pycom05g09590

QWRF motif-containing protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005693
Physical Location & Seq
Forward (+)
12785228 .. 12792066
6839 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1983 bp
ATGATGGTGGCTGCCATTTCCGAAGCAGCTCCGACCGACCCGCCGTCTAACCCGAAAACCTCATCGGGCCACCGCTCACGGCCCCATCTCCAAAACCCATCAAGGCCTCCTCTCTTACCTTCCGAGAAAGACAATGGCTTCGTTCAGAGGCGACCCAGAGGCCGCCAAGTCTCGTCGAAATACATGTCTCCTTCGCCTTCTTCTTCGACCTCGACCTCGACTTCTACGGTCTCGTCCTCTGCGTCTTCCAGAAGATGCCCATCTCCGTTGCTCTCAAGGTCCATAAACTTGGCTTCGAACTCGACCCCTGTTCCGGCTCAGAAACGGGCTCAATCGGCGGACCGGCGGCGACCCATTACTCCTCGGACCTCCGCTGGCCTCCCCGAGGCTCGGGTGAGCAATGCCGGTTCCGAAGTCTCTGCTGCAACTCGGCTTTTGGTCACTTCCACTCGGAGCTTGTCGGTTTCGTTCCAAGGTGAGGCCTTTTCGCTTCCGATTAGCAAGACTAAGACTCCAGCCTCGCCTAGTGGGACTGCCGCGAGGAAGGCCACGCCTGAGAGACGCCGGTCGACCCCTGTGAGAGGAGATCAAGCTGAGAATTCTAAGCCATCTGATCAGCACCGGTGGCCGGCCAGGACTAGACAATTGAGCTTGGGTGGGACTAATAACTTGTCCAGGAGCATGGACTGCAGTTCGGACAACAGGAAGCTCAGTGGGATTGGATCTGGGATTGTGGGTCGAACACTGCAGCAATCCATGATTGATGAGAGCAGAAGAGCGTCTTTCGATGGTAGATTGTGCTTGGATTTGGGGAATGCTGAGCTTCTGAAGGATGCTAGGCAAAACCCAGATGCTAATTCTGCCCATGAATCCTCCGTGCCGTCTGATCTCACTGCTTCTGATACAGACAGTGTTTCTTCTGGTAGCACTTCAGGTGTGCAGGATGCTGGTGGTGCCAAAAAGGGTCGAACCTTACCCCGTGGCATTGCAGTGTCAGCAAGGTTTTGGCAGGAGACTAACAGCCGGTTGAGGCGGTTGCAGGATCCTGGTTCGCCTTTATCAACAACCCCTGTGTCTAGAGCAGGGGCAAAATTCGTTCAATCTCAATCGAAAAGGCTTAATGGTGATGTCCCATTATCATCGTCTCCCCGGACAATGGCTTCCCCCACTAGGGGACCAACTAGGCCTGCTTCGCCGGGTAAGCTTTGGACTTCTTCATCAATGTCACCATCAAGGGGTTATAGTCCTTCTCGGAGAAGTAGTGTTGAAAGTACCTTGAATAGGAGTTATTCTGGTCCTGCCCCTTCAATCCTCAGTTTTTCTGTTGACACTCGGAGAGGGAAGATGGGGGAAGATAGGATTGTCGATGCACACATGCTCAGGCTTCTGTATAACCGTTATCTGCAATGGCGGTTTGTAAATGCAAGATCAGATGCTACCTTCATGGTGCAGAGACGAAATGCCGAGAAAAACATGTGGAATGCATGGGTAACAACTTCAGAGCTCCGACATTCTGTCACACTCAAAAAGATCAAGTTACTCTTGTTGAGGCAAAAATTGAAGTTAACTTCTATCCTCAGAGGACAAATTAGTTGTTTGGAAGAATGGGGTCTTCTGGAAAAAGATCACTCTAGTTCTTTGCTAGGAGCAACTGAAGCTTTGCAGGCCAGTACTCTCCGTCTTCCAGTTGTGGGAAAAGCAATGGTTGACTTTCAAAAGCTGAAAGAAGCTGTGGGTTCGGCAGCTGATGTGATGCAGACAATGGTATCCTCGATATGCTATCTTTCATCAAAGGTGGAGGAAATGAATTCCTTGTTGTGTGAACTTATGAAGGTTACTGCAACAGAACGGGTTGTGCTTGAACAATGCAAAGATTTTTTGTCCACATTATCCGCCATGCAGGTCAAGGATTGTAGCTTGAGAACGCATATAATACAATTGCAACGTGTACCAACTATCCGAAGCCTGACAACACGTGTGTAG

Protein Analysis

661

Amino Acids

71.7

Weight (kDa)

10.81

Isoelectric Point (pI)

