pycom05g32470

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008028
Physical Location & Seq
Forward (+)
31880375 .. 31882085
1711 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32470.1

Sequence Viewer

Length: 1371 bp
ATGGTTTCTCTACAAGATTCCCATTCCGGCTCCAGCCACCACTTCCCGCCTCACACACGCAACTTCTACTCTCCTTCCTCCACCAAAATCCACCGTCAAACCGGCCGCTCCATGCGCACCATCCGCTCCAACATCTACCAAGACGACCACTCCCGCACCTCCTTCGCCGCCGAAAGATCGCCTTTTGTCTCCGAGAACCTGACTGATTCGGTCGTCGACATGCGCCTCGGCGAGCTCGCCCTCCGCACCCACAGCTCCGCCAGCAAGTCTGCGTCTTCCGACGAGGAGTACCTCCAGCTCTCCCAAGCCTTCAGTGATTTCTCCGCCTGCAGCAGCGACATATCGGGAGAATTGCAACGGCTCGCAAGCTTGCCGTCGCCGGAAAACGCGCCGACCTCCGAATTATCCGAGGCTGCGCCGGAACCGGAGCCATGCCAGGGGTTTTTGCAGAGGGAGAACTTCTCCACTGAGATCATCGAGAGCATTTCGCCGGAGGATCTTCAGCCGACGGTCAAGATCTGCGTCGACGGCCTCCAATCGACGTCGCTTGCCGTGAAGCAATCTGCAGCGGCCAAGCTCAGGCTTTTAGCGAAGAATCGGGCTGATAATCGCGCCTTGATCGGCGAGTCCGGCGCCGTTCCCGCTCTAATTCCTCTACTGCGGTGCAGCGATCCATGGACGCAGGAGCACGCCGTCACGGCGCTGTTAAACCTCTCGCTGCACGAATCTAACAAGGCCATAATCACCAATGCCGGAGCTATAAAGTCGTTAGTGTACGTCCTCAAGACGGGGACGGAAACCTCGAAGCAAAACGCCGCCTGTGCGCTGCTGAGCTTGGCGCTAATTGAAGAGAACAAGAGCTCAATCGGAGCCTGCGGTGCAATTCCCCCGCTGGTTTCGCTGTTAATAAGTGAATCCATGAGGGGCAAAAAGGACGCTTTGACTACTCTGTACAAGCTCTGCTCGATAAAGCCCAACAAAGAGAGGGCGGTGAGCGCGGGAGCTGTGAAGCCGCTGGTGGCACTGGTGGCGGAGCAGGGAACGGGCTTGGCGGAGAAGGCGATGGTTGTGCTGAGTAGCTTGGCGGGAGTCGAGGAGGGGAAGGAGGCTATTGTGGAGGAAGGCGGGCTGGCGGCGCTTGTGGAGGCGGTTGAAGATGGGTCGGTGAAAGGAAAGGAGTTTGCGGTGTTGACACTGTTGCAGCTGTGCAGTGAGGGTGTGAAGAACAGAGGATTGCTTGTGAGGGAAGGAGGGATTCCGCCATTGGTTGCGCTTTCGCAGACTGGGACTGTTAAAGCTAAGCACAAGGCTGAAACGCTTCTTGGGTACTTGAGAGAACCGAGACAAGAAGCGTCCTCTTCGGCTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm

Protein Analysis

457

Amino Acids

48.48

Weight (kDa)

6.0

Isoelectric Point (pI)

