pycom04g20010

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005029
Physical Location & Seq
Reverse (-)
21584961 .. 21586913
1953 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 756 bp
ATGTGCTGGCTGTTAAGAGTACAAATCTTGATGATGAGTCCAGAGTCTCCAAATACAGAGCCCCCCAAGAGTTCACAGCCAACTTCACCTCAGTCATCAGGAGAGGGTGCGGAACATGATACTAAGAAGCATGCTAATGAAGAAGATATTTGGTCTCTGACTTCCTCAACTGCTGCATCTAGAACACCTAAATTCAAGGTTACAGTTGGACAAGCTCCCACGTTTAGGATCTACGAACGTATAGAGAAAAGGAAAGAGTTCTACTCGAAGTTGGAGGAAAAGCATCAAGCTATGGAGGCAGAGAGAAGCCAGTGGGAAGCAAGGGCCAAGGAAGAGCAAGAAGCAGCCATCAAGGCACTGAGGAAGAGCATGGTCTTCAAAGCAAATCCAATACCTAGTTTCATTTACGAGCCCCCTCCTCCTAAGGCTGAACGCAAGAAGCTTCCACTCACTCGGCCCAAGTCACCAAAACTAAACAGTCTAAGCCGGAGGAAGAGCTATGGCGATGCAATCACCAACTCATCCGCAGAGGAAAAAAAGAAAGGCTGTTCTCGGGCACAACGTCACAGCCTTGGCACTCAGAAGGAAGAATCCACGCCTCCCAAAAGTAAGGCTCAGTCCGGAAGGCGAAACAGCCTTGGCGGTTGCAAGACCAAAGACCATTCGAAACACGGAAAGGAAACAACGGAAACATCTTCAAAGACCGCCGAGCACACATCGGATTCGAATGACGACGGACACAAAAACTTCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

252

Amino Acids

28.19

Weight (kDa)

9.63

Isoelectric Point (pI)

