pycom05g27470
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011269
Physical Location & Seq
Reverse (-)
28440107 .. 28441306
1200 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 840 bp
ATGGACCCCACATTCTTCAGCAGCAACAGCAAACAGTTTCTGAGCGATCAGGAAGAGAACGACAACACCACCAGCTCCACGCCGGAAAACAGCTCAGACTCTCCTCCGCCGTCCACCAATTTCAGTGACTACTCCAAGATCACCACTTCCACCTCCTCCCCCAAAAAAAGTGGTCGGCGAGCAATACAGAAACGAGTGGTGTCGGTCCCCATCAAAGGCGACAACAGCAACAGCAACACTCCGCCGCCGTCCGATTCTTGGGCTTGGAGAAAGTACGGCCAAAAACCCATCAAAGGCTCACCTTATCCCAGAGGGTATTACAGGTGCAGTAGCTCAAAAGGATGCCCGGCGAGGAAACAAGTAGAGAGAAGCCGCGTGGACCCCACCACGCTGGTCATCACCTACTCCTCCGAACACAACCACTCTTGGCTCGCCTCTCGCAACCACCACAACAACCACAACAATTCAAGTTCCGCCGCGGCAACCAAACGCGGCCCCACCAAGACCGAAGCCCCGGAGGCCCAGCAACCCGACCACCAAGATCAAGACCTAGACCAAGACCAAGACTCTAGGTTCGCGGATCTAAACCACGAGTCGCTTATCGTAAACGACGAGTTCGTCTGGTTCGCCGACATGGAGACCACGTCGTCGAGGATGCTCGAGAGCCCCATTTTCGCCGAGAGCAGCAGCTCAGTCGGGTCCGACTCCGGGGACAAGGCGGCGATGGTGTTCCCCATGGGAGAGGAGGACGAGTCGCTCTTCGCCGATCTGGGCGAGCTGCCGGAGTGCACTTTCGTGTTTAGGTACCGTGGTGTGGGACCACAAGCTCAGATCTGTTGA

Protein Analysis

280

Amino Acids

30.92

Weight (kDa)

5.3

Isoelectric Point (pI)

