s) 62, 65, 68, 278
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 1 cut(s) 136
TspRI CASTG 2 cut(s) 52, 179
VneI GTGCAC 1 cut(s) 175
VpaK11BI GGWCC 2 cut(s) 27, 240
XagI CCTNNNNNAGG 2 cut(s) 128, 235
XceI RCATGY 1 cut(s) 60
XspI CTAG 1 cut(s) 340
Zsp2I ATGCAT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.