pycom05g21740

Divergent PAP2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006452
Physical Location & Seq
Forward (+)
24301591 .. 24303835
2245 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 975 bp
ATGGTTTTTTTTTTGTTAAAATTGAACAGTAGCAGAAGTACATCAACGACGTCGTTCGAGGTTTTCCATTTTTTTGGTTGTTATTCACGTGGACAGGGAGACGGCGAGGTCGAGAGACGGCGAGGTCGAGAGACGCCGAGAAAGTCGGAGACAATGTTGCTGCTGCAGCCTTGGAGCGTTTCGAGCTCCACAGCGCTTCACAATTCTCTGGCTTTTTCCCACTCGGAAACCCAGCACAAAGCCATTTATAAACGACGCCGTTCAGCAGCACCCTTTTGCCTCAGAGCTCAGTTGCTGGACGGAATCGATCAGCTCGCGCATAACAAGGTTTTGATTGCGGCTGGAATTTCAATGGCAGTTGGGCAGCTCACGAAGCCGGTGACTTCTGTGATTTTGTATGGGAAAGAGTTTGATTTCAAGGCGGTCGTTCAGGCTGGGGGATTCCCTTCTTCACATTCCTCTGCAATGGTGGCTACTGCTACAACCCTTGGCCTTGAAAAGGGCCTTTCGGATTCAATATTTGGCCTTACCGTTGTTTATGCAGGCATTGTGATGTATGATGCTCAGGGGGTGAGAAGAGAAGTTGGCAATCATGCAAAAGTACTGAACAAAACGCTGCCCCTGAACACAACGGTGAACTCAGTCCCCACCCAGGACGGAGGTAGAATTAACTCTCAACCTAAAAAACCATTGTTGAAGTCGGAAAGCGTTGAGTCTCTGTTGTCTGAGGAAGCAAACATTTTCTCACCAAAAGCAACAAATGGCCCTTTAGCGTCTCAGTCCAAGGAGATAACAATGCAAGCTACTCAGACGCAGGTTTCTTCGGGCTTAGGAAGTAATGCCGAGGAAGGTTTAGAAAGAGCTGCCAATACTTCAACTCGGCTAAAAGAATCGATCGGCCACACTGAAGTAGAAGTCGCGGCCGGCGCTTTGTTGGGTTTCTTGGTAGGATTGGCGGTGTATGCGATAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF212

Protein Analysis

325

Amino Acids

34.77

Weight (kDa)

9.28

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF212 PF02681 107 - 316 2e-36 Divergent PAP2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 249
AasI GACNNNNNNGTC 2 cut(s) 107, 123
AatII GACGTC 1 cut(s) 53
Acc36I ACCTGC 1 cut(s) 805
AccII CGCG 2 cut(s) 317, 920
AciI CCGC 4 cut(s) 338, 422, 920, 956
AcoI YGGCCR 2 cut(s) 898, 921
AcsI RAATTY 1 cut(s) 345
AcuI CTGAAG 1 cut(s) 927
AcvI CACGTG 1 cut(s) 89
AcyI GRCGYC 3 cut(s) 50, 134, 256
AfaI GTAC 2 cut(s) 40, 603
AfeI AGCGCT 1 cut(s) 195
AfiI CCNNNNNNNGG 2 cut(s) 499, 652
AgsI TTSAA 7 cut(s) 25, 351, 418, 497, 516, 697, 876
AjnI CCWGG 1 cut(s) 651
AleI CACNNNNGTG 1 cut(s) 632
AluBI AGCT 6 cut(s) 186, 287, 313, 367, 803, 863
AluI AGCT 6 cut(s) 186, 287, 313, 367, 803, 863
Alw21I GWGCWC 2 cut(s) 188, 289
Alw26I GTCTC 6 cut(s) 93, 109, 125, 143, 720, 780
AlwNI CAGNNNCTG 1 cut(s) 295
Aor51HI AGCGCT 1 cut(s) 195
AoxI GGCC 6 cut(s) 490, 502, 523, 763, 898, 921
