pycom11g06270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0002875
Physical Location & Seq
Forward (+)
4820604 .. 4821049
446 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g06270.2

Sequence Viewer

Length: 396 bp
ATGCTCCACCTTGGCCAAATTGTACCAAAGTCCATTGGCCTTACGTACCAGCATGCACACTCTGAATCAACCTCGTTGGTCCTGTTCCGATTCAGTTACTGCCAATGCATGTGCAGCTGCTGCAAGCCAAAAAATCACCATTTAGCTTCAGACAGCGATCTCAACACATCCTCGTCTCCTCCAGCAGGTTTTCCTCCTCTTCATTGTCTGCAACCTGAAAACTCGTCAGCGCGCCTATTTCCAGCAACAACCCATCAAGCCAGACAAAATTCCCAGACTTCCTCCTACCTCAGGACCTTCTCATCACCTGTGAATCCCATAGCTCCACCTGCTGCAAAACCCCTGAAACACTTGAGACTAAACATCACCAGGAGAAGTGTTGCTTCGAGTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

14.34

Weight (kDa)

9.58

Isoelectric Point (pI)

70.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 337
Acc36I ACCTGC 2 cut(s) 176, 337
AccII CGCG 1 cut(s) 232
AcoI YGGCCR 1 cut(s) 13
AcsI RAATTY 1 cut(s) 268
AcuI CTGAAG 1 cut(s) 132
AfaI GTAC 3 cut(s) 24, 47, 391
AfiI CCNNNNNNNGG 2 cut(s) 185, 291
AjnI CCWGG 1 cut(s) 368
AluBI AGCT 3 cut(s) 117, 146, 323
AluI AGCT 3 cut(s) 117, 146, 323
Alw26I GTCTC 2 cut(s) 180, 349
AlwNI CAGNNNCTG 2 cut(s) 99, 120
AoxI GGCC 2 cut(s) 13, 37
ApeKI GCWGC 4 cut(s) 114, 117, 120, 332
ApoI RAATTY 1 cut(s) 268
AspLEI GCGC 2 cut(s) 232, 234
AspS9I GGNCC 2 cut(s) 79, 294
AsuHPI GGTGA 3 cut(s) 128, 297, 358
AvaII GGWCC 2 cut(s) 79, 294
AxyI CCTNAGG 1 cut(s) 290
BalI TGGCCA 1 cut(s) 15
BbvI GCAGC 4 cut(s) 104, 107, 126, 319
BccI CCATC 1 cut(s) 261
BciT130I CCWGG 1 cut(s) 370
BcoDI GTCTC 2 cut(s) 180, 349
BfaI CTAG 1 cut(s) 394
BfuAI ACCTGC 2 cut(s) 176, 337
BisI GCNGC 4 cut(s) 115, 118, 121, 333
BlsI GCNGC 4 cut(s) 116, 119, 122, 334
Bme1390I CCNGG 1 cut(s) 370
Bme18I GGWCC 2 cut(s) 79, 294
BmgT120I GGNCC 2 cut(s) 79, 294
BmrFI CCNGG 1 cut(s) 370
BpmI CTGGAG 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 373
BsaAI YACGTR 1 cut(s) 45
BsaJI CCNNGG 1 cut(s) 10
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 291
Bse21I CCTNAGG 1 cut(s) 290
BseBI CCWGG 1 cut(s) 370
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 1 cut(s) 167
BseLI CCNNNNNNNGG 2 cut(s) 185, 291
BseMII CTCAG 1 cut(s) 304
BsePI GCGCGC 1 cut(s) 230
BseRI GAGGAG 2 cut(s) 168, 186
BseXI GCAGC 4 cut(s) 104, 107, 126, 319
BsgI GTGCAG 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 232
BshFI GGCC 2 cut(s) 15, 39
