pycom05g29840

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002149
Physical Location & Seq
Forward (+)
30187137 .. 30190541
3405 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1440 bp
ATGAACCAGAAGGCTAGCGTTTCAAAAGAGCTTAATGCCAAACACAGAAAGATATTGGAGGGTCTTCTTAAACTACAAGATAACAGAGAATGCGCAGACTGCAAAACCAAAGCTCCACGATGGGCCAGCGTGAATCTTGGTATCTTCATATGCATGCAATGTTCAGGGATCCACAGGAGTCTTGGGGTACATATATCACAGGTGAGATCTGCGACTTTGGATACATGGCTCCCAGAGCAGGTTGCGTTTATCCAATCTATGGGAAACGATAAAGCAAATAGTTATTGGGAAGCCGAGTTGCCTCCTAAGTATGACAGAGTTGGAATCGAGAATTTCATTCGAGCAAAGTATGAACACAAAAAATGGGTTCCTAGGGATGGAAAAGCGAAATCACCTGCGAGGGTGAGTGAGGCAATGACTTCCGTTTATAGAGCGAGACCTGAAACTAGTACTCAACATGTCCATTTGAAAAAGATGGAACAGTTTTCTGAGGAGCAGAAAGGTGCTCGTACACACAGTACAAATAACTTGACAACGCAGCAGATCAGTCATGGTCCAAAACAACAAGAACTTGTGCAGAAACCAAAACAACAAGAACTTGATCAGAAACCTAAACCACAAGAACTTGCACAGGAACCAAAACCAGTAGTCTTGAAAGTTGAACCAGCAAGTGAGGCAGGTAGTGTTAGGCCCGTTTCGGAACCAGTTAAAGTGGATTATGCTACTCATTTGTTCAATTTGCTATCCATGGAGGAAGCTATAGAAAATGACTCGGTGATACCTGCCAATACGAATACATTGGCTGGTAACCAGGTTGAAACAAAGTTGACAGAAGAGAAAAGTAAAAGGCCAGAATCATCCGAAAGCAAGATTCAACCAAAATATGGTGGTCTCGAGGATTTATTTGTACATCCAACCCCCGACACTCAACCGTTTTCAATGAAACCTCAGAATTATGTGCAGAATGACCTTATGAACTTATTCAAGAAGTCCAGCGTGGCTTCTCCATCCTCTGTGCATCAGCAGCAACTTGCAATGACATCCCAACAACAAGCATTACCCATGGCGACTGTGGTGAAATCTAGTGGATCCCATTCCGCTCCTGCATACGTACAACCTGGCTCCCATTCTATCCAGCACTTTGCTGCCCGAAATGGGCAAAGCATTGGTCGCCAAGTTCCTGGATACCTGGTACCTGTTCATCCACAACATTATCCTCAGATTGGAAACAGCCATCATACACGGCCGGTAGGGAATACAGTTCCTTTCCAAACATCAAGCATGTACTGGACGAGGCCAGTAACTCCAATTAATGGTGTCACAAACATCGGAGCTAACGGATTTTCATCAGCGTCACCAATATCTTCTTTCCCTTCAATAAAGTCAGGAAGCGATTTTGATTTCTCATCATTAACTCAAGGATTGTTTACCAAACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap

Protein Analysis

480

Amino Acids

53.17

Weight (kDa)

9.33

Isoelectric Point (pI)

48.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 17 - 123 6.5e-41 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 403
Acc16I TGCGCA 1 cut(s) 94
Acc36I ACCTGC 4 cut(s) 229, 403, 668, 790
Acc65I GGTACC 1 cut(s) 1192
AccB1I GGYRCC 1 cut(s) 1192
AccB7I CCANNNNNTGG 3 cut(s) 259, 884, 1313
AccBSI CCGCTC 1 cut(s) 1100
AciI CCGC 1 cut(s) 1098
AclWI GGATC 4 cut(s) 163, 176, 1083, 1096
AcoI YGGCCR 1 cut(s) 1244
AcsI RAATTY 1 cut(s) 331
AfaI GTAC 8 cut(s) 189, 451, 511, 520, 909, 1113, 1194, 1286
