pycom04g14780
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0003416
Physical Location & Seq
Reverse (-)
17576109 .. 17578645
2537 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2415 bp
ATGTTCATGGAAAATAAGTTGTTGTTTGTCATCCATGCCTTACTCTTTGTTCAGCTATTAATTGTCACTTACCAGCCTGTTTCGGGCCAGCGAGGGAATGATGGAGTGATTGTGACTCAGTCCGATTACCAAGCTCTTCGAGCATTCAAGCGTGAGCTTGTTGATTTCACTGGTGTACTGCGCAGCTGGAATGACAGCGGACATGGAGCCTGCTTGGGCGGGTGGGCAGGGATCAAGTGTGTGAACGGACAGGTCATTGCAATCCAGCTTCCGTGGAAGAGGCTCGGGGGCAGAATCTCGGAAAAGATTGGGCAGCTGCAAGCGCTTCGGAAGCTCAGTCTGCACGACAATGTCTTGGCTGGCTCTGTCCCTTTGTCTCTTGGCTTCCTTCCCAATCTCAGAGGGGTTTACCTCTTCAACAACCGGCTTTCGGGTTCTGTCCCGCCTTCGATTGGTAACTGCCCTCTTCTTCAAACTCTTGATTTGAGCAATAATTCGCTTACAGGTACAATCCCTTCTAGTCTTGCAAATTCTACCAAGCTGTACAGACTTAATCTGAGCTTCAATTCGCTTTCGGGTTTCATCCCAACTAGTCTCACCAAGTCCCCTTCCCTCATCATCCTTGCCCTCCAACACAACAACCTCTCTGGTCCTGTTCCAAGCACTTGGGGTGCTGGAAATCGAAATCATAGTTACAAACTTACGATCCTGACCCTTGATAATAATCTGATTTCCGGAACCATTCCAAGTTCTCTGAGCAAATTGGGTTTTCTTGAAGAGATTTATTTGAACAACAACCAGATTTCCGGGACCATTCCCGACGAAATAGGGGAGCTCACAAGGCTCCAGAAGCTAGACTTATCCAACAATGCCATCAATGGCAGCTTCCCTTCTAGCTTCTCCAACCTCTCCTCCCTTGTTTCGTTGAACCTTGAGGGCAACCGCCTCAATAACCAAATCCCGGAAGGCCTGGAAAGGTTGCAGAACCTCTCTGTACTTAACTTGAAGAAAAACAATTTCAGTGGCCACATTCCAGCATCTGTTGGCAATATCTCTGGAATTTACCAACTCGATTTATCCGAAAACAACTTCAGTGGTAAAATTCCTCCTTCACTTTCCAGCCTAGCCAATCTCACTTCCTTCAATGTTTCTCACAACAATCTCTCTGGCCCCGTCCCTTCTCTCCTCTCGAAAAAGTTCAATTCTAGCTCTTTTGCGGGCAATCTTCAGCTGTGCGGGTACAGCGCTTCAACTCCGTGTTCTTCTCCCCCGCCTCAGATTCTTCCATCTTCTCCGCCAACAGAGCAGCCTTTGAAGAAAAAACATCACCACAAACTTAGTACGAAGGACAAAATTCTCATAGCGGCGGGTTCCCTCCTGGCGGTTCTGCTTCTACTCTGCTGCATTCTGCTTGTTTGTTTGGTCAGGAAAAGGTCTGCTTCAAAAGGAATGAATGGTACAACGGCTAAGCAAGCCGCTGATGCTGGGATCGCTGCCAAGGCAGTTCCTGGTGGTACCGGAGTTGAATATGGCGGTGAAGCGGGTGGGAAGCTTGTTCACTTTGATGGGCCGTTTGTTTTCACAGCTGACGACCTGCTGTGTGCCACTGCTGAAATAATGGGGAAGAGCACATATGGGACTGCATACAAGGCAACATTAGAGGAAGGAAATGAAGTTGCGGTGAAGAGATTGAGGGAAAAGACAACGAAAGGTCAGAAGGAATTCGAAACTGAAGCTGCAGCAATTGGGAAGATTCGCCACCCGAATCTCTTGGCTCTGAGGGCTTATTACTTGGGACCTAAGGGAGAGAAGCTTCTCGTCTTCGATTACATGTCTAAGGGTAGCCTTGCATCTTTCCTTCATGCTCGAGGGCCAGACACCATCATTGATTGGCCAACAAGGATGAACATAGCGATCGGCATCACTCGTGGACTATGCTACCTCCACAACGACGAGAACATTGTACATGGCAATCTCACATCCAGCAACATTCTACTTGATGAGCAGACCAATGCTCACATTGCAGACTTTGGCCTTTCGCGCCTCATGACTGCGGCCGCCAACACCACTGTGATCGCCACCGCAGGAACTCTCGGCTACAACGCACCTGAGCTCTCAAAGTCCAAGAAGGCCACAACAAAGACTGATGTGTACAGCCTTGGGGTGCTCATACTGGAGCTCCTGACAGGAAAATCCCCCGGGGAACCGATGAACAGCATGGATTTGCCCCAGTGGGTGGCATCAATTGTGAAAGAGGAGTGGACAAATGAAGTTTTTGACTTGGAGCTCGTGAGGGATGTGCAAACCATAGGTGACCTGCTGCTTAACACATTGAAGTTGGCTCCATTGTGTGGATCCATCGCCGGCTGCGCGGCCGGAAGCTCAGCAGGTTCTGCAGCAGCTGGAGGAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

805

Amino Acids

85.78

Weight (kDa)

8.85

Isoelectric Point (pI)

32.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 40 - 80 1.1e-07 Leucine rich repeat N-terminal domain
LRR_4 PF12799 131 - 169 4.5e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 156 - 215 6.7e-07 Leucine rich repeat
LRR_8 PF13855 233 - 292 8e-09 Leucine rich repeat
LRR_14 PF23598 252 - 388 1.9e-11 Leucine-rich repeat region
LRR_8 PF13855 281 - 340 9.2e-08 Leucine rich repeat
Pkinase PF00069 537 - 736 1.7e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 538 - 746 1.1e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 182
Acc36I ACCTGC 3 cut(s) 1602, 2325, 2378
Acc65I GGTACC 1 cut(s) 1514
AccB1I GGYRCC 1 cut(s) 1514
AccB7I CCANNNNNTGG 2 cut(s) 2236, 2351
AccII CGCG 2 cut(s) 2041, 2372
AccIII TCCGGA 1 cut(s) 734
AclWI GGATC 5 cut(s) 239, 700, 1496, 2349, 2362
AcoI YGGCCR 4 cut(s) 1024, 1892, 2055, 2373
AcsI RAATTY 5 cut(s) 529, 1059, 1101, 1353, 1721
AcuI CTGAAG 3 cut(s) 1075, 1211, 1752
AfeI AGCGCT 2 cut(s) 324, 1246
AfiI CCNNNNNNNGG 7 cut(s) 83, 430, 452, 961, 1381, 2236, 2351
AflIII ACRYGT 1 cut(s) 1830
AhlI ACTAGT 1 cut(s) 590
AjnI CCWGG 3 cut(s) 969, 1377, 1507
AleI CACNNNNGTG 1 cut(s) 2069
Alw21I GWGCWC 6 cut(s) 837, 1631, 2115, 2169, 2181, 2289
Alw26I GTCTC 2 cut(s) 381, 599
AlwI GGATC 5 cut(s) 239, 700, 1496, 2349, 2362
AlwNI CAGNNNCTG 4 cut(s) 1040, 1508, 2393, 2402
Ama87I CYCGRG 3 cut(s) 284, 1866, 2198
Aor13HI TCCGGA 1 cut(s) 734
Aor51HI AGCGCT 2 cut(s) 324, 1246
ApoI RAATTY 5 cut(s) 529, 1059, 1101, 1353, 1721
AseI ATTAAT 1 cut(s) 59
Asp700I GAANNNNTTC 2 cut(s) 1196, 1721
Asp718I GGTACC 1 cut(s) 1514
AspLEI GCGC 5 cut(s) 183, 325, 1247, 2043, 2372
AspS9I GGNCC 7 cut(s) 85, 650, 810, 1169, 1568, 1796, 1871
