pycom05g27640

HCNGP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008773
Physical Location & Seq
Reverse (-)
28563797 .. 28566708
2912 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1368 bp
ATGGCATCGAGGAAGAAGCAATCCGATGCCATAGCTCTCCTGCTTTCCAATTACAACGACGAAGAAGAAGATGAAGACATGGAGGACGTTGAGCAGCACAACAACAAAGAAGAAGAAGGAGATGATGAGCGACGAGAACGGAGAGAATTCGACGATTACGGTGAGCTGAGAGGCTCGGGAGATGCTGCAATGGCGGATGTGGATGGAACGGTGGGTGGTGAGTCTGGGAACGATGATAGCGCACCGCCGAATGCCGGGGATAATGAGGTGGCTTCGGATGGTTTGAATAGAAGAAGAAGAGGGGCGCTTACGATTGTGGACTACGGGCATGATGAAGTTACTATGTCTCCTGAGCCTGAGGAGGGTGGAATAGAAGGGATTGGTCGTGTTCCTGGTGATTTCCATGATACGACATCTCCAGGAACAGTTCGCACTCCACAATTATCTGAACCGTCAAACTCTGACACAATGAACGATACTGTACTTGAATCTGAGGGTAGACATGCTGCTGAGGCTGTTGCAGAAGAGCAGAAAGATATTGGTCCGTTGGATAAGTTTATCCCGCCACCGTTAAAAGCAAAGTGTTCAGAGGAACTGCAAAGGAGGATAAATAAGTTTCTTGAATACAAGAGAGCTGGGAAAAGCTTCAATGCAGAAGTACGTAATAAGAAGGACTATAGGAATCCAAACTTTCTGGTGCATGCAGTGAGGTATCAAGATATTGATCAGATAGGGTCTTGCTTCAGTAAAGATGTATTTGACCCTCATGGATATGACAAAAGTGACTACTGTGATGAAATAGAGGCTGATATGAGGCATGAAATGGAAAGGAAAGAGCAAGATAGGAAGAAGAGTCAAAAGGTTGAATATGTTTCTGGAGGAACACAGCCTGGAATAGCCGGTGTTGCACCTAAAATTAACATACCTTATCCAGGTGTTTCAACCATGGCTGCTACTGGGTTGAACTCCTTACCGCCTGCAGCTGATGTTATCCCTCGGGAGGGTAGACAGAACAAGAAGTCAAAATGGGATAAGGTCGATGGTGATCGAAAAAATCCTCTTCCCTCAGGACTGCAGGATTCTATGTCTACTGTGGGGACTCACGCAACACTCTTATCTTCTGCCAGTGCTGGTGCTGGATACATGGCTTTTGCGCAGCAGAGACGAAGAGAGGTGGAGGAGAAACGATCAAGTGAAAGGAAGTTAGACCGATCGATCTTGAGCCGTTTCTTATATTTCTTCCTAGCAGAGGTAAAGTCATGGAGAGAAGTACAGCCATCATATACAACAGATTTCAAAATTTCAAGAGGAGAAAACGACTCCAGGCAGCACTATTTACGCCTAGATGCACTGCAACAGGCCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HCNGP

Protein Analysis

456

Amino Acids

50.86

Weight (kDa)

4.97

Isoelectric Point (pI)