65.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 304 - 621 1.2e-111 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1891
AccB1I GGYRCC 1 cut(s) 953
AccBSI CCGCTC 1 cut(s) 75
AccI GTMKAC 1 cut(s) 569
AccII CGCG 1 cut(s) 539
AclWI GGATC 3 cut(s) 730, 1037, 1050
AcoI YGGCCR 2 cut(s) 626, 630
AcsI RAATTY 3 cut(s) 598, 1091, 1807
AcuI CTGAAG 4 cut(s) 848, 915, 1482, 1674
AcvI CACGTG 1 cut(s) 1976
AcyI GRCGYC 1 cut(s) 562
AfaI GTAC 3 cut(s) 1273, 1672, 1950
AflIII ACRYGT 5 cut(s) 183, 1473, 1945, 1973, 1975
AgeI ACCGGT 1 cut(s) 621
AgsI TTSAA 7 cut(s) 1100, 1268, 1279, 1308, 1561, 1715, 1862
AhdI GACNNNNNGTC 2 cut(s) 43, 1514
AjnI CCWGG 3 cut(s) 632, 674, 1045
Alw21I GWGCWC 1 cut(s) 1506
Alw26I GTCTC 8 cut(s) 175, 192, 235, 421, 553, 1007, 1149, 1447
AlwI GGATC 3 cut(s) 730, 1037, 1050
Ama87I CYCGRG 2 cut(s) 383, 390
ApeKI GCWGC 5 cut(s) 11, 26, 422, 748, 1742
ApoI RAATTY 3 cut(s) 598, 1091, 1807
ArsI GACNNNNNNTTYG 4 cut(s) 122, 154, 170, 202
AsiGI ACCGGT 1 cut(s) 621
AspS9I GGNCC 7 cut(s) 67, 81, 279, 340, 366, 1175, 1295
AsuC2I CCSGG 2 cut(s) 1150, 1197
AsuHPI GGTGA 4 cut(s) 406, 488, 1136, 1218
AsuII TTCGAA 1 cut(s) 296
AvaI CYCGRG 2 cut(s) 383, 390
AvaII GGWCC 5 cut(s) 279, 340, 366, 1175, 1295
BaeI ACNNNNGTAYC 2 cut(s) 1749, 1782
BamHI GGATCC 1 cut(s) 1042
BanI GGYRCC 1 cut(s) 953
BanII GRGCYC 2 cut(s) 331, 1506
BbrPI CACGTG 1 cut(s) 1976
BbsI GAAGAC 3 cut(s) 237, 1604, 1673
Bbv12I GWGCWC 1 cut(s) 1506
BbvI GCAGC 4 cut(s) 38, 409, 760, 1754
BccI CCATC 7 cut(s) 93, 106, 268, 616, 782, 1237, 1339
BceAI ACGGC 3 cut(s) 28, 95, 865
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 3 cut(s) 634, 676, 1047
BciVI GTATCC 1 cut(s) 1777
BclI TGATCA 1 cut(s) 613
BcnI CCSGG 2 cut(s) 1150, 1197
BcoDI GTCTC 8 cut(s) 175, 192, 235, 421, 553, 1007, 1149, 1447
BfaI CTAG 8 cut(s) 525, 639, 837, 1077, 1170, 1182, 1632, 1643
BfmI CTRYAG 2 cut(s) 688, 746
BfuAI ACCTGC 1 cut(s) 1891
BfuI GTATCC 1 cut(s) 1777
BisI GCNGC 8 cut(s) 12, 27, 163, 347, 423, 537, 749, 1743
BlpI GCTNAGC 1 cut(s) 819
BlsI GCNGC 8 cut(s) 13, 28, 164, 348, 424, 538, 750, 1744
BmcAI AGTACT 1 cut(s) 1672
Bme1390I CCNGG 5 cut(s) 634, 676, 1047, 1150, 1197
Bme18I GGWCC 5 cut(s) 279, 340, 366, 1175, 1295
BmeRI GACNNNNNGTC 2 cut(s) 43, 1514
BmeT110I CYCGRG 2 cut(s) 383, 390