AsuC2I CCSGG 4 cut(s) 808, 962, 2199, 2200
AsuHPI GGTGA 5 cut(s) 589, 1319, 1547, 1693, 2324
AsuII TTCGAA 1 cut(s) 1725
AvaI CYCGRG 3 cut(s) 284, 1866, 2198
AvaII GGWCC 3 cut(s) 650, 810, 1796
AxyI CCTNAGG 1 cut(s) 1800
BaeI ACNNNNGTAYC 2 cut(s) 1506, 1539
BalI TGGCCA 2 cut(s) 1026, 1894
BamHI GGATCC 1 cut(s) 2354
BanI GGYRCC 1 cut(s) 1514
BanII GRGCYC 4 cut(s) 837, 2115, 2181, 2289
BarI GAAGNNNNNNTAC 2 cut(s) 499, 531
BauI CACGAG 2 cut(s) 1926, 2288
BbsI GAAGAC 1 cut(s) 1813
Bbv12I GWGCWC 6 cut(s) 837, 1631, 2115, 2169, 2181, 2289
BccI CCATC 6 cut(s) 95, 881, 1294, 1559, 1889, 2366
BceAI ACGGC 2 cut(s) 1479, 1555
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 3 cut(s) 971, 1379, 1509
BcnI CCSGG 4 cut(s) 808, 962, 2199, 2200
BcoDI GTCTC 2 cut(s) 381, 599
BcuI ACTAGT 1 cut(s) 590
BfaI CTAG 6 cut(s) 519, 591, 854, 894, 1124, 1206
BfmI CTRYAG 2 cut(s) 1737, 2394
BfoI RGCGCY 2 cut(s) 326, 1248
BfuAI ACCTGC 3 cut(s) 1602, 2325, 2378
BlpI GCTNAGC 2 cut(s) 1467, 2383
Bme1390I CCNGG 7 cut(s) 808, 962, 971, 1379, 1509, 2199, 2200
Bme18I GGWCC 3 cut(s) 650, 810, 1796
BmeT110I CYCGRG 3 cut(s) 284, 1866, 2198
BmgT120I GGNCC 7 cut(s) 85, 650, 810, 1169, 1568, 1796, 1871
BmrFI CCNGG 7 cut(s) 808, 962, 971, 1379, 1509, 2199, 2200
BmrI ACTGGG 1 cut(s) 2224
BmsI GCATC 5 cut(s) 1046, 1471, 1859, 1929, 2249
BmuI ACTGGG 1 cut(s) 2224
BpiI GAAGAC 1 cut(s) 1813
BpmI CTGGAG 2 cut(s) 830, 2195
Bpu10I CCTNAGC 1 cut(s) 2109
Bpu1102I GCTNAGC 2 cut(s) 1467, 2383
Bpu14I TTCGAA 1 cut(s) 1725
BpuEI CTTGAG 1 cut(s) 953
BpuMI CCSGG 4 cut(s) 808, 962, 2199, 2200
BsaBI GATNNNNATC 2 cut(s) 723, 1919
BsaJI CCNNGG 6 cut(s) 272, 1497, 2158, 2197, 2198, 2199
BsaWI WCCGGW 2 cut(s) 734, 1517
BsaXI ACNNNNNCTCC 6 cut(s) 96, 126, 896, 926, 2163, 2193
Bsc4I CCNNNNNNNGG 7 cut(s) 83, 430, 452, 961, 1381, 2236, 2351
Bse118I RCCGGY 2 cut(s) 423, 2363
Bse1I ACTGG 3 cut(s) 175, 2178, 2230
Bse21I CCTNAGG 1 cut(s) 1800
Bse3DI GCAATG 2 cut(s) 255, 2019
Bse8I GATNNNNATC 2 cut(s) 723, 1919
BseAI TCCGGA 1 cut(s) 734
BseBI CCWGG 3 cut(s) 971, 1379, 1509
BseDI CCNNGG 6 cut(s) 272, 1497, 2158, 2197, 2198, 2199
BseGI GGATG 6 cut(s) 30, 582, 618, 1908, 1979, 2302
BseJI GATNNNNATC 2 cut(s) 723, 1919
