pycom07g00260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007084
Physical Location & Seq
Reverse (-)
272660 .. 278120
5461 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 945 bp
ATGGCCATAGATCCCAGCCACACCACCGCTTCAGCCGCCGCCGTCACGGCCACGGCCGGAGCTGCCGCTCCCAACACACCCACGACGAGGCCCGTAAGACCCGCCGTATCATCTCCTCTTCCTCCTAATCTTTACGCAGCCCAATCCCTCCAAATTCGGACCCAATCGCCATCATCTTCACCATTAGCATCAGCAGCTGCTAATCATCAGGGGGTTCTGTACCCACTGGCTTCCTCAGGCCGAGGCTTCATTCCCCGGCCCATTCGCCCCGCCGCCGCTGCCGGAGGTGAACCCCCAGTCACTGTGGCCAACGCAGGAGCTTCGTACCCATCTCGGCCCCTTCTGAATTTCCCTTCGCAGCAGCCTATTTCTGTGCATTTGATGAGATCCAACTACAGCTTAGCACCTTCTCCTCTTCCTCCTCCTATTAGGGGCCTTCCCCTTTCTTCTGCTGCTCCTGAGGTTGCTCCATCTTCAGTTCCTGACAGCAATGGTTTTAAAGACATGAGGGATAAAAACAAAGATGATAACTTAGCTGTCATTAGAGGTCGAAAAGTCAGGGTGACAGATGGAGCTTCTCTATACGTGCACTGTCGGTCATGGTTGAGGAACGGTTTTCCTGAAGAAATTCAGCCACAATATGGTGATGCTGTAAGGTCTCTTCCTAAACCTTCACCAATACCTATGGCAAGTGCTACTCTTCCAAAGAAGGAGGGGGGTGAAGCAGAAGAAAAAGGAGATGATAACAAGGACGAGGCTGAGGAACACATTGAGCGTTTATCAACACATGATCTGTTAAAAAGACATGTCAAACGCGCCAGAAAGGTTCGAGCACGATTAAGAGAAGAACGCTTACAGCGAATTGCAAGGTACAAGTCTAGGCTTGCGCTTCTCCTTCCTCCCTTGGTAGAACAGTTCAGAAATGATCTAGCTTCAGGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIN37

Protein Analysis

315

Amino Acids

33.69

Weight (kDa)

10.07

Isoelectric Point (pI)

