pycom05g08030

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002668
Physical Location & Seq
Reverse (-)
10781895 .. 10786191
4297 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2082 bp
ATGCAAGGTCAAGGGAGTGGTATTGATACTTTCCTTGAAATCACAGATGTTGGGCTTGGATCTCTCTCCAACAGCACTAGTATGAATCAGCAGACTTCTTTAAATAATATGCTAAATCCTGTTGAAAACCAGTTGTCAAGCTATATGATGACATCTGGTGAGATTCCGTGTTTAAATGCCTCTAGTAATGACACCCAGAACTTGAGTGGTTCAGGTGAACCTAGCTCAAGATTGAATCTACGAAACCCAGTGGACGATGATGGGATGAAAATGGAAGAAGGTTGGTCATCTCCGTATAGTGCTTGTCCTGTTATTGGTCAAAGTTCAGAAGAAAGGCGAGTTGAACAAAATATCCTTTTTCCTGGGAGAGCTCGTATTGGACAAAGTGGCAATCAGATTCAAGGCGGGCCTTCTTTTTCTCAGGACTCTCAAAATGCAAATATAAATTCTGCGTATGTGGGAAATAGTGGTAATAGTGCGCCTAGCATGGAAGCTGATATGTCAGGTGGATTAGAAACAGAACAGACATCTTCTGGCAGTGTTTCGCCTGATATTATTGGGACTTCATCCAGAAGCACTGATTTTCTGGTTGAGGAAAATGATGGTAATGCAGGTTCTTCTTTTGGAACTTGGGGCTTATCGTGCAAGCGAAAGGCCCTTGAGGGTACTTCAGGCCAGGGTTATTCTGGTGGAAGTTCTAGCTCCCTTCCACAAGCTGAAACTAGTGCATGGAATACTGGTCCTGCTCATTACGGTCCTTCTAGTAATTTAAATTTATCTACACCCTTGCGTAGTTCTCCTAGTGGGTGTCCTTCAGAACAAAACTCAAGAACCAGGGTTGGTTTGAGACTTGGGACATCTGATACCTTTCCTTCATTAGGTGCAACAGGAAATGGAGCAAGCTCACTGCGGGATTTTTTTTGTAGGGGGGGTTGTCCTGGGCCTCAGCAAGAATCTGCTCCATCCAACTTATCATCACAAGGGAGATCAACAACTGCAGCTGCAACAAATTTGGTTGGTCCCCAAAGCCAGTTTCCTGCTATGCATATGAGTGGTTCGTCAGTAAACATGCTTCCTTTTCCTTGGACTGGTGCTTCCAGTTCAAGAGTGGGCAGCTTATCAAGTTCTCTTATGTCTGGATATAGAGGTGCTGAGTTAAGAGAAGAATCAAACTTAAGAAGCATTCCCAGAAACATTGTGGAGCATTCGATGTTTGCAACTGCTACTGATATGAGAAATTCAGCACAGGATCCCACAATTTGGAGTCTGGCCCCTGGAGAATTTAGCTCTTCTGTAGGTGCTCCTTCCTCTTCAACAATTGGCTCCAGTTCGGCCATTCGTTTTGTGCCTACTCCTGCATTGATCCGTGCCAATAACCCTCCTACCGAAAATCATCAAAGAACAATAGATTGGTCTTTGTTTCCTTCTGTTGATTCTCAACCCGGAGGTCATAGTCATTATAACCCAGTACCTTCAGGACCTGCTTCCTCACAAGATTCAGGAAGTTCTTCTCGATCTAATAACCAGGTTCGTCATCTACCACATCCTAGGTCAGCATTCTTGCTGGACAGACAAGGTGATGATGCTCTTAATATGCCCCAGTCACTGCGGGTATTAGCAGCCGACATCGAAGGGAGGCGCCGACTTATATCTGAGATTCGTCAAGTTTTGAATGCTATGCGCAGGGGTGAGAACTTACGCGTCGAGGATTATATGCTCTTTGACCCATTCATATATCATGGCATGGCTGAGATGCATGACAGGCACAGAGACATGCGCCTCGATGTTGATAACATGTCTTACGAGGAACTGCTGGCATTGGGGGAACGCATAGGAGATGTTAGCACTGGACTGAGTGAGGAGACTATTATGAAGTGCATGAAACAGCGAAAATTTCTTGCTATTTCAGAAGAATCCCCAGCAGATATGGAACCGTGCTGTGTCTGTCAGGAGGTATATGCTGATGAGGATGATATCGGCACACTAGATTGTGGGCACGACTTCCACACCAGTTGCATCAAACAATGGCTAATGCAGAAGAATCTGTGCCCGATTTGTAAAACAACAGCCCTGCTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006928 