BslI CCNNNNNNNGG 2 cut(s) 185, 291
BsmAI GTCTC 2 cut(s) 180, 349
BsmBI CGTCTC 1 cut(s) 180
BsnI GGCC 2 cut(s) 15, 39
Bsp143I GATC 1 cut(s) 157
BspANI GGCC 2 cut(s) 15, 39
BspCNI CTCAG 1 cut(s) 303
BspFNI CGCG 1 cut(s) 232
BspMI ACCTGC 2 cut(s) 176, 337
BssECI CCNNGG 1 cut(s) 10
BssHII GCGCGC 1 cut(s) 230
BssMI GATC 1 cut(s) 157
BssT1I CCWWGG 1 cut(s) 10
Bst2UI CCWGG 1 cut(s) 370
Bst6I CTCTTC 1 cut(s) 204
BstAPI GCANNNNNTGC 1 cut(s) 120
BstBAI YACGTR 1 cut(s) 45
BstC8I GCNNGC 3 cut(s) 54, 125, 232
BstDEI CTNAG 1 cut(s) 290
BstENI CCTNNNNNAGG 2 cut(s) 183, 289
BstF5I GGATG 1 cut(s) 167
BstFNI CGCG 1 cut(s) 232
BstHHI GCGC 2 cut(s) 232, 234
BstKTI GATC 1 cut(s) 160
BstMAI GTCTC 2 cut(s) 180, 349
BstMBI GATC 1 cut(s) 157
BstMWI GCNNNNNNNGC 3 cut(s) 114, 120, 329
BstNI CCWGG 1 cut(s) 370
BstNSI RCATGY 2 cut(s) 56, 112
BstSCI CCNGG 1 cut(s) 368
BstSNI TACGTA 1 cut(s) 45
BstUI CGCG 1 cut(s) 232
BstV1I GCAGC 4 cut(s) 104, 107, 126, 319
Bsu36I CCTNAGG 1 cut(s) 290
BsuRI GGCC 2 cut(s) 15, 39
BtsCI GGATG 1 cut(s) 167
BveI ACCTGC 2 cut(s) 176, 337
Cac8I GCNNGC 3 cut(s) 54, 125, 232
CaiI CAGNNNCTG 2 cut(s) 99, 120
CfoI GCGC 2 cut(s) 232, 234
Cfr13I GGNCC 2 cut(s) 79, 294
Csp6I GTAC 3 cut(s) 23, 46, 390
CviAII CATG 2 cut(s) 53, 109
CviJI RGCY 7 cut(s) 15, 39, 117, 127, 146, 260, 323
CviKI_1 RGCY 7 cut(s) 15, 39, 117, 127, 146, 260, 323
CviQI GTAC 3 cut(s) 23, 46, 390
DdeI CTNAG 1 cut(s) 290
DpnI GATC 1 cut(s) 159
DpnII GATC 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Eco105I TACGTA 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 10
Eco47I GGWCC 2 cut(s) 79, 294
Eco57I CTGAAG 1 cut(s) 132
Eco81I CCTNAGG 1 cut(s) 290
EcoNI CCTNNNNNAGG 2 cut(s) 183, 289
EcoO109I RGGNCCY 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 368
EcoT14I CCWWGG 1 cut(s) 10
EcoT22I ATGCAT 1 cut(s) 110
ErhI CCWWGG 1 cut(s) 10
Esp3I CGTCTC 1 cut(s) 180
FaeI CATG 2 cut(s) 56, 112
FaiI YATR 3 cut(s) 54, 110, 320
FalI AAGNNNNNCTT 1 cut(s) 367
FatI CATG 2 cut(s) 52, 108
Fnu4HI GCNGC 4 cut(s) 115, 118, 121, 333
FokI GGATG 1 cut(s) 154
Fsp4HI GCNGC 4 cut(s) 115, 118, 121, 333
FspBI CTAG 1 cut(s) 394
GlaI GCGC 2 cut(s) 231, 233
GluI GCNGC 4 cut(s) 115, 118, 121, 333
GsuI CTGGAG 1 cut(s) 165
HaeIII GGCC 2 cut(s) 15, 39
HhaI GCGC 2 cut(s) 232, 234
Hin1II CATG 2 cut(s) 56, 112
Hin6I GCGC 2 cut(s) 230, 232
HinP1I GCGC 2 cut(s) 230, 232