61.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 74 - 146 1.1e-23 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 620
AciI CCGC 4 cut(s) 110, 525, 642, 705
AclWI GGATC 1 cut(s) 236
AcsI RAATTY 1 cut(s) 191
AfaI GTAC 1 cut(s) 21
AfiI CCNNNNNNNGG 1 cut(s) 225
AgsI TTSAA 3 cut(s) 196, 379, 699
AluBI AGCT 4 cut(s) 215, 290, 442, 498
AluI AGCT 4 cut(s) 215, 290, 442, 498
Alw21I GWGCWC 1 cut(s) 714
Alw26I GTCTC 2 cut(s) 51, 159
AlwI GGATC 1 cut(s) 236
Ama87I CYCGRG 1 cut(s) 552
Aor13HI TCCGGA 1 cut(s) 620
AoxI GGCC 2 cut(s) 324, 455
ApeKI GCWGC 2 cut(s) 173, 344
ApoI RAATTY 1 cut(s) 191
Asp700I GAANNNNTTC 1 cut(s) 257
AspS9I GGNCC 2 cut(s) 324, 456
AsuHPI GGTGA 3 cut(s) 78, 456, 505
AsuII TTCGAA 2 cut(s) 665, 725
AvaI CYCGRG 1 cut(s) 552
AxyI CCTNAGG 1 cut(s) 423
BaeGI GKGCMC 1 cut(s) 559
BanII GRGCYC 2 cut(s) 63, 414
BbsI GAAGAC 1 cut(s) 367
Bbv12I GWGCWC 1 cut(s) 714
BbvI GCAGC 2 cut(s) 160, 356
BccI CCATC 1 cut(s) 356
BcoDI GTCTC 2 cut(s) 51, 159
BfaI CTAG 2 cut(s) 180, 396
BglI GCCNNNNNGGC 1 cut(s) 353
BisI GCNGC 2 cut(s) 174, 345
BlsI GCNGC 2 cut(s) 175, 346
BmeT110I CYCGRG 1 cut(s) 552
BmgT120I GGNCC 2 cut(s) 324, 456
BmsI GCATC 3 cut(s) 185, 292, 496
BpiI GAAGAC 1 cut(s) 367
BplI GAGNNNNNCTC 2 cut(s) 248, 280
Bpu14I TTCGAA 2 cut(s) 665, 725
BsaI GGTCTC 1 cut(s) 159
BsaJI CCNNGG 3 cut(s) 327, 571, 637
BsaWI WCCGGW 1 cut(s) 620
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse1I ACTGG 1 cut(s) 310
Bse21I CCTNAGG 1 cut(s) 423
BseAI TCCGGA 1 cut(s) 620
BseDI CCNNGG 3 cut(s) 327, 571, 637
BseGI GGATG 1 cut(s) 521
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMII CTCAG 4 cut(s) 104, 350, 593, 629
BseNI ACTGG 1 cut(s) 310
BseRI GAGGAG 1 cut(s) 408
BseSI GKGCMC 1 cut(s) 559
BseXI GCAGC 2 cut(s) 160, 356
BshFI GGCC 2 cut(s) 326, 457
BsiHKAI GWGCWC 1 cut(s) 714
BsiHKCI CYCGRG 1 cut(s) 552
BsiSI CCGG 2 cut(s) 487, 621
BslI CCNNNNNNNGG 1 cut(s) 225
BsmAI GTCTC 2 cut(s) 51, 159
BsnI GGCC 2 cut(s) 326, 457
Bso31I GGTCTC 1 cut(s) 159
BsoBI CYCGRG 1 cut(s) 552
Bsp119I TTCGAA 2 cut(s) 665, 725
Bsp1286I GDGCHC 4 cut(s) 63, 414, 559, 714
Bsp13I TCCGGA 1 cut(s) 620
Bsp143I GATC 1 cut(s) 228
BspACI CCGC 4 cut(s) 110, 525, 642, 705
BspANI GGCC 2 cut(s) 326, 457
BspCNI CTCAG 4 cut(s) 103, 351, 592, 628
BspEI TCCGGA 1 cut(s) 620
BspPI GGATC 1 cut(s) 236
BspQI GCTCTTC 3 cut(s) 327, 359, 488
BspT104I TTCGAA 2 cut(s) 665, 725
BspTNI GGTCTC 1 cut(s) 159
BsrI ACTGG 1 cut(s) 310
BssECI CCNNGG 3 cut(s) 327, 571, 637
BssMI GATC 1 cut(s) 228
BssT1I CCWWGG 3 cut(s) 327, 571, 637
Bst4CI ACNGT 2 cut(s) 205, 479
Bst6I CTCTTC 3 cut(s) 327, 359, 488
BstBI TTCGAA 2 cut(s) 665, 725
BstC8I GCNNGC 2 cut(s) 8, 132
BstDEI CTNAG 7 cut(s) 90, 123, 359, 423, 482, 579, 615
BstF5I GGATG 1 cut(s) 521
BstKTI GATC 1 cut(s) 231
BstMAI GTCTC 2 cut(s) 51, 159
BstMBI GATC 1 cut(s) 228
BstMWI GCNNNNNNNGC 2 cut(s) 296, 353
BstNSI RCATGY 1 cut(s) 134
BstSLI GKGCMC 1 cut(s) 559
BstV1I GCAGC 2 cut(s) 160, 356
BstV2I GAAGAC 1 cut(s) 367
BstX2I RGATCY 1 cut(s) 228
BstYI RGATCY 1 cut(s) 228
Bsu36I CCTNAGG 1 cut(s) 423
BsuRI GGCC 2 cut(s) 326, 457
BtgZI GCGATG 1 cut(s) 519
BtsCI GGATG 1 cut(s) 521
BtsIMutI CAGTG 2 cut(s) 317, 356
Cac8I GCNNGC 2 cut(s) 8, 132
Cfr13I GGNCC 2 cut(s) 324, 456
Csp6I GTAC 1 cut(s) 20
CviAII CATG 3 cut(s) 116, 131, 370
CviQI GTAC 1 cut(s) 20
DdeI CTNAG 7 cut(s) 90, 123, 359, 423, 482, 579, 615
DpnI GATC 1 cut(s) 230
DpnII GATC 1 cut(s) 228
Eam1104I CTCTTC 3 cut(s) 327, 359, 488
EarI CTCTTC 3 cut(s) 327, 359, 488
Eco130I CCWWGG 3 cut(s) 327, 571, 637
Eco24I GRGCYC 2 cut(s) 63, 414
Eco31I GGTCTC 1 cut(s) 159
