pycom14g16880

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002875
Physical Location & Seq
Forward (+)
19034572 .. 19035006
435 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g16880.2

Sequence Viewer

Length: 360 bp
ATGGCTTTGAAAAAGCCACAAAACAAGGACCTAATTTGGCCACTATCCAACAATCTCCACCTTGGCCAAATTGTACCAAAGTCCATTGGCCTTACGTACCAGCATGCACACTCTGAATCAACCTCGTTGGTCCTGTTCCGATTCAGTCACTGCCAATGCATGTGCAGCTGCTGCAAGCAAAAAAAACATTTAGCTTCAGACAGGATCTCGACACATCCTCGTCTCTTCCAGCAGGTTTTCCTCCTCTTCATTGTCTGCAACTTGGAAACTTGTCAGTGCACCCATTTCCAGCAACATCCGTCGAGCCAAACAAACATTCCTCACACTTCTCATTCTTCAGGACCATCTCATCACCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

120

Amino Acids

13.56

Weight (kDa)

9.05

Isoelectric Point (pI)

33.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 223
AclWI GGATC 1 cut(s) 212
AcoI YGGCCR 2 cut(s) 38, 64
AcuI CTGAAG 2 cut(s) 180, 321
AfaI GTAC 2 cut(s) 75, 98
AgsI TTSAA 1 cut(s) 10
AjuI GAANNNNNNNTTGG 2 cut(s) 300, 332
AluBI AGCT 2 cut(s) 168, 194
AluI AGCT 2 cut(s) 168, 194
Alw21I GWGCWC 1 cut(s) 281
Alw26I GTCTC 1 cut(s) 227
Alw44I GTGCAC 1 cut(s) 277
AlwI GGATC 1 cut(s) 212
AlwNI CAGNNNCTG 2 cut(s) 150, 171
AoxI GGCC 3 cut(s) 38, 64, 88
ApaLI GTGCAC 1 cut(s) 277
ApeKI GCWGC 3 cut(s) 165, 168, 171
AspS9I GGNCC 3 cut(s) 28, 130, 341
AsuHPI GGTGA 1 cut(s) 344
AvaII GGWCC 3 cut(s) 28, 130, 341
BaeGI GKGCMC 1 cut(s) 281
BalI TGGCCA 2 cut(s) 40, 66
Bbv12I GWGCWC 1 cut(s) 281
BbvI GCAGC 3 cut(s) 155, 158, 177
BccI CCATC 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 227
BfuAI ACCTGC 1 cut(s) 223
BisI GCNGC 3 cut(s) 166, 169, 172
BlsI GCNGC 3 cut(s) 167, 170, 173
Bme18I GGWCC 3 cut(s) 28, 130, 341
BmgT120I GGNCC 3 cut(s) 28, 130, 341
BsaAI YACGTR 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 61
BseDI CCNNGG 1 cut(s) 61
BseGI GGATG 2 cut(s) 214, 295
BseRI GAGGAG 1 cut(s) 233
BseSI GKGCMC 1 cut(s) 281
BseXI GCAGC 3 cut(s) 155, 158, 177
BsgI GTGCAG 1 cut(s) 184
BshFI GGCC 3 cut(s) 40, 66, 90
BsiHKAI GWGCWC 1 cut(s) 281
BsmAI GTCTC 1 cut(s) 227
BsmBI CGTCTC 1 cut(s) 227
BsnI GGCC 3 cut(s) 40, 66, 90
Bsp1286I GDGCHC 1 cut(s) 281
Bsp143I GATC 1 cut(s) 204
BspANI GGCC 3 cut(s) 40, 66, 90
BspMI ACCTGC 1 cut(s) 223
BspPI GGATC 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 61
BssMI GATC 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 61
Bst6I CTCTTC 2 cut(s) 230, 251
BstAPI GCANNNNNTGC 1 cut(s) 171
BstBAI YACGTR 1 cut(s) 96
BstC8I GCNNGC 2 cut(s) 105, 176
BstF5I GGATG 2 cut(s) 214, 295
BstKTI GATC 1 cut(s) 207
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 1 cut(s) 204
BstMWI GCNNNNNNNGC 2 cut(s) 165, 171
BstNSI RCATGY 2 cut(s) 107, 163
BstSLI GKGCMC 1 cut(s) 281
BstSNI TACGTA 1 cut(s) 96
BstV1I GCAGC 3 cut(s) 155, 158, 177
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
BsuRI GGCC 3 cut(s) 40, 66, 90
BtsCI GGATG 2 cut(s) 214, 295
BtsI GCAGTG 1 cut(s) 148
BtsIMutI CAGTG 2 cut(s) 148, 281
BveI ACCTGC 1 cut(s) 223
Cac8I GCNNGC 2 cut(s) 105, 176
CaiI CAGNNNCTG 2 cut(s) 150, 171
Cfr13I GGNCC 3 cut(s) 28, 130, 341
Csp6I GTAC 2 cut(s) 74, 97
CviAII CATG 2 cut(s) 104, 160
CviJI RGCY 8 cut(s) 5, 16, 40, 66, 90, 168, 194, 306
CviKI_1 RGCY 8 cut(s) 5, 16, 40, 66, 90, 168, 194, 306
