Rorug04G0441200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007821
Physical Location & Seq
Forward (+)
59789356 .. 59800541
11186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0441200.1

Sequence Viewer

Length: 3666 bp
ATGAGCGGATGGAATCGGTCGTCGAGATCGAGGCTCGGAGGTCGCAGTAACCCGCCGCTCGAGCGAACCACGACGACGACGACGGTGCGGCTCGGACGAGTCCAGCCGCAAGCGCCGAGTAACCGCACCATTTACTGCAATGATCGCGAGGCTAATCTTCCCGTCAAATTTGTGGGGAATTCTATAGCAACTACAAAGTACAACTTCTTAACTTTTTTGCCGAAAGGACTCTTTGAACAGTTCAGACGGGTAGCTAACCTTTACTTCCTTGGAATCTCAATTTTATCAACAACACCAATCAGTCCCGTGAATCCAGTAACCAACGTGGTACCGCTGAGTTTTGTGCTTTTTCTTTCTCTTGTAAAGGAAGCATGGGAGGATTGGAAGCGTTTCCAGAATGATATGACAATAAATAATAATTCAGTGGATGTCTTGCAAGATCAGAGGTGGGAAACTATTCCTTGGAAAAGGTTACAAGTTGGAGATATCGTCAGGATTAAGCAAAACGCATTCTTCCCTGCGGATCTTCTTTTTCTTGCAACTACAAACGCAGATGGTGTCTGCTACATTGAGACTGCAAATTTGGATGGGGAAACTAATTTAAAAATCAGAAAAGCACTGGAAAAGACATGGGATTACTTGACTCCTGAGAAAGCATCTGAATTTAAAGGAGAACTGCAGTGTGAACAACCAAACAATTCATTGTACACTTTCACCGGCAATCTCATTATTGACAAGCAGACACTACCTCTCTCTCCTAACCATCTTCTTCTCCGTGGATGCAGCCTAAGGAACACGGAATACATAGTTGGGGCTGTTGTATTTACCGGTCATGAAACCAAGGTTATGATGAATAGCATGAATGTTCCTTCTAAAAGAAGTACATTAGAGAGGAAACTTGACAAACTTATCATTGGGCTTTTTATTACTCTCTTTTGTATGTGTCTGATTGGAGCCATATGCAGTGGTGTATTTATCAACTACAAGTACTACTACTTAGGTCTGCGGGGATCAAAGGGAGAGAACATATCGTACAGCTCATTTAACCCTGATAATCGTTTTCTGGTCATCATTCTGAATATGTTTACCTTGATTACTCTATACTCAACAATCATCCCTATTTCTCTTTACATTTCCATTGAGATGATCAAATTTATTCAGTCCACCCAATATATCAACAATGACTTGCGTATGTACCACATGGAAAGCAACACCCCTGCATTGGCTAGGACTTCGAATTTGAATGAGGAACTTGGACAGGTGGAATACATCTTTTCTGATAAAACTGGAACTTTAACAAGAAACTTGATGGAGTTCTTCAAGTGTTCAATTGGAGGAGAGGTCTATGGAACTGGTGTCACTGAAATAGAAAGGGGAATAGCACAAAGAAGTGGTATAAAGCTTAATGAGGAGTACAAGTCTGACACAGTGCATGACAAGGGATTTAACTTCGACGATGCCAAGCTCATGCGGGGAGCTTGGAGGAATGAACCTAATCCAGATATCTGTAAGGAATTTTTCAGATGCCTTGCTATTTGCCATACTGTACTTCCAGAGGGTGATGAGTCCCCTGAGAAGATCACATATCAAGCTGCATCCCCTGATGAGTCTGCTTTGGTCATTGCTGCGAAGAATTTTGGTTTCTTCTTTTACAGGCGTTCACCGACTACTATATGTGTTAGAGAATCTCATGTTGAGAAAGTTGGTGATGTCCAAGATGTGTCTTACGAGATTCTAAATGTCCTCGAGTTTAACAGTACAAGGAAGCGTCAGTCTGTTGTATGCCGTTATCCAGATGGCAGGCTTGTCTTGTACTGCAAGGGTGCTGATAATGTAATCTATGAGAGACTGGCTGATGGCCAAGATGATCTGAAGAAAGTATCAAGGAAACACTTGGAACTATTTGGGTCTTCTGGATTACGTACTCTTTGCCTGGCCTATAGAGATTTAAGTCCTGATGTGTATGAAAGCTGGAATGAGAAATTTATTCAGGCTAAATCCTCTCTACGGGATCGTGAAAAGAAGCTGGATGAGGTGGCAGAACTCATTGAGATTGATCTCATATTGATTGGATGCACTGCTATTGAAGACAAGCTTCAGGAAGGAGTTCCAGCTTGTATAGAGACTCTTGCTAGAGCTGGTATCAAGATTTGGATGCTAACAGGAGACAAGATGGAAACAGCTATAAATATAGGTTATGCATGCAATTTGATCAATAATGAAATGAAACAGTTCATTATCAGCTCAGAAACTGATGTTATTCGGGAAGCTGAGAACAGGGGTGACCAAGTGGAAATTGCTCGTGTCATTAAAGATGAAGTGAAGAAAGATCTGAAGAGGTGCCTCGAGGAAGCACAGCACTATCTTCGCACTGTGTCTGGGCCAAAATTAGCACTTGTAATAGATGGCAAGTGTTTGATGTATGCCTTGGAACCAACTTTACGAGTGATGCTACTGAACTTGAGCTTGAACTGTCATTCAGTTGTGTGCTGTCGAGTTTCTCCTTTACAGAAAGCACAGGTGACAAGTATGGTAAGAAAAGGTGCCCGGAAAATAACACTTAGCATTGGTGATGGTGCCAATGATGTGAGCATGATCCAAGCTGCTCATGTTGGCGTTGGAATAAGTGGGCAAGAGGGGATGCAGGCAGTGATGGCTAGTGATTTTGCTATTGCCCAGTTCCGCTTTCTTACTGATTTACTTCTTGTCCATGGACGATGGTCGTATATCAGATTATGCAAGGTCATCACATACTTCTTTTACAAGAATCTTACATTCACTTTGACACAATTTTGGTTCACCTTTTATACTGGATATTCTGGTCAAAGGTTCTATGATGATTGGTATCAATCACTATATAATGTCATATTCACAGCCTTGCCCGTAGTCATTGTCGGGCTTTTTGACAAGGATGTCAGTGCAGCCCTTTCCAAAAAGTACCCTGAACTATACAGGGAGGGAATAAGAAATGTGTTCTTCAGATGGAGGGTTGTGGCAACGTGGGCCTTCTTTTCTGTTTACCAATCTTTAATATTCTTCTATGTTGTGACTAGCTCTGCTGTTAGAAGTGTGGATTCATCTGGCAAGATGTTTGGCCATATGGATGTCAGCACAATGGCTTTCACTTGTGTTGTAGTAACTGTCAACTTGCGTCTGCTTATGACCTGTAATTCAATCACAAGGTGGCATTATATTAGTGTTGGAGGAAGCATTTTAGCGTGGTTTATATTCATTTTCATATACTGTTTTGCAGACCATAGGAAAAGTGTTTATTATGTCATATTTGTTTTGATGAGTACATTCTACTTCTACATGACACTTCTTCTTGTGCCCATTCTTGCGCTCTTTGGCGACTTTGTTTACCAAGTGTTGCAAAGATGGTTCTTCCCATATGATTATCAGATAATTCAGGAACTTCACAGGGATGAGCCAGAAGGTAGGCGCAGGGAAGATTTGCTGGAAATTGGAAACAACCTCACCCCTGCCGAGGCGAGGAGCTATGCTGTAGCTCAACTCCCACGGGAGATTTCAAAACACACTGGCTTTGCTTTTGACTCACCTGGATATGAATCATTCTTCGCTAAACAGGCAGGTGTATATGCCCCTCAAAAAGCATGGGATGTTGCTCGGAGAGCCAGCATGAGAAGGACAGCGAAAAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1221

Amino Acids

139.5

Weight (kDa)

8.59

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhoLip_ATPase_N PF16209 44 - 108 7.2e-25 Phospholipid-translocating ATPase N-terminal