Eco81I CCTNAGG 1 cut(s) 423
Eco88I CYCGRG 1 cut(s) 552
EcoT14I CCWWGG 3 cut(s) 327, 571, 637
EcoT38I GRGCYC 2 cut(s) 63, 414
ErhI CCWWGG 3 cut(s) 327, 571, 637
FaeI CATG 3 cut(s) 119, 134, 373
FaiI YATR 6 cut(s) 117, 132, 242, 293, 371, 501
FatI CATG 3 cut(s) 115, 130, 369
Fnu4HI GCNGC 2 cut(s) 174, 345
FokI GGATG 1 cut(s) 508
FriOI GRGCYC 2 cut(s) 63, 414
Fsp4HI GCNGC 2 cut(s) 174, 345
FspBI CTAG 2 cut(s) 180, 396
GluI GCNGC 2 cut(s) 174, 345
HaeIII GGCC 2 cut(s) 326, 457
HapII CCGG 2 cut(s) 487, 621
Hin1II CATG 3 cut(s) 119, 134, 373
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 4 cut(s) 37, 44, 590, 722
HpaII CCGG 2 cut(s) 487, 621
HphI GGTGA 3 cut(s) 78, 456, 505
Hpy166II GTNNAC 1 cut(s) 74
Hpy188I TCNGA 3 cut(s) 159, 582, 721
Hpy188III TCNNGA 5 cut(s) 28, 41, 99, 180, 621
Hpy8I GTNNAC 1 cut(s) 74
Hpy99I CGWCG 1 cut(s) 737
HpyAV CCTTC 2 cut(s) 577, 618
HpyCH4III ACNGT 2 cut(s) 205, 479
HpyCH4IV ACGT 3 cut(s) 221, 238, 562
HpyCH4V TGCA 3 cut(s) 176, 509, 648
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 353
HpyF3I CTNAG 7 cut(s) 90, 123, 359, 423, 482, 579, 615
HpySE526I ACGT 3 cut(s) 221, 238, 562
Hsp92II CATG 3 cut(s) 119, 134, 373
Kpn2I TCCGGA 1 cut(s) 620
Kzo9I GATC 1 cut(s) 228
LguI GCTCTTC 3 cut(s) 327, 359, 488
LmnI GCTCC 1 cut(s) 220
LpnPI CCDG 5 cut(s) 54, 84, 323, 500, 634
Lsp1109I GCAGC 2 cut(s) 160, 356
LweI GCATC 3 cut(s) 185, 292, 496
MaeI CTAG 2 cut(s) 180, 396
MaeII ACGT 3 cut(s) 221, 238, 562
MaeIII GTNAC 3 cut(s) 199, 462, 563
MalI GATC 1 cut(s) 230
MboI GATC 1 cut(s) 228
MboII GAAGA 8 cut(s) 152, 155, 344, 367, 376, 505, 599, 687
MflI RGATCY 1 cut(s) 228
MhlI GDGCHC 4 cut(s) 63, 414, 559, 714
MluCI AATT 1 cut(s) 191
MlyI GAGTC 2 cut(s) 46, 53
MmeI TCCRAC 2 cut(s) 187, 252
MroI TCCGGA 1 cut(s) 620
MroXI GAANNNNTTC 1 cut(s) 257
MseI TTAA 2 cut(s) 14, 754
MslI CAYNNNNRTG 1 cut(s) 135
MspI CCGG 2 cut(s) 487, 621
MwoI GCNNNNNNNGC 2 cut(s) 296, 353
NdeII GATC 1 cut(s) 228
NlaIII CATG 3 cut(s) 119, 134, 373
NmeAIII GCCGAG 2 cut(s) 433, 733
NmuCI GTSAC 2 cut(s) 462, 563
NspI RCATGY 1 cut(s) 134
NspV TTCGAA 2 cut(s) 665, 725
PaeI GCATGC 1 cut(s) 134
PciSI GCTCTTC 3 cut(s) 327, 359, 488
PcsI WCGNNNNNNNCGW 1 cut(s) 559
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 2 cut(s) 590, 722
PkrI GCNGC 2 cut(s) 175, 346
PleI GAGTC 2 cut(s) 45, 52
PpsI GAGTC 2 cut(s) 45, 52
PspPI GGNCC 2 cut(s) 324, 456
PsuI RGATCY 1 cut(s) 228
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
RseI CAYNNNNRTG 1 cut(s) 135
SapI GCTCTTC 3 cut(s) 327, 359, 488
SaqAI TTAA 2 cut(s) 14, 754
SatI GCNGC 2 cut(s) 174, 345
Sau3AI GATC 1 cut(s) 228
Sau96I GGNCC 2 cut(s) 324, 456
SchI GAGTC 2 cut(s) 46, 53
SduI GDGCHC 4 cut(s) 63, 414, 559, 714
SfaNI GCATC 3 cut(s) 185, 292, 496
SfuI TTCGAA 2 cut(s) 665, 725
SmiMI CAYNNNNRTG 1 cut(s) 135
SphI GCATGC 1 cut(s) 134
Sse9I AATT 1 cut(s) 191
SsiI CCGC 4 cut(s) 110, 525, 642, 705
SspMI CTAG 2 cut(s) 180, 396
StyI CCWWGG 3 cut(s) 327, 571, 637
TaaI ACNGT 2 cut(s) 205, 479
TaiI ACGT 3 cut(s) 224, 241, 565
TaqI TCGA 3 cut(s) 266, 665, 725
TasI AATT 1 cut(s) 191
TatI WGTACW 1 cut(s) 19
TfiI GAWTC 2 cut(s) 590, 722
Tru1I TTAA 2 cut(s) 14, 754
Tru9I TTAA 2 cut(s) 14, 754
TscAI CASTG 2 cut(s) 317, 363
TseFI GTSAC 2 cut(s) 462, 563
TseI GCWGC 2 cut(s) 173, 344
Tsp45I GTSAC 2 cut(s) 462, 563
TspDTI ATGAA 2 cut(s) 153, 391
TspGWI ACGGA 3 cut(s) 687, 701, 750
TspRI CASTG 2 cut(s) 317, 363
XapI RAATTY 1 cut(s) 191
XbaI TCTAGA 1 cut(s) 179
XceI RCATGY 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 257
XspI CTAG 2 cut(s) 180, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.