57.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 85 - 141 1.2e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29280
fragaria_vesca FvH4_3g07360 FvH4_3g07360
malus_domestica MD05G1295700.v1.1 MD10G1275800.v1.1
prunus_persica Prupe.4G066400_v2.0.a1
pyrus_communis pycom05g27470 pycom10g23050
rosa_chinensis RchiOBHm_Chr5g0011581
rosa_laevigata RLG00000031844
rosa_multiflora Rmu_sc0009001.1_g000011 Rmu_sc0040970.1_g000001
rosa_roxburghii Rroxscaffold_1G00064910
rosa_rugosa Rorug04G0457800 Rorug04G0457800
rosa_samantha Rh5AG089500 Rh5BG084800 Rh5CG098900 Rh5DG084800
rosa_wichuraiana Rw5G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 617, 646
Acc65I GGTACC 1 cut(s) 804
AccB1I GGYRCC 1 cut(s) 804
AccII CGCG 4 cut(s) 375, 479, 492, 578
AclWI GGATC 1 cut(s) 588
AcoI YGGCCR 1 cut(s) 277
AfaI GTAC 2 cut(s) 275, 806
AfiI CCNNNNNNNGG 5 cut(s) 215, 258, 293, 708, 814
AgsI TTSAA 1 cut(s) 468
AjiI CACGTC 1 cut(s) 645
AleI CACNNNNGTG 1 cut(s) 794
AluBI AGCT 6 cut(s) 75, 93, 333, 690, 778, 827
AluI AGCT 6 cut(s) 75, 93, 333, 690, 778, 827
Alw21I GWGCWC 1 cut(s) 791
Alw26I GTCTC 1 cut(s) 632
Alw44I GTGCAC 1 cut(s) 787
AlwI GGATC 1 cut(s) 588
AlwNI CAGNNNCTG 1 cut(s) 40
Ama87I CYCGRG 1 cut(s) 659
AoxI GGCC 3 cut(s) 277, 493, 519
ApaLI GTGCAC 1 cut(s) 787
ApeKI GCWGC 4 cut(s) 21, 684, 687, 778
ArsI GACNNNNNNTTYG 2 cut(s) 557, 589
Asp718I GGTACC 1 cut(s) 804
AspS9I GGNCC 7 cut(s) 4, 205, 379, 494, 520, 699, 818
AsuC2I CCSGG 3 cut(s) 347, 515, 709
AsuHPI GGTGA 3 cut(s) 133, 291, 391
AvaI CYCGRG 1 cut(s) 659
AvaII GGWCC 5 cut(s) 4, 205, 379, 699, 818
BaeGI GKGCMC 1 cut(s) 791
BaeI ACNNNNGTAYC 2 cut(s) 796, 829
BanI GGYRCC 1 cut(s) 804
BanII GRGCYC 1 cut(s) 668
BauI CACGAG 1 cut(s) 590
Bbv12I GWGCWC 1 cut(s) 791
BbvI GCAGC 4 cut(s) 33, 696, 699, 765
BccI CCATC 3 cut(s) 218, 296, 718
BceAI ACGGC 3 cut(s) 94, 232, 292
BcgI CGANNNNNNTGC 2 cut(s) 676, 710
BcnI CCSGG 3 cut(s) 347, 515, 709
BcoDI GTCTC 1 cut(s) 632
BfaI CTAG 2 cut(s) 551, 570
BglI GCCNNNNNGGC 1 cut(s) 518
BglII AGATCT 1 cut(s) 831
Bme1390I CCNGG 3 cut(s) 347, 515, 709
Bme18I GGWCC 5 cut(s) 4, 205, 379, 699, 818
BmeT110I CYCGRG 1 cut(s) 659
BmgBI CACGTC 1 cut(s) 645
BmgT120I GGNCC 7 cut(s) 4, 205, 379, 494, 520, 699, 818
BmiI GGNNCC 7 cut(s) 6, 207, 381, 496, 700, 806, 819
BmrFI CCNGG 3 cut(s) 347, 515, 709
BmsI GCATC 2 cut(s) 332, 645
BpuMI CCSGG 3 cut(s) 347, 515, 709
BsaI GGTCTC 1 cut(s) 632
BsaJI CCNNGG 5 cut(s) 477, 513, 708, 735, 808
BsaXI ACNNNNNCTCC 4 cut(s) 629, 659, 737, 767
Bsc4I CCNNNNNNNGG 5 cut(s) 215, 258, 293, 708, 814
BseDI CCNNGG 5 cut(s) 477, 513, 708, 735, 808
BseGI GGATG 2 cut(s) 347, 660
BseLI CCNNNNNNNGG 5 cut(s) 215, 258, 293, 708, 814
BseMII CTCAG 3 cut(s) 32, 108, 705
BseRI GAGGAG 4 cut(s) 93, 145, 397, 758
BseSI GKGCMC 1 cut(s) 791
BseXI GCAGC 4 cut(s) 33, 696, 699, 765
BseYI CCCAGC 1 cut(s) 522
BsgI GTGCAG 1 cut(s) 346
Bsh1236I CGCG 4 cut(s) 375, 479, 492, 578
BshFI GGCC 3 cut(s) 279, 495, 521
BshNI GGYRCC 1 cut(s) 804
BsiHKAI GWGCWC 1 cut(s) 791
BsiHKCI CYCGRG 1 cut(s) 659
BsiSI CCGG 5 cut(s) 83, 347, 515, 708, 782
BslFI GGGAC 3 cut(s) 191, 725, 831