Cation_ATPase PF13246 503 - 701 3.8e-20 P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C PF16212 884 - 1130 1.8e-82 Phospholipid-translocating P-type ATPase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3581
AasI GACNNNNNNGTC 1 cut(s) 2912
AbsI CCTCGAGG 1 cut(s) 2345
Acc36I ACCTGC 1 cut(s) 3581
Acc65I GGTACC 1 cut(s) 328
AccB1I GGYRCC 4 cut(s) 328, 2340, 2543, 2576
AccBSI CCGCTC 2 cut(s) 6, 58
AccII CGCG 1 cut(s) 147
AclWI GGATC 4 cut(s) 531, 1018, 2019, 2590
AcoI YGGCCR 2 cut(s) 1858, 3094
AcsI RAATTY 9 cut(s) 167, 178, 580, 662, 1151, 1237, 1514, 1633, 1982
AcuI CTGAAG 4 cut(s) 1892, 2081, 2354, 2962
AdeI CACNNNGTG 1 cut(s) 3183
AfiI CCNNNNNNNGG 4 cut(s) 1222, 2007, 3487, 3492
AgeI ACCGGT 1 cut(s) 827
AgsI TTSAA 8 cut(s) 236, 1243, 1321, 1329, 2087, 2470, 3174, 3531
AjnI CCWGG 2 cut(s) 1932, 3559
AjuI GAANNNNNNNTTGG 2 cut(s) 792, 824
Alw26I GTCTC 4 cut(s) 566, 1840, 2117, 2160
AlwI GGATC 4 cut(s) 531, 1018, 2019, 2590
AlwNI CAGNNNCTG 1 cut(s) 2252
Ama87I CYCGRG 3 cut(s) 59, 1745, 2345
AoxI GGCC 5 cut(s) 1858, 1935, 2381, 3003, 3094
ApeKI GCWGC 5 cut(s) 783, 1592, 1625, 2603, 2921
ApoI RAATTY 9 cut(s) 167, 178, 580, 662, 1151, 1237, 1514, 1633, 1982
ArsI GACNNNNNNTTYG 4 cut(s) 565, 597, 1222, 1254
AsiGI ACCGGT 1 cut(s) 827
Asp700I GAANNNNTTC 4 cut(s) 389, 456, 2106, 2231
Asp718I GGTACC 1 cut(s) 328
AspLEI GCGC 3 cut(s) 115, 3343, 3444
AspS9I GGNCC 2 cut(s) 2381, 3003
AsuC2I CCSGG 1 cut(s) 2548
AsuII TTCGAA 1 cut(s) 1235
AvaI CYCGRG 3 cut(s) 59, 1745, 2345
AxyI CCTNAGG 1 cut(s) 788
BaeGI GKGCMC 2 cut(s) 2548, 3333
BalI TGGCCA 2 cut(s) 1860, 3096
BanI GGYRCC 4 cut(s) 328, 2340, 2543, 2576
BauI CACGAG 1 cut(s) 2301
BbsI GAAGAC 2 cut(s) 1902, 2094
BbvI GCAGC 5 cut(s) 795, 1579, 1612, 2590, 2933
BceAI ACGGC 1 cut(s) 1770
BcgI CGANNNNNNTGC 4 cut(s) 67, 101, 2348, 2382
BciT130I CCWGG 2 cut(s) 1934, 3561
BclI TGATCA 2 cut(s) 1146, 2211
BcnI CCSGG 1 cut(s) 2548
BcoDI GTCTC 4 cut(s) 566, 1840, 2117, 2160
BfaI CTAG 4 cut(s) 1227, 2133, 2658, 3051
BfmI CTRYAG 4 cut(s) 183, 677, 1939, 3504
BfoI RGCGCY 1 cut(s) 116
BfuAI ACCTGC 1 cut(s) 3581
BglII AGATCT 1 cut(s) 2329
BisI GCNGC 8 cut(s) 56, 89, 107, 784, 1593, 1626, 2604, 2922
