pycom05g06570

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008704
Physical Location & Seq
Forward (+)
9091426 .. 9094079
2654 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 954 bp
ATGCAAGTGCTTGAGCTTGTAGGAAAGGAAACTTTGGATCTGCTGATCACAGAGACTAGTATAGAAGTCAATAAGAATTCTACACTTTCTGAAAAGGAAACCAGAGAGGATCAGTTATATGAGGAAGTGGGATTTGATCGATGCTTCTACATATATGGAGGTCCAGAACAGTTGGAGGAACTGGAGGGTTTGTCCAGCCATTATGCACTATTATTTAGTCGGAGAAAAGGAAAATTGTCATCAGAACAGAAGTCTGTTTATGATGGAAAGCTTAAAGAGGTGCAACAGATTTTCAGTCTGGATATTAAGTTGGATGGAAGTGGTGCAGAGTCGGACAAAGGCAAAAAGAAAGAGACCAGAGATGATGGAAATACTGATGAGATGAAAACTCTTCATGATTCAAGCGTCAGTAAGGCTGCTGATATGGCGGCTGGGTTCACAAGTGCCTTGGCAGGACAAGCTGTTAACGATATAATTCAAAGAACCACTGGAAGATTAGAGAGTCTTCACTCAGAGGGGGTTCATAGACTGTCTGAATTGTGCTGTTCTGCTGTATCTCAACTGCTGATTCTTGGTAAATCCATCATATCTAGTGCAAACAAAACTCAGGTAGAAGATGTGGATGCTGATCTGGAGAATATTGATTGGCCTGAAGATTCTGTTGAGAAAGCAAAGATAATCAGATCAAAAGCACAAGCAATGGCAGGATATGTGGAGGCAGTGCCTAGCAGTTTCGTCACAGGTATGTCTGATGTTGCCGAAGCTTATCTAGCAGCCATAAAGGGTGCTTCTGAGGAATCCCACGAAGAGCTCTCACAGACATCGATCCAAGAAAAAGCACGTACTTTCTCTAAACATCTCCATGATGACCAGACCACAGCTTTGAGCAAAATCCAGGATGGGTTACTCTACTTGTCGTACGTTGTCATTTCGACCTCTATGCCTGCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF719

Protein Analysis

318

Amino Acids

34.72

Weight (kDa)