BslI CCNNNNNNNGG 5 cut(s) 215, 258, 293, 708, 814
BsmAI GTCTC 1 cut(s) 632
BsmFI GGGAC 3 cut(s) 191, 725, 831
BsnI GGCC 3 cut(s) 279, 495, 521
Bso31I GGTCTC 1 cut(s) 632
BsoBI CYCGRG 1 cut(s) 659
Bsp1286I GDGCHC 2 cut(s) 668, 791
Bsp143I GATC 6 cut(s) 46, 138, 541, 580, 766, 831
Bsp19I CCATGG 1 cut(s) 735
BspANI GGCC 3 cut(s) 279, 495, 521
BspCNI CTCAG 3 cut(s) 33, 107, 704
BspFNI CGCG 4 cut(s) 375, 479, 492, 578
BspLI GGNNCC 7 cut(s) 6, 207, 381, 496, 700, 806, 819
BspPI GGATC 1 cut(s) 588
BspQI GCTCTTC 1 cut(s) 764
BspT107I GGYRCC 1 cut(s) 804
BspTNI GGTCTC 1 cut(s) 632
BssECI CCNNGG 5 cut(s) 477, 513, 708, 735, 808
BssMI GATC 6 cut(s) 46, 138, 541, 580, 766, 831
BssSI CACGAG 1 cut(s) 590
BssT1I CCWWGG 1 cut(s) 735
Bst2BI CACGAG 1 cut(s) 590
Bst4CI ACNGT 2 cut(s) 36, 809
Bst6I CTCTTC 2 cut(s) 48, 764
BstC8I GCNNGC 3 cut(s) 180, 432, 776
BstDEI CTNAG 4 cut(s) 41, 94, 691, 828
BstDSI CCRYGG 3 cut(s) 477, 735, 808
BstF5I GGATG 2 cut(s) 347, 660
BstFNI CGCG 4 cut(s) 375, 479, 492, 578
BstKTI GATC 6 cut(s) 49, 141, 544, 583, 769, 834
BstMAI GTCTC 1 cut(s) 632
BstMBI GATC 6 cut(s) 46, 138, 541, 580, 766, 831
BstMWI GCNNNNNNNGC 3 cut(s) 27, 225, 518
BstSCI CCNGG 3 cut(s) 345, 513, 707
BstSLI GKGCMC 1 cut(s) 791
BstUI CGCG 4 cut(s) 375, 479, 492, 578
BstV1I GCAGC 4 cut(s) 33, 696, 699, 765
BstX2I RGATCY 2 cut(s) 580, 831
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 2 cut(s) 580, 831
BsuRI GGCC 3 cut(s) 279, 495, 521
BtgI CCRYGG 3 cut(s) 477, 735, 808
BtgZI GCGATG 1 cut(s) 737
BtrI CACGTC 1 cut(s) 645
BtsCI GGATG 2 cut(s) 347, 660
BtsIMutI CAGTG 1 cut(s) 130
Cac8I GCNNGC 3 cut(s) 180, 432, 776
CaiI CAGNNNCTG 1 cut(s) 40
Cfr13I GGNCC 7 cut(s) 4, 205, 379, 494, 520, 699, 818
Cfr42I CCGCGG 1 cut(s) 480
Csp6I GTAC 2 cut(s) 274, 805
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviAII CATG 2 cut(s) 634, 736
CviQI GTAC 2 cut(s) 274, 805
DdeI CTNAG 4 cut(s) 41, 94, 691, 828
DpnI GATC 6 cut(s) 48, 140, 543, 582, 768, 833
DpnII GATC 6 cut(s) 46, 138, 541, 580, 766, 831
DrdI GACNNNNNNGTC 2 cut(s) 617, 646
DseDI GACNNNNNNGTC 2 cut(s) 617, 646
EaeI YGGCCR 1 cut(s) 277
Eam1104I CTCTTC 2 cut(s) 48, 764
EarI CTCTTC 2 cut(s) 48, 764
EciI GGCGGA 3 cut(s) 96, 231, 463
Eco130I CCWWGG 1 cut(s) 735
Eco24I GRGCYC 1 cut(s) 668
Eco31I GGTCTC 1 cut(s) 632
Eco47I GGWCC 5 cut(s) 4, 205, 379, 699, 818
Eco88I CYCGRG 1 cut(s) 659
EcoT14I CCWWGG 1 cut(s) 735
EcoT38I GRGCYC 1 cut(s) 668
ErhI CCWWGG 1 cut(s) 735
FaeI CATG 2 cut(s) 637, 739
FaiI YATR 2 cut(s) 635, 737
FaqI GGGAC 3 cut(s) 191, 725, 831
FatI CATG 2 cut(s) 633, 735
FokI GGATG 2 cut(s) 354, 667
FriOI GRGCYC 1 cut(s) 668
FspBI CTAG 2 cut(s) 551, 570
GsaI CCCAGC 1 cut(s) 526
HaeIII GGCC 3 cut(s) 279, 495, 521
HapII CCGG 5 cut(s) 83, 347, 515, 708, 782
Hin1II CATG 2 cut(s) 637, 739
HinfI GANTC 6 cut(s) 98, 254, 566, 593, 704, 752
HpaII CCGG 5 cut(s) 83, 347, 515, 708, 782
HphI GGTGA 3 cut(s) 133, 291, 391
Hpy166II GTNNAC 4 cut(s) 114, 379, 607, 789
Hpy188I TCNGA 6 cut(s) 42, 97, 253, 412, 703, 831
Hpy188III TCNNGA 3 cut(s) 50, 545, 661
Hpy8I GTNNAC 4 cut(s) 114, 379, 607, 789
Hpy99I CGWCG 3 cut(s) 614, 649, 652
