pycom05g28690

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007821
Physical Location & Seq
Forward (+)
29318976 .. 29319754
779 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g28690.3

Sequence Viewer

Length: 639 bp
ATGCATTGCCCCCTAAGCAGCTGCCTCCCACAACAATTGCCACTTATGGCTCCTCCTCCTCCCAAATTCTTCTCTCTCCTCTTCCTCCTCTCTCTCCTCCTCTTCTCTCTCCAAGCTCAAGCCAGAGAGAGCCAATTCTTTAGCAAAGTTGCCAGTTCTAACAACAACAATGTGAAGGAATCAGAGCTCACAAAAACAGAGACTCCAAAAACAGAGTCTCCAGAAACAGAGCTCCTAAAAACAGAGTCCCCAGTTTTCAAGCAAGAGCAAGAGAAAGAGCCAACTTTCATTCCAGAGACTCAAAACGGATACGGCCTCTACGGCCACGAGTCCGGCCAGCTGCCTCCCTCCACCTCATCCAACGGTGGACCCTACACCACAACCAACGGTGCACCCTACAAATACACACCCAACAACAACAACAACCTGCCCTATACGGCCGAGGCAGAGAAGCAGCACAATACAAAGTACCCCGACAGATACAACAACCACTACAATCCCAACAAAAACAACTACTACTACAACGGGAACAACTTTGAGGCCAACCCTAATGGCCTCAGTGACACGAGGTTCGCTCAGAGCAGCTACACCACCACCACTGCCACCCCCAACAACTACCAGAAACAACAACAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

213

Amino Acids

23.92

Weight (kDa)

6.09

Isoelectric Point (pI)