GO:0006935 GO:0007275 GO:0007399 GO:0007409 GO:0007411 GO:0007568 GO:0008045 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010228 GO:0010259 GO:0010468 GO:0010498 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0021675 GO:0022008 GO:0022414 GO:0023051 GO:0023056 GO:0030030 GO:0030154 GO:0030163 GO:0030182 GO:0030510 GO:0030513 GO:0031175 GO:0031323 GO:0031326 GO:0032446 GO:0032501 GO:0032502 GO:0032989 GO:0032990 GO:0032991 GO:0035107 GO:0035108 GO:0035136 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048468 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048736 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051603 GO:0060173 GO:0060255 GO:0060384 GO:0061061 GO:0061458 GO:0061564 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090092 GO:0090100 GO:0090287 GO:0097485 GO:0120036 GO:0120039 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

694

Amino Acids

74.18

Weight (kDa)

5.0

Isoelectric Point (pI)

66.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 643 - 687 3.7e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 646 - 687 4.5e-11 Ring finger domain
zf-C3HC4_2 PF13923 646 - 686 1.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 646 - 686 1.3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1461
Acc16I TGCGCA 1 cut(s) 1682
Acc36I ACCTGC 2 cut(s) 602, 1489
AccB1I GGYRCC 1 cut(s) 1638
AccB7I CCANNNNNTGG 1 cut(s) 1260
AccII CGCG 1 cut(s) 1701
AciI CCGC 3 cut(s) 405, 910, 1609
AclWI GGATC 4 cut(s) 67, 1244, 1257, 1357
AcoI YGGCCR 1 cut(s) 1332
AcsI RAATTY 6 cut(s) 445, 772, 1009, 1237, 1280, 1892
AcuI CTGAAG 3 cut(s) 654, 798, 1458
AcyI GRCGYC 1 cut(s) 1639
AfaI GTAC 2 cut(s) 667, 1470
AfiI CCNNNNNNNGG 4 cut(s) 314, 878, 1088, 1260
AflII CTTAAG 1 cut(s) 1174
AflIII ACRYGT 2 cut(s) 1699, 1794
AgsI TTSAA 8 cut(s) 38, 125, 235, 344, 401, 1104, 1314, 1672
AhlI ACTAGT 2 cut(s) 77, 722
AjnI CCWGG 6 cut(s) 361, 675, 833, 937, 1273, 1524
AjuI GAANNNNNNNTTGG 4 cut(s) 1017, 1049, 1393, 1425
AloI GAACNNNNNNTCC 2 cut(s) 336, 368
Alw21I GWGCWC 2 cut(s) 373, 1303
Alw26I GTCTC 3 cut(s) 841, 1764, 1856
AlwI GGATC 4 cut(s) 67, 1244, 1257, 1357
AlwNI CAGNNNCTG 2 cut(s) 1481, 1606
AoxI GGCC 6 cut(s) 407, 654, 673, 941, 1269, 1332
ApeKI GCWGC 4 cut(s) 998, 1001, 1113, 1619