HinfI GANTC 3 cut(s) 65, 90, 313
HphI GGTGA 3 cut(s) 128, 297, 358
Hpy188I TCNGA 3 cut(s) 64, 89, 151
Hpy188III TCNNGA 1 cut(s) 292
HpyAV CCTTC 1 cut(s) 307
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 6 cut(s) 56, 108, 114, 123, 211, 335
HpyF10VI GCNNNNNNNGC 3 cut(s) 114, 120, 329
HpyF3I CTNAG 1 cut(s) 290
HpySE526I ACGT 1 cut(s) 44
Hsp92II CATG 2 cut(s) 56, 112
HspAI GCGC 2 cut(s) 230, 232
Kzo9I GATC 1 cut(s) 157
LmnI GCTCC 2 cut(s) 9, 328
Lsp1109I GCAGC 4 cut(s) 104, 107, 126, 319
MaeI CTAG 1 cut(s) 394
MaeII ACGT 1 cut(s) 44
MaeIII GTNAC 1 cut(s) 95
MalI GATC 1 cut(s) 159
MboI GATC 1 cut(s) 157
MboII GAAGA 1 cut(s) 191
MlsI TGGCCA 1 cut(s) 15
MluCI AATT 2 cut(s) 18, 268
MluNI TGGCCA 1 cut(s) 15
MnlI CCTC 7 cut(s) 82, 181, 189, 204, 207, 292, 299
Mox20I TGGCCA 1 cut(s) 15
Mph1103I ATGCAT 1 cut(s) 110
MscI TGGCCA 1 cut(s) 15
Msp20I TGGCCA 1 cut(s) 15
MspA1I CMGCKG 1 cut(s) 117
MspR9I CCNGG 1 cut(s) 370
MvaI CCWGG 1 cut(s) 370
MvnI CGCG 1 cut(s) 232
MwoI GCNNNNNNNGC 3 cut(s) 114, 120, 329
NdeII GATC 1 cut(s) 157
NlaIII CATG 2 cut(s) 56, 112
NsiI ATGCAT 1 cut(s) 110
NspI RCATGY 2 cut(s) 56, 112
PaeI GCATGC 1 cut(s) 56
PaqCI CACCTGC 1 cut(s) 337
PauI GCGCGC 1 cut(s) 230
PfeI GAWTC 3 cut(s) 65, 90, 313
PkrI GCNGC 4 cut(s) 116, 119, 122, 334
Ppu21I YACGTR 1 cut(s) 45
PpuMI RGGWCCY 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 368
PspGI CCWGG 1 cut(s) 368
PspPI GGNCC 2 cut(s) 79, 294
PspPPI RGGWCCY 1 cut(s) 294
PstNI CAGNNNCTG 2 cut(s) 99, 120
PteI GCGCGC 1 cut(s) 230
PvuII CAGCTG 1 cut(s) 117
RsaI GTAC 3 cut(s) 24, 47, 391
RsaNI GTAC 3 cut(s) 23, 46, 390
SatI GCNGC 4 cut(s) 115, 118, 121, 333
Sau3AI GATC 1 cut(s) 157
Sau96I GGNCC 2 cut(s) 79, 294
ScrFI CCNGG 1 cut(s) 370
SinI GGWCC 2 cut(s) 79, 294
SmlI CTYRAG 1 cut(s) 352
SmoI CTYRAG 1 cut(s) 352
SnaBI TACGTA 1 cut(s) 45
SphI GCATGC 1 cut(s) 56
Sse9I AATT 2 cut(s) 18, 268
SspMI CTAG 1 cut(s) 394
StyD4I CCNGG 1 cut(s) 368
StyI CCWWGG 1 cut(s) 10
TaiI ACGT 1 cut(s) 47
TaqI TCGA 1 cut(s) 386
TasI AATT 2 cut(s) 18, 268
TfiI GAWTC 3 cut(s) 65, 90, 313
TseI GCWGC 4 cut(s) 114, 117, 120, 332
TspDTI ATGAA 1 cut(s) 191
VpaK11BI GGWCC 2 cut(s) 79, 294
XagI CCTNNNNNAGG 2 cut(s) 183, 289
XapI RAATTY 1 cut(s) 268
XceI RCATGY 2 cut(s) 56, 112
XspI CTAG 1 cut(s) 394
Zsp2I ATGCAT 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.