pycom05g23330

Transcription initiation factor TFIID subunit 8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006787
Physical Location & Seq
Reverse (-)
25226741 .. 25227631
891 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 804 bp
ATGTCCCAAAAACCCAAATCACGACGCAAAACCACAAAAATCGAACAAACCCCAACACTCCCAATCCAAAACCCGACCCCACCAGAGTTCCATTTTGCCATAGCCAGGATCGCGGTGTCTCAGATCAGCCAATCAGTCGGATTCAAATCAACGAAGCCCTCCGCACTCGAAACCCTGACCCACATCGCCGTCAAGTACATCGGCGCCGTAGCCTCCGCCTCGGCCTCCTACGCCGCCATGGCCAAGCGCACCAACACGAACGTCTTCGACTTCGCCAACGCCCTCCACGACTTGCAGTCGGTGCAGGGATTCAGAGGCGCCTCCGCCCTCCACGAAAACGGATTCTGCGTATTGGGTTCGAGCGTTTTGAGCGATCTCGCCGGGTTCGTGAGGTGGAATCGGGAAACCCCCTTCGCCAGGCCGATTCCGCGGGTGGAGAAATTGGGCGAGGAGAAAAGGGGAAATGGGGTTCCCATTCCGCCGGAACTGGATTCCCGGAGGGGCTTGCACGTGCCGAGATGGCTGCCGGCGTTTCCGGACATGGGGAAGGAGGTTGGGAAGAGGAGGAATGGGGAGGAGCTGTGGGAGAATAGTAAGAGGGGGAGAGTGAGGTTTAGGGTCGGAGGAGGGGAGGAGGAGAGTGGGGTGGTGGGGGGAATGGGTGTGGATTTGAGGAGCGGGGTGTGTAGAGGAGGGAAGAGGGTGTCTTGTAGTAACAGTAAAATTGGTTACACTAATTTCAGTACCAGAACAAGAATTCATGAATTTGTTGATGATGATGATGATGATGGTGATAAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
Bromo_TP TAF8_C

Protein Analysis

268

Amino Acids

29.32

Weight (kDa)

9.79

Isoelectric Point (pI)