AfiI CCNNNNNNNGG 7 cut(s) 238, 259, 377, 884, 1155, 1223, 1313
AflIII ACRYGT 1 cut(s) 457
AhlI ACTAGT 1 cut(s) 446
AjnI CCWGG 4 cut(s) 810, 1117, 1180, 1188
AluBI AGCT 4 cut(s) 31, 113, 758, 1334
AluI AGCT 4 cut(s) 31, 113, 758, 1334
Alw21I GWGCWC 1 cut(s) 508
Alw26I GTCTC 2 cut(s) 430, 896
AlwI GGATC 4 cut(s) 163, 176, 1083, 1096
Ama87I CYCGRG 1 cut(s) 893
AoxI GGCC 5 cut(s) 123, 689, 848, 1244, 1295
ApeKI GCWGC 3 cut(s) 538, 1024, 1145
ApoI RAATTY 1 cut(s) 331
ArsI GACNNNNNNTTYG 2 cut(s) 1153, 1185
AseI ATTAAT 1 cut(s) 1311
Asp700I GAANNNNTTC 1 cut(s) 980
Asp718I GGTACC 1 cut(s) 1192
AspA2I CCTAGG 1 cut(s) 371
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 3 cut(s) 123, 554, 690
AsuHPI GGTGA 6 cut(s) 214, 384, 415, 787, 1087, 1347
AsuNHI GCTAGC 1 cut(s) 14
AvaI CYCGRG 1 cut(s) 893
AvaII GGWCC 1 cut(s) 554
AvrII CCTAGG 1 cut(s) 371
BamHI GGATCC 2 cut(s) 168, 1088
BanI GGYRCC 1 cut(s) 1192
BbsI GAAGAC 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 508
BbvI GCAGC 3 cut(s) 550, 1036, 1132
BccI CCATC 5 cut(s) 114, 371, 469, 1015, 1242
BceAI ACGGC 1 cut(s) 1259
BciT130I CCWGG 4 cut(s) 812, 1119, 1182, 1190
BciVI GTATCC 2 cut(s) 214, 1178
BclI TGATCA 1 cut(s) 601
BcoDI GTCTC 2 cut(s) 430, 896
BcuI ACTAGT 1 cut(s) 446
BfaI CTAG 4 cut(s) 15, 372, 447, 1083
BfmI CTRYAG 1 cut(s) 759
BfuAI ACCTGC 4 cut(s) 229, 403, 668, 790
BfuI GTATCC 2 cut(s) 214, 1178
BglII AGATCT 1 cut(s) 206
BisI GCNGC 3 cut(s) 539, 1025, 1146
BlnI CCTAGG 1 cut(s) 371
BlsI GCNGC 3 cut(s) 540, 1026, 1147
BmcAI AGTACT 1 cut(s) 451
Bme1390I CCNGG 4 cut(s) 812, 1119, 1182, 1190
Bme18I GGWCC 1 cut(s) 554
BmeT110I CYCGRG 1 cut(s) 893
BmgT120I GGNCC 3 cut(s) 123, 554, 690
BmiI GGNNCC 8 cut(s) 170, 230, 369, 636, 702, 1090, 1123, 1194
BmrFI CCNGG 4 cut(s) 812, 1119, 1182, 1190
BmsI GCATC 1 cut(s) 1027
BmtI GCTAGC 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 1401
BsaAI YACGTR 1 cut(s) 1111
BsaBI GATNNNNATC 1 cut(s) 1345
BsaI GGTCTC 2 cut(s) 430, 896
BsaJI CCNNGG 3 cut(s) 371, 747, 1062
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bsc4I CCNNNNNNNGG 7 cut(s) 238, 259, 377, 884, 1155, 1223, 1313
Bse118I RCCGGY 1 cut(s) 1246
Bse1I ACTGG 4 cut(s) 644, 704, 1292, 1298
Bse3DI GCAATG 3 cut(s) 164, 420, 1041
Bse8I GATNNNNATC 1 cut(s) 1345
BseBI CCWGG 4 cut(s) 812, 1119, 1182, 1190
BseDI CCNNGG 3 cut(s) 371, 747, 1062