54.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 435
AcoI YGGCCR 3 cut(s) 322, 334, 438
AcsI RAATTY 1 cut(s) 65
AfaI GTAC 1 cut(s) 470
AgsI TTSAA 1 cut(s) 259
AluBI AGCT 6 cut(s) 21, 116, 187, 232, 340, 585
AluI AGCT 6 cut(s) 21, 116, 187, 232, 340, 585
Alw21I GWGCWC 3 cut(s) 189, 234, 394
Alw26I GTCTC 3 cut(s) 194, 222, 290
Alw44I GTGCAC 1 cut(s) 390
AoxI GGCC 6 cut(s) 313, 322, 334, 438, 540, 553
ApaLI GTGCAC 1 cut(s) 390
ApeKI GCWGC 5 cut(s) 18, 21, 340, 454, 582
ApoI RAATTY 1 cut(s) 65
ArsI GACNNNNNNTTYG 4 cut(s) 200, 232, 554, 586
AspS9I GGNCC 1 cut(s) 368
AvaII GGWCC 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 189, 234
BauI CACGAG 2 cut(s) 326, 565
Bbv12I GWGCWC 3 cut(s) 189, 234, 394
BbvI GCAGC 5 cut(s) 8, 30, 327, 466, 594
BceAI ACGGC 3 cut(s) 328, 337, 453
BciVI GTATCC 1 cut(s) 302
BcoDI GTCTC 3 cut(s) 194, 222, 290
BfuAI ACCTGC 1 cut(s) 435
BfuI GTATCC 1 cut(s) 302
BglI GCCNNNNNGGC 1 cut(s) 321
BisI GCNGC 5 cut(s) 19, 22, 341, 455, 583
BlsI GCNGC 5 cut(s) 20, 23, 342, 456, 584
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 368
BmiI GGNNCC 2 cut(s) 51, 370
BmrI ACTGGG 1 cut(s) 245
BmuI ACTGGG 1 cut(s) 245
BplI GAGNNNNNCTC 2 cut(s) 559, 591
BpmI CTGGAG 1 cut(s) 204
Bpu10I CCTNAGC 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 441
BsaXI ACNNNNNCTCC 4 cut(s) 187, 202, 217, 232
Bse1I ACTGG 2 cut(s) 153, 251
Bse3DI GCAATG 1 cut(s) 4
BseDI CCNNGG 1 cut(s) 441
BseGI GGATG 1 cut(s) 356
BseMI GCAATG 1 cut(s) 4
BseMII CTCAG 2 cut(s) 571, 590
BseNI ACTGG 2 cut(s) 153, 251
BseRI GAGGAG 7 cut(s) 42, 45, 48, 68, 77, 86, 89
BseSI GKGCMC 1 cut(s) 394
BseX3I CGGCCG 1 cut(s) 438
BseXI GCAGC 5 cut(s) 8, 30, 327, 466, 594
Bsh1285I CGRYCG 1 cut(s) 441
BshFI GGCC 6 cut(s) 315, 324, 336, 440, 542, 555
BsiEI CGRYCG 1 cut(s) 441
BsiHKAI GWGCWC 3 cut(s) 189, 234, 394
BsiSI CCGG 1 cut(s) 333
BslFI GGGAC 1 cut(s) 232
BsmAI GTCTC 3 cut(s) 194, 222, 290
BsmFI GGGAC 1 cut(s) 232
BsnI GGCC 6 cut(s) 315, 324, 336, 440, 542, 555
Bsp1286I GDGCHC 3 cut(s) 189, 234, 394
BspANI GGCC 6 cut(s) 315, 324, 336, 440, 542, 555
BspCNI CTCAG 2 cut(s) 570, 589
BspLI GGNNCC 2 cut(s) 51, 370
BspMI ACCTGC 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 4
BsrI ACTGG 2 cut(s) 153, 251
BssECI CCNNGG 1 cut(s) 441
BssSI CACGAG 2 cut(s) 326, 565
Bst2BI CACGAG 2 cut(s) 326, 565
Bst4CI ACNGT 2 cut(s) 365, 389
Bst6I CTCTTC 2 cut(s) 86, 107
BstC8I GCNNGC 1 cut(s) 338
BstDEI CTNAG 3 cut(s) 14, 557, 576
BstF5I GGATG 1 cut(s) 356
BstMAI GTCTC 3 cut(s) 194, 222, 290
BstMCI CGRYCG 1 cut(s) 441
BstMWI GCNNNNNNNGC 2 cut(s) 15, 321
BstSLI GKGCMC 1 cut(s) 394
BstV1I GCAGC 5 cut(s) 8, 30, 327, 466, 594
BstZI CGGCCG 1 cut(s) 438
BsuI GTATCC 1 cut(s) 302
BsuRI GGCC 6 cut(s) 315, 324, 336, 440, 542, 555
BtsCI GGATG 1 cut(s) 356
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 2 cut(s) 565, 597
BveI ACCTGC 1 cut(s) 435
Cac8I GCNNGC 1 cut(s) 338
Cfr13I GGNCC 1 cut(s) 368
Csp6I GTAC 1 cut(s) 469
CspCI CAANNNNNGTGG 2 cut(s) 18, 53