BlsI GCNGC 8 cut(s) 57, 90, 108, 785, 1594, 1627, 2605, 2923
BmcAI AGTACT 1 cut(s) 989
Bme1390I CCNGG 3 cut(s) 1934, 2548, 3561
BmeT110I CYCGRG 3 cut(s) 59, 1745, 2345
BmgT120I GGNCC 2 cut(s) 2381, 3003
BmiI GGNNCC 6 cut(s) 330, 955, 2342, 2433, 2545, 2578
BmrFI CCNGG 3 cut(s) 1934, 2548, 3561
BmrI ACTGGG 1 cut(s) 2671
BmsI GCATC 9 cut(s) 665, 770, 1447, 1514, 1604, 2063, 2145, 2439, 2631
BmuI ACTGGG 1 cut(s) 2671
BoxI GACNNNNGTC 1 cut(s) 2719
BpiI GAAGAC 2 cut(s) 1902, 2094
Bpu14I TTCGAA 1 cut(s) 1235
BpuEI CTTGAG 1 cut(s) 2482
BpuMI CCSGG 1 cut(s) 2548
BsaAI YACGTR 1 cut(s) 1922
BsaBI GATNNNNATC 2 cut(s) 2844, 3568
BsaJI CCNNGG 8 cut(s) 268, 461, 775, 840, 2427, 2710, 3486, 3518
BsaWI WCCGGW 1 cut(s) 827
BsaXI ACNNNNNCTCC 6 cut(s) 474, 504, 1326, 1356, 1403, 1433
Bsc4I CCNNNNNNNGG 4 cut(s) 1222, 2007, 3487, 3492
Bse118I RCCGGY 2 cut(s) 716, 827
Bse1I ACTGG 8 cut(s) 314, 624, 1291, 1357, 1854, 2677, 2815, 3544
Bse21I CCTNAGG 1 cut(s) 788
Bse3DI GCAATG 2 cut(s) 145, 1620
Bse8I GATNNNNATC 2 cut(s) 2844, 3568
BseBI CCWGG 2 cut(s) 1934, 3561
BseDI CCNNGG 8 cut(s) 268, 461, 775, 840, 2427, 2710, 3486, 3518
BseJI GATNNNNATC 2 cut(s) 2844, 3568
BseLI CCNNNNNNNGG 4 cut(s) 1222, 2007, 3487, 3492
BseMI GCAATG 2 cut(s) 145, 1620
BseMII CTCAG 5 cut(s) 326, 639, 1563, 2259, 2262
BseNI ACTGG 8 cut(s) 314, 624, 1291, 1357, 1854, 2677, 2815, 3544
BseRI GAGGAG 3 cut(s) 1350, 1424, 3508
BseSI GKGCMC 2 cut(s) 2548, 3333
BseXI GCAGC 5 cut(s) 795, 1579, 1612, 2590, 2933
BsgI GTGCAG 1 cut(s) 2940
Bsh1236I CGCG 1 cut(s) 147
Bsh1285I CGRYCG 1 cut(s) 20
BshFI GGCC 5 cut(s) 1860, 1937, 2383, 3005, 3096
BshNI GGYRCC 4 cut(s) 328, 2340, 2543, 2576
BshTI ACCGGT 1 cut(s) 827
BsiEI CGRYCG 1 cut(s) 20
BsiHKCI CYCGRG 3 cut(s) 59, 1745, 2345
BsiSI CCGG 3 cut(s) 717, 828, 2548
BslFI GGGAC 2 cut(s) 288, 1552
BslI CCNNNNNNNGG 4 cut(s) 1222, 2007, 3487, 3492
BsmAI GTCTC 4 cut(s) 566, 1840, 2117, 2160
BsmFI GGGAC 2 cut(s) 288, 1552
BsmI GAATGC 1 cut(s) 509
BsnI GGCC 5 cut(s) 1860, 1937, 2383, 3005, 3096
BsoBI CYCGRG 3 cut(s) 59, 1745, 2345
Bsp119I TTCGAA 1 cut(s) 1235
Bsp1286I GDGCHC 2 cut(s) 2548, 3333
Bsp1407I TGTACA 1 cut(s) 705