50.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 187 - 444 3.9e-23 Plant U-box protein ARM repeat region
Arm PF00514 202 - 239 7.8e-08 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 234 - 434 3e-16 ARMC5
Arm PF00514 284 - 322 4.2e-06 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 545
Acc16I TGCGCA 1 cut(s) 116
AccB1I GGYRCC 1 cut(s) 632
AccBSI CCGCTC 3 cut(s) 108, 126, 644
AccI GTMKAC 2 cut(s) 216, 525
AccII CGCG 3 cut(s) 389, 612, 998
AclWI GGATC 2 cut(s) 504, 665
AcoI YGGCCR 2 cut(s) 103, 570
AcuI CTGAAG 2 cut(s) 295, 485
AcyI GRCGYC 2 cut(s) 542, 633
AfaI GTAC 4 cut(s) 290, 776, 953, 1328
AfiI CCNNNNNNNGG 3 cut(s) 437, 579, 787
AgsI TTSAA 2 cut(s) 848, 1154
AjnI CCWGG 1 cut(s) 435
AjuI GAANNNNNNNTTGG 2 cut(s) 1305, 1337
Alw21I GWGCWC 3 cut(s) 237, 690, 863
Alw26I GTCTC 2 cut(s) 193, 1336
AlwI GGATC 2 cut(s) 504, 665
AoxI GGCC 4 cut(s) 103, 529, 570, 735
ApeKI GCWGC 8 cut(s) 330, 333, 413, 566, 666, 718, 826, 1201
Asp700I GAANNNNTTC 1 cut(s) 1317
AsuHPI GGTGA 3 cut(s) 736, 1003, 1177
BanI GGYRCC 1 cut(s) 632
BanII GRGCYC 2 cut(s) 237, 863
BbsI GAAGAC 1 cut(s) 267
Bbv12I GWGCWC 3 cut(s) 237, 690, 863
BbvI GCAGC 8 cut(s) 342, 345, 400, 578, 678, 705, 813, 1213
BccI CCATC 3 cut(s) 128, 1057, 1151
BceAI ACGGC 7 cut(s) 358, 374, 536, 544, 620, 677, 714
BcgI CGANNNNNNTGC 4 cut(s) 145, 179, 1051, 1085
BciT130I CCWGG 1 cut(s) 437
BcoDI GTCTC 2 cut(s) 193, 1336
BfmI CTRYAG 2 cut(s) 328, 564
BfoI RGCGCY 4 cut(s) 636, 704, 842, 1139
BglI GCCNNNNNGGC 1 cut(s) 698
BglII AGATCT 1 cut(s) 516
BlpI GCTNAGC 2 cut(s) 830, 1299
Bme1390I CCNGG 1 cut(s) 437
BmiI GGNNCC 5 cut(s) 31, 423, 429, 634, 871
BmrFI CCNGG 1 cut(s) 437
BmrI ACTGGG 1 cut(s) 1293
BmuI ACTGGG 1 cut(s) 1293
BpiI GAAGAC 1 cut(s) 267
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BpmI CTGGAG 2 cut(s) 16, 278
Bpu10I CCTNAGC 1 cut(s) 578
Bpu1102I GCTNAGC 2 cut(s) 830, 1299
BpuEI CTTGAG 2 cut(s) 767, 1351
BsaHI GRCGYC 2 cut(s) 542, 633
BsaJI CCNNGG 4 cut(s) 226, 408, 436, 674
BsaWI WCCGGW 1 cut(s) 424
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 3 cut(s) 437, 579, 787
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 2 cut(s) 1029, 1288
BseBI CCWGG 1 cut(s) 437
BseDI CCNNGG 4 cut(s) 226, 408, 436, 674
BseGI GGATG 1 cut(s) 120
BseLI CCNNNNNNNGG 3 cut(s) 437, 579, 787
BseMII CTCAG 4 cut(s) 459, 592, 821, 1064
BseNI ACTGG 2 cut(s) 1029, 1288
BseRI GAGGAG 2 cut(s) 299, 1109
BseX3I CGGCCG 1 cut(s) 103
BseXI GCAGC 8 cut(s) 342, 345, 400, 578, 678, 705, 813, 1213
BsgI GTGCAG 3 cut(s) 685, 704, 1228
Bsh1236I CGCG 3 cut(s) 389, 612, 998