HpyCH4III ACNGT 2 cut(s) 36, 809
HpyCH4IV ACGT 1 cut(s) 644
HpyCH4V TGCA 2 cut(s) 327, 789
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 225, 518
HpyF3I CTNAG 4 cut(s) 41, 94, 691, 828
HpySE526I ACGT 1 cut(s) 644
Hsp92II CATG 2 cut(s) 637, 739
KpnI GGTACC 1 cut(s) 808
KspI CCGCGG 1 cut(s) 480
Kzo9I GATC 6 cut(s) 46, 138, 541, 580, 766, 831
LguI GCTCTTC 1 cut(s) 764
LmnI GCTCC 1 cut(s) 80
Lsp1109I GCAGC 4 cut(s) 33, 696, 699, 765
LweI GCATC 2 cut(s) 332, 645
MaeI CTAG 2 cut(s) 551, 570
MaeII ACGT 1 cut(s) 644
MaeIII GTNAC 1 cut(s) 125
MalI GATC 6 cut(s) 48, 140, 543, 582, 768, 833
MboI GATC 6 cut(s) 46, 138, 541, 580, 766, 831
MboII GAAGA 3 cut(s) 7, 65, 751
MflI RGATCY 2 cut(s) 580, 831
MhlI GDGCHC 2 cut(s) 668, 791
MluCI AATT 2 cut(s) 118, 463
MlyI GAGTC 5 cut(s) 92, 560, 602, 698, 761
MmeI TCCRAC 1 cut(s) 726
MslI CAYNNNNRTG 1 cut(s) 794
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 5 cut(s) 83, 347, 515, 708, 782
MspR9I CCNGG 3 cut(s) 347, 515, 709
MvnI CGCG 4 cut(s) 375, 479, 492, 578
MwoI GCNNNNNNNGC 3 cut(s) 27, 225, 518
NciI CCSGG 3 cut(s) 347, 515, 709
NcoI CCATGG 1 cut(s) 735
NdeII GATC 6 cut(s) 46, 138, 541, 580, 766, 831
NlaIII CATG 2 cut(s) 637, 739
NlaIV GGNNCC 7 cut(s) 6, 207, 381, 496, 700, 806, 819
NmeAIII GCCGAG 1 cut(s) 703
NmuCI GTSAC 1 cut(s) 125
OliI CACNNNNGTG 1 cut(s) 794
PaeR7I CTCGAG 1 cut(s) 659
PciSI GCTCTTC 1 cut(s) 764
PcsI WCGNNNNNNNCGW 2 cut(s) 609, 615
PfeI GAWTC 1 cut(s) 254
PflFI GACNNNGTC 1 cut(s) 643
PleI GAGTC 5 cut(s) 92, 560, 601, 698, 760
PpsI GAGTC 5 cut(s) 92, 560, 601, 698, 760
PspFI CCCAGC 1 cut(s) 522
PspN4I GGNNCC 7 cut(s) 6, 207, 381, 496, 700, 806, 819
PspPI GGNCC 7 cut(s) 4, 205, 379, 494, 520, 699, 818
PstNI CAGNNNCTG 1 cut(s) 40
PsuI RGATCY 2 cut(s) 580, 831
PsyI GACNNNGTC 1 cut(s) 643
RsaI GTAC 2 cut(s) 275, 806
RsaNI GTAC 2 cut(s) 274, 805
RseI CAYNNNNRTG 1 cut(s) 794
SacII CCGCGG 1 cut(s) 480
SapI GCTCTTC 1 cut(s) 764
Sau3AI GATC 6 cut(s) 46, 138, 541, 580, 766, 831
Sau96I GGNCC 7 cut(s) 4, 205, 379, 494, 520, 699, 818
SchI GAGTC 5 cut(s) 92, 560, 602, 698, 761
ScrFI CCNGG 3 cut(s) 347, 515, 709
SduI GDGCHC 2 cut(s) 668, 791
SfaNI GCATC 2 cut(s) 332, 645
Sfr274I CTCGAG 1 cut(s) 659
Sfr303I CCGCGG 1 cut(s) 480
SgrBI CCGCGG 1 cut(s) 480
SinI GGWCC 5 cut(s) 4, 205, 379, 699, 818
SlaI CTCGAG 1 cut(s) 659
SmiMI CAYNNNNRTG 1 cut(s) 794
SmlI CTYRAG 1 cut(s) 659
SmoI CTYRAG 1 cut(s) 659
Sse9I AATT 2 cut(s) 118, 463
SspMI CTAG 2 cut(s) 551, 570
StyD4I CCNGG 3 cut(s) 345, 513, 707
StyI CCWWGG 1 cut(s) 735
TaaI ACNGT 2 cut(s) 36, 809
TaiI ACGT 1 cut(s) 647
TaqI TCGA 2 cut(s) 650, 660
TaqII GACCGA 2 cut(s) 193, 521
TasI AATT 2 cut(s) 118, 463
TauI GCSGC 6 cut(s) 247, 375, 479, 482, 495, 722
TfiI GAWTC 1 cut(s) 254
TscAI CASTG 1 cut(s) 130
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 4 cut(s) 21, 684, 687, 778
Tsp45I GTSAC 1 cut(s) 125
TspRI CASTG 1 cut(s) 130
Tth111I GACNNNGTC 1 cut(s) 643
VneI GTGCAC 1 cut(s) 787
VpaK11BI GGWCC 5 cut(s) 4, 205, 379, 699, 818
XhoI CTCGAG 1 cut(s) 659
XspI CTAG 2 cut(s) 551, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.