BseGI GGATG 6 cut(s) 382, 857, 910, 1007, 1040, 1201
BseJI GATNNNNATC 1 cut(s) 1345
BseLI CCNNNNNNNGG 7 cut(s) 238, 259, 377, 884, 1155, 1223, 1313
BseMI GCAATG 3 cut(s) 164, 420, 1041
BseMII CTCAG 3 cut(s) 480, 962, 1232
BseNI ACTGG 4 cut(s) 644, 704, 1292, 1298
BseRI GAGGAG 1 cut(s) 506
BseX3I CGGCCG 1 cut(s) 1244
BseXI GCAGC 3 cut(s) 550, 1036, 1132
BsgI GTGCAG 2 cut(s) 596, 980
Bsh1285I CGRYCG 1 cut(s) 1247
BshFI GGCC 5 cut(s) 125, 691, 850, 1246, 1297
BshNI GGYRCC 1 cut(s) 1192
BsiEI CGRYCG 1 cut(s) 1247
BsiHKAI GWGCWC 1 cut(s) 508
BsiHKCI CYCGRG 1 cut(s) 893
BsiSI CCGG 1 cut(s) 1247
BslI CCNNNNNNNGG 7 cut(s) 238, 259, 377, 884, 1155, 1223, 1313
BsmAI GTCTC 2 cut(s) 430, 896
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 5 cut(s) 125, 691, 850, 1246, 1297
Bso31I GGTCTC 2 cut(s) 430, 896
BsoBI CYCGRG 1 cut(s) 893
Bsp1286I GDGCHC 1 cut(s) 508
Bsp1407I TGTACA 1 cut(s) 907
Bsp143I GATC 5 cut(s) 168, 206, 543, 601, 1088
Bsp19I CCATGG 2 cut(s) 747, 1062
BspACI CCGC 1 cut(s) 1098
BspANI GGCC 5 cut(s) 125, 691, 850, 1246, 1297
BspCNI CTCAG 3 cut(s) 481, 961, 1231
BspLI GGNNCC 8 cut(s) 170, 230, 369, 636, 702, 1090, 1123, 1194
BspMI ACCTGC 4 cut(s) 229, 403, 668, 790
BspOI GCTAGC 1 cut(s) 18
BspPI GGATC 4 cut(s) 163, 176, 1083, 1096
BspT107I GGYRCC 1 cut(s) 1192
BspTNI GGTCTC 2 cut(s) 430, 896
BsrBI CCGCTC 1 cut(s) 1100
BsrDI GCAATG 3 cut(s) 164, 420, 1041
BsrFI RCCGGY 1 cut(s) 1246
BsrGI TGTACA 1 cut(s) 907
BsrI ACTGG 4 cut(s) 644, 704, 1292, 1298
BssAI RCCGGY 1 cut(s) 1246
BssECI CCNNGG 3 cut(s) 371, 747, 1062
BssMI GATC 5 cut(s) 168, 206, 543, 601, 1088
BssT1I CCWWGG 3 cut(s) 371, 747, 1062
Bst2UI CCWGG 4 cut(s) 812, 1119, 1182, 1190
Bst4CI ACNGT 6 cut(s) 483, 518, 933, 1072, 1261, 1437
Bst6I CTCTTC 1 cut(s) 828
BstAUI TGTACA 1 cut(s) 907
BstBAI YACGTR 1 cut(s) 1111
BstC8I GCNNGC 3 cut(s) 16, 127, 155
BstDEI CTNAG 4 cut(s) 306, 489, 948, 1218
BstDSI CCRYGG 2 cut(s) 747, 1062
BstEII GGTNACC 1 cut(s) 806
BstF5I GGATG 6 cut(s) 382, 857, 910, 1007, 1040, 1201
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 5 cut(s) 171, 209, 546, 604, 1091
BstMAI GTCTC 2 cut(s) 430, 896
BstMBI GATC 5 cut(s) 168, 206, 543, 601, 1088
BstMCI CGRYCG 1 cut(s) 1247
BstMWI GCNNNNNNNGC 5 cut(s) 99, 235, 674, 1024, 1170
BstNI CCWGG 4 cut(s) 812, 1119, 1182, 1190
BstNSI RCATGY 3 cut(s) 157, 461, 1285