59.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HCNGP PF07818 187 - 280 1.7e-28 HCNGP-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1155
AccI GTMKAC 3 cut(s) 499, 1006, 1088
AciI CCGC 4 cut(s) 194, 245, 563, 974
AcsI RAATTY 2 cut(s) 146, 1299
AcuI CTGAAG 1 cut(s) 727
AfaI GTAC 3 cut(s) 483, 660, 1272
AfiI CCNNNNNNNGG 6 cut(s) 254, 362, 932, 1000, 1001, 1249
AgsI TTSAA 9 cut(s) 286, 488, 623, 649, 866, 942, 964, 1297, 1305
AjnI CCWGG 5 cut(s) 391, 418, 889, 931, 1322
AluBI AGCT 5 cut(s) 35, 166, 635, 645, 983
AluI AGCT 5 cut(s) 35, 166, 635, 645, 983
Alw26I GTCTC 2 cut(s) 351, 1156
Ama87I CYCGRG 2 cut(s) 175, 996
AoxI GGCC 1 cut(s) 1359
ApeKI GCWGC 7 cut(s) 94, 185, 506, 950, 980, 1156, 1327
ApoI RAATTY 2 cut(s) 146, 1299
Asp700I GAANNNNTTC 1 cut(s) 644
AspLEI GCGC 3 cut(s) 242, 307, 1156
AspS9I GGNCC 1 cut(s) 542
AsuC2I CCSGG 1 cut(s) 256
AsuHPI GGTGA 4 cut(s) 173, 230, 407, 1055
AvaI CYCGRG 2 cut(s) 175, 996
AvaII GGWCC 1 cut(s) 542
AxyI CCTNAGG 2 cut(s) 357, 1066
BbsI GAAGAC 1 cut(s) 81
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 7 cut(s) 106, 172, 493, 937, 992, 1168, 1339
BccI CCATC 4 cut(s) 197, 272, 1034, 1285
BceAI ACGGC 1 cut(s) 1209
BciT130I CCWGG 5 cut(s) 393, 420, 891, 933, 1324
BciVI GTATCC 1 cut(s) 1133
BclI TGATCA 1 cut(s) 724
BcnI CCSGG 1 cut(s) 256
BcoDI GTCTC 2 cut(s) 351, 1156
BfaI CTAG 2 cut(s) 1244, 1343
BfmI CTRYAG 3 cut(s) 676, 978, 1073
BfoI RGCGCY 1 cut(s) 308
BfuI GTATCC 1 cut(s) 1133
BisI GCNGC 7 cut(s) 95, 186, 507, 951, 981, 1157, 1328
BlsI GCNGC 7 cut(s) 96, 187, 508, 952, 982, 1158, 1329
Bme1390I CCNGG 6 cut(s) 256, 393, 420, 891, 933, 1324
Bme18I GGWCC 1 cut(s) 542
BmeT110I CYCGRG 2 cut(s) 175, 996
BmgT120I GGNCC 1 cut(s) 542
BmrFI CCNGG 6 cut(s) 256, 393, 420, 891, 933, 1324
BmrI ACTGGG 1 cut(s) 966
BmsI GCATC 4 cut(s) 14, 16, 172, 1336
BmuI ACTGGG 1 cut(s) 966
BpiI GAAGAC 1 cut(s) 81
BpmI CTGGAG 3 cut(s) 402, 897, 1306
Bpu10I CCTNAGC 2 cut(s) 351, 510
BpuEI CTTGAG 1 cut(s) 1240
BpuMI CCSGG 1 cut(s) 256
Bsa29I ATCGAT 1 cut(s) 1214
BsaAI YACGTR 1 cut(s) 662
BsaBI GATNNNNATC 2 cut(s) 723, 1044
BsaJI CCNNGG 3 cut(s) 255, 945, 995
BsaXI ACNNNNNCTCC 4 cut(s) 331, 361, 400, 430
Bsc4I CCNNNNNNNGG 6 cut(s) 254, 362, 932, 1000, 1001, 1249
Bse118I RCCGGY 1 cut(s) 899
Bse1I ACTGG 2 cut(s) 961, 1125
Bse21I CCTNAGG 2 cut(s) 357, 1066
Bse3DI GCAATG 1 cut(s) 195
Bse8I GATNNNNATC 2 cut(s) 723, 1044
BseBI CCWGG 5 cut(s) 393, 420, 891, 933, 1324
BseCI ATCGAT 1 cut(s) 1214
BseDI CCNNGG 3 cut(s) 255, 945, 995
BseGI GGATG 3 cut(s) 202, 208, 283
BseJI GATNNNNATC 2 cut(s) 723, 1044
BseLI CCNNNNNNNGG 6 cut(s) 254, 362, 932, 1000, 1001, 1249