Bsp19I CCATGG 1 cut(s) 2710
Bsp68I TCGCGA 1 cut(s) 147
BspANI GGCC 5 cut(s) 1860, 1937, 2383, 3005, 3096
BspCNI CTCAG 5 cut(s) 327, 640, 1564, 2258, 2263
BspFNI CGCG 1 cut(s) 147
BspHI TCATGA 1 cut(s) 832
BspLI GGNNCC 6 cut(s) 330, 955, 2342, 2433, 2545, 2578
BspMAI CTGCAG 1 cut(s) 681
BspMI ACCTGC 1 cut(s) 3581
BspPI GGATC 4 cut(s) 531, 1018, 2019, 2590
BspT104I TTCGAA 1 cut(s) 1235
BspT107I GGYRCC 4 cut(s) 328, 2340, 2543, 2576
BsrBI CCGCTC 2 cut(s) 6, 58
BsrDI GCAATG 2 cut(s) 145, 1620
BsrFI RCCGGY 2 cut(s) 716, 827
BsrGI TGTACA 1 cut(s) 705
BsrI ACTGG 8 cut(s) 314, 624, 1291, 1357, 1854, 2677, 2815, 3544
BssAI RCCGGY 2 cut(s) 716, 827
BssECI CCNNGG 8 cut(s) 268, 461, 775, 840, 2427, 2710, 3486, 3518
BssSI CACGAG 1 cut(s) 2301
BssT1I CCWWGG 5 cut(s) 268, 461, 840, 2427, 2710
Bst2BI CACGAG 1 cut(s) 2301
Bst2UI CCWGG 2 cut(s) 1934, 3561
Bst6I CTCTTC 1 cut(s) 2330
BstAUI TGTACA 1 cut(s) 705
BstBAI YACGTR 1 cut(s) 1922
BstBI TTCGAA 1 cut(s) 1235
BstC8I GCNNGC 5 cut(s) 111, 1802, 2203, 2646, 3637
BstDEI CTNAG 8 cut(s) 335, 648, 788, 997, 1572, 2245, 2271, 2561
BstDSI CCRYGG 3 cut(s) 775, 2710, 3518
BstEII GGTNACC 1 cut(s) 2282
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 116
BstHHI GCGC 3 cut(s) 115, 3343, 3444
BstMAI GTCTC 4 cut(s) 566, 1840, 2117, 2160
BstMCI CGRYCG 1 cut(s) 20
BstMWI GCNNNNNNNGC 7 cut(s) 61, 112, 144, 2654, 3002, 3587, 3632
BstNI CCWGG 2 cut(s) 1934, 3561
BstNSI RCATGY 1 cut(s) 2205
BstPAI GACNNNNGTC 1 cut(s) 2719
BstPI GGTNACC 1 cut(s) 2282
BstSCI CCNGG 3 cut(s) 1932, 2546, 3559
BstSFI CTRYAG 4 cut(s) 183, 677, 1939, 3504
BstSLI GKGCMC 2 cut(s) 2548, 3333
BstSNI TACGTA 1 cut(s) 1922
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 5 cut(s) 795, 1579, 1612, 2590, 2933
BstV2I GAAGAC 2 cut(s) 1902, 2094
BstX2I RGATCY 2 cut(s) 523, 2329
BstYI RGATCY 2 cut(s) 523, 2329
Bsu36I CCTNAGG 1 cut(s) 788
BsuRI GGCC 5 cut(s) 1860, 1937, 2383, 3005, 3096
BtgI CCRYGG 3 cut(s) 775, 2710, 3518
BtsI GCAGTG 4 cut(s) 686, 970, 2076, 2655
BtuMI TCGCGA 1 cut(s) 147
BveI ACCTGC 1 cut(s) 3581
Cac8I GCNNGC 5 cut(s) 111, 1802, 2203, 2646, 3637