BstPI GGTNACC 1 cut(s) 806
BstSCI CCNGG 4 cut(s) 810, 1117, 1180, 1188
BstSFI CTRYAG 1 cut(s) 759
BstSNI TACGTA 1 cut(s) 1111
BstV1I GCAGC 3 cut(s) 550, 1036, 1132
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 3 cut(s) 168, 206, 1088
BstXI CCANNNNNNTGG 1 cut(s) 1181
BstYI RGATCY 3 cut(s) 168, 206, 1088
BstZI CGGCCG 1 cut(s) 1244
BsuI GTATCC 2 cut(s) 214, 1178
BsuRI GGCC 5 cut(s) 125, 691, 850, 1246, 1297
BtgI CCRYGG 2 cut(s) 747, 1062
BtsCI GGATG 6 cut(s) 382, 857, 910, 1007, 1040, 1201
BveI ACCTGC 4 cut(s) 229, 403, 668, 790
Cac8I GCNNGC 3 cut(s) 16, 127, 155
CfoI GCGC 1 cut(s) 95
Cfr10I RCCGGY 1 cut(s) 1246
Cfr13I GGNCC 3 cut(s) 123, 554, 690
CseI GACGC 1 cut(s) 1341
CsiI ACCWGGT 2 cut(s) 810, 1188
Csp6I GTAC 8 cut(s) 188, 450, 510, 519, 908, 1112, 1193, 1285
CviAII CATG 7 cut(s) 154, 225, 458, 551, 748, 1063, 1282
CviQI GTAC 8 cut(s) 188, 450, 510, 519, 908, 1112, 1193, 1285
DdeI CTNAG 4 cut(s) 306, 489, 948, 1218
DpnI GATC 5 cut(s) 170, 208, 545, 603, 1090
DpnII GATC 5 cut(s) 168, 206, 543, 601, 1088
EaeI YGGCCR 1 cut(s) 1244
EagI CGGCCG 1 cut(s) 1244
Eam1104I CTCTTC 1 cut(s) 828
EarI CTCTTC 1 cut(s) 828
EclXI CGGCCG 1 cut(s) 1244
Eco105I TACGTA 1 cut(s) 1111
Eco130I CCWWGG 3 cut(s) 371, 747, 1062
Eco31I GGTCTC 2 cut(s) 430, 896
Eco47I GGWCC 1 cut(s) 554
Eco52I CGGCCG 1 cut(s) 1244
Eco88I CYCGRG 1 cut(s) 893
Eco91I GGTNACC 1 cut(s) 806
EcoO65I GGTNACC 1 cut(s) 806
EcoRII CCWGG 4 cut(s) 810, 1117, 1180, 1188
EcoT14I CCWWGG 3 cut(s) 371, 747, 1062
EcoT22I ATGCAT 1 cut(s) 155
ErhI CCWWGG 3 cut(s) 371, 747, 1062
FaeI CATG 7 cut(s) 157, 228, 461, 554, 751, 1066, 1285
FatI CATG 7 cut(s) 153, 224, 457, 550, 747, 1062, 1281
FauNDI CATATG 1 cut(s) 149
FbaI TGATCA 1 cut(s) 601
Fnu4HI GCNGC 3 cut(s) 539, 1025, 1146
FokI GGATG 6 cut(s) 389, 844, 897, 994, 1027, 1188
Fsp4HI GCNGC 3 cut(s) 539, 1025, 1146
FspBI CTAG 4 cut(s) 15, 372, 447, 1083
FspI TGCGCA 1 cut(s) 94
GlaI GCGC 1 cut(s) 94
GluI GCNGC 3 cut(s) 539, 1025, 1146
HaeIII GGCC 5 cut(s) 125, 691, 850, 1246, 1297
HapII CCGG 1 cut(s) 1247
HgaI GACGC 1 cut(s) 1341
HhaI GCGC 1 cut(s) 95
Hin1II CATG 7 cut(s) 157, 228, 461, 554, 751, 1066, 1285
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HincII GTYRAC 1 cut(s) 828
HindII GTYRAC 1 cut(s) 828
HinfI GANTC 6 cut(s) 133, 178, 324, 770, 854, 871
