pycom13g04640

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002875
Physical Location & Seq
Forward (+)
3096714 .. 3097269
556 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g04640.1

Sequence Viewer

Length: 444 bp
ATGGCTTTGAAAAAGCCACAAAACAAGGAGCAAATTGGCCACTATCCAACAATCTCCACCTTGGCCAAATTGTACCAAAGTCCATTGGCCTTACGTACCAGCATGCACACTCTGAATCAACCTCGTTGGTCCTGTTCCGATTCAGTCACTGCCAATGCATGTGCAGCTGCTGCAAGCAAAAAAAAACATTTAGCTTCAGACAGGATCTCGACACATCCTCGTCTCCTCGAGCAGGTTTTCCTCCTCTTCATTGTCTGCAACTTGGAAACTTGTCAGTGCACCCATTTCCAACAACACCCGTCGAGCCAGACAAACATTCCTCACACTTCCTCCTACCTCAGGACCTTCTCATCACCTGTGAATCCCATAGCTCCACCTGCTGCAAAACCCCTGCAACACTTGAGACTAAACATCACCAGGAGAAGTGTTGCTTCGAGTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

148

Amino Acids

16.33

Weight (kDa)

9.98

Isoelectric Point (pI)

70.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 385
Acc36I ACCTGC 2 cut(s) 223, 385
AclWI GGATC 1 cut(s) 212
AcoI YGGCCR 2 cut(s) 37, 63
AcuI CTGAAG 1 cut(s) 180
AfaI GTAC 3 cut(s) 74, 97, 439
AfiI CCNNNNNNNGG 2 cut(s) 232, 339
AgsI TTSAA 1 cut(s) 10
AjnI CCWGG 1 cut(s) 416
AluBI AGCT 3 cut(s) 167, 194, 371
AluI AGCT 3 cut(s) 167, 194, 371
Alw21I GWGCWC 1 cut(s) 281
Alw26I GTCTC 2 cut(s) 227, 397
Alw44I GTGCAC 1 cut(s) 277
AlwI GGATC 1 cut(s) 212
AlwNI CAGNNNCTG 2 cut(s) 149, 170
Ama87I CYCGRG 1 cut(s) 227
AoxI GGCC 3 cut(s) 37, 63, 87
ApaLI GTGCAC 1 cut(s) 277
ApeKI GCWGC 4 cut(s) 164, 167, 170, 380
AspS9I GGNCC 2 cut(s) 129, 342
AsuHPI GGTGA 2 cut(s) 345, 406
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 2 cut(s) 129, 342
AxyI CCTNAGG 1 cut(s) 338
BaeGI GKGCMC 1 cut(s) 281
BalI TGGCCA 2 cut(s) 39, 65
Bbv12I GWGCWC 1 cut(s) 281
BbvI GCAGC 4 cut(s) 154, 157, 176, 367
BciT130I CCWGG 1 cut(s) 418
BcoDI GTCTC 2 cut(s) 227, 397
BfaI CTAG 1 cut(s) 442
BfuAI ACCTGC 2 cut(s) 223, 385
BisI GCNGC 4 cut(s) 165, 168, 171, 381
BlsI GCNGC 4 cut(s) 166, 169, 172, 382
Bme1390I CCNGG 1 cut(s) 418
Bme18I GGWCC 2 cut(s) 129, 342
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 2 cut(s) 129, 342
BmrFI CCNGG 1 cut(s) 418
BpuEI CTTGAG 1 cut(s) 421
BsaAI YACGTR 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 60
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 339
Bse21I CCTNAGG 1 cut(s) 338
BseBI CCWGG 1 cut(s) 418
BseDI CCNNGG 1 cut(s) 60
BseGI GGATG 1 cut(s) 214
BseLI CCNNNNNNNGG 2 cut(s) 232, 339
BseMII CTCAG 1 cut(s) 352
BseRI GAGGAG 2 cut(s) 215, 233
BseSI GKGCMC 1 cut(s) 281
BseXI GCAGC 4 cut(s) 154, 157, 176, 367
BsgI GTGCAG 1 cut(s) 183
BshFI GGCC 3 cut(s) 39, 65, 89
BsiHKAI GWGCWC 1 cut(s) 281
BsiHKCI CYCGRG 1 cut(s) 227
BslI CCNNNNNNNGG 2 cut(s) 232, 339
