pycom06g19010

Belongs to the PTH family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0007236
Physical Location & Seq
Reverse (-)
22553080 .. 22554961
1882 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
ATGAACGTTGCTGCTTCCCTATCATCCATCGCCACCGTCCGCTTCCCCAATTGCCACCGCTGCTTCGGCTTCTCAAAACCCCTAATTCAATCTCCGTCGCTTTGGATAATGGCCCAATTCTCGTCATCGCAATCTCCCTCTGCCGCGCCGGAAACGGCGGTTGTGGAAGCCAAGACGCCCCCCAAGCAGCCGTGGCTCATCGTCGGCCTCGGAAACCCCGGGAAGAAGTACAACAGCACCCGCCACAATGTGGGTTTTGAGATGGTGGATGCCATTGCTGAAGCTGAAGGGATACCTATGAGCAGTGTTTCTTTCAAAGCCCTTGTTGGAAAAGGTCTTATTGGGGATGTTCCGGTTATATTTGCCAAACCACAAACTTACATGAATAAAAGCGGTGAGTCTGTTGGATCCATTGTGTCGTATTACAAGATTCCATTGAAGCAAGTACTTGTGATATTTGATGATCTAGATCTTCCCTTTGGAAAACTGCGGATGTTGCCGAAAGGTGGACATGGAGGACACAATGGGATGAGGAGTGTCATTGATCGCTTTAAGGGGAGTCGAGATTTTCCTCGTTTAAGAATCGGCATTGGACGCCCTCCTGGGAAAATGGATCCTGTCAACTTTGTTCTTCGTCCATTTACTAAACAAGAACAACAGGAGCTGAATTTTACATTTCAAGAAGGTGTAGAAGCGGTGCGTATTCTTTTGCTTGAGGGGTTCAACAAAAGCGCAACTTTTGTTAATAGTGCCAAACCCTTGGAACAATGCGGTTAA

Protein Analysis

259

Amino Acids

28.17

Weight (kDa)