64.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LIN37 PF15306 190 - 298 2.1e-06 LIN37
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 641
AccBSI CCGCTC 1 cut(s) 68
AccII CGCG 1 cut(s) 816
AciI CCGC 8 cut(s) 27, 36, 39, 66, 102, 270, 273, 276
AclWI GGATC 2 cut(s) 5, 381
AcoI YGGCCR 4 cut(s) 3, 48, 54, 306
AcsI RAATTY 3 cut(s) 153, 346, 627
AcuI CTGAAG 4 cut(s) 15, 459, 642, 918
AfaI GTAC 3 cut(s) 221, 326, 872
AfiI CCNNNNNNNGG 3 cut(s) 87, 431, 641
AflIII ACRYGT 1 cut(s) 805
AluBI AGCT 7 cut(s) 62, 197, 320, 399, 536, 575, 932
AluI AGCT 7 cut(s) 62, 197, 320, 399, 536, 575, 932
Alw21I GWGCWC 2 cut(s) 591, 835
Alw26I GTCTC 1 cut(s) 663
Alw44I GTGCAC 1 cut(s) 587
AlwI GGATC 2 cut(s) 5, 381
AlwNI CAGNNNCTG 3 cut(s) 197, 302, 482
AoxI GGCC 9 cut(s) 3, 48, 54, 89, 238, 257, 306, 335, 433
ApaLI GTGCAC 1 cut(s) 587
ApeKI GCWGC 8 cut(s) 62, 137, 194, 197, 278, 358, 361, 452
ApoI RAATTY 3 cut(s) 153, 346, 627
Asp700I GAANNNNTTC 1 cut(s) 627
AspLEI GCGC 2 cut(s) 818, 889
AspS9I GGNCC 5 cut(s) 90, 159, 258, 336, 433
AsuC2I CCSGG 1 cut(s) 256
AsuHPI GGTGA 6 cut(s) 171, 299, 574, 656, 666, 731
AvaII GGWCC 1 cut(s) 159
AxyI CCTNAGG 2 cut(s) 235, 459
BaeGI GKGCMC 1 cut(s) 591
BalI TGGCCA 2 cut(s) 5, 308
BarI GAAGNNNNNNTAC 4 cut(s) 645, 677, 837, 869
Bbv12I GWGCWC 2 cut(s) 591, 835
BbvCI CCTCAGC 1 cut(s) 759
BbvI GCAGC 8 cut(s) 49, 149, 184, 206, 265, 370, 373, 439
BccI CCATC 4 cut(s) 178, 337, 478, 563
BceAI ACGGC 4 cut(s) 26, 63, 69, 89
BcgI CGANNNNNNTGC 2 cut(s) 303, 337
BcnI CCSGG 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 663
BfaI CTAG 2 cut(s) 879, 929
BfmI CTRYAG 1 cut(s) 394
BglI GCCNNNNNGGC 1 cut(s) 47
BlpI GCTNAGC 1 cut(s) 400
Bme1390I CCNGG 1 cut(s) 256
Bme18I GGWCC 1 cut(s) 159
BmgT120I GGNCC 5 cut(s) 90, 159, 258, 336, 433
BmiI GGNNCC 3 cut(s) 161, 338, 434
BmrFI CCNGG 1 cut(s) 256
BmrI ACTGGG 1 cut(s) 290
BmsI GCATC 2 cut(s) 197, 637
BmuI ACTGGG 1 cut(s) 290
Bpu10I CCTNAGC 1 cut(s) 759
Bpu1102I GCTNAGC 1 cut(s) 400
BpuMI CCSGG 1 cut(s) 256
BsaAI YACGTR 1 cut(s) 586
BsaI GGTCTC 1 cut(s) 663
BsaJI CCNNGG 4 cut(s) 51, 241, 254, 903
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 431, 641
Bse1I ACTGG 2 cut(s) 231, 296
Bse21I CCTNAGG 2 cut(s) 235, 459
Bse3DI GCAATG 1 cut(s) 496
BseDI CCNNGG 4 cut(s) 51, 241, 254, 903
BseLI CCNNNNNNNGG 3 cut(s) 87, 431, 641
BseMI GCAATG 1 cut(s) 496
BseMII CTCAG 3 cut(s) 249, 450, 750
BseNI ACTGG 2 cut(s) 231, 296
BseRI GAGGAG 3 cut(s) 105, 402, 411
BseSI GKGCMC 1 cut(s) 591
BseX3I CGGCCG 1 cut(s) 54
BseXI GCAGC 8 cut(s) 49, 149, 184, 206, 265, 370, 373, 439
BseYI CCCAGC 1 cut(s) 14
Bsh1236I CGCG 1 cut(s) 816
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 9 cut(s) 5, 50, 56, 91, 240, 259, 308, 337, 435
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 2 cut(s) 591, 835
BsiSI CCGG 3 cut(s) 57, 256, 282
BslI CCNNNNNNNGG 3 cut(s) 87, 431, 641
BsmAI GTCTC 1 cut(s) 663
BsnI GGCC 9 cut(s) 5, 50, 56, 91, 240, 259, 308, 337, 435
Bso31I GGTCTC 1 cut(s) 663
Bsp1286I GDGCHC 2 cut(s) 591, 835
Bsp143I GATC 4 cut(s) 10, 386, 790, 925