BsmAI GTCTC 2 cut(s) 227, 397
BsmBI CGTCTC 1 cut(s) 227
BsnI GGCC 3 cut(s) 39, 65, 89
BsoBI CYCGRG 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 281
Bsp143I GATC 1 cut(s) 204
BspANI GGCC 3 cut(s) 39, 65, 89
BspCNI CTCAG 1 cut(s) 351
BspMI ACCTGC 2 cut(s) 223, 385
BspPI GGATC 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 60
BssMI GATC 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 418
Bst6I CTCTTC 1 cut(s) 251
BstAPI GCANNNNNTGC 1 cut(s) 170
BstBAI YACGTR 1 cut(s) 95
BstC8I GCNNGC 2 cut(s) 104, 175
BstDEI CTNAG 1 cut(s) 338
BstENI CCTNNNNNAGG 2 cut(s) 230, 337
BstF5I GGATG 1 cut(s) 214
BstKTI GATC 1 cut(s) 207
BstMAI GTCTC 2 cut(s) 227, 397
BstMBI GATC 1 cut(s) 204
BstMWI GCNNNNNNNGC 3 cut(s) 164, 170, 377
BstNI CCWGG 1 cut(s) 418
BstNSI RCATGY 2 cut(s) 106, 162
BstSCI CCNGG 1 cut(s) 416
BstSLI GKGCMC 1 cut(s) 281
BstSNI TACGTA 1 cut(s) 95
BstV1I GCAGC 4 cut(s) 154, 157, 176, 367
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
Bsu36I CCTNAGG 1 cut(s) 338
BsuRI GGCC 3 cut(s) 39, 65, 89
BtsCI GGATG 1 cut(s) 214
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 147, 281
BveI ACCTGC 2 cut(s) 223, 385
Cac8I GCNNGC 2 cut(s) 104, 175
CaiI CAGNNNCTG 2 cut(s) 149, 170
Cfr13I GGNCC 2 cut(s) 129, 342
Csp6I GTAC 3 cut(s) 73, 96, 438
CviAII CATG 2 cut(s) 103, 159
CviJI RGCY 9 cut(s) 5, 16, 39, 65, 89, 167, 194, 306, 371
CviKI_1 RGCY 9 cut(s) 5, 16, 39, 65, 89, 167, 194, 306, 371
CviQI GTAC 3 cut(s) 73, 96, 438
DdeI CTNAG 1 cut(s) 338
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
EaeI YGGCCR 2 cut(s) 37, 63
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
Eco105I TACGTA 1 cut(s) 95
Eco130I CCWWGG 1 cut(s) 60
Eco47I GGWCC 2 cut(s) 129, 342
Eco57I CTGAAG 1 cut(s) 180
Eco81I CCTNAGG 1 cut(s) 338
Eco88I CYCGRG 1 cut(s) 227
EcoNI CCTNNNNNAGG 2 cut(s) 230, 337
EcoO109I RGGNCCY 1 cut(s) 342
EcoRII CCWGG 1 cut(s) 416
EcoT14I CCWWGG 1 cut(s) 60
EcoT22I ATGCAT 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 60
Esp3I CGTCTC 1 cut(s) 227
FaeI CATG 2 cut(s) 106, 162
FaiI YATR 3 cut(s) 104, 160, 368
FalI AAGNNNNNCTT 1 cut(s) 415
FatI CATG 2 cut(s) 102, 158
Fnu4HI GCNGC 4 cut(s) 165, 168, 171, 381
FokI GGATG 1 cut(s) 201
Fsp4HI GCNGC 4 cut(s) 165, 168, 171, 381
FspBI CTAG 1 cut(s) 442
GluI GCNGC 4 cut(s) 165, 168, 171, 381
HaeIII GGCC 3 cut(s) 39, 65, 89
Hin1II CATG 2 cut(s) 106, 162
HinfI GANTC 3 cut(s) 115, 140, 361
HphI GGTGA 2 cut(s) 345, 406
Hpy166II GTNNAC 1 cut(s) 279
Hpy188I TCNGA 3 cut(s) 114, 139, 199
Hpy188III TCNNGA 2 cut(s) 208, 340
Hpy8I GTNNAC 1 cut(s) 279
Hpy99I CGWCG 1 cut(s) 304
HpyAV CCTTC 1 cut(s) 355