9.63

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 66 - 246 1.1e-65 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 250
AccII CGCG 1 cut(s) 146
AciI CCGC 9 cut(s) 40, 58, 144, 158, 241, 393, 490, 695, 771
AclI AACGTT 1 cut(s) 6
AclWI GGATC 4 cut(s) 402, 415, 608, 621
AcsI RAATTY 1 cut(s) 667
AcuI CTGAAG 2 cut(s) 300, 306
AcyI GRCGYC 2 cut(s) 176, 595
AdeI CACNNNGTG 1 cut(s) 250
AfaI GTAC 2 cut(s) 230, 447
AfiI CCNNNNNNNGG 2 cut(s) 250, 506
AgsI TTSAA 5 cut(s) 89, 316, 439, 680, 724
AjnI CCWGG 1 cut(s) 601
AluBI AGCT 2 cut(s) 284, 664
AluI AGCT 2 cut(s) 284, 664
AlwI GGATC 4 cut(s) 402, 415, 608, 621
AlwNI CAGNNNCTG 1 cut(s) 664
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 2 cut(s) 111, 205
ApeKI GCWGC 3 cut(s) 11, 60, 187
ApoI RAATTY 1 cut(s) 667
AspLEI GCGC 2 cut(s) 148, 734
AspS9I GGNCC 1 cut(s) 112
AsuC2I CCSGG 2 cut(s) 219, 220
AsuHPI GGTGA 1 cut(s) 407
AvaI CYCGRG 1 cut(s) 218
BamHI GGATCC 2 cut(s) 407, 613
BbvI GCAGC 2 cut(s) 47, 199
BccI CCATC 2 cut(s) 35, 256
BceAI ACGGC 2 cut(s) 171, 175
BciT130I CCWGG 1 cut(s) 603
BciVI GTATCC 1 cut(s) 285
BcnI CCSGG 2 cut(s) 219, 220
BfaI CTAG 1 cut(s) 467
BfuI GTATCC 1 cut(s) 285
BglII AGATCT 1 cut(s) 469
BisI GCNGC 4 cut(s) 12, 61, 144, 188
BlsI GCNGC 4 cut(s) 13, 62, 145, 189
BmcAI AGTACT 1 cut(s) 447
Bme1390I CCNGG 3 cut(s) 219, 220, 603
BmeT110I CYCGRG 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 2 cut(s) 409, 615
BmrFI CCNGG 3 cut(s) 219, 220, 603
BmsI GCATC 1 cut(s) 259
BpuEI CTTGAG 1 cut(s) 734
BpuMI CCSGG 2 cut(s) 219, 220
BsaBI GATNNNNATC 1 cut(s) 468
BsaHI GRCGYC 2 cut(s) 176, 595
BsaJI CCNNGG 6 cut(s) 191, 208, 217, 218, 602, 759
BsaWI WCCGGW 1 cut(s) 352
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 506
Bse3DI GCAATG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 468
BseBI CCWGG 1 cut(s) 603
BseDI CCNNGG 6 cut(s) 191, 208, 217, 218, 602, 759
BseGI GGATG 5 cut(s) 23, 274, 352, 498, 534
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 2 cut(s) 250, 506
BseMI GCAATG 1 cut(s) 273
BseRI GAGGAG 1 cut(s) 547
BseXI GCAGC 2 cut(s) 47, 199
Bsh1236I CGCG 1 cut(s) 146
BshFI GGCC 2 cut(s) 113, 207
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 3 cut(s) 149, 219, 353
BslI CCNNNNNNNGG 2 cut(s) 250, 506
BsnI GGCC 2 cut(s) 113, 207
BsoBI CYCGRG 1 cut(s) 218
Bsp143I GATC 5 cut(s) 407, 463, 469, 544, 613
BspACI CCGC 9 cut(s) 40, 58, 144, 158, 241, 393, 490, 695, 771
BspANI GGCC 2 cut(s) 113, 207
BspFNI CGCG 1 cut(s) 146
BspLI GGNNCC 2 cut(s) 409, 615
BspPI GGATC 4 cut(s) 402, 415, 608, 621
BsrDI GCAATG 1 cut(s) 273
BssECI CCNNGG 6 cut(s) 191, 208, 217, 218, 602, 759
BssMI GATC 5 cut(s) 407, 463, 469, 544, 613
BssNI GRCGYC 2 cut(s) 176, 595
BssT1I CCWWGG 1 cut(s) 759
Bst2UI CCWGG 1 cut(s) 603
Bst4CI ACNGT 1 cut(s) 37
BstACI GRCGYC 2 cut(s) 176, 595
BstDSI CCRYGG 1 cut(s) 191
BstF5I GGATG 5 cut(s) 23, 274, 352, 498, 534
BstFNI CGCG 1 cut(s) 146
BstHHI GCGC 2 cut(s) 148, 734
BstKTI GATC 5 cut(s) 410, 466, 472, 547, 616
BstMBI GATC 5 cut(s) 407, 463, 469, 544, 613
BstMWI GCNNNNNNNGC 6 cut(s) 60, 66, 184, 193, 496, 594
BstNI CCWGG 1 cut(s) 603
BstSCI CCNGG 3 cut(s) 217, 218, 601
BstUI CGCG 1 cut(s) 146
BstV1I GCAGC 2 cut(s) 47, 199
BstX2I RGATCY 3 cut(s) 407, 469, 613
BstXI CCANNNNNNTGG 1 cut(s) 760
BstYI RGATCY 3 cut(s) 407, 469, 613
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 2 cut(s) 113, 207
BtgI CCRYGG 1 cut(s) 191
BtgZI GCGATG 2 cut(s) 13, 111
BtsCI GGATG 5 cut(s) 23, 274, 352, 498, 534
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 310
CaiI CAGNNNCTG 1 cut(s) 664
CfoI GCGC 2 cut(s) 148, 734
Cfr13I GGNCC 1 cut(s) 112
Cfr9I CCCGGG 1 cut(s) 218
CseI GACGC 2 cut(s) 184, 603
Csp6I GTAC 2 cut(s) 229, 446
CviAII CATG 2 cut(s) 382, 512
CviJI RGCY 9 cut(s) 69, 113, 170, 190, 196, 207, 284, 320, 664
CviKI_1 RGCY 9 cut(s) 69, 113, 170, 190, 196, 207, 284, 320, 664
CviQI GTAC 2 cut(s) 229, 446
DpnI GATC 5 cut(s) 409, 465, 471, 546, 615
DpnII GATC 5 cut(s) 407, 463, 469, 544, 613
DraIII CACNNNGTG 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 759