BseLI CCNNNNNNNGG 7 cut(s) 83, 430, 452, 961, 1381, 2236, 2351
BseMI GCAATG 2 cut(s) 255, 2019
BseMII CTCAG 9 cut(s) 131, 349, 412, 548, 746, 1289, 1769, 2100, 2397
BseNI ACTGG 3 cut(s) 175, 2178, 2230
BseRI GAGGAG 3 cut(s) 901, 1175, 2270
BseX3I CGGCCG 2 cut(s) 2055, 2373
BseYI CCCAGC 1 cut(s) 1484
BsgI GTGCAG 1 cut(s) 326
Bsh1236I CGCG 2 cut(s) 2041, 2372
Bsh1285I CGRYCG 3 cut(s) 1917, 2058, 2376
BshNI GGYRCC 1 cut(s) 1514
BsiEI CGRYCG 3 cut(s) 1917, 2058, 2376
BsiHKAI GWGCWC 6 cut(s) 837, 1631, 2115, 2169, 2181, 2289
BsiHKCI CYCGRG 3 cut(s) 284, 1866, 2198
BsiSI CCGG 8 cut(s) 424, 735, 807, 962, 1518, 2199, 2364, 2376
BslFI GGGAC 7 cut(s) 353, 425, 589, 823, 1160, 1651, 1809
BslI CCNNNNNNNGG 7 cut(s) 83, 430, 452, 961, 1381, 2236, 2351
BsmAI GTCTC 2 cut(s) 381, 599
BsmFI GGGAC 7 cut(s) 353, 425, 589, 823, 1160, 1651, 1809
BsmI GAATGC 2 cut(s) 143, 1404
BsoBI CYCGRG 3 cut(s) 284, 1866, 2198
Bsp119I TTCGAA 1 cut(s) 1725
Bsp1286I GDGCHC 6 cut(s) 837, 1631, 2115, 2169, 2181, 2289
Bsp13I TCCGGA 1 cut(s) 734
Bsp1407I TGTACA 3 cut(s) 543, 1963, 2151
Bsp143I GATC 6 cut(s) 231, 705, 1488, 1914, 2073, 2354
Bsp1720I GCTNAGC 2 cut(s) 1467, 2383
BspCNI CTCAG 9 cut(s) 130, 348, 411, 549, 747, 1288, 1770, 2101, 2396
BspEI TCCGGA 1 cut(s) 734
BspFNI CGCG 2 cut(s) 2041, 2372
BspHI TCATGA 1 cut(s) 2046
BspMAI CTGCAG 2 cut(s) 1741, 2398
BspMI ACCTGC 3 cut(s) 1602, 2325, 2378
BspPI GGATC 5 cut(s) 239, 700, 1496, 2349, 2362
BspQI GCTCTTC 2 cut(s) 141, 1619
BspT104I TTCGAA 1 cut(s) 1725
BspT107I GGYRCC 1 cut(s) 1514
BsrDI GCAATG 2 cut(s) 255, 2019
BsrFI RCCGGY 2 cut(s) 423, 2363
BsrGI TGTACA 3 cut(s) 543, 1963, 2151
BsrI ACTGG 3 cut(s) 175, 2178, 2230
BssAI RCCGGY 2 cut(s) 423, 2363
BssECI CCNNGG 6 cut(s) 272, 1497, 2158, 2197, 2198, 2199
BssMI GATC 6 cut(s) 231, 705, 1488, 1914, 2073, 2354
BssSI CACGAG 2 cut(s) 1926, 2288
BssT1I CCWWGG 2 cut(s) 1497, 2158
Bst2BI CACGAG 2 cut(s) 1926, 2288
Bst2UI CCWGG 3 cut(s) 971, 1379, 1509
Bst4CI ACNGT 1 cut(s) 2071
Bst6I CTCTTC 7 cut(s) 141, 272, 419, 471, 771, 1619, 1679
BstAPI GCANNNNNTGC 1 cut(s) 2393
BstAUI TGTACA 3 cut(s) 543, 1963, 2151
BstBI TTCGAA 1 cut(s) 1725
BstC8I GCNNGC 7 cut(s) 89, 211, 321, 361, 1219, 1473, 2365
BstDSI CCRYGG 1 cut(s) 272