BmgT120I GGNCC 7 cut(s) 67, 81, 279, 340, 366, 1175, 1295
BmiI GGNNCC 5 cut(s) 83, 409, 955, 1044, 1176
BmrFI CCNGG 5 cut(s) 634, 676, 1047, 1150, 1197
BmsI GCATC 7 cut(s) 245, 823, 841, 934, 1357, 1423, 1743
BoxI GACNNNNGTC 1 cut(s) 565
BpiI GAAGAC 3 cut(s) 237, 1604, 1673
BpmI CTGGAG 1 cut(s) 498
Bpu10I CCTNAGC 1 cut(s) 1379
Bpu1102I GCTNAGC 1 cut(s) 819
Bpu14I TTCGAA 1 cut(s) 296
BpuEI CTTGAG 2 cut(s) 259, 1939
BpuMI CCSGG 2 cut(s) 1150, 1197
BsaAI YACGTR 1 cut(s) 1976
BsaBI GATNNNNATC 1 cut(s) 259
BsaHI GRCGYC 1 cut(s) 562
BsaI GGTCTC 1 cut(s) 235
BsaJI CCNNGG 5 cut(s) 362, 384, 472, 979, 1148
BsaWI WCCGGW 1 cut(s) 621
BsaXI ACNNNNNCTCC 4 cut(s) 496, 526, 1246, 1276
Bse118I RCCGGY 6 cut(s) 342, 404, 564, 621, 628, 1023
Bse1I ACTGG 2 cut(s) 1668, 1685
Bse3DI GCAATG 4 cut(s) 406, 984, 1412, 1707
Bse8I GATNNNNATC 1 cut(s) 259
BseBI CCWGG 3 cut(s) 634, 676, 1047
BseDI CCNNGG 5 cut(s) 362, 384, 472, 979, 1148
BseGI GGATG 2 cut(s) 838, 949
BseJI GATNNNNATC 1 cut(s) 259
BseMI GCAATG 4 cut(s) 406, 984, 1412, 1707
BseMII CTCAG 8 cut(s) 332, 546, 585, 724, 810, 1327, 1393, 1591
BseNI ACTGG 2 cut(s) 1668, 1685
BseRI GAGGAG 3 cut(s) 99, 351, 597
BseXI GCAGC 4 cut(s) 38, 409, 760, 1754
BsgI GTGCAG 2 cut(s) 959, 1469
Bsh1236I CGCG 1 cut(s) 539
Bsh1285I CGRYCG 2 cut(s) 36, 569
BshNI GGYRCC 1 cut(s) 953
BshTI ACCGGT 1 cut(s) 621
BsiEI CGRYCG 2 cut(s) 36, 569
BsiHKAI GWGCWC 1 cut(s) 1506
BsiHKCI CYCGRG 2 cut(s) 383, 390
BsiSI CCGG 9 cut(s) 314, 343, 405, 565, 622, 629, 1024, 1150, 1196
BslFI GGGAC 4 cut(s) 544, 673, 1115, 1188
BsmAI GTCTC 8 cut(s) 175, 192, 235, 421, 553, 1007, 1149, 1447
BsmBI CGTCTC 3 cut(s) 553, 1149, 1447
BsmFI GGGAC 4 cut(s) 544, 673, 1115, 1188
BsmI GAATGC 2 cut(s) 820, 1486
Bso31I GGTCTC 1 cut(s) 235
BsoBI CYCGRG 2 cut(s) 383, 390
Bsp119I TTCGAA 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 331, 1506
Bsp143I GATC 8 cut(s) 586, 613, 722, 886, 1042, 1427, 1530, 1624
Bsp1720I GCTNAGC 1 cut(s) 819
BspCNI CTCAG 8 cut(s) 331, 547, 586, 723, 811, 1326, 1392, 1590
BspFNI CGCG 1 cut(s) 539
BspLI GGNNCC 5 cut(s) 83, 409, 955, 1044, 1176
BspMAI CTGCAG 2 cut(s) 692, 750
BspMI ACCTGC 1 cut(s) 1891
BspPI GGATC 3 cut(s) 730, 1037, 1050
BspQI GCTCTTC 1 cut(s) 769
BspT104I TTCGAA 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 953
BspTNI GGTCTC 1 cut(s) 235