Eco57I CTGAAG 2 cut(s) 300, 306
Eco88I CYCGRG 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 601
EcoT14I CCWWGG 1 cut(s) 759
ErhI CCWWGG 1 cut(s) 759
FaeI CATG 2 cut(s) 385, 515
FaiI YATR 4 cut(s) 299, 359, 383, 513
FalI AAGNNNNNCTT 2 cut(s) 721, 753
FatI CATG 2 cut(s) 381, 511
FauI CCCGC 1 cut(s) 248
Fnu4HI GCNGC 4 cut(s) 12, 61, 144, 188
FokI GGATG 5 cut(s) 10, 281, 359, 505, 541
Fsp4HI GCNGC 4 cut(s) 12, 61, 144, 188
FspBI CTAG 1 cut(s) 467
GlaI GCGC 2 cut(s) 147, 733
GluI GCNGC 4 cut(s) 12, 61, 144, 188
HaeIII GGCC 2 cut(s) 113, 207
HapII CCGG 3 cut(s) 149, 219, 353
HgaI GACGC 2 cut(s) 184, 603
HhaI GCGC 2 cut(s) 148, 734
Hin1I GRCGYC 2 cut(s) 176, 595
Hin1II CATG 2 cut(s) 385, 515
Hin6I GCGC 2 cut(s) 146, 732
HinP1I GCGC 2 cut(s) 146, 732
HincII GTYRAC 1 cut(s) 622
HindII GTYRAC 1 cut(s) 622
HinfI GANTC 4 cut(s) 398, 430, 559, 582
HpaII CCGG 3 cut(s) 149, 219, 353
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 2 cut(s) 509, 622
Hpy188I TCNGA 1 cut(s) 212
Hpy188III TCNNGA 3 cut(s) 467, 563, 680
Hpy8I GTNNAC 2 cut(s) 509, 622
Hpy99I CGWCG 2 cut(s) 100, 206
HpyAV CCTTC 2 cut(s) 281, 677
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 1 cut(s) 6
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 66, 184, 193, 496, 594
HpySE526I ACGT 1 cut(s) 6
Hsp92I GRCGYC 2 cut(s) 176, 595
Hsp92II CATG 2 cut(s) 385, 515
HspAI GCGC 2 cut(s) 146, 732
Kzo9I GATC 5 cut(s) 407, 463, 469, 544, 613
LmnI GCTCC 1 cut(s) 661
LpnPI CCDG 7 cut(s) 162, 232, 366, 588, 615, 630, 644
Lsp1109I GCAGC 2 cut(s) 47, 199
LweI GCATC 1 cut(s) 259
MaeI CTAG 1 cut(s) 467
MaeII ACGT 1 cut(s) 6
MalI GATC 5 cut(s) 409, 465, 471, 546, 615
MboI GATC 5 cut(s) 407, 463, 469, 544, 613
MboII GAAGA 3 cut(s) 235, 464, 623
MfeI CAATTG 1 cut(s) 49
MflI RGATCY 3 cut(s) 407, 469, 613
MluCI AATT 4 cut(s) 49, 84, 116, 667
MlyI GAGTC 2 cut(s) 407, 568
MmeI TCCRAC 2 cut(s) 307, 385
MnlI CCTC 7 cut(s) 148, 218, 509, 525, 582, 609, 709
MseI TTAA 4 cut(s) 552, 578, 744, 775
MspA1I CMGCKG 1 cut(s) 60
MspI CCGG 3 cut(s) 149, 219, 353
MspR9I CCNGG 3 cut(s) 219, 220, 603
MunI CAATTG 1 cut(s) 49
MvaI CCWGG 1 cut(s) 603
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 6 cut(s) 60, 66, 184, 193, 496, 594
NciI CCSGG 2 cut(s) 219, 220
NdeII GATC 5 cut(s) 407, 463, 469, 544, 613
NlaIII CATG 2 cut(s) 385, 515
NlaIV GGNNCC 2 cut(s) 409, 615
PfeI GAWTC 2 cut(s) 430, 582
PflMI CCANNNNNTGG 1 cut(s) 250
PkrI GCNGC 4 cut(s) 13, 62, 145, 189
PleI GAGTC 2 cut(s) 406, 567
PpsI GAGTC 2 cut(s) 406, 567
Psp1406I AACGTT 1 cut(s) 6
Psp6I CCWGG 1 cut(s) 601
PspGI CCWGG 1 cut(s) 601
PspN4I GGNNCC 2 cut(s) 409, 615
PspPI GGNCC 1 cut(s) 112
PstNI CAGNNNCTG 1 cut(s) 664
PsuI RGATCY 3 cut(s) 407, 469, 613
RsaI GTAC 2 cut(s) 230, 447
RsaNI GTAC 2 cut(s) 229, 446
SaqAI TTAA 4 cut(s) 552, 578, 744, 775
SatI GCNGC 4 cut(s) 12, 61, 144, 188
Sau3AI GATC 5 cut(s) 407, 463, 469, 544, 613
Sau96I GGNCC 1 cut(s) 112
ScaI AGTACT 1 cut(s) 447
SchI GAGTC 2 cut(s) 407, 568
ScrFI CCNGG 3 cut(s) 219, 220, 603
SetI ASST 7 cut(s) 9, 286, 298, 337, 508, 666, 688
SfaNI GCATC 1 cut(s) 259
SmaI CCCGGG 1 cut(s) 220
SmlI CTYRAG 1 cut(s) 713
SmoI CTYRAG 1 cut(s) 713
Sse9I AATT 4 cut(s) 49, 84, 116, 667
SsiI CCGC 9 cut(s) 40, 58, 144, 158, 241, 393, 490, 695, 771
SspMI CTAG 1 cut(s) 467
StyD4I CCNGG 3 cut(s) 217, 218, 601
StyI CCWWGG 1 cut(s) 759
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 1 cut(s) 9
TaqI TCGA 1 cut(s) 562
TasI AATT 4 cut(s) 49, 84, 116, 667
TatI WGTACW 2 cut(s) 228, 445
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 2 cut(s) 430, 582
Tru1I TTAA 4 cut(s) 552, 578, 744, 775
Tru9I TTAA 4 cut(s) 552, 578, 744, 775
TscAI CASTG 1 cut(s) 310
TseI GCWGC 3 cut(s) 11, 60, 187
TspDTI ATGAA 2 cut(s) 17, 398
TspGWI ACGGA 1 cut(s) 84
TspMI CCCGGG 1 cut(s) 218
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 1 cut(s) 250
XapI RAATTY 1 cut(s) 667
XbaI TCTAGA 1 cut(s) 466
XmaI CCCGGG 1 cut(s) 218
XspI CTAG 1 cut(s) 467
ZrmI AGTACT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.