BstEII GGTNACC 1 cut(s) 2312
BstF5I GGATG 6 cut(s) 30, 582, 618, 1908, 1979, 2302
BstFNI CGCG 2 cut(s) 2041, 2372
BstH2I RGCGCY 2 cut(s) 326, 1248
BstHHI GCGC 5 cut(s) 183, 325, 1247, 2043, 2372
BstKTI GATC 6 cut(s) 234, 708, 1491, 1917, 2076, 2357
BstMAI GTCTC 2 cut(s) 381, 599
BstMBI GATC 6 cut(s) 231, 705, 1488, 1914, 2073, 2354
BstMCI CGRYCG 3 cut(s) 1917, 2058, 2376
BstNI CCWGG 3 cut(s) 971, 1379, 1509
BstNSI RCATGY 1 cut(s) 1834
BstPI GGTNACC 1 cut(s) 2312
BstSCI CCNGG 7 cut(s) 806, 960, 969, 1377, 1507, 2197, 2198
BstSFI CTRYAG 2 cut(s) 1737, 2394
BstUI CGCG 2 cut(s) 2041, 2372
BstV2I GAAGAC 1 cut(s) 1813
BstX2I RGATCY 1 cut(s) 2354
BstXI CCANNNNNNTGG 1 cut(s) 666
BstYI RGATCY 1 cut(s) 2354
BstZI CGGCCG 2 cut(s) 2055, 2373
Bsu36I CCTNAGG 1 cut(s) 1800
BtgI CCRYGG 1 cut(s) 272
BtgZI GCGATG 1 cut(s) 2344
BtsCI GGATG 6 cut(s) 30, 582, 618, 1908, 1979, 2302
BtsI GCAGTG 1 cut(s) 1605
BtsIMutI CAGTG 6 cut(s) 168, 1027, 1099, 1605, 2067, 2237
BveI ACCTGC 3 cut(s) 1602, 2325, 2378
Cac8I GCNNGC 7 cut(s) 89, 211, 321, 361, 1219, 1473, 2365
CaiI CAGNNNCTG 4 cut(s) 1040, 1508, 2393, 2402
CciI TCATGA 1 cut(s) 2046
CciNI GCGGCCGC 1 cut(s) 2055
CfoI GCGC 5 cut(s) 183, 325, 1247, 2043, 2372
Cfr10I RCCGGY 2 cut(s) 423, 2363
Cfr13I GGNCC 7 cut(s) 85, 650, 810, 1169, 1568, 1796, 1871
Cfr9I CCCGGG 1 cut(s) 2198
CspCI CAANNNNNGTGG 4 cut(s) 1003, 1038, 1075, 1110
CviAII CATG 8 cut(s) 7, 35, 203, 1831, 1862, 1967, 2047, 2218
DpnI GATC 6 cut(s) 233, 707, 1490, 1916, 2075, 2356
DpnII GATC 6 cut(s) 231, 705, 1488, 1914, 2073, 2354
EaeI YGGCCR 4 cut(s) 1024, 1892, 2055, 2373
EagI CGGCCG 2 cut(s) 2055, 2373
Eam1104I CTCTTC 7 cut(s) 141, 272, 419, 471, 771, 1619, 1679
EarI CTCTTC 7 cut(s) 141, 272, 419, 471, 771, 1619, 1679
EciI GGCGGA 1 cut(s) 1284
Ecl136II GAGCTC 4 cut(s) 835, 2113, 2179, 2287
EclXI CGGCCG 2 cut(s) 2055, 2373
Eco130I CCWWGG 2 cut(s) 1497, 2158
Eco147I AGGCCT 1 cut(s) 969
Eco24I GRGCYC 4 cut(s) 837, 2115, 2181, 2289
Eco47I GGWCC 3 cut(s) 650, 810, 1796
Eco47III AGCGCT 2 cut(s) 324, 1246
Eco52I CGGCCG 2 cut(s) 2055, 2373
Eco53kI GAGCTC 4 cut(s) 835, 2113, 2179, 2287
Eco57I CTGAAG 3 cut(s) 1075, 1211, 1752
Eco81I CCTNAGG 1 cut(s) 1800
Eco88I CYCGRG 3 cut(s) 284, 1866, 2198