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 6 cut(s) 158, 342, 348, 483, 501, 1080
BseNI ACTGG 2 cut(s) 961, 1125
BseRI GAGGAG 3 cut(s) 374, 1193, 1323
BseXI GCAGC 7 cut(s) 106, 172, 493, 937, 992, 1168, 1339
BseYI CCCAGC 1 cut(s) 635
Bsh1285I CGRYCG 1 cut(s) 1214
BshFI GGCC 1 cut(s) 1361
BshVI ATCGAT 1 cut(s) 1214
BsiEI CGRYCG 1 cut(s) 1214
BsiHKCI CYCGRG 2 cut(s) 175, 996
BsiSI CCGG 2 cut(s) 255, 900
BslFI GGGAC 1 cut(s) 1111
BslI CCNNNNNNNGG 6 cut(s) 254, 362, 932, 1000, 1001, 1249
BsmAI GTCTC 2 cut(s) 351, 1156
BsmBI CGTCTC 1 cut(s) 1156
BsmFI GGGAC 1 cut(s) 1111
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 1 cut(s) 1361
BsoBI CYCGRG 2 cut(s) 175, 996
Bsp143I GATC 5 cut(s) 724, 1045, 1187, 1211, 1215
Bsp19I CCATGG 1 cut(s) 945
BspACI CCGC 4 cut(s) 194, 245, 563, 974
BspANI GGCC 1 cut(s) 1361
BspCNI CTCAG 6 cut(s) 159, 343, 349, 484, 502, 1079
BspDI ATCGAT 1 cut(s) 1214
BspMAI CTGCAG 2 cut(s) 982, 1077
BspQI GCTCTTC 1 cut(s) 519
BsrDI GCAATG 1 cut(s) 195
BsrFI RCCGGY 1 cut(s) 899
BsrI ACTGG 2 cut(s) 961, 1125
BssAI RCCGGY 1 cut(s) 899
BssECI CCNNGG 3 cut(s) 255, 945, 995
BssMI GATC 5 cut(s) 724, 1045, 1187, 1211, 1215
BssT1I CCWWGG 1 cut(s) 945
Bst2UI CCWGG 5 cut(s) 393, 420, 891, 933, 1324
Bst4CI ACNGT 8 cut(s) 161, 211, 427, 453, 481, 570, 791, 1093
Bst6I CTCTTC 5 cut(s) 292, 519, 845, 1065, 1162
BstBAI YACGTR 1 cut(s) 662
BstC8I GCNNGC 2 cut(s) 702, 978
BstDEI CTNAG 6 cut(s) 167, 351, 357, 492, 510, 1066
BstDSI CCRYGG 1 cut(s) 945
BstENI CCTNNNNNAGG 2 cut(s) 930, 1247
BstF5I GGATG 3 cut(s) 202, 208, 283
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 3 cut(s) 242, 307, 1156
BstKTI GATC 5 cut(s) 727, 1048, 1190, 1214, 1218
BstMAI GTCTC 2 cut(s) 351, 1156
BstMBI GATC 5 cut(s) 724, 1045, 1187, 1211, 1215
BstMCI CGRYCG 1 cut(s) 1214
BstMWI GCNNNNNNNGC 3 cut(s) 191, 512, 905
BstNI CCWGG 5 cut(s) 393, 420, 891, 933, 1324
BstNSI RCATGY 2 cut(s) 506, 704
BstSCI CCNGG 6 cut(s) 254, 391, 418, 889, 931, 1322
BstSFI CTRYAG 3 cut(s) 676, 978, 1073
BstSNI TACGTA 1 cut(s) 662
BstV1I GCAGC 7 cut(s) 106, 172, 493, 937, 992, 1168, 1339
BstV2I GAAGAC 1 cut(s) 81
Bsu15I ATCGAT 1 cut(s) 1214
Bsu36I CCTNAGG 2 cut(s) 357, 1066
BsuI GTATCC 1 cut(s) 1133
BsuRI GGCC 1 cut(s) 1361
BsuTUI ATCGAT 1 cut(s) 1214
BtgI CCRYGG 1 cut(s) 945
BtsCI GGATG 3 cut(s) 202, 208, 283
BtsI GCAGTG 2 cut(s) 711, 1349
BtsIMutI CAGTG 3 cut(s) 711, 1132, 1349
Cac8I GCNNGC 2 cut(s) 702, 978
CfoI GCGC 3 cut(s) 242, 307, 1156
Cfr10I RCCGGY 1 cut(s) 899
Cfr13I GGNCC 1 cut(s) 542
ClaI ATCGAT 1 cut(s) 1214
Csp6I GTAC 3 cut(s) 482, 659, 1271
CviQI GTAC 3 cut(s) 482, 659, 1271
DdeI CTNAG 6 cut(s) 167, 351, 357, 492, 510, 1066
DpnI GATC 5 cut(s) 726, 1047, 1189, 1213, 1217