Bsh1285I CGRYCG 2 cut(s) 106, 213
BshFI GGCC 4 cut(s) 105, 531, 572, 737
BshNI GGYRCC 1 cut(s) 632
BsiEI CGRYCG 2 cut(s) 106, 213
BsiHKAI GWGCWC 3 cut(s) 237, 690, 863
BsiSI CCGG 8 cut(s) 27, 102, 380, 419, 425, 491, 630, 753
BslFI GGGAC 2 cut(s) 805, 1300
BslI CCNNNNNNNGG 3 cut(s) 437, 579, 787
BsmAI GTCTC 2 cut(s) 193, 1336
BsmFI GGGAC 2 cut(s) 805, 1300
BsnI GGCC 4 cut(s) 105, 531, 572, 737
Bsp1286I GDGCHC 3 cut(s) 237, 690, 863
Bsp1407I TGTACA 1 cut(s) 951
Bsp143I GATC 6 cut(s) 176, 471, 496, 516, 618, 670
Bsp1720I GCTNAGC 2 cut(s) 830, 1299
Bsp19I CCATGG 1 cut(s) 674
BspANI GGCC 4 cut(s) 105, 531, 572, 737
BspCNI CTCAG 4 cut(s) 460, 591, 822, 1065
BspFNI CGCG 3 cut(s) 389, 612, 998
BspLI GGNNCC 5 cut(s) 31, 423, 429, 634, 871
BspMAI CTGCAG 2 cut(s) 332, 568
BspPI GGATC 2 cut(s) 504, 665
BspT107I GGYRCC 1 cut(s) 632
BsrBI CCGCTC 3 cut(s) 108, 126, 644
BsrFI RCCGGY 1 cut(s) 101
BsrGI TGTACA 1 cut(s) 951
BsrI ACTGG 2 cut(s) 1029, 1288
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 4 cut(s) 226, 408, 436, 674
BssMI GATC 6 cut(s) 176, 471, 496, 516, 618, 670
BssNI GRCGYC 2 cut(s) 542, 633
BssT1I CCWWGG 1 cut(s) 674
Bst2UI CCWGG 1 cut(s) 437
Bst4CI ACNGT 4 cut(s) 95, 511, 1197, 1291
Bst6I CTCTTC 2 cut(s) 843, 1363
BstACI GRCGYC 2 cut(s) 542, 633
BstAUI TGTACA 1 cut(s) 951
BstDEI CTNAG 5 cut(s) 468, 578, 830, 1073, 1299
BstDSI CCRYGG 1 cut(s) 674
BstF5I GGATG 1 cut(s) 120
BstFNI CGCG 3 cut(s) 389, 612, 998
BstH2I RGCGCY 4 cut(s) 636, 704, 842, 1139
BstKTI GATC 6 cut(s) 179, 474, 499, 519, 621, 673
BstMAI GTCTC 2 cut(s) 193, 1336
BstMBI GATC 6 cut(s) 176, 471, 496, 516, 618, 670
BstMCI CGRYCG 2 cut(s) 106, 213
BstNI CCWGG 1 cut(s) 437
BstNSI RCATGY 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 435
BstSFI CTRYAG 2 cut(s) 328, 564
BstUI CGCG 3 cut(s) 389, 612, 998
BstV1I GCAGC 8 cut(s) 342, 345, 400, 578, 678, 705, 813, 1213
BstV2I GAAGAC 1 cut(s) 267
BstX2I RGATCY 2 cut(s) 496, 516
BstYI RGATCY 2 cut(s) 496, 516
BstZI CGGCCG 1 cut(s) 103
BsuRI GGCC 4 cut(s) 105, 531, 572, 737
BtgI CCRYGG 1 cut(s) 674
BtgZI GCGATG 1 cut(s) 1076
BtsCI GGATG 1 cut(s) 120
BtsI GCAGTG 1 cut(s) 1216
BtsIMutI CAGTG 5 cut(s) 319, 465, 1022, 1193, 1216
Cfr10I RCCGGY 1 cut(s) 101
CseI GACGC 5 cut(s) 261, 511, 688, 944, 1341
Csp6I GTAC 4 cut(s) 289, 775, 952, 1327
CviAII CATG 5 cut(s) 112, 220, 432, 675, 919
CviQI GTAC 4 cut(s) 289, 775, 952, 1327
DdeI CTNAG 5 cut(s) 468, 578, 830, 1073, 1299
DinI GGCGCC 1 cut(s) 634
DpnI GATC 6 cut(s) 178, 473, 498, 518, 620, 672
DpnII GATC 6 cut(s) 176, 471, 496, 516, 618, 670