4.79

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7798 PF25074 43 - 314 4.4e-137 Domain of unknown function (DUF7798)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 428
AclWI GGATC 3 cut(s) 45, 117, 820
AcsI RAATTY 1 cut(s) 76
AcuI CTGAAG 1 cut(s) 672
AfaI GTAC 2 cut(s) 844, 920
AgsI TTSAA 2 cut(s) 402, 479
AhlI ACTAGT 1 cut(s) 56
AjnI CCWGG 1 cut(s) 894
AluBI AGCT 6 cut(s) 16, 271, 461, 764, 811, 881
AluI AGCT 6 cut(s) 16, 271, 461, 764, 811, 881
Alw21I GWGCWC 1 cut(s) 813
Alw26I GTCTC 2 cut(s) 47, 347
AlwI GGATC 3 cut(s) 45, 117, 820
AoxI GGCC 1 cut(s) 647
ApeKI GCWGC 3 cut(s) 416, 773, 947
ApoI RAATTY 1 cut(s) 76
ArsI GACNNNNNNTTYG 2 cut(s) 865, 897
AspS9I GGNCC 1 cut(s) 161
AvaII GGWCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 813
BbsI GAAGAC 1 cut(s) 497
Bbv12I GWGCWC 1 cut(s) 813
BbvI GCAGC 2 cut(s) 403, 785
BccI CCATC 5 cut(s) 257, 308, 359, 590, 893
BcgI CGANNNNNNTGC 1 cut(s) 922
BciT130I CCWGG 1 cut(s) 896
BclI TGATCA 1 cut(s) 45
BcoDI GTCTC 2 cut(s) 47, 347
BcuI ACTAGT 1 cut(s) 56
BfaI CTAG 4 cut(s) 57, 591, 726, 770
BfmI CTRYAG 1 cut(s) 945
BisI GCNGC 4 cut(s) 417, 429, 774, 948
BlsI GCNGC 4 cut(s) 418, 430, 775, 949
Bme1390I CCNGG 1 cut(s) 896
Bme18I GGWCC 1 cut(s) 161
BmgT120I GGNCC 1 cut(s) 161
BmrFI CCNGG 1 cut(s) 896
BmsI GCATC 2 cut(s) 131, 613
BpiI GAAGAC 1 cut(s) 497
BpmI CTGGAG 2 cut(s) 203, 653
BpuEI CTTGAG 1 cut(s) 32
Bsa29I ATCGAT 2 cut(s) 139, 824
BsaAI YACGTR 1 cut(s) 842
BsaBI GATNNNNATC 1 cut(s) 627
BsaI GGTCTC 1 cut(s) 347
BsaJI CCNNGG 1 cut(s) 447
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bse1I ACTGG 2 cut(s) 186, 493
Bse3DI GCAATG 1 cut(s) 705
Bse8I GATNNNNATC 1 cut(s) 627
BseBI CCWGG 1 cut(s) 896
BseCI ATCGAT 2 cut(s) 139, 824
BseDI CCNNGG 1 cut(s) 447
BseGI GGATG 3 cut(s) 319, 628, 904
BseJI GATNNNNATC 1 cut(s) 627
BseMI GCAATG 1 cut(s) 705
BseMII CTCAG 3 cut(s) 525, 620, 783
BseNI ACTGG 2 cut(s) 186, 493
BseXI GCAGC 2 cut(s) 403, 785
BseYI CCCAGC 1 cut(s) 431
BsgI GTGCAG 1 cut(s) 345
BshFI GGCC 1 cut(s) 649
BshVI ATCGAT 2 cut(s) 139, 824
BsiHKAI GWGCWC 1 cut(s) 813
BsiWI CGTACG 1 cut(s) 918
BsmAI GTCTC 2 cut(s) 47, 347
BsnI GGCC 1 cut(s) 649
Bso31I GGTCTC 1 cut(s) 347
Bsp1286I GDGCHC 1 cut(s) 813
Bsp143I GATC 7 cut(s) 37, 45, 109, 136, 628, 683, 825
BspACI CCGC 1 cut(s) 428
BspANI GGCC 1 cut(s) 649
BspCNI CTCAG 3 cut(s) 524, 619, 784
BspDI ATCGAT 2 cut(s) 139, 824
BspHI TCATGA 1 cut(s) 394
BspMAI CTGCAG 1 cut(s) 949
BspPI GGATC 3 cut(s) 45, 117, 820
BspQI GCTCTTC 1 cut(s) 801
BspTNI GGTCTC 1 cut(s) 347
BsrDI GCAATG 1 cut(s) 705
BsrI ACTGG 2 cut(s) 186, 493
BssECI CCNNGG 1 cut(s) 447
BssMI GATC 7 cut(s) 37, 45, 109, 136, 628, 683, 825
BssT1I CCWWGG 1 cut(s) 447
Bst2UI CCWGG 1 cut(s) 896