ApoI RAATTY 6 cut(s) 445, 772, 1009, 1237, 1280, 1892
Asp700I GAANNNNTTC 1 cut(s) 1507
AspA2I CCTAGG 1 cut(s) 1547
AspLEI GCGC 4 cut(s) 481, 1641, 1683, 1779
AspS9I GGNCC 8 cut(s) 407, 655, 740, 755, 941, 1019, 1270, 1478
AsuC2I CCSGG 1 cut(s) 1443
AsuHPI GGTGA 4 cut(s) 170, 227, 1589, 1700
AvaII GGWCC 4 cut(s) 740, 755, 1019, 1478
AvrII CCTAGG 1 cut(s) 1547
BaeGI GKGCMC 2 cut(s) 1998, 2051
BamHI GGATCC 1 cut(s) 1249
BanI GGYRCC 1 cut(s) 1638
BanII GRGCYC 1 cut(s) 373
BarI GAAGNNNNNNTAC 2 cut(s) 1056, 1088
Bbv12I GWGCWC 2 cut(s) 373, 1303
BbvCI CCTCAGC 1 cut(s) 945
BbvI GCAGC 4 cut(s) 988, 1010, 1125, 1631
BccI CCATC 3 cut(s) 254, 596, 970
BciT130I CCWGG 6 cut(s) 363, 677, 835, 939, 1275, 1526
BcnI CCSGG 1 cut(s) 1443
BcoDI GTCTC 3 cut(s) 841, 1764, 1856
BcuI ACTAGT 2 cut(s) 77, 722
BfmI CTRYAG 2 cut(s) 996, 1293
BfoI RGCGCY 1 cut(s) 1642
BfrI CTTAAG 1 cut(s) 1174
BfuAI ACCTGC 2 cut(s) 602, 1489
BisI GCNGC 4 cut(s) 999, 1002, 1114, 1620
BlnI CCTAGG 1 cut(s) 1547
BlsI GCNGC 4 cut(s) 1000, 1003, 1115, 1621
Bme1390I CCNGG 7 cut(s) 363, 677, 835, 939, 1275, 1443, 1526
Bme18I GGWCC 4 cut(s) 740, 755, 1019, 1478
BmgT120I GGNCC 8 cut(s) 407, 655, 740, 755, 941, 1019, 1270, 1478
BmiI GGNNCC 6 cut(s) 1021, 1251, 1272, 1324, 1640, 1932
BmrFI CCNGG 7 cut(s) 363, 677, 835, 939, 1275, 1443, 1526
BmrI ACTGGG 3 cut(s) 242, 1460, 1594
BmsI GCATC 3 cut(s) 1573, 1743, 2025
BmuI ACTGGG 3 cut(s) 242, 1460, 1594
BpmI CTGGAG 2 cut(s) 1296, 1309
Bpu10I CCTNAGC 1 cut(s) 945
BpuEI CTTGAG 4 cut(s) 211, 223, 680, 811
BpuMI CCSGG 1 cut(s) 1443
BsaHI GRCGYC 1 cut(s) 1639
BsaJI CCNNGG 7 cut(s) 362, 676, 834, 938, 1082, 1273, 1547
BsaXI ACNNNNNCTCC 2 cut(s) 358, 388
Bsc4I CCNNNNNNNGG 4 cut(s) 314, 878, 1088, 1260
BseBI CCWGG 6 cut(s) 363, 677, 835, 939, 1275, 1526
BseDI CCNNGG 7 cut(s) 362, 676, 834, 938, 1082, 1273, 1547
BseGI GGATG 5 cut(s) 270, 566, 962, 1543, 1975
BseLI CCNNNNNNNGG 4 cut(s) 314, 878, 1088, 1260
BseMII CTCAG 6 cut(s) 434, 959, 1143, 1644, 1740, 1844
BseRI GAGGAG 1 cut(s) 1874
BseSI GKGCMC 2 cut(s) 1998, 2051
BseXI GCAGC 4 cut(s) 988, 1010, 1125, 1631
BseYI CCCAGC 1 cut(s) 1918
Bsh1236I CGCG 1 cut(s) 1701
BshFI GGCC 6 cut(s) 409, 656, 675, 943, 1271, 1334
BshNI GGYRCC 1 cut(s) 1638
BsiHKAI GWGCWC 2 cut(s) 373, 1303
BsiSI CCGG 1 cut(s) 1443
BslFI GGGAC 3 cut(s) 574, 868, 1005
