pycom12g19100

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002875
Physical Location & Seq
Reverse (-)
21049972 .. 21050484
513 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g19100.1

Sequence Viewer

Length: 426 bp
ATGGTGCCCTTCCTTCTTTTTTCTCATCTCTCGGCTGAAAGCAACAATGATTTTGTCCACCTTATTATTTTATTAGCCGAAAGTGGATGTGAGTTGGCCACTTGGCCACATATTCCAACAATCTCCACCTTGGCCAAGTTCCAAAATCCATTGGCCTTACGTACCAGCATGCACACTCTGAATCAACCTCGTTGGTCCTGTTCCGATTCAGTCACTGCCAATGCATGTGCAGCTGCTGCAAGCAAAAAAAAACATTTAGCTTCAGACAGGATCTCGACACATCCTCGTCTCCTCCAGCAGGTTTTCCTCCTCTTCATTGTCTGCAACTTGGAAACTTGTCAGTGCACCCATTTCCAGCAACACCCGTCGAGCCAGACAAACATTCCTCACACTTCTCATTCTTCAGGACCATCTCATCACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

142

Amino Acids

15.62

Weight (kDa)

7.76

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 289
AccB1I GGYRCC 1 cut(s) 4
AclWI GGATC 1 cut(s) 278
AcoI YGGCCR 3 cut(s) 96, 104, 132
AcuI CTGAAG 2 cut(s) 246, 387
AfaI GTAC 1 cut(s) 163
AfiI CCNNNNNNNGG 1 cut(s) 298
AluBI AGCT 2 cut(s) 233, 260
AluI AGCT 2 cut(s) 233, 260
Alw21I GWGCWC 1 cut(s) 347
Alw26I GTCTC 1 cut(s) 293
Alw44I GTGCAC 1 cut(s) 343
AlwI GGATC 1 cut(s) 278
AlwNI CAGNNNCTG 2 cut(s) 215, 236
AoxI GGCC 4 cut(s) 96, 104, 132, 153
ApaLI GTGCAC 1 cut(s) 343
ApeKI GCWGC 3 cut(s) 230, 233, 236
AspS9I GGNCC 2 cut(s) 195, 407
AvaII GGWCC 2 cut(s) 195, 407
BaeGI GKGCMC 2 cut(s) 9, 347
BalI TGGCCA 3 cut(s) 98, 106, 134
BanI GGYRCC 1 cut(s) 4
Bbv12I GWGCWC 1 cut(s) 347
BbvI GCAGC 3 cut(s) 220, 223, 242
BccI CCATC 1 cut(s) 418
BcoDI GTCTC 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 289
BisI GCNGC 3 cut(s) 231, 234, 237
BlsI GCNGC 3 cut(s) 232, 235, 238
Bme18I GGWCC 2 cut(s) 195, 407
BmgT120I GGNCC 2 cut(s) 195, 407
BmiI GGNNCC 1 cut(s) 6
BpmI CTGGAG 1 cut(s) 278
BsaAI YACGTR 1 cut(s) 161
BsaJI CCNNGG 1 cut(s) 129
Bsc4I CCNNNNNNNGG 1 cut(s) 298
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 2 cut(s) 92, 280
BseLI CCNNNNNNNGG 1 cut(s) 298
BseRI GAGGAG 2 cut(s) 281, 299
BseSI GKGCMC 2 cut(s) 9, 347
BseXI GCAGC 3 cut(s) 220, 223, 242
BsgI GTGCAG 1 cut(s) 249
BshFI GGCC 4 cut(s) 98, 106, 134, 155
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 347
BslI CCNNNNNNNGG 1 cut(s) 298
BsmAI GTCTC 1 cut(s) 293
BsmBI CGTCTC 1 cut(s) 293
BsnI GGCC 4 cut(s) 98, 106, 134, 155
Bsp1286I GDGCHC 2 cut(s) 9, 347
Bsp143I GATC 1 cut(s) 270
BspANI GGCC 4 cut(s) 98, 106, 134, 155
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 289
BspPI GGATC 1 cut(s) 278
BspT107I GGYRCC 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 1 cut(s) 270
BssT1I CCWWGG 1 cut(s) 129
Bst6I CTCTTC 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 236
BstBAI YACGTR 1 cut(s) 161
BstC8I GCNNGC 2 cut(s) 170, 241
BstENI CCTNNNNNAGG 1 cut(s) 296
BstF5I GGATG 2 cut(s) 92, 280
BstKTI GATC 1 cut(s) 273
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 1 cut(s) 270
BstMWI GCNNNNNNNGC 2 cut(s) 230, 236
BstNSI RCATGY 2 cut(s) 172, 228
BstSLI GKGCMC 2 cut(s) 9, 347
BstSNI TACGTA 1 cut(s) 161
BstV1I GCAGC 3 cut(s) 220, 223, 242
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
BsuRI GGCC 4 cut(s) 98, 106, 134, 155
BtsCI GGATG 2 cut(s) 92, 280
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 2 cut(s) 213, 347
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 2 cut(s) 170, 241
CaiI CAGNNNCTG 2 cut(s) 215, 236