Bsp1720I GCTNAGC 1 cut(s) 400
BspACI CCGC 8 cut(s) 27, 36, 39, 66, 102, 270, 273, 276
BspANI GGCC 9 cut(s) 5, 50, 56, 91, 240, 259, 308, 337, 435
BspCNI CTCAG 3 cut(s) 248, 451, 751
BspFNI CGCG 1 cut(s) 816
BspLI GGNNCC 3 cut(s) 161, 338, 434
BspPI GGATC 2 cut(s) 5, 381
BspTNI GGTCTC 1 cut(s) 663
BsrBI CCGCTC 1 cut(s) 68
BsrDI GCAATG 1 cut(s) 496
BsrI ACTGG 2 cut(s) 231, 296
BssECI CCNNGG 4 cut(s) 51, 241, 254, 903
BssMI GATC 4 cut(s) 10, 386, 790, 925
BssT1I CCWWGG 1 cut(s) 903
Bst4CI ACNGT 4 cut(s) 304, 593, 614, 915
Bst6I CTCTTC 4 cut(s) 123, 420, 666, 705
BstBAI YACGTR 1 cut(s) 586
BstC8I GCNNGC 1 cut(s) 885
BstDEI CTNAG 5 cut(s) 235, 400, 459, 532, 759
BstDSI CCRYGG 1 cut(s) 51
BstFNI CGCG 1 cut(s) 816
BstHHI GCGC 2 cut(s) 818, 889
BstKTI GATC 4 cut(s) 13, 389, 793, 928
BstMAI GTCTC 1 cut(s) 663
BstMBI GATC 4 cut(s) 10, 386, 790, 925
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 5 cut(s) 35, 47, 62, 194, 278
BstNSI RCATGY 1 cut(s) 809
BstSCI CCNGG 1 cut(s) 254
BstSFI CTRYAG 1 cut(s) 394
BstSLI GKGCMC 1 cut(s) 591
BstUI CGCG 1 cut(s) 816
BstV1I GCAGC 8 cut(s) 49, 149, 184, 206, 265, 370, 373, 439
BstX2I RGATCY 2 cut(s) 10, 386
BstYI RGATCY 2 cut(s) 10, 386
BstZI CGGCCG 1 cut(s) 54
Bsu36I CCTNAGG 2 cut(s) 235, 459
BsuRI GGCC 9 cut(s) 5, 50, 56, 91, 240, 259, 308, 337, 435
BtgI CCRYGG 1 cut(s) 51
BtsIMutI CAGTG 3 cut(s) 224, 300, 589
Cac8I GCNNGC 1 cut(s) 885
CaiI CAGNNNCTG 3 cut(s) 197, 302, 482
CfoI GCGC 2 cut(s) 818, 889
Cfr13I GGNCC 5 cut(s) 90, 159, 258, 336, 433
Csp6I GTAC 3 cut(s) 220, 325, 871
CviAII CATG 4 cut(s) 505, 600, 788, 806
CviQI GTAC 3 cut(s) 220, 325, 871
DdeI CTNAG 5 cut(s) 235, 400, 459, 532, 759
DpnI GATC 4 cut(s) 12, 388, 792, 927
DpnII GATC 4 cut(s) 10, 386, 790, 925
DraI TTTAAA 1 cut(s) 499
EaeI YGGCCR 4 cut(s) 3, 48, 54, 306
EagI CGGCCG 1 cut(s) 54
Eam1104I CTCTTC 4 cut(s) 123, 420, 666, 705
EarI CTCTTC 4 cut(s) 123, 420, 666, 705
EclXI CGGCCG 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 903
Eco31I GGTCTC 1 cut(s) 663
Eco47I GGWCC 1 cut(s) 159
Eco52I CGGCCG 1 cut(s) 54
Eco57I CTGAAG 4 cut(s) 15, 459, 642, 918
Eco81I CCTNAGG 2 cut(s) 235, 459
EcoO109I RGGNCCY 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 903
ErhI CCWWGG 1 cut(s) 903
FaeI CATG 4 cut(s) 508, 603, 791, 809
FaiI YATR 8 cut(s) 8, 506, 583, 601, 642, 686, 789, 807
FatI CATG 4 cut(s) 504, 599, 787, 805
FauI CCCGC 2 cut(s) 109, 277
FspBI CTAG 2 cut(s) 879, 929
GlaI GCGC 2 cut(s) 817, 888
GsaI CCCAGC 1 cut(s) 18
HaeIII GGCC 9 cut(s) 5, 50, 56, 91, 240, 259, 308, 337, 435
HapII CCGG 3 cut(s) 57, 256, 282
HhaI GCGC 2 cut(s) 818, 889
Hin1II CATG 4 cut(s) 508, 603, 791, 809
Hin6I GCGC 2 cut(s) 816, 887
HinP1I GCGC 2 cut(s) 816, 887
HpaII CCGG 3 cut(s) 57, 256, 282
HphI GGTGA 6 cut(s) 171, 299, 574, 656, 666, 731
Hpy166II GTNNAC 2 cut(s) 290, 589
Hpy188I TCNGA 3 cut(s) 159, 345, 920
Hpy188III TCNNGA 4 cut(s) 458, 482, 620, 936
Hpy8I GTNNAC 2 cut(s) 290, 589
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 7 cut(s) 350, 363, 417, 446, 681, 703, 905
HpyCH4III ACNGT 4 cut(s) 304, 593, 614, 915