BslI CCNNNNNNNGG 4 cut(s) 314, 878, 1088, 1260
BsmAI GTCTC 3 cut(s) 841, 1764, 1856
BsmFI GGGAC 3 cut(s) 574, 868, 1005
BsmI GAATGC 4 cut(s) 1182, 1204, 1556, 1678
BsnI GGCC 6 cut(s) 409, 656, 675, 943, 1271, 1334
Bsp1286I GDGCHC 4 cut(s) 373, 1303, 1998, 2051
Bsp143I GATC 5 cut(s) 59, 986, 1249, 1362, 1514
BspACI CCGC 3 cut(s) 405, 910, 1609
BspANI GGCC 6 cut(s) 409, 656, 675, 943, 1271, 1334
BspCNI CTCAG 6 cut(s) 433, 958, 1144, 1645, 1741, 1845
BspFNI CGCG 1 cut(s) 1701
BspLI GGNNCC 6 cut(s) 1021, 1251, 1272, 1324, 1640, 1932
BspMAI CTGCAG 1 cut(s) 1000
BspMI ACCTGC 2 cut(s) 602, 1489
BspPI GGATC 4 cut(s) 67, 1244, 1257, 1357
BspQI GCTCTTC 1 cut(s) 1294
BspT107I GGYRCC 1 cut(s) 1638
BspTI CTTAAG 1 cut(s) 1174
BssECI CCNNGG 7 cut(s) 362, 676, 834, 938, 1082, 1273, 1547
BssMI GATC 5 cut(s) 59, 986, 1249, 1362, 1514
BssNI GRCGYC 1 cut(s) 1639
BssT1I CCWWGG 2 cut(s) 1082, 1547
Bst2UI CCWGG 6 cut(s) 363, 677, 835, 939, 1275, 1526
Bst4CI ACNGT 2 cut(s) 755, 1935
Bst6I CTCTTC 2 cut(s) 1294, 1315
BstACI GRCGYC 1 cut(s) 1639
BstAFI CTTAAG 1 cut(s) 1174
BstC8I GCNNGC 4 cut(s) 407, 647, 901, 1815
BstDEI CTNAG 6 cut(s) 420, 945, 1152, 1653, 1749, 1853
BstENI CCTNNNNNAGG 1 cut(s) 876
BstF5I GGATG 5 cut(s) 270, 566, 962, 1543, 1975
BstFNI CGCG 1 cut(s) 1701
BstH2I RGCGCY 1 cut(s) 1642
BstHHI GCGC 4 cut(s) 481, 1641, 1683, 1779
BstKTI GATC 5 cut(s) 62, 989, 1252, 1365, 1517
BstMAI GTCTC 3 cut(s) 841, 1764, 1856
BstMBI GATC 5 cut(s) 59, 986, 1249, 1362, 1514
BstMWI GCNNNNNNNGC 2 cut(s) 642, 1762
BstNI CCWGG 6 cut(s) 363, 677, 835, 939, 1275, 1526
BstNSI RCATGY 3 cut(s) 1072, 1777, 1798
BstSCI CCNGG 7 cut(s) 361, 675, 833, 937, 1273, 1441, 1524
BstSFI CTRYAG 2 cut(s) 996, 1293
BstSLI GKGCMC 2 cut(s) 1998, 2051
BstUI CGCG 1 cut(s) 1701
BstV1I GCAGC 4 cut(s) 988, 1010, 1125, 1631
BstX2I RGATCY 2 cut(s) 59, 1249
BstYI RGATCY 2 cut(s) 59, 1249
BsuRI GGCC 6 cut(s) 409, 656, 675, 943, 1271, 1334
BtsCI GGATG 5 cut(s) 270, 566, 962, 1543, 1975
BtsI GCAGTG 3 cut(s) 544, 905, 1604
BtsIMutI CAGTG 6 cut(s) 255, 544, 576, 905, 1604, 1845
BveI ACCTGC 2 cut(s) 602, 1489
Cac8I GCNNGC 4 cut(s) 407, 647, 901, 1815
CaiI CAGNNNCTG 2 cut(s) 1481, 1606
CfoI GCGC 4 cut(s) 481, 1641, 1683, 1779
Cfr13I GGNCC 8 cut(s) 407, 655, 740, 755, 941, 1019, 1270, 1478
CseI GACGC 1 cut(s) 1690
CsiI ACCWGGT 1 cut(s) 1524