DpnII GATC 5 cut(s) 724, 1045, 1187, 1211, 1215
Eam1104I CTCTTC 5 cut(s) 292, 519, 845, 1065, 1162
EarI CTCTTC 5 cut(s) 292, 519, 845, 1065, 1162
EciI GGCGGA 1 cut(s) 209
Eco105I TACGTA 1 cut(s) 662
Eco130I CCWWGG 1 cut(s) 945
Eco147I AGGCCT 1 cut(s) 1361
Eco47I GGWCC 1 cut(s) 542
Eco57I CTGAAG 1 cut(s) 727
Eco81I CCTNAGG 2 cut(s) 357, 1066
Eco88I CYCGRG 2 cut(s) 175, 996
EcoNI CCTNNNNNAGG 2 cut(s) 930, 1247
EcoRI GAATTC 1 cut(s) 146
EcoRII CCWGG 5 cut(s) 391, 418, 889, 931, 1322
EcoT14I CCWWGG 1 cut(s) 945
ErhI CCWWGG 1 cut(s) 945
Esp3I CGTCTC 1 cut(s) 1156
FaqI GGGAC 1 cut(s) 1111
FauI CCCGC 1 cut(s) 570
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 3 cut(s) 499, 1006, 1088
Fnu4HI GCNGC 7 cut(s) 95, 186, 507, 951, 981, 1157, 1328
FokI GGATG 3 cut(s) 209, 215, 290
Fsp4HI GCNGC 7 cut(s) 95, 186, 507, 951, 981, 1157, 1328
FspBI CTAG 2 cut(s) 1244, 1343
FspI TGCGCA 1 cut(s) 1155
GlaI GCGC 3 cut(s) 241, 306, 1155
GluI GCNGC 7 cut(s) 95, 186, 507, 951, 981, 1157, 1328
GsaI CCCAGC 1 cut(s) 639
GsuI CTGGAG 3 cut(s) 402, 897, 1306
HaeII RGCGCY 1 cut(s) 308
HaeIII GGCC 1 cut(s) 1361
HapII CCGG 2 cut(s) 255, 900
HhaI GCGC 3 cut(s) 242, 307, 1156
Hin6I GCGC 3 cut(s) 240, 305, 1154
HinP1I GCGC 3 cut(s) 240, 305, 1154
HindIII AAGCTT 1 cut(s) 643
HinfI GANTC 7 cut(s) 221, 488, 682, 853, 1079, 1099, 1319
HpaII CCGG 2 cut(s) 255, 900
HphI GGTGA 4 cut(s) 173, 230, 407, 1055
Hpy166II GTNNAC 4 cut(s) 319, 500, 1007, 1089
Hpy188I TCNGA 7 cut(s) 25, 277, 448, 463, 493, 589, 729
Hpy188III TCNNGA 9 cut(s) 177, 350, 620, 716, 876, 998, 1068, 1219, 1305
Hpy8I GTNNAC 4 cut(s) 319, 500, 1007, 1089
Hpy99I CGWCG 3 cut(s) 62, 135, 155
HpyAV CCTTC 3 cut(s) 110, 368, 664
HpyCH4III ACNGT 8 cut(s) 161, 211, 427, 453, 481, 570, 791, 1093
HpyCH4IV ACGT 2 cut(s) 87, 661
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 512, 905
HpyF3I CTNAG 6 cut(s) 167, 351, 357, 492, 510, 1066
HpySE526I ACGT 2 cut(s) 87, 661
HspAI GCGC 3 cut(s) 240, 305, 1154
Ksp22I TGATCA 1 cut(s) 724
Kzo9I GATC 5 cut(s) 724, 1045, 1187, 1211, 1215
LguI GCTCTTC 1 cut(s) 519
Lsp1109I GCAGC 7 cut(s) 106, 172, 493, 937, 992, 1168, 1339
LweI GCATC 4 cut(s) 14, 16, 172, 1336
MaeI CTAG 2 cut(s) 1244, 1343
MaeII ACGT 2 cut(s) 87, 661
MaeIII GTNAC 2 cut(s) 337, 782
MalI GATC 5 cut(s) 726, 1047, 1189, 1213, 1217
MboI GATC 5 cut(s) 724, 1045, 1187, 1211, 1215
MluCI AATT 5 cut(s) 49, 146, 440, 915, 1299
MlyI GAGTC 4 cut(s) 230, 862, 1093, 1313
MmeI TCCRAC 1 cut(s) 528
MroXI GAANNNNTTC 1 cut(s) 644
MseI TTAA 2 cut(s) 572, 918
MslI CAYNNNNRTG 1 cut(s) 771
MspA1I CMGCKG 1 cut(s) 983
MspI CCGG 2 cut(s) 255, 900
MspR9I CCNGG 6 cut(s) 256, 393, 420, 891, 933, 1324