ApeKI GCWGC 7 cut(s) 160, 163, 166, 266, 364, 616, 863
ApoI RAATTY 1 cut(s) 345
AspLEI GCGC 3 cut(s) 196, 319, 929
AspS9I GGNCC 2 cut(s) 502, 764
AsuHPI GGTGA 4 cut(s) 391, 583, 646, 738
BanII GRGCYC 2 cut(s) 188, 289
BbrPI CACGTG 1 cut(s) 89
Bbv12I GWGCWC 2 cut(s) 188, 289
BbvI GCAGC 7 cut(s) 147, 150, 178, 278, 376, 603, 850
BceAI ACGGC 3 cut(s) 118, 134, 243
BciT130I CCWGG 1 cut(s) 653
BcoDI GTCTC 6 cut(s) 93, 109, 125, 143, 720, 780
BfmI CTRYAG 1 cut(s) 164
BfoI RGCGCY 2 cut(s) 197, 930
BfuAI ACCTGC 1 cut(s) 805
BisI GCNGC 9 cut(s) 161, 164, 167, 267, 339, 365, 617, 864, 921
BlsI GCNGC 9 cut(s) 162, 165, 168, 268, 340, 366, 618, 865, 922
BmcAI AGTACT 1 cut(s) 603
Bme1390I CCNGG 1 cut(s) 653
BmgT120I GGNCC 2 cut(s) 502, 764
BmrFI CCNGG 1 cut(s) 653
BmsI GCATC 1 cut(s) 550
Bpu10I CCTNAGC 2 cut(s) 564, 829
Bsa29I ATCGAT 2 cut(s) 306, 893
BsaAI YACGTR 1 cut(s) 89
BsaHI GRCGYC 3 cut(s) 50, 134, 256
BsaJI CCNNGG 5 cut(s) 170, 487, 651, 783, 843
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 2 cut(s) 499, 652
Bse118I RCCGGY 2 cut(s) 376, 923
Bse3DI GCAATG 1 cut(s) 471
BseBI CCWGG 1 cut(s) 653
BseCI ATCGAT 2 cut(s) 306, 893
BseDI CCNNGG 5 cut(s) 170, 487, 651, 783, 843
BseLI CCNNNNNNNGG 2 cut(s) 499, 652
BseMI GCAATG 1 cut(s) 471
BseMII CTCAG 7 cut(s) 295, 302, 578, 654, 717, 791, 821
BseX3I CGGCCG 1 cut(s) 921
BseXI GCAGC 7 cut(s) 147, 150, 178, 278, 376, 603, 850
BseYI CCCAGC 2 cut(s) 231, 434
Bsh1236I CGCG 2 cut(s) 317, 920
Bsh1285I CGRYCG 3 cut(s) 426, 897, 924
BshFI GGCC 6 cut(s) 492, 504, 525, 765, 900, 923
BshVI ATCGAT 2 cut(s) 306, 893
BsiEI CGRYCG 3 cut(s) 426, 897, 924
BsiHKAI GWGCWC 2 cut(s) 188, 289
BsiSI CCGG 2 cut(s) 377, 924
BslFI GGGAC 1 cut(s) 629
BslI CCNNNNNNNGG 2 cut(s) 499, 652
BsmAI GTCTC 6 cut(s) 93, 109, 125, 143, 720, 780
BsmBI CGTCTC 4 cut(s) 93, 109, 125, 780
BsmFI GGGAC 1 cut(s) 629
BsnI GGCC 6 cut(s) 492, 504, 525, 765, 900, 923
Bsp1286I GDGCHC 2 cut(s) 188, 289
Bsp143I GATC 2 cut(s) 307, 894
BspACI CCGC 4 cut(s) 338, 422, 920, 956
BspANI GGCC 6 cut(s) 492, 504, 525, 765, 900, 923
BspCNI CTCAG 7 cut(s) 294, 301, 577, 653, 718, 790, 820
BspDI ATCGAT 2 cut(s) 306, 893
BspFNI CGCG 2 cut(s) 317, 920
BspMAI CTGCAG 1 cut(s) 168
BspMI ACCTGC 1 cut(s) 805