Csp6I GTAC 2 cut(s) 666, 1469
CspCI CAANNNNNGTGG 2 cut(s) 1994, 2029
CviAII CATG 9 cut(s) 487, 729, 1069, 1739, 1744, 1757, 1774, 1795, 1879
CviQI GTAC 2 cut(s) 666, 1469
DdeI CTNAG 6 cut(s) 420, 945, 1152, 1653, 1749, 1853
DinI GGCGCC 1 cut(s) 1640
DpnI GATC 5 cut(s) 61, 988, 1251, 1364, 1516
DpnII GATC 5 cut(s) 59, 986, 1249, 1362, 1514
DraI TTTAAA 3 cut(s) 102, 174, 771
EaeI YGGCCR 1 cut(s) 1332
Eam1104I CTCTTC 2 cut(s) 1294, 1315
EarI CTCTTC 2 cut(s) 1294, 1315
Ecl136II GAGCTC 1 cut(s) 371
Eco130I CCWWGG 2 cut(s) 1082, 1547
Eco24I GRGCYC 1 cut(s) 373
Eco32I GATATC 1 cut(s) 1975
Eco47I GGWCC 4 cut(s) 740, 755, 1019, 1478
Eco53kI GAGCTC 1 cut(s) 371
Eco57I CTGAAG 3 cut(s) 654, 798, 1458
EcoICRI GAGCTC 1 cut(s) 371
EcoNI CCTNNNNNAGG 1 cut(s) 876
EcoO109I RGGNCCY 2 cut(s) 655, 1478
EcoRII CCWGG 6 cut(s) 361, 675, 833, 937, 1273, 1524
EcoRV GATATC 1 cut(s) 1975
EcoT14I CCWWGG 2 cut(s) 1082, 1547
EcoT22I ATGCAT 2 cut(s) 1047, 1758
EcoT38I GRGCYC 1 cut(s) 373
EgeI GGCGCC 1 cut(s) 1640
EheI GGCGCC 1 cut(s) 1640
ErhI CCWWGG 2 cut(s) 1082, 1547
FaeI CATG 9 cut(s) 490, 732, 1072, 1742, 1747, 1760, 1777, 1798, 1882
FaqI GGGAC 3 cut(s) 574, 868, 1005
FatI CATG 9 cut(s) 486, 728, 1068, 1738, 1743, 1756, 1773, 1794, 1878
FauI CCCGC 3 cut(s) 398, 903, 1602
FauNDI CATATG 1 cut(s) 1047
Fnu4HI GCNGC 4 cut(s) 999, 1002, 1114, 1620
FokI GGATG 5 cut(s) 277, 553, 949, 1530, 1982
FriOI GRGCYC 1 cut(s) 373
Fsp4HI GCNGC 4 cut(s) 999, 1002, 1114, 1620
FspI TGCGCA 1 cut(s) 1682
GlaI GCGC 4 cut(s) 480, 1640, 1682, 1778
GluI GCNGC 4 cut(s) 999, 1002, 1114, 1620
GsaI CCCAGC 1 cut(s) 1922
GsuI CTGGAG 2 cut(s) 1296, 1309
HaeII RGCGCY 1 cut(s) 1642
HaeIII GGCC 6 cut(s) 409, 656, 675, 943, 1271, 1334
HapII CCGG 1 cut(s) 1443
HgaI GACGC 1 cut(s) 1690
HhaI GCGC 4 cut(s) 481, 1641, 1683, 1779
Hin1I GRCGYC 1 cut(s) 1639
Hin1II CATG 9 cut(s) 490, 732, 1072, 1742, 1747, 1760, 1777, 1798, 1882
Hin6I GCGC 4 cut(s) 479, 1639, 1681, 1777
HinP1I GCGC 4 cut(s) 479, 1639, 1681, 1777
HpaII CCGG 1 cut(s) 1443
HphI GGTGA 4 cut(s) 170, 227, 1589, 1700
Hpy166II GTNNAC 3 cut(s) 218, 253, 1066
Hpy188I TCNGA 6 cut(s) 328, 396, 817, 862, 1654, 1909
Hpy8I GTNNAC 3 cut(s) 218, 253, 1066
Hpy99I CGWCG 1 cut(s) 1706
HpyCH4III ACNGT 2 cut(s) 755, 1935
HpyF10VI GCNNNNNNNGC 2 cut(s) 642, 1762
HpyF3I CTNAG 6 cut(s) 420, 945, 1152, 1653, 1749, 1853