BsrBI CCGCTC 1 cut(s) 75
BsrDI GCAATG 4 cut(s) 406, 984, 1412, 1707
BsrFI RCCGGY 6 cut(s) 342, 404, 564, 621, 628, 1023
BsrI ACTGG 2 cut(s) 1668, 1685
BssAI RCCGGY 6 cut(s) 342, 404, 564, 621, 628, 1023
BssECI CCNNGG 5 cut(s) 362, 384, 472, 979, 1148
BssMI GATC 8 cut(s) 586, 613, 722, 886, 1042, 1427, 1530, 1624
BssNI GRCGYC 1 cut(s) 562
BssT1I CCWWGG 1 cut(s) 472
Bst2UI CCWGG 3 cut(s) 634, 676, 1047
Bst4CI ACNGT 3 cut(s) 229, 911, 1397
Bst6I CTCTTC 1 cut(s) 769
BstACI GRCGYC 1 cut(s) 562
BstAPI GCANNNNNTGC 1 cut(s) 687
BstBAI YACGTR 1 cut(s) 1976
BstBI TTCGAA 1 cut(s) 296
BstC8I GCNNGC 4 cut(s) 376, 630, 1188, 1665
BstDSI CCRYGG 1 cut(s) 979
BstENI CCTNNNNNAGG 3 cut(s) 383, 579, 1279
BstF5I GGATG 2 cut(s) 838, 949
BstFNI CGCG 1 cut(s) 539
BstKTI GATC 8 cut(s) 589, 616, 725, 889, 1045, 1430, 1533, 1627
BstMAI GTCTC 8 cut(s) 175, 192, 235, 421, 553, 1007, 1149, 1447
BstMBI GATC 8 cut(s) 586, 613, 722, 886, 1042, 1427, 1530, 1624
BstMCI CGRYCG 2 cut(s) 36, 569
BstNI CCWGG 3 cut(s) 634, 676, 1047
BstNSI RCATGY 3 cut(s) 187, 1378, 1477
BstPAI GACNNNNGTC 1 cut(s) 565
BstSCI CCNGG 5 cut(s) 632, 674, 1045, 1148, 1195
BstSFI CTRYAG 2 cut(s) 688, 746
BstUI CGCG 1 cut(s) 539
BstV1I GCAGC 4 cut(s) 38, 409, 760, 1754
BstV2I GAAGAC 3 cut(s) 237, 1604, 1673
BstX2I RGATCY 2 cut(s) 722, 1042
BstXI CCANNNNNNTGG 2 cut(s) 289, 682
BstYI RGATCY 2 cut(s) 722, 1042
BsuI GTATCC 1 cut(s) 1777
BtgI CCRYGG 1 cut(s) 979
BtsCI GGATG 2 cut(s) 838, 949
BtsI GCAGTG 3 cut(s) 743, 891, 996
BtsIMutI CAGTG 5 cut(s) 718, 743, 891, 916, 996
BveI ACCTGC 1 cut(s) 1891
Cac8I GCNNGC 4 cut(s) 376, 630, 1188, 1665
Cfr10I RCCGGY 6 cut(s) 342, 404, 564, 621, 628, 1023
Cfr13I GGNCC 7 cut(s) 67, 81, 279, 340, 366, 1175, 1295
CpoI CGGWCCG 1 cut(s) 340
CseI GACGC 3 cut(s) 231, 570, 768
Csp6I GTAC 3 cut(s) 1272, 1671, 1949
CspAI ACCGGT 1 cut(s) 621
CspI CGGWCCG 1 cut(s) 340
CviAII CATG 9 cut(s) 184, 682, 757, 866, 1375, 1444, 1474, 1485, 1897
CviQI GTAC 3 cut(s) 1272, 1671, 1949
DpnI GATC 8 cut(s) 588, 615, 724, 888, 1044, 1429, 1532, 1626
DpnII GATC 8 cut(s) 586, 613, 722, 886, 1042, 1427, 1530, 1624
DriI GACNNNNNGTC 2 cut(s) 43, 1514
EaeI YGGCCR 2 cut(s) 626, 630
Eam1104I CTCTTC 1 cut(s) 769
Eam1105I GACNNNNNGTC 2 cut(s) 43, 1514
EarI CTCTTC 1 cut(s) 769
EciI GGCGGA 2 cut(s) 353, 1882
Ecl136II GAGCTC 1 cut(s) 1504