Cfr13I GGNCC 2 cut(s) 195, 407
Csp6I GTAC 1 cut(s) 162
CviAII CATG 2 cut(s) 169, 225
CviJI RGCY 9 cut(s) 35, 77, 98, 106, 134, 155, 233, 260, 372
CviKI_1 RGCY 9 cut(s) 35, 77, 98, 106, 134, 155, 233, 260, 372
CviQI GTAC 1 cut(s) 162
DpnI GATC 1 cut(s) 272
DpnII GATC 1 cut(s) 270
EaeI YGGCCR 3 cut(s) 96, 104, 132
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
Eco105I TACGTA 1 cut(s) 161
Eco130I CCWWGG 1 cut(s) 129
Eco47I GGWCC 2 cut(s) 195, 407
Eco57I CTGAAG 2 cut(s) 246, 387
EcoNI CCTNNNNNAGG 1 cut(s) 296
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 1 cut(s) 226
ErhI CCWWGG 1 cut(s) 129
Esp3I CGTCTC 1 cut(s) 293
FaeI CATG 2 cut(s) 172, 228
FaiI YATR 3 cut(s) 111, 170, 226
FatI CATG 2 cut(s) 168, 224
Fnu4HI GCNGC 3 cut(s) 231, 234, 237
FokI GGATG 2 cut(s) 99, 267
Fsp4HI GCNGC 3 cut(s) 231, 234, 237
GluI GCNGC 3 cut(s) 231, 234, 237
GsuI CTGGAG 1 cut(s) 278
HaeIII GGCC 4 cut(s) 98, 106, 134, 155
Hin1II CATG 2 cut(s) 172, 228
HinfI GANTC 2 cut(s) 181, 206
Hpy166II GTNNAC 2 cut(s) 58, 345
Hpy188I TCNGA 3 cut(s) 180, 205, 265
Hpy188III TCNNGA 2 cut(s) 274, 405
Hpy8I GTNNAC 2 cut(s) 58, 345
Hpy99I CGWCG 1 cut(s) 370
HpyAV CCTTC 2 cut(s) 19, 23
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 6 cut(s) 172, 224, 230, 239, 324, 345
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 236
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 2 cut(s) 172, 228
Kzo9I GATC 1 cut(s) 270
LpnPI CCDG 8 cut(s) 178, 211, 253, 284, 308, 368, 386, 390
Lsp1109I GCAGC 3 cut(s) 220, 223, 242
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 1 cut(s) 211
MalI GATC 1 cut(s) 272
MboI GATC 1 cut(s) 270
MboII GAAGA 2 cut(s) 304, 393
MflI RGATCY 1 cut(s) 270
MhlI GDGCHC 2 cut(s) 9, 347
MlsI TGGCCA 3 cut(s) 98, 106, 134
MluNI TGGCCA 3 cut(s) 98, 106, 134
MmeI TCCRAC 1 cut(s) 140
MnlI CCTC 6 cut(s) 198, 294, 302, 317, 320, 396
Mox20I TGGCCA 3 cut(s) 98, 106, 134
Mph1103I ATGCAT 1 cut(s) 226
MscI TGGCCA 3 cut(s) 98, 106, 134
Msp20I TGGCCA 3 cut(s) 98, 106, 134
MspA1I CMGCKG 1 cut(s) 233
MwoI GCNNNNNNNGC 2 cut(s) 230, 236
NdeII GATC 1 cut(s) 270
NlaIII CATG 2 cut(s) 172, 228
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 11
NmuCI GTSAC 1 cut(s) 211
NsiI ATGCAT 1 cut(s) 226
NspI RCATGY 2 cut(s) 172, 228
PaeI GCATGC 1 cut(s) 172
PfeI GAWTC 2 cut(s) 181, 206
PkrI GCNGC 3 cut(s) 232, 235, 238
Ppu21I YACGTR 1 cut(s) 161
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 195, 407
PstNI CAGNNNCTG 2 cut(s) 215, 236
PsuI RGATCY 1 cut(s) 270
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
SatI GCNGC 3 cut(s) 231, 234, 237
Sau3AI GATC 1 cut(s) 270
Sau96I GGNCC 2 cut(s) 195, 407
SduI GDGCHC 2 cut(s) 9, 347
SetI ASST 7 cut(s) 63, 131, 163, 190, 235, 262, 303
SinI GGWCC 2 cut(s) 195, 407
SnaBI TACGTA 1 cut(s) 161
SphI GCATGC 1 cut(s) 172
StyI CCWWGG 1 cut(s) 129
TaiI ACGT 1 cut(s) 163
TaqI TCGA 2 cut(s) 275, 368
TfiI GAWTC 2 cut(s) 181, 206
TscAI CASTG 2 cut(s) 220, 347
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 3 cut(s) 230, 233, 236
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 1 cut(s) 304
TspRI CASTG 2 cut(s) 220, 347
VneI GTGCAC 1 cut(s) 343
VpaK11BI GGWCC 2 cut(s) 195, 407
XagI CCTNNNNNAGG 1 cut(s) 296
XceI RCATGY 2 cut(s) 172, 228
Zsp2I ATGCAT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.