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 3 cut(s) 14, 557, 576
EaeI YGGCCR 3 cut(s) 322, 334, 438
EagI CGGCCG 1 cut(s) 438
Eam1104I CTCTTC 2 cut(s) 86, 107
EarI CTCTTC 2 cut(s) 86, 107
Ecl136II GAGCTC 2 cut(s) 187, 232
EclXI CGGCCG 1 cut(s) 438
Eco24I GRGCYC 2 cut(s) 189, 234
Eco47I GGWCC 1 cut(s) 368
Eco52I CGGCCG 1 cut(s) 438
Eco53kI GAGCTC 2 cut(s) 187, 232
EcoICRI GAGCTC 2 cut(s) 187, 232
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 189, 234
FaiI YATR 2 cut(s) 47, 435
FaqI GGGAC 1 cut(s) 232
Fnu4HI GCNGC 5 cut(s) 19, 22, 341, 455, 583
FokI GGATG 1 cut(s) 343
FriOI GRGCYC 2 cut(s) 189, 234
Fsp4HI GCNGC 5 cut(s) 19, 22, 341, 455, 583
GluI GCNGC 5 cut(s) 19, 22, 341, 455, 583
GsuI CTGGAG 1 cut(s) 204
HaeIII GGCC 6 cut(s) 315, 324, 336, 440, 542, 555
HapII CCGG 1 cut(s) 333
HinfI GANTC 6 cut(s) 179, 202, 215, 245, 298, 329
HpaII CCGG 1 cut(s) 333
Hpy166II GTNNAC 2 cut(s) 368, 392
Hpy188I TCNGA 2 cut(s) 184, 579
Hpy188III TCNNGA 2 cut(s) 221, 293
Hpy8I GTNNAC 2 cut(s) 368, 392
HpyAV CCTTC 1 cut(s) 169
HpyCH4III ACNGT 2 cut(s) 365, 389
HpyCH4V TGCA 2 cut(s) 4, 392
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 321
HpyF3I CTNAG 3 cut(s) 14, 557, 576
LmnI GCTCC 2 cut(s) 55, 237
LpnPI CCDG 9 cut(s) 136, 166, 234, 264, 306, 346, 350, 440, 632
Lsp1109I GCAGC 5 cut(s) 8, 30, 327, 466, 594
MaeIII GTNAC 1 cut(s) 560
MboII GAAGA 3 cut(s) 61, 73, 94
MfeI CAATTG 1 cut(s) 35
MhlI GDGCHC 3 cut(s) 189, 234, 394
MluCI AATT 3 cut(s) 35, 65, 134
MlyI GAGTC 5 cut(s) 196, 224, 254, 292, 338
MmeI TCCRAC 1 cut(s) 384
Mph1103I ATGCAT 1 cut(s) 6
MspA1I CMGCKG 2 cut(s) 21, 340
MspI CCGG 1 cut(s) 333
MunI CAATTG 1 cut(s) 35
MwoI GCNNNNNNNGC 2 cut(s) 15, 321
NlaIV GGNNCC 2 cut(s) 51, 370
NmeAIII GCCGAG 1 cut(s) 466
NmuCI GTSAC 1 cut(s) 560
NsiI ATGCAT 1 cut(s) 6
PfeI GAWTC 1 cut(s) 179
PkrI GCNGC 5 cut(s) 20, 23, 342, 456, 584
PleI GAGTC 5 cut(s) 196, 223, 253, 292, 337
PpsI GAGTC 5 cut(s) 196, 223, 253, 292, 337
Psp124BI GAGCTC 2 cut(s) 189, 234
PspN4I GGNNCC 2 cut(s) 51, 370
PspPI GGNCC 1 cut(s) 368
PvuII CAGCTG 2 cut(s) 21, 340
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
SacI GAGCTC 2 cut(s) 189, 234
SatI GCNGC 5 cut(s) 19, 22, 341, 455, 583
Sau96I GGNCC 1 cut(s) 368
SchI GAGTC 5 cut(s) 196, 224, 254, 292, 338
SduI GDGCHC 3 cut(s) 189, 234, 394
SetI ASST 9 cut(s) 23, 118, 189, 234, 342, 356, 429, 572, 587
SfiI GGCCNNNNNGGCC 1 cut(s) 321
SinI GGWCC 1 cut(s) 368
SmlI CTYRAG 1 cut(s) 117
SmoI CTYRAG 1 cut(s) 117
Sse9I AATT 3 cut(s) 35, 65, 134
SstI GAGCTC 2 cut(s) 189, 234
TaaI ACNGT 2 cut(s) 365, 389
TasI AATT 3 cut(s) 35, 65, 134
TfiI GAWTC 1 cut(s) 179
TscAI CASTG 2 cut(s) 565, 604
TseFI GTSAC 1 cut(s) 560
TseI GCWGC 5 cut(s) 18, 21, 340, 454, 582
Tsp45I GTSAC 1 cut(s) 560
TspDTI ATGAA 1 cut(s) 277
TspGWI ACGGA 1 cut(s) 321
TspRI CASTG 2 cut(s) 565, 604
VneI GTGCAC 1 cut(s) 390
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 1 cut(s) 65
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.