BsrDI GCAATG 1 cut(s) 471
BsrFI RCCGGY 2 cut(s) 376, 923
BssAI RCCGGY 2 cut(s) 376, 923
BssECI CCNNGG 5 cut(s) 170, 487, 651, 783, 843
BssMI GATC 2 cut(s) 307, 894
BssNI GRCGYC 3 cut(s) 50, 134, 256
BssT1I CCWWGG 3 cut(s) 170, 487, 783
Bst2UI CCWGG 1 cut(s) 653
Bst4CI ACNGT 3 cut(s) 29, 532, 634
Bst6I CTCTTC 1 cut(s) 571
BstACI GRCGYC 3 cut(s) 50, 134, 256
BstBAI YACGTR 1 cut(s) 89
BstC8I GCNNGC 4 cut(s) 315, 544, 801, 925
BstDEI CTNAG 8 cut(s) 281, 288, 564, 640, 726, 777, 807, 829
BstENI CCTNNNNNAGG 1 cut(s) 497
BstFNI CGCG 2 cut(s) 317, 920
BstH2I RGCGCY 2 cut(s) 197, 930
BstHHI GCGC 3 cut(s) 196, 319, 929
BstKTI GATC 2 cut(s) 310, 897
BstMAI GTCTC 6 cut(s) 93, 109, 125, 143, 720, 780
BstMBI GATC 2 cut(s) 307, 894
BstMCI CGRYCG 3 cut(s) 426, 897, 924
BstMWI GCNNNNNNNGC 6 cut(s) 166, 183, 373, 470, 926, 962
BstNI CCWGG 1 cut(s) 653
BstSCI CCNGG 1 cut(s) 651
BstSFI CTRYAG 1 cut(s) 164
BstUI CGCG 2 cut(s) 317, 920
BstV1I GCAGC 7 cut(s) 147, 150, 178, 278, 376, 603, 850
BstXI CCANNNNNNTGG 1 cut(s) 74
BstZI CGGCCG 1 cut(s) 921
Bsu15I ATCGAT 2 cut(s) 306, 893
BsuRI GGCC 6 cut(s) 492, 504, 525, 765, 900, 923
BsuTUI ATCGAT 2 cut(s) 306, 893
BtsIMutI CAGTG 1 cut(s) 903
BveI ACCTGC 1 cut(s) 805
Cac8I GCNNGC 4 cut(s) 315, 544, 801, 925
CaiI CAGNNNCTG 1 cut(s) 295
CfoI GCGC 3 cut(s) 196, 319, 929
Cfr10I RCCGGY 2 cut(s) 376, 923
Cfr13I GGNCC 2 cut(s) 502, 764
ClaI ATCGAT 2 cut(s) 306, 893
CseI GACGC 4 cut(s) 142, 264, 762, 820
Csp6I GTAC 2 cut(s) 39, 602
CviAII CATG 1 cut(s) 593
CviQI GTAC 2 cut(s) 39, 602
DdeI CTNAG 8 cut(s) 281, 288, 564, 640, 726, 777, 807, 829
DpnI GATC 2 cut(s) 309, 896
DpnII GATC 2 cut(s) 307, 894
DrdI GACNNNNNNGTC 2 cut(s) 107, 123
DseDI GACNNNNNNGTC 2 cut(s) 107, 123
EaeI YGGCCR 2 cut(s) 898, 921
EagI CGGCCG 1 cut(s) 921
Eam1104I CTCTTC 1 cut(s) 571
EarI CTCTTC 1 cut(s) 571
Ecl136II GAGCTC 2 cut(s) 186, 287
EclXI CGGCCG 1 cut(s) 921
Eco130I CCWWGG 3 cut(s) 170, 487, 783
Eco24I GRGCYC 2 cut(s) 188, 289
Eco47III AGCGCT 1 cut(s) 195
Eco52I CGGCCG 1 cut(s) 921
Eco53kI GAGCTC 2 cut(s) 186, 287
Eco57I CTGAAG 1 cut(s) 927
Eco72I CACGTG 1 cut(s) 89
EcoICRI GAGCTC 2 cut(s) 186, 287
EcoNI CCTNNNNNAGG 1 cut(s) 497
EcoO109I RGGNCCY 1 cut(s) 502
EcoRII CCWGG 1 cut(s) 651