51.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bromo_TP PF07524 29 - 98 1.7e-16 Bromodomain associated
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 203, 317
AccBSI CCGCTC 1 cut(s) 678
AccII CGCG 2 cut(s) 113, 430
AccIII TCCGGA 1 cut(s) 535
AciI CCGC 9 cut(s) 113, 162, 216, 234, 324, 428, 430, 479, 678
AclWI GGATC 1 cut(s) 116
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 2 cut(s) 756, 764
AcvI CACGTG 1 cut(s) 511
AcyI GRCGYC 2 cut(s) 204, 318
AfaI GTAC 2 cut(s) 197, 745
AfiI CCNNNNNNNGG 3 cut(s) 105, 417, 542
AgsI TTSAA 1 cut(s) 145
AhdI GACNNNNNGTC 1 cut(s) 295
AjnI CCWGG 2 cut(s) 104, 416
AloI GAACNNNNNNTCC 2 cut(s) 453, 485
AluBI AGCT 1 cut(s) 580
AluI AGCT 1 cut(s) 580
Alw26I GTCTC 1 cut(s) 123
AlwI GGATC 1 cut(s) 116
Aor13HI TCCGGA 1 cut(s) 535
AoxI GGCC 3 cut(s) 222, 240, 419
ApeKI GCWGC 1 cut(s) 523
ApoI RAATTY 2 cut(s) 756, 764
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 3 cut(s) 206, 249, 320
AsuC2I CCSGG 2 cut(s) 382, 496
AsuHPI GGTGA 1 cut(s) 803
BalI TGGCCA 1 cut(s) 242
BanI GGYRCC 2 cut(s) 203, 317
BbrPI CACGTG 1 cut(s) 511
BbsI GAAGAC 1 cut(s) 256
BbvI GCAGC 1 cut(s) 510
BccI CCATC 2 cut(s) 513, 782
BceAI ACGGC 2 cut(s) 173, 191
BcgI CGANNNNNNTGC 2 cut(s) 505, 539
BciT130I CCWGG 2 cut(s) 106, 418
BcnI CCSGG 2 cut(s) 382, 496
BcoDI GTCTC 1 cut(s) 123
BfoI RGCGCY 2 cut(s) 207, 321
BglI GCCNNNNNGGC 2 cut(s) 239, 520
BisI GCNGC 2 cut(s) 234, 524
BlsI GCNGC 2 cut(s) 235, 525
Bme1390I CCNGG 4 cut(s) 106, 382, 418, 496
BmeRI GACNNNNNGTC 1 cut(s) 295
BmiI GGNNCC 3 cut(s) 205, 319, 471
BmrFI CCNGG 4 cut(s) 106, 382, 418, 496
BpiI GAAGAC 1 cut(s) 256
BpuMI CCSGG 2 cut(s) 382, 496
BsaAI YACGTR 1 cut(s) 511
BsaBI GATNNNNATC 1 cut(s) 145
BsaHI GRCGYC 2 cut(s) 204, 318
BsaJI CCNNGG 3 cut(s) 219, 237, 428
BsaWI WCCGGW 1 cut(s) 535
BsaXI ACNNNNNCTCC 2 cut(s) 566, 596
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 417, 542
Bse118I RCCGGY 1 cut(s) 526
Bse1I ACTGG 1 cut(s) 492
Bse8I GATNNNNATC 1 cut(s) 145
BseAI TCCGGA 1 cut(s) 535
BseBI CCWGG 2 cut(s) 106, 418
BseDI CCNNGG 3 cut(s) 219, 237, 428
BseJI GATNNNNATC 1 cut(s) 145
BseLI CCNNNNNNNGG 3 cut(s) 105, 417, 542
BseMII CTCAG 1 cut(s) 134
BseNI ACTGG 1 cut(s) 492
BseRI GAGGAG 8 cut(s) 464, 577, 590, 639, 647, 650, 688, 705
BseXI GCAGC 1 cut(s) 510
BsgI GTGCAG 1 cut(s) 323
Bsh1236I CGCG 2 cut(s) 113, 430
BshFI GGCC 3 cut(s) 224, 242, 421
BshNI GGYRCC 2 cut(s) 203, 317
BsiSI CCGG 5 cut(s) 381, 482, 496, 527, 536
BslI CCNNNNNNNGG 3 cut(s) 105, 417, 542
BsmAI GTCTC 1 cut(s) 123
BsnI GGCC 3 cut(s) 224, 242, 421
Bsp13I TCCGGA 1 cut(s) 535
Bsp143I GATC 3 cut(s) 108, 123, 373
Bsp19I CCATGG 1 cut(s) 237
BspACI CCGC 9 cut(s) 113, 162, 216, 234, 324, 428, 430, 479, 678
BspANI GGCC 3 cut(s) 224, 242, 421
BspCNI CTCAG 1 cut(s) 133
BspEI TCCGGA 1 cut(s) 535
BspFNI CGCG 2 cut(s) 113, 430
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 3 cut(s) 205, 319, 471