Eco130I CCWWGG 1 cut(s) 472
Eco147I AGGCCT 3 cut(s) 106, 482, 1186
Eco24I GRGCYC 2 cut(s) 331, 1506
Eco31I GGTCTC 1 cut(s) 235
Eco47I GGWCC 5 cut(s) 279, 340, 366, 1175, 1295
Eco53kI GAGCTC 1 cut(s) 1504
Eco57I CTGAAG 4 cut(s) 848, 915, 1482, 1674
Eco72I CACGTG 1 cut(s) 1976
Eco88I CYCGRG 2 cut(s) 383, 390
EcoICRI GAGCTC 1 cut(s) 1504
EcoNI CCTNNNNNAGG 3 cut(s) 383, 579, 1279
EcoRI GAATTC 2 cut(s) 598, 1807
EcoRII CCWGG 3 cut(s) 632, 674, 1045
EcoT14I CCWWGG 1 cut(s) 472
EcoT22I ATGCAT 1 cut(s) 1486
EcoT38I GRGCYC 2 cut(s) 331, 1506
ErhI CCWWGG 1 cut(s) 472
Esp3I CGTCTC 3 cut(s) 553, 1149, 1447
FaeI CATG 9 cut(s) 187, 685, 760, 869, 1378, 1447, 1477, 1488, 1900
FalI AAGNNNNNCTT 4 cut(s) 766, 798, 1526, 1558
FaqI GGGAC 4 cut(s) 544, 673, 1115, 1188
FatI CATG 9 cut(s) 183, 681, 756, 865, 1374, 1443, 1473, 1484, 1896
FauI CCCGC 1 cut(s) 48
FbaI TGATCA 1 cut(s) 613
FblI GTMKAC 1 cut(s) 569
Fnu4HI GCNGC 8 cut(s) 12, 27, 163, 347, 423, 537, 749, 1743
FokI GGATG 2 cut(s) 845, 956
FriOI GRGCYC 2 cut(s) 331, 1506
FseI GGCCGGCC 1 cut(s) 632
Fsp4HI GCNGC 8 cut(s) 12, 27, 163, 347, 423, 537, 749, 1743
FspBI CTAG 8 cut(s) 525, 639, 837, 1077, 1170, 1182, 1632, 1643
GluI GCNGC 8 cut(s) 12, 27, 163, 347, 423, 537, 749, 1743
GsuI CTGGAG 1 cut(s) 498
HapII CCGG 9 cut(s) 314, 343, 405, 565, 622, 629, 1024, 1150, 1196
HgaI GACGC 3 cut(s) 231, 570, 768
Hin1I GRCGYC 1 cut(s) 562
Hin1II CATG 9 cut(s) 187, 685, 760, 869, 1378, 1447, 1477, 1488, 1900
HincII GTYRAC 4 cut(s) 570, 1327, 1566, 1708
HindII GTYRAC 4 cut(s) 570, 1327, 1566, 1708
HindIII AAGCTT 2 cut(s) 1202, 1656
HinfI GANTC 2 cut(s) 511, 869
HpaI GTTAAC 1 cut(s) 1566
HpaII CCGG 9 cut(s) 314, 343, 405, 565, 622, 629, 1024, 1150, 1196
HphI GGTGA 4 cut(s) 406, 488, 1136, 1218
Hpy166II GTNNAC 7 cut(s) 570, 1327, 1566, 1708, 1823, 1884, 1949
Hpy188III TCNNGA 3 cut(s) 249, 1077, 1616
Hpy8I GTNNAC 7 cut(s) 570, 1327, 1566, 1708, 1823, 1884, 1949
Hpy99I CGWCG 1 cut(s) 178
HpyAV CCTTC 9 cut(s) 129, 201, 207, 538, 823, 1257, 1314, 1450, 1825
HpyCH4III ACNGT 3 cut(s) 229, 911, 1397
HpyCH4IV ACGT 2 cut(s) 1945, 1975
HpySE526I ACGT 2 cut(s) 1945, 1975
Hsp92I GRCGYC 1 cut(s) 562
Hsp92II CATG 9 cut(s) 187, 685, 760, 869, 1378, 1447, 1477, 1488, 1900
KroI GCCGGC 1 cut(s) 628
KroNI GCCGGC 1 cut(s) 630
Ksp22I TGATCA 1 cut(s) 613