EaeI YGGCCR 2 cut(s) 103, 570
EagI CGGCCG 1 cut(s) 103
Eam1104I CTCTTC 2 cut(s) 843, 1363
EarI CTCTTC 2 cut(s) 843, 1363
EciI GGCGGA 5 cut(s) 247, 313, 1046, 1067, 1248
Ecl136II GAGCTC 2 cut(s) 235, 861
EclXI CGGCCG 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 674
Eco24I GRGCYC 2 cut(s) 237, 863
Eco52I CGGCCG 1 cut(s) 103
Eco53kI GAGCTC 2 cut(s) 235, 861
Eco57I CTGAAG 2 cut(s) 295, 485
EcoICRI GAGCTC 2 cut(s) 235, 861
EcoRII CCWGG 1 cut(s) 435
EcoT14I CCWWGG 1 cut(s) 674
EcoT38I GRGCYC 2 cut(s) 237, 863
EgeI GGCGCC 1 cut(s) 634
EheI GGCGCC 1 cut(s) 634
ErhI CCWWGG 1 cut(s) 674
FaeI CATG 5 cut(s) 115, 223, 435, 678, 922
FaiI YATR 8 cut(s) 113, 221, 341, 433, 676, 740, 761, 920
FalI AAGNNNNNCTT 2 cut(s) 166, 198
FaqI GGGAC 2 cut(s) 805, 1300
FatI CATG 5 cut(s) 111, 219, 431, 674, 918
FauI CCCGC 7 cut(s) 54, 161, 649, 897, 991, 1078, 1118
FblI GTMKAC 2 cut(s) 216, 525
FokI GGATG 1 cut(s) 107
FriOI GRGCYC 2 cut(s) 237, 863
FspAI RTGCGCAY 1 cut(s) 116
FspI TGCGCA 1 cut(s) 116
GsuI CTGGAG 2 cut(s) 16, 278
HaeII RGCGCY 4 cut(s) 636, 704, 842, 1139
HaeIII GGCC 4 cut(s) 105, 531, 572, 737
HapII CCGG 8 cut(s) 27, 102, 380, 419, 425, 491, 630, 753
HgaI GACGC 5 cut(s) 261, 511, 688, 944, 1341
Hin1I GRCGYC 2 cut(s) 542, 633
Hin1II CATG 5 cut(s) 115, 223, 435, 678, 922
HincII GTYRAC 3 cut(s) 217, 526, 1191
HindII GTYRAC 3 cut(s) 217, 526, 1191
HindIII AAGCTT 1 cut(s) 367
HinfI GANTC 8 cut(s) 17, 206, 595, 626, 725, 914, 1089, 1255
HpaII CCGG 8 cut(s) 27, 102, 380, 419, 425, 491, 630, 753
HphI GGTGA 3 cut(s) 736, 1003, 1177
Hpy166II GTNNAC 4 cut(s) 217, 526, 775, 1191
Hpy188I TCNGA 5 cut(s) 193, 280, 400, 409, 869
Hpy188III TCNNGA 4 cut(s) 345, 478, 514, 784
Hpy8I GTNNAC 4 cut(s) 217, 526, 775, 1191
Hpy99I CGWCG 8 cut(s) 218, 284, 379, 511, 527, 530, 544, 547
HpyAV CCTTC 7 cut(s) 84, 172, 319, 1051, 1096, 1115, 1241
HpyCH4III ACNGT 4 cut(s) 95, 511, 1197, 1291
HpyCH4IV ACGT 2 cut(s) 542, 777
HpyCH4V TGCA 9 cut(s) 330, 355, 448, 566, 666, 721, 881, 1201, 1209
HpyF3I CTNAG 5 cut(s) 468, 578, 830, 1073, 1299
HpySE526I ACGT 2 cut(s) 542, 777
Hsp92I GRCGYC 2 cut(s) 542, 633
Hsp92II CATG 5 cut(s) 115, 223, 435, 678, 922
KasI GGCGCC 1 cut(s) 632
Kzo9I GATC 6 cut(s) 176, 471, 496, 516, 618, 670
Lsp1109I GCAGC 8 cut(s) 342, 345, 400, 578, 678, 705, 813, 1213
MaeII ACGT 2 cut(s) 542, 777
MaeIII GTNAC 1 cut(s) 694
MalI GATC 6 cut(s) 178, 473, 498, 518, 620, 672
MbiI CCGCTC 3 cut(s) 108, 126, 644
MboI GATC 6 cut(s) 176, 471, 496, 516, 618, 670
MboII GAAGA 7 cut(s) 267, 491, 604, 860, 1166, 1234, 1350