BspPI GGATC 1 cut(s) 116
BspT107I GGYRCC 2 cut(s) 203, 317
BsrBI CCGCTC 1 cut(s) 678
BsrFI RCCGGY 1 cut(s) 526
BsrI ACTGG 1 cut(s) 492
BssAI RCCGGY 1 cut(s) 526
BssECI CCNNGG 3 cut(s) 219, 237, 428
BssMI GATC 3 cut(s) 108, 123, 373
BssNI GRCGYC 2 cut(s) 204, 318
BssT1I CCWWGG 1 cut(s) 237
Bst2UI CCWGG 2 cut(s) 106, 418
Bst4CI ACNGT 1 cut(s) 719
Bst6I CTCTTC 2 cut(s) 554, 692
BstACI GRCGYC 2 cut(s) 204, 318
BstAPI GCANNNNNTGC 1 cut(s) 301
BstBAI YACGTR 1 cut(s) 511
BstC8I GCNNGC 2 cut(s) 506, 528
BstDEI CTNAG 1 cut(s) 120
BstDSI CCRYGG 2 cut(s) 237, 428
BstENI CCTNNNNNAGG 1 cut(s) 415
BstFNI CGCG 2 cut(s) 113, 430
BstH2I RGCGCY 2 cut(s) 207, 321
BstHHI GCGC 3 cut(s) 206, 249, 320
BstKTI GATC 3 cut(s) 111, 126, 376
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 3 cut(s) 108, 123, 373
BstMWI GCNNNNNNNGC 7 cut(s) 110, 230, 239, 301, 369, 427, 520
BstNI CCWGG 2 cut(s) 106, 418
BstSCI CCNGG 4 cut(s) 104, 380, 416, 494
BstUI CGCG 2 cut(s) 113, 430
BstV1I GCAGC 1 cut(s) 510
BstV2I GAAGAC 1 cut(s) 256
BsuRI GGCC 3 cut(s) 224, 242, 421
BtgI CCRYGG 2 cut(s) 237, 428
BtgZI GCGATG 1 cut(s) 169
Cac8I GCNNGC 2 cut(s) 506, 528
CciI TCATGA 1 cut(s) 760
CfoI GCGC 3 cut(s) 206, 249, 320
Cfr10I RCCGGY 1 cut(s) 526
Cfr42I CCGCGG 1 cut(s) 431
CseI GACGC 1 cut(s) 33
Csp6I GTAC 2 cut(s) 196, 744
CviAII CATG 3 cut(s) 238, 541, 761
CviQI GTAC 2 cut(s) 196, 744
DdeI CTNAG 1 cut(s) 120
DinI GGCGCC 2 cut(s) 205, 319
DpnI GATC 3 cut(s) 110, 125, 375
DpnII GATC 3 cut(s) 108, 123, 373
DriI GACNNNNNGTC 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 240
Eam1104I CTCTTC 2 cut(s) 554, 692
Eam1105I GACNNNNNGTC 1 cut(s) 295
EarI CTCTTC 2 cut(s) 554, 692
EciI GGCGGA 3 cut(s) 205, 313, 468
Eco130I CCWWGG 1 cut(s) 237
Eco72I CACGTG 1 cut(s) 511
EcoNI CCTNNNNNAGG 1 cut(s) 415
EcoRI GAATTC 1 cut(s) 756
EcoRII CCWGG 2 cut(s) 104, 416
EcoT14I CCWWGG 1 cut(s) 237
EgeI GGCGCC 2 cut(s) 205, 319
EheI GGCGCC 2 cut(s) 205, 319
ErhI CCWWGG 1 cut(s) 237
FaeI CATG 3 cut(s) 241, 544, 764
FaiI YATR 4 cut(s) 101, 239, 542, 762
FatI CATG 3 cut(s) 237, 540, 760
FauI CCCGC 2 cut(s) 423, 671
Fnu4HI GCNGC 2 cut(s) 234, 524
Fsp4HI GCNGC 2 cut(s) 234, 524
GlaI GCGC 3 cut(s) 205, 248, 319
GluI GCNGC 2 cut(s) 234, 524
HaeII RGCGCY 2 cut(s) 207, 321
HaeIII GGCC 3 cut(s) 224, 242, 421
HapII CCGG 5 cut(s) 381, 482, 496, 527, 536
HgaI GACGC 1 cut(s) 33
HhaI GCGC 3 cut(s) 206, 249, 320
Hin1I GRCGYC 2 cut(s) 204, 318
Hin1II CATG 3 cut(s) 241, 544, 764
Hin6I GCGC 3 cut(s) 204, 247, 318
HinP1I GCGC 3 cut(s) 204, 247, 318
HinfI GANTC 6 cut(s) 141, 309, 342, 397, 424, 491
HpaII CCGG 5 cut(s) 381, 482, 496, 527, 536
HphI GGTGA 1 cut(s) 803
Hpy188I TCNGA 4 cut(s) 123, 140, 314, 623
Hpy188III TCNNGA 5 cut(s) 21, 388, 401, 536, 761
Hpy99I CGWCG 1 cut(s) 27
HpyAV CCTTC 2 cut(s) 421, 541
HpyCH4III ACNGT 1 cut(s) 719
HpyCH4IV ACGT 2 cut(s) 261, 510
HpyCH4V TGCA 3 cut(s) 295, 304, 508
HpyF10VI GCNNNNNNNGC 7 cut(s) 110, 230, 239, 301, 369, 427, 520