HpaII CCGG 1 cut(s) 1247
HphI GGTGA 6 cut(s) 214, 384, 415, 787, 1087, 1347
Hpy166II GTNNAC 3 cut(s) 512, 828, 1428
Hpy188I TCNGA 7 cut(s) 490, 606, 700, 862, 951, 1221, 1331
Hpy188III TCNNGA 5 cut(s) 328, 652, 893, 985, 1386
Hpy8I GTNNAC 3 cut(s) 512, 828, 1428
HpyAV CCTTC 2 cut(s) 4, 1383
HpyCH4III ACNGT 6 cut(s) 483, 518, 933, 1072, 1261, 1437
HpyCH4IV ACGT 1 cut(s) 1110
HpyCH4V TGCA 9 cut(s) 102, 153, 157, 577, 629, 961, 1018, 1034, 1106
HpyF10VI GCNNNNNNNGC 5 cut(s) 99, 235, 674, 1024, 1170
HpyF3I CTNAG 4 cut(s) 306, 489, 948, 1218
HpySE526I ACGT 1 cut(s) 1110
Hsp92II CATG 7 cut(s) 157, 228, 461, 554, 751, 1066, 1285
HspAI GCGC 1 cut(s) 93
KpnI GGTACC 1 cut(s) 1196
Ksp22I TGATCA 1 cut(s) 601
Kzo9I GATC 5 cut(s) 168, 206, 543, 601, 1088
LmnI GCTCC 6 cut(s) 118, 234, 493, 1105, 1127, 1331
Lsp1109I GCAGC 3 cut(s) 550, 1036, 1132
LweI GCATC 1 cut(s) 1027
MabI ACCWGGT 2 cut(s) 810, 1188
MaeI CTAG 4 cut(s) 15, 372, 447, 1083
MaeII ACGT 1 cut(s) 1110
MaeIII GTNAC 4 cut(s) 806, 1300, 1318, 1353
MalI GATC 5 cut(s) 170, 208, 545, 603, 1090
MbiI CCGCTC 1 cut(s) 1100
MboI GATC 5 cut(s) 168, 206, 543, 601, 1088
MboII GAAGA 4 cut(s) 56, 136, 845, 1356
MflI RGATCY 3 cut(s) 168, 206, 1088
MhlI GDGCHC 1 cut(s) 508
MluCI AATT 4 cut(s) 331, 736, 952, 1308
MlyI GAGTC 2 cut(s) 187, 764
MmeI TCCRAC 2 cut(s) 301, 938
Mph1103I ATGCAT 1 cut(s) 155
MroXI GAANNNNTTC 1 cut(s) 980
MseI TTAA 5 cut(s) 33, 69, 708, 1311, 1412
MslI CAYNNNNRTG 1 cut(s) 152
MspI CCGG 1 cut(s) 1247
MspR9I CCNGG 4 cut(s) 812, 1119, 1182, 1190
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 4 cut(s) 812, 1119, 1182, 1190
MwoI GCNNNNNNNGC 5 cut(s) 99, 235, 674, 1024, 1170
NcoI CCATGG 2 cut(s) 747, 1062
NdeI CATATG 1 cut(s) 149
NdeII GATC 5 cut(s) 168, 206, 543, 601, 1088
NheI GCTAGC 1 cut(s) 14
NlaIII CATG 7 cut(s) 157, 228, 461, 554, 751, 1066, 1285
NlaIV GGNNCC 8 cut(s) 170, 230, 369, 636, 702, 1090, 1123, 1194
NmeAIII GCCGAG 1 cut(s) 319
NmuCI GTSAC 2 cut(s) 1318, 1353
NsbI TGCGCA 1 cut(s) 94
NsiI ATGCAT 1 cut(s) 155
NspI RCATGY 3 cut(s) 157, 461, 1285
PaeI GCATGC 1 cut(s) 157
PaeR7I CTCGAG 1 cut(s) 893
PaqCI CACCTGC 1 cut(s) 403
PciI ACATGT 1 cut(s) 457
PctI GAATGC 1 cut(s) 95
PdmI GAANNNNTTC 1 cut(s) 980
PfeI GAWTC 4 cut(s) 133, 324, 854, 871
PflMI CCANNNNNTGG 3 cut(s) 259, 884, 1313
PfoI TCCNGGA 1 cut(s) 1180