Bst4CI ACNGT 2 cut(s) 171, 531
Bst6I CTCTTC 2 cut(s) 396, 801
BstBAI YACGTR 1 cut(s) 842
BstC8I GCNNGC 1 cut(s) 945
BstDEI CTNAG 3 cut(s) 511, 606, 792
BstF5I GGATG 3 cut(s) 319, 628, 904
BstKTI GATC 7 cut(s) 40, 48, 112, 139, 631, 686, 828
BstMAI GTCTC 2 cut(s) 47, 347
BstMBI GATC 7 cut(s) 37, 45, 109, 136, 628, 683, 825
BstMWI GCNNNNNNNGC 3 cut(s) 425, 458, 770
BstNI CCWGG 1 cut(s) 896
BstSCI CCNGG 1 cut(s) 894
BstSFI CTRYAG 1 cut(s) 945
BstV1I GCAGC 2 cut(s) 403, 785
BstV2I GAAGAC 1 cut(s) 497
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
Bsu15I ATCGAT 2 cut(s) 139, 824
BsuRI GGCC 1 cut(s) 649
BsuTUI ATCGAT 2 cut(s) 139, 824
BtsCI GGATG 3 cut(s) 319, 628, 904
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 2 cut(s) 486, 726
Cac8I GCNNGC 1 cut(s) 945
CciI TCATGA 1 cut(s) 394
Cfr13I GGNCC 1 cut(s) 161
ClaI ATCGAT 2 cut(s) 139, 824
CseI GACGC 1 cut(s) 394
Csp6I GTAC 2 cut(s) 843, 919
CviAII CATG 3 cut(s) 395, 863, 951
CviQI GTAC 2 cut(s) 843, 919
DdeI CTNAG 3 cut(s) 511, 606, 792
DpnI GATC 7 cut(s) 39, 47, 111, 138, 630, 685, 827
DpnII GATC 7 cut(s) 37, 45, 109, 136, 628, 683, 825
Eam1104I CTCTTC 2 cut(s) 396, 801
EarI CTCTTC 2 cut(s) 396, 801
Ecl136II GAGCTC 1 cut(s) 811
Eco130I CCWWGG 1 cut(s) 447
Eco24I GRGCYC 1 cut(s) 813
Eco31I GGTCTC 1 cut(s) 347
Eco47I GGWCC 1 cut(s) 161
Eco53kI GAGCTC 1 cut(s) 811
Eco57I CTGAAG 1 cut(s) 672
EcoICRI GAGCTC 1 cut(s) 811
EcoRI GAATTC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 894
EcoT14I CCWWGG 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 813
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 3 cut(s) 398, 866, 954
FatI CATG 3 cut(s) 394, 862, 950
FbaI TGATCA 1 cut(s) 45
Fnu4HI GCNGC 4 cut(s) 417, 429, 774, 948
FokI GGATG 3 cut(s) 326, 635, 911
FriOI GRGCYC 1 cut(s) 813
Fsp4HI GCNGC 4 cut(s) 417, 429, 774, 948
FspBI CTAG 4 cut(s) 57, 591, 726, 770
GluI GCNGC 4 cut(s) 417, 429, 774, 948
GsaI CCCAGC 1 cut(s) 435
GsuI CTGGAG 2 cut(s) 203, 653
HaeIII GGCC 1 cut(s) 649
HgaI GACGC 1 cut(s) 394
Hin1II CATG 3 cut(s) 398, 866, 954
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HindIII AAGCTT 2 cut(s) 269, 762
HinfI GANTC 6 cut(s) 329, 398, 502, 568, 656, 797
HpaI GTTAAC 1 cut(s) 466
Hpy166II GTNNAC 2 cut(s) 438, 466
Hpy188I TCNGA 9 cut(s) 91, 222, 244, 334, 514, 535, 683, 751, 793
Hpy188III TCNNGA 4 cut(s) 164, 299, 395, 632
Hpy8I GTNNAC 2 cut(s) 438, 466
HpyCH4III ACNGT 2 cut(s) 171, 531
HpyCH4IV ACGT 2 cut(s) 841, 921
HpyCH4V TGCA 6 cut(s) 4, 206, 283, 326, 596, 947
HpyF10VI GCNNNNNNNGC 3 cut(s) 425, 458, 770
HpyF3I CTNAG 3 cut(s) 511, 606, 792
HpySE526I ACGT 2 cut(s) 841, 921
Hsp92II CATG 3 cut(s) 398, 866, 954
Ksp22I TGATCA 1 cut(s) 45
KspAI GTTAAC 1 cut(s) 466