KspAI GTTAAC 1 cut(s) 1566
Kzo9I GATC 8 cut(s) 586, 613, 722, 886, 1042, 1427, 1530, 1624
LguI GCTCTTC 1 cut(s) 769
LmnI GCTCC 5 cut(s) 34, 453, 678, 1509, 1646
Lsp1109I GCAGC 4 cut(s) 38, 409, 760, 1754
LweI GCATC 7 cut(s) 245, 823, 841, 934, 1357, 1423, 1743
MaeI CTAG 8 cut(s) 525, 639, 837, 1077, 1170, 1182, 1632, 1643
MaeII ACGT 2 cut(s) 1945, 1975
MaeIII GTNAC 6 cut(s) 439, 1224, 1489, 1516, 1536, 1834
MalI GATC 8 cut(s) 588, 615, 724, 888, 1044, 1429, 1532, 1626
MbiI CCGCTC 1 cut(s) 75
MboI GATC 8 cut(s) 586, 613, 722, 886, 1042, 1427, 1530, 1624
MfeI CAATTG 2 cut(s) 644, 1937
MflI RGATCY 2 cut(s) 722, 1042
MhlI GDGCHC 2 cut(s) 331, 1506
MluCI AATT 8 cut(s) 598, 644, 856, 1091, 1556, 1587, 1807, 1937
MlyI GAGTC 1 cut(s) 505
MmeI TCCRAC 2 cut(s) 56, 1531
Mph1103I ATGCAT 1 cut(s) 1486
MroNI GCCGGC 1 cut(s) 628
MseI TTAA 2 cut(s) 1119, 1565
MslI CAYNNNNRTG 1 cut(s) 989
MspA1I CMGCKG 2 cut(s) 374, 1745
MspI CCGG 9 cut(s) 314, 343, 405, 565, 622, 629, 1024, 1150, 1196
MspR9I CCNGG 5 cut(s) 634, 676, 1047, 1150, 1197
MunI CAATTG 2 cut(s) 644, 1937
Mva1269I GAATGC 2 cut(s) 820, 1486
MvaI CCWGG 3 cut(s) 634, 676, 1047
MvnI CGCG 1 cut(s) 539
NaeI GCCGGC 1 cut(s) 630
NciI CCSGG 2 cut(s) 1150, 1197
NdeII GATC 8 cut(s) 586, 613, 722, 886, 1042, 1427, 1530, 1624
NgoMIV GCCGGC 1 cut(s) 628
NlaIII CATG 9 cut(s) 187, 685, 760, 869, 1378, 1447, 1477, 1488, 1900
NlaIV GGNNCC 5 cut(s) 83, 409, 955, 1044, 1176
NmeAIII GCCGAG 2 cut(s) 409, 1489
NmuCI GTSAC 3 cut(s) 439, 1224, 1516
NsiI ATGCAT 1 cut(s) 1486
NspI RCATGY 3 cut(s) 187, 1378, 1477
NspV TTCGAA 1 cut(s) 296
PceI AGGCCT 3 cut(s) 106, 482, 1186
PciI ACATGT 2 cut(s) 183, 1473
PciSI GCTCTTC 1 cut(s) 769
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PctI GAATGC 2 cut(s) 820, 1486
PdiI GCCGGC 1 cut(s) 630
PfeI GAWTC 1 cut(s) 869
PfoI TCCNGGA 1 cut(s) 674
PinAI ACCGGT 1 cut(s) 621
PkrI GCNGC 8 cut(s) 13, 28, 164, 348, 424, 538, 750, 1744
PleI GAGTC 1 cut(s) 505
PmaCI CACGTG 1 cut(s) 1976
PmlI CACGTG 1 cut(s) 1976
PpsI GAGTC 1 cut(s) 505
Ppu21I YACGTR 1 cut(s) 1976
PscI ACATGT 2 cut(s) 183, 1473
PshAI GACNNNNGTC 1 cut(s) 565
Psp124BI GAGCTC 1 cut(s) 1506
Psp6I CCWGG 3 cut(s) 632, 674, 1045
PspCI CACGTG 1 cut(s) 1976
PspGI CCWGG 3 cut(s) 632, 674, 1045
PspN4I GGNNCC 5 cut(s) 83, 409, 955, 1044, 1176