CaiI CAGNNNCTG 1 cut(s) 2252
CciI TCATGA 1 cut(s) 832
CfoI GCGC 3 cut(s) 115, 3343, 3444
Cfr10I RCCGGY 2 cut(s) 716, 827
Cfr13I GGNCC 2 cut(s) 2381, 3003
CseI GACGC 2 cut(s) 1757, 3140
CspAI ACCGGT 1 cut(s) 827
DdeI CTNAG 8 cut(s) 335, 648, 788, 997, 1572, 2245, 2271, 2561
DraI TTTAAA 2 cut(s) 603, 667
DraIII CACNNNGTG 1 cut(s) 3183
DrdI GACNNNNNNGTC 1 cut(s) 2912
DseDI GACNNNNNNGTC 1 cut(s) 2912
EaeI YGGCCR 2 cut(s) 1858, 3094
Eam1104I CTCTTC 1 cut(s) 2330
EarI CTCTTC 1 cut(s) 2330
Eco105I TACGTA 1 cut(s) 1922
Eco130I CCWWGG 5 cut(s) 268, 461, 840, 2427, 2710
Eco32I GATATC 2 cut(s) 487, 1504
Eco57I CTGAAG 4 cut(s) 1892, 2081, 2354, 2962
Eco81I CCTNAGG 1 cut(s) 788
Eco88I CYCGRG 3 cut(s) 59, 1745, 2345
Eco91I GGTNACC 1 cut(s) 2282
EcoO65I GGTNACC 1 cut(s) 2282
EcoRI GAATTC 1 cut(s) 178
EcoRII CCWGG 2 cut(s) 1932, 3559
EcoRV GATATC 2 cut(s) 487, 1504
EcoT14I CCWWGG 5 cut(s) 268, 461, 840, 2427, 2710
EcoT22I ATGCAT 1 cut(s) 2203
ErhI CCWWGG 5 cut(s) 268, 461, 840, 2427, 2710
FalI AAGNNNNNCTT 4 cut(s) 188, 220, 2079, 2111
FaqI GGGAC 2 cut(s) 288, 1552
FauI CCCGC 3 cut(s) 60, 999, 1464
FauNDI CATATG 3 cut(s) 959, 3099, 3391
FbaI TGATCA 2 cut(s) 1146, 2211
Fnu4HI GCNGC 8 cut(s) 56, 89, 107, 784, 1593, 1626, 2604, 2922
Fsp4HI GCNGC 8 cut(s) 56, 89, 107, 784, 1593, 1626, 2604, 2922
FspBI CTAG 4 cut(s) 1227, 2133, 2658, 3051
GlaI GCGC 3 cut(s) 114, 3342, 3443
GluI GCNGC 8 cut(s) 56, 89, 107, 784, 1593, 1626, 2604, 2922
HaeII RGCGCY 1 cut(s) 116
HaeIII GGCC 5 cut(s) 1860, 1937, 2383, 3005, 3096
HapII CCGG 3 cut(s) 717, 828, 2548
HgaI GACGC 2 cut(s) 1757, 3140
HhaI GCGC 3 cut(s) 115, 3343, 3444
Hin6I GCGC 3 cut(s) 113, 3341, 3442
HinP1I GCGC 3 cut(s) 113, 3341, 3442
HincII GTYRAC 1 cut(s) 3145
HindII GTYRAC 1 cut(s) 3145
HindIII AAGCTT 2 cut(s) 1400, 2093
HpaII CCGG 3 cut(s) 717, 828, 2548
Hpy166II GTNNAC 9 cut(s) 686, 708, 1086, 1164, 1661, 2799, 3019, 3145, 3361
Hpy8I GTNNAC 9 cut(s) 686, 708, 1086, 1164, 1661, 2799, 3019, 3145, 3361
Hpy99I CGWCG 6 cut(s) 25, 76, 79, 82, 85, 1457
HpyAV CCTTC 5 cut(s) 879, 2096, 3016, 3428, 3639
HpyCH4IV ACGT 3 cut(s) 324, 1921, 2999