Hsp92I GRCGYC 1 cut(s) 1639
Hsp92II CATG 9 cut(s) 490, 732, 1072, 1742, 1747, 1760, 1777, 1798, 1882
HspAI GCGC 4 cut(s) 479, 1639, 1681, 1777
KasI GGCGCC 1 cut(s) 1638
Kzo9I GATC 5 cut(s) 59, 986, 1249, 1362, 1514
LguI GCTCTTC 1 cut(s) 1294
LmnI GCTCC 6 cut(s) 707, 896, 964, 1201, 1306, 1328
Lsp1109I GCAGC 4 cut(s) 988, 1010, 1125, 1631
LweI GCATC 3 cut(s) 1573, 1743, 2025
MabI ACCWGGT 1 cut(s) 1524
MaeIII GTNAC 1 cut(s) 1602
MalI GATC 5 cut(s) 61, 988, 1251, 1364, 1516
MboI GATC 5 cut(s) 59, 986, 1249, 1362, 1514
MfeI CAATTG 1 cut(s) 1317
MflI RGATCY 2 cut(s) 59, 1249
MhlI GDGCHC 4 cut(s) 373, 1303, 1998, 2051
MluCI AATT 9 cut(s) 445, 766, 772, 1009, 1237, 1257, 1280, 1317, 1892
MluI ACGCGT 1 cut(s) 1699
Mly113I GGCGCC 1 cut(s) 1639
MlyI GAGTC 2 cut(s) 419, 1273
MmeI TCCRAC 2 cut(s) 93, 990
Mph1103I ATGCAT 2 cut(s) 1047, 1758
MroXI GAANNNNTTC 1 cut(s) 1507
MseI TTAA 6 cut(s) 101, 173, 770, 1157, 1175, 1590
MslI CAYNNNNRTG 2 cut(s) 80, 1050
MspA1I CMGCKG 1 cut(s) 1001
MspCI CTTAAG 1 cut(s) 1174
MspI CCGG 1 cut(s) 1443
MspR9I CCNGG 7 cut(s) 363, 677, 835, 939, 1275, 1443, 1526
MunI CAATTG 1 cut(s) 1317
Mva1269I GAATGC 4 cut(s) 1182, 1204, 1556, 1678
MvaI CCWGG 6 cut(s) 363, 677, 835, 939, 1275, 1526
MvnI CGCG 1 cut(s) 1701
MwoI GCNNNNNNNGC 2 cut(s) 642, 1762
NarI GGCGCC 1 cut(s) 1639
NciI CCSGG 1 cut(s) 1443
NdeI CATATG 1 cut(s) 1047
NdeII GATC 5 cut(s) 59, 986, 1249, 1362, 1514
NlaIII CATG 9 cut(s) 490, 732, 1072, 1742, 1747, 1760, 1777, 1798, 1882
NlaIV GGNNCC 6 cut(s) 1021, 1251, 1272, 1324, 1640, 1932
NmuCI GTSAC 1 cut(s) 1602
NsbI TGCGCA 1 cut(s) 1682
NsiI ATGCAT 2 cut(s) 1047, 1758
NspI RCATGY 3 cut(s) 1072, 1777, 1798
PciI ACATGT 1 cut(s) 1794
PciSI GCTCTTC 1 cut(s) 1294
PctI GAATGC 4 cut(s) 1182, 1204, 1556, 1678
PdmI GAANNNNTTC 1 cut(s) 1507
PflMI CCANNNNNTGG 1 cut(s) 1260
PkrI GCNGC 4 cut(s) 1000, 1003, 1115, 1621
PleI GAGTC 2 cut(s) 419, 1272
PluTI GGCGCC 1 cut(s) 1642
PpsI GAGTC 2 cut(s) 419, 1272
PpuMI RGGWCCY 1 cut(s) 1478
PscI ACATGT 1 cut(s) 1794
PsiI TTATAA 1 cut(s) 1461
Psp124BI GAGCTC 1 cut(s) 373
Psp5II RGGWCCY 1 cut(s) 1478
Psp6I CCWGG 6 cut(s) 361, 675, 833, 937, 1273, 1524
PspFI CCCAGC 1 cut(s) 1918
PspGI CCWGG 6 cut(s) 361, 675, 833, 937, 1273, 1524
PspN4I GGNNCC 6 cut(s) 1021, 1251, 1272, 1324, 1640, 1932
