pycom05g04050

CHCH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0004697
Physical Location & Seq
Reverse (-)
4680399 .. 4683069
2671 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 597 bp
ATGAAGAAAATCACAGATAAAATAGGAAAAATTTATCTATTAGAACTTGTTTGGGCCCAGATTAATGGATTTGGGCCGAGTAAGGTTATAAGTTTGGCCATGATAGTTTTTCCCAGACGCTTTCTTCTTCAATTTGTTAAGCTCTCCCCGATATTCGCTGCCATGGCTCGCCGCTCAGGAGGTCGATCTGCCCGCCCAGCTCCCCGTCATGCACCTGCACGCAACCCTCCTCAGCCTGTTCGCCAAGCTCCTCCTCCAGCTCCAGCTCAGTCTGGAAGTGGTGGATCGATCCTTTCCGGAATTGGATCAACCATAGCACAGGGTATGGCTTTTGGTACTGGAAGTGCCGTGGCACACAGAGCTGTGGATGCTGTGATGGGTCCTCGCACTATTCAACATGAAACTGTAGTCTCTGAGGCCACTGCTGGTGCTCCAGTCCCCATGGCTAGCAGCATGGGTGGCTCAGATGCATGCAGTAATCACTCCAAGGCATTCCAAGATTGTGTCAACCACTTTGGAAGTGATATAAGCAAGTGCCAGTTCTACATGGATATGCTGTCCGAGTGCAAGAAGAACTCCGGCATGTTGAATGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CHCH

Protein Analysis

199

Amino Acids

20.84

Weight (kDa)

10.03

Isoelectric Point (pI)