HpyF10VI GCNNNNNNNGC 7 cut(s) 61, 112, 144, 2654, 3002, 3587, 3632
HpyF3I CTNAG 8 cut(s) 335, 648, 788, 997, 1572, 2245, 2271, 2561
HpySE526I ACGT 3 cut(s) 324, 1921, 2999
HspAI GCGC 3 cut(s) 113, 3341, 3442
KpnI GGTACC 1 cut(s) 332
Ksp22I TGATCA 2 cut(s) 1146, 2211
LmnI GCTCC 3 cut(s) 953, 1475, 3495
Lsp1109I GCAGC 5 cut(s) 795, 1579, 1612, 2590, 2933
LweI GCATC 9 cut(s) 665, 770, 1447, 1514, 1604, 2063, 2145, 2439, 2631
MaeI CTAG 4 cut(s) 1227, 2133, 2658, 3051
MaeII ACGT 3 cut(s) 324, 1921, 2999
MaeIII GTNAC 9 cut(s) 47, 119, 316, 471, 1357, 2282, 2521, 3046, 3136
MbiI CCGCTC 2 cut(s) 6, 58
MfeI CAATTG 1 cut(s) 1329
MflI RGATCY 2 cut(s) 523, 2329
MhlI GDGCHC 2 cut(s) 2548, 3333
MlsI TGGCCA 2 cut(s) 1860, 3096
MluNI TGGCCA 2 cut(s) 1860, 3096
MlyI GAGTC 7 cut(s) 108, 222, 637, 1574, 1616, 2119, 3548
MmeI TCCRAC 3 cut(s) 460, 2599, 3181
Mox20I TGGCCA 2 cut(s) 1860, 3096
Mph1103I ATGCAT 1 cut(s) 2203
MroXI GAANNNNTTC 4 cut(s) 389, 456, 2106, 2231
MscI TGGCCA 2 cut(s) 1860, 3096
MslI CAYNNNNRTG 3 cut(s) 1142, 3102, 3423
Msp20I TGGCCA 2 cut(s) 1860, 3096
MspA1I CMGCKG 1 cut(s) 334
MspI CCGG 3 cut(s) 717, 828, 2548
MspR9I CCNGG 3 cut(s) 1934, 2548, 3561
MunI CAATTG 1 cut(s) 1329
Mva1269I GAATGC 1 cut(s) 509
MvaI CCWGG 2 cut(s) 1934, 3561
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 7 cut(s) 61, 112, 144, 2654, 3002, 3587, 3632
NciI CCSGG 1 cut(s) 2548
NcoI CCATGG 1 cut(s) 2710
NdeI CATATG 3 cut(s) 959, 3099, 3391
NlaIV GGNNCC 6 cut(s) 330, 955, 2342, 2433, 2545, 2578
NmeAIII GCCGAG 2 cut(s) 141, 3511
NmuCI GTSAC 4 cut(s) 1357, 2282, 2521, 3046
NruI TCGCGA 1 cut(s) 147
NsiI ATGCAT 1 cut(s) 2203
NspI RCATGY 1 cut(s) 2205
NspV TTCGAA 1 cut(s) 1235
PaeI GCATGC 1 cut(s) 2205
PaeR7I CTCGAG 3 cut(s) 59, 1745, 2345
PagI TCATGA 1 cut(s) 832
PaqCI CACCTGC 1 cut(s) 3581
PcsI WCGNNNNNNNCGW 2 cut(s) 26, 77
PctI GAATGC 1 cut(s) 509
PdmI GAANNNNTTC 4 cut(s) 389, 456, 2106, 2231
PfeI GAWTC 8 cut(s) 13, 273, 310, 1685, 1732, 2767, 3074, 3569
PinAI ACCGGT 1 cut(s) 827
PkrI GCNGC 8 cut(s) 57, 90, 108, 785, 1594, 1627, 2605, 2923
PleI GAGTC 7 cut(s) 107, 222, 637, 1573, 1615, 2119, 3548