Eco91I GGTNACC 1 cut(s) 2312
EcoICRI GAGCTC 4 cut(s) 835, 2113, 2179, 2287
EcoO109I RGGNCCY 1 cut(s) 1796
EcoO65I GGTNACC 1 cut(s) 2312
EcoRI GAATTC 1 cut(s) 1721
EcoRII CCWGG 3 cut(s) 969, 1377, 1507
EcoT14I CCWWGG 2 cut(s) 1497, 2158
EcoT38I GRGCYC 4 cut(s) 837, 2115, 2181, 2289
ErhI CCWWGG 2 cut(s) 1497, 2158
FaeI CATG 8 cut(s) 10, 38, 206, 1834, 1865, 1970, 2050, 2221
FalI AAGNNNNNCTT 4 cut(s) 842, 874, 1423, 1455
FaqI GGGAC 7 cut(s) 353, 425, 589, 823, 1160, 1651, 1809
FatI CATG 8 cut(s) 6, 34, 202, 1830, 1861, 1966, 2046, 2217
FauI CCCGC 7 cut(s) 212, 450, 1210, 1229, 1278, 1360, 1534
FauNDI CATATG 1 cut(s) 1633
FokI GGATG 6 cut(s) 17, 569, 605, 1915, 1966, 2309
FriOI GRGCYC 4 cut(s) 837, 2115, 2181, 2289
FspBI CTAG 6 cut(s) 519, 591, 854, 894, 1124, 1206
FspI TGCGCA 1 cut(s) 182
GlaI GCGC 5 cut(s) 182, 324, 1246, 2042, 2371
GsaI CCCAGC 1 cut(s) 1488
GsuI CTGGAG 2 cut(s) 830, 2195
HaeII RGCGCY 2 cut(s) 326, 1248
HapII CCGG 8 cut(s) 424, 735, 807, 962, 1518, 2199, 2364, 2376
HhaI GCGC 5 cut(s) 183, 325, 1247, 2043, 2372
Hin1II CATG 8 cut(s) 10, 38, 206, 1834, 1865, 1970, 2050, 2221
Hin6I GCGC 5 cut(s) 181, 323, 1245, 2041, 2370
HinP1I GCGC 5 cut(s) 181, 323, 1245, 2041, 2370
HindIII AAGCTT 2 cut(s) 1550, 1811
HinfI GANTC 5 cut(s) 115, 294, 1279, 1753, 1765
HpaII CCGG 8 cut(s) 424, 735, 807, 962, 1518, 2199, 2364, 2376
HphI GGTGA 5 cut(s) 589, 1319, 1547, 1693, 2324
Hpy166II GTNNAC 7 cut(s) 176, 244, 409, 1558, 1931, 2152, 2262
Hpy8I GTNNAC 7 cut(s) 176, 244, 409, 1558, 1931, 2152, 2262
Hpy99I CGWCG 2 cut(s) 824, 1955
HpyCH4III ACNGT 1 cut(s) 2071
Hsp92II CATG 8 cut(s) 10, 38, 206, 1834, 1865, 1970, 2050, 2221
HspAI GCGC 5 cut(s) 181, 323, 1245, 2041, 2370
Kpn2I TCCGGA 1 cut(s) 734
KpnI GGTACC 1 cut(s) 1518
KroI GCCGGC 1 cut(s) 2363
KroNI GCCGGC 1 cut(s) 2365
Kzo9I GATC 6 cut(s) 231, 705, 1488, 1914, 2073, 2354
LguI GCTCTTC 2 cut(s) 141, 1619
LmnI GCTCC 7 cut(s) 206, 832, 849, 2176, 2184, 2284, 2347
LweI GCATC 5 cut(s) 1046, 1471, 1859, 1929, 2249
MaeI CTAG 6 cut(s) 519, 591, 854, 894, 1124, 1206
MaeIII GTNAC 5 cut(s) 64, 112, 455, 692, 2312
MalI GATC 6 cut(s) 233, 707, 1490, 1916, 2075, 2356
MboI GATC 6 cut(s) 231, 705, 1488, 1914, 2073, 2354