EcoT14I CCWWGG 3 cut(s) 170, 487, 783
EcoT38I GRGCYC 2 cut(s) 188, 289
ErhI CCWWGG 3 cut(s) 170, 487, 783
Esp3I CGTCTC 4 cut(s) 93, 109, 125, 780
FaeI CATG 1 cut(s) 596
FaiI YATR 7 cut(s) 249, 321, 399, 540, 558, 594, 963
FaqI GGGAC 1 cut(s) 629
FatI CATG 1 cut(s) 592
Fnu4HI GCNGC 9 cut(s) 161, 164, 167, 267, 339, 365, 617, 864, 921
FriOI GRGCYC 2 cut(s) 188, 289
Fsp4HI GCNGC 9 cut(s) 161, 164, 167, 267, 339, 365, 617, 864, 921
GlaI GCGC 3 cut(s) 195, 318, 928
GluI GCNGC 9 cut(s) 161, 164, 167, 267, 339, 365, 617, 864, 921
GsaI CCCAGC 2 cut(s) 235, 438
HaeII RGCGCY 2 cut(s) 197, 930
HaeIII GGCC 6 cut(s) 492, 504, 525, 765, 900, 923
HapII CCGG 2 cut(s) 377, 924
HgaI GACGC 4 cut(s) 142, 264, 762, 820
HhaI GCGC 3 cut(s) 196, 319, 929
Hin1I GRCGYC 3 cut(s) 50, 134, 256
Hin1II CATG 1 cut(s) 596
Hin6I GCGC 3 cut(s) 194, 317, 927
HinP1I GCGC 3 cut(s) 194, 317, 927
HinfI GANTC 5 cut(s) 303, 441, 512, 713, 890
HpaII CCGG 2 cut(s) 377, 924
HphI GGTGA 4 cut(s) 391, 583, 646, 738
Hpy166II GTNNAC 2 cut(s) 92, 637
Hpy188I TCNGA 7 cut(s) 148, 226, 284, 511, 703, 727, 810
Hpy188III TCNNGA 3 cut(s) 112, 128, 370
Hpy8I GTNNAC 2 cut(s) 92, 637
Hpy99I CGWCG 3 cut(s) 52, 55, 258
HpyAV CCTTC 2 cut(s) 456, 842
HpyCH4III ACNGT 3 cut(s) 29, 532, 634
HpyCH4IV ACGT 2 cut(s) 50, 88
HpyCH4V TGCA 5 cut(s) 166, 464, 542, 596, 799
HpyF10VI GCNNNNNNNGC 6 cut(s) 166, 183, 373, 470, 926, 962
HpyF3I CTNAG 8 cut(s) 281, 288, 564, 640, 726, 777, 807, 829
HpySE526I ACGT 2 cut(s) 50, 88
Hsp92I GRCGYC 3 cut(s) 50, 134, 256
Hsp92II CATG 1 cut(s) 596
HspAI GCGC 3 cut(s) 194, 317, 927
KroI GCCGGC 1 cut(s) 923
KroNI GCCGGC 1 cut(s) 925
Kzo9I GATC 2 cut(s) 307, 894
LmnI GCTCC 2 cut(s) 174, 191
Lsp1109I GCAGC 7 cut(s) 147, 150, 178, 278, 376, 603, 850
LweI GCATC 1 cut(s) 550
MaeII ACGT 2 cut(s) 50, 88
MaeIII GTNAC 1 cut(s) 379
MalI GATC 2 cut(s) 309, 896
MboI GATC 2 cut(s) 307, 894
MboII GAAGA 3 cut(s) 441, 588, 813
MhlI GDGCHC 2 cut(s) 188, 289
MluCI AATT 4 cut(s) 20, 202, 345, 666
MlyI GAGTC 1 cut(s) 722
MmeI TCCRAC 2 cut(s) 126, 681
MnlI CCTC 8 cut(s) 52, 100, 116, 290, 469, 653, 721, 838
MroNI GCCGGC 1 cut(s) 923
MseI TTAA 2 cut(s) 17, 669
MslI CAYNNNNRTG 2 cut(s) 551, 632
MspI CCGG 2 cut(s) 377, 924
MspR9I CCNGG 1 cut(s) 653