Kzo9I GATC 7 cut(s) 37, 45, 109, 136, 628, 683, 825
LguI GCTCTTC 1 cut(s) 801
Lsp1109I GCAGC 2 cut(s) 403, 785
LweI GCATC 2 cut(s) 131, 613
MaeI CTAG 4 cut(s) 57, 591, 726, 770
MaeII ACGT 2 cut(s) 841, 921
MaeIII GTNAC 2 cut(s) 736, 903
MalI GATC 7 cut(s) 39, 47, 111, 138, 630, 685, 827
MboI GATC 7 cut(s) 37, 45, 109, 136, 628, 683, 825
MboII GAAGA 6 cut(s) 383, 497, 504, 626, 665, 818
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 813
MluCI AATT 4 cut(s) 76, 233, 474, 536
MlyI GAGTC 2 cut(s) 338, 511
MmeI TCCRAC 4 cut(s) 153, 200, 291, 312
MseI TTAA 3 cut(s) 273, 306, 465
MslI CAYNNNNRTG 2 cut(s) 743, 861
MspR9I CCNGG 1 cut(s) 896
MvaI CCWGG 1 cut(s) 896
MwoI GCNNNNNNNGC 3 cut(s) 425, 458, 770
NdeII GATC 7 cut(s) 37, 45, 109, 136, 628, 683, 825
NlaIII CATG 3 cut(s) 398, 866, 954
NmuCI GTSAC 1 cut(s) 736
PagI TCATGA 1 cut(s) 394
PciSI GCTCTTC 1 cut(s) 801
PfeI GAWTC 4 cut(s) 398, 568, 656, 797
Pfl23II CGTACG 1 cut(s) 918
PfoI TCCNGGA 1 cut(s) 894
PkrI GCNGC 4 cut(s) 418, 430, 775, 949
PleI GAGTC 2 cut(s) 337, 510
PpsI GAGTC 2 cut(s) 337, 510
Ppu21I YACGTR 1 cut(s) 842
Psp124BI GAGCTC 1 cut(s) 813
Psp6I CCWGG 1 cut(s) 894
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 1 cut(s) 894
PspLI CGTACG 1 cut(s) 918
PspPI GGNCC 1 cut(s) 161
PstI CTGCAG 1 cut(s) 949
PsuI RGATCY 1 cut(s) 37
RsaI GTAC 2 cut(s) 844, 920
RsaNI GTAC 2 cut(s) 843, 919
RseI CAYNNNNRTG 2 cut(s) 743, 861
SacI GAGCTC 1 cut(s) 813
SapI GCTCTTC 1 cut(s) 801
SaqAI TTAA 3 cut(s) 273, 306, 465
SatI GCNGC 4 cut(s) 417, 429, 774, 948
Sau3AI GATC 7 cut(s) 37, 45, 109, 136, 628, 683, 825
Sau96I GGNCC 1 cut(s) 161
SchI GAGTC 2 cut(s) 338, 511
ScrFI CCNGG 1 cut(s) 896
SduI GDGCHC 1 cut(s) 813
SfaNI GCATC 2 cut(s) 131, 613
SfcI CTRYAG 1 cut(s) 945
SinI GGWCC 1 cut(s) 161
SmiMI CAYNNNNRTG 2 cut(s) 743, 861
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SpeI ACTAGT 1 cut(s) 56
Sse9I AATT 4 cut(s) 76, 233, 474, 536
SsiI CCGC 1 cut(s) 428
SspI AATATT 1 cut(s) 640
SspMI CTAG 4 cut(s) 57, 591, 726, 770
SstI GAGCTC 1 cut(s) 813
StyD4I CCNGG 1 cut(s) 894
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 2 cut(s) 171, 531
TaiI ACGT 2 cut(s) 844, 924
TaqI TCGA 3 cut(s) 139, 824, 932
TasI AATT 4 cut(s) 76, 233, 474, 536
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 4 cut(s) 398, 568, 656, 797
Tru1I TTAA 3 cut(s) 273, 306, 465
Tru9I TTAA 3 cut(s) 273, 306, 465
TscAI CASTG 2 cut(s) 493, 726
TseFI GTSAC 1 cut(s) 736
TseI GCWGC 3 cut(s) 416, 773, 947
Tsp45I GTSAC 1 cut(s) 736
TspDTI ATGAA 3 cut(s) 383, 398, 512
TspRI CASTG 2 cut(s) 493, 726
VpaK11BI GGWCC 1 cut(s) 161
XapI RAATTY 1 cut(s) 76
XspI CTAG 4 cut(s) 57, 591, 726, 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.