PkrI GCNGC 3 cut(s) 540, 1026, 1147
PleI GAGTC 2 cut(s) 186, 764
PpsI GAGTC 2 cut(s) 186, 764
Ppu21I YACGTR 1 cut(s) 1111
PscI ACATGT 1 cut(s) 457
PshBI ATTAAT 1 cut(s) 1311
Psp6I CCWGG 4 cut(s) 810, 1117, 1180, 1188
PspEI GGTNACC 1 cut(s) 806
PspGI CCWGG 4 cut(s) 810, 1117, 1180, 1188
PspN4I GGNNCC 8 cut(s) 170, 230, 369, 636, 702, 1090, 1123, 1194
PspPI GGNCC 3 cut(s) 123, 554, 690
PsuI RGATCY 3 cut(s) 168, 206, 1088
RsaI GTAC 8 cut(s) 189, 451, 511, 520, 909, 1113, 1194, 1286
RsaNI GTAC 8 cut(s) 188, 450, 510, 519, 908, 1112, 1193, 1285
RseI CAYNNNNRTG 1 cut(s) 152
SaqAI TTAA 5 cut(s) 33, 69, 708, 1311, 1412
SatI GCNGC 3 cut(s) 539, 1025, 1146
Sau3AI GATC 5 cut(s) 168, 206, 543, 601, 1088
Sau96I GGNCC 3 cut(s) 123, 554, 690
ScaI AGTACT 1 cut(s) 451
SchI GAGTC 2 cut(s) 187, 764
ScrFI CCNGG 4 cut(s) 812, 1119, 1182, 1190
SduI GDGCHC 1 cut(s) 508
SexAI ACCWGGT 2 cut(s) 810, 1188
SfaNI GCATC 1 cut(s) 1027
SfcI CTRYAG 1 cut(s) 759
Sfr274I CTCGAG 1 cut(s) 893
SinI GGWCC 1 cut(s) 554
SlaI CTCGAG 1 cut(s) 893
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 2 cut(s) 893, 1416
SmoI CTYRAG 2 cut(s) 893, 1416
SnaBI TACGTA 1 cut(s) 1111
SpeI ACTAGT 1 cut(s) 446
SphI GCATGC 1 cut(s) 157
Sse9I AATT 4 cut(s) 331, 736, 952, 1308
SsiI CCGC 1 cut(s) 1098
SspMI CTAG 4 cut(s) 15, 372, 447, 1083
StyD4I CCNGG 4 cut(s) 810, 1117, 1180, 1188
StyI CCWWGG 3 cut(s) 371, 747, 1062
TaaI ACNGT 6 cut(s) 483, 518, 933, 1072, 1261, 1437
TaiI ACGT 1 cut(s) 1113
TaqI TCGA 3 cut(s) 327, 340, 894
TasI AATT 4 cut(s) 331, 736, 952, 1308
TatI WGTACW 4 cut(s) 449, 518, 907, 1284
TfiI GAWTC 4 cut(s) 133, 324, 854, 871
Tru1I TTAA 5 cut(s) 33, 69, 708, 1311, 1412
Tru9I TTAA 5 cut(s) 33, 69, 708, 1311, 1412
TseFI GTSAC 2 cut(s) 1318, 1353
TseI GCWGC 3 cut(s) 538, 1024, 1145
Tsp45I GTSAC 2 cut(s) 1318, 1353
TspDTI ATGAA 8 cut(s) 17, 136, 325, 366, 956, 989, 1190, 1335
TspGWI ACGGA 2 cut(s) 412, 1353
Van91I CCANNNNNTGG 3 cut(s) 259, 884, 1313
VpaK11BI GGWCC 1 cut(s) 554
VspI ATTAAT 1 cut(s) 1311
XapI RAATTY 1 cut(s) 331
XceI RCATGY 3 cut(s) 157, 461, 1285
XcmI CCANNNNNNNNNTGG 2 cut(s) 179, 1069
XhoI CTCGAG 1 cut(s) 893
XmaJI CCTAGG 1 cut(s) 371
XmnI GAANNNNTTC 1 cut(s) 980
XspI CTAG 4 cut(s) 15, 372, 447, 1083
ZrmI AGTACT 1 cut(s) 451
Zsp2I ATGCAT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.