PspPI GGNCC 8 cut(s) 407, 655, 740, 755, 941, 1019, 1270, 1478
PspPPI RGGWCCY 1 cut(s) 1478
PsrI GAACNNNNNNTAC 2 cut(s) 598, 630
PstI CTGCAG 1 cut(s) 1000
PstNI CAGNNNCTG 2 cut(s) 1481, 1606
PsuI RGATCY 2 cut(s) 59, 1249
PvuII CAGCTG 1 cut(s) 1001
RsaI GTAC 2 cut(s) 667, 1470
RsaNI GTAC 2 cut(s) 666, 1469
RseI CAYNNNNRTG 2 cut(s) 80, 1050
SacI GAGCTC 1 cut(s) 373
SapI GCTCTTC 1 cut(s) 1294
SaqAI TTAA 6 cut(s) 101, 173, 770, 1157, 1175, 1590
SatI GCNGC 4 cut(s) 999, 1002, 1114, 1620
Sau3AI GATC 5 cut(s) 59, 986, 1249, 1362, 1514
Sau96I GGNCC 8 cut(s) 407, 655, 740, 755, 941, 1019, 1270, 1478
SchI GAGTC 2 cut(s) 419, 1273
ScrFI CCNGG 7 cut(s) 363, 677, 835, 939, 1275, 1443, 1526
SduI GDGCHC 4 cut(s) 373, 1303, 1998, 2051
SexAI ACCWGGT 1 cut(s) 1524
SfaNI GCATC 3 cut(s) 1573, 1743, 2025
SfcI CTRYAG 2 cut(s) 996, 1293
SfoI GGCGCC 1 cut(s) 1640
SinI GGWCC 4 cut(s) 740, 755, 1019, 1478
SmiI ATTTAAAT 1 cut(s) 771
SmiMI CAYNNNNRTG 2 cut(s) 80, 1050
SmlI CTYRAG 5 cut(s) 202, 226, 659, 826, 1174
SmoI CTYRAG 5 cut(s) 202, 226, 659, 826, 1174
SpeI ACTAGT 2 cut(s) 77, 722
Sse9I AATT 9 cut(s) 445, 766, 772, 1009, 1237, 1257, 1280, 1317, 1892
SsiI CCGC 3 cut(s) 405, 910, 1609
SspDI GGCGCC 1 cut(s) 1638
SstI GAGCTC 1 cut(s) 373
StyD4I CCNGG 7 cut(s) 361, 675, 833, 937, 1273, 1441, 1524
StyI CCWWGG 2 cut(s) 1082, 1547
SwaI ATTTAAAT 1 cut(s) 771
TaaI ACNGT 2 cut(s) 755, 1935
TaqI TCGA 5 cut(s) 1208, 1513, 1629, 1704, 1782
TasI AATT 9 cut(s) 445, 766, 772, 1009, 1237, 1257, 1280, 1317, 1892
Tru1I TTAA 6 cut(s) 101, 173, 770, 1157, 1175, 1590
Tru9I TTAA 6 cut(s) 101, 173, 770, 1157, 1175, 1590
TscAI CASTG 6 cut(s) 255, 544, 583, 912, 1611, 1852
TseFI GTSAC 1 cut(s) 1602
TseI GCWGC 4 cut(s) 998, 1001, 1113, 1619
Tsp45I GTSAC 1 cut(s) 1602
TspDTI ATGAA 7 cut(s) 98, 281, 555, 864, 1720, 1886, 1895
TspGWI ACGGA 3 cut(s) 156, 282, 1355
TspRI CASTG 6 cut(s) 255, 544, 583, 912, 1611, 1852
Van91I CCANNNNNTGG 1 cut(s) 1260
Vha464I CTTAAG 1 cut(s) 1174
VpaK11BI GGWCC 4 cut(s) 740, 755, 1019, 1478
XagI CCTNNNNNAGG 1 cut(s) 876
XapI RAATTY 6 cut(s) 445, 772, 1009, 1237, 1280, 1892
XceI RCATGY 3 cut(s) 1072, 1777, 1798
XcmI CCANNNNNNNNNTGG 4 cut(s) 203, 683, 1105, 1195
XmaJI CCTAGG 1 cut(s) 1547
XmnI GAANNNNTTC 1 cut(s) 1507
Zsp2I ATGCAT 2 cut(s) 1047, 1758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.