53.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHCH PF06747 158 - 192 1.2e-07 CHCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 89
AarI CACCTGC 1 cut(s) 223
Acc36I ACCTGC 1 cut(s) 223
AccBSI CCGCTC 1 cut(s) 174
AccIII TCCGGA 1 cut(s) 296
AciI CCGC 2 cut(s) 172, 193
AclWI GGATC 3 cut(s) 283, 292, 313
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 1 cut(s) 30
AfaI GTAC 1 cut(s) 337
AgsI TTSAA 3 cut(s) 131, 395, 589
AluBI AGCT 6 cut(s) 142, 200, 248, 260, 266, 362
AluI AGCT 6 cut(s) 142, 200, 248, 260, 266, 362
Alw21I GWGCWC 1 cut(s) 433
Alw26I GTCTC 1 cut(s) 415
AlwI GGATC 3 cut(s) 283, 292, 313
Aor13HI TCCGGA 1 cut(s) 296
AoxI GGCC 4 cut(s) 54, 74, 96, 417
ApaI GGGCCC 1 cut(s) 58
ApeKI GCWGC 2 cut(s) 158, 450
ApoI RAATTY 1 cut(s) 30
AseI ATTAAT 1 cut(s) 63
AspS9I GGNCC 4 cut(s) 54, 55, 74, 380
AsuNHI GCTAGC 1 cut(s) 446
AvaII GGWCC 1 cut(s) 380
BaeGI GKGCMC 1 cut(s) 58
BalI TGGCCA 1 cut(s) 98
BanII GRGCYC 1 cut(s) 58
Bbv12I GWGCWC 1 cut(s) 433
BbvCI CCTCAGC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 145, 462
BccI CCATC 1 cut(s) 370
BceAI ACGGC 1 cut(s) 332
BcoDI GTCTC 1 cut(s) 415
BfaI CTAG 1 cut(s) 447
BfmI CTRYAG 1 cut(s) 405
BfuAI ACCTGC 1 cut(s) 223
BisI GCNGC 3 cut(s) 159, 172, 451
BlsI GCNGC 3 cut(s) 160, 173, 452
Bme18I GGWCC 1 cut(s) 380
BmgT120I GGNCC 4 cut(s) 54, 55, 74, 380
BmiI GGNNCC 2 cut(s) 56, 381
BmsI GCATC 2 cut(s) 358, 457
BmtI GCTAGC 1 cut(s) 450
BpmI CTGGAG 3 cut(s) 240, 246, 417
Bpu10I CCTNAGC 2 cut(s) 175, 231
Bsa29I ATCGAT 1 cut(s) 287
BsaJI CCNNGG 4 cut(s) 162, 348, 441, 486
BsaWI WCCGGW 1 cut(s) 296
Bse1I ACTGG 3 cut(s) 343, 434, 538
BseAI TCCGGA 1 cut(s) 296
BseCI ATCGAT 1 cut(s) 287
BseDI CCNNGG 4 cut(s) 162, 348, 441, 486
BseGI GGATG 1 cut(s) 373
BseMII CTCAG 5 cut(s) 189, 245, 281, 405, 477
BseNI ACTGG 3 cut(s) 343, 434, 538
BseRI GAGGAG 3 cut(s) 219, 240, 243
BseSI GKGCMC 1 cut(s) 58
BseXI GCAGC 2 cut(s) 145, 462
BseYI CCCAGC 1 cut(s) 196
BsgI GTGCAG 1 cut(s) 201
BshFI GGCC 4 cut(s) 56, 76, 98, 419
BshVI ATCGAT 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 433
BsiSI CCGG 2 cut(s) 297, 579
BslFI GGGAC 1 cut(s) 422
BsmAI GTCTC 1 cut(s) 415
BsmFI GGGAC 1 cut(s) 422
BsmI GAATGC 2 cut(s) 491, 595
BsnI GGCC 4 cut(s) 56, 76, 98, 419
Bsp120I GGGCCC 1 cut(s) 54
Bsp1286I GDGCHC 2 cut(s) 58, 433
Bsp13I TCCGGA 1 cut(s) 296
Bsp143I GATC 4 cut(s) 185, 284, 288, 305
Bsp19I CCATGG 2 cut(s) 162, 441
BspACI CCGC 2 cut(s) 172, 193
BspANI GGCC 4 cut(s) 56, 76, 98, 419
BspCNI CTCAG 5 cut(s) 188, 244, 280, 406, 476
BspDI ATCGAT 1 cut(s) 287
BspEI TCCGGA 1 cut(s) 296
BspLI GGNNCC 2 cut(s) 56, 381