MfeI CAATTG 2 cut(s) 1743, 2244
MflI RGATCY 1 cut(s) 2354
MhlI GDGCHC 6 cut(s) 837, 1631, 2115, 2169, 2181, 2289
MlsI TGGCCA 2 cut(s) 1026, 1894
MluNI TGGCCA 2 cut(s) 1026, 1894
MlyI GAGTC 1 cut(s) 109
MmeI TCCRAC 3 cut(s) 655, 888, 927
Mox20I TGGCCA 2 cut(s) 1026, 1894
MroI TCCGGA 1 cut(s) 734
MroNI GCCGGC 1 cut(s) 2363
MroXI GAANNNNTTC 2 cut(s) 1196, 1721
MscI TGGCCA 2 cut(s) 1026, 1894
MseI TTAA 5 cut(s) 59, 552, 999, 2325, 2413
MslI CAYNNNNRTG 3 cut(s) 348, 1563, 2069
Msp20I TGGCCA 2 cut(s) 1026, 1894
MspA1I CMGCKG 7 cut(s) 186, 198, 316, 1231, 1478, 1586, 2402
MspI CCGG 8 cut(s) 424, 735, 807, 962, 1518, 2199, 2364, 2376
MspR9I CCNGG 7 cut(s) 808, 962, 971, 1379, 1509, 2199, 2200
MunI CAATTG 2 cut(s) 1743, 2244
Mva1269I GAATGC 2 cut(s) 143, 1404
MvaI CCWGG 3 cut(s) 971, 1379, 1509
MvnI CGCG 2 cut(s) 2041, 2372
NaeI GCCGGC 1 cut(s) 2365
NciI CCSGG 4 cut(s) 808, 962, 2199, 2200
NdeI CATATG 1 cut(s) 1633
NdeII GATC 6 cut(s) 231, 705, 1488, 1914, 2073, 2354
NgoMIV GCCGGC 1 cut(s) 2363
NlaIII CATG 8 cut(s) 10, 38, 206, 1834, 1865, 1970, 2050, 2221
NmeAIII GCCGAG 1 cut(s) 2073
NmuCI GTSAC 3 cut(s) 64, 112, 2312
NotI GCGGCCGC 1 cut(s) 2055
NsbI TGCGCA 1 cut(s) 182
NspI RCATGY 1 cut(s) 1834
NspV TTCGAA 1 cut(s) 1725
OliI CACNNNNGTG 1 cut(s) 2069
PaeR7I CTCGAG 1 cut(s) 1866
PagI TCATGA 1 cut(s) 2046
PceI AGGCCT 1 cut(s) 969
PciI ACATGT 1 cut(s) 1830
PciSI GCTCTTC 2 cut(s) 141, 1619
PcsI WCGNNNNNNNCGW 1 cut(s) 1077
PctI GAATGC 2 cut(s) 143, 1404
PdiI GCCGGC 1 cut(s) 2365
PdmI GAANNNNTTC 2 cut(s) 1196, 1721
PfeI GAWTC 4 cut(s) 294, 1279, 1753, 1765
PflFI GACNNNGTC 2 cut(s) 118, 350
PflMI CCANNNNNTGG 2 cut(s) 2236, 2351
PfoI TCCNGGA 2 cut(s) 806, 960
Ple19I CGATCG 1 cut(s) 1917
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PpuMI RGGWCCY 1 cut(s) 1796
PscI ACATGT 1 cut(s) 1830
PshBI ATTAAT 1 cut(s) 59
Psp124BI GAGCTC 4 cut(s) 837, 2115, 2181, 2289
Psp5II RGGWCCY 1 cut(s) 1796
Psp6I CCWGG 3 cut(s) 969, 1377, 1507
PspEI GGTNACC 1 cut(s) 2312
PspFI CCCAGC 1 cut(s) 1484
PspGI CCWGG 3 cut(s) 969, 1377, 1507
PspPI GGNCC 7 cut(s) 85, 650, 810, 1169, 1568, 1796, 1871
PspPPI RGGWCCY 1 cut(s) 1796
PspXI VCTCGAGB 1 cut(s) 1866
PstI CTGCAG 2 cut(s) 1741, 2398
PstNI CAGNNNCTG 4 cut(s) 1040, 1508, 2393, 2402