HpyF3I CTNAG 1 cut(s) 120
HpySE526I ACGT 2 cut(s) 261, 510
Hsp92I GRCGYC 2 cut(s) 204, 318
Hsp92II CATG 3 cut(s) 241, 544, 764
HspAI GCGC 3 cut(s) 204, 247, 318
KasI GGCGCC 2 cut(s) 203, 317
Kpn2I TCCGGA 1 cut(s) 535
KroI GCCGGC 1 cut(s) 526
KroNI GCCGGC 1 cut(s) 528
KspI CCGCGG 1 cut(s) 431
Kzo9I GATC 3 cut(s) 108, 123, 373
LmnI GCTCC 2 cut(s) 577, 675
Lsp1109I GCAGC 1 cut(s) 510
MaeII ACGT 2 cut(s) 261, 510
MaeIII GTNAC 2 cut(s) 713, 728
MalI GATC 3 cut(s) 110, 125, 375
MbiI CCGCTC 1 cut(s) 678
MboI GATC 3 cut(s) 108, 123, 373
MboII GAAGA 3 cut(s) 256, 571, 709
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 5 cut(s) 440, 723, 736, 756, 764
MluNI TGGCCA 1 cut(s) 242
Mly113I GGCGCC 2 cut(s) 204, 318
MmeI TCCRAC 2 cut(s) 118, 601
Mox20I TGGCCA 1 cut(s) 242
MroI TCCGGA 1 cut(s) 535
MroNI GCCGGC 1 cut(s) 526
MroXI GAANNNNTTC 1 cut(s) 263
MscI TGGCCA 1 cut(s) 242
Msp20I TGGCCA 1 cut(s) 242
MspA1I CMGCKG 1 cut(s) 430
MspI CCGG 5 cut(s) 381, 482, 496, 527, 536
MspR9I CCNGG 4 cut(s) 106, 382, 418, 496
MvaI CCWGG 2 cut(s) 106, 418
MvnI CGCG 2 cut(s) 113, 430
MwoI GCNNNNNNNGC 7 cut(s) 110, 230, 239, 301, 369, 427, 520
NaeI GCCGGC 1 cut(s) 528
NarI GGCGCC 2 cut(s) 204, 318
NciI CCSGG 2 cut(s) 382, 496
NcoI CCATGG 1 cut(s) 237
NdeII GATC 3 cut(s) 108, 123, 373
NgoMIV GCCGGC 1 cut(s) 526
NlaIII CATG 3 cut(s) 241, 544, 764
NlaIV GGNNCC 3 cut(s) 205, 319, 471
NmeAIII GCCGAG 2 cut(s) 200, 540
PagI TCATGA 1 cut(s) 760
PcsI WCGNNNNNNNCGW 3 cut(s) 285, 345, 384
PdiI GCCGGC 1 cut(s) 528
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 6 cut(s) 141, 309, 342, 397, 424, 491
PfoI TCCNGGA 1 cut(s) 494
PkrI GCNGC 2 cut(s) 235, 525
PluTI GGCGCC 2 cut(s) 207, 321
PmaCI CACGTG 1 cut(s) 511
PmlI CACGTG 1 cut(s) 511
Ppu21I YACGTR 1 cut(s) 511
Psp6I CCWGG 2 cut(s) 104, 416
PspCI CACGTG 1 cut(s) 511
PspGI CCWGG 2 cut(s) 104, 416
PspN4I GGNNCC 3 cut(s) 205, 319, 471
RsaI GTAC 2 cut(s) 197, 745
RsaNI GTAC 2 cut(s) 196, 744
SacII CCGCGG 1 cut(s) 431
SatI GCNGC 2 cut(s) 234, 524
Sau3AI GATC 3 cut(s) 108, 123, 373
ScrFI CCNGG 4 cut(s) 106, 382, 418, 496
SetI ASST 6 cut(s) 264, 395, 513, 555, 582, 614
SfoI GGCGCC 2 cut(s) 205, 319
Sfr303I CCGCGG 1 cut(s) 431
SgrBI CCGCGG 1 cut(s) 431
Sse9I AATT 5 cut(s) 440, 723, 736, 756, 764
SsiI CCGC 9 cut(s) 113, 162, 216, 234, 324, 428, 430, 479, 678
SspDI GGCGCC 2 cut(s) 203, 317
StyD4I CCNGG 4 cut(s) 104, 380, 416, 494
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 1 cut(s) 719
TaiI ACGT 2 cut(s) 264, 513
TaqI TCGA 4 cut(s) 42, 168, 267, 359
TasI AATT 5 cut(s) 440, 723, 736, 756, 764
TatI WGTACW 1 cut(s) 195
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 6 cut(s) 141, 309, 342, 397, 424, 491
TseI GCWGC 1 cut(s) 523
TspDTI ATGAA 2 cut(s) 749, 777
TspGWI ACGGA 1 cut(s) 354
XagI CCTNNNNNAGG 1 cut(s) 415
XapI RAATTY 2 cut(s) 756, 764
XmnI GAANNNNTTC 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.