BspMI ACCTGC 1 cut(s) 223
BspOI GCTAGC 1 cut(s) 450
BspPI GGATC 3 cut(s) 283, 292, 313
BsrBI CCGCTC 1 cut(s) 174
BsrI ACTGG 3 cut(s) 343, 434, 538
BssECI CCNNGG 4 cut(s) 162, 348, 441, 486
BssMI GATC 4 cut(s) 185, 284, 288, 305
BssT1I CCWWGG 3 cut(s) 162, 441, 486
Bst4CI ACNGT 1 cut(s) 406
BstC8I GCNNGC 5 cut(s) 169, 193, 220, 448, 472
BstDEI CTNAG 5 cut(s) 175, 231, 267, 414, 463
BstDSI CCRYGG 3 cut(s) 162, 348, 441
BstF5I GGATG 1 cut(s) 373
BstKTI GATC 4 cut(s) 188, 287, 291, 308
BstMAI GTCTC 1 cut(s) 415
BstMBI GATC 4 cut(s) 185, 284, 288, 305
BstMWI GCNNNNNNNGC 5 cut(s) 164, 197, 359, 368, 459
BstNSI RCATGY 2 cut(s) 474, 586
BstSFI CTRYAG 1 cut(s) 405
BstSLI GKGCMC 1 cut(s) 58
BstV1I GCAGC 2 cut(s) 145, 462
BstXI CCANNNNNNTGG 1 cut(s) 65
Bsu15I ATCGAT 1 cut(s) 287
BsuRI GGCC 4 cut(s) 56, 76, 98, 419
BsuTUI ATCGAT 1 cut(s) 287
BtgI CCRYGG 3 cut(s) 162, 348, 441
BtsCI GGATG 1 cut(s) 373
BtsI GCAGTG 1 cut(s) 420
BtsIMutI CAGTG 1 cut(s) 420
BveI ACCTGC 1 cut(s) 223
Cac8I GCNNGC 5 cut(s) 169, 193, 220, 448, 472
Cfr13I GGNCC 4 cut(s) 54, 55, 74, 380
ClaI ATCGAT 1 cut(s) 287
CseI GACGC 1 cut(s) 126
Csp6I GTAC 1 cut(s) 336
CviAII CATG 9 cut(s) 100, 163, 209, 398, 442, 454, 471, 547, 583
CviQI GTAC 1 cut(s) 336
DdeI CTNAG 5 cut(s) 175, 231, 267, 414, 463
DpnI GATC 4 cut(s) 187, 286, 290, 307
DpnII GATC 4 cut(s) 185, 284, 288, 305
EaeI YGGCCR 1 cut(s) 96
Eco130I CCWWGG 3 cut(s) 162, 441, 486
Eco24I GRGCYC 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 380
EcoO109I RGGNCCY 1 cut(s) 380
EcoT14I CCWWGG 3 cut(s) 162, 441, 486
EcoT22I ATGCAT 1 cut(s) 472
EcoT38I GRGCYC 1 cut(s) 58
ErhI CCWWGG 3 cut(s) 162, 441, 486
FaeI CATG 9 cut(s) 103, 166, 212, 401, 445, 457, 474, 550, 586
FaqI GGGAC 1 cut(s) 422
FatI CATG 9 cut(s) 99, 162, 208, 397, 441, 453, 470, 546, 582
FauI CCCGC 1 cut(s) 200
Fnu4HI GCNGC 3 cut(s) 159, 172, 451
FokI GGATG 1 cut(s) 380
FriOI GRGCYC 1 cut(s) 58
Fsp4HI GCNGC 3 cut(s) 159, 172, 451
FspBI CTAG 1 cut(s) 447
GluI GCNGC 3 cut(s) 159, 172, 451
GsaI CCCAGC 1 cut(s) 200
GsuI CTGGAG 3 cut(s) 240, 246, 417
HaeIII GGCC 4 cut(s) 56, 76, 98, 419
HapII CCGG 2 cut(s) 297, 579
HgaI GACGC 1 cut(s) 126
Hin1II CATG 9 cut(s) 103, 166, 212, 401, 445, 457, 474, 550, 586
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HpaII CCGG 2 cut(s) 297, 579
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 3 cut(s) 415, 466, 562
Hpy188III TCNNGA 3 cut(s) 177, 273, 297
Hpy8I GTNNAC 1 cut(s) 508
HpyCH4III ACNGT 1 cut(s) 406
HpyCH4V TGCA 5 cut(s) 212, 218, 470, 474, 567
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 197, 359, 368, 459