PpsI GAGTC 7 cut(s) 107, 222, 637, 1573, 1615, 2119, 3548
Ppu21I YACGTR 1 cut(s) 1922
PshAI GACNNNNGTC 1 cut(s) 2719
Psp6I CCWGG 2 cut(s) 1932, 3559
PspEI GGTNACC 1 cut(s) 2282
PspGI CCWGG 2 cut(s) 1932, 3559
PspN4I GGNNCC 6 cut(s) 330, 955, 2342, 2433, 2545, 2578
PspPI GGNCC 2 cut(s) 2381, 3003
PspXI VCTCGAGB 3 cut(s) 59, 1745, 2345
PsrI GAACNNNNNNTAC 6 cut(s) 785, 817, 1016, 1048, 2424, 2456
PstI CTGCAG 1 cut(s) 681
PstNI CAGNNNCTG 1 cut(s) 2252
PsuI RGATCY 2 cut(s) 523, 2329
RruI TCGCGA 1 cut(s) 147
RseI CAYNNNNRTG 3 cut(s) 1142, 3102, 3423
SatI GCNGC 8 cut(s) 56, 89, 107, 784, 1593, 1626, 2604, 2922
Sau96I GGNCC 2 cut(s) 2381, 3003
ScaI AGTACT 1 cut(s) 989
SchI GAGTC 7 cut(s) 108, 222, 637, 1574, 1616, 2119, 3548
ScrFI CCNGG 3 cut(s) 1934, 2548, 3561
SduI GDGCHC 2 cut(s) 2548, 3333
SfaNI GCATC 9 cut(s) 665, 770, 1447, 1514, 1604, 2063, 2145, 2439, 2631
SfcI CTRYAG 4 cut(s) 183, 677, 1939, 3504
Sfr274I CTCGAG 3 cut(s) 59, 1745, 2345
SfuI TTCGAA 1 cut(s) 1235
SlaI CTCGAG 3 cut(s) 59, 1745, 2345
SmiMI CAYNNNNRTG 3 cut(s) 1142, 3102, 3423
SmlI CTYRAG 4 cut(s) 59, 1745, 2345, 2461
SmoI CTYRAG 4 cut(s) 59, 1745, 2345, 2461
SnaBI TACGTA 1 cut(s) 1922
SphI GCATGC 1 cut(s) 2205
SspI AATATT 1 cut(s) 3033
SspMI CTAG 4 cut(s) 1227, 2133, 2658, 3051
StyD4I CCNGG 3 cut(s) 1932, 2546, 3559
StyI CCWWGG 5 cut(s) 268, 461, 840, 2427, 2710
TaiI ACGT 3 cut(s) 327, 1924, 3002
TaqI TCGA 8 cut(s) 23, 29, 60, 1235, 1452, 1746, 2346, 2494
TaqII GACCGA 1 cut(s) 6
TatI WGTACW 9 cut(s) 198, 705, 881, 987, 1413, 1546, 1757, 1812, 3296
TauI GCSGC 3 cut(s) 58, 91, 109
TfiI GAWTC 8 cut(s) 13, 273, 310, 1685, 1732, 2767, 3074, 3569
TseFI GTSAC 4 cut(s) 1357, 2282, 2521, 3046
TseI GCWGC 5 cut(s) 783, 1592, 1625, 2603, 2921
Tsp45I GTSAC 4 cut(s) 1357, 2282, 2521, 3046
TspGWI ACGGA 2 cut(s) 764, 812
XapI RAATTY 9 cut(s) 167, 178, 580, 662, 1151, 1237, 1514, 1633, 1982
XceI RCATGY 1 cut(s) 2205
XhoI CTCGAG 3 cut(s) 59, 1745, 2345
XmnI GAANNNNTTC 4 cut(s) 389, 456, 2106, 2231
XspI CTAG 4 cut(s) 1227, 2133, 2658, 3051
ZrmI AGTACT 1 cut(s) 989
Zsp2I ATGCAT 1 cut(s) 2203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.