HpyCH4IV ACGT 1 cut(s) 94
HpyCH4V TGCA 8 cut(s) 106, 158, 164, 173, 258, 279, 383, 394
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 170, 377
HpyF3I CTNAG 1 cut(s) 338
HpySE526I ACGT 1 cut(s) 94
Hsp92II CATG 2 cut(s) 106, 162
Kzo9I GATC 1 cut(s) 204
LmnI GCTCC 2 cut(s) 28, 376
Lsp1109I GCAGC 4 cut(s) 154, 157, 176, 367
MaeI CTAG 1 cut(s) 442
MaeII ACGT 1 cut(s) 94
MaeIII GTNAC 1 cut(s) 145
MalI GATC 1 cut(s) 206
MboI GATC 1 cut(s) 204
MboII GAAGA 1 cut(s) 238
MflI RGATCY 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 281
MlsI TGGCCA 2 cut(s) 39, 65
MluCI AATT 2 cut(s) 33, 68
MluNI TGGCCA 2 cut(s) 39, 65
MmeI TCCRAC 2 cut(s) 71, 313
MnlI CCTC 8 cut(s) 132, 228, 236, 251, 254, 330, 340, 347
Mox20I TGGCCA 2 cut(s) 39, 65
Mph1103I ATGCAT 1 cut(s) 160
MscI TGGCCA 2 cut(s) 39, 65
Msp20I TGGCCA 2 cut(s) 39, 65
MspA1I CMGCKG 1 cut(s) 167
MspR9I CCNGG 1 cut(s) 418
MvaI CCWGG 1 cut(s) 418
MwoI GCNNNNNNNGC 3 cut(s) 164, 170, 377
NdeII GATC 1 cut(s) 204
NlaIII CATG 2 cut(s) 106, 162
NmuCI GTSAC 1 cut(s) 145
NsiI ATGCAT 1 cut(s) 160
NspI RCATGY 2 cut(s) 106, 162
PaeI GCATGC 1 cut(s) 106
PaeR7I CTCGAG 1 cut(s) 227
PaqCI CACCTGC 1 cut(s) 385
PfeI GAWTC 3 cut(s) 115, 140, 361
PkrI GCNGC 4 cut(s) 166, 169, 172, 382
Ppu21I YACGTR 1 cut(s) 95
PpuMI RGGWCCY 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 416
PspGI CCWGG 1 cut(s) 416
PspPI GGNCC 2 cut(s) 129, 342
PspPPI RGGWCCY 1 cut(s) 342
PspXI VCTCGAGB 1 cut(s) 227
PstNI CAGNNNCTG 2 cut(s) 149, 170
PsuI RGATCY 1 cut(s) 204
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 3 cut(s) 74, 97, 439
RsaNI GTAC 3 cut(s) 73, 96, 438
SatI GCNGC 4 cut(s) 165, 168, 171, 381
Sau3AI GATC 1 cut(s) 204
Sau96I GGNCC 2 cut(s) 129, 342
ScrFI CCNGG 1 cut(s) 418
SduI GDGCHC 1 cut(s) 281
Sfr274I CTCGAG 1 cut(s) 227
SinI GGWCC 2 cut(s) 129, 342
SlaI CTCGAG 1 cut(s) 227
SmlI CTYRAG 2 cut(s) 227, 400
SmoI CTYRAG 2 cut(s) 227, 400
SnaBI TACGTA 1 cut(s) 95
SphI GCATGC 1 cut(s) 106
Sse9I AATT 2 cut(s) 33, 68
SspMI CTAG 1 cut(s) 442
StyD4I CCNGG 1 cut(s) 416
StyI CCWWGG 1 cut(s) 60
TaiI ACGT 1 cut(s) 97
TaqI TCGA 4 cut(s) 209, 228, 302, 434
TasI AATT 2 cut(s) 33, 68
TfiI GAWTC 3 cut(s) 115, 140, 361
TscAI CASTG 2 cut(s) 154, 281
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 4 cut(s) 164, 167, 170, 380
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 1 cut(s) 238
TspRI CASTG 2 cut(s) 154, 281
VneI GTGCAC 1 cut(s) 277
VpaK11BI GGWCC 2 cut(s) 129, 342
XagI CCTNNNNNAGG 2 cut(s) 230, 337
XceI RCATGY 2 cut(s) 106, 162
XhoI CTCGAG 1 cut(s) 227
XspI CTAG 1 cut(s) 442
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.