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 3 cut(s) 376, 589, 866
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 47, 62, 194, 278
HpyF3I CTNAG 5 cut(s) 235, 400, 459, 532, 759
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 4 cut(s) 508, 603, 791, 809
HspAI GCGC 2 cut(s) 816, 887
Kzo9I GATC 4 cut(s) 10, 386, 790, 925
LmnI GCTCC 6 cut(s) 59, 73, 317, 460, 472, 572
Lsp1109I GCAGC 8 cut(s) 49, 149, 184, 206, 265, 370, 373, 439
LweI GCATC 2 cut(s) 197, 637
MaeI CTAG 2 cut(s) 879, 929
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 3 cut(s) 43, 298, 562
MalI GATC 4 cut(s) 12, 388, 792, 927
MbiI CCGCTC 1 cut(s) 68
MboI GATC 4 cut(s) 10, 386, 790, 925
MflI RGATCY 2 cut(s) 10, 386
MhlI GDGCHC 2 cut(s) 591, 835
MlsI TGGCCA 2 cut(s) 5, 308
MluCI AATT 4 cut(s) 153, 346, 627, 861
MluNI TGGCCA 2 cut(s) 5, 308
MmeI TCCRAC 1 cut(s) 414
Mox20I TGGCCA 2 cut(s) 5, 308
MroXI GAANNNNTTC 1 cut(s) 627
MscI TGGCCA 2 cut(s) 5, 308
MseI TTAA 3 cut(s) 498, 797, 839
Msp20I TGGCCA 2 cut(s) 5, 308
MspA1I CMGCKG 2 cut(s) 197, 278
MspI CCGG 3 cut(s) 57, 256, 282
MspR9I CCNGG 1 cut(s) 256
MvnI CGCG 1 cut(s) 816
MwoI GCNNNNNNNGC 5 cut(s) 35, 47, 62, 194, 278
NciI CCSGG 1 cut(s) 256
NdeII GATC 4 cut(s) 10, 386, 790, 925
NlaIII CATG 4 cut(s) 508, 603, 791, 809
NlaIV GGNNCC 3 cut(s) 161, 338, 434
NmeAIII GCCGAG 2 cut(s) 266, 313
NmuCI GTSAC 3 cut(s) 43, 298, 562
NspI RCATGY 1 cut(s) 809
PciI ACATGT 1 cut(s) 805
PcsI WCGNNNNNNNCGW 1 cut(s) 856
PdmI GAANNNNTTC 1 cut(s) 627
PflMI CCANNNNNTGG 1 cut(s) 641
Ppu21I YACGTR 1 cut(s) 586
PscI ACATGT 1 cut(s) 805
PspFI CCCAGC 1 cut(s) 14
PspN4I GGNNCC 3 cut(s) 161, 338, 434
PspPI GGNCC 5 cut(s) 90, 159, 258, 336, 433
PstNI CAGNNNCTG 3 cut(s) 197, 302, 482
PsuI RGATCY 2 cut(s) 10, 386
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 3 cut(s) 221, 326, 872
RsaNI GTAC 3 cut(s) 220, 325, 871
SaqAI TTAA 3 cut(s) 498, 797, 839
Sau3AI GATC 4 cut(s) 10, 386, 790, 925
Sau96I GGNCC 5 cut(s) 90, 159, 258, 336, 433
ScrFI CCNGG 1 cut(s) 256
SduI GDGCHC 2 cut(s) 591, 835
SfaNI GCATC 2 cut(s) 197, 637
SfcI CTRYAG 1 cut(s) 394
SinI GGWCC 1 cut(s) 159
Sse9I AATT 4 cut(s) 153, 346, 627, 861
SsiI CCGC 8 cut(s) 27, 36, 39, 66, 102, 270, 273, 276
SspMI CTAG 2 cut(s) 879, 929
StyD4I CCNGG 1 cut(s) 254
StyI CCWWGG 1 cut(s) 903
TaaI ACNGT 4 cut(s) 304, 593, 614, 915
TaiI ACGT 1 cut(s) 588
TaqI TCGA 2 cut(s) 550, 829
TaqII GACCGA 1 cut(s) 585
TasI AATT 4 cut(s) 153, 346, 627, 861
TauI GCSGC 5 cut(s) 38, 41, 68, 275, 278
Tru1I TTAA 3 cut(s) 498, 797, 839
Tru9I TTAA 3 cut(s) 498, 797, 839
TscAI CASTG 3 cut(s) 231, 307, 596
TseFI GTSAC 3 cut(s) 43, 298, 562
TseI GCWGC 8 cut(s) 62, 137, 194, 197, 278, 358, 361, 452
Tsp45I GTSAC 3 cut(s) 43, 298, 562
TspDTI ATGAA 1 cut(s) 238
TspRI CASTG 3 cut(s) 231, 307, 596
Van91I CCANNNNNTGG 1 cut(s) 641
VneI GTGCAC 1 cut(s) 587
VpaK11BI GGWCC 1 cut(s) 159
XapI RAATTY 3 cut(s) 153, 346, 627
XceI RCATGY 1 cut(s) 809
XmnI GAANNNNTTC 1 cut(s) 627
XspI CTAG 2 cut(s) 879, 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.