PsuI RGATCY 1 cut(s) 2354
PsyI GACNNNGTC 2 cut(s) 118, 350
PvuI CGATCG 1 cut(s) 1917
PvuII CAGCTG 5 cut(s) 186, 316, 1231, 1586, 2402
RseI CAYNNNNRTG 3 cut(s) 348, 1563, 2069
SacI GAGCTC 4 cut(s) 837, 2115, 2181, 2289
SapI GCTCTTC 2 cut(s) 141, 1619
SaqAI TTAA 5 cut(s) 59, 552, 999, 2325, 2413
Sau3AI GATC 6 cut(s) 231, 705, 1488, 1914, 2073, 2354
Sau96I GGNCC 7 cut(s) 85, 650, 810, 1169, 1568, 1796, 1871
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 7 cut(s) 808, 962, 971, 1379, 1509, 2199, 2200
SduI GDGCHC 6 cut(s) 837, 1631, 2115, 2169, 2181, 2289
SfaNI GCATC 5 cut(s) 1046, 1471, 1859, 1929, 2249
SfcI CTRYAG 2 cut(s) 1737, 2394
Sfr274I CTCGAG 1 cut(s) 1866
SfuI TTCGAA 1 cut(s) 1725
SinI GGWCC 3 cut(s) 650, 810, 1796
SlaI CTCGAG 1 cut(s) 1866
SmaI CCCGGG 1 cut(s) 2200
SmiMI CAYNNNNRTG 3 cut(s) 348, 1563, 2069
SmlI CTYRAG 2 cut(s) 932, 1866
SmoI CTYRAG 2 cut(s) 932, 1866
SpeI ACTAGT 1 cut(s) 590
SseBI AGGCCT 1 cut(s) 969
SspMI CTAG 6 cut(s) 519, 591, 854, 894, 1124, 1206
SstI GAGCTC 4 cut(s) 837, 2115, 2181, 2289
StuI AGGCCT 1 cut(s) 969
StyD4I CCNGG 7 cut(s) 806, 960, 969, 1377, 1507, 2197, 2198
StyI CCWWGG 2 cut(s) 1497, 2158
TaaI ACNGT 1 cut(s) 2071
TaqI TCGA 8 cut(s) 139, 449, 682, 1071, 1190, 1725, 1824, 1867
TatI WGTACW 5 cut(s) 175, 543, 994, 1963, 2151
TauI GCSGC 5 cut(s) 1367, 1478, 2057, 2060, 2375
TfiI GAWTC 4 cut(s) 294, 1279, 1753, 1765
Tru1I TTAA 5 cut(s) 59, 552, 999, 2325, 2413
Tru9I TTAA 5 cut(s) 59, 552, 999, 2325, 2413
TscAI CASTG 6 cut(s) 175, 1027, 1099, 1612, 2074, 2237
TseFI GTSAC 3 cut(s) 64, 112, 2312
Tsp45I GTSAC 3 cut(s) 64, 112, 2312
TspDTI ATGAA 7 cut(s) 571, 1466, 1686, 1850, 1919, 2225, 2283
TspGWI ACGGA 3 cut(s) 261, 261, 1245
TspMI CCCGGG 1 cut(s) 2198
TspRI CASTG 6 cut(s) 175, 1027, 1099, 1612, 2074, 2237
Tth111I GACNNNGTC 2 cut(s) 118, 350
Van91I CCANNNNNTGG 2 cut(s) 2236, 2351
VpaK11BI GGWCC 3 cut(s) 650, 810, 1796
VspI ATTAAT 1 cut(s) 59
XapI RAATTY 5 cut(s) 529, 1059, 1101, 1353, 1721
XceI RCATGY 1 cut(s) 1834
XhoI CTCGAG 1 cut(s) 1866
XmaI CCCGGG 1 cut(s) 2198
XmnI GAANNNNTTC 2 cut(s) 1196, 1721
XspI CTAG 6 cut(s) 519, 591, 854, 894, 1124, 1206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.