CviQI GTAC 2 cut(s) 74, 97
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
EaeI YGGCCR 2 cut(s) 38, 64
Eam1104I CTCTTC 2 cut(s) 230, 251
EarI CTCTTC 2 cut(s) 230, 251
Eco105I TACGTA 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 61
Eco47I GGWCC 3 cut(s) 28, 130, 341
Eco57I CTGAAG 2 cut(s) 180, 321
EcoO109I RGGNCCY 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 61
EcoT22I ATGCAT 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 61
Esp3I CGTCTC 1 cut(s) 227
FaeI CATG 2 cut(s) 107, 163
FaiI YATR 2 cut(s) 105, 161
FatI CATG 2 cut(s) 103, 159
Fnu4HI GCNGC 3 cut(s) 166, 169, 172
FokI GGATG 2 cut(s) 201, 282
Fsp4HI GCNGC 3 cut(s) 166, 169, 172
GluI GCNGC 3 cut(s) 166, 169, 172
HaeIII GGCC 3 cut(s) 40, 66, 90
Hin1II CATG 2 cut(s) 107, 163
HinfI GANTC 2 cut(s) 116, 141
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 1 cut(s) 279
Hpy188I TCNGA 3 cut(s) 115, 140, 199
Hpy188III TCNNGA 2 cut(s) 208, 339
Hpy8I GTNNAC 1 cut(s) 279
Hpy99I CGWCG 1 cut(s) 304
HpyCH4IV ACGT 1 cut(s) 95
HpyCH4V TGCA 6 cut(s) 107, 159, 165, 174, 258, 279
HpyF10VI GCNNNNNNNGC 2 cut(s) 165, 171
HpySE526I ACGT 1 cut(s) 95
Hsp92II CATG 2 cut(s) 107, 163
Kzo9I GATC 1 cut(s) 204
LpnPI CCDG 7 cut(s) 113, 146, 187, 218, 242, 302, 324
Lsp1109I GCAGC 3 cut(s) 155, 158, 177
MaeII ACGT 1 cut(s) 95
MaeIII GTNAC 1 cut(s) 146
MalI GATC 1 cut(s) 206
MboI GATC 1 cut(s) 204
MboII GAAGA 3 cut(s) 217, 238, 327
MflI RGATCY 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 281
MlsI TGGCCA 2 cut(s) 40, 66
MluCI AATT 2 cut(s) 33, 69
MluNI TGGCCA 2 cut(s) 40, 66
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 5 cut(s) 133, 228, 251, 254, 330
Mox20I TGGCCA 2 cut(s) 40, 66
Mph1103I ATGCAT 1 cut(s) 161
MscI TGGCCA 2 cut(s) 40, 66
Msp20I TGGCCA 2 cut(s) 40, 66
MspA1I CMGCKG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 165, 171
NdeII GATC 1 cut(s) 204
NlaIII CATG 2 cut(s) 107, 163
NmuCI GTSAC 1 cut(s) 146
NsiI ATGCAT 1 cut(s) 161
NspI RCATGY 2 cut(s) 107, 163
PaeI GCATGC 1 cut(s) 107
PfeI GAWTC 2 cut(s) 116, 141
PkrI GCNGC 3 cut(s) 167, 170, 173
Ppu21I YACGTR 1 cut(s) 96
PpuMI RGGWCCY 1 cut(s) 28
Psp5II RGGWCCY 1 cut(s) 28
PspPI GGNCC 3 cut(s) 28, 130, 341
PspPPI RGGWCCY 1 cut(s) 28
PstNI CAGNNNCTG 2 cut(s) 150, 171
PsuI RGATCY 1 cut(s) 204
PvuII CAGCTG 1 cut(s) 168
RsaI GTAC 2 cut(s) 75, 98
RsaNI GTAC 2 cut(s) 74, 97
SatI GCNGC 3 cut(s) 166, 169, 172
Sau3AI GATC 1 cut(s) 204
Sau96I GGNCC 3 cut(s) 28, 130, 341
SduI GDGCHC 1 cut(s) 281
SetI ASST 8 cut(s) 33, 63, 98, 125, 170, 196, 237, 357
SinI GGWCC 3 cut(s) 28, 130, 341
SnaBI TACGTA 1 cut(s) 96
SphI GCATGC 1 cut(s) 107
Sse9I AATT 2 cut(s) 33, 69
StyI CCWWGG 1 cut(s) 61
TaiI ACGT 1 cut(s) 98
TaqI TCGA 2 cut(s) 209, 302
TasI AATT 2 cut(s) 33, 69
TfiI GAWTC 2 cut(s) 116, 141
TscAI CASTG 2 cut(s) 155, 281
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 3 cut(s) 165, 168, 171
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 1 cut(s) 238
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 2 cut(s) 155, 281
VneI GTGCAC 1 cut(s) 277
VpaK11BI GGWCC 3 cut(s) 28, 130, 341
XceI RCATGY 2 cut(s) 107, 163
Zsp2I ATGCAT 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.