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 5 cut(s) 393, 420, 891, 933, 1324
MwoI GCNNNNNNNGC 3 cut(s) 191, 512, 905
NciI CCSGG 1 cut(s) 256
NcoI CCATGG 1 cut(s) 945
NdeII GATC 5 cut(s) 724, 1045, 1187, 1211, 1215
NmuCI GTSAC 1 cut(s) 782
NsbI TGCGCA 1 cut(s) 1155
NspI RCATGY 2 cut(s) 506, 704
PaeI GCATGC 1 cut(s) 704
PceI AGGCCT 1 cut(s) 1361
PciSI GCTCTTC 1 cut(s) 519
PctI GAATGC 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 3 cut(s) 488, 682, 1079
PfoI TCCNGGA 1 cut(s) 418
PkrI GCNGC 7 cut(s) 96, 187, 508, 952, 982, 1158, 1329
Ple19I CGATCG 1 cut(s) 1214
PleI GAGTC 4 cut(s) 229, 861, 1093, 1313
PpsI GAGTC 4 cut(s) 229, 861, 1093, 1313
Ppu21I YACGTR 1 cut(s) 662
Psp6I CCWGG 5 cut(s) 391, 418, 889, 931, 1322
PspFI CCCAGC 1 cut(s) 635
PspGI CCWGG 5 cut(s) 391, 418, 889, 931, 1322
PspPI GGNCC 1 cut(s) 542
PstI CTGCAG 2 cut(s) 982, 1077
PvuI CGATCG 1 cut(s) 1214
PvuII CAGCTG 1 cut(s) 983
RsaI GTAC 3 cut(s) 483, 660, 1272
RsaNI GTAC 3 cut(s) 482, 659, 1271
RseI CAYNNNNRTG 1 cut(s) 771
SapI GCTCTTC 1 cut(s) 519
SaqAI TTAA 2 cut(s) 572, 918
SatI GCNGC 7 cut(s) 95, 186, 507, 951, 981, 1157, 1328
Sau3AI GATC 5 cut(s) 724, 1045, 1187, 1211, 1215
Sau96I GGNCC 1 cut(s) 542
SchI GAGTC 4 cut(s) 230, 862, 1093, 1313
ScrFI CCNGG 6 cut(s) 256, 393, 420, 891, 933, 1324
SfaNI GCATC 4 cut(s) 14, 16, 172, 1336
SfcI CTRYAG 3 cut(s) 676, 978, 1073
SinI GGWCC 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 1219
SmoI CTYRAG 1 cut(s) 1219
SnaBI TACGTA 1 cut(s) 662
SphI GCATGC 1 cut(s) 704
Sse9I AATT 5 cut(s) 49, 146, 440, 915, 1299
SseBI AGGCCT 1 cut(s) 1361
SsiI CCGC 4 cut(s) 194, 245, 563, 974
SspMI CTAG 2 cut(s) 1244, 1343
StuI AGGCCT 1 cut(s) 1361
StyD4I CCNGG 6 cut(s) 254, 391, 418, 889, 931, 1322
StyI CCWWGG 1 cut(s) 945
TaaI ACNGT 8 cut(s) 161, 211, 427, 453, 481, 570, 791, 1093
TaiI ACGT 2 cut(s) 90, 664
TaqI TCGA 5 cut(s) 8, 150, 1038, 1048, 1214
TaqII GACCGA 1 cut(s) 1224
TasI AATT 5 cut(s) 49, 146, 440, 915, 1299
TatI WGTACW 2 cut(s) 481, 1270
TfiI GAWTC 3 cut(s) 488, 682, 1079
Tru1I TTAA 2 cut(s) 572, 918
Tru9I TTAA 2 cut(s) 572, 918
TscAI CASTG 3 cut(s) 711, 1132, 1356
TseFI GTSAC 1 cut(s) 782
TseI GCWGC 7 cut(s) 94, 185, 506, 950, 980, 1156, 1327
Tsp45I GTSAC 1 cut(s) 782
TspDTI ATGAA 5 cut(s) 87, 348, 485, 810, 834
TspGWI ACGGA 2 cut(s) 154, 534
TspRI CASTG 3 cut(s) 711, 1132, 1356
VpaK11BI GGWCC 1 cut(s) 542
XagI CCTNNNNNAGG 2 cut(s) 930, 1247
XapI RAATTY 2 cut(s) 146, 1299
XceI RCATGY 2 cut(s) 506, 704
XmiI GTMKAC 3 cut(s) 499, 1006, 1088
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 2 cut(s) 1244, 1343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.