PspPI GGNCC 7 cut(s) 67, 81, 279, 340, 366, 1175, 1295
PstI CTGCAG 2 cut(s) 692, 750
PsuI RGATCY 2 cut(s) 722, 1042
PvuII CAGCTG 1 cut(s) 1745
RigI GGCCGGCC 1 cut(s) 632
RsaI GTAC 3 cut(s) 1273, 1672, 1950
RsaNI GTAC 3 cut(s) 1272, 1671, 1949
RseI CAYNNNNRTG 1 cut(s) 989
Rsr2I CGGWCCG 1 cut(s) 340
RsrII CGGWCCG 1 cut(s) 340
SacI GAGCTC 1 cut(s) 1506
SalI GTCGAC 1 cut(s) 568
SapI GCTCTTC 1 cut(s) 769
SaqAI TTAA 2 cut(s) 1119, 1565
SatI GCNGC 8 cut(s) 12, 27, 163, 347, 423, 537, 749, 1743
Sau3AI GATC 8 cut(s) 586, 613, 722, 886, 1042, 1427, 1530, 1624
Sau96I GGNCC 7 cut(s) 67, 81, 279, 340, 366, 1175, 1295
ScaI AGTACT 1 cut(s) 1672
SchI GAGTC 1 cut(s) 505
ScrFI CCNGG 5 cut(s) 634, 676, 1047, 1150, 1197
SduI GDGCHC 2 cut(s) 331, 1506
SfaNI GCATC 7 cut(s) 245, 823, 841, 934, 1357, 1423, 1743
SfcI CTRYAG 2 cut(s) 688, 746
SfuI TTCGAA 1 cut(s) 296
SgrAI CRCCGGYG 1 cut(s) 621
SinI GGWCC 5 cut(s) 279, 340, 366, 1175, 1295
SmiMI CAYNNNNRTG 1 cut(s) 989
SmlI CTYRAG 2 cut(s) 274, 1918
SmoI CTYRAG 2 cut(s) 274, 1918
Sse9I AATT 8 cut(s) 598, 644, 856, 1091, 1556, 1587, 1807, 1937
SseBI AGGCCT 3 cut(s) 106, 482, 1186
SspMI CTAG 8 cut(s) 525, 639, 837, 1077, 1170, 1182, 1632, 1643
SstI GAGCTC 1 cut(s) 1506
StuI AGGCCT 3 cut(s) 106, 482, 1186
StyD4I CCNGG 5 cut(s) 632, 674, 1045, 1148, 1195
StyI CCWWGG 1 cut(s) 472
TaaI ACNGT 3 cut(s) 229, 911, 1397
TaiI ACGT 2 cut(s) 1948, 1978
TaqII GACCGA 1 cut(s) 50
TasI AATT 8 cut(s) 598, 644, 856, 1091, 1556, 1587, 1807, 1937
TatI WGTACW 1 cut(s) 1670
TauI GCSGC 3 cut(s) 165, 349, 539
TfiI GAWTC 1 cut(s) 869
Tru1I TTAA 2 cut(s) 1119, 1565
Tru9I TTAA 2 cut(s) 1119, 1565
TscAI CASTG 5 cut(s) 718, 750, 898, 916, 996
TseFI GTSAC 3 cut(s) 439, 1224, 1516
TseI GCWGC 5 cut(s) 11, 26, 422, 748, 1742
Tsp45I GTSAC 3 cut(s) 439, 1224, 1516
TspDTI ATGAA 6 cut(s) 882, 1206, 1432, 1776, 1820, 1844
TspGWI ACGGA 3 cut(s) 255, 865, 1667
TspRI CASTG 5 cut(s) 718, 750, 898, 916, 996
VpaK11BI GGWCC 5 cut(s) 279, 340, 366, 1175, 1295
XagI CCTNNNNNAGG 3 cut(s) 383, 579, 1279
XapI RAATTY 3 cut(s) 598, 1091, 1807
XbaI TCTAGA 1 cut(s) 1076
XceI RCATGY 3 cut(s) 187, 1378, 1477
XmiI GTMKAC 1 cut(s) 569
XspI CTAG 8 cut(s) 525, 639, 837, 1077, 1170, 1182, 1632, 1643
ZrmI AGTACT 1 cut(s) 1672
Zsp2I ATGCAT 1 cut(s) 1486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.