MvaI CCWGG 1 cut(s) 653
MvnI CGCG 2 cut(s) 317, 920
MwoI GCNNNNNNNGC 6 cut(s) 166, 183, 373, 470, 926, 962
NaeI GCCGGC 1 cut(s) 925
NdeII GATC 2 cut(s) 307, 894
NgoMIV GCCGGC 1 cut(s) 923
NlaIII CATG 1 cut(s) 596
NmeAIII GCCGAG 3 cut(s) 162, 859, 868
NmuCI GTSAC 1 cut(s) 379
OliI CACNNNNGTG 1 cut(s) 632
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 124
PdiI GCCGGC 1 cut(s) 925
PfeI GAWTC 4 cut(s) 303, 441, 512, 890
PkrI GCNGC 9 cut(s) 162, 165, 168, 268, 340, 366, 618, 865, 922
Ple19I CGATCG 1 cut(s) 897
PleI GAGTC 1 cut(s) 721
PmaCI CACGTG 1 cut(s) 89
PmlI CACGTG 1 cut(s) 89
PpsI GAGTC 1 cut(s) 721
Ppu21I YACGTR 1 cut(s) 89
PsiI TTATAA 1 cut(s) 249
Psp124BI GAGCTC 2 cut(s) 188, 289
Psp6I CCWGG 1 cut(s) 651
PspCI CACGTG 1 cut(s) 89
PspFI CCCAGC 2 cut(s) 231, 434
PspGI CCWGG 1 cut(s) 651
PspPI GGNCC 2 cut(s) 502, 764
PstI CTGCAG 1 cut(s) 168
PstNI CAGNNNCTG 1 cut(s) 295
PvuI CGATCG 1 cut(s) 897
RsaI GTAC 2 cut(s) 40, 603
RsaNI GTAC 2 cut(s) 39, 602
RseI CAYNNNNRTG 2 cut(s) 551, 632
SacI GAGCTC 2 cut(s) 188, 289
SaqAI TTAA 2 cut(s) 17, 669
SatI GCNGC 9 cut(s) 161, 164, 167, 267, 339, 365, 617, 864, 921
Sau3AI GATC 2 cut(s) 307, 894
Sau96I GGNCC 2 cut(s) 502, 764
ScaI AGTACT 1 cut(s) 603
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 1 cut(s) 653
SduI GDGCHC 2 cut(s) 188, 289
SfaNI GCATC 1 cut(s) 550
SfcI CTRYAG 1 cut(s) 164
SmiMI CAYNNNNRTG 2 cut(s) 551, 632
Sse9I AATT 4 cut(s) 20, 202, 345, 666
SsiI CCGC 4 cut(s) 338, 422, 920, 956
SspI AATATT 1 cut(s) 519
SstI GAGCTC 2 cut(s) 188, 289
StyD4I CCNGG 1 cut(s) 651
StyI CCWWGG 3 cut(s) 170, 487, 783
TaaI ACNGT 3 cut(s) 29, 532, 634
TaiI ACGT 2 cut(s) 53, 91
TaqI TCGA 6 cut(s) 57, 111, 127, 182, 306, 893
TasI AATT 4 cut(s) 20, 202, 345, 666
TatI WGTACW 2 cut(s) 38, 601
TauI GCSGC 2 cut(s) 341, 923
TfiI GAWTC 4 cut(s) 303, 441, 512, 890
Tru1I TTAA 2 cut(s) 17, 669
Tru9I TTAA 2 cut(s) 17, 669
TscAI CASTG 1 cut(s) 910
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 7 cut(s) 160, 163, 166, 266, 364, 616, 863
Tsp45I GTSAC 1 cut(s) 379
TspGWI ACGGA 2 cut(s) 315, 672
TspRI CASTG 1 cut(s) 910
XagI CCTNNNNNAGG 1 cut(s) 497
XapI RAATTY 1 cut(s) 345
ZraI GACGTC 1 cut(s) 51
ZrmI AGTACT 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.