pycom06g02980

GWT1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005057
Physical Location & Seq
Reverse (-)
3780535 .. 3781680
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 309 bp
ATGGATTCTCTTCCCAACACCTTCAATCCAAACAAACGTCTGAAAGAAGAGTTTGTGAGCAATTTGAGTGGATCGTCGATGATGGAGATTGCCGCGCTTACCACGATAATACCTATTCTGATACTTCTCCGGCACTCAGTTGGCTCTCATCGTGCCGTCGATGTCGTATCGAAGAAAAATGATGATGCAGTAGTTGGTTCTAAGGGATGGGCAGCTTACATGGCTTCAATGTCTCTTGATTTTCTTCTCATTATAGTTCCAATCGTCTTAGTTTACACTAGTGGATGTATATCTGGGCAACTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
GWT1

Protein Analysis

103

Amino Acids

11.01

Weight (kDa)

6.7

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWT1 PF06423 12 - 93 9.1e-10 GWT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 95
AciI CCGC 1 cut(s) 93
AclWI GGATC 1 cut(s) 79
AgsI TTSAA 2 cut(s) 25, 228
AhlI ACTAGT 1 cut(s) 278
AluBI AGCT 1 cut(s) 215
AluI AGCT 1 cut(s) 215
Alw26I GTCTC 1 cut(s) 237
AlwI GGATC 1 cut(s) 79
ApeKI GCWGC 1 cut(s) 212
AspLEI GCGC 1 cut(s) 97
BbvI GCAGC 1 cut(s) 224
BccI CCATC 2 cut(s) 76, 201
BceAI ACGGC 1 cut(s) 140
BcoDI GTCTC 1 cut(s) 237
BcuI ACTAGT 1 cut(s) 278
BfaI CTAG 1 cut(s) 279
BisI GCNGC 2 cut(s) 93, 213
BlsI GCNGC 2 cut(s) 94, 214
BmsI GCATC 1 cut(s) 175
BsaBI GATNNNNATC 1 cut(s) 289
Bse8I GATNNNNATC 1 cut(s) 289
BseGI GGATG 2 cut(s) 212, 290
BseJI GATNNNNATC 1 cut(s) 289
BseMII CTCAG 1 cut(s) 150
BseXI GCAGC 1 cut(s) 224
Bsh1236I CGCG 1 cut(s) 95
BsiSI CCGG 1 cut(s) 130
BsmAI GTCTC 1 cut(s) 237
Bsp143I GATC 1 cut(s) 71
BspACI CCGC 1 cut(s) 93
BspCNI CTCAG 1 cut(s) 149
BspFNI CGCG 1 cut(s) 95
BspPI GGATC 1 cut(s) 79
BssMI GATC 1 cut(s) 71
Bst6I CTCTTC 2 cut(s) 15, 42
BstDEI CTNAG 3 cut(s) 136, 201, 268
BstF5I GGATG 2 cut(s) 212, 290
BstFNI CGCG 1 cut(s) 95
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 1 cut(s) 74
BstMAI GTCTC 1 cut(s) 237
BstMBI GATC 1 cut(s) 71
BstMWI GCNNNNNNNGC 1 cut(s) 221
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 1 cut(s) 224
BtsCI GGATG 2 cut(s) 212, 290
CfoI GCGC 1 cut(s) 97
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 1 cut(s) 220
CviJI RGCY 3 cut(s) 144, 215, 224
CviKI_1 RGCY 3 cut(s) 144, 215, 224
DdeI CTNAG 3 cut(s) 136, 201, 268
DpnI GATC 1 cut(s) 73
DpnII GATC 1 cut(s) 71
Eam1104I CTCTTC 2 cut(s) 15, 42
EarI CTCTTC 2 cut(s) 15, 42
FaeI CATG 1 cut(s) 223
FaiI YATR 3 cut(s) 221, 254, 290
FatI CATG 1 cut(s) 219
Fnu4HI GCNGC 2 cut(s) 93, 213
FokI GGATG 2 cut(s) 219, 297
Fsp4HI GCNGC 2 cut(s) 93, 213
FspBI CTAG 1 cut(s) 279
GlaI GCGC 1 cut(s) 96
GluI GCNGC 2 cut(s) 93, 213
HapII CCGG 1 cut(s) 130
HhaI GCGC 1 cut(s) 97
Hin1II CATG 1 cut(s) 223
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HinfI GANTC 1 cut(s) 5
HpaII CCGG 1 cut(s) 130
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 2 cut(s) 42, 120
Hpy188III TCNNGA 1 cut(s) 236
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 2 cut(s) 79, 161
HpyAV CCTTC 1 cut(s) 31
HpyCH4IV ACGT 1 cut(s) 37
HpyCH4V TGCA 1 cut(s) 188
HpyF10VI GCNNNNNNNGC 1 cut(s) 221
HpyF3I CTNAG 3 cut(s) 136, 201, 268
HpySE526I ACGT 1 cut(s) 37
Hsp92II CATG 1 cut(s) 223
HspAI GCGC 1 cut(s) 95
Kzo9I GATC 1 cut(s) 71
LpnPI CCDG 2 cut(s) 143, 279
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 1 cut(s) 175
MaeI CTAG 1 cut(s) 279
MaeII ACGT 1 cut(s) 37
MalI GATC 1 cut(s) 73
MboI GATC 1 cut(s) 71
MboII GAAGA 3 cut(s) 59, 184, 236
MluCI AATT 1 cut(s) 61
MspI CCGG 1 cut(s) 130
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 1 cut(s) 221
NdeII GATC 1 cut(s) 71
NlaIII CATG 1 cut(s) 223
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 2 cut(s) 94, 214
SatI GCNGC 2 cut(s) 93, 213
Sau3AI GATC 1 cut(s) 71
SetI ASST 4 cut(s) 23, 40, 115, 217
SfaNI GCATC 1 cut(s) 175
SgeI CNNG 7 cut(s) 106, 115, 142, 164, 232, 248, 291
SpeI ACTAGT 1 cut(s) 278
Sse9I AATT 1 cut(s) 61
SsiI CCGC 1 cut(s) 93
SspMI CTAG 1 cut(s) 279
TaiI ACGT 1 cut(s) 40
TaqI TCGA 3 cut(s) 77, 159, 170
TasI AATT 1 cut(s) 61
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 1 cut(s) 5
TseI GCWGC 1 cut(s) 212
XspI CTAG 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.