HpyF3I CTNAG 5 cut(s) 175, 231, 267, 414, 463
Hsp92II CATG 9 cut(s) 103, 166, 212, 401, 445, 457, 474, 550, 586
Kpn2I TCCGGA 1 cut(s) 296
Kzo9I GATC 4 cut(s) 185, 284, 288, 305
LmnI GCTCC 4 cut(s) 205, 253, 265, 436
Lsp1109I GCAGC 2 cut(s) 145, 462
LweI GCATC 2 cut(s) 358, 457
MaeI CTAG 1 cut(s) 447
MalI GATC 4 cut(s) 187, 286, 290, 307
MbiI CCGCTC 1 cut(s) 174
MboI GATC 4 cut(s) 185, 284, 288, 305
MboII GAAGA 4 cut(s) 16, 116, 119, 583
MhlI GDGCHC 2 cut(s) 58, 433
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 3 cut(s) 30, 131, 300
MluNI TGGCCA 1 cut(s) 98
MnlI CCTC 7 cut(s) 173, 237, 240, 261, 264, 393, 409
Mox20I TGGCCA 1 cut(s) 98
Mph1103I ATGCAT 1 cut(s) 472
MroI TCCGGA 1 cut(s) 296
MscI TGGCCA 1 cut(s) 98
MseI TTAA 2 cut(s) 63, 138
MslI CAYNNNNRTG 1 cut(s) 551
Msp20I TGGCCA 1 cut(s) 98
MspI CCGG 2 cut(s) 297, 579
Mva1269I GAATGC 2 cut(s) 491, 595
MwoI GCNNNNNNNGC 5 cut(s) 164, 197, 359, 368, 459
NcoI CCATGG 2 cut(s) 162, 441
NdeII GATC 4 cut(s) 185, 284, 288, 305
NheI GCTAGC 1 cut(s) 446
NlaIII CATG 9 cut(s) 103, 166, 212, 401, 445, 457, 474, 550, 586
NlaIV GGNNCC 2 cut(s) 56, 381
NmeAIII GCCGAG 1 cut(s) 102
NsiI ATGCAT 1 cut(s) 472
NspI RCATGY 2 cut(s) 474, 586
PaeI GCATGC 1 cut(s) 474
PaqCI CACCTGC 1 cut(s) 223
PctI GAATGC 2 cut(s) 491, 595
PkrI GCNGC 3 cut(s) 160, 173, 452
PpuMI RGGWCCY 1 cut(s) 380
PshBI ATTAAT 1 cut(s) 63
PsiI TTATAA 1 cut(s) 89
Psp5II RGGWCCY 1 cut(s) 380
PspFI CCCAGC 1 cut(s) 196
PspN4I GGNNCC 2 cut(s) 56, 381
PspOMI GGGCCC 1 cut(s) 54
PspPI GGNCC 4 cut(s) 54, 55, 74, 380
PspPPI RGGWCCY 1 cut(s) 380
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
RseI CAYNNNNRTG 1 cut(s) 551
SaqAI TTAA 2 cut(s) 63, 138
SatI GCNGC 3 cut(s) 159, 172, 451
Sau3AI GATC 4 cut(s) 185, 284, 288, 305
Sau96I GGNCC 4 cut(s) 54, 55, 74, 380
SduI GDGCHC 2 cut(s) 58, 433
SetI ASST 9 cut(s) 87, 144, 184, 202, 217, 250, 262, 268, 364
SfaNI GCATC 2 cut(s) 358, 457
SfcI CTRYAG 1 cut(s) 405
SinI GGWCC 1 cut(s) 380
SmiMI CAYNNNNRTG 1 cut(s) 551
SphI GCATGC 1 cut(s) 474
Sse9I AATT 3 cut(s) 30, 131, 300
SsiI CCGC 2 cut(s) 172, 193
SspMI CTAG 1 cut(s) 447
StyI CCWWGG 3 cut(s) 162, 441, 486
TaaI ACNGT 1 cut(s) 406
TaqI TCGA 2 cut(s) 184, 287
TasI AATT 3 cut(s) 30, 131, 300
TauI GCSGC 1 cut(s) 174
Tru1I TTAA 2 cut(s) 63, 138
Tru9I TTAA 2 cut(s) 63, 138
TscAI CASTG 1 cut(s) 427
TseI GCWGC 2 cut(s) 158, 450
TspDTI ATGAA 2 cut(s) 17, 414
TspRI CASTG 1 cut(s) 427
VpaK11BI GGWCC 1 cut(s) 380
VspI ATTAAT 1 cut(s) 63
XapI RAATTY 1 cut(s) 30
XceI RCATGY 2 cut(s) 474, 586
XspI CTAG 1 cut(s) 447
Zsp2I ATGCAT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.