MflI RGATCY 2 cut(s) 496, 516
MhlI GDGCHC 3 cut(s) 237, 690, 863
MluCI AATT 5 cut(s) 350, 401, 648, 843, 882
Mly113I GGCGCC 1 cut(s) 633
MlyI GAGTC 2 cut(s) 635, 1098
MmeI TCCRAC 2 cut(s) 153, 303
MroXI GAANNNNTTC 1 cut(s) 1317
MseI TTAA 3 cut(s) 707, 905, 1293
MspA1I CMGCKG 4 cut(s) 569, 892, 1015, 1204
MspI CCGG 8 cut(s) 27, 102, 380, 419, 425, 491, 630, 753
MspR9I CCNGG 1 cut(s) 437
MvaI CCWGG 1 cut(s) 437
MvnI CGCG 3 cut(s) 389, 612, 998
NarI GGCGCC 1 cut(s) 633
NcoI CCATGG 1 cut(s) 674
NdeII GATC 6 cut(s) 176, 471, 496, 516, 618, 670
NlaIII CATG 5 cut(s) 115, 223, 435, 678, 922
NlaIV GGNNCC 5 cut(s) 31, 423, 429, 634, 871
NmeAIII GCCGAG 1 cut(s) 207
NmuCI GTSAC 1 cut(s) 694
NsbI TGCGCA 1 cut(s) 116
NspI RCATGY 1 cut(s) 223
PcsI WCGNNNNNNNCGW 1 cut(s) 800
PdmI GAANNNNTTC 1 cut(s) 1317
PfeI GAWTC 6 cut(s) 17, 206, 595, 725, 914, 1255
PleI GAGTC 2 cut(s) 634, 1097
PluTI GGCGCC 1 cut(s) 636
PpsI GAGTC 2 cut(s) 634, 1097
Psp124BI GAGCTC 2 cut(s) 237, 863
Psp6I CCWGG 1 cut(s) 435
PspGI CCWGG 1 cut(s) 435
PspN4I GGNNCC 5 cut(s) 31, 423, 429, 634, 871
PstI CTGCAG 2 cut(s) 332, 568
PsuI RGATCY 2 cut(s) 496, 516
PvuII CAGCTG 1 cut(s) 1204
RsaI GTAC 4 cut(s) 290, 776, 953, 1328
RsaNI GTAC 4 cut(s) 289, 775, 952, 1327
SacI GAGCTC 2 cut(s) 237, 863
SalI GTCGAC 2 cut(s) 215, 524
SaqAI TTAA 3 cut(s) 707, 905, 1293
Sau3AI GATC 6 cut(s) 176, 471, 496, 516, 618, 670
SchI GAGTC 2 cut(s) 635, 1098
ScrFI CCNGG 1 cut(s) 437
SduI GDGCHC 3 cut(s) 237, 690, 863
SfcI CTRYAG 2 cut(s) 328, 564
SfoI GGCGCC 1 cut(s) 634
SgrDI CGTCGACG 1 cut(s) 524
SmlI CTYRAG 2 cut(s) 782, 1330
SmoI CTYRAG 2 cut(s) 782, 1330
Sse9I AATT 5 cut(s) 350, 401, 648, 843, 882
SspDI GGCGCC 1 cut(s) 632
SstI GAGCTC 2 cut(s) 237, 863
StyD4I CCNGG 1 cut(s) 435
StyI CCWWGG 1 cut(s) 674
TaaI ACNGT 4 cut(s) 95, 511, 1197, 1291
TaiI ACGT 2 cut(s) 545, 780
TaqI TCGA 7 cut(s) 216, 477, 525, 539, 803, 965, 1092
TaqII GACCGA 1 cut(s) 199
TasI AATT 5 cut(s) 350, 401, 648, 843, 882
TatI WGTACW 1 cut(s) 951
TauI GCSGC 6 cut(s) 108, 170, 572, 818, 1015, 1136
TfiI GAWTC 6 cut(s) 17, 206, 595, 725, 914, 1255
Tru1I TTAA 3 cut(s) 707, 905, 1293
Tru9I TTAA 3 cut(s) 707, 905, 1293
TscAI CASTG 5 cut(s) 319, 472, 1029, 1200, 1216
TseFI GTSAC 1 cut(s) 694
TseI GCWGC 8 cut(s) 330, 333, 413, 566, 666, 718, 826, 1201
Tsp45I GTSAC 1 cut(s) 694
TspGWI ACGGA 1 cut(s) 809
TspRI CASTG 5 cut(s) 319, 472, 1029, 1200, 1216
XceI RCATGY 1 cut(s) 223
XmiI GTMKAC 2 cut(s) 216, 525
XmnI GAANNNNTTC 1 cut(s) 1317
ZraI GACGTC 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.